Publications by authors named "Chunhua Wang"

215 Publications

Development and Internal Validation of a Preoperative Prediction Model for Sentinel Lymph Node Status in Breast Cancer: Combining Radiomics Signature and Clinical Factors.

Front Oncol 2021 8;11:754843. Epub 2021 Nov 8.

Department of Radiology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Purpose: To develop and internally validate a nomogram combining radiomics signature of primary tumor and fibroglandular tissue (FGT) based on pharmacokinetic dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and clinical factors for preoperative prediction of sentinel lymph node (SLN) status in breast cancer patients.

Methods: This study retrospectively enrolled 186 breast cancer patients who underwent pretreatment pharmacokinetic DCE-MRI with positive ( = 93) and negative ( = 93) SLN. Logistic regression models and radiomics signatures of tumor and FGT were constructed after feature extraction and selection. The radiomics signatures were further combined with independent predictors of clinical factors for constructing a combined model. Prediction performance was assessed by receiver operating characteristic (ROC), calibration, and decision curve analysis. The areas under the ROC curve (AUCs) of models were corrected by 1,000-times bootstrapping method and compared by Delong's test. The added value of each independent model or their combinations was also assessed by net reclassification improvement (NRI) and integrated discrimination improvement (IDI) indices. This report referred to the "Transparent Reporting of a multivariable prediction model for Individual Prognosis Or Diagnosis" (TRIPOD) statement.

Results: The AUCs of the tumor radiomic model (eight features) and the FGT radiomic model (three features) were 0.783 (95% confidence interval [CI], 0.717-0.849) and 0.680 (95% CI, 0.604-0.757), respectively. A higher AUC of 0.799 (95% CI, 0.737-0.862) was obtained by combining tumor and FGT radiomics signatures. By further combining tumor and FGT radiomics signatures with progesterone receptor (PR) status, a nomogram was developed and showed better discriminative ability for SLN status [AUC 0.839 (95% CI, 0.783-0.895)]. The IDI and NRI indices also showed significant improvement when combining tumor, FGT, and PR compared with each independent model or a combination of any two of them (all < 0.05).

Conclusion: FGT and clinical factors improved the prediction performance of SLN status in breast cancer. A nomogram integrating the DCE-MRI radiomics signature of tumor and FGT and PR expression achieved good performance for the prediction of SLN status, which provides a potential biomarker for clinical treatment decision-making.
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http://dx.doi.org/10.3389/fonc.2021.754843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606782PMC
November 2021

G3BP2 regulated by the lncRNA LINC01554 facilitates esophageal squamous cell carcinoma metastasis through stabilizing HDGF transcript.

Oncogene 2021 Nov 15. Epub 2021 Nov 15.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, P. R. China.

Metastasis is the leading cause of death of patients with esophageal squamous cell carcinoma (ESCC). Although an increasing number of studies have demonstrated the involvement of G3BP2 in several human cancers, how G3BP2 interacts with long noncoding RNAs and regulates mRNA transcripts in mediating ESCC metastasis remains unclear. In this study, we uncovered that G3BP2 was upregulated in ESCC. Further analysis revealed that upregulation of G3BP2 was significantly correlated with lymph node metastasis, depth of tumor invasion and unfavorable outcomes in ESCC patients. Both in vitro and in vivo functional assays demonstrated that G3BP2 dramatically enhanced ESCC cell migration and invasion. Mechanistically, LINC01554 maintained the high G3BP2 expression in ESCC by protecting G3BP2 from degradation through ubiquitination and the interaction domains within LINC01554 and G3BP2 were identified. In addition, RNA-seq revealed that HDGF was regulated by G3BP2. G3BP2 bound to HDGF mRNA transcript to stabilize its expression. Ectopic expression of HDGF effectively abolished the G3BP2 depletion-mediated inhibitory effect on tumor cell migration. Intriguingly, introduction of compound C108 which can inhibit G3BP2 remarkedly suppressed ESCC cell metastasis in vitro and in vivo. Collectively, this study describes a newly discovered regulatory axis, LINC01554/G3BP2/HDGF, that facilitates ESCC metastasis and will provide novel therapeutic strategies for ESCC.
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http://dx.doi.org/10.1038/s41388-021-02073-0DOI Listing
November 2021

Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic FeO nanoparticles for magnetic targeting immunotherapy of solid tumors.

Nanoscale 2021 Nov 25;13(45):19109-19122. Epub 2021 Nov 25.

Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, P. R. China.

Although there has been significant progress in the development of tumor immunotherapies, many challenges still exist for the treatment of solid tumors. Natural killer (NK) cells possess broad-spectrum cytotoxicity against tumors, but their limited migration and infiltration abilities restrict their application in solid tumor therapies. Here, we combined a facile and efficient magnetic-targeting strategy with NK cell-based therapy to develop a novel immunotherapy approach for treating solid tumors. Anti-CD56 antibodies were conjugated with FeO nanoparticles, which could specifically bind with NK-92 cells endowing them with a magnetic field driven targeting ability. These NK-FeO biohybrid nanoparticles were able to facilitate directional migration to the tumor site under external magnetic field guidance and efficiently inhibit tumor growth. These functionalized NK cells represent a novel approach for solid tumor therapy and may provide a promising modality for cancer interventions in the future.
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http://dx.doi.org/10.1039/d1nr03329hDOI Listing
November 2021

A novel polymer electrolyte with high elasticity and high performance for lithium metal batteries.

Chem Commun (Camb) 2021 Nov 2;57(87):11493-11496. Epub 2021 Nov 2.

National Key Lab. of Power Sources, Tianjin Institute of Power Sources, Tianjin 300381, P. R. China.

A polymer electrolyte with high elasticity and high performance is prepared by polymerization. The polymer electrolyte is amorphous and has a high ionic conductivity of 7.9 × 10 S cm and good elasticity. The discharge capacity of Li/LiFePO in the 100th cycle is 133.90 mA h g (0.5C, 25 °C).
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http://dx.doi.org/10.1039/d1cc04110jDOI Listing
November 2021

Hydrogenative Ring-Rearrangement of Furfural to Cyclopentanone over Pd/UiO-66-NO with Tunable Missing-Linker Defects.

Molecules 2021 Sep 22;26(19). Epub 2021 Sep 22.

Department of Chemistry, Ghent University, Krijgslaan 281-S3, 9000 Ghent, Belgium.

Upgrading furfural (FAL) to cyclopentanone (CPO) is of great importance for the synthesis of high-value chemicals and biomass utilization. The hydrogenative ring-rearrangement of FAL is catalyzed by metal-acid bifunctional catalysts. The Lewis acidity is a key factor in promoting the rearrangement of furan rings and achieving a high selectivity to CPO. In this work, highly dispersed Pd nanoparticles were successfully encapsulated into the cavities of a Zr based MOF, UiO-66-NO, by impregnation using a double-solvent method (DSM) followed by H reduction. The obtained Pd/UiO-66-NO catalyst showed a significantly better catalytic performance in the aforementioned reaction than the Pd/UiO-66 catalyst due to the higher Lewis acidity of the support. Moreover, by using a thermal treatment. The Lewis acidity can be further increased through the creating of missing-linker defects. The resulting defective Pd/UiO-66-NO exhibited the highest CPO selectivity and FAL conversion of 96.6% and 98.9%, respectively. In addition, the catalyst was able to maintain a high activity and stability after four consecutive runs. The current study not only provides an efficient catalytic reaction system for the hydrogenative ring-rearrangement of furfural to cyclopentanone but also emphasizes the importance of defect sites.
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http://dx.doi.org/10.3390/molecules26195736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510385PMC
September 2021

Nicotinamide mononucleotide attenuates doxorubicin-induced cardiotoxicity by reducing oxidative stress, inflammation and apoptosis in rats.

Arch Biochem Biophys 2021 11 2;712:109050. Epub 2021 Oct 2.

Department of Radiology, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Doxorubicin (DOX) is an effective and widely used antineoplastic drug. However, its clinical application is limited due to its dose-dependent cardiotoxicity. Great efforts have been made to explore the pathological mechanism of DOX-induced cardiotoxicity (DIC), but new drugs and strategies to alleviate cardiac damage are still needed. Here, we aimed to investigate the effect of nicotinamide mononucleotide (NMN) on DIC in rats. The results of the present study showed that DOX treatment significantly induced cardiac dysfunction and cardiac injury, whereas NMN alleviated these changes. In addition, NMN inhibited Dox-induced activation of nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome-mediated inflammation, as evidenced by decreased caspase 1 and IL-1β activity. Moreover, NMN treatment increased glutathione (GSH) levels and superoxide dismutase (SOD) activity and decreased the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) in DOX-treated rats. Furthermore, NMN treatment mitigated DOX-induced cardiomyocyte apoptosis and cardiac fibrosis. In conclusion, the results indicated that NMN protects against DIC in rats by inhibiting NLRP3 inflammasome activation, oxidative stress, and apoptosis.
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http://dx.doi.org/10.1016/j.abb.2021.109050DOI Listing
November 2021

Global identification and determination of the major constituents in Kai-Xin-San by ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry and gas chromatography-mass spectrometry.

J Pharm Biomed Anal 2021 Nov 24;206:114385. Epub 2021 Sep 24.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 301617, China; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin, 301617, China. Electronic address:

Kai-Xin-San (KXS) is a traditional Chinese medicine (TCM) formula containing four herbal medicines: Ginseng Radix Rhizoma, Polygalae Radix, Poria and Acori Tatarinowii Rhizoma. A large number of pharmacological studies in vitro and in vivo have shown that KXS is characterized by anti-depression, anti-Alzheimer's disease, anti-oxidation and other activities. However, the pharmacodynamic substance basis studies of KXS are hitherto quite limited. Here, KXS was identified and determined by ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS) and gas chromatography-mass spectrometry (GC-MS). Firstly, the data-dependent acquisition mode (DDA) of UPLC-Q-Orbitrap MS combined with the inclusion list were used to collected the chemical composition. The chemical constituents of KXS were identified by local database on compound discoverer™ 3.1 software and Xcalibur 4.1 software. With the use of this approach, a total of 211 compounds were identified from KXS. Wherein 60 compounds were from Ginseng Radix Rhizoma, 40 compounds were from Poria, and 111 compounds were from Polygala Radix, respectively. Secondly, 105 volatile constituents were identified by GC-MS analysis, which were mainly derived from Acori Tatarinowii Rhizoma. Besides, an adjusted parallel reaction monitoring method was established and validated to quantify the seventeen major compounds in different herbal medicines of KXS, which were chosen as the benchmarked substances to evaluate the quality of KXS. In conclusion, this study provided a generally applicable strategy for global metabolite identification of the complicated components and determination of multi-component content in traditional Chinese medicines.
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http://dx.doi.org/10.1016/j.jpba.2021.114385DOI Listing
November 2021

Nicorandil Ameliorates Doxorubicin-Induced Cardiotoxicity in Rats, as Evaluated by 7 T Cardiovascular Magnetic Resonance Imaging.

Cardiovasc Drugs Ther 2021 Sep 30. Epub 2021 Sep 30.

Department of Radiology, West China Hospital, Sichuan University, No. 37 Guoxue Road, Chengdu, 610041, China.

Purpose: Doxorubicin-induced cardiotoxicity (DIC) is a common side effect of doxorubicin chemotherapy, and a major mechanism of DIC is inflammation. However, no effective method exists to prevent DIC. In the present study, we investigated the cardioprotective effects of nicorandil against DIC using multiparametric cardiac magnetic resonance (CMR) imaging and elucidated the anti-inflammatory properties of nicorandil in rat models.

Methods: Male Sprague-Dawley rats received four weekly intraperitoneal doxorubicin doses (4 mg/kg/injection) to establish the DIC model. After treatment with or without nicorandil (3 mg/kg/day) or diazoxide (10 mg/kg/day) orally, all the groups underwent weekly CMR examinations, including cardiac function and strain assessment and T2 mapping, for 6 weeks. Additionally, blood samples and hearts were collected to examine inflammation and histopathology.

Results: According to our results, the earliest DIC CMR parameter in the doxorubicin group was T2 mapping time prolongation compared with the DIC rats treated with nicorandil (doxorubicin+nicorandil group) at week 2. Subsequently, the left ventricular ejection fraction (LVEF) and global peak systolic myocardial strain in the doxorubicin group were significantly reduced, and nicorandil effectively inhibited these effects at week 6. Our results were confirmed by histopathological evaluations. Furthermore, nicorandil treatment had a protective effect against the doxorubicin-induced inflammatory response. Interestingly, similar protective results were obtained using the K channel opener diazoxide.

Conclusion: Collectively, our findings indicate that nicorandil application ameliorates DIC in rats with significantly higher cardiac function and myocardial strain and less fibrosis, apoptosis and inflammatory cytokine production. Nicorandil prevents T2 abnormalities in the early stages of DIC, showing a high clinical value for early nicorandil treatment in chemotherapy patients.
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http://dx.doi.org/10.1007/s10557-021-07252-5DOI Listing
September 2021

Mechanical Stress Induces a Transient Suppression of Cytokine Secretion in Astrocytes Assessed at the Single-Cell Level with a High-Throughput Microfluidic Chip.

Adv Healthc Mater 2021 11 22;10(21):e2100698. Epub 2021 Sep 22.

Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.

Brain cells are constantly subjected to mechanical signals. Astrocytes are the most abundant glial cells of the central nervous system (CNS), which display immunoreactivity and have been suggested as an emerging disease focus in the recent years. However, how mechanical signals regulate astrocyte immunoreactivity, and the cytokine release in particular, remains to be fully characterized. Here, human neural stem cells are used to induce astrocytes, from which the release of 15 types of cytokines are screened, and nine of them are detected using a protein microfluidic chip. When a gentle compressive force is applied, altered cell morphology and reinforced cytoskeleton are observed. The force induces a transient suppression of cytokine secretions including IL-6, MCP-1, and IL-8 in the early astrocytes. Further, using a multiplexed single-cell culture and protein detection microfluidic chip, the mechanical effects at a single-cell level are analyzed, which validates a concerted downregulation by force on IL-6 and MCP-1 secretions in the cells releasing both factors. This work demonstrates an original attempt of employing the protein detection microfluidic chips in the assessment of mechanical regulation on the brain cells at a single-cell resolution, offering novel approach and unique insights for the understanding of the CNS immune regulation.
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http://dx.doi.org/10.1002/adhm.202100698DOI Listing
November 2021

Ultrasensitive, high-throughput and multiple cancer biomarkers simultaneous detection in serum based on graphene oxide quantum dots integrated microfluidic biosensing platform.

Anal Chim Acta 2021 Sep 23;1178:338791. Epub 2021 Jun 23.

Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China. Electronic address:

Biomarkers play an important role in disease diagnosis and prognosis, which demand reliable, sensitive, rapid, and economic detection platform to conduct simultaneous multiple-biomarkers analysis in serum or body liquid. Here, we developed a universal biosensing platform through integrating the advantages of unique nanostructure and biochemistry properties of graphene oxide quantum dots and high throughput and low cost of microfluidic chip for reliable and simultaneous detection of multiple cancer antigen and antibody biomarkers. The performance of the proposed biosensing platform is validated through the representative cancer biomarkers including carcino-embryonic antigen (CEA), carbohydrate antigen 125 (CA125), α-fetoprotein (AFP), carbohydrate antigen 199 (CA199) and carbohydrate antigen 153 (CA153). It has a large linear quantification detection regime of 5-6 orders of magnitude and an ultralow detection limit of 1 pg/mL or 0.01 U/mL. Moreover, the proposed biosensing chip is capable of conducting 5-20 kinds of biomarkers from at least 60 persons simultaneously in 40 min with only 2 μL serum of each patient, which essentially reduces the detection cost and time to at least 1/60 of current popular methods. Clinical breast cancer and healthy samples detection results indicated its promising perspective in practical applications including cancer early diagnosis, prognosis, and disease pathogenesis study.
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http://dx.doi.org/10.1016/j.aca.2021.338791DOI Listing
September 2021

The curvature of cucumber fruits is associated with spatial variation in auxin accumulation and expression of a YUCCA biosynthesis gene.

Hortic Res 2020 Sep 1;7(1):135. Epub 2020 Sep 1.

College of Horticulture and Landscape Architecture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Harbin, 150030, China.

Fruit curving lowers the commercial value of cucumber and leads to significant economic losses. The mechanism driving the abnormal curving of cucumber is largely unknown. Through our previous work, we discovered that 2 days post-anthesis (DPA) was the key time point at which various phenotypic and genotypic characteristics of cucumber fruits are determined. Here, we analyzed the transcriptome of the concave (C1) and convex (C2) sides of curved fruits at 2 DPA by Gene Ontology (GO) enrichment and functional pathway enrichment analyses and identified auxin as a putative factor influencing fruit curvature. Changes in the curve angle in the fruits and exogenous auxin treatment analyses showed that asymmetric auxin distribution induces fruit curving. Identification of differentially expressed genes (DEGs) related to auxin and qPCR validation showed that CsYUC10b had the most significant differential expression when both sides of the curved fruits were compared. Gene functional analysis showed that the transcript levels of CsYUC10b and the auxin concentration were even on both sides of the fruit in CsYUC10b-overexpressing plants, which in turn contributed to an equal rate of growth of both sides of cucumber fruits and resulted in a straight shape of the fruits. Thus, we conclude that CsYUC10b promotes the formation of straight cucumber fruits, with possible applications in the production and breeding of cucumber.
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http://dx.doi.org/10.1038/s41438-020-00354-5DOI Listing
September 2020

Phytochemical Constituents and Biological Activities of Plants from the Genus Cissampelos.

Chem Biodivers 2021 Oct 31;18(10):e2100358. Epub 2021 Aug 31.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Cissampelos is a significant genus comprising of approximately 21 species of the medicinal plants (Menispermaceae). The plants of this genus are used in traditional medicine for the treatment of various ailments such as asthma, arthritis, dysentery, hyperglycemia, cardiopathy, hypertension and other related problems. These plants are rich in bioactive dibenzylisoquinoline and aborphine as well as small amounts of other ingredients. In recent years, the chemical constituents and pharmacological activities of Cissampelos genus have been paid more and more attention due to their diversity. Herein, we compile the chemical constituents and biological activities on this genus, and summarize the C-NMR data of the main bioactive ingredients. All information comes from scientific databases such as Google Scholar, PubMed, Sci-Finder, ScienceDirect, Web of Science and CNKI. It provides valuable data for the future research and development of Cissampelos genus.
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http://dx.doi.org/10.1002/cbdv.202100358DOI Listing
October 2021

Investigating the preventive effects of 99Tc-methylenediphosphonate on glucocorticoid-induced osteoporosis rabbit model.

Curr Top Med Chem 2021 Aug 3. Epub 2021 Aug 3.

Department of Rheumatology and Immunology, Heze Municipal Hospital, Shandong Province. China.

Background And Objective: Osteoporosis is a worldwide healthcare challenge. Conventional medications for osteoporosis prevention are not clinically effective or associated with gastrointestinal tract adverse effects. The present study aimed to comparatively investigate the effects of technetium-99 conjugated with methylene diphosphonate (99Tc-MDP) and calcium carbonate and alendronate in prevention and treatment of osteoporosis in glucocorticoid-induced osteoporosis rabbit model through evaluating bone alkaline phosphatase (B-ALP), TRAP-5b levels and histopathological parameters.

Method: Forty healthy female New Zealand rabbits were randomly divided into five groups (each n=8), including control group (Control Group), osteoporosis model group (GIO Group), osteoporosis model + 99Tc-MDP group (99Tc-MDP Group), osteoporosis model + alendronate group (Alendronate Group), and osteoporosis model + calcium carbonate group (calcium carbonate Group). Animals in each group were treated with corresponding interventions for 14 weeks. The blood samples were collected at the first and 14th week, and B-ALP and TRAP-5b levels were detected by enzyme-linked immunosorbent assay (ELISA). The rabbits were anesthetized at the 14th week, and pathological cytological observation was performed on both femurs.

Results: Age and weights of rabbits in different groups had no statistically significant differences (P>0.05). B-ALP levels in serum of all groups except for Control Group decreased after treatment, but the differences were not statistically significant (P>0.05). TRAP-5b levels in serum of all groups increased after treatment. Specifically, differences in the GIO Group and Calcium carbonate Group were statistically significant (P<0.05), while differences in 99Tc-MDP Group and Alendronate‎ Group were not statistically significant (P<0.05). Pathological sections revealed that Control Group presented normal bone tissue morphology. The bone tissue morphology of the 99Tc-MDP Group and Alendronate Group was similar to Control Group and GIO Group. Moreover, Calcium carbonate Group and GIO Group exhibited similar bone tissue morphology.

Conclusions: 99Tc-MDP has preventive effect on the glucocorticoid-induced osteoporotic rabbit model. This osteoporosis preventive effect might be attributed to the capacities of 99Tc-MDP in promoting the osteoblasts generation and inhibiting the generation and reducing the activity of osteoclasts.

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http://dx.doi.org/10.2174/1568026621666210804114744DOI Listing
August 2021

An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications.

Polymers (Basel) 2021 Jul 14;13(14). Epub 2021 Jul 14.

Department of Biomass and Leather Engineering, Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China.

Decades of research into cryogels have resulted in the development of many types of cryogels for various applications. Collagen and gelatin possess nontoxicity, intrinsic gel-forming ability and physicochemical properties, and excellent biocompatibility and biodegradability, making them very desirable candidates for the fabrication of cryogels. Collagen-based cryogels (CBCs) and gelatin-based cryogels (GBCs) have been successfully applied as three-dimensional substrates for cell culture and have shown promise for biomedical use. A key point in the development of CBCs and GBCs is the quantitative and precise characterization of their properties and their correlation with preparation process and parameters, enabling these cryogels to be tuned to match engineering requirements. Great efforts have been devoted to fabricating these types of cryogels and exploring their potential biomedical application. However, to the best of our knowledge, no comprehensive overviews focused on CBCs and GBCs have been reported currently. In this review, we attempt to provide insight into the recent advances on such kinds of cryogels, including their fabrication methods and structural properties, as well as potential biomedical applications.
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http://dx.doi.org/10.3390/polym13142299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309424PMC
July 2021

Direct epitaxial growth of nickel phosphide nanosheets on nickel foam as self-support electrode for efficient non-enzymatic glucose sensing.

Nanotechnology 2021 Aug 6;32(43). Epub 2021 Aug 6.

Faculty of Materials Science and Chemistry, China University of Geosciences, 68 Jincheng Street, East Lake High-tech Development Zone, Wuhan 430074, People's Republic of China.

Design and develop of cost-effective non-enzymatic electrode materials is of great importance for next generation of glucose sensors. In this work, we report a high-performance self-supporting electrode fabricated via direct epitaxial growth of nickel phosphide on Ni foam (NiP/NF) for nonenzymatic glucose sensors in alkaline solution. Under the optimal conditions, the uniform NiP nanosheets could be obtained with an average thickness of 80 nm, which provides sufficient active sites for glucose molecules. As a consequence, the NiP/NF electrode displays superior electrochemistry performances with a high sensitivity of 6375.1A mMcm, a quick response about 1 s, a low detection limit of 0.14M (S/N = 3), and good selectivity and specificity. Benefit from the strong interaction between NiP and NF, the NiP/NF electrode is also highly stable for long-term applications. Furthermore, the NiP/NF electrode is capable of analyzing glucose in human blood serum with satisfactory results, indicating that the NiP/NF is a potential candidate for glucose sensing in real life.
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http://dx.doi.org/10.1088/1361-6528/ac162fDOI Listing
August 2021

Ultrasensitive, high-throughput, and rapid simultaneous detection of SARS-CoV-2 antigens and IgG/IgM antibodies within 10 min through an immunoassay biochip.

Mikrochim Acta 2021 07 20;188(8):262. Epub 2021 Jul 20.

Institute of Marine Science and Technology, Shandong University, Qingdao, 266000, China.

COVID-19 is now a severe threat to global health. Facing this pandemic, we developed a space-encoding microfluidic biochip for high-throughput, rapid, sensitive, simultaneous quantitative detection of SARS-CoV-2 antigen proteins and IgG/IgM antibodies in serum. The proposed immunoassay biochip integrates the advantages of graphene oxide quantum dots (GOQDs) and microfluidic chip and is capable of conducting multiple SARS-CoV-2 antigens or IgG/IgM antibodies of 60 serum samples simultaneously with only 2 μL sample volume of each patient. Fluorescence intensity of antigens and IgG antibody detection at emission wavelength of ~680 nm was used to quantify the target concentration at excitation wavelength of 632 nm, and emission wavelength of ~519 nm was used during the detection of IgM antibodies at excitation wavelength of 488 nm. The method developed has a large linear quantification detection regime of 5 orders of magnitude, an ultralow detection limit of ~0.3 pg/mL under optimized conditions, and less than 10-min qualitative detection time. The proposed biosensing platform will not only greatly facilitate the rapid diagnosis of COVID-19 patients, but also provide a valuable screening approach for infected patients, medical therapy, and vaccine recipients.
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http://dx.doi.org/10.1007/s00604-021-04896-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289455PMC
July 2021

Taurochenodeoxycholic acid inhibits the proliferation and invasion of gastric cancer and induces its apoptosis.

J Food Biochem 2021 Jul 18:e13866. Epub 2021 Jul 18.

Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

Taurochenodeoxycholic acid (TCDCA) is the principal ingredient of Compound Shougong Powder. Despite traditional Chinese medicine (TCM) research demonstrates that Compound Shougong Powder can restrict tumor growth, whether TCDCA exerts a role in suppressing cancer as the major ingredient of Compound Shougong Powder remains unknown. This study aims to clarify the regulatory mechanism of TCDCA on gastric cancer. Gastric cancer cells SGC-7901 were cultured to investigate the effects of TCDCA on proliferation and apoptosis. Furthermore, a subcutaneously implanted tumor model was established using SGC-7901 cells in BALB/C nude mice and tumor volume was measured under low and high dose treatment of TCDCA. Cell proliferation, apoptosis, and invasion were subjected to 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, flow cytometry, and transwell assay. Differentially expressed genes were screened by transcriptome sequencing. Nude mouse tumorigenicity assay was initiated to identify the effect of TCDCA on both tumor volume and weight, and the expression of candidate genes screened by transcriptome sequencing was determined by real-time fluorescence quantification (qPCR) and Western blot. The experiments revealed that TCDCA could significantly inhibit the proliferation and invasion of gastric cancer cells and induce apoptosis of these cells. Meanwhile, test findings via in vivo indicated that TCDCA severely diminished the volume and weight of tumors. This study first demonstrated that TCDCA inhibited the proliferation and invasion of gastric cancer and induced apoptosis, which is expected to serve as an experimental basis for the application of TCM in tumor therapeutic options. PRACTICAL APPLICATIONS: Through this study, the inhibitory effect of Taurochenodeoxycholic acid on gastric cancer can be clarified, which provides a new research basis for the application of traditional Chinese medicine (TCM) and TCM monomer in cancer. In addition, this study can further promote the research and application of Chinese traditional medicine, which has important application value and economic benefits.
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http://dx.doi.org/10.1111/jfbc.13866DOI Listing
July 2021

Separation of three chromones from Saposhnikovia divaricata using macroporous resins followed by preparative high-performance liquid chromatography.

J Sep Sci 2021 Sep 18;44(17):3287-3294. Epub 2021 Jul 18.

School of Pharmacy, the Key Laboratory of Prescription Effect and Clinical valuation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai, P. R. China.

Prim-O-glucosylcimifugin, cimifugin, and 5-O-methylvisamminoside are three major chromone derivatives of Saposhnikovia divaricata that have many pharmacological activities, such as anti-inflammatory and antitumor activities. In the present work, an effective method for the simultaneous separation of prim-O-glucosylcimifugin, cimifugin, and 5-O-methylvisamminoside with high purities was established using HPD-300 resin coupled with preparative high-performance liquid chromatography. The adsorption kinetics curves of the three compounds on the HPD-300 resin were studied and found to fit well according to the pseudo-second-order equation. The adsorption isotherm results indicated that the adsorption process of the three compounds was exothermic. After a one-run treatment with the resin, the contents of prim-O-glucosylcimifugin, cimifugin, and 5-O-methylvisamminoside increased from 0.29, 0.06, and 0.37% to 13.07, 2.83, and 16.91% with recovery yields of 76.38, 78.25, and 76.73%, respectively. Finally, the purities of the three compounds were found to reach more than 95% after further separation using preparative high-performance liquid chromatography. The method developed in this study was effective and could simultaneously separate three chromones from Saposhnikovia divaricate. The experimental results also showed that the HPD-300 resin is suitable for the separation of chromone derivatives.
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http://dx.doi.org/10.1002/jssc.202100345DOI Listing
September 2021

Celastrol inhibits rheumatoid arthritis through the ROS-NF-κB-NLRP3 inflammasome axis.

Int Immunopharmacol 2021 Sep 17;98:107879. Epub 2021 Jun 17.

Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, School of Pharmacy, Binzhou Medical University, Yantai 264003, Shandong, China. Electronic address:

Emerging evidence indicates that NOD-like receptor protein 3 (NLRP3) inflammasome-induced inflammation plays a critical role in the pathogenesis of rheumatoid arthritis (RA). Celastrol (Cel) is a quinone-methylated triterpenoid extracted from Tripterygium wilfordii that is used to treat RA. However, researchers have not determined whether Cel exerts anti-RA effects by regulating the activation of the NLRP3 inflammasome. In the present study, complete Freund's adjuvant (CFA)- induced rats and human mononuclear macrophages (THP-1 cells) were used to explore the anti-RA effects of Cel and its underlying mechanism. Joint swelling, the arthritis index score, inflammatory cell infiltration, and synovial hyperplasia in CFA-induced rats were correspondingly reduced after Cel treatment. The secretion of interleukin (IL)-1β and IL-18 in the serum of CFA-induced rats and supernatants of THP-1 cells exposed to Cel was significantly decreased. These inhibitory effects occurred because Cel blocked the nuclear factor-kappa B (NF-κB) signaling pathway and inhibited the activation of the NLRP3 inflammasome. Furthermore, Cel inhibited reactive oxygen species (ROS) production induced by lipopolysaccharide (LPS) and adenosine triphosphate (ATP). We speculated that Cel relieves RA symptoms and inhibits inflammation by inhibiting the ROS-NF-κB-NLRP3 axis.
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http://dx.doi.org/10.1016/j.intimp.2021.107879DOI Listing
September 2021

Base-promoted, CBr-mediated tandem bromination/intramolecular Friedel-Crafts alkylation of N-aryl enamines: a facile access to 1H- and 3H-indoles.

Org Biomol Chem 2021 Jun 28;19(24):5377-5382. Epub 2021 May 28.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P. R. China. and State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P. R. China.

Described here is a general and highly efficient method for the synthesis of 1H- and 3H-indoles. In the presence of CBr and a suitable base, the cyclization of N-aryl enamines proceeds with high efficiency. Unlike previous intramolecular cross dehydrogenative coupling (CDC) of the same substrates, this process does not require the use of either a transition metal or a stoichiometric amount of oxidant. This method also features operational simplicity, easy scalability and good substrate tolerability. Control experiments indicate the reactions may proceed in a tandem sequence of bromination and intramolecular Friedel-Crafts alkylation in a simple one-pot procedure.
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http://dx.doi.org/10.1039/d1ob00731aDOI Listing
June 2021

Refinery wastewater treatment via a multistage enhanced biochemical process.

Sci Rep 2021 05 13;11(1):10282. Epub 2021 May 13.

School of Chemistry and Materials Engineering, Huizhou University, 3# Building, Huizhou, 516007, People's Republic of China.

Petroleum refinery wastewater (PRWW) that contains recalcitrant components as the major portion of constituents is difficult to treat by conventional biological processes. An effective and economical biological treatment process was established to treat industrial PRWW with an influent COD of over 2500 mg L in this research. This process is mainly composed of internal circulation biological aerated filter (ICBAF), hydrolysis acidfication (HA), two anaerobic-aerobic (A/O) units, a membrane biological reactor (MBR), and ozone-activated carbon (O-AC) units. The results showed that, overall, this system removed over 94% of the COD, BOD ammonia nitrogen (NH-N) and phosphorus in the influent, with the ICBAF unit accounting for 54.6% of COD removal and 83.6% of BOD removal, and the two A/O units accounting for 33.3% of COD removal and 9.4% of BOD removal. The degradation processes of eight organic pollutants and their removal via treatment were also analyzed. Furthermore, 26 bacteria were identified in this system, with Proteobacteria and Acidobacteria being the most dominant. Ultimately, the treatment process exhibited good performance in degrading complex organic pollutants in the PRWW.
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http://dx.doi.org/10.1038/s41598-021-89665-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119716PMC
May 2021

Diagnostic performance of T2-weighted imaging and intravoxel incoherent motion diffusion-weighted MRI for predicting metastatic axillary lymph nodes in T1 and T2 stage breast cancer.

Acta Radiol 2021 Mar 27:2841851211002834. Epub 2021 Mar 27.

Division of Radiology, 92293Sichuan Cancer Hospital and Research Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology, Chengdu 61004, Sichuan, PR China.

Background: Non-invasive modalities for assessing axillary lymph node (ALN) are needed in clinical practice.

Purpose: To investigate the suspicious ALN on unenhanced T2-weighted (T2W) imaging and intravoxel incoherent motion diffusion-weighted imaging (IVIM DWI) for predicting ALN metastases (ALNM) in patients with T1-T2 stage breast cancer and clinically negative ALN.

Material And Methods: Two radiologists identified the most suspicious ALN or the largest ALN in negative axilla by T2W imaging features, including short axis (Size-S), long axis (Size-L)/S ratio, fatty hilum, margin, and signal intensity on T2W imaging. The IVIM parameters of these selected ALNs were also obtained. The Mann-Whitney U test or t-test was used to compare the metastatic and non-metastatic ALN groups. Finally, logistic regression analysis with T2W imaging and IVIM features for predicting ALNM was conducted.

Results: This study included 49 patients with metastatic ALNs and 50 patients with non-metastatic ALNs. Using the above conventional features on T2W imaging, the sensitivity and specificity in predicting ALNM were not high. Compared with non-metastatic ALNs, metastatic ALNs had lower pseudo-diffusion coefficient (D*) ( = 0.043). Logistic regression analysis showed that the most useful features for predicting ALNM were signal intensity and D*. The sensitivity and specificity predicting ALNM that satisfied abnormal signal intensity and lower D* were 73.5% and 84%, respectively.

Conclusions: The abnormal signal intensity on T2W imaging and one IVIM feature (D*) were significantly associated with ALNM, with sensitivity of 73.5% and specificity of 84%.
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http://dx.doi.org/10.1177/02841851211002834DOI Listing
March 2021

Enrichment-Detection Integrated Exosome Profiling Biosensors Promising for Early Diagnosis of Cancer.

Anal Chem 2021 03 12;93(11):4697-4706. Epub 2021 Mar 12.

Institute of Marine Science and Technology, Shandong University, Qingdao 266237, China.

Enrichment-detection integrated biosensors for exosome profiling have shown great potential in noninvasive diagnosis and point-of-care testing with the advantage of multifunctions. This Feature focuses on the enrichment-detection integrated exosome profiling biosensors emphasizing (i) the underlying working fundamentals of these sorts of biosensors, (ii) four advanced strategies developed for exosome analysis, and (iii) future outlook and present challenges of exosome profiling systems.
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http://dx.doi.org/10.1021/acs.analchem.0c05245DOI Listing
March 2021

Favorable Electrochemical Performance of LiMnO/LiFePO Composite Electrodes Attributed to Composite Solid Electrolytes for All-Solid-State Lithium Batteries.

Langmuir 2021 Feb 8;37(7):2349-2354. Epub 2021 Feb 8.

Science and Technology on Power Sources Laboratory, Tianjin Institute of Power Sources, Tianjin 300384, P.R. China.

LiAlGe(PO) (LAGP)-PEO composite electrolytes are unstable in LiMnO. In addition, the discharge platform potential (2.8 and 4.0 V) difference of LiMnO is relatively large, whereas the discharge platform potential (3.5 V) of LiFePO is between 2.8 and 4.0 V. Thus, LiMnO and LiFePO can be compounded together to reduce the material platform voltage difference and obtain the advantages of both materials at the same time. Here, LiMnO/LiFePO composite cathodes were applied in solid-state batteries. LAGP-PEO(LiTFSI) was used as the electrolyte. The Li/composite cathode battery using composite electrolytes has a reversible capacity of 192.8 mAh g at 50 °C and 0.1 C. It possesses favorable rate performance and exhibits very good cycling stability. In addition, the composite electrolytes can prevent the further occurrence of the Jahn-Teller effect. Meanwhile, the charge-transfer resistance slightly decreases in 10 cycles. The excellent capacity retention of the battery is connected with the excellent electrochemical stability and the well-interfaced contacts of the composite electrolytes with electrodes.
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http://dx.doi.org/10.1021/acs.langmuir.0c03274DOI Listing
February 2021

Efficacy of Treating Chronic Tibial Osteomyelitis With Bone Defect Using a Pedicled Perforator-Layered Flap and Fasciocutaneous Flap of the Posterior Tibial Artery: A Case Report.

Wounds 2020 Nov;32(11):E50-E54

The Fourth Affiliated Hospital of Anhui Medical University, Xinzhan District, Hefei, Anhui, China.

Introduction: Tibial osteomyelitis is a common complication of bone tissue trauma. Obtaining good soft tissue coverage and effective infection management is key to the treatment of chronic osteomyelitis of the tibia accompanied with bone defect and bone exposure. The pedicled posterior tibial artery perforator layered fasciocutaneous flap can be used to repair soft tissue defects and can be used as a long-term, localized anti-infective.

Case Report: A 54-year-old male presented with an ulcer, purulent discharge at the left anterior tibia, and a fever 28 years after complete healing of the scar site. The patient received debridement and negative pressure wound therapy (NPWT) in a hospital setting. After presenting to the authors' department, there was difficulty in closing the exposed bone marrow cavity. On the basis of systemic use of intravenous antibiotics, multiple debridements and NPWT were used to effectively remove necrotic tissue and control infection. Afterward, the pedicled posterior tibial artery perforator layered fasciocutaneous flap was designed to fill the bone marrow cavity as well as cover and seal the wound of bone exposure and soft tissue defect simultaneously. The layered fasciocutaneous flap was well established after operation, and no recurrence of osteomyelitis was found.

Conclusion: Debridement with negative pressure wound therapy can be an effective treatment for the wound bed preparation in advance of surgery, and the pedicled posterior tibial artery perforator layered fasciocutaneous flap can be used for the treatment of several soft tissue defects.
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November 2020

Additive-Free Copper(I)-Mediated Synthesis of 5- or 6-Brominated 2-Aryl-1-Indole-3-Carboxylates from α,α-Dibromo β-Iminoesters.

J Org Chem 2021 01 4;86(2):1964-1971. Epub 2021 Jan 4.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P. R. China.

Additive-free copper(I)-bromide-mediated radical cyclization reactions of α,α-dibromo β-iminoesters were investigated, enabling the synthesis of a series of 5- or 6-brominated 2-aryl-1-indole-3-carboxylates in moderate to good yields. The mechanistic study showed that (i) the bromine atom originated from the substrates and (ii) the bromination might be related to a 3-bromo-3 indole intermediate via an electrophilic bromine atom transfer. Furthermore, the practicality of this method was demonstrated by gram-scale synthesis and the potential for product derivatization toward other valuable multisubstituted indoles.
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http://dx.doi.org/10.1021/acs.joc.0c02497DOI Listing
January 2021

Memristive Circuit Implementation of a Self-Repairing Network Based on Biological Astrocytes in Robot Application.

IEEE Trans Neural Netw Learn Syst 2021 Feb 4;PP. Epub 2021 Feb 4.

A large number of studies have shown that astrocytes can be combined with the presynaptic terminals and postsynaptic spines of neurons to constitute a triple synapse via an endocannabinoid retrograde messenger to achieve a self-repair ability in the human brain. Inspired by the biological self-repair mechanism of astrocytes, this work proposes a self-repairing neuron network circuit that utilizes a memristor to simulate changes in neurotransmitters when a set threshold is reached. The proposed circuit simulates an astrocyte-neuron network and comprises the following: 1) a single-astrocyte-neuron circuit module; 2) an astrocyte-neuron network circuit; 3) a module to detect malfunctions; and 4) a neuron PR (release probability of synaptic transmission) enhancement module. When faults occur in a synapse, the neuron module becomes silent or near silent because of the low PR of the synapses. The circuit can detect faults automatically. The damaged neuron can be repaired by enhancing the PR of other healthy neurons, analogous to the biological repair mechanism of astrocytes. This mechanism helps to repair the damaged circuit. A simulation of the circuit revealed the following: 1) as the number of neurons in the circuit increases, the self-repair ability strengthens and 2) as the number of damaged neurons in the astrocyte-neuron network increases, the self-repair ability weakens, and there is a significant degradation in the performance of the circuit. The self-repairing circuit was used for a robot, and it effectively improved the robots' performance and reliability.
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http://dx.doi.org/10.1109/TNNLS.2020.3041624DOI Listing
February 2021

An SHR-SCR module specifies legume cortical cell fate to enable nodulation.

Nature 2021 01 9;589(7843):586-590. Epub 2020 Dec 9.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai, China.

Legumes, unlike other plants, have the ability to establish symbiosis with nitrogen-fixing rhizobia. It has been theorized that a unique property of legume root cortical cells enabled the initial establishment of rhizobial symbiosis. Here we show that a SHORTROOT-SCARECROW (SHR-SCR) stem cell program in cortical cells of the legume Medicago truncatula specifies their distinct fate. Regulatory elements drive the cortical expression of SCR, and stele-expressed SHR protein accumulates in cortical cells of M. truncatula but not Arabidopsis thaliana. The cortical SHR-SCR network is conserved across legume species, responds to rhizobial signals, and initiates legume-specific cortical cell division for de novo nodule organogenesis and accommodation of rhizobia. Ectopic activation of SHR and SCR in legumes is sufficient to induce root cortical cell division. Our work suggests that acquisition of the cortical SHR-SCR module enabled cell division coupled to rhizobial infection in legumes. We propose that this event was central to the evolution of rhizobial endosymbiosis.
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http://dx.doi.org/10.1038/s41586-020-3016-zDOI Listing
January 2021

Androgen receptor suppresses vasculogenic mimicry in hepatocellular carcinoma via circRNA7/miRNA7-5p/VE-cadherin/Notch4 signalling.

J Cell Mol Med 2020 12 28;24(23):14110-14120. Epub 2020 Oct 28.

School of Life Sciences, Anhui Medical University, Hefei, China.

Androgen receptor (AR) can suppress hepatocellular carcinoma (HCC) invasion and metastasis at an advanced stage. Vasculogenic mimicry (VM), a new vascularization pattern by which tumour tissues nourish themselves, is correlated with tumour progression and metastasis. Here, we investigated the effect of AR on the formation of VM and its mechanism in HCC. The results suggested that AR could down-regulate circular RNA (circRNA) 7, up-regulate micro RNA (miRNA) 7-5p, and suppress the formation of VM in HCC Small hairpin circR7 (ShcircR7) could reverse the impact on VM and expression of VE-cadherin and Notch4 increased by small interfering AR (shAR) in HCC, while inhibition of miR-7-5p blocked the formation of VM and expression of VE-cadherin and Notch4 decreased by AR overexpression (oeAR) in HCC. Mechanism dissection demonstrated that AR could directly target the circR7 host gene promoter to suppress circR7, and miR-7-5p might directly target the VE-cadherin and Notch4 3'UTR to suppress their expression in HCC. In addition, knockdown of Notch4 and/or VE-cadherin revealed that shVE-cadherin or shNotch4 alone could partially reverse the formation of HCC VM, while shVE-cadherin and shNotch4 together could completely suppress the formation of HCC VM. Those results indicate that AR could suppress the formation of HCC VM by down-regulating circRNA7/miRNA7-5p/VE-Cadherin/Notch4 signals in HCC, which will help in the design of novel therapies against HCC.
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http://dx.doi.org/10.1111/jcmm.16022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754040PMC
December 2020
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