Publications by authors named "Chun-Yan Yang"

56 Publications

Family Dynamics and Grandparents' Anxiety and Depression in Intergenerational Rearing Families: A Correlational Study.

Front Psychiatry 2021 20;12:633773. Epub 2021 May 20.

Geriatric Psychiatry Department, Shanghai Mental Health Center, Shanghai, China.

In China, intergenerational rearing is a ubiquitous phenomenon based on unique national conditions. This study aimed to explore family dynamics in intergenerational rearing families as well as their correlation with older household members' anxiety and depression. The elderly from intergenerational ( = 141) and non-intergenerational rearing families ( = 266) were investigated using the following scales: the general information questionnaire, Self-Rating Scale of Systemic Family Dynamics, Geriatric Depression Scale, and Self-Rating Anxiety Scale. Scores from the four dimensions (family atmosphere, system logic, individuation, and the concept of disease) of the structure of family dynamics were computed. The comparison of these dimensions scores and the total scores of grandparents' anxiety and depression for the two groups were not statistically significant ( > 0.05). In Pearson's correlation analysis, no significant correlation between the family atmosphere dimension and the total score of the grandparents' depression and anxiety scales was observed. The system logic aspect was negatively correlated with depression and anxiety scale scores. The individual dimension was positively correlated with the anxiety scale scores. The disease concept dimension was positively correlated with depression and anxiety scale scores. Hence, the results were statistically significant. There were no significant differences in terms of family dynamics and risk of anxiety and depression among grandparents between the two family types. The system logic, individuation, and disease concept dimensions were correlated with their anxiety and depression.
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http://dx.doi.org/10.3389/fpsyt.2021.633773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172783PMC
May 2021

Molecular signatures of in vitro produced embryos derived from ovum pick up or slaughterhouse oocytes in buffalo.

Theriogenology 2021 Jul 13;169:14-20. Epub 2021 Apr 13.

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China. Electronic address:

This study was performed to investigate the difference in developmental competence of oocytes derived from ovum pick-up (OPU) and slaughterhouse ovaries (SLH), and its underlying mechanisms. The OPU and SLH oocytes were in-vitro maturated and fertilized to produce blastocysts, and these blastoycsts were collected to explore the expression of key genes for developmental potential and telomere (Oct-4, Sox2, Nanog, Cdx2, Gata3, E-cadherin, β-catenin, TERT, TERF1 and TERF2). The results showed that both the cleavage and blastocyst rates were significantly higher for the OPU group (68.31%, 39.48%, respectively) than SLH group (57.59%, 26.50%, respectively) (P < 0.01). The relative mRNA abundances of Sox2, Oct-4, Nanog and E-cadherin were significantly higher in the OPU blastocysts than the SLH ones (P < 0.01). Protein expression analysis by Western blot and immunofluorescence also revealed that the expression of E-cadherin and Sox2 was significantly higher in OPU blastocysts than SLH ones. However, there was no significant differences between the two groups in the expression of Cdx2, β-catenin, Gata3, TERT, TERF1, TERF2. These results imply oocyte sources modify the expression of development and adhesion related genes in blastocysts, which may elucidate a possible reasoning for the low development competence of buffalo SLH embryos.
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http://dx.doi.org/10.1016/j.theriogenology.2021.03.025DOI Listing
July 2021

Xanthoceraside induces cell apoptosis through downregulation of the PI3K/Akt/Bcl-2/Bax signaling pathway in cell lines of human bladder cancer.

Indian J Pathol Microbiol 2021 Apr-Jun;64(2):294-301

Department of Urology, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui, China.

Background: Xanthoceraside is a component obtained in the husks of Xanthoceras sorbifolia Bunge. Series of researches proved that xanthoceraside had functions of anti-inflammation and anti-tumor effects. However, the mechanisms of xanthoceraside against bladder cancer are unclear. Accordingly, we proposed to investigate xanthoceraside's impacts and potential mechanisms in cells of bladder cancer.

Methods: By using the CCK-8 assay, we measured the viability of cells. With the use of 4,6-diamidino-2-phenylindole (DAPI) staining, we examined nuclear fragmentation and chromatin condensation in the nuclei of apoptotic cells. By using flow cytometry, we measured cell apoptosis. By using Western blotting, we tested the expressions of Caspase-9, Caspase-8, Caspase-3, Bcl-xL, P53, and PI3K/Akt/Bcl-2/Bax.

Results: The proliferation of cell lines of human bladder cancer T24 and 5637 was suppressed by xanthoceraside significantly in a time- and concentration-dependent way. When cell lines 5637 and T24 were incubated as the xanthoceraside dose increased, the rates of cell apoptosis were upregulated, which was dependent on dose. According to further analysis, xanthoceraside induced apoptosis by upregulating Bax and downregulating the expression of Bcl-xL and Bcl-2. However, xanthoceraside did not change the expression of Caspase-9, Caspase-8, and Caspase-3. Interestingly, xanthoceraside also downregulated the expression of p-PI3K and p-Akt, and upregulated P53.

Conclusions: Xanthoceraside induces cell apoptosis through downregulation of the PI3K/Akt/Bcl-2/Bax signaling pathway in cell lines of human bladder cancer.
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http://dx.doi.org/10.4103/IJPM.IJPM_462_19DOI Listing
April 2021

[Risk factors for neutropenia of late newborns].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Apr;23(4):375-380

Department of Neonatology, Xuzhou Central Hospital/Xuzhou School of Clinical Medicine of Nanjing Medical University, Xuzhou, Jiangsu 221009, China.

Objective: To study the risk factors and treatment for neutropenia of late newborns (NLN).

Methods: Related clinical data were collected from the preterm infants and critically ill neonates who were admitted to the neonatal intensive care unit from July 2019 to January 2020. A total of 46 newborns with a blood absolute neutrophil count (ANC) of < 1.5×10/L for two consecutive times at weeks 2-4 after birth were enrolled as the NLN group. A total of 92 late newborns with a blood ANC of ≥ 1.5×10/L, matched at a ratio of 1:2, were enrolled as the control group. Possible risk factors associated with NLN and the treatment process were recorded. A logistic regression analysis was performed to identify the risk factors for NLN.

Results: Among the 46 neonates in the NLN group, 29 had a gestational age of < 32 weeks, 14 had a gestational age of 32-37 weeks, and 3 had a gestational age of > 37 weeks. There was no significant difference between the two groups in the incidence rates of gestational hypertension, premature rupture of membranes > 18 hours and intrauterine distress, 5-minute Apgar score, the duration of positive pressure ventilation, the incidence rate of early-onset sepsis, and the type of initially used antibiotics ( > 0.05). Compared with the control group, the NLN group had a higher incidence rate of late-onset sepsis and a longer duration of antibiotic use ( < 0.05). Late-onset sepsis and prolonged duration of antibiotic use were independent risk factors for NLN ( < 0.05). With the presence of late-onset sepsis, the risk of NLN was increased by 1.537 times in neonates, and the risk of NLN was increased by 76.9% for every 3-day increase in the duration of antibiotic use. The mean age at the diagnosis of NLN was (21±6) days for the 46 neonates in the NLN group. Thirteen neonates with NLN were administered with recombinant human granulocyte colony-stimulating factor (G-CSF, 10 μg/kg) once or twice. O the 13 neonates, 6 had an ANC of < 0.5×10/L and 7 had a gestational age of < 32 weeks or severe disease conditions. After treatment the ANC returned to > 1.0×10/L in the 13 neonates. No drug-related adverse reactions were found. After the diagnosis of NLN, 2 neonates developed sepsis, and the remaining 44 neonates did not develop any common purulent infections.

Conclusions: The risk of NLN increases with the presence of late-onset sepsis and the increase in the duration of antibiotic use. NLN is generally a benign process. G-CSF appears to be safe and effective for NLN with severe disease conditions or severe reduction in ANC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050555PMC
April 2021

[Horizontal penetration needling method for headache].

Zhongguo Zhen Jiu 2020 Nov;40(11):1193-7

Department of Encephalopathy, Nanjing Hospital of TCM, Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province.

The manipulation and key points of professor -'s horizontal penetration needling method for headache were introduced. This acupuncture method selects local acupoints of head, including occipital acupoint group, temporal acupoint group, frontal acupoint group and vertex acupoint group. The needles are shallowly and horizontally inserted at acupoints, with needle end towards the focus or headache site. The needle retaining time is long (6 h). This method is commonly used in the treatment of migraine, cervicogenic headache and tension-type headache. The manipulation is standard with better repeatability.
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http://dx.doi.org/10.13703/j.0255-2930.20200114-k0001DOI Listing
November 2020

Effect of zinc chloride and sodium selenite supplementation on maturation, oxidative biomarkers, and gene expression in buffalo () oocytes.

Zygote 2021 Mar 26:1-8. Epub 2021 Mar 26.

Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt.

This study examined the effects of zinc chloride (ZnCl2) and sodium selenite (Na2SeO3) supplementation in maturation medium on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. Ovaries from a slaughterhouse were aspirated and good quality cumulus-oocyte complexes (COCs) with at least four layers of compact cumulus cells and evenly granulated dark ooplasm were selected. COCs were randomly allocated during IVM (22 h) to one of four treatment groups: (1) control maturation medium (basic medium), or basic medium supplemented with (2) ZnCl2 (1.5 µg/ml), (3) Na2SeO3 (5 µg/l), or (4) ZnCl2 + Na2SeO3 (1.5 µg/ml + 5 µg/l, respectively). Oocytes were denuded after 22 h of IVM in the first four replicates. Specimens were fixed and stained to evaluate the stage of nuclear maturation. The spent medium was collected for biochemical assays of total antioxidant capacity (TAC), malondialdehyde (MDA) and hydrogen peroxide concentrations. A second four replicates were used for COCs for RNA extraction. The expression levels of antioxidant (SOD1, GPX4, CAT and PRDX1), antiapoptotic (BCL2 and BCL-XL) and proapoptotic (BAX and BID) genes were measured. Supplementation with ZnCl2 and Na2SeO3 during IVM increased the ratio of oocytes reaching metaphase II at 22 h, increased TAC and decreased MDA and H2O2 concentrations in the maturation medium (P < 0.05). Moreover, beneficial effects were associated with complementary changes in expression patterns of antioxidative, antiapoptotic and proapoptotic genes, suggesting lower oxidative stress and apoptosis. Supplementation medium with zinc chloride and sodium selenite improves the maturation rate, reduces oxidative stress and increases expression levels of antioxidative and antiapoptotic genes.
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http://dx.doi.org/10.1017/S0967199421000162DOI Listing
March 2021

Molecular, functional, and cellular alterations of oocytes and cumulus cells induced by heat stress and shock in animals.

Environ Sci Pollut Res Int 2020 Nov 6;27(31):38472-38490. Epub 2020 Aug 6.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.

Global warming is considered as the main environmental stress affecting ecosystems as well as physiological and biochemical characteristics, and survivability of living organisms. High temperature induces various stresses and causes reduction of fertility through reducing the oocyte developmental competence and alteration in surrounding cells' functions. This causes major economic loss to livestock creating a selective pressure on animals to the advantage of better adapted genotypes and to the detriment of others. In this review, a search in Science Direct, Google Scholar, PubMed, Web of Science, Scopus, and SID databases until 2020 was conducted. Keywords which include heat stress, shock, high temperature, oocyte, cumulus, and animals were investigated. Studies have exhibited that heat stress can disturb the development and function of oocyte and cumulus cells (CCs) concerning reproductive efficiency. Heat stress has deleterious consequences on oocyte maturation and development via reduced number of polar body extrusion, adenosine monophosphate, and guanosine monophosphate synthesis. Heat stress caused the alteration of cytoplasmic and nuclear features as well as trans-zonal projections and gap junctions. In addition, heat stress is accompanied with reduced mitochondrial activity (copy mDNA number, distribution, and membrane potential) in cumulus-oocyte complexes. This review targets the description of results in the most recent studies that aimed to call attention to the influences of heat stress on molecular, functional, and cellular changes in oocytes and CCs in animals to design evidence on the acting mechanisms as the core of this problem from a comparative review.
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http://dx.doi.org/10.1007/s11356-020-10302-4DOI Listing
November 2020

[Revelation of 's subcutaneous needling therapy on the development of acupuncture and moxibustion].

Zhongguo Zhen Jiu 2020 Mar;40(3):307-14

Department of Cerebropathy, Nanjing TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province.

By analyzing 's subcutaneous needling phenomenon, the attribute of 's subcutaneous needling therapy in traditional acupuncture, the innovation and the role in the development of traditional acupuncture were demonstrated. The authors believe that the de-meridian in 's subcutaneous needling therapy is similar to the connotation of meridian before the Han dynasty, the de-acupoint phenomenon in 's subcutaneous needling therapy reproduces the acupoint evolution, and the sites of needle insertion of this therapy are partially overlapped with the location of traditional acupoints. In manipulation, the technique of 's subcutaneous needling therapy is derived from the traditional acupuncture technique and it is also in reference to the theory and the method of modern medicine, such as muscle energy technology and fasciology. The needle device and the sweeping technique are the most essential innovation of 's subcutaneous needling therapy. 's subcutaneous needling therapy has the distinct attribute of traditional acupuncture.
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http://dx.doi.org/10.13703/j.0255-2930.20190223-k0005DOI Listing
March 2020

[Analysis on relationship between syndrome differentiation of migraine and acupuncture effect].

Zhongguo Zhen Jiu 2020 Jan;40(1):96-102

Department of Encephalopathy, Affiliated Nanjing TCM Hospital of Nanjing University of CM, Nanjing 210001, Jiangsu Province.

The problems of the syndrome differentiation of migraine in acupuncture treatment were collected, e.g. inconsistency of syndrome differentiation, unclear staging of syndrome differentiation, lack of standardization in comparison between syndrome differentiation and non-differentiation, insufficient research on the factors of syndrome differentiation. In view of the exiting problems, focusing on two aspects of migraine, namely syndrome differentiation and acupuncture effect, the clinical treatment and research are conducted in migraine treated with acupuncture based on syndrome differentiation. It is believed that the comprehensive observation of the relationship between syndrome differentiation and acupuncture effect, as well as the analysis of the relevant factors of syndrome differentiation of migraine should be the focus in future research.
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http://dx.doi.org/10.13703/j.0255-2930.20190126-k0002DOI Listing
January 2020

Effect of season on the in-vitro maturation and developmental competence of buffalo oocytes after somatic cell nuclear transfer.

Environ Sci Pollut Res Int 2020 Mar 6;27(7):7729-7735. Epub 2020 Jan 6.

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, China.

Somatic cell nuclear transfer (SCNT) is a valuable technology tool with various uses in transgenic animals, regenerative medicine, and stem cell research. However, the efficiency of SCNT embryos appears to have poor developmental competency. Environmental issues may adversely affect SCNT embryos in buffalo. Thereafter, the present study aimed to explore the effect of season on the maturation of buffalo oocytes and subsequent developmental capability after parthenogenetic activation and SCNT in buffalo. Buffalo oocytes (n = 6353) were collected from local slaughterhouse at various seasons; spring (March-April), summer (May-August), autumn (September-November), and winter (December-January). A significant increase (p < 0.05) was recorded in the maturation rate (57.07%) at autumn compared with spring, summer, and winter (50.46, 50.93, and 50.66%, respectively). No significant differences were recorded in the fusion and the cleavage rates among all seasons. Blastocyst development rate was higher (p < 0.05) in autumn and winter (16.52 ± 8.45% and 15.98 ± 7.17%, respectively) than in spring and summer (9.47 ± 6.71% and 10.84 ± 6.58%, respectively) seasons. It could be concluded that the season had a significant effect on oocyte development competence which can be used for SCNT in buffalo.
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http://dx.doi.org/10.1007/s11356-019-07470-3DOI Listing
March 2020

A Glycine max sodium/hydrogen exchanger enhances salt tolerance through maintaining higher Na efflux rate and K/Na ratio in Arabidopsis.

BMC Plant Biol 2019 Nov 5;19(1):469. Epub 2019 Nov 5.

State Key Laboratory of North China Crop Improvement and Regulation, Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, 071000, Hebei, China.

Background: Soybean (Glycine max (L.)) is one the most important oil-yielding cash crops. However, the soybean production has been seriously restricted by salinization. It is therefore crucial to identify salt tolerance-related genes and reveal molecular mechanisms underlying salt tolerance in soybean crops. A better understanding of how plants resist salt stress provides insights in improving existing soybean varieties as well as cultivating novel salt tolerant varieties. In this study, the biological function of GmNHX1, a NHX-like gene, and the molecular basis underlying GmNHX1-mediated salt stress resistance have been revealed.

Results: We found that the transcription level of GmNHX1 was up-regulated under salt stress condition in soybean, reaching its peak at 24 h after salt treatment. By employing the virus-induced gene silencing technique (VIGS), we also found that soybean plants became more susceptible to salt stress after silencing GmNHX1 than wild-type and more silenced plants wilted than wild-type under salt treatment. Furthermore, Arabidopsis thaliana expressing GmNHX1 grew taller and generated more rosette leaves under salt stress condition compared to wild-type. Exogenous expression of GmNHX1 resulted in an increase of Na transportation to leaves along with a reduction of Na absorption in roots, and the consequent maintenance of a high K/Na ratio under salt stress condition. GmNHX1-GFP-transformed onion bulb endothelium cells showed fluorescent pattern in which GFP fluorescence signals enriched in vacuolar membranes. Using the non-invasive micro-test technique (NMT), we found that the Na efflux rate of both wild-type and transformed plants after salt treatment were significantly higher than that of before salt treatment. Additionally, the Na efflux rate of transformed plants after salt treatment were significantly higher than that of wild-type. Meanwhile, the transcription levels of three osmotic stress-related genes, SKOR, SOS1 and AKT1 were all up-regulated in GmNHX1-expressing plants under salt stress condition.

Conclusion: Vacuolar membrane-localized GmNHX1 enhances plant salt tolerance through maintaining a high K/Na ratio along with inducing the expression of SKOR, SOS1 and AKT1. Our findings provide molecular insights on the roles of GmNHX1 and similar sodium/hydrogen exchangers in regulating salt tolerance.
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http://dx.doi.org/10.1186/s12870-019-2084-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833268PMC
November 2019

iTRAQ and PRM-based quantitative proteomics in early recurrent spontaneous abortion: biomarkers discovery.

Clin Proteomics 2019 18;16:36. Epub 2019 Oct 18.

3Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004 China.

Background: Early recurrent spontaneous abortion (ERSA) is a common condition in pregnant women. To prevent ERSA is necessary to look for abortion indicators, such as hormones and proteins, in an early stage.

Methods: Thirty patients with ERSA were enrolled in the case group. In the control group, we recruited 30 healthy women without a history of miscarriage undergoing voluntary pregnancy termination. The differentially expressed proteins in the serum were identified between the two groups using PRM and iTRAQ.

Results: Seventy-eight differentially expressed proteins were identified. Using GO functional annotation and KEGG pathway analysis, we detected that the most significant changes occurred in the pathway of Fc gamma R-mediated phagocytosis. Meanwhile, using PRM, we identified three proteins that were closely related to abortion, B4DTF1 (highly similar to PSG1), P11464 (PSG1), and B4DF70 (highly similar to Prdx-2). The levels of B4DTF1 and P11464 were down-regulated, while the level of B4DF70 was up-regulated.

Conclusions: CD45, PSG1, and Prdx-2, were significantly dysregulated in the samples of ERSA and could become important biomarkers for the prediction and diagnosis of ERSA. Larger‑scale studies are required to confirm the diagnostic value of these biomarkers.
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http://dx.doi.org/10.1186/s12014-019-9256-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798364PMC
October 2019

Disruption of epidermal permeability barrier enhances UV-induced hyperpigmentation.

Photodermatol Photoimmunol Photomed 2020 03 15;36(2):156-158. Epub 2019 Oct 15.

Department of Dermatology, First Affiliated Hospital of Kunming Medical University, Institute of Dermatology & Venereology of Yunnan Province, Kunming, China.

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http://dx.doi.org/10.1111/phpp.12515DOI Listing
March 2020

Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats.

Neurotox Res 2019 Oct 25;36(3):441-451. Epub 2019 May 25.

Department of Anatomy, College of Basic Medicine, Kunming Medical University, Kunming, 650500, Yunnan, People's Republic of China.

Mature brain-derived neurotrophic factor (mBDNF) has neuroprotection in cerebral ischemia. Conversely, the precursor of brain-derived neurotrophic factor (proBDNF) has the opposite function to its mature form, inducing apoptosis. However, whether the neuroprotection of Panax notoginsenoside Rb1 (PNS-Rb1) on ischemic stroke is due to, at least partially, its modulation of suppressing proBDNF/P75NTR/sortilin or upregulation of mBDNF is not clear. To test this hypothesis, rats induced by photothrombotic stroke were treated with PNS-Rb1 100 mg/kg or nimodipine 1 mg/kg twice a day until 3, 7, and 14 days. Our data indicate that PNS-Rb1 significantly reduced cerebral infarction rate, proBDNF/P75NTR/sortilin, and plasminogen activator inhibitor-1 (PAI-1) protein levels, and improved sensorimotor dysfunctions induced by ischemic stroke, upregulation of BDNF/TrkB levels, and its processing enzymes (tissue plasminogen activator, tPA) in a time-dependent manner. Taken together, our findings indicate that the improvement of sensorimotor dysfunctions by PNS-Rb1 following ischemic stroke is made, at least partially, by activating the BDNF/TrkB and inhibiting proBDNF/sortilin/P75NTR.
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http://dx.doi.org/10.1007/s12640-019-00058-2DOI Listing
October 2019

Early Premature Infant Oral Motor Intervention Improved Oral Feeding and Prognosis by Promoting Neurodevelopment.

Am J Perinatol 2020 05 23;37(6):626-632. Epub 2019 Apr 23.

Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong, China.

Objective: This study aimed to explore the clinical significance of early premature infant oral motor intervention (PIOMI) in the prognosis of premature infants.

Study Design: Infants were randomly divided into an intervention group ( = 78) and a control group ( = 73). PIOMI was given to the intervention group 15 to 30 minutes before feeding once a day for 14 days. The whole procedure lasted 15 minutes, including oral stimulation and nonnutritive sucking. Oral feeding ability and neuromotor development were evaluated using the Preterm Infant Oral Feeding Readiness Assessment (PIOFRA) scale and Infant Neurological International Battery (Infanib) scale.

Results: The PIOFRA score was higher in the intervention group and increased with time, showing a group-time interaction effect. The intervention group exhibited a higher feeding efficiency, a shorter transition time from assisted oral feeding to independent oral feeding, and lower body weight at achievement of independent oral feeding. The percentages of infants with a normal score on the Infanib scale were higher in the intervention group at 3 and 6 months of age, and an abnormal ratio was lower in the intervention group at 6 months (< 0.01).

Conclusion: PIOMI promoted neuromotor coordination by improving neurodevelopment, thereby improving the oral feeding ability and prognosis of preterm infants.
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http://dx.doi.org/10.1055/s-0039-1685448DOI Listing
May 2020

[Relationship between Quantitative Monitoring of WT1 Gene and Prognosis in Patients with Acute Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Dec;26(6):1632-1636

Department of Hematology, The Affiliated Hospital of Jining Medical College, Jining 272029, Shandong Province, China.

Objective: To investigate the exprassion of WT1 gene in patients with adult acute myeloid leukemia (AML) and its clinical significance.

Methods: Sixty-three newly diagnosed patients with acute myeloid leukemia were selected. Quantitative RT-PCR was used to detect the expression of WT1 gene in the 63 AML patients and 20 non-AML controls.

Results: WT1 gene was highly expressed in AML patents and its expression in the low-risk group was significantly lower than that in middle-risk group and high-risk group (P<0.05), and no significant difference of WT1 gene expression between middle-risk and high-risk group was observed. In the patients of middle-risk and high-risk patients, the expression of WT1 gene in the remission group was significantly lower than that in the patients of non-remission after treatment (P<0.05). The non-remission patients after first treatment in middle-risk and high-risk group were treated with second induction therapy. After second induction therapy, the WT1 expression in remission patients was significantly decreased (P<0.05) in comparison with that in patients still in non-remission. There was a negative correlation between WT1 expression and the 2-year overall survival rate in the newly diagnosed middle and high-risk AML patients.

Conclusion: The detection of WT1 gene expression can help to divide AML patients into low-/middle-/high-risk groups and to evaluate therapeutic response and clinical prognosis in middle and high-risk AML patients.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2018.06.009DOI Listing
December 2018

Vitrification alters cell adhesion related genes in pre-implantation buffalo embryos: Protective role of β-mercaptoethanol.

Theriogenology 2019 Feb 20;125:317-323. Epub 2018 Nov 20.

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, PR China. Electronic address:

The objectives of the present study were to investigate the effect of vitrification on the expression of the key genes associated with blastocyst developmental potential (β-catenin, E-cadherin, Oct-4, Cdx2, Gata3), and whether the presence of β-mercaptoethanol (β-ME, 100 μM) in in vitro culture (IVC) media will affect the expression of these genes. Buffalo pre-implantation embryos were divided into three groups: (1) fresh non-vitrified embryos were used as control, (2) vitrified embryos cultured with β-ME (+), and (3) vitrified embryos cultured without (-) β-ME. The results showed that all genes were affected by vitrification, however, the presence of β-ME in IVC media significantly (P < 0.05) modified the expression level of β-catenin, E-cadherin and Oct-4 in vitrified blastocyst compared to those cultured without β-ME. Protein expression analysis by immunofluorescence and western blot also revealed that the expression level of β-catenin and E-cadherin was significantly higher in vitrified embryos cultured with β-ME than those cultured without β-ME, which, in turn, was lower than fresh control group. However, there was no significant difference between vitrified groups in the expression level of Cdx2 and Gata3. Furthermore, the reduced rate of apoptosis in embryos cultured with β-ME confirms its role in protecting vitrified blastocyst against stress. In summary, vitrification alters the expression of the adhesion related genes in vitrified blastocyst, which may explain, at least in part, the reason for the low pregnancy rate following transfer of such embryos into recipient animal, and the supplementation of IVC media with β-ME significantly improved the quality of vitrified blastocyst evidenced by the modulation of the expression of blastocyst important genes, β-catenin, E-cadherin and Oct-4, and the ability to protect vitrified blastocyst against apoptosis.
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http://dx.doi.org/10.1016/j.theriogenology.2018.11.013DOI Listing
February 2019

[Distribution Characteristics of Sulfonamides and Sulfamethoxazole-Resistant Bacteria in Tannery Wastewater Treatment Processes].

Huan Jing Ke Xue 2018 Sep;39(9):4229-4235

College of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.

The study investigated abundance and distribution of three sulfonamides and sulfonamide-resistant bacteria in the wastewater treatment processes of two tannery wastewater plants, as well as the extent of their removal in different treatment units of the plants. Results showed that the three sulfonamides were detected in two different tannery wastewater treatment processes, with total content in water samples of 59.1-706.7 ng·L. The content of sulfonamides in excess sludge samples from the two plants was 4388 ng·kgand 2979.4 ng·kg, respectively. Antibiotic content was therefore similar to that in municipal wastewater treatment plants. The three sulfonamides were removed in two different sewage treatment processes; both led to removal extents>70%. Antibiotic removal extent was relatively high in the biological unit, especially in anaerobic tanks (removal extent>50%). Eight strains of resistant bacteria were screened out from influent, effluent, and excess sludge samples of the two plants. These were identified as five kinds of sulfonamide-resistant bacteria. The content of resistant bacteria in influent and effluent was between 9.37×10-5.08×10 CFU·mLand the content of sulfonamides was 1.17×10 CFU·gand 7.2×10 CFU·gin excess sludge samples. The removal extent of sulfamethoxazole-resistant bacteria in the two plants was 1.34 log and 2.15 log, respectively.
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http://dx.doi.org/10.13227/j.hjkx.201710173DOI Listing
September 2018

Developmental competence of buffalo (Bubalus bubalis) denuded oocytes cocultured with cumulus cells: Protective role of cumulus cells.

Theriogenology 2018 Oct 29;120:40-46. Epub 2018 Jul 29.

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, 530001, PR China. Electronic address:

The objectives of the present study were to evaluate the developmental competence of buffalo denuded oocytes (DOs) cocultured with cumulus cells (CCs) during in vitro maturation, and to investigate the mechanisms by which CCs promote oocyte maturation and development. Buffalo oocytes were matured in vitro for 24 h in three groups: (1) intact cumulus-oocyte complexes (COCs) (2) DOs cocultured with CCs (DOsCC), and (3) DOs cultured alone (DOs). Matured oocytes were used to determine the relative mRNA abundance of Gdf-9, Bmp15, Zar1, Caspase-3, Bcl-2, Zp2, Zp3, Cd9 and Pde3a by Rt-qPCR and CASPASE-3 protein expression by immunofluorescence. The intracellular content of cGMP, cAMP and MPF activity and the rate of embryonic development were also assessed. Results of the present study showed that in DOs, the relative mRNA abundance of Gdf-9, Bmp15, and Cd9 significantly (P < 0.05) decreased, whereas Caspase-3 (mRNA and protein levels), Bcl-2, and Pde3a exhibited higher expression than DOsCC and COCs. However, there was no significant difference among the groups in the expression level of Zar-1, Zp2, and Zp3. The intracellular content of cAMP and MPF activity was notably higher (P < 0.05) in DOs compared to COCs and DOsCC. There was no significant difference between COCs and DOsCC in cGMP content, which was significantly lower (P < 0.05) in DOs. Moreover, the cleavage and blastocyst rates were 58.4 ± 1.8%, 43.7 ± 1.1%, 18.4 ± 0.9% and 18.0 ± 1.3%, 11.0 ± 0.9% and 4.5 ± 0.6% in COCs, DOsCC and DOs groups, respectively. In conclusion, the presence of CCs protects buffalo DOs from apoptosis and promotes maturation through regulation of the intracellular content of cAMP and MPF activity and improves the fertilizing capacity of oocytes through modulation of the gamete fusion gene, Cd9.
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http://dx.doi.org/10.1016/j.theriogenology.2018.07.021DOI Listing
October 2018

Buffalo oocyte-secreted factors promote cumulus cells apoptosis and the rate of cGMP production but not steroidogenesis.

Reprod Domest Anim 2018 Dec 22;53(6):1523-1529. Epub 2018 Oct 22.

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, China.

The objectives of this study were to investigate the effect of buffalo oocyte-secreted factors (OSFs) on cumulus cells (CCs) functions, apoptosis and cGMP generation, and whether the direct contact between oocyte and CCs is essential for oocyte-mediated regulation of CCs functions. Buffalo CCs were cultured during IVM within three groups: (a) intact cumulus-oocyte complexes (COCs), (b) CCs cocultured with denuded oocytes (DOs) (CCs + DOs) and (c) CCs monolayer cultured alone (CCsM). After 24 hr of IVM, CCs were harvested for evaluation of the relative mRNA abundance of the genes encoding gap junction (GJA1), glycolysis (PFKP and LDHA), apoptosis (CASPASE-3 and BCL-2) and steroidogenesis (ER-β and PGR) by QRT-PCR, and CASPASE-3 proteins, using western blot. Intracellular cGMP content was also assessed by ELISA. Results showed that the relative abundance of LDHA, PFKP and BCL-2 significantly increased (p < 0.05) in COCs, whereas GJA1 and CASPASE-3 exhibited lower expression (p < 0.05) compared to CCs + DOs and CCsM groups. However, the expression levels of CASPASE-3, both mRNA and protein, were significantly (p < 0.05) downregulated in CCs + DOs compared to CCsM. There was no significant difference in the expression level of PGR and ER-β between the groups. The intracellular content of cGMP was notably (p < 0.05) higher in COCs compared to CCs + DOs and CCsM groups. In conclusion, this study demonstrated, for the first time, that buffalo OSFs protect CCs against apoptosis and stimulate their cGMP production; however, the regulation of cumulus glycolysis and gap junction is confined to those in close contact with the oocyte. Neither OSFs from COCs nor those from DOs have any effect on CCs steroidogenesis.
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http://dx.doi.org/10.1111/rda.13295DOI Listing
December 2018

[Clinical characteristics of hemophagocytic syndrome: analysis of 46 cases].

Nan Fang Yi Ke Da Xue Xue Bao 2018 Jun;38(6):769-inside back cover

Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 5105015, China. E-mail:

Objective: To analyze the clinical manifestations, laboratory findings, treatment and prognosis of patients with hemophagocytic syndrome (HPS).

Methods: A retrospective study was carried out to analyze the underlying disease, clinical characteristics, laboratory findings and outcomes of 46 patients with HPS.

Results: This cohort included 19 cases of HPS secondary to cancer, 11 cases of HPS secondary to infection, 10 cases of suspected malignant lymphoma based on PET-CT findings (without biopsy), and 6 cases of unknown etiology. The coincidence rate of the clinical characteristics of the patients with the indices listed in HPS-2004 criteria were: fever (100%), elevated serum ferritin (100%), cytopenias (93.48%), splenomegaly (91.30%), hemophagocytosis in the bone marrow, spleen or lymph nodes (84.78%), hypofibrinogenemia (67.39%), and hypertriglyceridemia (54.05%). The cases of cancer, infections and unknown etiology showed significant differences in serum levels of ferritin and β2MG (P<0.05), and significant differences were found in triglycerides, LDH, and fibrinogenemia between the nonfatal and fatal cases (P<0.05).

Conclusion: HPS can be secondary to various underlying diseases, many associated with Epstein-Barr virus infection. Cancer, especially NK/T-cell lymphoma, is the main cause of HPS. Persistent fever, elevated serum ferritin level and cytopenias are the most sensitive indicators for diagnosis of HPS, and early diagnosis and treatment are critical to lower the mortality rate of this disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765713PMC
June 2018

Astragaloside IV downregulates the expression of MDR1 in Bel‑7402/FU human hepatic cancer cells by inhibiting the JNK/c‑Jun/AP‑1 signaling pathway.

Mol Med Rep 2017 Sep 5;16(3):2761-2766. Epub 2017 Jul 5.

School of Pharmacy, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.

Previous studies demonstrated that astragaloside IV (ASIV) is a potential P‑glycoprotein (P‑gp)‑mediated multidrug resistance (MDR) reversal agent through mechanisms involving downregulation of the gene expression of mdr1. In order to investigate whether the c‑Jun N‑terminal kinase (JNK) signaling pathway is involved in the mechanism underlying ASIV‑induced downregulated the expression of mdr1, the present study used 5‑fluorouracil‑resistant Bel‑7402/FU human hepatic cancer cells as target cells. ASIV (0.1 mM) decreased the protein expression of phosphorylated (p)‑JNK and p‑c‑Jun in the Bel‑7402/FU cells, as determined using western blot analysis. Treatment with the JNK pathway inhibitor, SP600125, at a concentration of 11 µM, decreased the mRNA expression levels of mdr1 and P‑gp, as determined using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, and similar effects were observed following exposure to ASIV. Furthermore, electrophoretic mobility shift assays demonstrated that the DNA‑binding activity of activator protein‑1 (AP‑1) was decreased by 0.1 mM ASIV or 11 µM SP600125. Flow cytometric analysis revealed that 0.1 mM ASIV or 11 µM SP600125 increased the intracellular accumulation of fluorescent P‑gp substrates, including rhodamine 123. Taken together, these results indicated that ASIV reversed the drug resistance of Bel‑7402/FU cells by downregulating the expression of mdr1 via inhibition of the JNK/c‑Jun/AP‑1 signaling pathway.
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http://dx.doi.org/10.3892/mmr.2017.6924DOI Listing
September 2017

[Immunomodulatory effects of human amniotic versus bone marrow-derived mesenchymal stem cells on peripheral blood T lymphocytes in vitro].

Nan Fang Yi Ke Da Xue Xue Bao 2017 Jun;37(6):780-785

Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:

Objective: To compare the immunomodulatory effects of human amniotic mesenchymal stem cell (hAMSCs) and human bone marrow mesenchymal stem cells (hBMSCs) on peripheral blood T lymphocytes in an in vitro co-culture system.

Methods: hAMSCs and hBMSCs isolated using enzymatic digestion and Ficoll-Hypaque density gradient centrifugation, respectively, were culture-expanded in vitro to obtain the 4th-generation cells. The two MSCs were co-cultured separately with human peripheral blood mononuclear cells stimulated with phytohemagglutinin (PHA-PBMSC) to investigate the changes in T lymphocyte subsets using flow cytomety and the production of interleukin-2 (IL-2) and IL-10 by the T lymphocytes using enzyme-linked immunosorbent assay (ELISA).

Results: Co-culture with either hAMSCs or hBMSCs significantly increased the proportions of Treg, Th2 and Tc2 and decreased Th1 and Tc1 cell subsets in the PBMCs as compared with the PBMCs cultured alone (P<0.05), and the changes in the PBMCs were similar between the two co-culture systems (P>0.05). In both of the two co-culture systems, IL-2 production by the lymphocytes was significantly lowered (P<0.05) and IL-10 production was significantly increased (P<0.05) as compared with their levels in the PBMCs cultured alone; no significant difference was found in IL-2 or IL-10 levels between the two co-culture systems (P>0.05).

Conclusion: The MSCs derived from human amnion and bone marrow have similar immunomodulatory effects on the T lymphocytes, suggesting the possibility of using hAMSCs in the treatment of graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744138PMC
June 2017

New chlorinated xanthone and anthraquinone produced by a mangrove-derived fungus Penicillium citrinum HL-5126.

J Antibiot (Tokyo) 2017 Jul 3;70(7):823-827. Epub 2017 May 3.

Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, China.

Two new chlorinated metabolites 4-chloro-1-hydroxy-3-methoxy-6-methyl-8-methoxycarbonyl-xanthen-9-one (1) and 2'-acetoxy-7-chlorocitreorosein (2), together with three known compounds (3-5), were obtained from the EtOAc extract of the endophytic fungus Penicillium citrinum HL-5126 isolated from the mangrove Bruguiera sexangula var. rhynchopetala collected in the South China Sea. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. All compounds were evaluated for their antibacterial and topoisomerase I inhibitory activities. Compound 2 exhibited antibacterial activity against Vibrio parahaemolyticus with an MIC value of 10 μm.
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http://dx.doi.org/10.1038/ja.2017.52DOI Listing
July 2017

Polyphenols Isolated from Xanthoceras sorbifolia Husks and Their Anti-Tumor and Radical-Scavenging Activities.

Molecules 2016 Dec 9;21(12). Epub 2016 Dec 9.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

Bunge. is used in traditional medicine in North China. To evaluate the anti-tumor and radical-scavenging activities of husks polyphenols and determine their structure-activity relationships, 37 polyphenols - were obtained by bioassay-guided fractionation. Two new compounds -, and compounds , , , , , -, -, -, , , , , and were isolated from the genus for the first time. Compounds - did not show strong cytotoxicity against the four tested tumor cell lines (A549, HepG2, MGC-803, and MFC) compared to paclitaxel and under the conditions tested in the anti-tumor assay, but compounds , , , , , -, , , , , , and exhibited stronger radical-scavenging activity than ascorbic acid in a 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay. This was the first report on the anti-tumor and radical-scavenging activities of the polyphenols isolated from husks. Overall, the present study contributed valuable information concerning husks use in medicine and pharmacology.
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http://dx.doi.org/10.3390/molecules21121694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274062PMC
December 2016

[Impact of premature rupture of membranes on neonatal complications in preterm infants with gestational age <37 weeks].

Nan Fang Yi Ke Da Xue Xue Bao 2016 Jun;36(7):887-91

Department of Very Preterm NICU, BaYi Children's Hospital Affiliated to Clinical Medical College in Army General Hospital Of Southern Medical University, Beijing, 100700, China.E-mail:

Objective: To investigate the effect of premature rupture of the membrane (PROM) on neonatal complications in premature infants.

Methods: The registration information of 7684 preterm infants with gestational age <37 weeks were collected from the cooperative units in the task group between January 1, 2014 to December 31, 2014. Specially trained personnel from each cooperative units filled in the unified form in a standardized format to record the gender, gestational age, birth weight, PROM, placental abruption, antenatal corticosteroid, Apgar score, amniotic fluid pollution, and complications of the infants. The data were analyzed comparatively between the cases with PROM and those without (control).

Results: The preterm mortality rate was significantly lower but the incidences of ICH, NEC, ROP and BPD were significantly higher in PROM group than in the control group (P<0.05). The 95% confidence interval of the OR value was <1 for mortality, and was >1 for ICH, NEC, ROP and BPD. After adjustment for gestational age, birth weight, gender, mode of delivery, placental abruption, placenta previa, prenatal hormones, gestational diabetes mellitus (GDM), gestational period hypertension and 5-min Apgar score <7, the incidences of NEC, ROP and BPD were significantly different between the two groups (P<0.05) with 95% confidence interval of OR value >1, but the mortality rate and incidence of ICH were not significantly different between the two groups (P>0.05).

Conclusion: PROM is a risk factor for NEC, ROP and BPD in preterm infants, and adequate intervention of PROM can reduce the incidences of such complications as NEC, ROP and BPD in the infants.
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June 2016

[Identification of Planting Area and Varieties of Rapeseeds by Infrared Spectroscopy Combing with Stepwise Discriminatory Analysis].

Guang Pu Xue Yu Guang Pu Fen Xi 2016 May;36(5):1363-8

The planting area and varieties of rapeseeds were studied based on Fourier transform infrared spectroscopy (FTIR) combining with stepwise discriminatory analysis. The FTIR of 188 rapeseed skin samples of 17 varieties from 5 planting areas was obtained and processed by OMINC 8.0 software. In order to distinguish their planting area and variety, 5 spectra in the range of 1 800~950 cm-1 for each variety from each producing area were selected as training samples arbitrarily with Fisher linear discriminatory criterion which was used to build model by means of stepwise discriminatory analysis by SPSS20.0 software. All the five discriminatory models based on Wilks’Lambda, Unexplained variance, Mahalanobis distance, Smallest F value and Rao’s v can distinguish variety and planting area well, and the best one for identifying variety was on the base of Wilks’Lambda, which yielded correct rate of 97.9%, and the best one for distinguishing planting area was on the base of Unexplained variance with a correct rate of 98.4%. The results showed that discriminatory analysis based on the infrared spectrum of rapeseed skin is an efficient method for identification of rapeseed variety and planting area.
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May 2016

[Synthesis and Study on Adsorption Property of Congo Red Molecularly Imprinted Polymer Nanospheres].

Huan Jing Ke Xue 2015 Jul;36(7):2564-72

Molecularly imprinted polymer nanospheres (MIP) were prepared with Congo red as the template, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross linker, azodiisobutyronitrile (AIBN) as an initiator, and acetonitrile as the porogen by precipitation polymerization. The morphology of MIP was characterized by SEM and TEM which showed that the diameter of MIP was nanometer grade (90 nm) and the shape was homogeneous. The specific surface area and pore volumes of MIP and NIP were examined through Brunauer-Emett-Teller method of nitrogen adsorption experiments. Then, the adsorption and selective recognition ability of MIPs were evaluated using the equilibrium rebinding experiments. The results indicated that the prepared MIP showed a good selectivity recognition ability to its template. It concluded that MIP could be employed as an effective material for removing Congo red from waste water.
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July 2015

Thrombolysis for acute intermediate-risk pulmonary embolism: A meta-analysis.

Thromb Res 2015 Nov 12;136(5):932-7. Epub 2015 Sep 12.

Department of Cardiology, China-Japan Union Hospital of Jilin University, Changchun, China. Electronic address:

Background: The use of thrombolytic therapy in patients with intermediate-risk pulmonary embolism is controversial. To compare with anticoagulation alone, no analysis before has determined whether thrombolytic therapy is associated with improved survival or lower incidence of adverse clinical outcomes for intermediate-risk pulmonary embolism.

Objective: This meta-analysis was performed to assess mortality benefits, bleeding and recurrent pulmonary embolism risks associated with thrombolytic therapy compared with anticoagulation in patients with intermediate-risk pulmonary embolism.

Methods: The Web of Science, PubMed, Embase, EBSCO, and the Cochrane Library databases were searched for randomized clinical trials comparing thrombolytic therapy with anticoagulation in intermediate-risk pulmonary embolism patients (in which the mortality data were reported) from inception to August 5, 2014. Primary outcomes were all-cause mortality and major bleeding. Secondary outcomes were recurrent pulmonary embolism and minor bleeding. The pooled relative risk (RR), Mantel-Haenszel corresponding method and fixed-effect model were used to estimate the efficacy and safety of thrombolytic therapy with 95% confidence intervals.

Results: Eight clinical randomized controlled trials involving 1755 patients with intermediate-risk pulmonary embolism were included. Patients treated with thrombolytics presented lower mortality than patients in the anticoagulation cohort (RR, 0.52; 95% CI, 0.28-0.97; 1.39% [12/866] vs. 2.92% [26/889]). Compared with anticoagulation, thrombolytic therapy was associated with a higher risk of major (RR, 3.35; 95% CI, 2.03-5.54; 7.80% [64/820] vs. 2.28% [19/834]) and minor (RR, 3.66; 95% CI, 2.77-4.84; 32.78% [197/601] vs. 8.94% [53/593]) bleeding. Furthermore, thrombolytic therapy was associated with a lower incidence of recurrent pulmonary embolism (RR, 0.33; 95% CI, 0.15-0.73; 0.73% [6/826] vs. 2.72% [23/846]).

Conclusion: Compared with anticoagulation, thrombolytic therapy in patients with intermediate-risk pulmonary embolism is associated with lower all-cause mortality and recurrent pulmonary embolism risk despite increased major and minor bleeding risks.
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http://dx.doi.org/10.1016/j.thromres.2015.09.012DOI Listing
November 2015

Bioinformatics Analysis of Potential Candidates for Therapy of TDRD7 Deficiency-Induced Congenital Cataract.

Ophthalmic Res 2015 21;54(1):10-7. Epub 2015 May 21.

Department of Ophthalmology, Air Force General Hospital of PLA, Beijing, China.

Aims: The aim of this study was to identify potential candidates and explore the possible mechanism in congenital cataract induced by tudor domain-containing 7 (TDRD7) deficiency.

Methods: The gene expression profile GSE25812 generated from 18 samples was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between disease and normal groups were identified. Then, gene ontology and pathway enrichment analysis of DEGs were performed. The protein-protein interaction (PPI) network and transcription factor (TF) regulatory network were constructed. The modules in the PPI network were identified. Significant target genes were selected from the TF regulatory network.

Results: A total of 329 DEGs were obtained, and downregulated DEGs were significantly enriched in biological processes including defense response and immune response. In the PPI network, high-degree genes of complement component 1, q subcomponent, A/B/C chain (C1QA/C1QB/C1QC), lymphocyte antigen 86 (LY86) and neuroblastoma RAS viral oncogene homolog (NRAS) were identified. From the TF regulatory network, the heat shock 27 kDa protein 1 (HSPB1) was the target of the estrogen receptor 1, and LY86 was the target of the v-myc avian myelocytomatosis viral oncogene homolog.

Conclusion: HSPB1, NRAS, immune response, defense response and the related genes LY86, C1QA/C1QB/C1QC may play an important role in the development of congenital cataract induced by TDRD7 deficiency. However, further experiments are still needed.
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http://dx.doi.org/10.1159/000381478DOI Listing
March 2016