Publications by authors named "Chun Li"

1,805 Publications

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Effect of epigallocatechin gallate on the fermentative and physicochemical properties of fermented milk.

J Dairy Sci 2022 Aug 5. Epub 2022 Aug 5.

Key Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

Yogurt, a traditional fermented dairy product, is made with a starter that contains Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus. The aim of this study was to investigate the effects of different concentrations of epigallocatechin gallate (EGCG; 0, 0.5, 1.0, 3.0, and 5.0 mg/mL) on the growth, metabolism, and acid production of lactic acid bacteria, as well as the texture, stability, and antioxidant activity of fermented milk (yogurt). The results showed that a low concentration of EGCG had no significant effect on the acid production capacity of the starter or on the water-holding capacity of the yogurt but did increase its viscosity. A high concentration (5.0 mg/mL) of EGCG delayed the acid production rate of the starter and decreased the water-holding capacity, but significantly increased the antioxidant activity of yogurt. The addition of EGCG significantly increased the hardness of yogurt. Therefore, EGCG can improve the texture of fermented milk and enhance its antioxidant activity and stability, thus improving the overall quality of yogurt.
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http://dx.doi.org/10.3168/jds.2021-21709DOI Listing
August 2022

Elevated CTSL Gene Expression Correlated with Proinflammatory Cytokines in Omental Adipose Tissue of Patients with Obesity.

Diabetes Metab Syndr Obes 2022 30;15:2277-2285. Epub 2022 Jul 30.

School of Public Health, Kunming Medical University, Kunming, People's Republic of China.

Purpose: Cathepsin L (CTSL) and B (CTSB) were lysosomal proteases, and their expression and activity contribute to the progression of inflammation in obese rodents. Our aim was to investigate CTSB and CTSL expression in omental adipose tissue (AT) of patients with obesity and to correlate CTSB and CTSL expression with proinflammatory cytokines (CCL-2, IL-6 and IL-1β).

Patients And Methods: A total of 12 patients without obesity (NOB) and 51 patients with obesity (OB) were involved in this study. Omental AT was collected from all the participants for RNA extraction. Expressions of CTSB, CTSL and proinflammatory cytokines (CCL-2, IL-6 and IL-1β) were qualified with qRT-PCR. BMI (body mass index) and metabolic parameters were measured.

Results: The mRNA expression levels of both CTSB and CTSL were upregulated in the OB group (t = 2.693, P < 0.05; t = 2.849, <0.01) and were related to TC levels (Std.β=0.443, <0.05; Std.β=0.439, <0.05). However, only the CTSB level was related to BMI (Std.β=0.261, <0.05). In multiple regression analysis, CTSL was independently associated with CCL-2, IL-6 and IL-1β levels (Std.β=0.352-0.462, <0.05).

Conclusion: CTSB and CTSL gene expressions were elevated in the omental AT of OB group. CTSL, but not CTSB, was positively correlated with proinflammatory cytokines independently, suggesting that the dysregulation of CTSL may play a significant role in the inflammatory process.
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http://dx.doi.org/10.2147/DMSO.S373203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348135PMC
July 2022

Superelastic graphene aerogel-based metamaterials.

Nat Commun 2022 Aug 5;13(1):4561. Epub 2022 Aug 5.

Key Laboratory of Organic Optoelectronics & Molecular Engineering, Ministry of Education, Department of Chemistry, Tsinghua University, 100084, Beijing, P. R. China.

Ultralight, ultrastrong, and supertough graphene aerogel metamaterials combining with multi-functionalities are promising for future military and domestic applications. However, the unsatisfactory mechanical performances and lack of the multiscale structural regulation still impede the development of graphene aerogels. Herein, we demonstrate a laser-engraving strategy toward graphene meta-aerogels (GmAs) with unusual characters. As the prerequisite, the nanofiber-reinforced networks convert the graphene walls' deformation from the microscopic buckling to the bulk deformation during the compression process, ensuring the highly elastic, robust, and stiff nature. Accordingly, laser-engraving enables arbitrary regulation on the macro-configurations of GmAs with rich geometries and appealing characteristics such as large stretchability of 5400% reversible elongation, ultralight specific weight as small as 0.1 mg cm, and ultrawide Poisson's ratio range from -0.95 to 1.64. Additionally, incorporating specific components into the pre-designed meta-structures could further achieve diversified functionalities.
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http://dx.doi.org/10.1038/s41467-022-32200-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355988PMC
August 2022

Ultratough Hydrogen-Bond Bridged Phosphorene Films.

Adv Mater 2022 Aug 5:e2203332. Epub 2022 Aug 5.

Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, CB3 0FS, UK.

The rapid development of flexible electronic devices, especially based on two-dimensional(2D) materials, has triggered the demand for high-strength materials. Mono- or few-layer phosphorene with excellent electronic properties has attracted extensive attention. However, phosphorene is affected by its low Young's modulus when applied to flexible electronic devices. Here, we report a strategy via ion intercalation to significantly improve the mechanical properties of black phosphorus to generate hydrogen-bond bridged phosphorene films (HBPFs) with Youngs's modulus as high as 316 GPa. This value is several times larger than the theoretical values of 166 GPa in the zigzag direction, 44 GPa in the armchair direction, and the averaged Young's modulus among all directions of 94 GPa. The impact of intercalation on mechanical properties was also explored. Experimental nanoindentation results obtained by atomic force microscopy indicate that the relationship between the ratio of intercalated ions to phosphorus atoms and the corresponding Young's modulus satisfies the formula . Furthermore, a flexible NO gas sensor device based on this ultratough material presents excellent performance, even after 10000 bending cycles. Our results provide new insight into the potential for practical applications of black phosphorus devices. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202203332DOI Listing
August 2022

Peptidoglycan recognition protein 6 (PGRP6) from Asian corn borer, Ostrinia furnacalis (Guenée) serve as a pattern recognition receptor in innate immune response.

Arch Insect Biochem Physiol 2022 Aug 4:e21955. Epub 2022 Aug 4.

Jiangsu Key Laboratory of Sericutural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Peptidoglycan recognition proteins (PGRPs) recognize invading microbes via detecting peptidoglycans from microbial cell walls. PGRPs are highly conserved from insects to vertebrates and all play roles during the immune defensive response. Ten putative PGRPs have been identified through transcriptome analysis in the Asian corn borer, Ostrinia furnacalis (Guenée). Whereas, the biochemical functions of most of them have not yet been elucidated. In this study, we found PGRP6 messenger RNA exhibited extremely high expression levels in the midgut, and its transcript level increased dramatically upon bacterial infection. Moreover, the enzyme-linked immunosorbent assay indicated recombinant PGRP6 exhibited a strong binding affinity to peptidoglycans from Micrococcus luteus and Bacillus subtilis, which could agglutinate M. luteus and yeast Pichia pastoris. Additionally, we demonstrated that PGRP6 was involved in the pathway of antimicrobial peptides synthesis, but could not enhance encapsulation and melanization of hemocytes. Overall, our results indicated that O. furnacalis PGRP6 serves as a pattern recognition receptor and detects peptidoglycans from microbes to initiate the immune response.
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http://dx.doi.org/10.1002/arch.21955DOI Listing
August 2022

Macrophage Polarization, Metabolic Reprogramming, and Inflammatory Effects in Ischemic Heart Disease.

Front Immunol 2022 18;13:934040. Epub 2022 Jul 18.

Beijing Key Laboratory of Traditional Chinese Medicine (TCM) Syndrome and Formula, Beijing University of Chinese Medicine, Beijing, China.

Macrophages are highly plastic cells, and the polarization-activating actions that represent their functional focus are closely related to metabolic reprogramming. The metabolic reprogramming of macrophages manifests itself as a bias toward energy utilization, transforming their inflammatory phenotype by changing how they use energy. Metabolic reprogramming effects crosstalk with the biological processes of inflammatory action and are key to the inflammatory function of macrophages. In ischemic heart disease, phenotypic polarization and metabolic shifts in circulating recruitment and tissue-resident macrophages can influence the balance of inflammatory effects in the heart and determine disease regression and prognosis. In this review, we present the intrinsic link between macrophage polarization and metabolic reprogramming, discussing the factors that regulate macrophages in the inflammatory effects of ischemic heart disease. Our aim is to estabilsh reliable regulatory pathways that will allow us to better target the macrophage metabolic reprogramming process and improve the symptoms of ischemic heart disease.
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http://dx.doi.org/10.3389/fimmu.2022.934040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339672PMC
August 2022

Catalytic -methyl amidation of carboxylic acids under cooperative conditions.

RSC Adv 2022 Jul 15;12(32):20550-20554. Epub 2022 Jul 15.

State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmacy, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guizhou Medical University 550014 Guiyang P. R. China

Amide is a fundamental group that is present in molecular structures of all domains of organic chemistry and the construction of this motif with high atom economy is the focus of the current research. Specifically, -methyl amides are valuable building blocks in natural products and pharmaceutical science. Due to the volatile nature of methyl amine, the generation of -methyl amides using simple acids with high atom economy is rare. Herein, we disclose an atom economic protocol to prepare this valuable motif under DABCO/FeO cooperative catalysis. This protocol is operationally simple and compatible with a range of aliphatic and (hetero)aromatic acids with very good yields (60-99%). Moreover, the FeO can be easily recovered and high efficiency is maintained for up to ten cycles.
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http://dx.doi.org/10.1039/d2ra03255dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284536PMC
July 2022

Lamivudine remedies alcoholism by activating acetaldehyde dehydrogenase.

Biochem Pharmacol 2022 Jul 30;203:115199. Epub 2022 Jul 30.

School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, PR China. Electronic address:

Acute ethanol intoxication has become an alarming health problem. In the present study, we discover the beneficial effect of lamivudine on alcoholism in mice. Our results indicate that lamivudine decreases serum alcohol concentration dramatically, and potently activates acetaldehyde dehydrogenase (ALDH) to accelerate the conversion of acetaldehyde to acetic acid, which is finally metabolized by tricarboxylic acid cycle to be CO and HO. Also, lamivudine significantly improves symptoms post drinking, such as prolonging alcohol tolerance time and shortening sobering time, as well as reducing the death rate. This work will provide new strategies for the prevention and treatment of acute alcoholism.
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http://dx.doi.org/10.1016/j.bcp.2022.115199DOI Listing
July 2022

Highly effective liquid and solid phase extraction methods to concentrate radioiodine isotopes for radioiodination chemistry.

J Labelled Comp Radiopharm 2022 Jul 29. Epub 2022 Jul 29.

School of Biomedical Engineering and Imaging Sciences, St. Thomas' Hospital, King's College London, London, UK.

Radioactive iodine isotopes play a pivotal role in radiopharmaceuticals. Large-scale production of multi-patient dose of radioiodinated nuclear medicines requires high concentration of radioiodine. We demonstrate that tetrabutylammonium chloride and methyltrioctylamonium chloride are effective phase transfer reagents to concentrate iodide-124, iodide-125 and iodide-131 from the corresponding commercial water solutions. The resulting concentrated radioiodide, in the presence of either phase transfer reagent, does not hamper the chemical reactivity of aqueous radioiodide in the copper (II)-mediated one-pot three-component click chemistry to produce radioiodinated iodotriazoles.
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http://dx.doi.org/10.1002/jlcr.3994DOI Listing
July 2022

Antiphospholipid Syndrome-Related Pulmonary Embolism: Clinical Characteristics and Early Recognition.

Front Cardiovasc Med 2022 11;9:872523. Epub 2022 Jul 11.

Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, China.

Background: Pulmonary thromboembolism is a common disease frequently encountered in the emergency room and has a high mortality rate. Antiphospholipid syndrome (APS) is a high-risk factor for recurrent pulmonary embolism (PE). It is critical to effectively administer anticoagulants to avoid the recurrence of thrombotic events. This study aims to identify the clinical characteristics of APS patients with PE (APS-PE) and to develop a risk score for determining the presence of APS in PE patients in the emergency situations.

Methods: We retrospectively enrolled 76 PE patients in this study, with 46 patients in the APS-PE group and 30 patients in the non-APS-PE group. We compared differences in demographics, laboratory parameters, and early mortality risk between the two groups. Risk factors for APS-PE were screened using logistic regression analysis. We also developed an early risk score using multivariate analysis weighted points proportional to the β regression coefficient values and calculated the sensitivity and specificity for APS in PE patients.

Results: In the APS-PE group, we observed a higher proportion of males (43.6 vs. 20%), a higher proportion of low-risk patients (58.7 vs. 10%), lower levels of white blood cells and platelets (PLT), longer activated partial thromboplastin time (APTT), and a slight increase in D-dimer levels. Patients who were triple positive for antiphospholipid antibodies (aPLs) were younger. The APTT gradually increased as the number of positive aPLs increased. The risk factors for APS included male (OR = 5.565, 95% CI 1.176-26.341), decreased PLT (OR = 0.029, 95% CI 0.003-0.330), slightly increased D-dimer (OR = 0.089, 95% CI 0.019-0.426), and prolonged APTT (OR = 4.870, 95% CI 1.189-19.951). The risk score was named MPDA and included male, PLT, D-dimer and APTT, which can predict APS in PE patients with the AUC at 0.888 (95% CI 0.811-0.965).

Conclusion: The risk factors for APS in PE patients are male, low PLT, prolonged APTT and slightly increased D-dimer. The MPDA is a quantitative scoring system which is highly suggestive of APS in PE patients.
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http://dx.doi.org/10.3389/fcvm.2022.872523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309373PMC
July 2022

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS.

Metabolites 2022 Jul 26;12(8). Epub 2022 Jul 26.

College of Tropical Crops, Hainan University, Haikou 570228, China.

Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles and non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS and UHPLC-MS/MS in two coconut varieties (Hainan Tall, HT and Green Dwarf, GD). Four metabolite classes of volatiles were associated with good aroma including hydrocarbons, benzenoids, alcohols and esters, and these volatiles were generally higher in GD, especially at 7 and 9 months of coconut growth. Pathway-based metabolomics revealed that flavonols and their derivatives were significantly enriched in HT, and some of these metabolites were key determinants of HT flesh bitterness, including kaempferol 7--glucoside, a known bitter metabolite. Despite the overall accumulation of amino acids, including L-alanine, L-serine and L-methionine in GD, comparative metabolomics revealed that HT flesh provides a higher content of vitamins than GD. This study sheds light on the metabolic pathways and key metabolites differentiating the flesh flavor quality and nutritional value among coconut varieties, and reveals the possible mechanisms of flavor formation and regulation in coconut fruits.
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http://dx.doi.org/10.3390/metabo12080691DOI Listing
July 2022

Controllable Valley Polarization and Strain Modulation in 2D 2H-VS/CuInPSe Heterostructures.

Nanomaterials (Basel) 2022 Jul 18;12(14). Epub 2022 Jul 18.

School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710072, China.

Two-dimensional (2D) transition metal dichalcogenides endow individually addressable valleys in momentum space at the K and K' points in the first Brillouin zone due to the breaking of inversion symmetry and the effect of spin-orbit coupling. However, the application of 2H-VS monolayer in valleytronics is limited due to the valence band maximum (VBM) located at the Γ point. Here, by involving the 2D ferroelectric (FE) CuInPSe (CIPSe), the ferrovalley polarization, electronic structure, and magnetic properties of 2D 2H-VS/CIPSe heterostructures with different stacking patterns and FE polarizations have been investigated by using first-principles calculations. It is found that, for the energetically favorable AB-stacking pattern, the valley polarization is preserved when the FE polarization of CIPSe is upwards (CIPSe↑) or downwards (CIPSe↓) with the splitting energies slightly larger or smaller compared with that of the pure 2H-VS. It is intriguing that, for the FE CIPSe↑ case, the VBM is expected to pass through the Fermi energy level, which can be eventually achieved by applying biaxial strain and thus the valleytronic nature is turned off; however, for the CIPSe↓ situation, the heterostructure basically remains semiconducting even under biaxial strains. Therefore, with the influence of proper strains, the FE polar reversal of CIPSe can be used as a switchable on/off to regulate the valley polarization in VS. These results not only demonstrate that 2H-VS/CIPSe heterostructures are promising potential candidates in valleytronics, but also shed some light on developing practical applications of valleytronic technology.
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http://dx.doi.org/10.3390/nano12142461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315968PMC
July 2022

Antimony Nanoparticles Encapsulated in Self-Supported Organic Carbon with a Polymer Network for High-Performance Lithium-Ion Batteries Anode.

Nanomaterials (Basel) 2022 Jul 6;12(14). Epub 2022 Jul 6.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, CAS, Changchun 130022, China.

Antimony (Sb) demonstrates ascendant reactive activation with lithium ions thanks to its distinctive puckered layer structure. Compared with graphite, Sb can reach a considerable theoretical specific capacity of 660 mAh g by constituting LiSb safer reaction potential. Hereupon, with a self-supported organic carbon as a three-dimensional polymer network structure, Sb/carbon (3DPNS-Sb/C) composites were produced through a hydrothermal reaction channel followed by a heat disposal operation. The unique structure shows uniformitarian Sb nanoparticles wrapped in a self-supported organic carbon, alleviating the volume extension of innermost Sb alloying, and conducive to the integrality of the construction. When used as anodes for lithium-ion batteries (LIBs), 3DPNS-Sb/C exhibits a high invertible specific capacity of 511.5 mAh g at a current density of 0.5 A g after 100 cycles and a remarkable rate property of 289.5 mAh g at a current density of 10 A g. As anodes, LIBs demonstrate exceptional electrochemical performance.
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http://dx.doi.org/10.3390/nano12142322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316927PMC
July 2022

Risk Factors and Outcomes of Acute Myocardial Infarction in a Cohort of Antiphospholipid Syndrome.

Front Cardiovasc Med 2022 5;9:871011. Epub 2022 Jul 5.

Beijing Key Laboratory for Rheumatism and Immune Diagnosis (BZ0135), Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, China.

Background: Antiphospholipid syndrome (APS) is a disorder associated with thromboembolic diseases, including acute myocardial infarction (AMI). Given that AMI is a relatively common condition with poor prognostic features, identification of risk factors for AMI in APS is important.

Methods: A retrospective cohort study was performed consisting of 332 patients with APS, and 239 patients with thrombotic APS were finally included. Patients were followed up in the outpatient department for 5 years. Clinical data and laboratory parameters were analyzed to identify the risk factors for AMI in APS. The primary and secondary clinical outcomes were all-cause mortality and recurrence of thrombosis, respectively.

Results: AMI was observed in 12.1% (29/239) of patients with APS. Compared to patients without AMI, patients with AMI had multiple organ thrombosis (55.1 vs. 34.3%, = 0.029), recurrent thrombosis (58.6 vs. 34.3%, = 0.011), a higher incidence of atherosclerosis (62.1 vs. 23.8%, < 0.001), higher neutrophil count (×10/L) [4.68 (3.25, 8.17) vs. 3.71 (2.64, 5.80), = 0.036], longer QT interval (ms) [438 ms (423, 454) vs. 425 ms (410, 446), = 0.016], and fewer venous thrombosis events (27.6 vs. 63.3%, < 0.001). Multivariate logistic regression analysis (adjusted for age and gender) identified several factors that were positively associated with AMI, including multiple organ thrombosis [odds ratio (OR) 8.862, 95% confidence interval (CI): 1.817-43.212, = 0.007), atherosclerosis (OR 5.397, 95%CI: 1.943-14.994, = 0.001), and elevated neutrophil count (>6.3 ×10/L) (OR 3.271, 95%CI: 1.268-8.440, = 0.014). The venous thrombosis was negatively associated with AMI (OR 0.106, 95%CI: 0.036-0.314, < 0.001). Kaplan-Meier analysis revealed that the recurrence rates of arterial thrombosis differed significantly between patients with AMI and those without AMI [hazard ratio (HR) = 3.307, = 0.038].

Conclusion: Atherosclerosis, multiple organ thrombosis, an increased number of neutrophils are variables positively associated with AMI in APS, and venous thrombosis had a negative association with AMI. AMI only predicts the subsequent recurrence of arterial thrombosis. These findings suggest that distinct pathophysiological mechanisms may exist and contribute to the development of venous or arterial thrombotic APS.
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http://dx.doi.org/10.3389/fcvm.2022.871011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294316PMC
July 2022

Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis the Short-Chain Fatty Acid Propionate.

Front Immunol 2022 4;13:915218. Epub 2022 Jul 4.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, China.

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a very common urological disorder and has been gradually regarded as an immune-mediated disease. Multiple studies have indicated that the gut microflora plays a pivotal part in immune homeostasis and autoimmune disorder development. However, whether the gut microflora affects the CP/CPPS, and the underlying mechanism behind them remain unclear. Here, we built an experimental autoimmune prostatitis (EAP) mouse model by subcutaneous immunity and identified that its Th17/Treg frequency was imbalanced. Using fecal 16s rRNA sequencing and untargeted/targeted metabolomics, we discovered that the diversity and relative abundance of gut microflora and their metabolites were obviously different between the control and the EAP group. Propionic acid, a kind of short-chain fatty acid (SCFA), was decreased in EAP mice compared to that in controls, and supplementation with propionic acid reduced susceptibility to EAP and corrected the imbalance of Th17/Treg cell differentiation and . Furthermore, SCFA receptor G-protein-coupled receptor 43 and intracellular histone deacetylase 6 regulated by propionic acid in Th17 and Treg cells were also evaluated. Lastly, we observed that fecal transplantation from EAP mice induced the decrease of Treg cell frequency in recipient mice. Our data showed that gut dysbiosis contributed to a Th17/Treg differentiation imbalance in EAP the decrease of metabolite propionic acid and provided valuable immunological groundwork for further intervention in immunologic derangement of CP/CPPS by targeting propionic acid.
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http://dx.doi.org/10.3389/fimmu.2022.915218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289123PMC
July 2022

Syntheses and crystal structures of a nitro-anthracene-isoxazole and its oxidation product.

Acta Crystallogr E Crystallogr Commun 2022 Jul 10;78(Pt 7):703-708. Epub 2022 Jun 10.

Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, MT 59812, USA.

The syntheses and structures of an unexpected by-product from an iodination reaction, namely, ethyl 5-methyl-3-(10-nitro-anthracen-9-yl)isoxazole-4-carb-oxy-l-ate, CHNO, (I), and its oxidation product, ethyl 3-(9-hy-droxy-10-oxo-9,10-di-hydro-anthracen-9-yl)-5-methyl-isoxazole-4-carboxyl-ate, CHNO (V) are described. Compound (I) crystallizes with two mol-ecules in the asymmetric unit in which the dihedral angles between the anthracene fused-ring systems and isoxazole ring mean planes are 88.67 (16) and 85.64 (16)°; both mol-ecules feature a disordered nitro group. In (V), which crystallizes with one mol-ecule in the asymmetric unit, the equivalent dihedral angle between the almost planar anthrone ring system (r.m.s. deviation = 0.029 Å) and the pendant isoxazole ring is 89.65 (5)°. In the crystal of (I), the mol-ecules are linked by weak C-H⋯O inter-actions into a three-dimensional network and in the extended structure of (V), inversion dimers linked by pairwise O-H⋯O hydrogen bonds generate (14) loops.
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http://dx.doi.org/10.1107/S2056989022005710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260358PMC
July 2022

D3K: The Dissimilarity-Density-Dynamic Radius K-means Clustering Algorithm for scRNA-Seq Data.

Front Genet 2022 1;13:912711. Epub 2022 Jul 1.

School of Mathematics and Statistics, Hainan Normal University, Haikou, China.

A single-cell sequencing data set has always been a challenge for clustering because of its high dimension and multi-noise points. The traditional K-means algorithm is not suitable for this type of data. Therefore, this study proposes a Dissimilarity-Density-Dynamic Radius-K-means clustering algorithm. The algorithm adds the dynamic radius parameter to the calculation. It flexibly adjusts the active radius according to the data characteristics, which can eliminate the influence of noise points and optimize the clustering results. At the same time, the algorithm calculates the weight through the dissimilarity density of the data set, the average contrast of candidate clusters, and the dissimilarity of candidate clusters. It obtains a set of high-quality initial center points, which solves the randomness of the K-means algorithm in selecting the center points. Finally, compared with similar algorithms, this algorithm shows a better clustering effect on single-cell data. Each clustering index is higher than other single-cell clustering algorithms, which overcomes the shortcomings of the traditional K-means algorithm.
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http://dx.doi.org/10.3389/fgene.2022.912711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284269PMC
July 2022

Social Creativity and Entrepreneurial Intentions of College Students: Mediated by Career Adaptability and Moderated by Parental Entrepreneurial Background.

Front Psychol 2022 28;13:893351. Epub 2022 Jun 28.

Academy of Art and Design, Guangdong AIB Polytechnic, Guangzhou, China.

Drawing on social cognitive career theory, this study aims to ascertain how social creativity influences college students' entrepreneurial intentions, based on the mediating role of career adaptability and the moderating role of parental entrepreneurial background. A total of 715 college students completed an online survey designed to collect information on these variables. SPSS (version 25.0) was used to test the model. The results indicate that after controlling for gender and individual entrepreneurial experience, college students with a high level of social creativity were likely to have a high level of entrepreneurial intention. Career adaptability partially mediates the association between social creativity and entrepreneurial intention. Moreover, both direct and indirect associations between social creativity and entrepreneurial intention were moderated by parental entrepreneurial background. Specifically, compared with college students whose parents had no entrepreneurial background, the relationships between social creativity and entrepreneurial intention, social creativity and career adaptability, and career adaptability and entrepreneurial intention were stronger among college students whose parents had an entrepreneurial background. The findings help to develop promotion programs that are more suitable for college students' entrepreneurship intentions.
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http://dx.doi.org/10.3389/fpsyg.2022.893351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275665PMC
June 2022

Incidental Findings of Coexisting Metastatic Pancreatic Cancer in Patients With Prostate Cancer on 18F-Prostate-Specific Membrane Antigen PET/CT.

Authors:
Yang Lu Chun Li

Clin Nucl Med 2022 Jul 14. Epub 2022 Jul 14.

Cancer System Imaging, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX.

Abstract: A 79-year-old man with rising prostate-specific antigen of 3.2 ng/mL and diagnosis of metastatic castration-resistant prostate cancer received abiraterone and prednisone for treatment regarding prostate-specific membrane antigen (PSMA)-avid bone lesions on PET/CT. Four months later, a follow-up 18F-DCFPyL PET/CT demonstrated new and increased multifocal PSMA-avid osseous and liver lesions, whereas prostate-specific antigen was stabled at 3.1 ng/mL. Biopsy of liver lesion showed metastasis from a primary pancreatic acinar cell carcinoma. Retrospectively, PSMA-avid pancreatic body lesion was identified on both PSMA PET/CT scans. This case illustrated that any above background PSMA uptake in the pancreas warrants suspicion for malignancy.
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http://dx.doi.org/10.1097/RLU.0000000000004338DOI Listing
July 2022

Rapeseed NAM transcription factor positively regulates leaf senescence via controlling senescence-associated gene expression.

Plant Sci 2022 Jul 8;323:111373. Epub 2022 Jul 8.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China. Electronic address:

Leaf senescence is one of the most visible forms of programmed cell death in plants. It can be a seasonal adaptation in trees or the final stage in crops ensuring efficient translocation of nutrients to seeds. Along with developmental cues, various environmental factors could also trigger the onset of senescence through transcriptional cascades. Rapeseed (Brassica napus L.) is an important oil crop with its yielding affected by significant falling leaves as a result of leaf senescence, compared to many other crops. Therefore, a better understanding of leaf senescence and developing strategies controlling the progress of leaf senescence in rapeseed is necessary for warranting vegetable oil security. Here we functionally characterized the gene BnaNAM encoding No Apical Meristem (NAM) homologue to identify transcriptional regulation of leaf senescence in rapeseed. A combination of transient and stable expression techniques revealed overexpression of BnaNAM induced ROS production and leaf chlorosis. Quantitative evaluation of up-regulated genes in BnaNAM overexpression lines identified genes related to ROS production (RbohD, RbohF), proteases (βVPE, γVPE, SAG12, SAG15), chlorophyll catabolism (PaO, PPH) and nucleic acid degradation (BFN1) as the putative downstream targets. A dual luciferase-based transcriptional activation assay of selected promoters further confirmed BnaNAM mediated transactivation of promoters of the downstream genes. Finally, an electrophoretic mobility shift assay further confirmed direct binding of BnaNAM to promoters of βVPE, γVPE, SAG12, SAG15 and BFN1. Our results therefore demonstrate a novel role of BnaNAM in leaf senescence.
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http://dx.doi.org/10.1016/j.plantsci.2022.111373DOI Listing
July 2022

Association Between Urinary Sodium and Potassium Excretion and Blood Pressure Among Non-Hypertensive Adults - China, 2018-2019.

China CDC Wkly 2022 Jun;4(24):522-526

Division of NCD and Risk Factor Surveillance, National Center for Chronic and Non-communicable Disease Control and Prevention, China CDC, Beijing, China.

What Is Already Known About This Topic?: High sodium and low potassium in 24 h urinary excretion were associated with elevated blood pressure.

What Is Added By This Report?: With increasing body mass index levels, decreasing unit urinary sodium excretion was more effective in reducing systolic and diastolic blood pressure, and increasing unit urinary potassium excretion was more effective in reducing diastolic blood pressure.

What Are The Implications For Public Health Practice?: Reducing sodium and increasing potassium intake was more effective in reducing blood pressure in overweight and obese non-hypertensive adults compared to underweight and normal weight adults.
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http://dx.doi.org/10.46234/ccdcw2022.066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257692PMC
June 2022

Immunoregulatory Therapy Improves Reproductive Outcomes in Elevated Th1/Th2 Women with Embryo Transfer Failure.

Biomed Res Int 2022 26;2022:4990184. Epub 2022 Jun 26.

Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, China.

Objective: Immunological disturbance is one of the crucial factors of implantation failure. Limited data exists evaluating immunoregulatory therapy in patients with implantation failures.

Methods: This is a retrospective cohort study on patients who had failed embryo transfer cycle and had elevated Th1/Th2 cytokine ratios between 1/2019 and 3/2020. Patients were assigned into two groups based on whether they received immunoregulatory treatment during a frozen transfer cycle. The primary outcome was live birth rate. Secondary outcomes included clinical pregnancy, implantation rate, and neonatal outcomes.

Results: Of 71 patients enrolled, 41 patients received immunoregulatory therapy and 30 patients did not. Compared to untreated patients, rate of live birth was significantly elevated in the treated group (41.5% vs. 16.7%, = 0.026). Rate of biochemical pregnancy, implantation, clinical pregnancy, and ongoing pregnancy between two groups were 56.1% vs. 40% ( = 0.18), 36.5% vs. 23.9% ( = 0.15), 51.2% vs. 30% ( = 0.074), and 41.5% vs. 16.7% ( = 0.03), respectively. Although there was no statistical significance, women receiving treatment also had a tendency of lower frequency of pregnancy loss (19.0% vs. 44.4%, = 0.20). No adverse events were found between newborns of the two groups. Immunoregulatory therapy, age, infertility type, ovulation induction protocol, number of oocytes retrieved, artificial cycle embryo transfer, and cleavage transfer were associated with live birth in univariate analysis (all < 0.05). Only immunoregulatory therapy was associated with live birth after adjustment of confounders (OR = 5.02, 95% CI: 1.02-24.8, = 0.048).

Conclusions: Immunoregulatory therapy improves reproductive outcomes in elevated Th1/Th2 cytokine ratio women with embryo transfer failure.
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http://dx.doi.org/10.1155/2022/4990184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251089PMC
July 2022

10-Alkoxy-anthracenyl-isoxazole analogs have sub-micromolar activity against a Glioblastoma multiforme cell line.

Bioorg Med Chem 2022 09 1;69:116911. Epub 2022 Jul 1.

Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, MT 59812, USA. Electronic address:

A series of 10-alkoxy-Anthryl-isoxazole-pyrrole-doubletails (RO-AIMs) were synthesized using a crown ether assisted nucleophilic aromatic substitution followed by a modified Schotten-Baumann reaction. The novel RO-AIMs described here exhibit robust growth inhibition for the human SNB19 CNS glioblastoma cell line, and biphenyl analog 8c had activity in the nanomolar regime, which represents the most efficacious compound in the AIM series to date. Computational modeling for RO-AIMs binding in a ternary complex with c-myc quadruplex DNA and its helicase DHX36 is presented which represents our current working hypothesis.
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http://dx.doi.org/10.1016/j.bmc.2022.116911DOI Listing
September 2022

The Spatial Distribution and Genetic Diversity of the Soybean Cyst Nematode, , in China: It Is Time to Take Measures to Control Soybean Cyst Nematode.

Front Plant Sci 2022 15;13:927773. Epub 2022 Jun 15.

Henan Academy of Crops Molecular Breeding, National Centre for Plant Breeding, Zhengzhou Subcenter of National Soybean Improvement Center, Key Laboratory of Oil Crops in Huang Huaihai Plains, Ministry of Agriculture and Rural Affairs, Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, China.

The continuous evolution and spread of virulent forms of the soybean cyst nematode (SCN) driven by the environment and anthropogenic intervention is a serious threat to the soybean production worldwide, including China. Especially in China, the implemented measures to control SCN are insufficient for sustainable agricultural development yet. We summarized our knowledge about the spread and spatial distribution of SCN in China and the virulence diversity in the main soybean growing areas. To reveal the genetic relatedness and diversity of SCN populations, we re-sequenced 53 SCN genomes from the Huang-Huai Valleys, one of the two main soybean growing areas in China. We identified spreading patterns linked to the local agroecosystems and topographies. Moreover, we disclosed the first evidence for the selection of complex virulence in the field even under low selection pressure in an example from North Shanxi. SCN is present in all soybean growing areas in China but SCN susceptible cultivars are still largely grown indicating that SCN-related damage and financial loss have not received the attention they deserve yet. To prevent increasing yield losses and to improve the acceptance of resistant cultivars by the growers, we emphasized that it is time to accelerate SCN resistance breeding, planting resistant cultivars to a larger extent, and to support farmers to implement a wider crop rotation for sustainable development of the soybean production in China.
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http://dx.doi.org/10.3389/fpls.2022.927773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242501PMC
June 2022

Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice.

Life Sci 2022 Sep 22;304:120725. Epub 2022 Jun 22.

Department of Pharmacy, Lanzhou University, Lanzhou 730000, PR China; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou 730000, PR China. Electronic address:

Aims: Diabetes mellitus (DM) is a major global health threat characterized by insulin resistance. A new tactic to ameliorate insulin resistance, thereby reversing the exacerbation of DM, is urgently needed. The work is aiming to provide a new strategy for DM treatment as well as to identify new targets.

Main Methods: C57BL/6 N mice were raised with high-fat diet (HFD) and infused with streptozotocin (STZ) to induce diabetes. The blood glucose, serum insulin, blood lipid and oxidative stress were detected. In vitro insulin resistance model experiment has been made to examine the molecular mechanisms underlying anti-diabetic effect of potential active chemicals in human hepatocellular carcinoma cells (HepG2).

Key Findings: Acyclovir, an antiviral nucleotide analog, alleviates insulin resistance by reducing blood lipids as well as oxidative stress and elevating insulin sensitivity on diabetic mice, which is in accord with results in the insulin resistance model of HepG2 cells. Mechanically, acyclovir stimulates pyruvate kinase M1 (PKM1) directly to activate adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)/Sirtuin1 (SIRT1) signaling pathway, thus improving insulin resistance.

Significance: The present study supports that acyclovir should be translated to remedy DM, and PKM1 might be a valuable target to develop new medicines.
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http://dx.doi.org/10.1016/j.lfs.2022.120725DOI Listing
September 2022

A novel fully human recombinant antibody neutralizing α-hemolysin of Staphylococcus aureus.

APMIS 2022 Sep 12;130(9):578-589. Epub 2022 Jul 12.

Immune Mechanism and Therapy of Major Diseases of Luzhou Key Laboratory, Public Center of Experimental Technology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, China.

Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to almost all β-lactam antibiotics. Hence, new ways to control MRSA infection, such as antibacterial antibodies, need to be explored. α-hemolysin is the most important virulence factor widely expressed in S. aureus. This study aimed to develop a new fully human antibody against α-hemolysin of S. aureus and research its neutralizing effect. The single-chain antibody fragments (scFvs) against S. aureus were screened from a fully human scFv library using phage display technology. The selected scFvs had good binding affinities to α-hemolysin and S. aureus. The IgG-like scFv-Fc inserted into the pcDNA3.1 or pMH3 vector was expressed in HEK293F suspension cells to extend the half-life and restore Fc function. The size of purified scFv-Fc was about 55 kDa. The functions of expressed scFv-Fcs against α-hemolysin were validated. The cytotoxicity assays showed that scFv555-Fc had better protective effects on A549 cells than other scFv-Fcs. The results of anti-rabbit erythrocyte lysis and A549 cell apoptosis assay confirmed that scFv555-Fc had a significant neutralizing effect on α-hemolysin. The scFv555-Fc was used to construct the docking model of antigen-antibody complexes using Discovery Studio software. It predicted that the key binding sites of α-hemolysin were TYR28, LYS37, PHE39, ARG56, and LYS58, which might be the key toxic sites of α-hemolysin. A novel fully human scFv-Fc antibody neutralizing the α-hemolysin toxin of S. aureus was successfully developed. The findings might provide a new theoretical basis and treatment method for preventing MRSA infection.
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http://dx.doi.org/10.1111/apm.13258DOI Listing
September 2022

Synergistic Anti-Inflammatory Activity of Ginger and Turmeric Extracts in Inhibiting Lipopolysaccharide and Interferon-γ-Induced Proinflammatory Mediators.

Molecules 2022 Jun 16;27(12). Epub 2022 Jun 16.

NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia.

This study aims to investigate the combined anti-inflammatory activity of ginger and turmeric extracts. By comparing the activities of individual and combined extracts in lipopolysaccharide and interferon-γ-induced murine RAW 264.7 cells, we demonstrated that ginger-turmeric combination was optimal at a specific ratio (5:2, /) in inhibiting nitric oxide, tumour necrosis factor and interleukin 6 with synergistic interaction (combination index < 1). The synergistic inhibitory effect on TNF was confirmed in human monocyte THP-1 cells. Ginger-turmeric combination (5:2, /) also upregulated nuclear factor erythroid 2-related factor 2 activity and heme oxygenase-1 protein expression. Additionally, 6-shogaol, 8-shogaol, 10-shogaol and curcumin were the leading compounds in reducing major proinflammatory mediators and cytokines, and a simplified compound combination of 6-s, 10-s and curcumin showed the greatest potency in reducing LPS-induced NO production. Our study provides scientific evidence in support of the combined use of ginger and turmeric to alleviate inflammatory processes.
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http://dx.doi.org/10.3390/molecules27123877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229778PMC
June 2022

Interactions Between Natural Products and Tamoxifen in Breast Cancer: A Comprehensive Literature Review.

Front Pharmacol 2022 2;13:847113. Epub 2022 Jun 2.

NICM Health Research Institute, Western Sydney University, Westmead, NSW, Australia.

Tamoxifen (TAM) is the most commonly used hormone therapeutic drug for the treatment of estrogen receptor-positive (ER+) breast cancer. 30%-70% of clinical breast cancer patients use natural products, which may increase the likelihood of drug interactions. To evaluate the evidence for the interactions between natural products and TAM in breast cancer. Electronic databases, including PubMed, CINAHL Plus ( EbscoHost), European PMC, Medline, and Google Scholar, were searched for relevant publications. The search terms include complementary and alternative medicine, natural products, plant products, herbs, interactions, tamoxifen, breast cancer, and their combinations. Various and studies demonstrated that the combined use of natural products with TAM produced synergistic anti-cancer effects, including improved inhibition of tumor cell growth and TAM sensitivity and reduced side effects or toxicity of TAM. In contrast, some natural products, including (Oliv.) Diels [Apiaceae] Pall, (Gaertn.) DC. Bunge, and L. [Fabaceae], showed estrogen-like activity, which may reduce the anti-cancer effect of TAM. Some natural products, including morin, silybin, epigallocatechin gallate (EGCG), myricetin, baicalein, curcumin, kaempferol, or quercetin, were found to increase the bioavailability of TAM and its metabolites . However, three are limited clinical studies on the combination of natural products and TAM. There is evidence for potential interactions of various natural products with TAM in pre-clinical studies, although the relevant clinical evidence is still lacking. Further studies are warranted to evaluate the potential interactions of natural products with TAM in clinical settings.
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http://dx.doi.org/10.3389/fphar.2022.847113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201062PMC
June 2022

[HPLC fingerprint and content determination of three principal components in seeds of Crataegus pinnatifida].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):3000-3006

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

The purpose of this paper is to establish the HPLC fingerprint and content determination method for Crataegus pinnatifida seeds. The separation was developed on a Welch Ultimate XB C_(18) column(4.6 mm×250 mm, 5 μm) by gradient elution with acetonitrile-water containing 0.1% formic acid as the mobile phase at the flow rate of 1 mL·min~(-1), the column temperature of 30 ℃, the injection volume of 10 μL, and the detection wavelength of 320 nm. Eighteen batches of samples were analyzed under the above chromatographic conditions to establish the fingerprint of C. pinnatifida seeds from different producing areas and a total of 24 common peaks were selected. The structures of three main chromatographic peaks were identified by comparison to reference substances, and the three compounds were simultaneously analyzed for content determination. They were identified as erythro-(7S,8R)-guaiacylglycerol-β-coniferyl aldehyde ether, threo-(7R,8R)-guaiacylglycerol-β-coniferyl aldehyde ether, and balanophonin, respectively. The relative similarity of fingerprints of 18 batches of samples and references ranged from 0.928 to 0.999, and the content of the three compounds was 0.055 1-0.182 7, 0.061 8-0.225 8, and 0.156 8-0.405 6 mg·g~(-1), respectively. SPSS 17.0 and SIMCA 14.1 were used for cluster analysis, principal component analysis(PCA), and orthogonal partial least squares discriminant analysis(OPLS-DA) on the common peaks of the HPLC fingerprint of C. pinnatifida seeds. The results showed that there were significant differences between the two batches of samples from Liaoning province and the other samples, and the three compounds to be tested were the main components leading to the difference of C. pinnatifida seeds. The established method was simple and reliable and can be used for qualitative and quantitative analysis of C. pinnatifida seeds. The findings of this study are expected to provide a scientific basis for quality control of C. pinnatifida seeds.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211227.202DOI Listing
June 2022

Long noncoding RNA promotes bone regeneration by maintaining bone marrow mesenchymal stem cells activity.

PeerJ 2022 9;10:e13475. Epub 2022 Jun 9.

Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan, China.

Background: Long noncoding RNA can regulate hypothalamic neural stem cells (htNSCs) senescence and the aging process. However, the effect of on the senescence of bone marrow mesenchymal stem cells (BMSCs) and bone regeneration is unclear. In the present study, we investigated the effects of on the senescence of BMSCs and bone regeneration.

Methods: knockout (-KO) and wild-type (WT) mice were used to establish a bone regeneration model. The Brdu labelling, CCK8 assay, wound healing assay, β-gal staining and osteogenic differentiation assay were used to assess the effects of on the functions of BMSCs. Micro-computed tomography (CT), histochemical and immunohistochemical staining were used to evaluate the ability of bone regeneration. The mimic of , theaflavin 3-gallate, was used to investigate its role on the senescence of BMSCs and bone regeneration.

Results: The expression of reduced in BMSCs of middle-aged mice was compared with that of young mice. The deletion of was sufficient to drive the senescence of BMSCs, resulting in impaired bone regeneration in mice. Mechanistically, could interact with Y-box protein 1(YB-1) and delay its degradation, decreasing the transcription of of BMSCs. We also found that theaflavin 3-gallate could alleviate the senescence of BMSCs and promote bone regeneration in middle-aged mice.

Conclusion: These results indicated that played an important role on BMSCs senescence and bone regeneration and provided a therapeutic target to promote bone regeneration.
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http://dx.doi.org/10.7717/peerj.13475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188769PMC
June 2022
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