Publications by authors named "Christoph Rader"

100Publications

Site-Specific Antibody-Drug Conjugates in Triple Variable Domain Fab Format.

Biomolecules 2020 May 14;10(5). Epub 2020 May 14.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom10050764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278019PMC
May 2020

Chemically Programmable and Switchable CAR-T Therapy.

Angew Chem Int Ed Engl 2020 07 18;59(29):12178-12185. Epub 2020 May 18.

Department of Immunology and Microbiology, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL, 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202005432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429910PMC
July 2020

Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications.

J Biol Chem 2020 May 19;295(18):5995-6006. Epub 2020 Mar 19.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, Florida 33458

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.RA120.012791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196640PMC
May 2020

Dual-mechanistic antibody-drug conjugate site-specific selenocysteine/cysteine conjugation.

Antib Ther 2019 Oct 24;2(4):71-78. Epub 2019 Oct 24.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/abt/tbz009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953743PMC
October 2019

Bispecific antibodies in cancer immunotherapy.

Authors:
Christoph Rader

Curr Opin Biotechnol 2019 Dec 13;65:9-16. Epub 2019 Dec 13.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.copbio.2019.11.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292752PMC
December 2019

Site-Specific Lysine Arylation as an Alternative Bioconjugation Strategy for Chemically Programmed Antibodies and Antibody-Drug Conjugates.

Bioconjug Chem 2019 11 1;30(11):2889-2896. Epub 2019 Nov 1.

Chemical Biology Laboratory, Center for Cancer Research , National Cancer Institute, National Institutes of Health , Frederick , Maryland 21702 , United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.bioconjchem.9b00609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518637PMC
November 2019

Conventional and Chemically Programmed Asymmetric Bispecific Antibodies Targeting Folate Receptor 1.

Front Immunol 2019 21;10:1994. Epub 2019 Aug 21.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2019.01994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712926PMC
October 2020

Six-packed antibodies punch better.

Haematologica 2019 09;104(9):1696-1699

Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3324/haematol.2019.224196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717577PMC
September 2019

Engineering Dual Variable Domains for the Generation of Site-Specific Antibody-Drug Conjugates.

Methods Mol Biol 2019 ;2033:39-52

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-9654-4_4DOI Listing
April 2020

Site-Selective Antibody Functionalization via Orthogonally Reactive Arginine and Lysine Residues.

Cell Chem Biol 2019 09 20;26(9):1229-1239.e9. Epub 2019 Jun 20.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2019.05.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754284PMC
September 2019

Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting.

Cancer Cell 2019 03;35(3):489-503.e8

Program in Immunology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, D3-100, Seattle, WA 98109-1024, USA; Department of Immunology, University of Washington, Seattle, WA 98109, USA; Department of Medicine, University of Washington, Seattle, WA 98109, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ccell.2019.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450658PMC
March 2019

CAR T cells targeting αβ integrin are effective against advanced cancer in preclinical models.

Adv Cell Gene Ther 2018 Sep 10;1(2). Epub 2018 Jul 10.

Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg, Würzburg, Germany.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/acg2.11
Publisher Site
http://dx.doi.org/10.1002/acg2.11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226260PMC
September 2018

Rabbit models of human diseases for diagnostics and therapeutics development.

Dev Comp Immunol 2019 03 16;92:99-104. Epub 2018 Oct 16.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dci.2018.10.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364550PMC
March 2019

A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries.

J Mol Biol 2018 10 11;430(21):4387-4400. Epub 2018 Sep 11.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00222836183104
Publisher Site
http://dx.doi.org/10.1016/j.jmb.2018.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186506PMC
October 2018

An IgG1-like bispecific antibody targeting CD52 and CD20 for the treatment of B-cell malignancies.

Methods 2019 02 24;154:70-76. Epub 2018 Aug 24.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymeth.2018.08.008DOI Listing
February 2019

Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1.

Proc Natl Acad Sci U S A 2018 06 29;115(24):E5467-E5476. Epub 2018 May 29.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458;

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.1719905115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004433PMC
June 2018

Genome Mining of Micromonospora yangpuensis DSM 45577 as a Producer of an Anthraquinone-Fused Enediyne.

Org Lett 2017 11 31;19(22):6192-6195. Epub 2017 Oct 31.

Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, and ∥Department of Immunology and Microbiology, The Scripps Research Institute , Jupiter, Florida 33458, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.7b03120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204215PMC
November 2017

Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates.

Nat Commun 2017 10 24;8(1):1112. Epub 2017 Oct 24.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-017-01257-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653646PMC
October 2017

From rabbit antibody repertoires to rabbit monoclonal antibodies.

Exp Mol Med 2017 03 24;49(3):e305. Epub 2017 Mar 24.

Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/emm.2017.23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382564PMC
March 2017

Stable and Potent Selenomab-Drug Conjugates.

Cell Chem Biol 2017 Apr 16;24(4):433-442.e6. Epub 2017 Mar 16.

Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA; Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2017.02.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400723PMC
April 2017

Utilization of Selenocysteine for Site-Specific Antibody Conjugation.

Methods Mol Biol 2017 ;1575:145-164

Department of Cancer Biology, The Scripps Research Institute, 130 Scripps Way #2C1, Jupiter, FL, 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-6857-2_8DOI Listing
February 2018

Human Serum Albumin Domain I Fusion Protein for Antibody Conjugation.

Bioconjug Chem 2016 10 26;27(10):2271-2275. Epub 2016 Sep 26.

Department of Chemistry, Department of Cell and Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute , La Jolla, California 92037, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.bioconjchem.6b00432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367151PMC
October 2016

Tagging the Untaggable: A Difluoroalkyl-Sulfinate Ketone-Based Reagent for Direct C-H Functionalization of Bioactive Heteroarenes.

Bioconjug Chem 2016 09 15;27(9):1965-71. Epub 2016 Aug 15.

Department of Chemistry, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.bioconjchem.6b00382DOI Listing
September 2016

Chemically Programmed Bispecific Antibodies in Diabody Format.

J Biol Chem 2016 09 21;291(37):19661-73. Epub 2016 Jul 21.

From the Departments of Cancer Biology, Molecular Therapeutics, The Scripps Research Institute, Jupiter, Florida 33458,

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.M116.745588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016699PMC
September 2016

Assessment of reagents for selenocysteine conjugation and the stability of selenocysteine adducts.

Org Biomol Chem 2016 Jun 17;14(22):5141-7. Epub 2016 May 17.

Department of Chemistry, The Scripps Research Institute, Scripps Florida, Jupiter FL 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c6ob00775aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887430PMC
June 2016

Engineered production of cancer targeting peptide (CTP)-containing C-1027 in Streptomyces globisporus and biological evaluation.

Bioorg Med Chem 2016 09 8;24(17):3887-3892. Epub 2016 Apr 8.

Division of Pharmaceutical Sciences, University of Wisconsin, Madison, WI 53705, USA; Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458, USA; Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458, USA; Natural Products Library Initiative at The Scripps Research Institute, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmc.2016.04.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972678PMC
September 2016

Angucyclines and Angucyclinones from Streptomyces sp. CB01913 Featuring C-Ring Cleavage and Expansion.

J Nat Prod 2015 Oct 3;78(10):2471-80. Epub 2015 Sep 3.

Xiangya International Academy of Translational Medicine, Central South University , Changsha, Hunan 410013, People's Republic of China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jnatprod.5b00601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845661PMC
October 2015

IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas.

PLoS One 2015 14;10(7):e0133152. Epub 2015 Jul 14.

Department of Pediatrics, Division of Hematology, Oncology and Stem Cell Transplantation, New York Medical College, Valhalla, NY, United States of America; Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, United States of America; Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, United States of America.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133152PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501840PMC
April 2016

Site-Specific Dual Antibody Conjugation via Engineered Cysteine and Selenocysteine Residues.

Bioconjug Chem 2015 Nov 24;26(11):2243-8. Epub 2015 Jul 24.

Department of Cell and Molecular Biology, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

View Article

Download full-text PDF

Source
https://s3-eu-west-1.amazonaws.com/pstorage-acs-6854636/3740
Web Search
http://pubs.acs.org/doi/10.1021/acs.bioconjchem.5b00244
Publisher Site
http://dx.doi.org/10.1021/acs.bioconjchem.5b00244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839195PMC
November 2015

ROR1-targeted delivery of OSU-2S, a nonimmunosuppressive FTY720 derivative, exerts potent cytotoxicity in mantle-cell lymphoma in vitro and in vivo.

Exp Hematol 2015 Sep 29;43(9):770-4.e2. Epub 2015 Apr 29.

Division of Hematology, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, OH, USA; Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA; Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exphem.2015.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299545PMC
September 2015

Lipid-directed vinculin dimerization.

Biochemistry 2015 May 24;54(17):2758-68. Epub 2015 Apr 24.

†Cell Adhesion Laboratory, ‡Department of Cancer Biology, and §Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, Florida 33458, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biochem.5b00015DOI Listing
May 2015

Chemical biology: How to minimalize antibodies.

Authors:
Christoph Rader

Nature 2015 Feb;518(7537):38-9

Departments of Cancer Biology and Molecular Therapeutics, The Scripps Research Institute, Jupiter, Florida 33458, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/518038aDOI Listing
February 2015

Recognition of antigen-specific B-cell receptors from chronic lymphocytic leukemia patients by synthetic antigen surrogates.

Chem Biol 2014 Dec 26;21(12):1670-9. Epub 2014 Nov 26.

Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458, USA; Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2014.10.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272629PMC
December 2014

Safety of targeting ROR1 in primates with chimeric antigen receptor-modified T cells.

Cancer Immunol Res 2015 Feb 29;3(2):206-16. Epub 2014 Oct 29.

Fred Hutchinson Cancer Research Center, Seattle, Washington. Department of Medicine, University of Washington, Seattle, Washington. Institute for Advanced Study, Technical University of Munich, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/2326-6066.CIR-14-0163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324006PMC
February 2015

Harnessing the fcμ receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia.

Cancer Res 2014 Dec 24;74(24):7510-7520. Epub 2014 Oct 24.

Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/0008-5472.CAN-14-2030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268434PMC
December 2014

The nonsignaling extracellular spacer domain of chimeric antigen receptors is decisive for in vivo antitumor activity.

Cancer Immunol Res 2015 Feb 11;3(2):125-35. Epub 2014 Sep 11.

Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Immunology, Seattle, Washington. Department of Medicine, University of Washington, Seattle, Washington. Technical University of Munich, Institute for Advanced Study, Munich, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/2326-6066.CIR-14-0127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692801PMC
February 2015

Carlos F. Barbas III (1964-2014): Visionary at the interface of chemistry and biology.

ACS Chem Biol 2014 Aug;9(8):1645-6

Department of Cancer Biology and Department of Molecular Therapeutics, The Scripps Research Institute , Scripps Florida, 130 Scripps Way, Jupiter, Florida 33458, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/cb5005993DOI Listing
August 2014

Improving the serum stability of site-specific antibody conjugates with sulfone linkers.

Bioconjug Chem 2014 Aug 6;25(8):1402-7. Epub 2014 Aug 6.

The Skaggs Institute for Chemical Biology, Department of Chemistry, and Department of Cell and Molecular Biology, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bc500276mDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140540PMC
August 2014

Selection of apoptotic cell specific human antibodies from adult bone marrow.

PLoS One 2014 23;9(4):e95999. Epub 2014 Apr 23.

School of Medicine, New York University, New York, New York, United States of America.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095999PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997490PMC
June 2015

Chemically programmed antibodies.

Authors:
Christoph Rader

Trends Biotechnol 2014 Apr 11;32(4):186-97. Epub 2014 Mar 11.

Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, 130 Scripps Way #2C1, Jupiter, FL 33458, USA; Department of Molecular Therapeutics, The Scripps Research Institute, Scripps Florida, 130 Scripps Way #2C1, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tibtech.2014.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3978777PMC
April 2014

Analysis of cell-cell contact mediated by Ig superfamily cell adhesion molecules.

Curr Protoc Cell Biol 2013 Dec 2;61:9.5.1-9.5.85. Epub 2013 Dec 2.

Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/0471143030.cb0905s61DOI Listing
December 2013

Antibody conjugation via one and two C-terminal selenocysteines.

Methods 2014 Jan 10;65(1):133-8. Epub 2013 Jun 10.

Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA; Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; Department of Molecular Therapeutics, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymeth.2013.05.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871924PMC
January 2014

Receptor affinity and extracellular domain modifications affect tumor recognition by ROR1-specific chimeric antigen receptor T cells.

Clin Cancer Res 2013 Jun 25;19(12):3153-64. Epub 2013 Apr 25.

Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-13-0330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804130PMC
June 2013

Application of strain-promoted azide-alkyne cycloaddition and tetrazine ligation to targeted Fc-drug conjugates.

Bioconjug Chem 2012 Oct 25;23(10):2007-13. Epub 2012 Sep 25.

Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bc300052uDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3474869PMC
October 2012

Chemically programmed bispecific antibodies that recruit and activate T cells.

J Biol Chem 2012 Aug 3;287(34):28206-14. Epub 2012 Jul 3.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.M112.384594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436515PMC
August 2012

Cloning, expression, and purification of monoclonal antibodies in scFv-Fc format.

Methods Mol Biol 2012 ;901:209-32

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/978-1-61779-931-0_14
Publisher Site
http://dx.doi.org/10.1007/978-1-61779-931-0_14DOI Listing
October 2012

Selection of human Fab libraries by phage display.

Authors:
Christoph Rader

Methods Mol Biol 2012 ;901:81-99

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-61779-931-0_5DOI Listing
October 2012

Generation of human Fab libraries for phage display.

Authors:
Christoph Rader

Methods Mol Biol 2012 ;901:53-79

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-61779-931-0_4DOI Listing
October 2012

Targeting malignant B cells with an immunotoxin against ROR1.

MAbs 2012 May-Jun;4(3):349-61. Epub 2012 Apr 26.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4161/mabs.19870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3355487PMC
February 2013

Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies.

PLoS One 2011 15;6(6):e21018. Epub 2011 Jun 15.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021018PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115963PMC
November 2011

DARTs take aim at BiTEs.

Authors:
Christoph Rader

Blood 2011 Apr;117(17):4403-4

National Cancer Institute, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood-2011-02-337691DOI Listing
April 2011

The New Zealand black mouse as a model for the development and progression of chronic lymphocytic leukemia.

Cytometry B Clin Cytom 2010 ;78 Suppl 1:S98-109

Department of Pathology and Lab Medicine, University of Medicine and Dentistry/New Jersey Medical School, Newark, New Jersey 07103, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/cyto.b.20544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963456PMC
December 2010

A novel high-affinity human monoclonal antibody to mesothelin.

Int J Cancer 2011 May;128(9):2020-30

Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4264, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/ijc.25557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978266PMC
May 2011

Mining human antibody repertoires.

MAbs 2010 Jul-Aug;2(4):365-78. Epub 2010 Jul 1.

2Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute.

View Article

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180084PMC
http://dx.doi.org/10.4161/mabs.12187DOI Listing
April 2015

Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element.

Proc Natl Acad Sci U S A 2010 Mar 15;107(13):5810-4. Epub 2010 Mar 15.

Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.

View Article

Download full-text PDF

Source
http://www.pnas.org/cgi/doi/10.1073/pnas.0914946107
Publisher Site
http://dx.doi.org/10.1073/pnas.0914946107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851902PMC
March 2010

Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev.

J Mol Biol 2010 Apr 4;397(3):697-708. Epub 2010 Feb 4.

Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892-2775, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmb.2010.01.061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851401PMC
April 2010

Molecularly defined antibody conjugation through a selenocysteine interface.

Biochemistry 2009 Dec;48(50):12047-57

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1203, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/bi901744tDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825887PMC
December 2009

A human monoclonal antibody drug and target discovery platform for B-cell chronic lymphocytic leukemia based on allogeneic hematopoietic stem cell transplantation and phage display.

Blood 2009 Nov 10;114(20):4494-502. Epub 2009 Aug 10.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1203, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood-2009-05-222786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777128PMC
November 2009

Generation and selection of rabbit antibody libraries by phage display.

Authors:
Christoph Rader

Methods Mol Biol 2009 ;525:101-28, xiv

Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/978-1-59745-554-1_5
Publisher Site
http://dx.doi.org/10.1007/978-1-59745-554-1_5DOI Listing
April 2009

Overview on concepts and applications of Fab antibody fragments.

Authors:
Christoph Rader

Curr Protoc Protein Sci 2009 Feb;Chapter 6:Unit 6.9

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/0471140864.ps0609s55
Publisher Site
http://dx.doi.org/10.1002/0471140864.ps0609s55DOI Listing
February 2009

E. coli expression and purification of Fab antibody fragments.

Curr Protoc Protein Sci 2009 Feb;Chapter 6:Unit 6.10

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/0471140864.ps0610s55DOI Listing
February 2009

Application of a trifunctional reactive linker for the construction of antibody-drug hybrid conjugates.

Bioorg Med Chem Lett 2008 Nov 14;18(21):5785-8. Epub 2008 Oct 14.

Laboratory of Medicinal Chemistry, CCR, NCI, NIH, Frederick, MD 21702, USA.

View Article

Download full-text PDF

Source
http://linkinghub.elsevier.com/retrieve/pii/S0960894X0801146
Publisher Site
http://dx.doi.org/10.1016/j.bmcl.2008.09.078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461582PMC
November 2008

Mechanisms of CNS myelin inhibition: evidence for distinct and neuronal cell type specific receptor systems.

Restor Neurol Neurosci 2008 ;26(2-3):97-115

Center for Neural Development and Disorder, Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA.

View Article

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259427PMC
December 2008

Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.

J Mol Biol 2008 Dec 16;384(5):1143-56. Epub 2008 Sep 16.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmb.2008.09.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2659651PMC
December 2008

An engineered selenocysteine defines a unique class of antibody derivatives.

Proc Natl Acad Sci U S A 2008 Aug 21;105(34):12451-6. Epub 2008 Aug 21.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1203, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.0800800105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518826PMC
August 2008

Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.

Clin Cancer Res 2008 Jan;14(2):396-404

Experimental Transplantation and Immunology Branch and Metabolism Branch, Center for Cancer Research, National Cancer Institute, and Hematology Branch, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892-1203, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-07-1823DOI Listing
January 2008

Synthesis of the next-generation therapeutic antibodies that combine cell targeting and antibody-catalyzed prodrug activation.

Proc Natl Acad Sci U S A 2007 Mar 19;104(13):5584-9. Epub 2007 Mar 19.

Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://www.pnas.org/cgi/doi/10.1073/pnas.0700223104
Publisher Site
http://dx.doi.org/10.1073/pnas.0700223104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838444PMC
March 2007

Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector.

J Immunol Methods 2007 Jan 7;318(1-2):75-87. Epub 2006 Nov 7.

Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1203, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jim.2006.10.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1930160PMC
January 2007

Small molecule drug activity in melanoma models may be dramatically enhanced with an antibody effector.

Int J Cancer 2006 Sep;119(5):1194-207

Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/ijc.21924
Publisher Site
http://dx.doi.org/10.1002/ijc.21924DOI Listing
September 2006

Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display.

Biosci Biotechnol Biochem 2005 Dec;69(12):2302-11

School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selanger.

View Article

Download full-text PDF

Source
http://www.tandfonline.com/doi/full/10.1271/bbb.69.2302
Publisher Site
http://dx.doi.org/10.1271/bbb.69.2302DOI Listing
December 2005

Phenotypic knockout of VEGF-R2 and Tie-2 with an intradiabody reduces tumor growth and angiogenesis in vivo.

Proc Natl Acad Sci U S A 2005 Jun 31;102(23):8293-8. Epub 2005 May 31.

Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://www.pnas.org/cgi/doi/10.1073/pnas.0503168102
Publisher Site
http://dx.doi.org/10.1073/pnas.0503168102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1149442PMC
June 2005

Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody.

Cancer Res 2005 Feb;65(3):972-81

Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
February 2005

Chemical adaptor immunotherapy: design, synthesis, and evaluation of novel integrin-targeting devices.

J Med Chem 2004 Nov;47(23):5630-40

The Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/jm049666kDOI Listing
November 2004

Aldolase antibody activation of prodrugs of potent aldehyde-containing cytotoxics for selective chemotherapy.

Chemistry 2004 Oct;10(21):5467-72

Skaggs Institute for Chemical Biology and Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.200400419DOI Listing
October 2004

Isolation of human prostate cancer cell reactive antibodies using phage display technology.

J Immunol Methods 2004 Aug;291(1-2):137-51

The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jim.2004.05.004DOI Listing
August 2004

Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library.

J Immunol Methods 2004 May;288(1-2):149-64

Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jim.2004.03.005DOI Listing
May 2004

New chemical adaptor unit designed to release a drug from a tumor targeting device by enzymatic triggering.

Bioorg Med Chem 2004 Apr;12(8):1853-8

School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel Aviv 69978, Israel.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmc.2004.01.041DOI Listing
April 2004