Chrispin Chaguza, PhD - Wellcome Sanger Institute, Wellcome Genome Campus - Postdoctoral Fellow

Chrispin Chaguza

PhD

Wellcome Sanger Institute, Wellcome Genome Campus

Postdoctoral Fellow

Cambridge | United Kingdom

Main Specialties: Epidemiology

Additional Specialties: Bioinformatics, Genetic Epidemiology, Genomics and Applied Statistics


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Chrispin Chaguza, PhD - Wellcome Sanger Institute, Wellcome Genome Campus - Postdoctoral Fellow

Chrispin Chaguza

PhD

Introduction

I'm a Postdoctoral Fellow at the Wellcome Sanger Institute in Cambridge, UK.

Primary Affiliation: Wellcome Sanger Institute, Wellcome Genome Campus - Cambridge , United Kingdom

Specialties:

Additional Specialties:

Research Interests:

Education

Dec 2017
Institute of Infection and Global Health, University of Liverpool
PhD
Sep 2012
The University of Manchester
MSc
Nov 2010
The University of Malawi, Chancellor College
BSc

Publications

17Publications

293Reads

619Profile Views

61PubMed Central Citations

Adaptation... that's what you need?

Nat Rev Microbiol 2017 Aug 10;15(8):452. Epub 2017 Jul 10.

Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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http://dx.doi.org/10.1038/nrmicro.2017.78DOI Listing
August 2017
8 Reads
23.574 Impact Factor

Population genetic structure, antibiotic resistance, capsule switching and evolution of invasive pneumococci before conjugate vaccination in Malawi.

Vaccine 2017 08 12;35(35 Pt B):4594-4602. Epub 2017 Jul 12.

Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S0264410X173090
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http://dx.doi.org/10.1016/j.vaccine.2017.07.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571440PMC
August 2017
53 Reads
3 Citations
3.624 Impact Factor

Genomic Epidemiology of Penicillin-Nonsusceptible Pneumococci with Nonvaccine Serotypes Causing Invasive Disease in the United States.

J Clin Microbiol 2017 04 18;55(4):1104-1115. Epub 2017 Jan 18.

Department of Epidemiology, Center for Communicable Disease Dynamics, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA

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http://jcm.asm.org/lookup/doi/10.1128/JCM.02453-16
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http://dx.doi.org/10.1128/JCM.02453-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377837PMC
April 2017
17 Reads
2 Citations
3.993 Impact Factor

The global distribution and diversity of protein vaccine candidate antigens in the highly virulent Streptococcus pnuemoniae serotype 1.

Vaccine 2017 02 9;35(6):972-980. Epub 2017 Jan 9.

Malawi-Liverpool Wellcome Trust Clinical Research Programme, Queen Elizabeth Central Hospital, Blantyre, Malawi; Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool L69 7BE, UK.

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http://dx.doi.org/10.1016/j.vaccine.2016.12.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287219PMC
February 2017
25 Reads
3 Citations
3.624 Impact Factor

Recombination in Streptococcus pneumoniae Lineages Increase with Carriage Duration and Size of the Polysaccharide Capsule

mBio

Streptococcus pneumoniae causes a high burden of invasive pneumococcal disease (IPD) globally, especially in children from resource-poor settings. Like many bacteria, the pneumococcus can import DNA from other strains or even species by transformation and homologous recombination, which has allowed the pneumococcus to evade clinical interventions such as antibiotics and pneumococcal conjugate vaccines (PCVs). Pneumococci are enclosed in a complex polysaccharide capsule that determines the serotype; the capsule varies in size and is associated with properties including carriage prevalence and virulence. We determined and quantified the association between capsule and recombination events using genomic data from a diverse collection of serotypes sampled in Malawi. We determined both the amount of variation introduced by recombination relative to mutation (the relative rate) and how many individual recombination events occur per isolate (the frequency). Using univariate analyses, we found an association between both recombination measures and multiple factors associated with the capsule, including duration and prevalence of carriage. Because many capsular factors are correlated, we used multivariate analysis to correct for collinearity. Capsule size and carriage duration remained positively associated with recombination, although with a reduced P value, and this effect may be mediated through some unassayed additional property associated with larger capsules. This work describes an important impact of serotype on recombination that has been previously overlooked. While the details of how this effect is achieved remain to be determined, it may have important consequences for the serotype-specific response to vaccines and other interventions.

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September 2016
15 Reads

Region-specific diversification of the highly virulent serotype 1 .

Microb Genom 2015 Aug 11;1(2):e000027. Epub 2015 Aug 11.

The Malawi-Liverpool-Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine, Blantyre, Malawi; University of Liverpool, Institute of Infection and Global Health, Liverpool, UK.

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http://mgen.microbiologyresearch.org/content/journal/mgen/10
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http://dx.doi.org/10.1099/mgen.0.000027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320570PMC
August 2015
19 Reads
3 Citations

High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children.

BMC Infect Dis 2015 Jun 20;15:234. Epub 2015 Jun 20.

Microbes, Immunity and Vaccines, Malawi Liverpool Wellcome Trust Clinical Research Programme, Blantyre, Malawi.

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http://dx.doi.org/10.1186/s12879-015-0980-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474563PMC
June 2015
12 Reads
17 Citations
2.613 Impact Factor

Region-specific diversification of the highly virulent serotype 1 Streptococcus pneumoniae

M Gen 1(2): doi:10.1099/mgen.0.000027

Microbial Genomics

Serotype 1 Streptococcus pneumoniae is a leading cause of invasive pneumococcal disease (IPD) worldwide, with the highest burden in developing countries. We report the whole-genome sequencing analysis of 448 serotype 1 isolates from 27 countries worldwide (including 11 in Africa). The global serotype 1 population shows a strong phylogeographic structure at the continental level, and within Africa there is further region-specific structure. Our results demonstrate that region-specific diversification within Africa has been driven by limited cross-region transfer events, genetic recombination and antimicrobial selective pressure. Clonal replacement of the dominant serotype 1 clones circulating within regions is uncommon; however, here we report on the accessory gene content that has contributed to a rare clonal replacement event of ST3081 with ST618 as the dominant cause of IPD in the Gambia.

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June 2015
7 Reads

Mechanisms and impact of genetic recombination in the evolution of Streptococcus pneumoniae.

Comput Struct Biotechnol J 2015 8;13:241-7. Epub 2015 Apr 8.

Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, L69 7BE Liverpool, UK.

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http://dx.doi.org/10.1016/j.csbj.2015.03.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404416PMC
April 2015
6 Reads
10 Citations