Publications by authors named "Chris Lodge"

3 Publications

  • Page 1 of 1

Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension.

EMBO J 2021 Apr 1;40(7):e106812. Epub 2021 Mar 1.

Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Genome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeric cohesin by shugoshin-PP2A seems evolutionarily conserved. However, deprotection has been proposed to rely on spindle forces separating the Rec8 protector from cohesin at metaphase II in mammalian oocytes and on APC/C-dependent destruction of the protector at anaphase II in yeast. Here, we have activated APC/C in the absence of sister kinetochore biorientation at meiosis II in yeast and mouse oocytes, and find that bipolar spindle forces are dispensable for sister centromere separation in both systems. Furthermore, we show that at least in yeast, protection of Rec8 by shugoshin and inhibition of separase by securin are both required for the stability of centromeric cohesin at metaphase II. Our data imply that related mechanisms preserve the integrity of dyad chromosomes during the short metaphase II of yeast and the prolonged metaphase II arrest of mammalian oocytes.
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http://dx.doi.org/10.15252/embj.2020106812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013787PMC
April 2021

Oocyte aneuploidy-more tools to tackle an old problem.

Proc Natl Acad Sci U S A 2020 06 19;117(22):11850-11852. Epub 2020 May 19.

Newcastle Fertility Centre, Biosciences Institute, Newcastle University, Newcastle upon Tyne NE1 4EP, United Kingdom

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http://dx.doi.org/10.1073/pnas.2005739117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275742PMC
June 2020

Asbestos--why is this subject being dragged up again?

Health Estate 2003 Jan;57(1):55-6

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January 2003