Publications by authors named "Chih Ling Wang"

5 Publications

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Stem Cell Metabolism and Diet.

Curr Stem Cell Rep 2020 Dec 28;6(4):119-125. Epub 2020 Oct 28.

Program in Metabolic Biology, Nutritional Sciences & Toxicology, University of California, Berkeley, CA 94720, 119 Morgan Hall, University of California, Berkeley, CA 94720.

Purpose Of Review: Diet has profound impacts on health and longevity. Evidence is emerging to suggest that diet impinges upon the metabolic pathways in tissue-specific stem cells to influence health and disease. Here, we review the similarities and differences in the metabolism of stem cells from several tissues, and highlight the mitochondrial metabolic checkpoint in stem cell maintenance and aging. We discuss how diet engages the nutrient sensing metabolic pathways and impacts stem cell maintenance. Finally, we explore the therapeutic implications of dietary and metabolic regulation of stem cells.

Recent Findings: Stem Cell transition from quiescence to proliferation is associated with a metabolic switch from glycolysis to mitochondrial OXPHOS and the mitochondrial metabolic checkpoint is critically controlled by the nutrient sensors SIRT2, SIRT3, and SIRT7 in hematopoietic stem cells. Intestine stem cell homeostasis during aging and in response to diet is critically dependent on fatty acid metabolism and ketone bodies and is influenced by the niche mediated by the nutrient sensor mTOR.

Summary: Nutrient sensing metabolic pathways critically regulate stem cell maintenance during aging and in response to diet. Elucidating the molecular mechanisms underlying dietary and metabolic regulation of stem cells provides novel insights for stem cell biology and may be targeted therapeutically to reverse stem cell aging and tissue degeneration.
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http://dx.doi.org/10.1007/s40778-020-00180-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992378PMC
December 2020

Pyruvate Responsiveness Based on α-Oxohydrazone Formation for Intracellular siRNA Release from Polyion Complex-Based Carriers.

Biomacromolecules 2019 06 23;20(6):2305-2314. Epub 2019 May 23.

Laboratory for Chemistry and Life Science, Institute of Innovative Research , Tokyo Institute of Technology , R1-11, 4259, Nagatsuta , Midori-Ku, Yokohama , Kanagawa 226-8503 , Japan.

Selective release of small interfering RNA (siRNA) payloads in response to intracellular substances is a prerequisite for the smart design of siRNA carriers. In this context, we developed a molecular program that allows reactivity with pyruvate for siRNA release in the cell on the basis of polyionic-complex- (PIC-) based siRNA carriers. Hydrazide can react with the α-keto acid structure of anionic pyruvate to form α-oxohydrazone, resulting in the reduction of the cationic net charge of the cationic polymer bearing a hydrazide moiety, which in turn leads to an inefficient electrostatic interaction with anionic siRNA and the consequent destabilization of the PIC (i.e., PGlu [DET/hydrazide]) in pyruvate-enriched environments, such as the cytoplasm, thus achieving effective siRNA release from the PIC and its associated gene-silencing activity. The present study provides the rationale for an α-oxohydrazone-formation-based smart design of pyruvate-responsive materials in the cell.
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http://dx.doi.org/10.1021/acs.biomac.9b00261DOI Listing
June 2019

An Unprecedented Interpenetrating Structure Built from Two Differently Bonded Frameworks: Synthesis, Characteristics, and Efficient Removal of Anionic Dyes from Aqueous Solutions.

Chemistry 2019 Jun 2;25(33):7815-7819. Epub 2019 May 2.

Institute of Bioscience and Biotechnology, National (Taiwan) Ocean University, Keelung, 202, Taiwan.

The first example of one single crystal (NTOU-5) containing two different organic-inorganic hybrid open-framework structures was obtained using a hydro(solvo)thermal method and structurally characterized by single-crystal X-ray diffraction. Remarkably, under the same synthetic conditions, the zinc ions are respectively coordinated by oxalic acid (OX) and 1,2,4,5-tetrakis(imidazol-1-ylmethyl)benzene (TIMB) linkers to form two significantly different frameworks: anionic [Zn (OX) ] and cationic [Zn(TIMB)] networks that interweave with each other to give an unprecedented interpenetrating structure with two differently-bonded open-frameworks. From the inorganic chemistry perspective, it is extremely difficult to control to which metal center the oxygen-donor linkers or/and nitrogen-donor ligands bind. A mixed Co/Zn analogue was also obtained by a similar method. The single-crystal XRD and EDS analyses indicate that the octahedral Zn ions of the anionic framework are replaced by cobalt cations, whereas the Zn ions in the tetrahedral positions of the cationic networks remain intact. This leads to the formation of the interpenetrating analogue with a mixed metal composition. Furthermore, NTOU-5 shows structural stability and efficiently removes organic dyes from aqueous solutions at concentrations of 10 ppm.
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http://dx.doi.org/10.1002/chem.201900607DOI Listing
June 2019

Dietary wild bitter gourd displays selective androgen receptor modulator like activity and improves the muscle decline of orchidectomized mice.

Food Funct 2019 Jan;10(1):125-139

Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan.

Loss of skeletal muscle mass and strength is often associated with disability and poor quality of life. Selective Androgen Receptor Modulators (SARMs) are under development as potential treatment. This study aims at examining the potential of wild bitter gourd (BG) as a SARM and its effects on the muscle decline induced by orchiectomy. In the cell-based androgen receptor (AR) transactivation assay, the BGP extract showed weak agonistic and antagonistic activities, resembling those of some SARMs. Male C57BL/6J mice were sham-operated (Sham group) or castrated (Cast groups) and fed a modified AIN-93G high sucrose diet supplemented without (Cast group) or with 5% lyophilized BG powder (Cast + BGP) or with testosterone propionate (7 mg TP per kg diet, Cast + TP) for 23 weeks. In contrast to the Cast + TP group, the BGP supplementation did not affect the serum testosterone concentration, and prostate and seminal vesicle mass. Both TP and BGP supplementation increased the weight of androgen responsive muscles, bulbocavernosus (BC) and levator ani (LA) (p < 0.05). The grip strength and the performance on a rotarod of the Cast + BGP group were comparable to those of the Cast + TP group (p > 0.05). The number of succinate dehydrogenase (SDH)-positive fibers of the Cast + BGP group was not significantly different from that of the Sham and Cast + TP groups (p > 0.05). The BGP supplementation up-regulated the Pgc1α, Ucp2 or Ucp3 gene expressions in skeletal muscles of castrated mice (p < 0.05). BGP showed some characteristics of the SARM and might improve skeletal muscle function through the up-regulation of mitochondrial biogenic genes and oxidative capacity, and ameliorated the castration-induced decline of skeletal muscle function in mice.
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http://dx.doi.org/10.1039/c8fo01777hDOI Listing
January 2019

New 3 D Tubular Porous Structure of an Organic-Zincophosphite Framework with Interesting Gas Adsorption and Luminescence Properties.

Chemistry 2016 Nov 10;22(45):16099-16102. Epub 2016 Oct 10.

Institute of Chemistry, Academia Sinica, Nankang, Taipei, 115, Taiwan.

A new 3D tubular zinc phosphite, Zn (C H N ) (HPO ) ⋅H O (1), incorporating a tetradentate organic ligand was synthesized under hydro(solvo)thermal conditions and structurally characterized by single-crystal X-ray diffraction. Compound 1 is the first example of inorganic zincophosphite chains being interlinked through 1,2,4,5-tetrakis(imidazol-1-ylmethyl)benzene to form a tubular porous framework with unusual organic-inorganic hybrid channels. The thermal and chemical stabilities, high capacity for CO adsorption compared to that for N adsorption, and interesting optical properties of LED devices fabricated using this compound were also studied.
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http://dx.doi.org/10.1002/chem.201602918DOI Listing
November 2016
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