Publications by authors named "Chi Yao"

64 Publications

DNA nanocomplex containing cascade DNAzymes and promoter-like Zn-Mn-Ferrite for combined gene/chemo-dynamic therapy.

Angew Chem Int Ed Engl 2021 Dec 5. Epub 2021 Dec 5.

Tianjin University, Chemistry Department, Room 328, Building 54, 300350, Tianjin, CHINA.

Sequential control of exogenous chemical events inside cells is a promising way to regulate cell functions and fate. Herein we report a DNA nanocomplex containing cascade DNAzymes and promoter-like Zn-Mn-Ferrite (ZMF), achieving combined gene/chemo-dynamic therapy. The promoter-like ZMF decomposed in response to intratumoral glutathione to release a sufficient quantity of metal ions, thus promoting cascade DNA/RNA cleavage and free radical generation. Two kinds of DNAzymes were designed for sequential cascade enzymatic reaction, in which metal ions functioned as cofactors. The primary DNAzyme self-cleaved the DNA chain with Zn2+ as cofactor, and produced the secondary DNAzyme; the secondary DNAzyme afterwards cleaved the EGR-1 mRNA, and thus downregulated the expression of target EGR-1 protein, achieving DNAzyme-based gene therapy. Meanwhile, the released Zn2+, Mn2+ and Fe2+ induced Fenton/Fenton-like reactions, during which free radicals were catalytically generated and efficient chemo-dynamic therapy was achieved. In a breast cancer mouse model, the administration of DNA nanocomplex led to a significant therapeutic efficacy of tumor growth suppression.
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http://dx.doi.org/10.1002/anie.202113619DOI Listing
December 2021

Research Progress of Microtransfer Printing Technology for Flexible Electronic Integrated Manufacturing.

Micromachines (Basel) 2021 Nov 3;12(11). Epub 2021 Nov 3.

Key Laboratory of Vibration and Control of Aeronautical Power Equipment of Ministry of Education, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China.

In recent years, with the rapid development of the flexible electronics industry, there is an urgent need for a large-area, multilayer, and high-production integrated manufacturing technology for scalable and flexible electronic products. To solve this technical demand, researchers have proposed and developed microtransfer printing technology, which picks up and prints inks in various material forms from the donor substrate to the target substrate, successfully realizing the integrated manufacturing of flexible electronic products. This review retrospects the representative research progress of microtransfer printing technology for the production of flexible electronic products and emphasizes the summary of seal materials, the basic principles of various transfer technology and fracture mechanics models, and the influence of different factors on the transfer effect. In the end, the unique functions, technical features, and related printing examples of each technology are concluded and compared, and the prospects of further research work on microtransfer printing technology is finally presented.
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http://dx.doi.org/10.3390/mi12111358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619378PMC
November 2021

T Lymphocyte-Captured DNA Network for Localized Immunotherapy.

J Am Chem Soc 2021 Nov 15;143(46):19330-19340. Epub 2021 Nov 15.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Institute of Biomolecular and Biomedical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, People's Republic of China.

The efficient isolation of immune cells with high purity and low cell damage is important for immunotherapy and remains highly challenging. We herein report a cell capture DNA network containing polyvalent multimodules for the specific isolation and incubation of T lymphocytes (T-cells). Two ultralong DNA chains synthesized by an enzymatic amplification process were rationally designed to include functional multimodules as cell anchors and immune adjuvants. Mutually complementary sequences facilitated the formation of a DNA network and encapsulation of T-cells, as well as offering cutting sites of a restriction enzyme for the responsive release of T-cells and immune adjuvants. The purity of captured tumor-infiltrating T-cells reached 98%, and the viability of T-cells maintained ∼90%. The T-cells-containing DNA network was further administrated to a tumor lesion for localized immunotherapy. Our work provides a robust nanobiotechnology for efficient isolation of immune cells and other biological particles.
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http://dx.doi.org/10.1021/jacs.1c07036DOI Listing
November 2021

Rolling circle amplification (RCA)-based DNA hydrogel.

Nat Protoc 2021 Dec 29;16(12):5460-5483. Epub 2021 Oct 29.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China.

DNA hydrogels have unique properties, including sequence programmability, precise molecular recognition, stimuli-responsiveness, biocompatibility and biodegradability, that have enabled their use in diverse applications ranging from material science to biomedicine. Here, we describe a rolling circle amplification (RCA)-based synthesis of 3D DNA hydrogels with rationally programmed sequences and tunable physical, chemical and biological properties. RCA is a simple and highly efficient isothermal enzymatic amplification strategy to synthesize ultralong single-stranded DNA that benefits from mild reaction conditions, and stability and efficiency in complex biological environments. Other available methods for synthesis of DNA hydrogels include hybridization chain reactions, which need a large amount of hairpin strands to produce DNA chains, and PCR, which requires temperature cycling. In contrast, the RCA process is conducted at a constant temperature and requires a small amount of circular DNA template. In this protocol, the polymerase phi29 catalyzes the elongation and displacement of DNA chains to amplify DNA, which subsequently forms a 3D hydrogel network via various cross-linking strategies, including entanglement of DNA chains, multi-primed chain amplification, hybridization between DNA chains, and hybridization with functional moieties. We also describe how to use the protocol for isolation of bone marrow mesenchymal stem cells and cell delivery. The whole protocol takes ~2 d to complete, including hydrogel synthesis and applications in cell isolation and cell delivery.
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http://dx.doi.org/10.1038/s41596-021-00621-2DOI Listing
December 2021

Supramolecular Self-Assembled DNA Nanosystem for Synergistic Chemical and Gene Regulations on Cancer Cells.

Angew Chem Int Ed Engl 2021 11 2;60(48):25557-25566. Epub 2021 Nov 2.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Institute of Biomolecular and Biomedical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Incorporating multiple molecular interactions within a system to realize the metabolic reprogramming of cancer cells is prospected to be of great potential in cancer therapy. Herein, we report a supramolecular self-assembled DNA nanosystem, which reprogrammed the cellular antioxidant system via synergistic chemical and gene regulations. In the nanosystem, amphipathic telluroether was coordinated with Mn to self-assemble into micelle, on which a siNrf2 integrated DNA network was assembled. The great electron-donating capability of telluroether was revealed to greatly promote Mn -based Fenton-like reaction to generate subversive OH in cancer cells. In response to adenosine triphosphoric acid, the siNrf2 was specially released in cytoplasm for down-regulating expression of detoxification enzymes, which enhanced chemocatalysis-mediated oxidative stress in cancer cells, thus significantly suppressing tumor progression.
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http://dx.doi.org/10.1002/anie.202111900DOI Listing
November 2021

Multimodules integrated functional DNA nanomaterials for intelligent drug delivery.

Wiley Interdiscip Rev Nanomed Nanobiotechnol 2021 Aug 30:e1753. Epub 2021 Aug 30.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.

Deoxyribonucleic acid (DNA) has been an emerging building block to construct functional biomaterials. Due to their programmable sequences and rich responsiveness, DNA has attracted rising attention in the construction of intelligent nanomaterials with predicable nanostructure and adjustable functions, which has shown great potential in drug delivery. On the one hand, the DNA sequences with molecule recognition, responsiveness, and therapeutic efficacy can be easily integrated to the framework of DNA nanomaterials by sequence designing; on the other hand, the rich chemical groups on DNA molecules provide binding points for other functional units. In this review, we divided the functionalization modules in the construction of DNA nanomaterials into three types, including targeting modules, responsive modules, and therapeutic modules. Based on these modules, five DNA kinds of representative nanomaterials applied in drug delivery were introduced, including DNA nanogel, DNA origami, DNA framework, DNA nanoflower, and DNA hybrid nanosphere. Finally, we discussed the challenges in the transition of DNA materials to clinical applications. We expect that this review can help readers to obtain a deeper understanding of DNA materials, and further promote the development of these intelligent materials to real world's application. This article is categorized under: Biology-Inspired Nanomaterials > Nucleic Acid-Based Structures Nanotechnology Approaches to Biology > Nanoscale Systems in Biology.
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http://dx.doi.org/10.1002/wnan.1753DOI Listing
August 2021

Recent Progress of Extracellular Vesicle Engineering.

ACS Biomater Sci Eng 2021 09 28;7(9):4430-4438. Epub 2021 Aug 28.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P.R. China.

Extracellular vesicles (EVs) are nanoscale phospholipid bilayer membrane vesicles which contain varied active biomolecules. As natural carriers, EVs can deliver endogenous cargos to target tissues safely and effectively. However, the applications of natural released EVs are limited by their low yield and heterogeneity. Engineering EVs can endow them with more functions and better performances to address these issues. EVs can be modified and engineered to improve the yield, targeting efficiency, and content of beneficial cargos. Herein, the strategies of engineering EVs through genetic modification of EVs are introduced; the molecular modification of the EV membrane and the loading of nucleic acids are summarized; the building of EV mimetic nanovesicles are reviewed. Overall, we anticipate that readers will gain a better understanding of the progress of EV engineering, which will help to promote the development of the technologies and applications in this field.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00868DOI Listing
September 2021

Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition.

Cells 2021 07 5;10(7). Epub 2021 Jul 5.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in whole body was significantly reduced. BtVAMP2 was found to be conserved among the species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly.
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http://dx.doi.org/10.3390/cells10071700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306474PMC
July 2021

A review on organophosphate flame retardants in the environment: Occurrence, accumulation, metabolism and toxicity.

Sci Total Environ 2021 Nov 3;795:148837. Epub 2021 Jul 3.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, HoHai University, Nanjing 210098, China. Electronic address:

Organophosphate flame retardants (OPFRs), as a substitute for brominated flame retardants (BFRs), are widely used in industrial production and life. The presence of OPFRs in the environment has an adverse effect on the ecological environment system. This review provides comprehensive data for the occurrence of OPFRs and their diester metabolites (OP diesters) in wastewater treatment plants, surface water, drinking water, sediment, soil, air and dust in the environment. In particular, the accumulation and metabolism of OPFRs in organisms and the types of metabolites and metabolic pathways are discussed for animals and plants. In addition, the toxicity of OP triesters and OP diesters in organisms is discussed. Although research on OPFRs has gradually increased in recent years, there are still many gaps to be filled, especially for metabolic and toxicity mechanisms that need in-depth study. This review also highlights the shortcomings of current research and provides suggestions for a basis for future research on OPFRs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148837DOI Listing
November 2021

Polycyclic Aromatic Hydrocarbons and the Risk of Kidney Stones in US Adults: An Exposure-Response Analysis of NHANES 2007-2012.

Int J Gen Med 2021 21;14:2665-2676. Epub 2021 Jun 21.

Department of Urology, Zhongda Hospital, Southeast University, Nanjing, 210009, People's Republic of China.

Background: Polycyclic aromatic hydrocarbons (PAHs) exposure may cause various diseases. However, the association between PAHs exposure and kidney stones remains unclear. The purpose of this study was to examine the relationship between PAHs and the risk of kidney stones in the US population.

Methods: The study included a total of 30,442 individuals (≥20 years) from the 2007-2012 National Health and Nutrition Examination Survey (NHANES). Nine urinary PAHs were included in this study. Logistic regression and dose-response curves were used to evaluate the association between PAHs and the risk of kidney stones.

Results: We selected 4385 participants. The dose-response curves showed a significant positive association between total PAHs, 2-hydroxynaphthalene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 9-hydroxyfluorene and the risk of kidney stones after adjusting for confounding factors. Compared with the low group, an increased risk of kidney stones was observed in the high group of total PAHs [OR (95% CI), 1.32 (1.06-1.64), P=0.013], 2-hydroxynaphthalene [OR (95% CI), 1.37 (1.10-1.71), P=0.005], 1-hydroxyphenanthrene [OR (95% CI), 1.24 (1.00-1.54), P=0.046] and 9-hydroxyfluorene [OR (95% CI), 1.36 (1.09-1.70), P=0.007].

Conclusion: High levels of PAHs were positively associated with the risk of kidney stones in the US population.
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http://dx.doi.org/10.2147/IJGM.S319779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232959PMC
June 2021

A dye combination for the staining of pollen coat and pollen wall.

Plant Reprod 2021 06 26;34(2):91-101. Epub 2021 Apr 26.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

The pollen coat, which forms on the pollen surface, consists of a lipid-protein matrix. It protects pollen from desiccation and is involved in adhesion, pollen-stigma recognition, and pollen hydration during interactions with the stigma. The classical methods used for pollen coat observation are scanning and transmission electron microscopy. In this work, we screened a collection of fluorescence dyes and identified two fluorescent brighteners FB-52 and FB-184. When they were used together with the exine-specific dye, Basic fuchsin, the pollen coat and the exine structures could be clearly visualized in the pollen of Brassica napus. This co-staining method was applied successfully in staining pollen from Fraxinus chinensis, Calystegia hederacea, and Petunia hybrida. Using this method, small pollen coat-containing cavities were detected in the outer pollen wall layer of Oryza sativa and Zea mays. We further showed these dyes are compatible with fluorescent protein markers. In the Arabidopsis thaliana transgenic line of GFP-tagged pollen coat protein GRP19, GRP19-GFP was observed to form particles at the periphery of pollen coat. This simple staining method is expected to be widely used for the studies of the palynology as well as the pollen-stigma interaction.
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http://dx.doi.org/10.1007/s00497-021-00412-5DOI Listing
June 2021

Development of the Middle Layer in the Anther of .

Front Plant Sci 2021 10;12:634114. Epub 2021 Feb 10.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.

The middle layer is an essential cell layer of the anther wall located between the endothecium and tapetum in . Based on sectioning, the middle layer was found to be degraded at stage 7, which led to the separation of the tapetum from the anther wall. Here, we established techniques for live imaging of the anther. We created a marker line with fluorescent proteins expressed in all anther layers to study anther development. Several staining methods were used in the intact anthers to study anther cell morphology. We clarified the initiation, development, and degradation of the middle layer in . This layer is initiated from both the inner and outer secondary parietal cells at stage 4, stopped cell division at stage 6, and finally degraded at stage 11. The neighboring cell layers, the epidermis, and endothecium continued cell division until stage 10, which led to a thin middle layer. The degradation of the tapetum cell wall at stage 7 lead to its isolation from the anther wall. This work presents fundamental information on the development of the middle layer, which facilitates the further investigation of anther development and plant fertility. These live imaging methods could be useful in future studies.
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http://dx.doi.org/10.3389/fpls.2021.634114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902515PMC
February 2021

Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus.

Microorganisms 2021 Feb 2;9(2). Epub 2021 Feb 2.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.
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http://dx.doi.org/10.3390/microorganisms9020304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912986PMC
February 2021

Dual Roles of Metal-Organic Frameworks as Nanocarriers for miRNA Delivery and Adjuvants for Chemodynamic Therapy.

ACS Appl Mater Interfaces 2021 Feb 26;13(5):6034-6042. Epub 2021 Jan 26.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China.

MicroRNA (miRNA) represents a promising class of therapeutic nucleic acid drugs, while delivery challenges remain that impede the advancement of miRNA therapy, largely because of in vivo instability and low delivery efficiency. Herein, we discover the dual roles of metal-organic framework (MOF) nanoparticles (ZIF-8) as nanocarriers for miRNA delivery and adjuvants for chemodynamic therapy. The [email protected] complex demonstrated efficient cellular uptake and lysosomal stimuli-responsive miRNA release. Zn triggered the generation of reactive oxygen species, which consequently induced apoptosis of tumor cells. Released miR-34a-m led to a remarkable decrease in expression of Bcl-2 at both mRNA and protein levels and enhanced cancer cell apoptosis. In vivo experiments showed high efficacy of using [email protected] to suppress tumor growth via synergistic gene/chemodynamic therapy in a mouse model of triple-negative breast cancer. Our work demonstrates MOFs as a promising nanoplatform for efficient synergetic gene/chemodynamic therapy.
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http://dx.doi.org/10.1021/acsami.0c21006DOI Listing
February 2021

Clinical efficacy of platelet-rich plasma as adjuvant therapy in patients undergoing arthroscopic repair of meniscal injury.

J Int Med Res 2020 Sep;48(9):300060520955059

Department of Hand Surgery, the Second Hospital of Jilin University, Changchun, Jilin Province, China.

Objective: The clinical efficacy of platelet-rich plasma (PRP) as adjuvant therapy in patients undergoing arthroscopic repair of meniscal injury remains controversial. This meta-analysis was performed to evaluate the clinical efficacy of PRP in the treatment of meniscal injury and provide evidence for the selection of clinical treatment options.

Methods: A computer-based search of the PubMed, Embase, and Cochrane Library databases was performed to retrieve articles using the search terms "platelet-rich plasma" and "menisci." Quality evaluation and data extraction were performed. The combined effect was assessed using RevMan version 5.3 software.

Results: Three randomized controlled trials and three cohort studies involving 293 patients were included in the meta-analysis. There were no significant differences in the International Knee Documentation Committee score or Lysholm score between the experimental and control groups. The failure rate and visual analog scale score were significantly lower and the degree of active flexion was significantly higher in the experimental group than in the control group.

Conclusion: The findings of this meta-analysis suggest that PRP injection can effectively enhance the efficacy of arthroscopic repair of meniscal injury, reduce the failure rate and severity of pain, and improve active flexion.
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http://dx.doi.org/10.1177/0300060520955059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520921PMC
September 2020

Self-assembly of stem cell membrane-camouflaged nanocomplex for microRNA-mediated repair of myocardial infarction injury.

Biomaterials 2020 10 25;257:120256. Epub 2020 Jul 25.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, PR China. Electronic address:

Mesenchymal stem cells-derived exosomes have shown promising therapeutic effect on myocardial infarction (MI). The major hurdles remain for the use of exosomes primarily due to the low yields from cell cultures coupled with complicated purification processes. Herein we report the self-assembly of stem cell membrane-camouflaged exosome-mimicking nanocomplex that recapitulates exosome functions, achieving efficient microRNA (miRNA) delivery and miRNA-mediated myocardial repair. The nanocomplex is constructed via the self-assembly of mesenchymal stem cell membrane on miRNA loaded mesoporous silica nanoparticle surface, which enables high miRNA loading capacity and protects miRNA from degradation in body fluid. The nanocomplex can escape the clearance of immunologic system, and target to ischemic injured cardiomyocytes. miRNA is triggered to release and binds to target mRNA, which inhibits the translation of apoptosis-related proteins, and consequently promotes the proliferation of cardiomyocytes. In the MI mouse model, the administration of exosome-mimicking nanocomplex effectively leads to preservation of viable myocardium and augmentation of cardiac functions.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120256DOI Listing
October 2020

DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications.

Chem Rev 2020 09 16;120(17):9420-9481. Epub 2020 Jul 16.

Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China.

DNA is traditionally known as a central genetic biomolecule in living systems. From an alternative perspective, DNA is a versatile molecular building-block for the construction of functional materials, in particular biomaterials, due to its intrinsic biological attributes, molecular recognition capability, sequence programmability, and biocompatibility. The topologies of DNA building-blocks mainly include linear, circular, and branched types. Branched DNA recently has been extensively employed as a versatile building-block to synthesize new biomaterials, and an assortment of promising applications have been explored. In this review, we discuss the progress on DNA functional materials assembled from branched DNA. We first briefly introduce the background information on DNA molecules and sketch the development history of DNA functional materials constructed from branched DNA. In the second part, the synthetic strategies of branched DNA as building-blocks are categorized into base-pairing assembly and chemical bonding. In the third part, construction strategies for the branched DNA-based functional materials are comprehensively summarized including tile-mediated assembly, DNA origami, dynamic assembly, and hybrid assembly. In the fourth part, applications including diagnostics, protein engineering, drug and gene delivery, therapeutics, and cell engineering are demonstrated. In the end, an insight into the challenges and future perspectives is provided. We envision that branched DNA functional materials can not only enrich the DNA nanotechnology by ingenious design and synthesis but also promote the development of interdisciplinary fields in chemistry, biology, medicine, and engineering, ultimately addressing the growing demands on biological and medical-related applications in the real world.
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http://dx.doi.org/10.1021/acs.chemrev.0c00294DOI Listing
September 2020

Small Animal Shanoir (SAS) A Cloud-Based Solution for Managing Preclinical MR Brain Imaging Studies.

Front Neuroinform 2020 19;14:20. Epub 2020 May 19.

INSERM U1216, Grenoble Institut des Neurosciences, Université Grenoble Alpes, CHU Grenoble Alpes, Grenoble, France.

Clinical multicenter imaging studies are frequent and rely on a wide range of existing tools for sharing data and processing pipelines. This is not the case for preclinical (small animal) studies. Animal population imaging is still in infancy, especially because a complete standardization and control of initial conditions in animal models across labs is still difficult and few studies aim at standardization of acquisition and post-processing techniques. Clearly, there is a need of appropriate tools for the management and sharing of data, post-processing and analysis methods dedicated to small animal imaging. Solutions developed for Human imaging studies cannot be directly applied to this specific domain. In this paper, we present the Small Animal Shanoir (SAS) solution for supporting animal population imaging using tools compatible with open data. The integration of automated workflow tools ensures accessibility and reproducibility of research outputs. By sharing data and imaging processing tools, hosted by SAS, we promote data preparation and tools for reproducibility and reuse, and participation in multicenter or replication "" studies contributing to the improvement of quality science in preclinical domain. SAS is a first step for promoting open science for small animal imaging and a contribution to the valorization of data and pipelines of reference.
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http://dx.doi.org/10.3389/fninf.2020.00020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248267PMC
May 2020

Ni/Co bimetallic organic framework nanosheet assemblies for high-performance electrochemical energy storage.

Nanoscale 2020 May;12(19):10685-10692

AIST-Kyoto University Chemical Energy Materials Open Innovation Laboratory (ChEM-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.

Nickel-cobalt organic framework (denoted as NiCo-MOF) nanosheet assemblies are prepared through a controllable one-pot hydrothermal synthesis procedure at 150 °C. The as-prepared samples are directly employed as electrode materials for electrochemical energy storage (EES), and exhibit excellent electrochemical performance. Among these samples, NiCo-MOF-1 displays a high capacity of 100.18 mA h g-1 (901.60 F g-1), and obtains a capacity retention of 81.00% over 3000 cycles at 5 A g-1. Likewise, in an aqueous device, NiCo-MOF-1//AC delivers a discharge capacity of 83.75 mA h g-1, and also exhibits a good cycling life (74.14% retention after 3000 cycles). These results demonstrate that multilayer NiCo-MOF nanosheet assemblies are potential electrode materials for EES.
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http://dx.doi.org/10.1039/d0nr02016hDOI Listing
May 2020

Multiresponsive White-Light Emitting Aerogel Prepared with Codoped Lanthanide/Thymidine/Carbon Dots.

ACS Appl Mater Interfaces 2020 May 30;12(19):22191-22199. Epub 2020 Apr 30.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, People's Republic of China.

Aerogels hold great promise as a lightweight replacement in materials fields. Dynamic fluorochromic aerogels that possess reversible stimuli responsiveness have been particularly attractive recently for new design opportunities in practical solid-state lighting and wide applications in advanced sensors/probe. In this study, we report a reversibly multiresponsive white-light-emitting (WLE) aerogel prepared with codoped lanthanide, thymidine, and carbon dots. By precisely modulating the stoichiometric ratio of lanthanide complexes and carbon dots, broad-spectrum output from purple to red is obtained, including pure white light (CIE (0.33, 0.32)). The freeze-drying process contributes to the elimination of hydration between water molecules and lanthanide ions, further preventing the quenching of lanthanide luminescence and preserving the high quantum yield (47.4%) of our aerogel. Moreover, the dynamic coordination bond between lanthanide (europium and terbium) and thymidine endows the aerogel with reversible responsiveness upon five different stimuli, including halide anions, metal ions, pH, temperature, and humidity. We envision that our WLE aerogel has considerable potential for use in various fields such as display devices, advanced sensors, and environmentally friendly probes where multiresponsiveness is required.
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http://dx.doi.org/10.1021/acsami.0c04253DOI Listing
May 2020

Chiral Carbon Dots Mimicking Topoisomerase I To Mediate the Topological Rearrangement of Supercoiled DNA Enantioselectively.

Angew Chem Int Ed Engl 2020 06 28;59(27):11087-11092. Epub 2020 Apr 28.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Nanomaterials with enzyme-mimetic activities are possible alternatives to natural enzymes. Mimicking enzymatic enantioselectivity remains a great challenge. Herein, we report that cysteine-derived chiral carbon dots (CDs) can mimic topoisomerase I to mediate topological rearrangement of supercoiled DNA enantioselectively. d-CDs can more effectively catalyze the topological transition of plasmid DNA from supercoiled to nicked open-circular configuration than l-CDs. Experiments suggest the underlying mechanism: d-CDs intercalatively bind with DNA double helix more strongly than l-CDs; the intercalative CDs can catalyze the production of hydroxyl radicals to cleave phosphate backbone in one strand of the double helix, leading to topological rearrangement of supercoiled DNA. Molecular dynamics (MD) simulation show that the stronger affinity for hydrogen-bond formation and hydrophobic interaction between d-cysteine and DNA than that of l-cysteine is the origin of enantioselectivity.
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http://dx.doi.org/10.1002/anie.202002904DOI Listing
June 2020

Mutations in the coat protein of a begomovirus result in altered transmission by different species of whitefly vectors.

Virus Evol 2020 Jan 4;6(1):veaa014. Epub 2020 Mar 4.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

For many crop pathogens including viruses, high genetic variation provides them with potential to adapt to and prevail in a changing environment. Understanding genetic variation in viruses and their significance is a key to elaborate virus epidemiology and evolution. While genetic variation of plant viruses has been documented to impact virus-host interactions, how it affects virus-insect vector interactions remains elusive. Here, we report the impact of mutations in the coat protein of squash leaf curl China virus (SLCCNV), a begomovirus, on the interaction between the virus and its whitefly vectors. We characterized mutations in the coat protein of SLCCNV and found that some residues exhibited higher mutation frequency than the others. We assayed the impact of mutation on infectivity using agroinoculation and found these mutations marginally affect virus infectivity. We further analyze their functions using virus acquisition and transmission trials and found some of mutations resulted in altered transmission of SLCCNV by different species of the whitefly complex. We then identified the key amino acid residue(s) involved by constructing several mutant viruses and found that a single-residue mutation in the coat protein of SLCCNV was sufficient to significantly alter the whitefly transmission characteristics of SLCCNV. We examined the competition between different genotypes of SLCCNV in plant infection and whitefly transmission. We found that mutations in the coat protein did not alter the fitness of SLCCNV in plants, but they rendered the virus more competitive in transmission by certain species of whiteflies. Our findings indicate that mutations in the coat protein may play a key role in both the adaptation of begomoviruses to the changing vector populations and the evolution of begomoviruses.
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http://dx.doi.org/10.1093/ve/veaa014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055206PMC
January 2020

Double Rolling Circle Amplification Generates Physically Cross-Linked DNA Network for Stem Cell Fishing.

J Am Chem Soc 2020 02 9;142(7):3422-3429. Epub 2020 Jan 9.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , People's Republic of China.

Stem cells have been widely studied in cell biology and utilized in cell-based therapies, and fishing stem cells from marrow is highly challenging due to the ultralow content. Herein, a physically cross-linked DNA network-based cell fishing strategy is reported, achieving efficient capture, 3D envelop, and enzyme-triggered release of bone marrow mesenchymal stem cells (BMSCs). DNA network is constructed via a double rolling circle amplification method and through the intertwining and self-assembly of two strands of ultralong DNA chains. DNA-chain-1 containing aptamer sequences ensures specific anchor with BMSCs from marrow. Hybridization between DNA-chain-1 and DNA-chain-2 enables the cross-link of cell-anchored DNA chains to form a 3D network, thus realizing cell envelop and separation. DNA network creates a favorable microenvironment for 3D cell culture, and remarkably the physically cross-linked DNA network shows no damage to cells. DNA network is digested by nuclease, realizing the deconstruction from DNA network to fragments, and achieving enzyme-triggered cell release; after release, the activity of cells is well maintained. The strategy provides a powerful and effective method for fishing stem cells from tens of thousands of nontarget cells.
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http://dx.doi.org/10.1021/jacs.9b11001DOI Listing
February 2020

Occurrence and ecological implications of organophosphate triesters and diester degradation products in wastewater, river water, and tap water.

Environ Pollut 2020 Apr 18;259:113810. Epub 2019 Dec 18.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, HoHai University, Nanjing, 210098, China. Electronic address:

The occurrence and composition profiles of 13 triester organophosphate flame retardants and their three diester metabolites in river water, wastewater, and tap water in China were studied. Most target organophosphate esters (OPEs) were found in water samples, with average concentrations of 787 ng/L for triethyl phosphate (TEP) and 0.1 ng/L for tripropyl phosphate (TPP) in wastewater, 1.48 × 10 ng/L for TEP and 0.12 ng/L for tripentyl phosphate (TPeP) in river water, and 15.5 ng/L for tris(2-chloroethyl) phosphate (TCEP) and 0.08 ng/L for tritolyl phosphate (TMPP) in tap water. TEP was the most abundant compound among the detected OPEs in all water types. The exposure of zebrafish embryos showed negligible effects of TEP, triphenyl phosphate (TPHP), and diphenyl phosphate (DPHP), while mixed solutions that mimic river water and wastewater composition disturbed the development of embryos and led to the altered transcription of genes relating to the hypothalamic-pituitary-thyroid (HPT) axis. In addition, the binding affinity between OPEs and a thyroid hormone receptor (TRβ) protein was further investigated by molecular docking modeling, which helped to estimate the effects of OPEs on TRβ. This research provides experimental and theoretical evidence for the ecotoxicological effects of OPEs in aquatic environments.
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http://dx.doi.org/10.1016/j.envpol.2019.113810DOI Listing
April 2020

Differential transmission of Sri Lankan cassava mosaic virus by three cryptic species of the whitefly Bemisia tabaci complex.

Virology 2020 01 24;540:141-149. Epub 2019 Nov 24.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address:

In recent years, Sri Lankan cassava mosaic virus (SLCMV), a begomovirus (genus Begmovirus, family Geminiviridae) causing cassava mosaic disease in Asia, poses serious threats to cassava cultivation in Asia. However, the transmission of SLCMV in the areas into which it has recently been introduced remain largely unexplored. Here we have compared the transmission efficiencies of SLCMV by three widely distributed whitefly species in Asia, and found that only Asia II 1 whiteflies were able to transmit this virus efficiently. The transmission efficiencies of SLCMV by different whitefly species were found to correlate positively with quantity of virus in whitefly whole body. Further, the viral transmission efficiency was found to be associated with varied ability of virus movement within different species of whiteflies. These findings provide detailed information regarding whitefly transmission of SLCMV, which will help to understand the spread of SLCMV in the field, and facilitate the prediction of virus epidemics.
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http://dx.doi.org/10.1016/j.virol.2019.11.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971692PMC
January 2020

Persistent Luminescent Nanoparticles Containing Hydrogels for Targeted, Sustained, and Autofluorescence-Free Tumor Metastasis Imaging.

Nano Lett 2020 01 9;20(1):252-260. Epub 2019 Dec 9.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , P. R. China.

Metastasis is the primary cause of cancer morbidity and mortality. To obtain an effective diagnosis and treatment, precise imaging of tumor metastasis is required. Here we prepared persistent luminescent nanoparticles (PLNPs) containing a hydrogel (PL-gel) for targeted, sustained, and autofluorescence-free tumor metastasis imaging. PLNPs offered renewable long-lasting near-infrared (NIR) emitting without in situ radiation, favoring deep tissue penetration imaging without background interference. PLNPs were conjugated with 4-carboxyphenyl boronic acid (CPBA) to yield PLNPs-CPBA, which specifically recognized metastatic breast cancer cells (MBA-MD-231 cells) and enabled receptor-mediated endocytosis for specific cancer cell labeling. The PLNPs-CPBA-labeled cancer cells enabled sensitive imaging performance and high viability without influencing the migration and invasiveness of cancer cells for long-term tracking. PLNPs-CPBA were further encapsulated inside alginate to generate PL-gel for sustained PLNPs-CPBA release and tumor cell labeling, and the PL-gel showed enhanced renewable persistent luminescence compared to the PLNPs-CPBA suspension. The metastasis in the mouse breast cancer model was continuously tracked by persistent luminescence imaging, showing that PL-gel achieved noninvasive and highly selective imaging of tumor metastasis without background interference. Our PL-gel could be rationally designed to specifically target other types of cancer cells and thus provide a powerful and generic platform for the study of tumor metastasis.
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http://dx.doi.org/10.1021/acs.nanolett.9b03755DOI Listing
January 2020

Distribution, Removal, and Risk Assessment of Pharmaceuticals and Their Metabolites in Five Sewage Plants.

Int J Environ Res Public Health 2019 11 27;16(23). Epub 2019 Nov 27.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.

The extensive use of pharmaceuticals and personal care products (PPCPs) leads to a continuous increase of their presence in urban wastewater. These pollutants are discharged into natural waters and pose a threat to human health and the ecological environment. This study focused on five sewage treatment plants in three cities of China's Yangtze River Delta as research sites to study the distribution and degradation of drugs and their conversion products in wastewater. The concentration of target compounds in the water ranged from 0 to 510.8 ng/L, and both positive and negative removal rates occurred during the treatment. Acetaminophen (ACE) and ibuprofen (IPF) can be completely removed in the biological treatment stage. The addition of flocculants and sand filtration has a positive effect on the removal of naproxen (NPX) and bezafibrate (BZB). Ultraviolet disinfection is beneficial for the removal of antipyrine (ATP) and diclofenac (DCF). A small amount of PPCPs were found in the sludge and particulate matter, which had little effect on removal. Finally, the risk quotients were used to evaluate the harmfulness of the PPCPs detected in the effluent to the ecological environment, and the results showed that there was little hazard.
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http://dx.doi.org/10.3390/ijerph16234729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926846PMC
November 2019

Super-Soft and Super-Elastic DNA Robot with Magnetically Driven Navigational Locomotion for Cell Delivery in Confined Space.

Angew Chem Int Ed Engl 2020 02 23;59(6):2490-2495. Epub 2019 Dec 23.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Soft organisms such as earthworms can access confined, narrow spaces, inspiring scientists to fabricate soft robots for in vivo manipulation of cells or tissues and minimally invasive surgery. We report a super-soft and super-elastic magnetic DNA hydrogel-based soft robot (DNA robot), which presents a shape-adaptive property and enables magnetically driven navigational locomotion in confined and unstructured space. The DNA hydrogel is designed with a combinational dynamic and permanent crosslinking network through chain entanglement and DNA hybridization, resulting in shear-thinning and cyclic strain properties. DNA robot completes a series of complex magnetically driven navigational locomotion such as passing through narrow channels and pipes, entering grooves and itinerating in a maze by adapting and recovering its shape. DNA robot successfully works as a vehicle to deliver cells in confined space by virtue of the 3D porous networked structure and great biocompatibility.
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http://dx.doi.org/10.1002/anie.201913549DOI Listing
February 2020

Multiresponsive Supramolecular Luminescent Hydrogels Based on a Nucleoside/Lanthanide Complex.

ACS Appl Mater Interfaces 2019 Dec 6;11(50):47404-47412. Epub 2019 Dec 6.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , P. R. China.

Supramolecular luminescent hydrogels based on natural molecules have shown high potential for a variety of applications because of unique optical properties and biocompatibility, particularly serving as advanced biomaterials for bioimaging, biosensing, cell engineering, and so forth. A lanthanide complex-based system provides a promising way to prepare supramolecular luminescent hydrogels. Herein, we realize the creation of a luminescent hydrogel assembled from lanthanides and nucleosides. Nucleosides, the essential components of nucleic acids, functioning as the ligands, successfully chelate with lanthanides and form complexes in water. The complexes subsequently serve as building-blocks to form supramolecular hydrogels, which exhibit characteristic luminescent emission of lanthanides. The coordination modes and forming mechanism are studied by electrospray ionization time-of-flight mass spectrometry, matrix-assisted laser desorption/ionization time of flight mass spectrometry, H NMR spectroscopy, and Fourier transform infrared spectroscopy; the corresponding molecular simulations are presented, and macro-/micro-morphologies, mechanical properties, and luminescent performances of hydrogels are systemically studied. Remarkably, these luminescent hydrogels show fluorochromic properties in response to external stimuli, including pH, temperature, anions, and cations, which are thus adopted to design smart luminescent switches and detect specific species such as Cu. Our work provides a feasible strategy to prepare stimuli-responsive luminescent hydrogels, reveals the diverse potential of nucleoside-based hydrogels, and exhibits a novel pathway for the preparation of smart optical materials.
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http://dx.doi.org/10.1021/acsami.9b17236DOI Listing
December 2019

Target-Triggered Polymerization of Branched DNA Enables Enzyme-free and Fast Discrimination of Single-Base Changes.

iScience 2019 Nov 18;21:228-240. Epub 2019 Oct 18.

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China. Electronic address:

Single-base changes lead to important biological and biomedical implications; however, the discrimination of single-base changes from normal DNA always remains a grand challenge. Herein we developed a DNA recognition and amplification system based on artificial branched DNA, namely, target-triggered polymerization (TTP), to realize enzyme-free and fast discrimination of single-base changes. Branched DNA as monomers rapidly polymerized into DNA nanospheres only with the trigger of specific DNA. Our TTP system worked reliably over a wide range of conditions. Remarkably, our TTP system was capable of discriminating base-changing DNA from normal DNA, including distinguishing 1-4 nucleotide changes and positions of single base, which was attributed to the significant amplification of small differences in hybridization thermodynamics and kinetics. We further proposed a theoretical method for calculating the hybridization probability of nucleic acids, which performed highly consistent with experimental results.
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http://dx.doi.org/10.1016/j.isci.2019.10.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838547PMC
November 2019
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