Publications by authors named "Chenyang Xu"

99 Publications

High-throughput sequencing identified circular RNA circUBE2K mediating RhoA associated bladder cancer phenotype via regulation of miR-516b-5p/ARHGAP5 axis.

Cell Death Dis 2021 Jul 20;12(8):719. Epub 2021 Jul 20.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Bladder cancer (BC) is known as a common and lethal urinary malignancy worldwide. Circular RNAs (circRNAs), an emerging non-coding RNA, participate in carcinogenesis process of several cancers including BC. In this study, high-throughput sequencing and RT-qPCR were applied to discover and validate abnormal high expression of circUBE2K in BC tissues. Fluorescence in situ hybridization (FISH) was used to detect hsa_circ_0009154 (circUBE2K) expression and subcellular localization in BC tissues. High circUBE2K predicted unfavorable prognoses in BCs, as well as correlated with clinical features. CCK8, transwell, EdU and wound healing assays demonstrated down-regulating circUBE2K decreased BC cell phenotype as proliferation, invasion, and migration, respectively. Further studies showed that circUBE2K promoted BC progression via sponging miR-516b-5p and enhancing ARHGAP5 expression through regulating RhoA activity. Dual-luciferase reporter, FISH and RNA pulldown assays were employed to verify the relationships among circUBE2K/miR-516b-5p/ARHGAP5/RhoA axis. Down-regulating miR-516b-5p or overexpressing ARHGAP5 restored RhoA activity mediated BC cell properties after silencing circUBE2K. Subcutaneous xenograft and metastasis model identified circUBE2K significantly increased BC cell metastasis and proliferation in-vivo. Taken together, we found that circUBE2K is a tumor-promoting circRNA in BC that functions as a ceRNA to regulate ARHGAP5 expression via sponging miR-516b-5p.
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http://dx.doi.org/10.1038/s41419-021-03977-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292476PMC
July 2021

Comparing ameliorative effects of biomass ash and alkaline slag on an acidic Ultisol under artificial Masson pine: A field experiment.

J Environ Manage 2021 Jul 16;297:113306. Epub 2021 Jul 16.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, P.O. Box 821, Nanjing, 210008, PR China.

Forest soil acidification caused by acid deposition is a serious threat to the forest ecosystem. To investigate the liming effects of biomass ash (BA) and alkaline slag (AS) on the acidic topsoil and subsoil, a three-year field experiment under artificial Masson pine was conducted at Langxi, Anhui province in Southern China. The surface application of BA and AS significantly increased the soil pH, and thus decreased exchangeable acidity and active Al in the topsoil. Soil exchangeable Ca and Mg in topsoil were significantly increased by the surface application of BA and AS, while an increase in soil exchangeable K was only observed in BA treatments. The soil acidity and active Al in subsoil were decreased by the surface application of AS. Compared with the control, soluble monomeric and exchangeable Al in the subsoil was decreased by 38.0% and 29.4% after 3 years of AS surface application. There was a minimal effect on soluble monomeric and exchangeable Al after the application of BA. The soil exchangeable Ca and Mg in the subsoil increased respectively by 54% and 141% after surface application of 10 t ha AS. The decrease of soil active Al and increase of base cations in subsoil were mainly attributed to the high migration capacity of base cations in AS. In conclusion, the effect of surface application of AS was superior to BA in ameliorating soil acidity and alleviating soil Al toxicity in the subsoil of this Ultisol.
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http://dx.doi.org/10.1016/j.jenvman.2021.113306DOI Listing
July 2021

A novel glucose-based highly selective phosphate adsorbent.

Sci Total Environ 2021 Jun 16;792:148452. Epub 2021 Jun 16.

Agricultural Technology Extension Station, Mian County, Hanzhong 724200, China.

Industrial wastewater discharge leads to serious eutrophication of water bodies, but most of the adsorbents are difficult to selectively remove phosphorus and are difficult to use multiple times, therefore, developing an efficient and reusable material for removal phosphate is extremely necessary. In this work, a kind of highly selective phosphate adsorbent, microporous carbon material (MCM), based on glucose was synthesized by hydrothermal and activation method. The MCM were characterized by SEM, XPS, BET, element analysis, et al. The phosphate adsorption mechanism of MCM were investigated by batch adsorption experiment and model calculation. Results showed that MCM had a high adsorption capacity for phosphate in a wide range of pH (1.5-10). Langmuir model and pseudo-second-order kinetic revealed that the process was endothermic and involved both physical and chemical adsorption. The main phosphate adsorption mechanisms of MCM are electrostatic attraction, ion complexation, hydrogen bonding, and physical adsorption. The ions competition simulation experiment indicated that the MCM was highly selective for phosphate removal. Furthermore, the phosphate adsorption tests were carried out on five kinds of water, and the removal rates were all above 99.98%. The 20 regenerative cycles experiment revealed that the MCM had high reusability. Therefore, this kind of novel glucose-based highly selective phosphate adsorbent with multi-cycle phosphorus removal performance can improve the eutrophication of water. This study provides a new idea for phosphate removal and expands the application range of glucose-based carbon materials.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148452DOI Listing
June 2021

Ureteroscopic Cryoablation for Patients with Upper Tract Urothelial Carcinoma of a Solitary Kidney: A Porcine Model and Our Pilot Clinical Experience.

Ann Surg Oncol 2021 Jun 15. Epub 2021 Jun 15.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Purpose: To investigate the safety and efficacy of ureteroscopic cryoablation by a liquid-nitrogen system in a porcine model and for patients with upper tract urothelial carcinoma (UTUC) of a solitary kidney.

Methods: In the animal experiment, the right-sided ureter was frozen in nine pigs. Eight were randomly assigned to two different groups according to the freezing duration of 60 or 90 s. The other one was designed to receive a 10-min freeze. The treated ureters were harvested at 30 min, 2 days, 4 weeks, and 3 months after cryoablation for histological evaluation. After the animal study, we conducted a pilot clinical trial that enrolled six patients who were diagnosed with UTUC of a solitary kidney and received therapeutic management with ureteroscopic cryoablation at our center. Perioperative adverse events and oncological outcomes were evaluated.

Results: In the porcine model, the liquid-nitrogen system was capable of forming a therapeutic ice ball which infiltrated the full-thickness ureter and induced apoptosis and necrosis from mucosa to lamina muscularis through histological examination. In the clinical trial, cryoablation was successfully performed under ureteroscopy in all the patients, without intraoperative ureteral perforation, avulsion, or active hemorrhage. No recurrence in situ was observed during a median follow-up period of 12.5 months. Hydronephrosis and ureteral stricture was observed in one patient and was managed with ureteroscopic balloon dilation.

Conclusions: Ureteroscopic cryoablation induced by liquid nitrogen is a promising technique for conservative management of UTUC with benefits of improving local tumor control and preservation of a solitary kidney.
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http://dx.doi.org/10.1245/s10434-021-10233-5DOI Listing
June 2021

Multiomics analysis provides insights into alkali stress tolerance of sunflower (Helianthus annuus L.).

Plant Physiol Biochem 2021 May 29;166:66-77. Epub 2021 May 29.

Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, 130024, China. Electronic address:

Alkali stress is an extreme complex stress type, which exerts negative effects on plants via chemical destruction, osmotic stress, ion injury, nutrient deficiency, and oxygen deficiency. Soil alkalization has produced severe problems in some area, while plant alkali tolerance is poorly understood. Sunflower (Helianthus annuus L.) is an important oilseed crop with strong alkali tolerance. Here we exposed sunflower plants to alkali stress (NaHCO/NaCO = 9:1; pH 8.7) for whole life cycle. We applied transcriptomics, metabolomics, lipidomics and phytohormone analysis to elucidate the alkali tolerance mechanism of sunflower plant. Lipidomic analysis showed that alkali stress enhanced accumulation of saccharolipids and glycerolipids and lowered the accumulation of glycerophospholipids in sunflower seeds, indicating that alkali stress can change the lipid components of sunflower seeds, and that cultivating sunflower plants on alkalized farmlands will change the quality of sunflower seed oils. In addition, alkali stress downregulated expression of two rate-controlling genes of glycolysis in the leaves of sunflower but upregulated their expression in the roots. Enhanced glycolysis process provided more carbon sources and energy for alkali stress response of sunflower roots. Under alkali stress, accumulation of many fatty acids, amino acids, carbohydrates, and organic acids was greatly stimulated in sunflower roots. Alkali stress enhanced ACC, GA1, and ABA concentrations in the leaves but not in the roots, however, alkali stress elevated accumulation of BR (typhasterol) and CTK (Isopentenyladenosine) in the roots. We propose that multiple phytohormones and bioactive molecules interact to mediate alkali tolerance of sunflower.
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http://dx.doi.org/10.1016/j.plaphy.2021.05.032DOI Listing
May 2021

Arbuscular Mycorrhization Enhances Nitrogen, Phosphorus and Potassium Accumulation in by Modulating Soil Nutrient Balance under Elevated CO.

J Fungi (Basel) 2021 May 5;7(5). Epub 2021 May 5.

Centre of Excellence for Soil Biology, College of Resources and Environment, and Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400716, China.

Effects of arbuscular mycorrhizal fungi (AMF), elevated carbon dioxide (eCO), and their interaction on nutrient accumulation of leguminous plants and soil fertility is unknown. Plant growth, concentrations of tissue nitrogen (N), phosphorus (P), and potassium (K) in 12-week-old nodulated faba bean (, inoculated with bv. NM353), and nutrient use efficiency were thus assessed under ambient CO (410/460 ppm, daytime, 07:00 a.m.-19:00 p.m./nighttime, 19:00 p.m.-07:00 a.m.) and eCO (550/610 ppm) for 12 weeks with or without AM fungus of inoculation. eCO favored AMF root colonization and nodule biomass production. eCO significantly decreased shoot N, P and K concentrations, but generally increased tissue N, P and K accumulation and their use efficiency with an increased biomass production. Meanwhile, eCO enhanced C allocation into soil but showed no effects on soil available N, P, and K, while AM symbiosis increased accumulation of C, N, P, and K in both plant and soil though increased soil nutrient uptake under eCO. Moreover, plant acquisition of soil NO-N and NH-N respond differently to AMF and eCO treatments. As a result, the interaction between AM symbiosis and eCO did improve plant C accumulation and soil N, P, and K uptake, and an alternative fertilization for legume plantation should be therefore taken under upcoming atmosphere CO rising. Future eCO studies should employ multiple AMF species, with other beneficial fungal or bacterial species, to test their interactive effects on plant performance and soil nutrient availability in the field, under other global change events including warming and drought.
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http://dx.doi.org/10.3390/jof7050361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148158PMC
May 2021

Ganoderic Acid A Alleviates OVA-Induced Asthma in Mice.

Inflammation 2021 May 26. Epub 2021 May 26.

Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

The aim of this study is to investigate the effects of ganoderic acid A (GAA) on OVA-induced asthma in mice. Mouse asthma model was established by ovalbumin (OVA) in vitro. Diff-Quik staining was used to observe the total numbers of cells and the number of classification cells in each group, and HE staining was used to observe lung inflammation in lung tissue sections. ELISA was used to detect the effect of GAA on the levels of interleukin-4 (IL-4), IL-5, and IL-13 in serum and lung tissue. The expression levels of TLR/NF-κB were detected by Western blot. Immunohistochemistry was used to observe the expression changes of TLR4 and P-P65. Compared with the normal group, the inflammatory cell count, IL-4, IL-5, and IL-13 expression in the model group increased, and TLR/NF-kB signal protein expression increased. Compared with the model group, in GAA group, the number of inflammatory cells, the expression of IL-4, IL-5, and IL-13 decreased, and the expression of TLR/NF-kB signaling protein decreased. GAA regulated lung inflammation in asthmatic mice by inhibiting TLR/NF-kB signaling pathway.
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http://dx.doi.org/10.1007/s10753-021-01468-1DOI Listing
May 2021

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

J Clin Lab Anal 2021 Jul 21;35(7):e23822. Epub 2021 May 21.

Wenzhou Key Laboratory of Birth Defects, Wenzhou Central Hospital, Dingli Clinical Medical College of Wenzhou Medical University, Wenzhou, China.

Background: QRFPR is a recently identified member of the G protein-coupled receptor and is an orphan receptor for 26Rfa, which plays important role in the regulation of many physiological functions.

Methods: Here, we employed whole exome sequencing (WES) to examine the patients with intellectual disability (ID) and difficulty in feeding. We performed SIFT and PolyPhen2 predictions for the variants. The structure model was built from scratch by I-TASSER. Here, results derived from a number of cell-based functional assays, including shRNA experiment, intracellular Ca measurement, the expression of PI3 K-AKT-mTOR, and phosphorylation. The functional effect of QRFPR variants on PI3K-AKT-mTOR signaling was evaluated in vitro transfection experiments.

Result: Here, we identified two QRFPR variants at c.202 T>C (p.Y68H) and c.1111C>T (p.R371W) in 2 unrelated individuals. Structural analysis revealed that p.Y68H and p.R371W variants may affect the side chain structure of adjacent amino acids causing reduced binding of QRFPR to 26Rfa. The results show that QRFPR stimulated by 26Rfa leading to the transient rise of intracellular Ca . The QRFPR variations p.Y68H and p.R371 W can reduce the mobilization of intracellular Ca . The phosphorylation levels of the PI3K, Akt, and mTOR were significantly up- or downregulated by QRFPR overexpression or silencing, respectively. The QRFPR variations inhibited PI3K-AKT-mTOR signaling, resulting in downregulation of p-mTOR.

Conclusions: Our findings suggest that QRFPR acts as important role in neurodevelopment, and the effects of QRFPR are likely to be mediated by the Ca -dependent PI3K-AKT-mTOR pathways. Importantly, these findings provide a foundation for future elucidation of GPCR-mediated signaling and the physiological implications.
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http://dx.doi.org/10.1002/jcla.23822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275006PMC
July 2021

Ubiquitin-specific peptidase 22 in cancer.

Cancer Lett 2021 Aug 11;514:30-37. Epub 2021 May 11.

Department of Hepatobiliary and Pancreatic Surgery, The Center for Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China. Electronic address:

Recently, many studies have shown that deubiquitination modification of proteins is of great significance in major physiological processes such as cell proliferation, apoptosis, and differentiation. The ubiquitin-specific peptidase (USP) family is one of the most numerous and structurally diverse of the deubiquitinates known to date. USP22, an important member of the USP family, has been found to be closely associated with tumor cell cycle regulation, stemness maintenance, invasion and metastasis, chemoresistance, and immune regulation. We focus on recent advances regarding USP22's function in cancer and discuss the prospect of USP22 in this review.
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http://dx.doi.org/10.1016/j.canlet.2021.05.004DOI Listing
August 2021

Prenatal diagnosis of 7 cases with uniparental disomy by utilization of single nucleotide polymorphism array.

Mol Cytogenet 2021 Mar 19;14(1):19. Epub 2021 Mar 19.

Center of Prenatal Diagnosis, Wenzhou Central Hospital, Wenzhou, 325000, People's Republic of China.

Background: The phenotypes of uniparental disomy (UPD) are variable, which may either have no clinical impact, lead to clinical signs and symptoms. Molecular analysis is essential for making a correct diagnosis. This study involved a retrospective analysis of 4512 prenatal diagnosis samples and explored the molecular characteristics and prenatal phenotypes of UPD using a single nucleotide polymorphism (SNP) array.

Results: Out of the 4512 samples, a total of seven cases of UPD were detected with an overall frequency of 0.16%. Among the seven cases of UPD, two cases are associated with chromosomal aberrations (2/7), four cases (4/7) had abnormal ultrasonographic findings. One case presented with iso-UPD (14), and two case presented with mixed hetero/iso-UPD (15), which were confirmed by Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) as maternal UPD (15) associated with Prader-Willi syndrome (PWS). Four cases had iso-UPD for chromosome 1, 3, 14, and 16, respectively; this is consistent with the monosomy rescue mechanism. Another three cases presented with mixed hetero/isodisomy were consistent with a trisomy rescue mechanism.

Conclusion: The prenatal phenotypes of UPD are variable and molecular analysis is essential for making a correct diagnosis and genetic counselling of UPD. The SNP array is a useful genetic test in prenatal diagnosis cases with UPD.
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http://dx.doi.org/10.1186/s13039-021-00537-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980353PMC
March 2021

Three-Dimensional Maskless Fabrication of Bionic Unidirectional Liquid Spreading Surfaces Using a Phase Spatially Shaped Femtosecond Laser.

ACS Appl Mater Interfaces 2021 Mar 11;13(11):13781-13791. Epub 2021 Mar 11.

Laser Micro/Nano Fabrication Laboratory, School of Mechanical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.

Ubiquitous biological processes exhibit the ability to achieve spontaneous directionally guided droplet transport. Maskless three-dimensional (3D) fabrication of various miniature bionic structures, a method applicable to various materials, is subject to processing method limitations. This remains a large obstacle to realizing self-driven, continuous, and controllable unidirectional liquid spreading. Thus, we present a flexible maskless 3D method for fabricating bionic unidirectional liquid spreading surfaces by using a phase spatially shaped femtosecond laser. The laser can be transformed from having Gaussian distributions to having 3D bionic structure field distributions. Furthermore, we fabricated bionic structures with a spiculate end for unidirectional water spreading; 1 μL droplets had a 16 mm flow length on Si surfaces when the single structure was 34 (length), 8 (width), and 12 μm (height). Furthermore, various bionic structures-, cactus, and moth structures-were fabricated on Si, SiO, and Ti. We also demonstrated the measurability of two-dimensional (S-shaped) curved flows on Si wafers as well as 3D curved flows on a Ti pipe turning 120° within 2320 ms. Our method can realize high-efficiency maskless 3D processing of various materials and structures (especially asymmetric structures); it is both flexible and fast, effectively expanding the processing capacity of micro-/nanostructures on patterned surfaces. This is of great significance to various domains such as microfluids, fog collection, and chemical reaction control.
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http://dx.doi.org/10.1021/acsami.0c22080DOI Listing
March 2021

Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.

Mol Ther Nucleic Acids 2021 Mar 16;23:872-886. Epub 2021 Jan 16.

Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China.

Bladder cancer is a severe cancer with high mortality because of invasion and metastasis. Growing evidence has revealed that circular RNAs play critical roles in biological function, which is closely connected to proliferation and invasion of bladder cancer. In our study, we employed qRT-PCR, RNA fluorescence hybridization (FISH), 5-ethynyl-2'-deoxyuridine (EdU), CCK-8, Transwell assays, luciferase reporter assays, xenografts, and live imaging to detect the roles of circular RNA binding protein with multiple splicing (circRBPMS) in bladder cancer (BC). Bioinformatics analysis and WB were performed to investigate the regulatory mechanism. Expression profile analysis of circular RNAs (circRNAs) in BC revealed that circRBPMS was significantly downregulated. Low circRBPMS expression correlates with aggressive BC phenotypes, whereas upregulation of circRBPMS suppresses BC cell proliferation and metastasis by directly targeting the miR-330-3p/ retinoic acid induced 2 (RAI2) axis. miR-330-3p upregulation or silencing of RAI2 restored BC cell proliferation, invasion, and migration following overexpression of circRBPMS. RAI2 silencing reversed miR-330-3p-induced cell invasion and migration as well as growth inhibition . Moreover, through bioinformatic analysis of the downstream target of RAI2 in the TCGA database, we identified and validated the biological role of circRBPMS through the RAI2-mediated ERK and epithelial-mesenchymal transition (EMT) pathways. We summarize the circRBPMS/miR-330-3p/RAI2 axis, where circRBPMS acts as a tumor suppressor, and provide a potential biomarker and therapeutic target for BC.
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http://dx.doi.org/10.1016/j.omtn.2021.01.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868720PMC
March 2021

Daytime, Not Nighttime, Elevated Atmospheric Carbon Dioxide Exposure Improves Plant Growth and Leaf Quality of Mulberry ( L.) Seedlings.

Front Plant Sci 2020 4;11:609031. Epub 2021 Feb 4.

Centre of Excellence for Soil Biology, School of Resource and Environment, Southwest University, Chongqing, China.

Almost all elevated atmospheric CO concentrations (eCO) studies have not addressed the potential responses of plant growth to different CO in daytime and nighttime. The present study was to determine the impact of daytime and/or nighttime eCO on growth and quality of mulberry ( L.), a perennial multipurpose cash plant. Six-month-old mulberry seedlings were hence grown in environmentally auto-controlled growth chambers under four CO concentrations: (1) ambient CO (ACO, 410 μmol mol daytime/460 μmol mol nighttime), (2) sole daytime elevated CO (DeCO, 710 μmol mol/460 μmol mol), (3) sole nighttime elevated CO (NeCO, 410 μmol mol/760 μmol mol), and (4) continuous daytime and nighttime elevated CO (D + NeCO, 710 μmol mol/760 μmol mol). Plant growth characteristics, nutrient uptake, and leaf quality were then examined after 120 days of CO exposure. Compared to control, DeCO and (D + N)eCO increased plant biomass production and thus the harvest of nutrients and accumulation of leaf carbohydrates (starch, soluble sugar, and fatty acid) and N-containing compounds (free amino acid and protein), though there were some decreases in the concentration of leaf N, P, Mg, Fe, and Zn. NeCO had no significant effects on leaf yield but an extent positive effect on leaf nutritional quality due to their concentration increase in leaf B, Cu, starch, and soluble sugar. Meanwhile, (D + N)eCO decreased mulberry leaf yield and harvest of nutritious compounds for silkworm when compared with DeCO. The reason may be associated to N, P, Mg, Fe, and Zn that are closely related to leaf pigment and N metabolism. Therefore, the rational application of mineral nutrient (especially N, P, Fe, Mg, and Zn) fertilizers is important for a sustainable mulberry production under future atmosphere CO concentrations.
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http://dx.doi.org/10.3389/fpls.2020.609031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890035PMC
February 2021

Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.

Front Genet 2020 23;11:566918. Epub 2020 Dec 23.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Bladder carcinoma (BC) is one of the most prevalent and malignant tumors. Multiple gene signatures based on BC metabolism, especially regarding glycolysis, remain unclear. Thus, we developed a glycolysis-related gene signature to be used for BC prognosis prediction.

Methods: Transcriptomic and clinical data were divided into a training set and a validation set after they were downloaded and analyzed from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Gene-set enrichment analysis (GSEA) and differential analysis were used to screen differentially expressed genes (DEGs), while univariate Cox regression and lasso-penalized Cox regression were employed for signature establishment. To evaluate the prognostic power of the signature, receiver operating characteristic (ROC) curve and Kaplan-Meier (KM) survival analysis were also used. Additionally, we developed a nomogram to predict patients' survival chances using the identified prognostic gene signature. Further, gene mutation and protein expression, as well as the independence of signature genes, were also analyzed. Finally, we also performed qPCR and western blot to detect the expression and potential pathways of signature genes in BC samples.

Results: Ten genes were selected for signature construction among 71 DEGs, including nine risk genes and one protection gene. KM survival analysis revealed that the high-risk group had poor survival and the low-risk group had increased survival. ROC curve analysis and the nomogram validated the accurate prediction of survival using a gene signature composed of 10 glycolysis-related genes. Western blot and qPCR analysis demonstrated that the expression trend of signature genes was basically consistent with previous results. These 10 glycolysis-related genes were independent and suitable for a signature.

Conclusion: Our current study indicated that we successfully built and validated a novel 10-gene glycolysis-related signature for BC prognosis.
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http://dx.doi.org/10.3389/fgene.2020.566918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786194PMC
December 2020

Piperlongumine inhibits the growth of non-small cell lung cancer cells via the miR-34b-3p/TGFBR1 pathway.

BMC Complement Med Ther 2021 Jan 7;21(1):15. Epub 2021 Jan 7.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou City, 450052, Henan Province, China.

Background: Non-small cell lung cancer is a common type of lung cancer. Piperlongumine (PL), which is extracted from the roots of piperaceae plant, long pepper, and peppercorn, is an alkaloid amide that inhibits tumor growth and metastasis. However, whether it affects lung cancer cells remains unclear.

Methods: We assessed the effects of PL on the proliferation and apoptosis of A549 and H1299 NSCLC cell lines.

Results: PL was mildly toxic to normal human bronchial epithelial cells and significantly suppressed growth and facilitated apoptosis of A549 and H1299 cells. It also upregulated microRNA (miR)-34b-3p and downregulated the transforming growth factor beta type I receptor (TGFBR1). The dual-luciferase reporter assay showed that TGFBR1 is a target gene of miR-34b-3p. Silencing of miR-34b-3p or overexpression of TGFBR1 partially attenuated the effects of PL on A549 and H1299 cells.

Conclusions: PL inhibits proliferation and induces apoptosis of A549 and H1299 cells by upregulating miR-34b-3p and modulating TGFBR1 signaling pathway.
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http://dx.doi.org/10.1186/s12906-020-03123-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791704PMC
January 2021

Laser photonic-reduction stamping for graphene-based micro-supercapacitors ultrafast fabrication.

Nat Commun 2020 Dec 3;11(1):6185. Epub 2020 Dec 3.

Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.

Micro-supercapacitors are promising miniaturized energy storage devices that have attracted considerable research interest. However, their widespread use is limited by inefficient microfabrication technologies and their low energy density. Here, a flexible, designable micro-supercapacitor can be fabricated by a single pulse laser photonic-reduction stamping. A thousand spatially shaped laser pulses can be generated in one second, and over 30,000 micro-supercapacitors are produced within 10 minutes. The micro-supercapacitor and narrow gaps were dozens of microns and 500 nm, respectively. With the unique three-dimensional structure of laser-induced graphene based electrode, a single micro-supercapacitor exhibits an ultra-high energy density (0.23 Wh cm), an ultra-small time constant (0.01 ms), outstanding specific capacitance (128 mF cm and 426.7 F cm) and a long-term cyclability. The unique technique is desirable for a broad range of applications, which surmounts current limitations of high-throughput fabrication and low energy density of micro-supercapacitors.
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http://dx.doi.org/10.1038/s41467-020-19985-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712890PMC
December 2020

Corrigendum to "SNHG11 contributes to NSCLC cell growth and migration by targeting miR-485-5p/BSG axis" [Biomed. Pharmacother. 128 (2020) 110324].

Biomed Pharmacother 2020 Dec 2;132:110951. Epub 2020 Nov 2.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, Henan, China.

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http://dx.doi.org/10.1016/j.biopha.2020.110951DOI Listing
December 2020

Genetic analysis and prenatal diagnosis of 20 Chinese families with oculocutaneous albinism.

J Clin Lab Anal 2021 Feb 30;35(2):e23647. Epub 2020 Oct 30.

Key Laboratory of Birth Defects, Department of Genetics, Wenzhou Central Hospital, Wenzhou, China.

Background: Oculocutaneous albinism (OCA) is a group of heterogeneous genetic disorders characterized by abnormal melanin synthesis in the hair, skin, and eyes. OCA exhibits obvious genetic and phenotypic heterogeneity. Molecular diagnosis of causal genes can be of help in the classification of OCA subtypes and the study of OCA pathogenesis. METHODS: In this study, Sanger sequencing and whole exome sequencing were used to genetically diagnose 20 nonconsanguineous Chinese OCA patients. In addition, prenatal diagnosis was provided to six OCA families.

Results: Variants of TYR, OCA2, and HPS1 were detected in 85%, 10%, and 5% of affected patients, respectively. A total of 21 distinct variants of these three genes were identified. Exons 1 and 2 were the hotspot regions of the TYR variants, and c.895C > A and c.896G > A were the hotspot variants. We also found seven novel variants: c.731G > A, c.741C > A, c.867C > A, and c.1037-2A > T in TYR, c.695dupT and c.1054A > G in OCA2, and c.9C > A in HPS1. Genetic tests on six fetuses revealed three carrier fetuses, two normal fetuses, and one affected fetus. The follow-up results after birth were consistent with the results of prenatal diagnosis (one fetus terminated during pregnancy was not followed up).

Conclusions: This study expands our understanding of the genotypic spectrum of the Chinese OCA population. The findings indicate that prenatal diagnosis can provide important information for genetic counseling.
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http://dx.doi.org/10.1002/jcla.23647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891544PMC
February 2021

FGF18 Inhibits Clear Cell Renal Cell Carcinoma Proliferation and Invasion via Regulating Epithelial-Mesenchymal Transition.

Front Oncol 2020 29;10:1685. Epub 2020 Sep 29.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Fibroblast growth factor 18 (FGF18) is a member of the FGF family and contributes to a broad range of biological events. The important role of the overexpression of FGF18 has been identified in the progression of several types of cancers. However, there is still little information on the biological role of FGF18 on clear cell renal cell carcinoma (ccRCC), which is of interest in investigating the biological functions of FGF18 in ccRCC. Our results showed that FGF18 was lowly expressed in ccRCC tissues compared to paired normal renal tissue from the TCGA database and clinical cohort of Huashan Hospital and that high expression of FGF18 correlated with a good prognosis in ccRCC patients. In addition, overexpression of FGF18 significantly inhibited the proliferation ability of ccRCC cell lines and . Gene set enrichment analysis (GSEA) identified epithelial-mesenchymal transition (EMT) involved in a high FGF18 expression group of ccRCC patients in the TCGA cohort, which was further validated with EMT related markers in FGF18 overexpressed ccRCC cell lines. Furthermore, FGF18 overexpression significantly inhibited the PI3K/Akt pathway in ccRCC cells. Taken together, this study concludes that FGF18 is of potential value as a target for ccRCC.
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http://dx.doi.org/10.3389/fonc.2020.01685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552945PMC
September 2020

The correlation between the Th17/Treg cell balance and bone health.

Immun Ageing 2020 14;17:30. Epub 2020 Oct 14.

The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou, Jiangsu, 213003 China.

With the ageing of the world population, osteoporosis has become a problem affecting quality of life. According to the traditional view, the causes of osteoporosis mainly include endocrine disorders, metabolic disorders and mechanical factors. However, in recent years, the immune system and immune factors have been shown to play important roles in the occurrence and development of osteoporosis. Among these components, regulatory T (Treg) cells and T helper 17 (Th17) cells are crucial for maintaining bone homeostasis, especially osteoclast differentiation. Treg cells and Th17 cells originate from the same precursor cells, and their differentiation requires involvement of the TGF-β regulated signalling pathway. Treg cells and Th17 cells have opposite functions. Treg cells inhibit the differentiation of osteoclasts in vivo and in vitro, while Th17 cells promote the differentiation of osteoclasts. Therefore, understanding the balance between Treg cells and Th17 cells is anticipated to provide a new idea for the development of novel treatments for osteoporosis.
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http://dx.doi.org/10.1186/s12979-020-00202-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557094PMC
October 2020

Cytoplasmic Cargo Receptor p62 Inhibits Avibirnavirus Replication by Mediating Autophagic Degradation of Viral Protein VP2.

J Virol 2020 11 23;94(24). Epub 2020 Nov 23.

MOA Key Laboratory of Animal Virology, Center for Veterinary Sciences, Zhejiang University, Hangzhou, China

Selective autophagy regulates the degradation of cytoplasmic cargos, such as damaged organelles, invading pathogens, and aggregated proteins. Furthermore, autophagy is capable of degrading avibirnavirus, but the mechanism responsible for this process is unclear. Here, we show that autophagy cargo receptor p62 regulates the degradation of the avibirnavirus capsid protein VP2. Binding of p62 to VP2 enhances autophagic induction and promotes autophagic degradation of viral protein VP2. Further study showed that the interaction of p62 with viral protein VP2 is dependent on ubiquitination at the K411 site of VP2 and the ubiquitin-associated domain of p62. Mutation analysis showed that the K411R mutation of viral protein VP2 prohibits its p62-mediated degradation. Consistent with this finding, p62 lacking the ubiquitin-associated domain or the LC3-interacting region no longer promoted the degradation of VP2. Virus production revealed that the knockout of p62 but not the overexpression of p62 promotes the replication of avibirnavirus. Collectively, our findings suggest that p62 mediates selective autophagic degradation of avibirnavirus protein VP2 in a ubiquitin-dependent manner and is an inhibitor of avibirnavirus replication. Avibirnavirus causes severe immunosuppression and mortality in young chickens. VP2, the capsid protein of avibirnavirus, is responsible for virus assembly, maturation, and replication. Previous study showed that avibirnavirus particles could be engulfed into the autophagosome and degradation of virus particles took apart. Selective autophagy is a highly specific and regulated degradation pathway for the clearance of damaged or unwanted cytosolic components and superfluous organelles as well as invading microbes. However, whether and how selective autophagy removes avibirnavirus capsids is largely unknown. Here, we have shown that selective autophagy specifically clears ubiquitinated avibirnavirus protein VP2 by p62 recognition and that p62 is an inhibitor of avibirnavirus replication, highlighting the role of p62 as a potential drug target for mediating the removal of ubiquitinated virus components from cells.
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http://dx.doi.org/10.1128/JVI.01255-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925189PMC
November 2020

Development of a serum miRNA panel for detection of early stage non-small cell lung cancer.

Proc Natl Acad Sci U S A 2020 10 17;117(40):25036-25042. Epub 2020 Sep 17.

Institute of Cancer and Basic Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, China;

Minimally invasive testing for early detection of lung cancer to improve patient survival is a major unmet clinical need. This study aimed to develop and validate a serum multi-microRNA (multimiR) panel as a minimally invasive test for early detection of nonsmall cell lung cancer (NSCLC) regardless of smoking status, gender, and ethnicity. Our study included 744 NSCLC cases and 944 matched controls, including smokers and nonsmokers, male and female, with Asian and Caucasian subjects. Using RT-qPCR and a tightly controlled workflow, we quantified the absolute expression of 520 circulating microRNAs (miRNAs) in a Chinese cohort of 180 early stage NSCLC cases and 216 healthy controls (male smokers). Candidate biomarkers were verified in two case-control cohorts of 432 Chinese and 218 Caucasians, respectively (including females and nonsmokers). A multimiR panel for NSCLC detection was developed using a twofold cross-validation and validated in three additional Asian cohorts comprising 642 subjects. We discovered 35 candidate miRNA biomarkers, verified 22 of them, and developed a five-miR panel that detected NSCLC with area under curve (AUC) of 0.936-0.984 in the discovery and verification cohorts. The panel was validated in three independent cohorts with AUCs of 0.973, 0.916, and 0.917. The sensitivity of five-miR test was 81.3% for all stages, 82.9% for stages I and II, and 83.0% for stage I NSCLC, when the specificity is at 90.7%. We developed a minimally invasive five-miR serum test for detecting early stage NSCLC and validated its performance in multiple patient cohorts independent of smoking status, gender, and ethnicity.
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http://dx.doi.org/10.1073/pnas.2006212117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547174PMC
October 2020

Clinical application of chromosomal microarray analysis for fetuses with craniofacial malformations.

Mol Cytogenet 2020 25;13:38. Epub 2020 Aug 25.

Center of Prenatal Diagnosis, Wenzhou Central Hospital, Wenzhou, China.

Background: The potential correlations between chromosomal abnormalities and craniofacial malformations (CFMs) remain a challenge in prenatal diagnosis. This study aimed to evaluate 118 fetuses with CFMs by applying chromosomal microarray analysis (CMA) and G-banded chromosome analysis.

Results: Of the 118 cases in this study, 39.8% were isolated CFMs (47/118) whereas 60.2% were non-isolated CFMs (71/118). The detection rate of chromosomal abnormalities in non-isolated CFM fetuses was significantly higher than that in isolated CFM fetuses (26/71 vs. 7/47,  = 0.01). Compared to the 16 fetuses (16/104; 15.4%) with pathogenic chromosomal abnormalities detected by karyotype analysis, CMA identified a total of 33 fetuses (33/118; 28.0%) with clinically significant findings. These 33 fetuses included cases with aneuploidy abnormalities (14/118; 11.9%), microdeletion/microduplication syndromes (9/118; 7.6%), and other pathogenic copy number variations (CNVs) only (10/118; 8.5%).We further explored the CNV/phenotype correlation and found a series of clear or suspected dosage-sensitive CFM genes including , , , , , , , , , , and .

Conclusion: These findings enrich our understanding of the potential causative CNVs and genes in CFMs. Identification of the genetic basis of CFMs contributes to our understanding of their pathogenesis and allows detailed genetic counselling.
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http://dx.doi.org/10.1186/s13039-020-00502-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448974PMC
August 2020

Recent Advances in DNA Repair Pathway and Its Application in Personalized Care of Metastatic Castration-Resistant Prostate Cancer (mCRPC).

Methods Mol Biol 2020 ;2204:75-89

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Prostate cancer (PCa) is one of the common malignancies in male adults. In the era of precision medicine, many other novel agents targeting advanced prostate cancer, especially metastatic castration-resistant prostate cancer (mCRPC), are currently being evaluated. Among all these candidate therapies, poly-ADP ribose polymerase (PARP) inhibitors targeting DNA damage response (DDR) pathway has proven improving survival outcomes in clinical trials. In this review, we focus on recent advances in biology and clinical implication of DDR pathway and aim to discuss the latest results in advanced prostate cancer, especially mCRPC.
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http://dx.doi.org/10.1007/978-1-0716-0904-0_7DOI Listing
March 2021

SNHG11 contributes to NSCLC cell growth and migration by targeting miR-485-5p/BSG axis.

Biomed Pharmacother 2020 Aug 13;128:110324. Epub 2020 Jun 13.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Zhengzhou, 450052, Henan, China. Electronic address:

Accumulating studies implied that long noncoding RNAs (lncRNAs) act as essential factors in regulating diverse biological behaviors of cancers. Small nucleolar RNA host gene 11 (SNHG11) has been reported as for its oncogenic properties in several cancer types. However, it is unclear whether SNHG11 exerts functions in non-small cell lung cancer (NSCLC) remains unclear. The aim of this study was to inspect the role and regulatory mechanism of SNHG11 in NSCLC. The expression of SNHG11 in NSCLC cells was analyzed by qRT-PCR. Functional experiments were carried out to determine the effects of SNHG11 silence on the biological behaviors of NSCLC cells, including growth, migration and epithelial-mesenchymal transition. The inhibition of above functions was observed after SNHG11 was silenced. Subcellular fractionation and FISH assays were performed to detect the cellular distribution of SNHG11. Moreover, SNHG11 was found to be a sponge of miR-485-5p that could directly target to Basigin (BSG) mRNA. The interaction between SNHG11 and miR-485-5p as well as between miR-485-5p and BSG was proven by RNA pull down and luciferase reporter assays. Restoration assay confirmed the involvement of miR-485-5p and BSG in SNHG11-mediated NSCLC cellular functions. Conclusively, SNHG11 was overexpressed in NSCLC and functioned as a miR-485-5p sponge to up-regulate BSG.
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http://dx.doi.org/10.1016/j.biopha.2020.110324DOI Listing
August 2020

Molecular delineation of small supernumerary marker chromosomes using a single nucleotide polymorphism array.

Mol Cytogenet 2020 27;13:19. Epub 2020 May 27.

Center of Prenatal Diagnosis, Wenzhou Central Hospital, Wenzhou, 325000 People's Republic of China.

Background: Defining the phenotype-genotype correlation of small supernumerary marker chromosomes (sSMCs) remains a challenge in prenatal diagnosis. We karyotyped 20,481 amniotic fluid samples from pregnant women and explored the molecular characteristics of sSMCs using a single nucleotide polymorphism (SNP) array.

Results: Out of the 20,481 samples, 15 abnormal karyotypes with sSMC were detected (frequency: 0.073%) and the chromosomal origin was successfully identified by SNP array in 14 of them. The origin of sSMCs were mainly acrocentric-derived chromosomes and the Y chromosome. Two cases of sSMC combined with uniparental disomy (UPD) were detected, UPD(1) and UPD(22). More than half of the cases of sSMC involved mosaicism (8/15) and pathogenicity (9/15) in prenatal diagnosis. A higher prevalence of mosaicism for non-acrocentric chromosomes than acrocentric chromosomes was also revealed. One sSMC derived from chromosome 3 with a neocentromere revealed a 24.99-Mb pathogenic gain of the 3q26.31q29 region on the SNP array, which presented as an abnormal ultrasound indicating nasal bone hypoplasia.

Conclusion: The clinical phenotypes of sSMCs are variable and so further genetic testing and parental karyotype analysis are needed to confirm the characteristics of sSMCs. The SNP array used here allows a detailed characterisation of the sSMC and establishes a stronger genotype-phenotype correlation, thus allowing detailed genetic counselling for prenatal diagnosis.
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http://dx.doi.org/10.1186/s13039-020-00486-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251855PMC
May 2020

Prognostic values of YTHDF1 regulated negatively by mir-3436 in Glioma.

J Cell Mol Med 2020 07 25;24(13):7538-7549. Epub 2020 May 25.

Henan University, Kaifeng, Henan, P.R. China.

M6A methylation is likely to be closely associated with the occurrence and development of tumours. In this study, we demonstrated that the transcription levels of the m6A RNA methylation regulators are closely related to the prognosis of glioma. Univariate Cox analysis was performed on the expression levels of methylation regulators and selected three hub genes in glioma. Next, we systematically compared the expression of these m6A RNA methylation regulators in gliomas with different clinicopathological features. The overall survival (OS) curve of the hub genes was initially established based on TCGA database information. YTHDF1 was selected from the hub genes following survival and prognosis analysis. A nomogram was developed to predict the survival probability. We further performed cell function and in vivo xenograft tumour experiments to further verify its role in tumour progression. Next, based on the miRanda and miRDB databases, we predicted one microRNA, hsa-mir-346, that might regulate and bind to 3'UTR of YTHDF1, which was confirmed by our fluorescent enzyme reporter gene experiment. In summary, m6A RNA methylation regulators play a potential role in the progression of gliomas. YTHDF1 may have an essential function in glioma diagnosis, treatment and prognosis.
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http://dx.doi.org/10.1111/jcmm.15382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339155PMC
July 2020

Genetic risk scores based on risk-associated single nucleotide polymorphisms can reveal inherited risk of bladder cancer in Chinese population.

Medicine (Baltimore) 2020 May;99(19):e19980

Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, PR China.

Genome-wide association studies have identified single nucleotide polymorphisms (SNPs) associated with bladder cancer (BCa) risk in Caucasian and East Asian population. The objective of this study was to validate these SNPs in Chinese population and evaluate whether these SNPs could differentiate the individual inherited risk for BCa.A case-control study including 581 BCa cases and 1561 healthy controls was performed. Germline DNA samples from all individuals were genotyped for eight SNPs. Genetic risk score (GRS) was calculated for each individual based on the odds ratios and risk allele frequencies of five risk-associated SNPs.Among eight SNPs evaluated in this study, rs798766 at 4p16.3 [OR = 1.39 (1.15-1.67), P < .001], rs9642880 [OR = 1.17 (1.06-1.30), P < .001] and rs4813953 at 20p12.2 [OR = 1.09 (1.02-1.17), P = .016] were found associated with BCa risk in Chinese population. A genetic risk score was established based on five SNPs (including the above three SNPs and two other SNPs which have the consistent direction with previous reported genome-wide association study). The mean GRS was significantly higher in BCa cases than controls (1.22 vs. 1.01, P < .001). When subjects were categorized into low- (<0.8), average- (0.8-1.2), and high-risk (>1.2) groups, the likelihoods of BCa were 25.2%, 33.7% and 55.0%, respectively (P-trend < 2.2 × 10). In subgroup analyses, no significant difference was observed in mean GRS among BCa patients with different stages or grades.In conclusion, two SNPs derived from East Asian and one SNP from Caucasian were associated with BCa risk in Chinese population. These results provided additional information of genetic risks for BCa in Chinese population. Genetic risk score based on these SNPs can reveal inherited risk of BCa, and may have potential for modifying personalized cancer screening strategy.
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http://dx.doi.org/10.1097/MD.0000000000019980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220229PMC
May 2020

Non-disruptive mutation in DNA-binding domain is a beneficial factor of esophageal squamous cell carcinoma.

Ann Transl Med 2020 Mar;8(6):316

Institute of Cancer and Basic Medical (ICBM), Chinese Academy of Sciences, Hangzhou 310022, China.

Background: is frequently altered in esophageal squamous cell carcinoma (ESCC). However, the landscape of mutation and its effects on patients remain controversial.

Methods: Somatic mutations of in 161 patients with resectable ESCC were identified by next-generation sequencing (NGS) and verified by immunohistochemistry (IHC). Patients were stratified into seven mutations, and depending on the extent of the effect on the encoded protein, it was divided into "disruptive" and "non-disruptive" types. The association of mutation with clinicopathological properties and disease outcome was investigated.

Results: mutations were discovered in 85.7% patients, of which 68.9% carried mutations in the DNA-binding domain (DBD). A total of 47.8% and 37.9% patients had disruptive and non-disruptive mutations, respectively. Most patients carried only one mutation, but 15.5% had double mutations. mutations were dominant in exons 5 to 8. Missense mutation was the most frequent (97/163, 59.5%), and the top five frequently occurring variations included R273X, Y220X, H193, H179X, and R175H. Multivariable analysis revealed non-disruptive mutation in DBD as the independent prognostic predictor for progression-free survival (PFS) and overall survival (OS). The expression of p53 positively correlated with non-disruptive mutation in DBD. Patients with high p53 protein expression showed better outcomes.

Conclusions: Non-disruptive mutation in DBD serves as an independent beneficial prognostic factor of prolonged survival in resectable ESCC.
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http://dx.doi.org/10.21037/atm.2020.02.142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186752PMC
March 2020

Posteroanterior Lag Screws Versus Posterior Buttress Plate Fixation of Posterior Malleolar Fragments in Spiral Tibial Shaft Fracture.

J Foot Ankle Surg 2020 Jul - Aug;59(4):768-773. Epub 2020 Mar 29.

Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, P.R. China. Electronic address:

The aim of this study is to compare the clinical outcomes of posteroanterior (PA) lag screws versus posterior buttress plate fixation in treatment of posterior malleolar fragments (PMFs) in spiral tibial shaft fracture, and provide guidance for surgeons selecting a treatment strategy. A total of 48 eligible patients with PMFs associated with spiral tibial shaft fracture surgically treated from March 2009 to January 2016 were included in the study. They were divided into the screw group (n = 24) and the plate group (n = 24). All operations were performed via a posterolateral approach by a senior orthopedic surgeon. The American Orthopaedic Foot and Ankle Society (AOFAS) score, visual analog scale (VAS), and assessment of ankle range of motion (ROM) were used for clinical evaluation. The radiographic evaluation of posttraumatic arthritis scale was determined by Bargon reference criteria. At the mean follow-up period of 29.5 ± 4.3 and 30.4 ± 4.1 months, respectively (p > .05), all patients in both groups had bone union without severe wound problems or complications. There were no significant differences in AOFAS (92.5 ± 5.3 vs 94.7 ± 5.6, p = .129) and VAS (2.4 ± 0.8 vs 2.2 ± 0.9, p = .196) scores between the groups at final follow-up. No significant differences were found between the groups in injured/contralateral ankle ROM or posttraumatic ankle arthritis scale postoperatively (p > .05). For PMFs in spiral tibial shaft fracture, PA lag screws or posterior buttress plate fixation via a posterolateral approach can achieve good and equivalent clinical and radiological outcomes with minimal complications.
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http://dx.doi.org/10.1053/j.jfas.2019.09.039DOI Listing
June 2021