Publications by authors named "Chengyu Yang"

28 Publications

  • Page 1 of 1

Red Phosphorus Decorated TiO Nanorod Mediated Photodynamic and Photothermal Therapy for Renal Cell Carcinoma.

Small 2021 Jun 19:e2101837. Epub 2021 Jun 19.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.

Clear cell renal cell carcinoma (ccRCC) is a serious and tenacious disease. Photodynamic therapy (PDT) and photothermal therapy (PTT) are effective means of cancer treatment. However, PDT combined with PTT has been rarely reported in ccRCC treatment. In the present study, by developing the core-shell structured TiO @red phosphorus nanorods (TiO @RP NRs) as a photosensitizer, the feasibility and effectiveness of synchronous PDT and PTT treatments for ccRCC are demonstrated. The core-shell structured TiO @RP NRs are synthesized to drive the PDT and PTT for ccRCC, in which the RP shell is the sensitizer even in the near-infrared (NIR) region. The optimized TiO @RP NRs can respond to NIR and produce local heat under irradiation. The NRs are estimated in ccRCC treatments via cell counting kit-8 assay, propidium iodide staining, qRT-PCR, and reactive oxygen species (ROS) probes in vitro, while terminal deoxynucleotidyl transferase dUTP nick-end labeling is conducted in vivo. After NIR irradiation, TiO @RP NRs can efficiently kill ccRCC cells by producing local heat and ROS and cause low injury to normal kidney cells. Furthermore, treatment with TiO @RP NRs and NIR can kill significant numbers of deep-tissue ccRCC cells in vivo. This work highlights a promising photo-driven therapy for kidney cancer.
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http://dx.doi.org/10.1002/smll.202101837DOI Listing
June 2021

SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells.

Respir Res 2021 Jun 14;22(1):178. Epub 2021 Jun 14.

Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, China.

Background: Serine peptidase inhibitor, clade B, member 10 (SERPINB10) contributes to allergic inflammation in asthma. However, its role in the T-helper type 2 (Th2) response of allergic asthma is not known. The goal of this study was to unveil the function of SERPINB10 in the Th2 response of allergic asthma and the mechanism by which SERPINB10 affects the viability of Th2 cells.

Methods: Th2 cytokines and serum levels of house dust mite (HDM)-specific IgE in bronchoalveolar lavage fluid were examined by ELISA in an HDM-induced asthma model. The number and apoptosis of Th1 and Th2 cells in mouse lungs were measured by flow cytometry. Naïve CD4 T cells from patients with asthma were cultured under appropriate polarizing conditions to generate Th1 and Th2 cells. SERPINB10 expression in polarized Th1 and Th2 cells was quantified by real-time reverse transcription-quantitative polymerase chain reaction. SERPINB10 expression was knocked down in human CD4 T cells with lentivirus.

Results: Knockdown of SERPINB10 expression significantly diminished HDM-induced Th2 cytokine secretion and level of HDM-specific IgE. After HDM exposure, SERPINB10-knockdown mice had diminished numbers of Th2 cells, but similar numbers of Th1 cells, compared with those in negative-control mice. Th2 cells of SERPINB10-knockdown mice were more susceptible to apoptosis than that of control mice. Stimulating T-cell receptors (TCRs) with anti-CD3 antibody caused upregulation of SERPINB10 expression in polarized Th2 cells, but not polarized Th1 cells. Knockdown of SERPINB10 expression resulted in fewer numbers and greater apoptosis of polarized Th2 cells.

Conclusion: Our results suggest that SERPINB10 may contribute to allergic inflammation and the Th2 response of asthma by inhibiting the apoptosis of Th2 cells.
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http://dx.doi.org/10.1186/s12931-021-01757-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201873PMC
June 2021

Comment on "Microarray analysis of gene expression differences in microglia after exposure to graphene quantum dots".

Sci Total Environ 2021 06 11;773:146354. Epub 2021 Mar 11.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address:

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http://dx.doi.org/10.1016/j.scitotenv.2021.146354DOI Listing
June 2021

Design of a dispersion interferometer on a field-reversed configuration device.

Rev Sci Instrum 2021 Feb;92(2):023508

International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics, State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Dispersion interferometry (DI) is a promising method for density measurement. Compared with the traditional interferometer, the DI is immune to mechanical vibration and can avoid the fringe jump error. In addition, a simple optical configuration is also one of the advantages of the DI. The electron density of the Huazhong University of Science and Technology field-reversed configuration (HFRC) device can reach 10 m with a pulse length of 50 µs. In this case, the DI based on the CO laser on the HFRC device adopts the heterodyne technique based on the acousto-optic modulator, which can increase the temporal resolution to 40 MHz. It can realize density fluctuation measurements in the MHz range. The test of each optical element, especially the nonlinear crystal, has been completed. The AgGaSe crystal can produce a second harmonic wave of about 52.5 µW when the incident CO power is 10 W. Based on these designs and tests, a DI system can be expected on the HFRC device.
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http://dx.doi.org/10.1063/5.0040484DOI Listing
February 2021

Linarin Protects the Kidney against Ischemia/Reperfusion Injury via the Inhibition of Bioactive ETS2/IL-12.

Biol Pharm Bull 2021 ;44(1):25-31

Department of Nephrology, The Affiliated Hospital of Qingdao University.

Ischemia/reperfusion injury (IRI), a participant in acute kidney injury (AKI), can occur as a series of pathological processes such as inflammation. Linarin (LIN) has been widely used for different diseases. To confirm the anti-inflammatory value and relevant mechanism of LIN during IRI, in vivo and vitro models were established. LIN or dissolvent was given, and histologic analysis, quantitative (q)RT-PCR, serum creatinine and blood urea nitrogen testing were used to evaluate kidney injury. Microarray analysis, protein-protein interaction (PPI) analysis and molecular docking were used to identify the target protein of LIN, and small interfering RNA (siRNA) transfection was applied to explore the crucial role of identified protein. First, we found that LIN inhibited kidney injury in an in vivo IRI model and decreased the expression of interleukin-12 (IL-12) p40 in vivo and in vitro IRI models. To explore the mechanism of LIN, we collected raw data from a public microarray database and identified E26 oncogene homolog 2 (ETS2) as a crucial protein of LIN according to microarray analysis and PPI. Meanwhile, qRT-PCR indicated that IL-12 p40 showed no significant difference between ETS2 knock down group and LIN treated ETS2 knock down group after hypoxia reoxygenation treatment. In addition, according to molecular docking the contact area is highly conserved and located on a PPI domain of ETS2 which indicates that LIN may alter the interaction with synergistic proteins in the regulation of IL-12 p40 expression. Our study demonstrated the anti-inflammatory effect of LIN during IRI-AKI, broadening the medicinal value of LIN and the therapeutic options for IRI-AKI.
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http://dx.doi.org/10.1248/bpb.b20-00508DOI Listing
January 2021

Nicotiflorin attenuates cell apoptosis in renal ischemia-reperfusion injury through activating transcription factor 3.

Nephrology (Carlton) 2021 Apr 25;26(4):358-368. Epub 2021 Feb 25.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Introduction: Nicotiflorin is the main characteristic component of Nymphaea candida, which is a natural product that reportedly ameliorates acute injury of the liver and cerebral cortex, but the effect of nicotiflorin on acute kidney injury (AKI) remains unknown. This study aimed to investigate the effects of nicotiflorin on ischaemia/reperfusion (I/R) AKI and the associated mechanisms.

Methods: We performed both (a) in vivo experiments with C57BL/6 mice with bilateral renal pedicles clamped for 45 minutes and (b) in vitro experiments with human kidney epithelial cells (HK-2) exposed to hypoxia/reoxygenation to mimic I/R injury to study the role of nicotiflorin in AKI.

Results: In vivo, nicotiflorin administration exerted protective effects on renal injury, as demonstrated by reductions in the levels of caspase3 and Bad (P < .05), the upregulation of Bcl-2 expression (P < .05) and improved renal histologic changes, which suggested that nicotiflorin can alleviate I/R injury and cell apoptosis. In vitro, nicotiflorin at a concentration of 75 μg/mL protected cells from hypoxia, which further confirmed that nicotiflorin exerts beneficial effects on hypoxia/reoxygenation. Through computational molecular docking, we found that activating transcription factor 3 (ATF3) exhibits a robust interaction with nicotiflorin with a simulated binding energy of -9.2°. We verified the interaction of nicotiflorin with ATF3 in HK-2 cells, and found that nicotiflorin reduced the apoptosis of HK-2 through ATF3.

Conclusion: Based on the above-described results, nicotiflorin appears to have a beneficial impact on deteriorated renal function, as demonstrated using an experimental I/R model. The underlying mechanisms of nicotiflorin might inhibit HK-2 cell apoptosis through ATF3.
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http://dx.doi.org/10.1111/nep.13841DOI Listing
April 2021

Scutellarin Ameliorates Renal Injury via Increasing CCN1 Expression and Suppressing NLRP3 Inflammasome Activation in Hyperuricemic Mice.

Front Pharmacol 2020 22;11:584942. Epub 2020 Oct 22.

The Affiliated Hospital of Qingdao University, Qingdao, China.

Considerable evidences have indicated that elevated uric acid (UA) was involved in renal tubular injury leading to hyperuricemic nephropathy (HN). Scutellarin is a biologically active flavonoid derived from the Chinese traditional herb Hand-Mazz, which has been widely used in the treatment of cardiovascular and cerebrovascular diseases. In the present study, we analyzed the effect of scutellarin on HN, by using C57BL/6 mice and human renal tubular epithelial cell line HK-2 which was subjected to adenine/potassium oxonate and UA to mimic a HN injury. The HN mice showed a significant decrease in renal function with the increased SCr and blood urea nitrogen (BUN) ( < 0.05). Hematoxylin-eosin staining results showed a histological injury in HN mice kidney tissues with severe tubular damage. Scutellarin dose dependently alleviated the renal injury of the HN model ( < 0.05), and a dose of 20 mg/kg/day remarkably reduced the Scr level (26.10 ± 3.23 μmol/ml vs. 48.39 ± 7.51 μmol/ml, < 0.05) and BUN (151.12 ± 30.24 mmol/L vs. 210.43 ± 45.67 mmol/L, < 0.05) compared with the HN model group. Similarly, scutellarin decreased NGAL, Kim-1, cystatin C, and IL-18 protein expression levels in HN mouse ( < 0.05). Overexpressed CCN1 could not induce NLRP3 inflammasome activation, with no change of mRNA and protein expression levels of NLRP3, ASC, and pro-caspase-1 compared with the control HK-2. However, HK-2 showed a significant NLRP3 inflammasome activation and apoptosis. Importantly, knockdown of CCN1 not only aggravated NLRP3 inflammasome activation and apoptosis but also abrogated the protective effect of scutellarin in UA-induced HK-2 injury. Thus, scutellarin might alleviate HN progression a mechanism involved in CCN1 regulation on NLRP3 inflammasome activation.
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http://dx.doi.org/10.3389/fphar.2020.584942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641948PMC
October 2020

Possible Novel Therapeutic Targets in Lymphangioleiomyomatosis Treatment.

Front Med (Lausanne) 2020 24;7:554134. Epub 2020 Sep 24.

Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Lymphangioleiomyomatosis (LAM) is a rare systemic neoplastic disease that exclusively happens in women. Studies focusing on LAM and tuberous sclerosis complex (TSC) have made great progress in understanding the pathogenesis and searching for treatment. The inactive mutation of TSC1 or TSC2 is found in patients with LAM to activate the crucial mammalian target of rapamycin (mTOR) signaling pathway and result in enhanced cell proliferation and migration. However, it does not explain every step of tumorigenesis in LAM. Because cessation of rapamycin would break the stabilization of lung function or improved quality of life and lead to disease recurrent, continued studies on the pathogenesis of LAM are necessary to identify novel targets and new treatment. Researchers have found several aberrant regulations that affect the mTOR pathway such as its upstream or downstream molecules and compensatory pathways in LAM. Some therapeutic targets have been under study in clinical trials. New methods like genome-wide association studies have located a novel gene related to LAM. Herein, we review the current knowledge regarding pathogenesis and treatment of LAM and summarize novel targets of therapeutic potential recently.
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http://dx.doi.org/10.3389/fmed.2020.554134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542236PMC
September 2020

Construction of [email protected]α-glucosidase magnetic nanoparticles for ligand fishing of α-glucosidase inhibitors from a natural tonic Epimedii Folium.

Int J Biol Macromol 2020 Dec 10;165(Pt A):1361-1372. Epub 2020 Oct 10.

School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China. Electronic address:

Inhibition of α-glucosidase activity is an effective way for treatment of type 2 diabetes mellitus. Epimedii Folium is an important source of α-glucosidase inhibitors (AGIs), however bioactive compounds and pharmacological mechanisms remained unclear. In this study, a novel strategy was established, which harnessed α-glucosidase functionalized magnetic beads to fish out potential AGIs, followed by UPLC-MS/MS analysis for their identification. Furthermore, molecular docking was employed to predict binding patterns between the AGIs and the enzyme, and IC values was estimated as well. After response surface methodology optimization, the highest activity of [email protected]α-glucosidase has been achieved when 1.17 mg/mL of α-glucosidase was immobilized in phosphate buffer (pH 6.81) for 4.22 h. Moreover, eight flavonoids were fished out from the extract of Epimedii Folium, and then identified to be epimedin A, epimedin B, epimedin C, icariin, sagittatoside A, sagittatoside B, 2"-O-rhamnosyl icariside II and baohuoside I. All of them were further confirmed to be AGIs through in vitro inhibitory assay and molecular docking. Among those, baohuoside I and sagittatoside B possessed stronger inhibitory activity than acarbose. The approach has a significant prospect in conveniently screening bioactive compounds that target various receptors, which provided an efficient platform for new drug development from natural products.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.018DOI Listing
December 2020

MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.

Front Physiol 2020 27;11:510. Epub 2020 May 27.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Renal tubular epithelial cell (RTEC) death and renal interstitial inflammation are the most crucial pathophysiological changes in acute kidney ischemia/reperfusion injury (IRI). The microRNA (miR)-181d family plays diverse roles in cell proliferation, apoptosis and inflammation, but its renal target and potential role in IRI are unknown. Here, we showed that the expression of miR-181d-5p decreased and Krueppel-like factor 6 (KLF6) increased in a renal cell (HK-2) model of hypoxia/reoxygenation (H/R) injury and a mouse model of renal IRI. They were mainly distributed in the renal tubules. After renal IRI, miR-181d-5p overexpression significantly inhibited inflammatory mediators, reduced apoptosis and further improved renal function. KLF6 exacerbated RTEC damage and acted as a NF-κB co-activator to aggravate the renal IRI inflammatory response. Mechanistically, KLF6 was predicted as a new potential target gene of miR-181d-5p through bioinformatic analysis and luciferase reporter assay verification. After overexpressing miR-181d-5p and inhibiting KLF6, the role of miR-181d-5p was weakened on the renal damage improvement. In conclusion, miR-181d-5p upregulation produced protective antiapoptotic and anti-inflammatory effects against IRI in kidneys and H/R injury in HK-2 cells , and these effects were achieved by targeted inhibition of KLF6. Thus, our results provide novel insights into the molecular mechanisms associated with IRI and a potential novel therapeutic target.
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http://dx.doi.org/10.3389/fphys.2020.00510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295155PMC
May 2020

Comment on 'Comparison of the toxic mechanism of T-2 toxin and deoxynivalenol on human chondrocytes by microarray and bioinformatics analysis'.

Toxicol Lett 2020 07 23;327. Epub 2020 Mar 23.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address:

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http://dx.doi.org/10.1016/j.toxlet.2020.03.015DOI Listing
July 2020

Comment on 'Di-(2-ethylhexyl)-phthalate induces apoptosis via the PPARγ/PTEN/AKT pathway in differentiated human embryonic stem cells'.

Food Chem Toxicol 2020 05 18;139:111281. Epub 2020 Mar 18.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address:

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http://dx.doi.org/10.1016/j.fct.2020.111281DOI Listing
May 2020

Salidroside suppresses group 2 innate lymphoid cell-mediated allergic airway inflammation by targeting IL-33/ST2 axis.

Int Immunopharmacol 2020 Apr 15;81:106243. Epub 2020 Feb 15.

Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address:

Salidroside, an active component extracted from Rhodiola rosea, has been reported to inhibit allergic asthma. However, its mechanism has not been fully elucidated. Group 2 innate lymphoid cells (ILC2s) accumulate in the lung and cooperate with other cells to drive type 2 inflammation stimulated by inhaled allergens. The study aims to explore the suppressive effect of salidroside on ILC2s and IL-33/IL-33R (ST2) axis in allergic airway inflammation. The ovalbumin (OVA)-sensitized/challenged mice were established. Airway eosinophil recruitment, increased total IgE in the serum and type 2 cytokines IL-4, IL-5, and IL-13 in the bronchoalveolar lavage fluids and lung tissues were identified in the OVA-induced mice model, all of which were inhibited by pretreatment with different doses of salidroside. Moreover, salidroside suppressed lung total ILC2 and ST2-expressing ILC2 accumulation, lung IL-33 and ST2 expressions in mice. In vitro, OVA could induce IL-33 expression in BEAS-2B cells, which was also effectively inhibited by salidroside. This study firstly reveals salidroside as a potential therapeutic drug for allergic asthma by inhibiting ILC2-mediated airway inflammation via targeting IL-33/ST2 axis.
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http://dx.doi.org/10.1016/j.intimp.2020.106243DOI Listing
April 2020

A new promising Ni-MOF superstructure for high-performance supercapacitors.

Chem Commun (Camb) 2020 Feb 17;56(12):1803-1806. Epub 2020 Jan 17.

Fujian Provincial Key Laboratory of Electrochemical Energy Storage Materials, Fuzhou University, Fuzhou, Fujian 350116, China. and Jiangsu Collaborative Innovation Center of Photovolatic Science and Engineering, Changzhou University, Changzhou, Jiangsu 213164, China.

A new layered structural Ni-MOF nanosheet has been successfully synthesized and used for the first time as an electrode for a supercapacitor and a flexible all-solid-state asymmetric supercapacitor, exhibiting capacitances of 1518.8 F g at 1 A g and 244 mF cm at 0.5 mA cm, respectively.
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http://dx.doi.org/10.1039/c9cc09302hDOI Listing
February 2020

Comment on 'endoplasmic reticulum stress mediates inflammatory response triggered by ultra-small superparamagnetic iron oxide nanoparticles in hepatocytes'.

Nanotoxicology 2020 05 8;14(4):533. Epub 2020 Jan 8.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

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http://dx.doi.org/10.1080/17435390.2019.1710871DOI Listing
May 2020

Comment on 'miR-338-5p inhibits cell proliferation, colony formation, migration, and cisplatin resistance in esophageal squamous cancer cells by targeting FERMT2'.

Carcinogenesis 2020 04;41(2):246

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

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http://dx.doi.org/10.1093/carcin/bgz089DOI Listing
April 2020

Fabrication and characterization of a high performance polyimide ultrafiltration membrane for dye removal.

J Colloid Interface Sci 2020 Mar 19;562:589-597. Epub 2019 Nov 19.

Center for Membrane Separation and Water Science & Technology, Ocean College, Zhejiang University of Technology, Hangzhou 310014, PR China.

Membrane separation technology is one of the cost effective and most efficient technologies for treatment of wastewater from textile industry. However, development of membranes with better performance and thermal stability is still a highly challenging task. In this study, successful preparation of a novel thermally stable polyimide (PI) polymer was demonstrated using 2,4,6-trimethyl-1,3-phenylenediamine, 4,4'-diaminodiphenylmethane and 1,2,4,5-benzenetetracarboxylic dianhydride components. PI was selected as representative candidate because of its excellent thermal stability (decomposition temperature of 529 °C), as revealed by thermogravimetric analysis. Furthermore, PI polymer was used to fabricate ultrafiltration (UF) membrane by phase inversion process. This UF membrane is especially interesting as it allowed for almost complete penetration of monovalent (NaCl) and divalent (NaSO) inorganic salts because of its molecular weight cut off of 9320 Da. Moreover, the membrane exhibited very good surface hydrophilicity with the water contact angle of 67.6°. This PI-based UF membrane was found to be substantially effective as it showed high pure-water and dye-permeation fluxes of 345.10 and 305.58 L m h at 0.1 MPa, respectively. Besides, the membrane exhibited a rejection of 98.65% toward the direct red 23 dye (100 ppm) at 0.1 MPa. Thus, this PI-based UF membrane is highly beneficial and acts as a potential candidate for dye removal from wastewater produced by textile industry.
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http://dx.doi.org/10.1016/j.jcis.2019.11.075DOI Listing
March 2020

Comment on "Transcriptomic response of exposed to hydroxylated polychlorinated biphenyls (OH-PCBs)".

Int J Phytoremediation 2020 25;22(2):223. Epub 2019 Jul 25.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

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http://dx.doi.org/10.1080/15226514.2019.1645089DOI Listing
January 2020

Letter to the Editor: "Integrative Genomic Analysis of OCT1 Reveals Coordinated Regulation of Androgen Receptor in Advanced Prostate Cancer".

Endocrinology 2019 05;160(5):1065

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

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http://dx.doi.org/10.1210/en.2019-00138DOI Listing
May 2019

Comment on "Nuclear localization of LDL receptor-related protein 1B in mammary gland carcinogenesis".

J Mol Med (Berl) 2019 05 15;97(5):737. Epub 2019 Mar 15.

Department of Nephrology, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China.

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http://dx.doi.org/10.1007/s00109-019-01773-1DOI Listing
May 2019

Adaptive evolution and carbon dioxide fixation of Chlorella sp. in simulated flue gas.

Sci Total Environ 2019 Feb 6;650(Pt 2):2931-2938. Epub 2018 Oct 6.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.

Carbon dioxide and other greenhouse gas emissions leads to global warming. Biological capture through microalgae is a potential approach for solving this environmental problem. It is still a technical challenge to enhance the tolerance of microalgae to flue gas if CO is fixed from flue gas directly. A new strain, Chlorella sp. Cv was obtained through adaptive evolution (46 cycles) against simulated flue gas (10% CO, 200 ppm NO and 100 ppm SO). It was confirmed that Chlorella sp. Cv could tolerate simulated flue gas conditions and the maximum CO fixation rate was 1.2 g L d. In a two-stage process, the biomass concentration was 2.7 g L and the carbohydrate content was 68.4%. Comparative transcriptomic analysis was performed for Chlorella sp. Cv under simulated flue gas and control conditions (10% CO). These responses against simulated flue gas uncovered the significant difference between the evolved strain and the original strain. The metabolic responses to flue gas were explored with focus on various specific genes. Upregulation of several genes related to photosynthesis, oxidative phosphorylation, CO fixation, sulfur metabolism and nitrogen metabolism was beneficial for the evolved strain to tolerate the simulated flue gas. The upregulation of genes related to extracellular sulfur transport and nitrate reductase was essential to utilize the sulfate and nitrate from dissolved SO and NO. The results in this study are helpful to establish a new process for CO capture directly from industrial flue gas.
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http://dx.doi.org/10.1016/j.scitotenv.2018.10.070DOI Listing
February 2019

Novel THRB mutation analysis in congenital hypothyroidism with thyroid dysgenesis.

J Cell Biochem 2018 11 3;119(11):9474-9482. Epub 2018 Aug 3.

Prenatal Diagnosis Center, Affiliated Hospital of Qingdao University, Qingdao, China.

Thyroid dysgenesis (TD) accounts for most cases of congenital hypothyroidism. Although mutations in thyroid hormone receptor β (THRB) have been identified in TD, the mutational spectrum of THRB and phenotype-genotype correlations have not been fully elucidated. In this study, we aimed to find mutations of THRB, examine the functions of these mutations, and attempt to elucidate the relationship between THRB and TD. Thus, we screened the exons of THRB in 280 patients with TD and 200 normal subjects in samples collected from China. We performed cell morphology assays, MTT assays, flow cytometric analyses, and a quantitative reverse-transcription polymerase chain reaction in human thyroid follicular epithelial cells (Nthy-ori cell line) to examine the impact of THRB mutations. In two unrelated patients, two novel missense mutations, c.76G>A (p.D26N) and c.107G>A (p.C36Y), were identified in THRB. Functional studies suggested that the C36Y mutant caused changes in morphology, inhibiting cell proliferation and promoting apoptosis in a human thyroid cell line. In addition, we found that messenger RNA expressions of thyroglobulin (TG) and the Na /I symporter (NIS) were decreased in a time-dependent manner in mutant THRB compared with the wild type. To our knowledge, this is the first study to document the prevalence of THRB mutations and the genotype-phenotype spectrum of TD in a Chinese population. We characterized the function of a C36Y mutation, which reduced cell proliferation and increased cell death in thyroid epithelial cells. This study provides further evidence for genetic THRB defects and disease mechanisms in TD.
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http://dx.doi.org/10.1002/jcb.27264DOI Listing
November 2018

A new genetic mechanism of natural gas accumulation.

Sci Rep 2018 05 29;8(1):8336. Epub 2018 May 29.

Exploration and Development Research Institute of Southwest Oil & Gasfield Company, PetroChina, Chengdu, Sichuan, 610041, China.

Natural gas of organic origin is primarily biogenic or thermogenic; however, the formation of natural gas is occasionally attributed to hydrothermal activity. The Precambrian dolomite reservoir of the Anyue gas field is divided into three stages. Dolomite-quartz veins were precipitated after two earlier stages of dolomite deposition. Fluid inclusions in the dolomite and quartz are divided into pure methane (P-type), methane-bearing (M-type), aqueous (W-type), and solid bitumen-bearing (S-type) inclusions. The W-type inclusions within the quartz and buried dolomite homogenized between 107 °C and 223 °C. Furthermore, the trapping temperatures and pressures of the fluid (249 °C to 319 °C and 1619 bar to 2300 bar, respectively) are obtained from the intersections of the isochores of the P-type and the coeval W-type inclusions in the quartz. However, the burial history of the reservoir indicates that the maximum burial temperature did not exceed 230 °C. Thus, the generation of the natural gas was not caused solely by the burial of the dolomite reservoir. The results are also supported by the presence of paragenetic pyrobitumen and MVT lead-zinc ore. A coupled system of occasional invasion by hydrothermal fluids and burial of the reservoir may represent a new genetic model for natural gas accumulation in this gas field.
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http://dx.doi.org/10.1038/s41598-018-26517-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974082PMC
May 2018

An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance.

Chem Sci 2017 Dec 4;8(12):8012-8018. Epub 2017 Oct 4.

College of Pharmaceutical Sciences , Zhejiang University , Hangzhou 310058 , China . Email:

Unveiling the detailed roles of glutathione (GSH) in chemoresistance necessitates a reliable assay for its detection in intact live specimens. Herein, by taking advantage of the susceptibility of electron-poor C -S bond to GSH nucleophilic attack, we developed a naphthalimide-sulfoxide based fluorogenic probe () applicable for tracking endogenous GSH fluctuation in live cells. features a high degree of sensitivity towards GSH as demonstrated by its utmost 2200-fold fluorogenic response. As a proof of concept, has been applied to image GSH in liver cancer HepG2 cells with the normal L02 cell counterparts serving as a control, and elevated GSH level was observed in HepG2 in contrast to L02. Further experiments showed that this elevated GSH level was involved in doxorubicin-resistance but not in cisplatin-resistance. Noteworthy, monitoring GSH change in HepG2 and L02 cells in response to doxorubicin treatment revealed that while normal cells showed a burst of GSH in adaption to doxorubicin treatment, no significant change was detected in HepG2 cells, suggesting that HepG2 cells have been preconditioned by their intrinsic oxidative stress which confers drug-resistance. Given the observed sensitivity and spatiotemporal resolution, should hold promise for future study on the detailed roles of GSH in drug-resistance.
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http://dx.doi.org/10.1039/c7sc03338aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853925PMC
December 2017

Pore structure characterization of Chang-7 tight sandstone using MICP combined with NGA techniques and its geological control factors.

Sci Rep 2016 11 10;6:36919. Epub 2016 Nov 10.

Strategic Research Center of Oil and Gas Resource, Ministry of Land and Resources, Beijing 100034 PR, China.

Understanding the pore networks of unconventional tight reservoirs such as tight sandstones and shales is crucial for extracting oil/gas from such reservoirs. Mercury injection capillary pressure (MICP) and N gas adsorption (NGA) are performed to evaluate pore structure of Chang-7 tight sandstone. Thin section observation, scanning electron microscope, grain size analysis, mineral composition analysis, and porosity measurement are applied to investigate geological control factors of pore structure. Grain size is positively correlated with detrital mineral content and grain size standard deviation while negatively related to clay content. Detrital mineral content and grain size are positively correlated with porosity, pore throat radius and withdrawal efficiency and negatively related to capillary pressure and pore-to-throat size ratio; while interstitial material is negatively correlated with above mentioned factors. Well sorted sediments with high debris usually possess strong compaction resistance to preserve original pores. Although many inter-crystalline pores are produced in clay minerals, this type of pores is not the most important contributor to porosity. Besides this, pore shape determined by NGA hysteresis loop is consistent with SEM observation on clay inter-crystalline pores while BJH pore volume is positively related with clay content, suggesting NGA is suitable for describing clay inter-crystalline pores in tight sandstones.
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http://dx.doi.org/10.1038/srep36919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103269PMC
November 2016

Research on structural design and test technologies for a three-chamber launching device.

Rev Sci Instrum 2016 Jul;87(7):075107

Luoyang Hydraulic Engineering Technical Research Institute, Luoyang, Henan 471023, China.

A three-chamber launching device with improved acceleration is proposed and developed. As indicated by the damage generated during the pill and engineering protection tests, the proposed device is applicable as a high-speed launching platform for pills of different shapes and quality levels. Specifically, it can be used to investigate kinetic energy weapons and their highly destructive effects due to the resulting large bomb fragments. In the horizontal direction of the barrel, two auxiliary chambers are set at a certain distance from the main chamber. When the pill reaches the mouth of the auxiliary chambers, the charges in the auxiliary chambers are ignited by the high-temperature, high-pressure combustible gas trailing the pill. The combustible gas in the auxiliary chambers can resist the rear pressure of the pill and thus maintain the high pressure of the pill base. In this way, the required secondary acceleration of the pill is met. The proposed device features the advantage of launching a pill with high initial velocity under low bore pressure. Key techniques are proposed in the design of the device to address the problems related to the angle between the main chamber axis and the ancillary chamber axis, the overall design of a three-chamber barrel, the structural design of auxiliary propellant charge, the high-pressure combustible gas sealing technology, and the sabot and belt design. Results from the launching test verify the reasonable design of this device and its reliable structural sealing. Additionally, the stiffness and the strength of the barrel meet design requirements. Compared with the single-chamber launching device with the same caliber, the proposed device increases the average launching velocity by approximately 15% and the amount of muzzle kinetic energy by approximately 35%. Therefore, this equipment is capable of carrying out small-caliber, high-speed pill firing tests.
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http://dx.doi.org/10.1063/1.4958650DOI Listing
July 2016

The short- and long-term effects of orally administered high-dose reduced graphene oxide nanosheets on mouse behaviors.

Biomaterials 2015 Nov 1;68:100-13. Epub 2015 Aug 1.

Research Center for Translational Medicine at Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, PR China.

Reduced graphene oxide (rGO), a carbon-based nanomaterial, has enormous potential in biomedical research, including in vivo cancer therapeutics. Concerns over the toxicity remain outstanding and must be investigated before clinical application. The effect of rGO exposure on animal behaviors, such as learning and memory abilities, has not been clarified. Herein, we explored the short- and long-term effects of orally administered rGO on mouse behaviors, including general locomotor activity level, balance and neuromuscular coordination, exploratory and anxiety behaviors, and learning and memory abilities using open-field, rotarod, and Morris water maze tests. Compared with mice administered buffer-dispersed mouse chow or buffer alone, mice receiving a high dose of small or large rGO nanosheets showed little change in exploratory, anxiety-like, or learning and memory behaviors, although general locomotor activity, balance, and neuromuscular coordination were initially affected, which the mechanisms (e.g. the influence of rGO exposure on the activity of superoxide dismutase in mouse serum) were discussed. The results presented in this work look to provide a deep understanding of the in vivo toxicity of rGO to animals, especially its effect on learning and memory and other behaviors.
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http://dx.doi.org/10.1016/j.biomaterials.2015.07.060DOI Listing
November 2015

A highly specific probe for sensing hydrogen sulfide in live cells based on copper-initiated fluorogen with aggregation-induced emission characteristics.

Theranostics 2014 24;4(12):1233-8. Epub 2014 Sep 24.

3. Division of Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital and Department of Radiology, Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.

Here we reported the first fluorescent probe with aggregation-induced emission characteristics, namely AIE-S, for the detection of hydrogen sulfide (H2S) in live cells. The detection system is selective for complicated biological application and the response is fast enough to complete within seconds. Moreover, the probe exhibits the unique advantage of being immune to aggregation-caused quenching which is a detrimental phenomenon limiting the application of most current available H2S fluorescent probes. The detection mechanism was investigated and postulated to be S(2-) initiated de-coordination and thereafter aggregation of the AIE-S complex.
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http://dx.doi.org/10.7150/thno.10330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184000PMC
June 2015