Publications by authors named "Chenglin Hong"

16 Publications

  • Page 1 of 1

In-vitro and in-vivo monitoring of gold(III) ions from intermediate metabolite of sodium aurothiomalate through water-soluble ruthenium (II) complex-based luminescent probe.

Bioorg Chem 2021 Feb 19;110:104749. Epub 2021 Feb 19.

Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832002, China. Electronic address:

Real-time monitoring of drug metabolism in vivo is of great significance to drug development and toxicology research. The purpose of this study is to establish a rapid and visual in vivo detection method for the detection of an intermediate metabolite of the gold (I) drug. Gold (I) drugs such as sodium aurothiomalate (AuTM) have anti-inflammatory effects in the treatment of rheumatoid arthritis. Gold(III) ions (Au) are the intermediate metabolite of gold medicine, and they are also the leading factor of side effects in the treatment of patients. However, the rapid reduction of Au to Au by thiol proteins in organisms limits the in-depth study of metabolism of gold drugs in vivo. Here we describe a luminescence Au probe (RA) based on ruthenium (II) complex for detecting Au in vitro and in vivo. RA with large Stokes shift, good water solubility and biocompatibility was successfully applied to detect Au in living cells and vivo by luminescence imaging, and to trap the fluctuation of Au level produced by gold (I) medicine. More importantly, the luminescent probe was used to the detection of the intermediate metabolites of gold (I) drugs for the first time. Overall, this work offers a new detection tool/method for a deeper study of gold (I) drugs metabolite.
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http://dx.doi.org/10.1016/j.bioorg.2021.104749DOI Listing
February 2021

Differences in Performance of Immunosensors Constructed Based on CeO-Simulating Auxiliary Enzymes.

ACS Biomater Sci Eng 2021 03 22;7(3):1058-1064. Epub 2021 Feb 22.

School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, No. 221, Beisi Road, Xinjiang Uygur Autonomous Region, Shihezi 832000, China.

The morphology effect of cerium oxide (CeO) has always been the focus of catalysis research. Few people have reported the relationship between the morphology of CeO and electrochemical performance in sensors. In this paper, a polyaniline (PANI) matrix is used as the dispersant and stabilizer, ultrafine Au nanoparticles (NPs) (Au@PANI) are uniformly embedded in the PANI matrix, and Au NPs@PANI is fixed on the surface of CeO with different morphologies and sizes (Spindle CeO:(SCe), octahedron CeO (OCe)). The morphology and crystal structure of CeO were adjusted under different ratios of ethanol and water, and the effect of CeO was evaluated. The synthesized CeO-Au@PANI has different morphologies, sizes, and electrochemical properties. The electrochemical catalytic behavior of CeO-Au@PANI was studied by using hydrogen peroxide (HO) as the reaction substrate. The instantaneous current method (-) was used to further study the electrochemical amplification effect, and the best performance was obtained.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01680DOI Listing
March 2021

Ultrasensitive electrochemical immunosensor based on the signal amplification strategy of the competitive reaction of Zn and ATP ions to construct a "signal on" mode GOx-HRP enzyme cascade reaction.

Mikrochim Acta 2021 Jan 29;188(2):61. Epub 2021 Jan 29.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, People's Republic of China.

A GOx/HRP@ZIF-90 nanomaterial is proposed by coating GOx and HRP in ZIF-90 using a bio-simulated mineralization method to improve the tolerance of the enzyme to the external environment. In the detection process, the ZIF-90 is turned on under mild conditions by the competitive reaction of ATP with Zn and imidazole-2-carboxaldehyde (2-ICA), and the electrical signal of the system is amplified by the enzyme cascade reaction of GOx and HRP. Finally, based on the signal amplification strategy of the competitive reaction between Zn and ATP to construct a "signal on" mode, electrochemical immunosensor of GOx-HRP enzyme-linked cascade reaction was prepared. The proposed electrochemical immunosensor shows an excellent analytical performance when detecting CA-125, with good selectivity and stability, with a detection range of 0.1 pg mL-40 ng mL and a detection limit of 0.05 pg mL. The test has been performed using chronoamperometry under a constant voltage of -0.4 V. The immunosensor also shows an excellent performance when analysing human blood samples. The recovery of the immunosensor is 97.94-101.8%, with a relative standard deviation of 3.7-6.1%. The proposed sensor provides a novel idea for clinical use of GOx and HRP enzymes and a new method for the clinical detection of tumor markers.
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http://dx.doi.org/10.1007/s00604-021-04720-5DOI Listing
January 2021

A sandwich-type electrochemical immunosensor for ultrasensitive detection of multiple tumor markers using an electrical signal difference strategy.

Talanta 2020 Nov 5;219:121322. Epub 2020 Jul 5.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, PR China. Electronic address:

Traditional sandwich-type electrochemical immunosensors can only detect single tumor markers because signal interference occurs when detecting multiple tumor markers. In this work, an electrical signal difference strategy was proposed for the accurate detection of multiple tumor markers. We labeled PdAgCeO mesoporous nanospheres with a carcinoembryonic antigen (CEA) secondary antibody and MnO nanosheets labeled with an alpha-fetoprotein (AFP) secondary antibody. The two electrical signal tags were mixed and incubated on a prepared immunosensor to catalyze HO and generate an electrical signal I (i-t ampere curve). When 2.5 mM ascorbic acid solution (AA) was added to 20 mL of PBS solution at pH = 6.5 for 180 s, an electrical signal I was generated. I was the current response of the CEA antigen concentration, and the electrical signal difference ΔI = I-I was the current response of the AFP antigen. Thus, the immunosensor accurately detected the AFP and CEA tumor markers. This method was called the electrical signal difference strategy. The proposed single-use immunosensor detected CEA antigens in a range of 0.001 ng/mL-40 ng/mL, and the detection limit was 0.5 pg/mL; the detection range of the AFP antigen was 0.005 ng/mL-100 ng/mL, and the detection limit was 1 pg/mL. Therefore, this study provides new ideas and strategies for accurate clinical detection of multiple tumor markers.
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http://dx.doi.org/10.1016/j.talanta.2020.121322DOI Listing
November 2020

Bimetallic PtCu nanoparticles supported on molybdenum disulfide-functionalized graphitic carbon nitride for the detection of carcinoembryonic antigen.

Mikrochim Acta 2020 09 2;187(9):538. Epub 2020 Sep 2.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, China.

A molybdenum disulfide based graphite phase carbon nitride (MoS/g-CN) which is supported by a platinum-copper nanoparticle (PtCu) Z-type catalyst was created in this study. The catalyst exploits optoelectronic synergistic effect with large surface area, good catalysis, and biocompatibility to amplify the signal. The electrode impedance of the synthesized MoS/g-CN-PtCu was reduced five times in visible light compared with dark conditions, thereby improving the detection of carcinoembryonic antigen (CEA). At a voltage of - 0.4 V, the immunoprobe constructed with this material is used for CEA detection. A linear relationship between 100 fg mL and 80 ng mL concentrations was achieved with a minimum detection limit of 33 fg mL (S/N = 3). The recovery rate was 103-104%, and the relative standard deviation was 2.9-3.8%. This implies that the sandwich immunosensors have good reproducibility, selectivity, and stability and can be used in various applications. Graphical Abstract.
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http://dx.doi.org/10.1007/s00604-020-04498-yDOI Listing
September 2020

Sensitive detection of carcinoembryonic antigen (CEA) by a sandwich-type electrochemical immunosensor using MOF-Ce@HA/Ag-HRP-Ab as a nanoprobe.

Nanotechnology 2020 May 28;31(18):185605. Epub 2020 Jan 28.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, People's Republic of China.

Sandwich-type electrochemical immunosensor was one of the main methods for detecting carcinoembryonic antigen (CEA). In this work, using Ce-MoF as the skeleton precursor, hyaluronic acid (HA) was coated on the surface of Ce-metal organic framework (Ce-MoF), which loaded with silver nanoparticles (Ag NPs) and horseradish peroxidase (HRP) to catalyze HO and double amplified the current signal. Thus, a sensitive sandwich-type electrochemical immunosensor (Ce-MoF@ HA/Ag-HRP) was designed to detect carcinoembryonic antigen (CEA). The designed immunosensor used Au NPs to enhance the ability of attach more the first antibody (Ab). This was due to Au NPs had good electrical conductivity and biocompatibility to accelerate electron transfer on the surface of the electrode. HA was riched in -COOH, -OH and had excellent biocompatibility, which can carry more Ag NPs to catalyze HO. Finally, the prepared sandwich-type electrochemical immunosensor had excellent biocompatibility and great catalytic performance. The immunosensor can be tested within 30 min and the logarithm of the current signal and CEA concentration showed a broad linear response range of 1 pg ml-80 ng ml, and the detection limit of CEA was 0.2 pg ml. More importantly, the proposed immunosensor had good reproducibility, selectivity, stability and without matrix effect. This confirmed that the proposed immunosensor had broad prospects in early clinical trials.
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http://dx.doi.org/10.1088/1361-6528/ab70d3DOI Listing
May 2020

A sandwich-type electrochemical immunosensor for detecting CEA based on CeO-MoS absorbed Pb.

Anal Biochem 2020 03 3;592:113566. Epub 2020 Jan 3.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, PR China.

A sandwich-type immunosensor for detecting the concentration of carcinoembryonic antigen (CEA) was prepared. In this work, gold nanoparticles (Au NPs) were used as platform to attach more primary antibody (Ab) due to excellent electrical conductivity and good biocompatibility. Molybdenum disulfide-Cerium oxide (CeO-MoS) nanohybrid was used as a carrier to absorb lead ions (Pb) and the second antibody (Ab). CeO-MoS-Pb-Ab was used as a nanoprobe to detect CEA antigen. Under optimal conditions, square wave voltammetry (SWV) successfully displayed the electrical signal of Pb. The designed electrochemical immunosensor has excellent analytical performance. In addition, the detection range was 0.001-80 ng/mL and the minimum detection limit was 0.3 pg/mL (S/N = 3), which had good selectivity and stability. Finally, the proposed immunosensor successfully detected the concentration of CEA in the serum of the sample, which provided a feasible method for CEA testing.
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http://dx.doi.org/10.1016/j.ab.2019.113566DOI Listing
March 2020

Microwave-assisted preparation of ZnFeO-Ag/rGO nanocomposites for amplification signal detection of alpha-fetoprotein.

Bioelectrochemistry 2020 Apr 30;132:107434. Epub 2019 Nov 30.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China. Electronic address:

In this study, a novel signal-amplified immunosensor was designed by using a microwave-assisted self-assembly method to synthesize ZnFeO-Ag/rGO nanocomposites. The conductivity of ZnFeO-rGO nanocomposites was significantly improved due to the effective inhibition of rGO accumulation by the insertion of ZnFeO and Ag nanoparticles (NPs) into graphene sheets. Excellent sensitivity and reproducibility were achieved through the microwave-assisted preparation of ZnFeO-Ag/rGO nanocomposites as a substrate, with the Ag NPs enhancing the signal because of the effective conductive matrix. The layer assembly process of the immunosensor was verified by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the fabricated immunosensor showed good linearity over a wide concentration range from 1 pg mL to 200 ng mL with a low detection limit of 0.98 pg mL, and exhibited excellent specificity, good stability, and reproducibility. These qualities can contribute to the successful application of a label-free immunosensor in the detection of AFP in human serum.
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http://dx.doi.org/10.1016/j.bioelechem.2019.107434DOI Listing
April 2020

Sandwich-type electrochemical immunosensor constructed using three-dimensional lamellar stacked CoS@C hollow nanotubes prepared by template-free method to detect carcinoembryonic antigen.

Anal Chim Acta 2019 Dec 4;1088:54-62. Epub 2019 Sep 4.

College of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, PR China.

Effective treatment of cancer depends on early detection of tumor markers. In this paper, an effective template-free method was used to prepare CoS@C three-dimensional hollow sheet nanotubes as the matrix of the immunosensor. The unique three-dimensional hybrid hollow tubular nanostructure provides greater contact area and enhanced detection limit. The CoS@C-NH-HRP nanomaterial was synthesized as a marker and had a high specific surface area, which can effectively improve the electrocatalytic ability of hydrogen peroxide (HO) reduction while increasing the amount of capture-fixed carcinoembryonic antigen antibody (anti-CEA). In addition, the co-bonded horseradish peroxidase (HRP) can further promote the redox of HO and amplify the electrical signal. Carcinoembryonic antigen (CEA) was quantified by immediate current response (i-t), and the prepared immunosensor had good analytical performance under optimized conditions. The current signal and the concentration of CEA were linear in the range of 0.001-80 ng/mL, and the detection limit was 0.33 pg/mL (S/N = 3). The designed immunosensor has good selectivity, repeatability and stability, and the detection of human serum samples shows good performance. Furthermore, electrochemical immunosensor has broad application prospects in the clinical diagnosis of CEA.
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http://dx.doi.org/10.1016/j.aca.2019.09.007DOI Listing
December 2019

An electrochemical immunosensor for simultaneous point-of-care cancer markers based on the host-guest inclusion of β-cyclodextrin-graphene oxide.

J Mater Chem B 2016 Feb 11;4(5):990-996. Epub 2016 Jan 11.

School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.

A novel electrochemical immunosensor was developed for the simultaneous detection of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) using CuO-graphene oxide-β-cyclodextrin (CuO-GO-CD) and β-cyclodextrin-graphene oxide-ferrocenecarboxylic acid (GO-CD-Fc-COOH) as the distinguishable signal probe, and graphene oxide-gold nanoparticles (GO-AuNPs) as the sensor platform. GO-CD displayed excellent solubility in water and rich capture capability to Fc-COOH and the secondary antibody. The proposed immunosensor exhibited an excellent electrochemical performance. The linear ranges were from 0.001 ng mL to 80 ng mL for AFP and CEA with a detection limit of 0.0002 ng mL for AFP and 0.0001 ng mL for CEA. With the merits of acceptable stability, high sensitivity, a wide linear range and a low detection limit, the proposed immunosensor showed great potential for the simultaneous detection of multi-analytes in clinical diagnosis.
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http://dx.doi.org/10.1039/c5tb02480cDOI Listing
February 2016

A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe₃O₄@SiO₂ as labels.

Biosens Bioelectron 2016 May 11;79:48-54. Epub 2015 Nov 11.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China. Electronic address:

A sandwich-type electrochemical immunosensor was developed for sensitive detection of carcinoembryonic antigen (CEA) by using ferroferric oxide@silica-amino groups (Fe3O4@SiO2-NH2) as carriers and gold nanoparticles-graphene oxide (GO-AuNPs) as platform. The Fe3O4@SiO2-NH2 surface was used as linked reagents for co-immobilization of ferrocenecarboxylic acid (Fc-COOH) and secondary anti-CEA (Ab2) to prepare the signal probe, and it also could hasten the decomposition of hydrogen peroxide (H2O2) to amplify signals. Differential pulse voltammetry (DPV) was successfully used to quantify CEA. Under the optimized conditions, the designed immunosensor shows an excellent analytical performance wide dynamic response range of CEA concentration from 0.001 ng mL(-1) to 80 ng mL(-1) with a relatively low detection limit of 0.0002 ng mL(-1) (S/N=3), and high specificity and good reproducibility. The proposed immunosensor was successfully used to determine CEA in spiked human serum samples.
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http://dx.doi.org/10.1016/j.bios.2015.11.001DOI Listing
May 2016

Graphene oxide supported rhombic dodecahedral Cu2O nanocrystals for the detection of carcinoembryonic antigen.

Anal Biochem 2016 Feb 18;494:101-7. Epub 2015 Nov 18.

School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi 832003, People's Republic of China. Electronic address:

In this work, a simple electrochemical immunosensor was developed for the detection of carcinoembryonic antigen (CEA) based on rhombic dodecahedral Cu2O nanocrystals-graphene oxide-gold nanoparticles (rCu2O-GO-AuNPs). GO as the template and surfactant resulting in rCu2O exhibit improved rhombic dodecahedral structure uniformity and excellent electrochemical performance. Moreover, GO was found to be able to effectively improve the long stability of rCu2O on the electrode response. Under optimal conditions, the immunosensor showed a low limit of detection (0.004 ng ml(-1)) and a large linear range (0.01-120 ng ml(-1)). This work presents a potential alternative for the diagnostic applications of GO-supported special morphology materials in biomedicine and biosensors.
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http://dx.doi.org/10.1016/j.ab.2015.11.004DOI Listing
February 2016

Ultrathin gold nanowire-functionalized carbon nanotubes for hybrid molecular sensing.

ACS Nano 2013 Sep 3;7(9):7805-11. Epub 2013 Sep 3.

Department of Chemistry, University of Kansas , Lawrence, Kansas, 66045, United States.

Carbon nanotubes (CNTs) have shown great potential as sensing component in the electrochemical field effect transistor and optical sensors, because of their extraordinary one-dimensional electronic structure, thermal conductivity, and tunable and stable near-infrared emission. However, the insolubility of CNTs due to strong van der Waals interactions limits their use in the field of nanotechnology. In this study, we demonstrate that noncovalent ultrathin gold nanowires functionalized multiwalled carbon nanotube (GNW-CNT) hybrid sensing agents show highly efficient and selective immune molecular sensing in electrochemical and near-infrared photoacoustic imaging methods. A detection limit of 0.01 ng/mL for the alpha-fetoprotein (AFP) antigen with high selectivity is shown. The extraordinary optical absorption, thermal, and electric conductivity of hybrid GNW-CNTs presented in this study could be an effective tactic to integrate imaging, sensing, and treatment functionalities.
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http://dx.doi.org/10.1021/nn4027323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946550PMC
September 2013

Preparation of a composite film electrochemically deposited with chitosan and gold nanoparticles for the determination of alpha-1-fetoprotein.

Bioprocess Biosyst Eng 2010 Jun 27;33(5):613-8. Epub 2009 Oct 27.

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, People's Republic of China.

A novel amperometric immunosensor based on chitosan-gold nanoparticles (Chit-GNPs) composite film and thionine (Thi) was prepared for the determination of alpha-1-fetoprotein (AFP). The immunosensor was prepared by electro-depositing a Chit-GNPs composite matrix on the surface of the glass carbon electrode, then Thi was immobilized onto the Chit-GNPs film using glutaraldehyde as a cross-linker. Furthermore, the GNPs were chemisorbed onto Thi film for immobilization of alpha-1-fetoprotein antibody. The procedure of the immunosensor was characterized by means of cyclic voltammograms. The performance and influencing factors of the resulting immunosensor were studied in details. Under optimal conditions, the immunosensor was highly sensitive to AFP and the linear range covered from 0.40 to 200.0 ng mL(-1) with a detection limit of 0.24 ng mL(-1) at three times background noise. Moreover, the simple and controllable electro-deposition method overcame the irreproducibility for preparing Chit-based immunosensor systems and the proposed immunosensor displayed a satisfactory reproducibility and stability.
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http://dx.doi.org/10.1007/s00449-009-0385-yDOI Listing
June 2010

Porous redox-active Cu2O-SiO2 nanostructured film: preparation, characterization and application for a label-free amperometric ferritin immunosensor.

Talanta 2009 Apr 13;78(2):596-601. Epub 2008 Dec 13.

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

A novel Cu(2)O-SiO(2) nanostructured particle was synthesized by a solution-phase method and was adopted for construction of a label-free amperometric immunosensor. The porous Cu(2)O-SiO(2) nanoparticles had good redox electrochemical activity, large surface-to-volume ratio, film-forming ability and high stability. The physical morphology and structure of Cu(2)O-SiO(2) nanoparticles were examined by scanning electron microscope (SEM) and transmission electron microscopy (TEM). The chemical component of Cu(2)O-SiO(2) was confirmed by X-ray photoelectron spectroscopy (XPS) and auger electron spectra (AES). The electrode modification process was probed by cyclic voltammetry (CV) and the performance of the immunosensor was studied by differential pulse voltammetry (DPV) measurements. To improve the analytical characteristics of the immunosensor, the experimental conditions were optimized. The immunosensor exhibited a good response to ferritin in ranges from 1.0 to 5.0 and 5.0 to 120.0 ng mL(-1) with a detection limit of 0.4 ng mL(-1). The fabricated immunosensor could make a low-cost, sensitive, quantitative detection of ferritin, and would have a potential application in clinical immunoassays.
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http://dx.doi.org/10.1016/j.talanta.2008.12.017DOI Listing
April 2009

Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3.

Anal Chim Acta 2009 Feb 6;633(2):244-9. Epub 2008 Dec 6.

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of Fc-COOH and were easily modified with trialkoxysilane reagents for covalent conjugation of CA 15-3 antibodies (anti-CA 15-3). The Fc-COOH-doped SNPs were characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was demonstrated by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the developed immunosensor showed good linearity at the studied concentration range of 2.0-240 UmL(-1) with a coefficient 0.9986 and a detection limit of 0.64 UmL(-1) at S/N=3.
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http://dx.doi.org/10.1016/j.aca.2008.11.068DOI Listing
February 2009