Publications by authors named "Chengjie Xie"

12 Publications

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Evaluation of the relationship among dental fear, scaling and root planing and periodontal status using periodontitis stages: A retrospective study.

J Dent Sci 2022 Jan 21;17(1):293-299. Epub 2021 Apr 21.

Department of Periodontology, Stomatological Hospital, Southern Medical University, Guangzhou, China.

Background/purpose: Patients with periodontal disease have higher dental fear levels, which may have negative effects on their clinical outcome during scaling and root planing (SRP). The present study used the new classification of periodontitis and validated questionnaires to assess the relationship among dental fear, SRP pain and periodontal status.

Materials And Methods: A total of 120 periodontitis patients were enrolled and staging according to the new classification of periodontitis. SRP was performed, and the visual analog scale (VAS) to assess pain was used with every patient after treatment. Questionnaires, including Corah's Dental Anxiety Scale (DAS), Dental Fear Survey (DFS), and short-form Dental Anxiety Inventory (S-DAI) were implemented from the first attendance and subsequent visits after 6 months. The patients were grouped by DAS scores. The statistical analysis was performed using T-test, chi-square, Pearson and Spearman correlative analysis.

Results: Compared to pre-SRP treatment, the dental fear level on DFS was decreased in the posttreatment period for all periodontitis stages. There were no statistically significant differences in S-DAI and DAS between pretreatment and posttreatment periods in stage I and II; meanwhile, there were statistically differences in stage III and IV. The correlation among periodontitis stages, VAS and dental fear level was significant. The proportion of high periodontitis stages was increased in high dental fear group.

Conclusion: SRP can reduce dental fear levels in all periodontitis stages, especially in stage III and IV. Correlations exist among periodontal status, dental fear and SRP pain. High dental fear is associated with poor periodontal status.
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http://dx.doi.org/10.1016/j.jds.2021.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739248PMC
January 2022

Evaluation of factors affecting alveolar ridge height and facial bone thickness in Chinese maxillary central incisors by cone beam CT.

J Dent Sci 2021 Jan 8;16(1):229-235. Epub 2020 Jun 8.

Department of Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, 510280, China.

Background/purpose: In the immediate implantation of maxillary central incisors, the height of the alveolar bone is lost, and there is often a risk of bone fracture due to the thin buccal bone wall (BBW). The purpose of this study was to assess the effects of smoking, age, and root position in the alveolar bone on the BBW and the distance between the cemento-enamel junction (CEJ) and the facial bone crest (FBC) of Chinese maxillary central incisors.

Materials And Methods: The patients were divided by smoking, gender, age, and root sagittal position in the alveolar bone. BBW thickness was measured at the following sites: the 4 mm apical to the CEJ, the middle of the root, and the apex. The distance from the CEJ to the FBC was also evaluated.

Results: Cone beam CT (CBCT) data for the maxillary central incisors of 645 patients (323 males and 322 females) were selected and analyzed. The CEJ-FBC distance in patients who smoked (2.79 ± 0.78 mm) was significantly greater than that of non-smokers (2.54 ± 0.69 mm). The BBW in subtype III (0.74 ± 0.43 mm, 0.81 ± 0.36 mm) was thinner than that in subtypes I and II at 4 mm apical to the CEJ and in the middle of the root, with a statistically significant difference ( < 0.05).

Conclusion: In most Chinese people, smoking, gender, age, and the position of the root in alveolar bone are all important factors that must be considered before immediate implantation is undertaken.
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http://dx.doi.org/10.1016/j.jds.2020.05.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770285PMC
January 2021

Efficient tidal channel networks alleviate the drought-induced die-off of salt marshes: Implications for coastal restoration and management.

Sci Total Environ 2020 Dec 4;749:141493. Epub 2020 Aug 4.

School of Environment, State Key Laboratory of Water Environment Simulation, Beijing Normal University, Beijing 100875, China; Yellow River Estuary Wetland Ecosystem Observation and Research Station, Ministry of Education, Shandong 257500, China. Electronic address:

Massive die-off in salt marshes is one of the most common examples of widespread degradation in marine and coastal ecosystems. In salt marshes, tidal channel networks facilitate the exchange of water, nutrients, sediments and biota with the open marine environments. However, quantitative analyses of the role of channel networks in alleviating vegetation die-off in salt marshes are scarce. Here we quantified the spatial-temporal development of marsh vegetation die-off in the northern Liaodong Bay by analyzing aerial images before, during, and after a drought (from 2014 to 2018). We found that Suaeda salsa marshes have recently experienced large-scale die-off. The extent of vegetation die-off increases with increasing distance from the channel network. Moreover, our results suggested that efficient tidal channel networks (high drainage density, low mean unchanneled path length) can mitigate die-off at the watershed scale. We presented possible abiotic & biotic processes in channel networks that explain this spatial dynamic. Our study highlights the importance of efficient tidal channel networks in mitigating die-off and enhancing the resistance of marshes to droughts, and call for incorporating theses dynamics in coastal restoration and management.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141493DOI Listing
December 2020

Consumer control and abiotic stresses constrain coastal saltmarsh restoration.

J Environ Manage 2020 Nov 8;274:111110. Epub 2020 Aug 8.

State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China; Yellow River Estuary Wetland Ecosystem Observation and Research Station, Ministry of Education, Shandong, 257500, China. Electronic address:

Die-off of coastal wetlands has been reported worldwide. Planting habitat-forming species is an important strategy to reverse the decline of coastal wetlands. However, how abiotic environmental stresses and consumers affect the establishment of the planted vegetation species is unclear. We reported a large-scale restoration project in the Liaohe estuary, China, where native pioneer plant Suaeda salsa was planted. We evaluated the growth performance of the planted S. salsa, and identified the constraints on the establishment of planted S. salsa. Results showed that the growth performance (density, coverage and survival rate) of planted S. salsa was better in the low restored marsh than that in the high restored marsh. The death of planted S. salsa was primarily driven by crab herbivory, followed by abiotic stresses (low soil moisture and high salinity) in the high restored marsh, whereas plant death was only driven by crab herbivory in the low restored marsh. Herbivory strength in the high marsh was significantly higher than that in the low marsh. Our findings challenge the bottom-up paradigm used as the foundation for coastal restoration, and highlight the overlooked role of consumers. Therefore, protection measures against consumer pressure, especially in physically harsh conditions, should be considered to enhance the success of coastal wetland restoration.
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http://dx.doi.org/10.1016/j.jenvman.2020.111110DOI Listing
November 2020

Reclamation shifts the evolutionary paradigms of tidal channel networks in the Yellow River Delta, China.

Sci Total Environ 2020 Nov 29;742:140585. Epub 2020 Jun 29.

State Key Laboratory of Water Environment Simulation & School of Environment, Beijing Normal University, Beijing 100875, China; Yellow River Estuary Wetland Ecosystem Observation and Research Station, Ministry of Education, Shandong 257500, China.

Tidal channel networks are ubiquitous features of coastal landforms that control the input and output of intertidal water, sediment and nutrients. Nevertheless, those intertidal platforms have undergone extensive losses due to human activities such as land reclamation. Identifying how tidal channel networks respond to land reclamation is critical to our prediction of the fate of residual tidal landforms. However, the morphological changes in the channel networks in the Yellow River Delta (YRD) impacted by severe reclamation remain unclear. Here, we analyzed the spatiotemporal dynamics of channel networks on two scales (the delta scale and the zone scale) under the double stress of land reclamation and natural pressure by comparing a comprehensive suite of morphological channel characteristics, including channel segment count, channel order, length, fractal dimension, drainage density and drainage efficiency. The results show that the interannual dynamics of tidal channel networks in the delta over the last three decades have experienced two periods: a favorable period during 1984-2000 and an adverse period during 2001-2018. The spatiotemporal patterns of channel networks varied with zones. Land reclamation exerted a dominant influence on shifting the evolutionary trend of channel networks on both the delta scale and the zone scale when reclamation proportion exceeded a certain threshold. Sediment siltation could to a certain degree mitigate the impact of reclamation on tidal channel networks development. Our study highlighted the effect of reclamation on the geomorphological evolution of tidal channel networks and identified its impact threshold which could further be used to guide coastal zone restoration and management.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140585DOI Listing
November 2020

Hydrological connectivity dynamics of tidal flat systems impacted by severe reclamation in the Yellow River Delta.

Sci Total Environ 2020 Oct 2;739:139860. Epub 2020 Jun 2.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China; Yellow River Estuary Wetland Ecosystem Observation and Research Station, Ministry of Education, Shandong, 257500, China.

River deltas contain complex self-organizing channel networks that continuously exchange fluxes of water, matter, energy, and information with their surroundings. The connectivity of these exchange processes plays a crucial role in controlling the evolution and dynamic stability of river deltas. However, connectivity patterns related to tidal channel networks have rarely been studied, especially in the Yellow River Delta (YRD), which is impacted by severe reclamation. Here, we evaluated the potential hydrological connectivity dynamics between the tidal channel network and its surroundings using an index of connectivity (IC) in the whole YRD and its three sub-regions: erosion zone, oil field zone and deposition zone. The results suggested that different areas had different spatial connectivity potential. The mean value of the IC related to the channel networks showed little difference for any zones. However, the total connectivity response area (CRA; set of connectivity response units) varied with the study scale. A decreasing trend was found on the delta scale and a relatively stable trend was found in the deposition zone. In terms of dynamic connectivity, the tidal flat system did not show a continuous trend over time. Our results indicated that the YRD is such a dynamic complex that a relatively stable connectivity pattern is unlikely to be achieved over time. Therefore, future ecological restoration based on hydrological connectivity needs to consider more related influencing factors and their temporal and spatial dynamics.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139860DOI Listing
October 2020

Generation and characterization of a monoclonal antibody against human BCL6 for immunohistochemical diagnosis.

PLoS One 2019 7;14(5):e0216470. Epub 2019 May 7.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

Background: Human B-cell lymphoma 6 (BCL6) gene, usually coding protein of 706 amino acids, is closely associated with large B cell lymphoma. Researches showed that protein mutation or change of expression levels usually happened in the mounting non-hodgkin lymphoma (NHL). Thus BCL6 is considered to be involved in germinal center (GC)-derived lymphoma.

Results: The BCL61-350 gene codons were optimized for prokaryotic system. After expression of BCL61-350 in E. coli, the BCL61-350 protein was purified with Ni column. Then the BCL61-350 protein, mixing with QuickAntibody-Mouse5W adjuvant, was injected into Balb/c mice. After immunization and cell fusion, a stable cell line named 1E6A4, which can secrete anti-BCL6 antibody, was obtained. The isotype of 1E6A4 mAb was determined as IgG2a, and the affinity constant reached 5.12×1010 L/mol. Furthermore, the specificity of the mAb was determined with ELISA, western blot and immunohistochemistry. Results indicated that the 1E6A4 mAb was able to detect BCL6 specifically and sensitively.

Conclusions: BCL61-350 antigen has been successfully generated with an effective and feasible method, and a highly specific antibody named 1E6A4 against BCL6 has been screened and characterized in this study, which was valuable in clinical diagnosis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0216470PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504089PMC
January 2020

Preparation of Anti-Human Podoplanin Monoclonal Antibody and its application in Immunohistochemical Diagnosis.

Sci Rep 2018 07 5;8(1):10162. Epub 2018 Jul 5.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Podoplanin (PDPN), a 38 kDa transmembrane sialoglycoprotein from human, is expressed in lymphatic endothelial cells but not in vascular endothelial cells, and has been considered as a specific marker of lymph. In this study, the gene encoding the extracellular part of PDPN (ePDPN) was synthesized and used to expressed fusion protein ePDPN-His and GST-ePDPN, respectively, in E.coli. The purified GST-ePDPN fusion protein was mixed with QuickAntibody-Mouse5W adjuvant to immune mice, and the antiserum titer was determined by indirect ELISA. A stable cell line named 5B3 generating anti-PDPN monoclonal antibody (mAb) was obtained by hybridoma technology. The isotype of 5B3 cell line was IgG, and the chromosome number was 102 ± 4. The 5B3 mAb was purified successfully from ascites fluid through Protein G column, and its affinity constant was 2.94 × 10 L/mol. Besides, excellent specificity of the 5B3 mAb was further demonstrated in ELISA, western blot and immunohistochemistry experiments, suggesting that 5B3 mAb displays similar application value to D2-40, a commercial available antibody. Hence, the current study provides conclusive guidelines for preparation of other mAbs and their applications in immunohistochemistry diagnosis.
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http://dx.doi.org/10.1038/s41598-018-28549-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033854PMC
July 2018

Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis.

BMC Biotechnol 2018 05 4;18(1):25. Epub 2018 May 4.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker.

Results: The synthesized gene of CgA coding area was cloned and expressed as fusion protein CgA-His in procaryotic system. Then the purified CgA-His protein was mixed with QuickAntibody-Mouse5W adjuvant, and injected into mice. The CgA-His protein was also used as coating antigen to determine the antiserum titer. By screening, a stable cell line named 4E5, which can generate anti-CgA monoclonal antibody (mAb), was obtained. The isotype of 4E5 mAb was IgG, and the chromosome number was 102 ± 4. Anti-CgA mAb was purified from ascites fluid, and the affinity constant reached 9.23 × 10 L/mol. Furthermore, the specificity of the mAb was determined with ELISA, western blot and immunohistochemistry. Results indicated that the mAb 4E5 was able to detect chromogranin A specifically and sensitively.

Conclusions: A sensitive and reliable method was successfully developed for rapid production of anti-CgA mAb for immunohistochemistry diagnosis in this study, and the current study also provides conclusive guidelines for preparation of mAbs and implements in immunohistochemistry diagnosis.
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http://dx.doi.org/10.1186/s12896-018-0436-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935939PMC
May 2018

Preparation of Monoclonal Antibody for Brevetoxin 1 and Development of Ic-ELISA and Colloidal Gold Strip to Detect Brevetoxin 1.

Toxins (Basel) 2018 02 8;10(2). Epub 2018 Feb 8.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Brevetoxin-1 (BTX-1), a marine toxin mostly produced by the dinoflagellatae , has caused the death of marine organisms and has had numerous toxicological effects on human health. Hence, it is very necessary to develop a rapid, economical, and reliable immunoassay method for BTX-1 detection. In this study, two kinds of complete antigen were synthesized using the succinic anhydride and isobutyl chloroformate two-step methods. Conjugate BTX-1-OVA was used as an antigen for mice immunization, and BTX-1-BSA for measuring the titer of the produced antibodies. A hybridoma cell line 6C6 stably secreting monoclonal antibody (mAb) against BTX-1 was obtained by fusing SP2/0 myeloma cells with the spleen cells from the immunized mouse. The hybridoma 6C6 was injected into the abdomen of BALB/c mice to obtain ascites, and the anti-BTX-1 mAb was harvested from ascites by precipitation with caprylic acid/ammonium sulfate (CA-AS). The anti-BTX-1 mAb was identified as an IgG1 subtype, and the cross-reactivity results showed that anti-BTX-1 mAb was highly specific to BTX-1 with the affinity of 1.06 × 10⁸ L/mol. The indirect competitive ELISA results indicated that the linear range for BTX-1 detection was 14-263 ng/mL with IC of 60 ng/mL, and a detection limit of 14 ng/mL. The average recovery rate from the spiked samples was 88 ± 2% in intra-assay and 89 ± 2% in inter-assay. The limit of detection (LOD) using the colloidal gold strip was 200 ng/mL with high specificity. Therefore, the anti-BTX-1 mAb can be used to detect BTX-1 in shellfish and other related samples.
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http://dx.doi.org/10.3390/toxins10020075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848176PMC
February 2018

Association between vitamin D receptor gene polymorphisms and severe chronic periodontitis in a Chinese population.

J Periodontol 2009 Apr;80(4):603-8

Department of Periodontology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China.

Background: Chronic periodontitis (CP) exhibits inflammation and alveolar bone loss, and severe forms of periodontitis are suggested to have a genetic basis. Vitamin D receptor (VDR) regulates bone metabolism and inflammation-related genes, and single nucleotide polymorphisms (SNPs) in the VDR gene may affect the functional activity of the VDR protein in CP. Therefore, the aim of this study was to investigate the association between VDR SNPs and severe CP in a Chinese population.

Methods: DNA was obtained from 107 patients with severe CP and 121 control subjects. The BsmI, TaqI, ApaI, and FokI SNPs of VDR genes were investigated by restriction fragment length polymorphism of polymerase chain reaction (PCR) products. The digested PCR products were electrophoresed on an 8% polyacrylamide gel and developed by the DNA silver staining method.

Results: The distribution of VDR TaqI genotypes and alleles between the two groups was significantly different (P = 0.019 and P = 0.039, respectively). The TT genotype and T allele seemed to increase the susceptibility of severe CP (odds ratio, 2.75; 95% confidence interval [CI]: 1.17 to 6.47; odds ratio, 2.28, 95% CI: 1.02 to 5.06, respectively) in Chinese populations. There was no significant difference in the genotype distribution or the allele frequencies of VDR BsmI, ApaI, or FokI between the two groups.

Conclusion: TaqI SNP of VDR gene might be associated with severe CP in Chinese patients.
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http://dx.doi.org/10.1902/jop.2009.080465DOI Listing
April 2009
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