Publications by authors named "Chenghao Wu"

11 Publications

  • Page 1 of 1

PHB blocks endoplasmic reticulum stress and apoptosis induced by MPTP/MPP in PD models.

J Chem Neuroanat 2021 04 11;113:101922. Epub 2021 Feb 11.

Heze Medical College, Heze 274000, China.

Ample empirical evidence suggests that mitochondrial dysfunction and endoplasmic reticulum (ER) stress play a crucial role in the pathogenesis of Parkinson's disease (PD). Prohibitin (PHB), a mitochondrial inner-membrane protein involved in mitochondrial homeostasis and function, may be involved in the pathogenesis of PD. We investigated the functional role of PHB in mitochondrial biogenesis and ER stress in methyl-4-phenylpyridinium (MPP +)-induced in vivo and in vitro models of PD. The overexpression of PHB in SH-SY5Y cells block ed cell death and the apoptosis induced by MPP + incubation. PHB also block ed the activation of ER stress markers, including glucose-regulated protein 78, while increasing the expression of Xbox- binding protein 1 and caspase-12. Moreover, the intracerebroventricular administration of the PHB overexpression vector greatly block ed motor dysfunction and the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurodegeneration in the mouse model of PD. The production of reactive oxygen species, ER stress, and autophagic stress induced by MPTP were also significantly block ed in PD mice overexpressing PHB. Our results suggest that PHB blocks the dopaminergic-neuron depletion by preserving mitochondrial function and inhibiting ER stress. The genetic manipulation of PHB may feature potential as a treatment for PD.
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http://dx.doi.org/10.1016/j.jchemneu.2021.101922DOI Listing
April 2021

Deletion of MicroRNA-144/451 Cluster Aggravated Brain Injury in Intracerebral Hemorrhage Mice by Targeting 14-3-3ζ.

Front Neurol 2020 12;11:551411. Epub 2021 Jan 12.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

This study aims at evaluating the importance and its underlying mechanism of the cluster of microRNA-144/451 (miR-144/451) in the models with intracerebral hemorrhage (ICH). A model of collagenase-induced mice with ICH and a model of mice with simple miR-144/451 gene knockout (KO) were used in this study. Neurodeficits and the water content of the brain of the mice in each group were detected 3 days after collagenase injection. The secretion of proinflammatory cytokines, such as tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β), as well as certain biomarkers of oxidative stress, was determined in this study. The results revealed that the expression of miR-451 significantly decreased in the mice with ICH, whereas miR-144 showed no significant changes. KO of the cluster of miR-144/451 exacerbated the neurological deficits and brain edema in the mice with ICH. Further analyses demonstrated that the KO of the cluster of miR-144/451 significantly promoted the secretion of TNF-α and IL-1β and the oxidative stress in the perihematomal region of the mice with ICH. In addition, the miR-144/451's depletion inhibited the regulatory axis' activities of miR-451-14-3-3ζ-FoxO3 in the mice with ICH. In conclusion, these data demonstrated that miR-144/451 might protect the mice with ICH against neuroinflammation and oxidative stress by targeting the pathway of miR-451-14-3-3ζ-FoxO3.
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http://dx.doi.org/10.3389/fneur.2020.551411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835478PMC
January 2021

Chronic stress induced depressive-like behaviors in a classical murine model of Parkinson's disease.

Behav Brain Res 2021 02 9;399:112816. Epub 2020 Aug 9.

Research Center for Sectional and Imaging Anatomy, Shandong University School of Medicine, Jinan, 250012, China. Electronic address:

Depression occurs in around 40 % of patients with Parkinson's disease (PD) and contributes to severe disability and a poor quality of life. The underlying mechanisms and pathophysiology of depression in PD (PDD) remain obscure, due to a lack of stable animal models of PDD. In this study, we established a PDD model by inducing exposure to chronic mild (CMS) and strong stress (CSS) using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in PD mice. We detected changes in motor and non-motor symptoms, brain structure, neurotransmitters, levels of 5-HT related genes and inflammation. CMS exposed PD (PDMS) mice exhibited obviously decreased levels of neuromuscular strength and enhanced levels of inflammation, compared with that of control mice. CSS exposed MPTP (PDSS) mice exhibited the highest level of motor impairment and depression states along with the highest levels of inflammation enhancement and a decrease in the expression levels of 5-hydroxytryptamine (5-HT) related genes in all groups. Our results suggested that CSS can successfully induce stable depression like symptoms in sub-chronic MPTP PD mice and appears to be a valuable tool for investigating PDD. Furthermore, it was found that 5-HT system dysfunction may contribute to depression like symptoms in PD.
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http://dx.doi.org/10.1016/j.bbr.2020.112816DOI Listing
February 2021

Total and Methylmercury of Suaeda heteroptera Wetland Soil Response to a Salinity Gradient Under Wetting and Drying Conditions.

Bull Environ Contam Toxicol 2020 Jun 19;104(6):778-785. Epub 2020 May 19.

Environmental College, Shenyang University, Shenyang, 110044, China.

Mercury (Hg) methylation could occur in freshwater ecosystems with low or high salinity. However, few studies are available about the effects of salinity change on mercury(Hg) release and methylation. In-situ experiments using Suaeda heteroptera wetland soil column from the Liaohe estuary were performed to decipher how total mercury (THg) and methylmercury (MeHg) contents change under fluctuant salinity and wet and dry soil conditions. Salinity gradients were set to 0.50% (S1), 1.00% (S2), 1.50% (S3) and 1.80% (S4), and pure deionized water was used as a blank control (CK). Wet and dry soil conditions were set to full inundation condition (WD1) and naturally dried treatment (WD2). Results indicated that the highest THg and MeHg contents were found in surface and bottom soil when water salinity treatment was CK under WD1. THg and MeHg decreased with salinity under WD1. THg contents in overlying water varied from 0.854 to 1.243 µg L under WD1 treatments and increased with salinity change. When under WD2 treatment, THg contents in both soil layers gradually decreased with rising salinity. Meanwhile, MeHg contents in both soil layers reached the lowest level at CK (1.666 μg kgand 2.520 μg kg) and increased gradually with the rising salinity. By comparison, THg content of the soil was much lower in WD1 than that in WD2. Under the WD1 condition, the MeHg contents and %MeHg decreased with rising salinity and showed significantly different in different salinity treatment, however, its showed an opposite trend with rising salinity under the WD2 condition.
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http://dx.doi.org/10.1007/s00128-020-02874-1DOI Listing
June 2020

Integrated Design of Hierarchical [email protected]@[email protected] Nanobox as Anode Material for Enhanced Lithium Storage Performance.

ACS Appl Mater Interfaces 2020 Apr 17;12(17):19768-19777. Epub 2020 Apr 17.

Shanghai Applied Radiation Institute, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.

Transition-metal oxides (TMOs) are potential candidates for anode materials of lithium-ion batteries (LIBs) due to their high theoretical capacity (∼1000 mA h/g) and enhanced safety from suppressing the formation of lithium dendrites. However, the poor electron conductivity and the large volume expansion during lithiation/delithiation processes are still the main hurdles for the practical usage of TMOs as anode materials. In this work, the [email protected]@[email protected] hierarchical nanobox (CNMN) is then proposed and fabricated to solve those issues. The as-prepared nanobox contains hollow cubic CoSnO as a core and dual N-doped carbon-"sandwiched" MnO particles as a shell. As anode materials of LIBs, the hollow and carbon interlayer structures effectively accommodate the volume expansion while dual active TMOs of CoSnO and MnO efficiently increase the specific capacity. Notably, the dual-layer structure of N-doped carbons plays a critical functional role in the incorporated composites, where the inner layer serves as a reaction substrate and a spatial barrier and the outer layer offers electron conductivity, enabling more effective involvement of active anode materials in lithium storage, as well as maintaining their high activity during lithium cycling. Subsequently, the as-prepared CNMN exhibits a high specific capacity of 1195 mA h/g after the 200th cycle at 0.1C and an excellent stable reversible capacity of about 876 mA h/g after the 300th cycle at 0.5C with only 0.07 mA h/g fade per cycle after 300 cycles. Even after a 250 times fast charging/discharging cycle both at 5C, it still retains a reversible capacity of 422.6 mA h/g. We ascribe the enhanced lithium storage performances to the novel hierarchical architectures achieved from the rational design.
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http://dx.doi.org/10.1021/acsami.9b22368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304665PMC
April 2020

Salinity and redox conditions affect the methyl mercury formation in sediment of Suaeda heteroptera wetlands of Liaoning province, Northeast China.

Mar Pollut Bull 2019 May 10;142:537-543. Epub 2019 Apr 10.

Environmental College, Shenyang University, Shenyang 110044, China; Key Laboratory of Eco-restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, China.

Using a laboratory simulation experiment, we studied the trend of change in methylmercury (MeHg) content of sediments in response to the changing salinity of flooding water (deionized water, 0.5%, 1.0%, 1.5%, and 2.0%) and sulfate-reducing bacteria (SRB) content for both the surface layer (0-10 cm) and the bottom layer (10-20 cm) of Suaeda heteroptera wetland sediments in the Liaohe estuary under anaerobic and aerobic conditions, respectively. The results showed that under AAC (anaerobic conditions), the MeHg content in the surface and bottom sediment layers increased first and then decreased over time and was highest at the 14th day. In contrast, under AC (aerobic conditions), the MeHg content in sediments of both layers increased slowly with increasing test time. The MeHg content in sediments increased first and then decreased with rising salinity and was highest at a salinity of 1.0%. Among the samples collected at different experimental stages, the SRB content in the sediments showed a decreasing trend with rising flooding salinity under AAC and AC. The MeHg and SRB contents were higher under anaerobic conditions than under aerobic conditions. Linear fitting results showed that there was no linear correlation between MeHg contents and SRB quantities in surface and bottom sediments under AAC and AC (R < 0.1). Collectively, these results suggest an important role for flooding salinity and anaerobic-aerobic conditions in the production of MeHg in S. heteroptera wetlands of the Liaohe estuary, and may predict the ecological risk of methylmercury according to the change of salinity.
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http://dx.doi.org/10.1016/j.marpolbul.2019.03.066DOI Listing
May 2019

Construction of SnS-SnO heterojunctions decorated on graphene nanosheets with enhanced visible-light photocatalytic performance.

Acta Crystallogr C Struct Chem 2019 Jun 29;75(Pt 6):812-821. Epub 2019 May 29.

School of Environmental and Chemical Engineering, Shanghai University, ShangDa Road 99, Shanghai 200444, People's Republic of China.

Heterostructures formed by the growth of one kind of nanomaterial in/on another have attracted increasing attention due to their microstructural characteristics and potential applications. In this work, SnS-SnO heterostructures were successfully prepared by a facile hydrothermal method. Due to the enhanced visible-light absorption and efficient separation of photo-generated holes and electrons, the SnS-SnO heterostructures display excellent photocatalytic performance for the degradation of rhodamine (RhB) under visible-light irradiation. Additionally, it is found that the introduction of graphene into the heterostructures further improved photocatalytic activity and stability. In particular, the optimized SnS-SnO/graphene photocatalyst can degrade 97.1% of RhB within 60 min, which is about 1.38 times greater than that of SnS-SnO heterostructures. This enhanced photocatalytic activity could be attributed to the high surface area and the excellent electron accepting and transporting properties of graphene, which served as an acceptor of the generated electrons to suppress charge recombination. These results provide a new insight for the design and development of hybrid photocatalysts.
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http://dx.doi.org/10.1107/S2053229619006399DOI Listing
June 2019

Molecular characterization of water-soluble organic compounds in PM2.5 using ultrahigh resolution mass spectrometry.

Sci Total Environ 2019 Jun 4;668:917-924. Epub 2019 Mar 4.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Water-soluble organic compounds (WSOCs) are a complex mixture of organic components with a variety of chemicals structures that may have significant impacts on the formation process and health hazards of atmospheric fine particles. In this study, the molecular characteristics of WSOCs in PM were investigated using ultrahigh resolution mass spectrometry. In total 7389 compounds in PM water extracts were identified, including CHO, CHOS, CHON, CHONS, CH, CHS, CHN and CHNS species. CHO and CHON were the major components in PM water extracts. S-containing compounds detected in both ionization modes were observed with distinct molecular characteristics. Selective partitioning of WSOCs between PM water extracts and polydimethylsiloxane (PDMS, log D = 0.51-3.87) coating phases was found, which was significantly correlated with molecular characteristic (i.e. double-bond equivalent, number of O and H atoms, O/C ratios, and aromaticity equivalent). The extent of accumulation for negatively charged compounds was generally lower, which related to the existence of polar functional groups, such as hydroxyl, carboxyl, nitrate, and sulfate, as observed by MS/MS fragmentation analysis.
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http://dx.doi.org/10.1016/j.scitotenv.2019.03.031DOI Listing
June 2019

Low Defect FeFe(CN)6 Framework as Stable Host Material for High Performance Li-Ion Batteries.

ACS Appl Mater Interfaces 2016 Sep 1;8(36):23706-12. Epub 2016 Sep 1.

College of Chemistry and Molecular Sciences, Hubei Key Laboratory of Electrochemical Power Sources, Wuhan University , Wuhan, Hubei 430072, China.

Low cost and high performance Li-ion batteries have been extensively pursued for grid-scale energy storage applications; however, their development has been impeded for a long time due to the lack of qualified cathode materials with not only decent electrochemical performance but also resource abundance and low price. In this paper, we report Prussian-blue type FeFe(CN)6 nanocrystals with well-controlled lattice defects and perfect nanocubic morphology, which can exhibit a high Li-storage capacity of 160 mAh g(-1), a strong rate performance at 24 C, and a superior cycle stability with 90% capacity retention over 300 cycles. This low defect lattice and its excellent Li-insertion performance might provide a new insight into the design of advanced Li-ion battery materials and also a competitive alternative to the presently developed Li(+) insertion cathodes to develop low cost and high performance Li-ion batteries for grid-scale energy storage applications.
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http://dx.doi.org/10.1021/acsami.6b06880DOI Listing
September 2016

Highly Crystallized Na₂CoFe(CN)₆ with Suppressed Lattice Defects as Superior Cathode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2016 Mar 17;8(8):5393-9. Epub 2016 Feb 17.

College of Chemistry and Molecular Science, Wuhan University , Wuhan 430072, China.

Prussian blue and its analogues have received particular attention as superior cathodes for Na-ion batteries due to their potential 2-Na storage capacity (∼170 mAh g(-1)) and low cost. However, most of the Prussian blue compounds obtained from the conventional synthetic routes contain large amounts of Fe(CN)6 vacancies and coordinated water molecules, which leads to the collapse of cyano-bridged framework and serious deterioration of their Na-storage ability. Herein, we propose a facile citrate-assisted controlled crystallization method to obtain low-defect Prussian blue lattice with greatly improved Na-storage capacity and cycling stability. As an example, the as-prepared Na2CoFe(CN)6 nanocrystals demonstrate a reversible 2-Na storage reaction with a high specific capacity of 150 mAh g(-1) and a ∼ 90% capacity retention over 200 cycles, possibly serving as a low cost and high performance cathode for Na-ion batteries. In particular, the synthetic strategy described in this work may be extended to other coordination-framework materials for a wide range of energy conversion and storage applications.
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http://dx.doi.org/10.1021/acsami.5b12620DOI Listing
March 2016

X-ray Absorption Spectra of Dissolved Polysulfides in Lithium-Sulfur Batteries from First-Principles.

J Phys Chem Lett 2014 May 14;5(9):1547-51. Epub 2014 Apr 14.

⊥Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, United States.

The X-ray absorption spectra (XAS) of lithium polysulfides (Li2Sx) of various chain lengths (x) dissolved in a model solvent are obtained from first-principles calculations. The spectra exhibit two main absorption features near the sulfur K-edge, which are unambiguously interpreted as a pre-edge near 2471 eV due to the terminal sulfur atoms at either end of the linear polysulfide dianions and a main-edge near 2473 eV due to the (x - 2) internal atoms in the chain, except in the case of Li2S2, which only has a low-energy feature. We find an almost linear dependence between the ratio of the peaks and chain length, although the linear dependence is modified by the delocalized, molecular nature of the core-excited states that can span up to six neighboring sulfur atoms. Thus, our results indicate that the ratio of the peak area, and not the peak intensities, should be used when attempting to differentiate the polysulfides from XAS.
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http://dx.doi.org/10.1021/jz500260sDOI Listing
May 2014
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