Publications by authors named "Chengcheng Li"

179 Publications

Sema4C modulates the migration of primary tumor-associated lymphatic endothelial cells via an ERK-mediated pathway.

Exp Ther Med 2021 Oct 2;22(4):1102. Epub 2021 Aug 2.

Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250001, P.R. China.

Although lymphatic endothelial cells (LECs) serve a positive role in tumor lymphatic metastasis, the regulation of LECs undergoing migration similar to that of tumor cells remains poorly understood. A previous study revealed that semaphorin 4C (Sema4C) could be a marker of LECs in cervical cancer. Thus, the present study aimed to understand the mechanism via which Sema4C could promote the development of tumor-associated characteristics in LECs in cervical cancer. Primary tumor-associated LECs (TLECs) were distinguished from cervical cancer by flow cytometry. The promigratory ability was assessed using the Transwell assay. Lentivirus infection was used to alter the expression of Sema4C in TLECs. Confocal laser scanning was used to determine the infection efficiency of lentivirus infection. Sema4C/ERK/E-cadherin pathway was measured by reverse transcription-quantitative PCR and western blotting. The co-localization of Sema4C and the lymphatic marker lymphatic vessel endothelial hyaluronan receptor 1 was verified. Primary tumor-associated LECs (TLECs) were isolated from a mouse xenograft cervical tumor model. It was revealed that overexpressing Sema4C stimulated the migratory ability of TLECs, downregulated E-cadherin expression and stimulated ERK phosphorylation, whereas knocking down Sema4C had the opposite effects. The treatment of PD98059 (ERK inhibitor) blocked the pro-migratory ability of TLECs, which indicated a dependence on the ERK signaling pathway. It was identified that the Sema4C/ERK/E-cadherin pathway may be critical for the migration of TLECs, which may promote lymph node metastasis. Therefore, Sema4C could be a promising target for the treatment of cervical cancer with lymphatic metastasis.
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http://dx.doi.org/10.3892/etm.2021.10535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383750PMC
October 2021

Gratitude and depressive symptoms in Chinese nurses: A longitudinal cross-lagged study.

Appl Psychol Health Well Being 2021 Sep 8. Epub 2021 Sep 8.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Nurses have a relatively higher rate of depressive symptoms compared to other people. Previous studies have reported that gratitude is correlated with depressive symptoms among healthcare workers. The purpose of this study was to explore the causal relationship between gratitude and depressive symptoms among Chinese nurses. We collected data over 4 months, with 393 nurses at Time 1 and 241 at Time 2. All participants completed online questionnaires. We employed the structural equation models to test the hypotheses. The results revealed that gratitude at Time 1 could predict depressive symptoms at Time 2, and depressive symptoms at Time 1 could predict gratitude at Time 2. These results remained significant after controlling for gender, age, education, marital status, professional title, general positive affect, and negative affect. The findings provide initial evidence about the causal relationship between gratitude and depressive symptoms among Chinese nurses. Hospital administrators can intervene in depressive symptoms of nurses by developing gratitude interventions. Furthermore, nurses with lower levels of depressive symptoms feel more grateful.
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http://dx.doi.org/10.1111/aphw.12305DOI Listing
September 2021

TGFBR2 mutation predicts resistance to immune checkpoint inhibitors in patients with non-small cell lung cancer.

Ther Adv Med Oncol 2021 14;13:17588359211038477. Epub 2021 Aug 14.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Pan-jia-yuan South Lane, Chaoyang District, Beijing, 100021, China.

Background: Resistance or even hyper-progression to immune checkpoint inhibitors (ICIs) manifesting as accelerated disease progression or death has impeded the clinical use of ICIs. The transforming growth factor beta (TGFβ) receptor pathway has been identified in contributing to immune dysfunction, which might be associated with resistance to ICIs. We aimed to explore the role of TGFβ in the resistance to ICIs in non-small cell lung cancer (NSCLC) in this study.

Methods: Public cohorts with patients treated with ICIs or chemotherapy including POPLAR/OAK ( = 853), MSKCC ( = 1662) and Van Allen ( = 57) and TCGA ( = 3210) cohorts were obtained and analyzed.

Results: The expression of immune-checkpoint related genes, including (), (), (TIGIT), (), (), and () were significantly upregulated in transforming growth factor beta TGFβ receptor 2 (TGFβR2)-mutated patients than those with wild-type TGFBR2 ( < 0.05). In the POPLAR/OAK cohort, TGFBR2-mutated patients showed shorter progression-free survival (PFS) [  = 0.004; hazard ratio (HR), 2.83; 95% confidence interval (CI), 1.34-6.00] and overall survival (OS) (  = 0.0006; HR, 3.46; 95% CI, 1.63-7.35) than those with wild-type TGFBR2 when treated with ICIs but not chemotherapy. In the merged MSKCC and Van Allen cohorts, a similar result was observed that the OS was inferior in patients with mutated TGFBR2 compared with those with wild-type TGFBR2 ( = 0.007; HR, 2.53; 95% CI, 1.25-5.12). The association between TGBFR2 mutation and survival remained significant in multivariable cox regression in both POPLAR/OAK cohort ( = 0.02; HR, 2.53; 95% CI, 1.17-5.45) and merged cohort ( = 0.008; HR, 2.63; 95% CI, 1.29-5.35). We further evaluated the association between TGFBR2 mutations and OS in multiple types of tumors. The association between TGFBR2 mutations and OS remained significant in NSCLC ( = 0.02; HR, 2.47; 95% CI, 1.16-5.26), but not in other type of tumors.

Conclusions: We identified that TGFBR2 mutation predicted the resistance to ICIs in NSCLCs. The clinical delivery of ICIs should be cautious in those patients.
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http://dx.doi.org/10.1177/17588359211038477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366138PMC
August 2021

Assessment of shallow aquifer vulnerability to fluoride contamination using modified AHP-DRASTICH model as a tool for effective groundwater management, a case study in Yuncheng Basin, China.

Chemosphere 2021 Jul 28;286(Pt 2):131601. Epub 2021 Jul 28.

State Key Laboratory of Biogeology and Environmental Geology and School of Environmental Studies, China University of Geosciences, Wuhan, 430074, PR China. Electronic address:

Vulnerability assessment is an effective tool for the precaution of groundwater quality to specific pollutants. Groundwater fluoride pollution is severe and universal in the world, however, the method of groundwater risk assessment to fluorine is never built. The objective of this study was to establish an effective method to assess the potential level of groundwater vulnerability to fluoride by a modification of the typical DRASTIC model. The hydrogeochemical (H) factor (pH and TDS) was designed as a complementary parameter in the DRASTICH model. The weights of the parameters were revised by Analytical Hierarchy Process (AHP) method. The built AHP-DRASTICH model was applied to evaluate the groundwater vulnerability to fluoride contamination in Yuncheng basin, northern China, where groundwater with high fluoride concentration occurred extensively. The assessment result indicates that about 40 percent of the area belonged to relatively high and high vulnerability zones, which mainly distributed at the central part of the basin with strong evaporation and longer water-rock interaction time. The AHP-DRASTICH model shows a stronger positive correlation between risk scores and F concentration, giving better vulnerability assessment to F pollution compared with DARSTIC and DRASTICH models. The AHP-DRASTICH model is reliable and useful for guiding government and policy maker to take effective actions protecting groundwater from specific pollution.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131601DOI Listing
July 2021

Functionalized Masks: Powerful Materials against COVID-19 and Future Pandemics.

Small 2021 Jul 28:e2102453. Epub 2021 Jul 28.

Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick, E3B 5A3, Canada.

The outbreak of COVID-19 revealed the vulnerability of commercially available face masks. Without having antibacterial/antiviral activities, the current masks act only as filtering materials of the aerosols containing microorganisms. Meanwhile, in surgical masks, the viral and bacterial filtration highly depends on the electrostatic charges of masks. These electrostatic charges disappear after 8 h, which leads to a significant decline in filtration efficiency. Therefore, to enhance the masks' protection performance, fabrication of innovative masks with more advanced functions is in urgent demand. This review summarizes the various functionalizing agents which can endow four important functions in the masks including i) boosting the antimicrobial and self-disinfectant characteristics via incorporating metal nanoparticles or photosensitizers, ii) increasing the self-cleaning by inserting superhydrophobic materials such as graphenes and alkyl silanes, iii) creating photo/electrothermal properties by forming graphene and metal thin films within the masks, and iv) incorporating triboelectric nanogenerators among the friction layers of masks to stabilize the electrostatic charges and facilitating the recharging of masks. The strategies for creating these properties toward the functionalized masks are discussed in detail. The effectiveness and limitation of each method in generating the desired properties are well-explained along with addressing the prospects for the future development of masks.
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http://dx.doi.org/10.1002/smll.202102453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420174PMC
July 2021

Soil amendments inhibited the cadmium accumulation in Ligusticum striatum DC. and improved the plant growth.

Environ Sci Pollut Res Int 2021 Jul 15. Epub 2021 Jul 15.

School of Environmental and Safety Engineering, Changzhou University, Changzhou, 213164, China.

Soil aggregates display a significant influence on the bioavailability of heavy metals in soil. In this study, we conducted a field experiment in the main producing area of Ligusticum striatum DC. to explore the effects of the amendments on cadmium (Cd) distribution in soil aggregates and plant growth. L. striatum was planted in natural Cd-polluted soils added with mixed amendments, composed of heavy/light calcium carbonate (Type 1/Type 2 amendments), calcium-bentonite, potassium dihydrogen phosphate, biochar, sodium silicate, and attapulgite, with the application rate of 0.5 t ha, 1.5 t ha, and 5.0 t ha. The results demonstrated that the application of the amendments promoted the formation of soil macroaggregates (250-2000 μm and >2000 μm) and, altered soil Cd distribution among aggregates fractions by translocating Cd from macroaggregates into small one (microaggregate; <250 μm). Soil amendments addition greatly alleviated the phytotoxic effects of Cd on plants and promoted the biomass of the rhizome of L. striatum by 14.38-53.47%. Based on the structural equation modeling, the decrease of available Cd in the fraction of large macroaggregates greatly contributed to the less accumulation of Cd in plants (r = 0.70; p < 0.05). In general, the amendments inhibited the plant Cd accumulation by re-distribution of Cd among soil aggregates and, improved the plant growth by supplying available nutrients.
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http://dx.doi.org/10.1007/s11356-021-15332-0DOI Listing
July 2021

Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.

Mikrochim Acta 2021 07 6;188(8):245. Epub 2021 Jul 6.

School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning Province, People's Republic of China.

The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs). The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes. The retention mechanisms of these compounds were discussed and confirmed by combining molecular simulations in detail. It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers. The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability. In addition, the composition of the mobile phase also had a crucial effect. Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use. Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled. The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.
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http://dx.doi.org/10.1007/s00604-021-04899-7DOI Listing
July 2021

An association between PM and pediatric respiratory outpatient visits in four Chinese cities.

Chemosphere 2021 Oct 18;280:130843. Epub 2021 May 18.

China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, China. Electronic address:

Background: The effects of exposure to particulate matter with aerodynamic diameter less than 2.5 μm (PM) on children's respiratory system were investigated in numerous epidemiological literatures. However, studies on the association between PM and pediatric outpatient visits for respiratory diseases, especially considering the multicenter studies were limited in China.

Objectives: To study the association between the short-term exposure to PM and the number of children's outpatient visits for respiratory diseases in four Chinese cities as well as the pooled health effects.

Methods: Data of pediatric outpatient visits for respiratory diseases (RD, ICD: J00-J99) from representative hospitals in Shijiazhuang (SJZ), Xi'an (XA), Nanjing (NJ) and Guangzhou (GZ) in China from 2015 to 2018 were collected and the air quality data for the same period were collected from environmental protection departments. Generalized additive model (GAM) with quasi-Poisson regression was conducted to analyze the effects of PM on the number of pediatric outpatient visits in each city. Single-day lag model (lag0 to lag7) and moving average lag model (lag01 and lag07) were used to examine the lag effects and cumulative effects. Random-effects meta-analysis was used to pool the estimated risks of four cities. The interactions between PM and temperature were also explored.

Results: The average daily/total outpatient visits for RD, in SJZ, XA, NJ and GZ from 2015 to 2018 were 854.2/1,245,384, 2353.9/3,439,025, 1267.2/1,851,438 and 1399.5/2,044,740 respectively. The percentages of acute upper respiratory infections (URD, ICD: J00-J06) and other acute lower respiratory infections (LRD, ICD: J20-J22) in RD were 33%, 13% (SJZ), 43%, 32% (XA), 26%, 21% (NJ) and 54%, 26% (GZ). The largest pooled estimates of single-day lag effects for RD, URD, and LRD were at lag0, lag0 and lag1. Every 10 μg/m increase in PM concentration was associated with a 0.46% (95%CI: 0.21%-0.70%), 0.50% (95%CI: 0.19%-0.81%) and 0.42% (95%CI: 0.06%-0.79%) increased number of outpatient visits significantly. While max cumulative effects which were all at lag 07 were 1.10% (95%CI: 0.46%-1.74%), 0.96% (95%CI: 0.20%-1.73%) and 1.06% (95%CI: 0.12%-2.00%). Less polluted cities (GZ and NJ) showed greater city-specific excess risks, but the excess risks significantly decreased after adjusting for NO in two-pollutant models. Generally, PM showed larger health hazards on lower temperature days.

Conclusions: Our study showed that exposure to the ambient PM was associated with the increase of the number of outpatient visits with pediatric respiratory diseases in four Chinese cities. The health effects of PM may not be independent of other air pollutants and could be modified by temperature.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130843DOI Listing
October 2021

Human biomonitoring of toxic and essential metals in younger elderly, octogenarians, nonagenarians and centenarians: Analysis of the Healthy Ageing and Biomarkers Cohort Study (HABCS) in China.

Environ Int 2021 11 19;156:106717. Epub 2021 Jun 19.

China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing, China; School of Public Health, Anhui Medical University, Hefei, Anhui, China. Electronic address:

Background: Metals can be either toxic or essential to health, as they play different role in oxidative stress and metabolic homeostasis during the ageing process. Population-based biomonitoring have documented levels and ranges in concentrations among general population of 0-79 years of age. In people aged 80 and above, toxic metals and essential metals may have different risk profiles, and thus need to be better studied.

Objective: Our aim is to investigate concentrations of toxic metals (arsenic, cadmium, lead and mercury) and essential metals (chromium, cobalt, molybdenum, manganese, nickel and selenium) and their role in diseases, nutritional status among younger elderly, octogenarians, nonagenarians and centenarians.

Methods: A total of 932 younger elderly, 643 octogenarians, 540 nonagenarians, 386 centenarians were included from the cross-sectional Healthy Aging and Biomarkers Cohort Study in 2017-2018. Blood or urine biological substrates were collected from each participant to determine the concentrations of toxic metals and essential metals by inductively coupled plasma mass spectrometry. Random forest was constructed to rank the importance of toxic metals and essential metals in longevity. LASSO penalized regressions were performed to select the most significant metals associated with diseases and nutritional status, of which simultaneously included all metals and adjusted for the confounding factors.

Results: Compared to women, we found higher biomarker concentrations in men for toxic metals (41.2 µg/L vs 34.4 µg/L for blood lead, 1.56 µg/L vs 1.19 µg/L for blood mercury) and lower concentration of essential metals (0.48 µg/L vs 0.58 µg/L for blood molybdenum, 10.0 µg/L vs 11.1 µg/L for blood manganese). These factors may contribute to gender difference observed in longevity, that women live longer than men. Blood lead and urine cadmium tended to increase with age (P <0.001); blood cobalt, molybdenum, manganese increased with age, blood selenium decreased with age while the prevalence of selenium deficiency was extremely low in centenarians. Among toxic metals and essential metals, LASSO penalized regression identified the most significant metals associated with chronic kidney disease was cadmium and arsenic; and it was manganese, cobalt, and selenium for diabetes; it was selenium, molybdenum, lead for anemia; it was mercury for underweight. In random forest model, the top four important metals in longevity were selenium, arsenic, lead and manganese both in men and women.

Conclusions: Generally, toxic metals levels were significantly higher while essential metals were relatively sufficient in Chinese centenarians. Toxic metals and essential metals played different role in diseases, nutritional status and longevity in the process of aging. Our research provided real world evidence of biomonitoring reference values to be used for the ongoing population health surveillance in longevity.
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http://dx.doi.org/10.1016/j.envint.2021.106717DOI Listing
November 2021

A comparison expression analysis of CXCR4, CXCL9 and Caspase-9 in dermal vascular endothelial cells between keloids and normal skin on chemotaxis and apoptosis.

J Plast Surg Hand Surg 2021 Jun 10:1-10. Epub 2021 Jun 10.

The Sixteenth Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, China.

This present study was designed to explore key biological characteristics and biomarkers associated with dermal vascular endothelial cells of keloids. GSE121618 dataset was downloaded in the Gene Expression Omnibus (GEO) Database, including the KECs group and NVECs group. Through GEO2R, we have screened the differentially expressed genes (DEGs) and performed gene ontology (GO), Gene Set Enrichment Analysis (GSEA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Then, we constructed a protein-protein interaction (PPI) network and analyzed hub genes via the Search Tool for the Retrieval of Interacting Genes (STRING) Online Database and Cytoscape software. Furthermore, experiments were performed to validate the expression of selected genes, including H&E staining, immunohistochemical staining, Western blot, and RT-qPCR. A total of 1040 DEGs were selected with GEO2R online tools. Most of the enriched pathways and processes focus on cell migration, tube development, chemotaxis, cell motility, and regulation of apoptosis. With the assistance of STRING and Cytoscape, hub genes were selected. In our validation experiments of RT-qPCR, the mRNA expression of selected genes has significant differences between different groups in tissue and cell experiments. As was shown in immunohistochemical staining, the proteins of CXCR4, CXCL9, and Caspase-9 had higher expression levels in tissue samples of the Keloid group than the Normal skin group. Western blot and RT-qPCR in dermal vascular endothelial cell experiments were consistent with the aforementioned results. This study has provided a deeper analysis of the pathogenesis of dermal vascular endothelial cells in keloids. Genes of CXCR4, CXCL9, and Caspase-9 may influence the processes of inflammatory responses and vascular endothelial cell apoptosis to exert crucial effects in the development of keloids. : GEO: gene expression omnibus; DEGs: differentially expressed genes; KVECs: keloid vascular endothelial cells; NVECs: normal skin vascular endothelial cells; GO: gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; PPI: protein protein interaction; BP: biological process; CC: cellular component; MF: molecular function; GSEA: gene set enrichment analysis; STRING: search tool for the retrieval of interacting genes; MCODE: molecular complex detection.
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http://dx.doi.org/10.1080/2000656X.2021.1934843DOI Listing
June 2021

Enhancing the Radical Scavenging Activity and UV Resistance of Lignin Nanoparticles via Surface Mannich Amination toward a Biobased Antioxidant.

Biomacromolecules 2021 06 2;22(6):2693-2701. Epub 2021 Jun 2.

The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi 214122, China.

In recent years, lignin specific activities, such as antioxidation and antibacterial and anti-ultraviolet performance, have drawn more and more attention. Nevertheless, the insufficient radical scavenging (antioxidation) activity has become one of the main drawbacks that limits its high-value application. In this study, lignin nanoparticles (LNPs) were prepared via a facile acid treatment strategy. Subsequently, surface amination of LNPs (a-LNPs) was carried out through the Mannich reaction. Specifically, the antioxidant behavior of LNPs and modified LNPs was evaluated by DPPH/DMPO radical scavenging and HeLa cell reactive oxygen species (ROS) scavenging tests, which demonstrated that the antioxidation activity of a-LNPs was more evident than that of both LNPs and butylated hydroxytoluene (BHT) commercial antioxidant. The mechanism of the radical scavenging ability of aminated LNPs was elucidated and proved to be related to the bond dissociation enthalpy of Ar-O···H, determined by the electron-donating effect of the substituted groups in the -position. Meanwhile, the morphologies, solubilities, and UV-absorbing and antibacterial behavior of LNPs and a-LNPs were also studied, and the results showed that a-LNP sample exhibited higher UV resistance performance than LNPs. We expected that the modified LNPs with high antioxidation activity can serve as a safe and lower-cost biobased antioxidant.
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http://dx.doi.org/10.1021/acs.biomac.1c00387DOI Listing
June 2021

Invited Response on: Comment on Balancing Nipple and Inframammary Fold in Transaxillary Augmentation Mammaplasty with Anatomical Implant: The 'NIMF' Classification and Surgical Algorithm.

Aesthetic Plast Surg 2021 08 1;45(4):1943-1945. Epub 2021 Jun 1.

Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 33 Badachu Road, Shijingshan District, Beijing, 100144, China.

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http://dx.doi.org/10.1007/s00266-021-02374-5DOI Listing
August 2021

Investigation on many-body effects in micro-LEDs under ultra-high injection levels.

Opt Express 2021 Apr;29(9):13219-13230

Micro-LEDs can work under an extremely high injection level and are widely used in high-brightness micro-displays and visible light communication. With the increase of carrier concentration, many-body effects gradually become important factors affecting devices' characteristics. Considering the effects of carrier scattering, bandgap renormalization, and Coulomb enhancement (CE), changes in the electroluminescence spectra of micro-LEDs are analyzed as the current density increases from 49.2 to 358.2 kA/cm, the latter representing an ultra-high injection level. Affected by plasma screening, CE decreases below about 150 kA/cm. After that, polarization screening dominates and effectively alleviates the spatial separation of electrons and holes, which results in CE increases to the maximum injection level of 358.2 kA/cm. It is established that CE promotes radiative recombination processes. Different from the traditional phenomenon of "efficiency droop", the enhanced attraction between carriers leads to an abnormal increase of external quantum efficiency at high current density.
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http://dx.doi.org/10.1364/OE.422151DOI Listing
April 2021

Gut Structure and Microbial Communities in (Hymenoptera: Siricidae) and Their Predicted Contribution to Larval Nutrition.

Front Microbiol 2021 8;12:641141. Epub 2021 Apr 8.

Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, China.

The European woodwasp, Fabricius, is a major invasive quarantine pest that attacks and kills pine trees outside of its native range. Insect gut structure and gut microbiota play crucial roles in various life activities. Despite a few reports in nutrition and survival, an extensive study on the larval gut microbiome is lacking. We studied the gut structure using a stereo microscope and used high throughput sequencing of the bacterial 16S rRNA genes and fungal internal transcribed spacer 2 (ITS2) regions to investigate gut microbiota in different developmental stages of , including larvae, adults, and larval frass. We used PICRUSt2 to predict the functional profiles. The larval gut was thin and thread-like from the oral cavity to the anus, carrying few xylem particles in the crop. , , and s.l were the dominant bacteria in the guts of larvae, adults, and frass, respectively. Even though was the most abundant among all bacteria, , , and , which might be involved in degrading organic matter and fixing nitrogen occurred exclusively in the larval gut indicating their possible role in the growth and development of larvae in pine tree xylem. Fungal communities did not change significantly across different developmental stages or the frass. was dominant in the woodwasp's larval gut. Functional prediction of bacterial and fungal communities revealed that they may encod enzymes involved in degrading lignocellulose and fixing nitrogen. Ours is the first study that compares gut microbial communities present in larvae, adults, and frass. This study could provide an understanding of larval nutrient acquisition in nutrient-deficient host xylem to some extent. Our study may unlock novel strategies for the development of pest management approaches based on interfering with the gut microbiota and restricting their role in larval survival and development.
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http://dx.doi.org/10.3389/fmicb.2021.641141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060704PMC
April 2021

Naturally Occurring Exopolysaccharide Nanoparticles: Formation Process and Their Application in Glutathione Detection.

ACS Appl Mater Interfaces 2021 May 21;13(17):19756-19767. Epub 2021 Apr 21.

Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.

Naturally occurring nanoscale exopolysaccharide (EPS) has attracted much attention in recent years. In this research, we obtained a new kind of naturally occurring spherical EPS nanoparticles (EPS-R503) from R503. The secretion, self-assembly process, morphological structure, and surface characteristics of the as-prepared nanoparticles were comprehensively revealed with transmission electron microscopy (TEM) and atomic force microscope (AFM) for the first time. It was found that the EPS-R503 nanoparticles consist of negatively charged heteropolysaccharide composed of mannose, glucose, galactose, and glucuronide with several functional groups including -OH, -COOH, and -NH. When different solvents were used to treat the EPS-R503 nanoparticles, the morphological structure and surface properties could be changed or manipulated. The forming mechanism of EPS-R503 was elucidated based on the aggregation processes from a fundamental point of view. Furthermore, EPS-R503 can serve as reducing and stabilizing agents for the biosynthesis of manganese dioxide nanosheets (MnO NSs), leading to EPS-MnO nanocomposite. The as-prepared nanocomposites can absorb fluorescein (FL) to form EPS-MnO-FL, which can be used to detect glutathione (GSH) with a low limit of detection (0.16 μM) and a wide detection range from 0.05 to 4 mM. The excellent biocompatibility of EPS-MnO-FL endows the feasibility of in vivo detection of GSH as well. Overall, the findings from this work not only benefit the exploitation of naturally occurring EPS nanomaterials but also provide a novel strategy for the green synthesis of metal-containing nanosheets for GSH detection.
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http://dx.doi.org/10.1021/acsami.1c03489DOI Listing
May 2021

Balancing Nipple and Inframammary Fold in Transaxillary Augmentation Mammaplasty with Anatomical Implant: The 'NIMF' Classification and Surgical Algorithm.

Aesthetic Plast Surg 2021 08 19;45(4):1497-1506. Epub 2021 Apr 19.

Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No.33 Badachu Road, Shijingshan District, Beijing, 100144, China.

Background: This study aims to put forward a new classification of breast asymmetry based on the relative position of the nipple and inframammary fold (IMF) and propose a surgical algorithm of determining new IMF to address breast asymmetry in patients undergoing transaxillary augmentation mammaplasty, which is named as NIMF (nipple inframammary) classification and surgical algorithm.

Methods: Three hundred and forty-five patients received transaxillary augmentation mammaplasty with anatomical implants. Preoperative breast asymmetry was classified into four types. I: asymmetrical nipple with asymmetrical IMF in the same direction; II: symmetrical nipple with asymmetrical IMF; III: asymmetrical nipple with symmetrical IMF; IV: asymmetrical nipple with unapparent IMF. Surgical plans (3 plans for type I, II, IV while 5 plans for type III) to set the new IMF were provided for each patient, who chose one of them as the final surgical plan. Breast-Q and Likert scale were used to evaluate patient satisfaction and symmetry of breast preoperatively and 6 months postoperatively.

Results: The incidence of type I, II, III, IV was 30%, 15%, 13%, and 4%, respectively. Ninety-seven percent of patients with breast asymmetry chose plan C, which aimed to balance the relative position of nipple and IMF. Postoperative Breast-Q scores showed a significant rise compared with preoperative scores, but no statistical difference between plan C V.S. other plans. Patients with symmetrical preoperative breasts (Group A) had significantly higher postoperative Breast-Q scores than patients with asymmetrical preoperative breasts (Group B). In breast symmetry assessment, Group A had a significantly higher postoperative score than Group B, but the postoperative score was significantly lowered compared with the preoperative score in both Group A and B.

Conclusion: The NIMF classification and surgical algorithm provide a systematic and scientific way to analyze and improve breast asymmetry, to achieve optimized patient satisfaction in transaxillary augmentation mammaplasty with anatomical implants.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02274-8DOI Listing
August 2021

Sources and pollution path identification of PAHs in karst aquifers: an example from Liulin karst water system, northern China.

J Contam Hydrol 2021 Aug 15;241:103810. Epub 2021 Apr 15.

Hubei Provincial Academy of Eco-environmental Sciences, Wuhan 430070, PR China.

Karst water, with constituting major sources for water supply worldwide, is vulnerable and prone to be polluted. In this study, it is reported that karst water polycylic aromatic hydrocarbons (PAHs) pollution is caused by the infiltration of surface runoff in the bared carbonate areas, which is of universal significance for the protection of groundwater resources in karst region. Hydro-geochemistry, stable isotopes (δD, δO and Sr/Sr) and characteristic ratio method were conducted together to illustrate the concentration, distribution, sources and pollution path of polycyclic aromatic hydrocarbons in groundwater in the Liulin karst water system of northern China. The results showed that total concentration of polycyclic aromatic hydrocarbons ranged from 39.25 to 16,830 ng/L in groundwater, with Naphthalene being the dominant component, and the median value increased gradually along the flow path. The highest polycyclic aromatic hydrocarbons concentrations in karst water were mainly observed in the coal mining and the discharge areas. Based on the characteristic ratios, the polycyclic aromatic hydrocarbons in the study area mainly come from local incomplete combustion of woods, fossil fuels, coal and liquid fuels. The slight shift of δD and δO and moderate Sr/Sr ratios suggest that the polycyclic aromatic hydrocarbons in karst water is mainly polluted by surface runoff during rain events in the bared karst region. The leakage of river water may partly contribute to the polycyclic aromatic hydrocarbons in some karst water, which normally located close to the karst water - river water mixing line. This study provides a new technical method for tracing the sources and identifying the pollution paths of organic pollution in a karst water system.
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http://dx.doi.org/10.1016/j.jconhyd.2021.103810DOI Listing
August 2021

Effective Analysis of Inpatient Satisfaction: The Random Forest Algorithm.

Patient Prefer Adherence 2021 7;15:691-703. Epub 2021 Apr 7.

School of Humanities and Social Sciences, Guangxi Medical University, Nanning, 530021, People's Republic of China.

Purpose: To identify the factors influencing inpatient satisfaction by fitting the optimal discriminant model.

Patients And Methods: A cross-sectional survey of inpatient satisfaction was conducted with 3888 patients in 16 large public hospitals in Zhejiang Province. Independent variables were screened by single-factor analysis, and the importance of all variables was comprehensively evaluated. The relationship between patients' overall satisfaction and influencing factors was established, the relative risk was evaluated by marginal benefit, and the optimal model was fitted using the receiver operating characteristic curve.

Results: Patients' overall satisfaction was 79.73%. The five most influential factors on inpatient satisfaction, in this order, were: patients' right to know, timely nursing response, satisfaction with medical staff service, integrity of medical staff, and accuracy of diagnosis. The prediction accuracy of the random forest model was higher than that of the multiple logistic regression and naive Bayesian models.

Conclusion: Inpatient satisfaction is related to healthcare quality, diagnosis, and treatment process. Rapid identification and active improvement of the factors affecting patient satisfaction can reduce public hospital operating costs and improve patient experiences and the efficiency of health resource allocation. Public hospitals should strengthen the exchange of medical information between doctors and patients, shorten waiting time, and improve the level of medical technology, service attitude, and transparency of information disclosure.
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http://dx.doi.org/10.2147/PPA.S294402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039189PMC
April 2021

Laccase immobilization onto natural polysaccharides for biosensing and biodegradation.

Carbohydr Polym 2021 Jun 19;262:117963. Epub 2021 Mar 19.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NB, E3B 5 A3, Canada.

Over the last few years, the focus of researchers have been set on enzyme engineering and enzyme immobilization technology using natural polysaccharides as promising and green supporting materials to address the challenges of free enzymes for various applications. Polysaccharides have been extensively implemented as enzyme carriers because they can be easily modified chemically according to the nature of immobilization. This process improves the stability and lifetime of laccase in catalytic reactions. Additionally, the selectivity of the enzymes can be preserved for particular application after immobilization onto polysaccharides. This review paper reveals the significance and potential of natural polysaccharides (including cellulose, chitosan, and alginate) and their composites as support materials for the laccases immobilization to expand the modified biocatalysts for industrial applications. Moreover, the roles of immobilized laccases are discussed from a fundamental point of view to elucidate their catalytic mechanisms as biocatalysts in the detection and degradation of environmental contaminants.
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http://dx.doi.org/10.1016/j.carbpol.2021.117963DOI Listing
June 2021

Indocyanine Green Angiography for Fat Necrosis Reduction in the Deep Inferior Epigastric Perforator Flap.

Plast Reconstr Surg 2021 04;147(4):701e-702e

Plastic Surgery Hospital (Institute), Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007733DOI Listing
April 2021

Activity and Resistance-Related Point Mutations in Target Protein PcORP1 of Fluoxapiprolin in .

J Agric Food Chem 2021 Apr 25;69(13):3827-3835. Epub 2021 Mar 25.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, 3 Taicheng Road, Yangling 712100, Shaanxi, People's Republic of China.

Fluoxapiprolin is a new piperidinyl thiazole isoxazoline fungicide developed by Bayer Crop Science in 2012, but the sensitivity and resistance mechanism of fluoxapiprolin are unclear. In this study, the sensitivities of 130 isolates to fluoxapiprolin were determined, and a unimodal distribution was observed with a mean EC value of 0.00043 μg/mL. Nine stable fluoxapiprolin-resistant mutants were obtained by fungicide adaption. The fitness of mutants was similar to or lower than that of the corresponding parental isolate. Seven heterozygous point mutations in the target protein PcORP1 were found in these mutants. These point mutations were confirmed in PsORP1 of homologue positions using the CRISPR/Cas9 system. G770V and N835S+I877F do confer high fluoxapiprolin resistance (resistance factor, RF > 1000), and ΔN835, N767I, and N837T+S910C lead to low resistance (RF < 100). transformants containing L733W, S768F, S768Y, Δ818/F819, N837Y, N837F, P861H, L863W, and I877Y in PsORP1 were also associated with high fluoxapiprolin resistance (RF > 100). In conclusion, the resistance risk of to fluoxapiprolin is moderate, and multiple point mutations in PcORP1 could confer different resistance levels to fluoxapiprolin.
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http://dx.doi.org/10.1021/acs.jafc.0c05119DOI Listing
April 2021

Effect of Carbon Ion Radiation Induces Bystander Effect on Metastasis of A549 Cells and Metabonomic Correlation Analysis.

Front Oncol 2020 2;10:601620. Epub 2021 Mar 2.

The Basic Medical College of Lanzhou University, Lanzhou, China.

Objective: To analyze the effect of carbon ion (C) radiation may induce bystander effect on A549 cell metastasis and metabonomics.

Methods: A549 cell was irradiated with carbon ion to establish the clone survival model and the transwell matrix assay was applied to measure the effect of carbon ion on cell viability, migration, and invasion, respectively. Normal human embryonic lung fibroblasts (WI-38) were irradiated with carbon ions of 0 and 2 Gy and then transferred to A549 cell co-culture medium for 24 h. The migration and invasion of A549 cells were detected by the Transwell chamber. The analysis of metabonomic information in transfer medium by liquid phase mass spectrometry (LC-MS), The differential molecules were obtained by principal pomponent analysis (PCA) and the target proteins of significant differences ( = 1.7 × 10) obtained by combining with the STICH database. KEGG pathway was used to analyze the enrichment of the target protein pathway.

Results: Compared with 0 Gy, the colony formation, migration, and invasion of A549 cells were significantly inhibited by carbon ion 2 and 4 Gy irradiation, while the inhibitory effect was not significant after 1 Gy irradiation. Compared with 0 Gy, the culture medium 24 h after carbon ion 2 Gy irradiation significantly inhibited the metastasis of tumor cells ( = 0.03). LC-MS analysis showed that 23 differential metabolites were obtained in the cell culture medium 24 h after carbon ion 0 and 2 Gy irradiation (9 up-regulated and 14 down-regulated). Among them, two were up-regulated and two down-regulated ( = 2.9 × 10). 41 target proteins were corresponding to these four differential molecules. Through the analysis of the KEGG signal pathway, it was found that these target molecules were mainly enriched in purine metabolism, tyrosine metabolism, cysteine and methionine metabolism, peroxisome, and carbon metabolism. Neuroactive ligand-receptor interaction, calcium signaling pathway, arachidonic acid metabolism, and Fc epsilon RI signaling pathway.

Conclusion: The bystander effect induced by 2 Gy carbon ion radiation inhibits the metastasis of tumor cells, which indicates that carbon ions may change the metabolites of irradiated cells, so that it may indirectly affect the metabolism of tumor cell growth microenvironment, thus inhibiting the metastasis of malignant tumor cells.
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http://dx.doi.org/10.3389/fonc.2020.601620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962605PMC
March 2021

Antimicrobial/Biocompatible Hydrogels Dual-Reinforced by Cellulose as Ultrastretchable and Rapid Self-Healing Wound Dressing.

Biomacromolecules 2021 04 3;22(4):1654-1663. Epub 2021 Mar 3.

Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.

Hydrogels as a wound dressing, integrated with ultrastretchability, rapid self-healing, and excellent antimicrobial activity, are in high demand, particularly for joint skin wound healing. Herein, a multifunctional and ductile composite hydrogel was developed using poly(vinyl alcohol) (PVA)-borax gel as a matrix that was synergized or dual-reinforced with dopamine-grafted oxidized carboxymethyl cellulose (OCMC-DA) and cellulose nanofibers (CNF). Moreover, neomycin (NEO), an aminoglycoside antibiotic with multifunctional groups, was incorporated into the hydrogel network as both an antibacterial agent and a cross-linker. The dynamic reversible borate ester linkages and hydrogen bonds between OCMC-DA, PVA, and CNF, along with dynamic cross-linking imine linkages between NEO and OCMC-DA, endowed the hydrogel with excellent self-healing ability and stretchability (3300%). The as-reinforced networks enhanced the mechanical properties of hydrogels significantly. More remarkably, the composite hydrogel with improved biodegradability and biocompatibility is pH-responsive and effective against a broad spectrum of bacteria, which is attributed to the controllable release of NEO for steady availability of the antibiotic on the wound location. Overall, the antimicrobial hydrogel with rapid self-healing and reliable mechanical properties holds significant promise as dressing material for wound healing.
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http://dx.doi.org/10.1021/acs.biomac.1c00086DOI Listing
April 2021

A Sequence in Recipient-Site Selection in Stacked Free Flap Breast Reconstruction: Cranial and Caudal Internal Mammary Vessels prior to Other Alternatives.

Plast Reconstr Surg 2021 03;147(3):556e-557e

Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007647DOI Listing
March 2021

Inhibition of plasma kallikrein mitigates experimental hypertension-enhanced cerebral hematoma expansion.

Brain Res Bull 2021 May 5;170:49-57. Epub 2021 Feb 5.

Department of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China; State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing, 400038, China; Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China. Electronic address:

Rationale: Hematoma expansion (HE) aggravates brain injury after intracerebral hemorrhage (ICH) and hypertension is a key contributor to HE. Plasma kallikrein (PK) is involved in hemorrhagic transformation in ischemic stroke mice. This study was conducted to explore the role of PK in HE in hypertensive ICH.

Methods: Hypertension was achieved by continuous infusion of angiotensin II (Ang II) with an osmotic pump in C57BL/6 mice. ICH was achieved by stereotactic intrastriatal injection of blood. PK-specific antibody and platelet glycoprotein VI (GPVI) agonists were administered to intervene in hematoma expansion. The hematoma volume was indicated by the erythrocyte components hemoglobin and carbonic anhydrase-1 in the ipsilateral brain hemisphere.

Results: Ang II-induced hypertensive mice showed enhanced hematoma expansion and worsened neurologic deficits after ICH modeling. Moreover, intrastriatal injection of blood from Ang II-treated mice into normal mice increased the area of secondary hemorrhage more than blood from untreated mice. Mechanistically, elevated PK was found in Ang II-infused mice whereas, inhibition of PK and administration of the GPVI agonist convulxin decreased hematoma expansion and improved neurologic deficits after ICH.

Conclusions: These findings suggest that PK inhibition and GPVI agonist treatment might serve as potential methods to intervene in HE after ICH.
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http://dx.doi.org/10.1016/j.brainresbull.2021.02.004DOI Listing
May 2021

Importance of Adipose Tissue NAD+ Biology in Regulating Metabolic Flexibility.

Endocrinology 2021 03;162(3)

Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri, USA.

Nicotinamide adenine dinucleotide (NAD+) is an essential coenzyme that regulates cellular energy metabolism in many cell types. The major purpose of the present study was to test the hypothesis that NAD+ in white adipose tissue (WAT) is a regulator of whole-body metabolic flexibility in response to changes in insulin sensitivity and with respect to substrate availability and use during feeding and fasting conditions. To this end, we first evaluated the relationship between WAT NAD+ concentration and metabolic flexibility in mice and humans. We found that WAT NAD+ concentration was increased in mice after calorie restriction and exercise, 2 enhancers of metabolic flexibility. Bariatric surgery-induced 20% weight loss increased plasma adiponectin concentration, skeletal muscle insulin sensitivity, and WAT NAD+ concentration in people with obesity. We next analyzed adipocyte-specific nicotinamide phosphoribosyltransferase (Nampt) knockout (ANKO) mice, which have markedly decreased NAD+ concentrations in WAT. ANKO mice oxidized more glucose during the light period and after fasting than control mice. In contrast, the normal postprandial stimulation of glucose oxidation and suppression of fat oxidation were impaired in ANKO mice. Data obtained from RNA-sequencing of WAT suggest that loss of NAMPT increases inflammation, and impairs insulin sensitivity, glucose oxidation, lipolysis, branched-chain amino acid catabolism, and mitochondrial function in WAT, which are features of metabolic inflexibility. These results demonstrate a novel function of WAT NAMPT-mediated NAD+ biosynthesis in regulating whole-body metabolic flexibility, and provide new insights into the role of adipose tissue NAD+ biology in metabolic health.
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http://dx.doi.org/10.1210/endocr/bqab006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853299PMC
March 2021

Discovery and validation of methylation signatures in blood-based circulating tumor cell-free DNA in early detection of colorectal carcinoma: a case-control study.

Clin Epigenetics 2021 02 3;13(1):26. Epub 2021 Feb 3.

Colorectal Surgery Department, Changhai Hospital, Naval Medical University, Changhai Road No.168, Yangpu District, Shanghai, 200433, China.

Background: Early detection of colorectal carcinoma (CRC) would help to identify tumors when curative treatments are available and beneficial. However, current screening methods for CRC, e.g., colonoscopy, may affect patients' compliance due to the uncomfortable, invasive and time-consuming process. In recent decades, methylation profiles of blood-based circulating tumor DNA (ctDNA) have shown promising results in the early detection of multiple tumors. Here we conducted a study to investigate the performance of ctDNA methylation markers in early detection of CRC.

Results: In total, 742 participants were enrolled in the study including CRC (n = 332), healthy control (n = 333), benign colorectal disease (n = 65) and advanced adenoma (n = 12). After age-matched and randomization, 298 participants (149 cancer and 149 healthy control) were included in training set and 141 (67 cancer and 74 healthy control) were in test set. In the training set, the specificity was 89.3% (83.2-93.7%) and the sensitivity was 88.6% (82.4-93.2%). In terms of different stages, the sensitivities were 79.4% (62.1-91.2%) in patients with stage I, 88.9% (77.3-95.8%) in patients with stage II, 91.4% (76.9-98.2%) in patients with stage III and 96.2% (80.3-99.9%) in patients with stage IV. Similar results were validated in the test set with the specificity of 91.9% (83.1-97.0%) and sensitivity of 83.6% (72.5-91.6%). Sensitivities for stage I-III were 87.0% (79.7-92.4%) in the training set and 82.5% (70.2-91.3%) in the test set, respectively. In the unmatched total population, the positive ratios were 7.8% (5.2-11.2%) in healthy control, 30.8% (19.9-43.5%) in benign colorectal disease and 58.3% (27.5-84.7%) in advanced adenoma, while the sensitivities of stage I-IV were similar with training and test sets. Compared with methylated SEPT9 model, the present model had higher sensitivity (87.0% [81.8-91.2%] versus 41.2% [34.6-48.1%], P < 0.001) under comparable specificity (90.1% [85.4-93.7%] versus 90.6% [86.0-94.1%]).

Conclusions: Together our findings showed that ctDNA methylation markers were promising in the early detection of CRC. Further validation of this model is warranted in prospective studies.
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http://dx.doi.org/10.1186/s13148-020-00985-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856810PMC
February 2021

Laser-assisted topical steroid application vs steroid injection for treating keloids: A split side study.

J Cosmet Dermatol 2021 Apr 2;20(4):1355-1356. Epub 2021 Feb 2.

Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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http://dx.doi.org/10.1111/jocd.13964DOI Listing
April 2021

Overwintering Larval Cold Tolerance of (Hymenoptera: Siricidae): Geographic Variation in Northeast China.

Insects 2021 Jan 28;12(2). Epub 2021 Jan 28.

Beijing Key Laboratory for Forest Pest Control, School of Forestry, Beijing Forestry University, Beijing 100083, China.

(Hymenoptera: Siricidae) is an invasive pest that has spread and established in many regions worldwide. However, its cold tolerance strategy is still unclear. We measured the supercooling point (SCP) and the lower lethal temperature (LLT) of overwintering larvae during three overwintering periods in four geographically separated populations in China. In addition, using the statistical analysis of the local historical temperature data, we also conducted comprehensive studies of cold tolerance variations and strategies. We measured the SCP of all samples as could survive at its SCP during a short period of exposure (<48 h) and its cold tolerance strategy was freeze-avoidance. The average SCPs of the groups in different spatiotemporal scales were significantly related to average temperature variation with most individuals exhibiting stronger cold hardiness at low ambient temperatures. exhibited a strong cold tolerance and it has the ability to withstand lower temperatures in cold environments. The geographic population showed a positive tendency as the ambient temperature decreased, which would increase its chance of developing in cold regions.
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http://dx.doi.org/10.3390/insects12020116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911264PMC
January 2021

Safety and Efficacy of PD-1/PD-L1 inhibitors combined with radiotherapy in patients with non-small-cell lung cancer: a systematic review and meta-analysis.

Cancer Med 2021 02 19;10(4):1222-1239. Epub 2021 Jan 19.

The First School of Clinical Medicine, Lanzhou University, Lanzhou, China.

Background: A combination of programmed cell death protein-1 (PD-1)/programmed cell death ligand-1 (PD-L1) inhibitors and radiotherapy (RT) is increasingly being used to treat non-small-cell lung cancer (NSCLC). However, the safety and efficacy of this approach remains controversial. We performed a systematic review and meta-analysis to summarize the related research.

Methods: We searched the China Biology Medicine, EMBASE, Cochrane Library, and PubMed databases for all the relevant studies. The Stata software, version 12.0 was used for the meta-analysis.

Results: The study included 20 clinical trials that enrolled 2027 patients with NSCLC. Compared with non-combination therapy, combination therapy using PD-1/PD-L1 inhibitors and RT was associated with prolonged overall survival (OS) (1-year OS: odds ratio [OR] 1.77, 95% confidence interval [CI] 1.35-2.33, p = 0.000; 2-year OS: OR 1.77, 95% CI 1.35-2.33, p = 0.000) and progression-free survival (PFS) (0.5-year PFS: OR 1.83, 95% CI 1.13-2.98, p = 0.014; 1-year PFS: OR 2.09, 95% CI 1.29-3.38, p = 0.003; 2-year PFS: OR 2.47, 95% CI 1.13-5.37, p = 0.023). Combination therapy also improved the objective response rate (OR 2.76, 95% CI 1.06-7.19, p = 0.038) and disease control rate (OR 1.80, 95% CI 1.21-2.68, p = 0.004). This meta-analysis showed that compared with non-combination therapy, combination therapy using PD-1/PD-L1 inhibitors and RT did not increase the serious adverse event rates (≥grade 3); however, this approach increased the rate of grade 1-2 immune-related or radiation pneumonitis. Subgroup analyses revealed that the sequence of PD-1/PD-L1 inhibitors followed RT outperformed in which concurrent PD-1/PD-L1 inhibitor and RT followed PD-1/PD-L1 inhibitor. Combination of stereotactic body RT or stereotactic radiosurgery with PD-1/PD-L1 inhibitors may be more effective than a combination of conventional RT with PD-1/PD-L1 inhibitors in patients with advanced NSCLC.

Conclusion: Combination therapy using PD-1/PD-L1 inhibitors and RT may improve OS, PFS, and tumor response rates without an increase in serious adverse events in patients with advanced NSCLC. However, combination therapy was shown to increase the incidence of mild pneumonitis.
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http://dx.doi.org/10.1002/cam4.3718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926021PMC
February 2021
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