Publications by authors named "Cheng Yang"

1,545 Publications

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Ecological risk assessment and identification of the distinct microbial groups in heavy metal-polluted river sediments.

Environ Geochem Health 2022 Aug 8. Epub 2022 Aug 8.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 610031, China.

To assess the health of river ecosystems, it is essential to quantify the ecological risk of heavy metals in river sediments and the structure of microbial communities. As important tributaries of the Tuo River in the upper reaches of the Yangtze River, the Mianyuan River and the Shiting River, are closely related to the economic development and human daily life in the region. This study assessed the ecological risks of heavy-metal-polluted river sediments, the heavy-metal-driven bacterial communities were revealed, and the relationships between the ecological risks and the identical bacterial communities were discussed. The Cd content was significantly greater than the environmental background value, leading to a serious pollution and very high ecological risk at the confluence of the two rivers and the upper reaches of the Mianyuan River. Microbial community analysis showed that Rhodobacter, Nocardioides, Sphingomonas, and Pseudarthrobacter were the dominant bacterial genera in the sediments of the Shiting River. However, the dominant bacterial genera in the Mianyuan River were Kouleothrix, Dechloromonas, Gaiella, Pedomicrobium, and Hyphomicrobium. Mantel test results showed (r = 0.5977, P = 0.005) that the Cd, As, Zn, Pb, Cr, and Cu were important factors that influenced differences in the distribution of sediment bacterial communities Mianyuan and Shiting rivers. A correlation heatmap showed that heavy metals were negatively correlated for most bacterial communities, but some bacterial communities were tolerant and showed a positive correlation. Overall, the microbial structure of the river sediments showed a diverse spatial distribution due to the influence of heavy metals. The results will improve the understanding of rivers contaminated by heavy metals and provide theoretical support for conservation and in situ ecological restoration of river ecosystems.
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http://dx.doi.org/10.1007/s10653-022-01343-4DOI Listing
August 2022

Nintedanib Alleviates Experimental Colitis by Inhibiting CEBPB/PCK1 and CEBPB/EFNA1 Pathways.

Front Pharmacol 2022 14;13:904420. Epub 2022 Jul 14.

The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

The super-enhancer, a cluster of enhancers with strong transcriptional activity, has become one of the most interesting topics in recent years. This study aimed to investigate pathogenic super-enhancer-driven genes in IBD and screen therapeutic drugs based on the results. In this study, through the analysis of differentially expressed genes in colitis patients from the GEO database and the analysis of the super-enhancer-associated database, we found that the super-enhancer pathogenic genes PCK1 and EFNA1 were simultaneously regulated by transcription factor CEBPB through two super-enhancers (sc-CHR20-57528535 and sc-CHR1-155093980). Silencing CEBPB could significantly inhibit the expression of PCK1 and EFNA1 and enhance the expression of epithelial barrier proteins claudin-1, occludin, and ZO-1. In LPS-induced Caco-2 cells, drugs commonly used in clinical colitis including tofacitinib, oxalazine, mesalazine, and sulfasalazine inhibited mRNA levels of CEBPB, PCK1, and EFNA1. In the drug screening, we found that nintedanib significantly inhibited the mRNA and protein levels of CEBPB, PCK1, and EFNA1. experiments, nintedanib significantly alleviated DSS-induced colitis in mice by inhibiting CEBPB/PCK1 and CEBPB/EFNA1 signaling pathways. At the genus level, nintedanib improved the composition of the gut microbiota in mice with DSS-induced experimental colitis. In conclusion, we found that PCK1 and EFNA1 are highly expressed in colitis and they are regulated by CEBPB through two super-enhancers, and we further demonstrate their role and . Nintedanib may be a potential treatment for IBD. Super-enhancers may be a new way to explore the pathogenesis of colitis.
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http://dx.doi.org/10.3389/fphar.2022.904420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331303PMC
July 2022

Self-assembly of gelatin and phycocyanin for stabilizing thixotropic emulsions and its effect on 3D printing.

Food Chem 2022 Jul 18;397:133725. Epub 2022 Jul 18.

College of Food Science, Southwest University, Chongqing 400715, PR China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, PR China; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing 400715, PR China. Electronic address:

Nutritional phycocyanin (PC) may be non-covalently bound to gelatin (GE) and form the self-assembly complex proteins, which could stabilize high internal phase emulsions (HIPEs) by one-pot homogenization. The effects of PC on physicochemical, structural, extrudable, thixotropic properties and practical printability of HIPEs were investigated. The electrostatic interaction and hydrogen bonds between GE and PC facilitated the compact structure, promoted the interfacial adsorption behavior at oil-water interface, enhanced emulsion stability, and reduced creaming index of HIPEs. Shearing-thinning property and proper yield stress proved the excellent extrudability of HIPEs. Moreover, thixotropy results indicated that low-content PC resulted in high hysteresis area and large recovery rate of HIPEs, suggesting the outstanding structure rebuilding capacity and structure maintainability. 3D printing of HIPEs illustrated the high printing definition and shape retention conforming to the original models. Overall, this study provides reference for developing functional thixotropic emulsions with high potential in customizing special three-dimensional food.
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http://dx.doi.org/10.1016/j.foodchem.2022.133725DOI Listing
July 2022

Experimental Investigation of Relationship between Humidity Conditions and Degradation of Key Specifications of 0.1-1.2 GHz PA in 0.18 μm CMOS.

Micromachines (Basel) 2022 Jul 22;13(8). Epub 2022 Jul 22.

School of Microelectronics, Tianjin University, Tianjin 300072, China.

The specification of power amplifiers (PA) is closely related to humidity variation, and few reports on the humidity properties of PA are available in the literature. Therefore, an experimental study of PA specifications was conducted under different humidity conditions to elucidate the relationship between the degradation of PA specifications and humidity conditions. This paper studies and provides results of the degradation of a PA subjected to different humidity levels. The experimental results show that the S and output power decrease with the increase in humidity. The main cause of this degradation is the decrease in oxide capacitance and increase in threshold voltage with increasing humidity, resulting in a reduction of transconductance and an increase in on-resistance. The results of this study can guide designers in designing compensation circuits to achieve some degree of compensation for the degradation of PA specifications.
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http://dx.doi.org/10.3390/mi13081162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329907PMC
July 2022

Design of Metal-Based Slippery Liquid-Infused Porous Surfaces (SLIPSs) with Effective Liquid Repellency Achieved with a Femtosecond Laser.

Micromachines (Basel) 2022 Jul 22;13(8). Epub 2022 Jul 22.

State Key Laboratory for Manufacturing System Engineering and Shaanxi Key Laboratory of Photonics Technology for Information, School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Slippery liquid-infused porous surfaces (SLIPSs) have become an effective method to provide materials with sliding performance and, thus, achieve liquid repellency, through the process of infusing lubricants into the microstructure of the surface. However, the construction of microstructures on high-strength metals is still a significant challenge. Herein, we used a femtosecond laser with a temporally shaped Bessel beam to process NiTi alloy, and created uniform porous structures with a microhole diameter of around 4 µm, in order to store and lock lubricant. In addition, as the lubricant is an important factor that can influence the sliding properties, five different lubricants were selected to prepare the SLIPSs, and were further compared in terms of their sliding behavior. The temperature cycle test and the hydraulic pressure test were implemented to characterize the durability of the samples, and different liquids were used to investigate the possible failure under complex fluid conditions. In general, the prepared SLIPSs exhibited superior liquid repellency. We believe that, in combination with a femtosecond laser, slippery liquid-infused porous surfaces are promising for applications in a wide range of areas.
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http://dx.doi.org/10.3390/mi13081160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332264PMC
July 2022

Support Vector Machine-Based Model for 2.5-5.2 GHz CMOS Power Amplifier.

Micromachines (Basel) 2022 Jun 27;13(7). Epub 2022 Jun 27.

School of Microelectronics, Tianjin University, Tianjin 300072, China.

A power amplifier (PA) is the core module of the wireless communication system. The change of its specification directly affects the system's performance and may even lead to system failure. Furthermore, change in the PA specification is closely related to changes in temperature. To study the influence of PA specification change on the system, we used a support vector machine (SVM) to model the temperature characteristics of PA. For SVM modeling, the question of how much experimental data should be used for modeling to meet the requirements is a constant problem. To address this issue, we investigate the effect of different amounts of training data on the modeling of SVM models. The results show that only 75% of the experimental data needs to be used in the modeling process to satisfy the modeling requirements of the SVM model. The number of measurement points required in the PA specification degradation experiment can be reduced by 25%. The results of this paper serve as a guide for planning the number of experimental measurement points and reducing the measurement cost and measurement time.
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http://dx.doi.org/10.3390/mi13071012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325302PMC
June 2022

Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish ().

Int J Mol Sci 2022 Jul 12;23(14). Epub 2022 Jul 12.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Mandarin fish has an XX/XY sex-determination system. The female mandarin fish is typically larger than the male. Sex identification and the discovery of genes related to sex determination in mandarin fish have important theoretical significance in the elucidation of the regulation and evolutionary mechanism of animal reproductive development. In this study, the chromosome-level genome of a female mandarin fish was assembled, and we found that LG24 of the genome was an X chromosome. A total of 61 genes on the X chromosome showed sex-biased expression. Only six gonadal genes (LG24G00426, LG24G003280, LG24G003300, LG24G003730, LG24G004200, and LG24G004770) were expressed in the testes, and the expression of the other gene LG24G003870 isoform 1 in the ovaries was significantly higher than that in the testes ( < 0.01). Five (except LG24G003280 and LG24G003300) of the seven aforementioned genes were expressed at the embryonic development stage, suggesting their involvement in early sex determination. The expression of LG24G004770 (encoding HS6ST 3-B-like) was also significantly higher in female muscles than in male muscles ( < 0.01), indicating other functions related to female growth. ZP3 encoded by LG24G003870 isoform 1 increased the C-terminal transmembrane domain, compared with that encoded by other fish zp3 isoforms, indicating their different functions in sex determination or differentiation. This study provides a foundation for the identification of sex-determining genes in mandarin fish.
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http://dx.doi.org/10.3390/ijms23147692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321114PMC
July 2022

The Impact of CSR Perceptions on Employees' Turnover Intention during the COVID-19 Crisis in China.

Int J Environ Res Public Health 2022 Jul 7;19(14). Epub 2022 Jul 7.

Department of Media and Communication, City University of Hong Kong, Hong Kong, China.

The COVID-19 pandemic has created tremendous challenges for organizations' corporate social responsibility (CSR), communication, and relationship management with internal stakeholders such as employees. This study conducted an online survey of 466 employees working for large Chinese corporations during the pandemic. A structural equation model based on insights from expectancy violation theory was used to examine how negative violation valence increases employees' turnover intention as mediated by uncertainty, CSR cynicism, and distrust. The survey results showed that employees' negative violation valence positively influenced their uncertainty about their organizations' CSR activities, which fostered their cynicism about CSR and distrust of their organization. Employees' CSR cynicism increased their distrust toward their organizations, which increased their turnover intention. The theoretical and practical implications of the study are discussed as well.
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http://dx.doi.org/10.3390/ijerph19148297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318882PMC
July 2022

Electron delocalization triggers nonradical Fenton-like catalysis over spinel oxides.

Proc Natl Acad Sci U S A 2022 Aug 25;119(31):e2201607119. Epub 2022 Jul 25.

Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei 230026, China.

Nonradical Fenton-like catalysis offers opportunities to overcome the low efficiency and secondary pollution limitations of existing advanced oxidation decontamination technologies, but realizing this on transition metal spinel oxide catalysts remains challenging due to insufficient understanding of their catalytic mechanisms. Here, we explore the origins of catalytic selectivity of Fe-Mn spinel oxide and identify electron delocalization of the surface metal active site as the key driver of its nonradical catalysis. Through fine-tuning the crystal geometry to trigger Fe-Mn superexchange interaction at the spinel octahedra, ZnFeMnO with high-degree electron delocalization of the Mn-O unit was created to enable near 100% nonradical activation of peroxymonosulfate (PMS) at unprecedented utilization efficiency. The resulting surface-bound PMS* complex can efficiently oxidize electron-rich pollutants with extraordinary degradation activity, selectivity, and good environmental robustness to favor water decontamination applications. Our work provides a molecule-level understanding of the catalytic selectivity and bimetallic interactions of Fe-Mn spinel oxides, which may guide the design of low-cost spinel oxides for more selective and efficient decontamination applications.
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http://dx.doi.org/10.1073/pnas.2201607119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351537PMC
August 2022

Mechanistic elucidation of freezing-induced surface decomposition of aluminum oxyhydroxide adjuvant.

iScience 2022 Jun 23;25(6):104456. Epub 2022 May 23.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.

The freezing-induced aggregation of aluminum-based (Alum) adjuvants has been considered as the most important cause of reduced vaccine potency. However, the intrinsic properties that determine the functionality of Alum after freezing have not been elucidated. In this study, we used engineered aluminum oxyhydroxide nanoparticles (AlOOH NPs) and demonstrated that cryogenic freezing led to the mechanical pressure-mediated reduction of surface hydroxyl. The sugar-based surfactant, octyl glucoside (OG), was demonstrated to shield AlOOH NPs from the freezing-induced loss of hydroxyl content and the aggregation through the reduction of recrystallization-induced mechanical stress. As a result, the antigenic adsorption property of frozen AlOOH NPs could be effectively protected. When hepatitis B surface antigen (HBsAg) was adjuvanted with OG-protected frozen AlOOH NPs in mice, the loss of immunogenicity was inhibited. These findings provide insights into the freezing-induced surface decomposition of Alum and can be translated to design of protectants to improve the stability of vaccines.
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http://dx.doi.org/10.1016/j.isci.2022.104456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301878PMC
June 2022

A computational study of the HO + SO → HOSO + O reaction catalyzed by a water monomer, a water dimer and small clusters of sulfuric acid: kinetics and atmospheric implications.

Phys Chem Chem Phys 2022 Aug 3;24(30):18205-18216. Epub 2022 Aug 3.

Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.

Herein, the reaction mechanisms and kinetics for the HO + SO → HOSO + O reaction catalyzed by a water monomer, a water dimer and small clusters of sulfuric acid have been studied theoretically by quantum chemical methods and the Master Equation/Rice-Ramsperger-Kassel-Marcus (ME/RRKM) rate calculations. The calculated results show that when HO is introduced into the HO + SO reaction, it not only enhances the stability of the reactant complexes by 9.0 kcal mol but also reduces the energy of the transition state by 8.7 kcal mol. As compared with HO, catalysts (HO), HSO, HSO⋯HO and (HSO) are more effective energetically, which not only results from a higher binding energy of 21.3-26.0 kcal mol for the reactant complexes but also from a reduction of the energy of the transition states by 8.6-17.2 kcal mol. Effective rate constant calculations show that, as compared with HO, catalysts (HO), HSO, HSO⋯HO and (HSO) can never become more efficient catalysts within the altitude range of 0-15 km due to their relatively lower concentrations. Besides, at 0 km altitude, the enhancement factor for the HO and ('/) (HO)-assisted HO + SO reaction within the temperature range of 280-320 K was respectively calculated to be 0.31%-0.34% and 0.16%-0.27%, while the corresponding enhancement factor of HO and (HO) at higher altitudes of 5-15 km was respectively found only 0.002%-0.12% and 0.00001%-0.022%, indicating that the contributions of HO and (HO) are not apparent in the gas-phase reaction of HO with SO especially at higher altitude. Overall, the present work will give a new insight into how a water monomer, a water dimer and small clusters of sulfuric acid catalyze the HO + SO → HOSO + O reaction.
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http://dx.doi.org/10.1039/d1cp03318bDOI Listing
August 2022

Synthesis and biological evaluation of 4-(4-aminophenyl)-6-methylisoxazolo[3,4-b] pyridin-3-amine covalent inhibitors as potential agents for the treatment of acute myeloid leukemia.

Bioorg Med Chem 2022 Sep 16;70:116937. Epub 2022 Jul 16.

Laboratory of Molecular Design and Drug Discovery, School of Science, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, Jiangsu 211198, China. Electronic address:

Fms-like tyrosine kinase 3 (FLT3) mutation has been strongly associated with increased risk of relapse, and the irreversible covalent FLT3 inhibitors had the potential to overcome the drug-resistance. In this study, a series of simplified 4-(4-aminophenyl)-6-methylisoxazolo[3,4-b] pyridin-3-amine derivatives containing two types of Michael acceptors (vinyl sulfonamide, acrylamide) were conveniently synthesized to target FLT3 and its internal tandem duplications (ITD) mutants irreversibly. The kinase inhibitory activities showed that compound C14 displayed potent inhibition activities against FLT3 (IC = 256 nM) and FLT3-ITD by 73 % and 25.34 % respectively, at the concentration of 1 μM. The antitumor activities indicated that C14 had strong inhibitory activity against the human acute myeloid leukemia (AML) cell lines MOLM-13 (IC = 507 nM) harboring FLT3-ITD mutant, as well as MV4-11 (IC = 325 nM) bearing FLT3-ITD mutation. The biochemical analyses showed that these effects were related to the ability of C14 to inhibit FLT3 signal pathways, and C14 could induce apoptosis in MV4-11 cell as demonstrated by flow cytometry. Fortunately, C14 showed very weak potency against FLT3-independent human cervical cancer cell line HL-60 (IC > 10 μM), indicating that it might have no off-target toxic effects. In light of these data, compound C14 represents a novel covalent FLT3 kinase inhibitor for targeted therapy of AML.
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http://dx.doi.org/10.1016/j.bmc.2022.116937DOI Listing
September 2022

Discussing the Effect of Students' Crisis Awareness on Emotion During the COVID-19 Pandemic From the Perspective of Trust.

Front Psychol 2022 4;13:803372. Epub 2022 Jul 4.

Network Information Center, Nanchang Institute of Technology, Nanchang, China.

The effects of crises vary among individuals, societies, and nations. Governments' crisis management is quite different from that of non-governmental organizations, especially in terms of "publicity," since it involves bureaucracy to address people's accountability concerns. The purpose of this study is to investigate the relationship between students' crisis awareness, trust, and emotions in the event of a major public health emergency. A questionnaire survey was conducted for this study. A total of 500 copies of questionnaires were distributed to the college students in Jiangxi. Among those, 437 valid copies were retrieved, with a retrieval rate of 87%. A structural equation model (SEM) was used to conduct the statistical analyses. The research results were summarized as follows: (1) At the stage of epidemic spread, people can easily fall into the negative emotion. (2) The society with a good trust relationship considers schools less responsible for critical incidents and more helpful for crisis communication. (3) Reducing the negative emotions of the public after the occurrence of critical incidents can effectively reduce the damage of critical incidents to the organization. Avoiding a loss of student confidence and increasing anger, protecting the school's reputation, having a good communication effect, and minimizing the impact of the crisis can help the students develop better trust toward the school. When a crisis occurs on campus, this can reduce the possibility of students' showing negative emotions and spreading rumors. It is considered that the findings provide guidance on how to optimize the management of public health crisis situations and improve students' mental health.
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http://dx.doi.org/10.3389/fpsyg.2022.803372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291431PMC
July 2022

Effects of Traditional Chinese Fitness Exercises on Negative Emotions and Sleep Disorders in College Students: A Systematic Review and Meta-Analysis.

Front Psychol 2022 4;13:908041. Epub 2022 Jul 4.

School of Sports and Human Body Science, Shenyang Sport University, Shenyang, China.

Background: The purpose of this study was to systematically review the effectiveness of regular traditional Chinese fitness exercises on negative emotions and sleep disorders in college students, and to provide evidence-based evidence and new ideas for the negative emotions and sleep disorders among the college students.

Methods: A systematic search using 5 English (PubMed, Embase, Scopus, EBSCO, and the Cochrane Library) and 4 Chinese (CNKI, WanFang, VIP, and CBM) databases were initiated to identify randomized controlled trials (RCT) assessing the effect of traditional Chinese fitness exercises on negative emotions and sleep disorders among college students. Standardized mean differences (SMD) and their 95% confidence intervals () were used to determine the pooled effect of the intervention. The Cochrane bias risk assessment tool was used to evaluate the methodological quality and the data were analyzed with Review Manager 5.4.

Results: A total of 12 RCTs were included, including 1,052 subjects. The results showed a potential beneficial effect of traditional Chinese fitness exercises on reducing depression [SMD = -0.93, 95 % (-1.76, -0. 10)], anxiety [SMD = -0.74, 95% (-0.93, -0.54)], and the sleep disorders [SMD = -2.77, 95% (-4.57, -0.97)] symptoms, and these effects were better than in the control group.

Conclusion: The findings of this review suggested the traditional Chinese fitness exercises could improve both the negative moods and sleep disorders compared with that of healthy students, the effect on college students with mild to moderate psychological symptoms was obviously improved. The SCL-90 scale is better than the SDS scale in evaluating the improvement effect of traditional Chinese fitness exercises on depression. It was the best intervention program on negative emotions and sleep disorders among college students, with the intervention lasting 5 sessions per week for 30-60 min/session for over 12 weeks.
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http://dx.doi.org/10.3389/fpsyg.2022.908041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291289PMC
July 2022

A typical presentation of moxifloxacin-induced DRESS syndrome with pulmonary involvement: a case report and review of the literature.

BMC Pulm Med 2022 Jul 19;22(1):279. Epub 2022 Jul 19.

Beijing Jishuitan Hospital, Fourth Clinical College of Peking University, No. 31 Xinjiekou East Street, Xicheng District, Beijing, 100035, China.

Background: Drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome is a kind of hypersensitivity drug reaction involving the skin and multiple internal organ systems. Moxifloxacin has rarely been reported to be a drug that is associated with DRESS syndrome. Lungs are less frequently involved in DRESS syndrome, but their involvements may herald more serious clinical processes. We present a rare typical case of moxifloxacin-induced DRESS syndrome with lungs involved. Valuable clinical data such as changes in the pulmonary imaging and pulmonary function tests was recorded. This case is important for the differential diagnosis of DRESS syndrome with lungs involved by providing clinical manifestations, CT imaging, pulmonary function tests, and biopsy pathological characteristics. The changes in pulmonary imaging and pulmonary function tests may help us understand the mechanism of DRESS syndrome further.

Case Presentation: We report a case of a 47-year-old woman who was treated with oral moxifloxacin for community-acquired pneumonia. The patient subsequently developed a cough, fever, liver injury, skin rash, hematologic abnormalities, and shortness of breath (SOB) followed by pharyngeal herpes and peripheral neuritis. These symptoms, clinical lab index, and CT scan of the lungs improved after the withdrawal of moxifloxacin. The probability of moxifloxacin-induced DRESS syndrome was rated as "Definite", with 7 scores graded by RegiSCAR. A literature search was also performed with "fluoroquinolones," "moxifloxacin," "ciprofloxacin," "levofloxacin," "delafloxacin," and "DRESS" or "drug-induced hypersensitivity syndrome (DIHS)" as the keywords that were put into PubMed. The overall pulmonary involvement was approximately 9.1% (1/11). It is a rare reported case of DRESS syndrome with pulmonary involvement induced by moxifloxacin. We summarized detailed clinical data, including pulmonary imaging and pulmonary function changes.

Conclusion: This is a rare reported case of DRESS syndrome with pulmonary involvement induced by moxifloxacin. Prompt recognition and correct diagnosis can promote appropriate treatment and accelerate recovery. This case is important for us as a reference in the differential diagnosis of DRESS syndrome and helps us further understand the mechanism of DRESS syndrome.
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http://dx.doi.org/10.1186/s12890-022-02064-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297593PMC
July 2022

Self-assembled aluminum oxyhydroxide nanorices with superior suspension stability for vaccine adjuvant.

J Colloid Interface Sci 2022 Jul 11;627:238-246. Epub 2022 Jul 11.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China; School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China. Electronic address:

The suspension stability of aluminum-based adjuvant (Alum) plays an important role in determining the Alum-antigen interaction and vaccine efficacy. Inclusion of excipients has been shown to stabilize antigens in vaccine formulations. However, there is no mechanistic study to tune the characteristics of Alum for improved suspension stability. Herein, a library of self-assembled rice-shaped aluminum oxyhydroxide nanoadjuvants i.e., nanorices (NRs), was synthesized through intrinsically controlled crystallization and atomic coupling-mediated aggregations. The NRs exhibited superior suspension stability in both water and a saline buffer. After adsorbing hepatitis B surface antigen (HBsAg) virus-like particles (VLPs), human papillomavirus virus (HPV) VLPs, or bovine serum albumin, NR-antigen complexes exhibited less sedimentation. Further mechanistic study demonstrated that the improved suspension stability was due to intraparticle aggregations that led to the reduction of the surface free energy. By using HBsAg in a murine vaccination model, NRs with higher aspect ratios elicited more potent humoral immune responses. Our study demonstrated that engineered control of particle aggregation provides a novel material design strategy to improve suspension stability for a diversity of biomedical applications.
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http://dx.doi.org/10.1016/j.jcis.2022.07.022DOI Listing
July 2022

3-Bromopyruvic acid regulates glucose metabolism by targeting the c-Myc/TXNIP axis and induces mitochondria-mediated apoptosis in TNBC cells.

Exp Ther Med 2022 Aug 16;24(2):520. Epub 2022 Jun 16.

Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Aerobic glycolysis is commonly observed in tumor cells, including triple-negative breast cancer (TNBC) cells, and the rate of aerobic glycolysis is higher in TNBC cells than in non-TNBC cells. Hexokinase 2 (HK2) is a key enzyme in the glycolytic pathway and a target of the transcription factor c-Myc, which is highly expressed in TNBC and promotes aerobic glycolysis by enhancing HK2 expression. As an inhibitor of HK2, 3-bromopyruvic acid (3-BrPA) exhibits good therapeutic efficacy in intrahepatic and extrahepatic tumors and inhibits the proliferation of human tumor cells with high expression levels of c-Myc and . In addition, 3-BrPA combines with photodynamic therapy to inhibit TNBC cell migration. Thioredoxin-interacting protein (TXNIP) competes with c-Myc to reduce glucose consumption in tumor cells to restrain cell proliferation. A comparative analysis was performed in the present study in TNBC (HCC1143) and non-TNBC (MCF-7) cell lines to explore the effect of 3-BrPA on energy metabolism in TNBC cells and to investigate the possible mechanism of action. Cell viability and apoptosis were detected through Cell Counting Kit-8 and flow cytometry assays, respectively. Expression levels of HK2, glucose transporter 1, TXNIP, c-Myc and mitochondria-regulated apoptosis pathway proteins were measured through western blotting. 3-BrPA inhibited cell proliferation, downregulated c-Myc and HK2 expression, and upregulated TXNIP expression in TNBC cells, but it doesn't have the same effect on non-TNBC cells. Furthermore, 3-BrPA induced the typical manifestations of mitochondrial-mediated apoptosis such as decreasing Bcl-2 expression and increasing Bax, Cyt-C and Caspase-3 expression. The present results suggested that 3-BrPA promoted TXNIP protein expression and reduced HK2 expression in TNBC cells by downregulating c-Myc expression, inhibiting glycolysis including suppressing lactate generation, intracellular ATP generation and HK activity, inducing mitochondrial-mediated apoptosis and eventually suppressing TNBC cell proliferation. These findings may reveal a novel therapeutic target for the clinical treatment of TNBC.
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http://dx.doi.org/10.3892/etm.2022.11447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257941PMC
August 2022

Essential role of IL-17 in acute exacerbation of pulmonary fibrosis induced by non-typeable .

Theranostics 2022 4;12(11):5125-5137. Epub 2022 Jul 4.

Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia 19104, USA.

Acute exacerbation (AE) of idiopathic pulmonary fibrosis (IPF) has a poor prognosis and lacks effective therapy. Animal models that mimic AE-IPF can greatly accelerate investigation of its pathogenesis and development of effective therapy. However, there are few reports of animal models of AE-IPF caused by bacteria. Thus, our study aimed to establish a mouse model of bacterium-induced AE-IPF and explore the potential pathogenic mechanism of AE-IPF. Mice were instilled intranasally with bleomycin (BLM) followed by non-typeable (NTHi) strain NT127. Murine survival, bacterial load, body weight and pulmonary histopathological changes were evaluated. We analyzed the T cell and inflammatory cell responses in the lungs. Infection with 10 CFU NT127 triggered AE in mice with PF induced by 30 μg BLM. Compared with BLM-instilled mice, the BLM/NT127-treated mice showed more obvious airway inflammation, lower survival rate, higher inflammatory cell response, and increased proportions and numbers of IL-17CD4, IL-17 γδ T, IL-22CD4 and regulatory T (Treg) cells in lungs. γδ T cells were the predominant source of IL-17. IL-17 gene knockout mice with AE-IPF had quicker body weight recovery, milder pulmonary inflammation and fibrosis, stronger IL-22CD4T, TGF-β γδ T and Treg cell responses, and weaker neutrophil and eosinophil responses than wild-type mice with AE-IPF. NTHi infection after BLM-induced IPF can cause AE-IPF in a murine model. This novel model can be used to investigate the pathogenesis of AE-IPF and develop new therapies for AE-IPF caused by bacteria. IL-17 is essential for the development of AE-IPF, and it may be a new therapeutic target for bacteria-induced AE-IPF.
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http://dx.doi.org/10.7150/thno.74809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274745PMC
July 2022

A Wadsley-Roth crystallographic shear phase SrNbO anode for fast Li-ion storage.

Chem Commun (Camb) 2022 Aug 2;58(62):8626-8629. Epub 2022 Aug 2.

Institute of Materials Research, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.

SrNbO, featuring an enlarged unit-cell volume (Wadsley-Roth phase), is selected as a model anode to investigate its fast-charging behaviors. This novel SrNbO exhibits a large reversible capacity of 223.4 mA h g at 0.3C, high rate performance (20C) and remarkable cyclability (capacity retention rate of 80.5% after 850 cycles).
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http://dx.doi.org/10.1039/d2cc03208bDOI Listing
August 2022

De novo assembly of human genome at single-cell levels.

Nucleic Acids Res 2022 07;50(13):7479-7492

School of Life Sciences, Biomedical Pioneering Innovation Center, Peking University, Beijing 100871, China.

Genome assembly has been benefited from long-read sequencing technologies with higher accuracy and higher continuity. However, most human genome assembly require large amount of DNAs from homogeneous cell lines without keeping cell heterogeneities, since cell heterogeneity could profoundly affect haplotype assembly results. Herein, using single-cell genome long-read sequencing technology (SMOOTH-seq), we have sequenced K562 and HG002 cells on PacBio HiFi and Oxford Nanopore Technologies (ONT) platforms and conducted de novo genome assembly. For the first time, we have completed the human genome assembly with high continuity (with NG50 of ∼2 Mb using 95 individual K562 cells) at single-cell levels, and explored the impact of different assemblers and sequencing strategies on genome assembly. With sequencing data from 30 diploid individual HG002 cells of relatively high genome coverage (average coverage ∼41.7%) on ONT platform, the NG50 can reach over 1.3 Mb. Furthermore, with the assembled genome from K562 single-cell dataset, more complete and accurate set of insertion events and complex structural variations could be identified. This study opened a new chapter on the practice of single-cell genome de novo assembly.
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http://dx.doi.org/10.1093/nar/gkac586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303314PMC
July 2022

Low molecular-weight polyguluronate phosphate: An immunostimulant by activating splenocyte/macrophage invitro and improving immune response invivo.

Int J Biol Macromol 2022 Jul 6;216:510-519. Epub 2022 Jul 6.

Shandong Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Laboratory of Marine Glycodrug Research and Development, Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China. Electronic address:

The substituents and backbones are two main factors affecting immune activities of polysaccharides. In the present study, we firstly evaluated the immunostimulating effects of phosphorylated, sulfated, H-phosphonated and nitrated derivatives of low-molecular-weight polymannuronate (LPM) and polyguluronate (LPG) on splenocytes and peritoneal macrophages in vitro. The results showed that the phosphate group was the best substituent to enhance the immune activities, and LPG phosphate (LPGP) had much better activity than LPM phosphate (LPMP). Further studies showed that LPGP not only promoted the proliferation of mouse splenocytes in the presence of either LPS or Con A, but also acted as an excellent peritoneal macrophage activator to enhance the cell phagocytosis, energy metabolism, cytokines release and activities of intracellular enzymes. The studies in RAW264.7 cells revealed that LPGP activated the TBK1-IκBα-NF-κB and the TBK1-IRF3 pathway. Moreover, LPGP rescued the immune response in the Cyclophosphamide-treated mice in vivo. In conclusion, LPGP is a potential alginate-based biological response modifier (BRM).
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.001DOI Listing
July 2022

Nomogram to predict risk and prognosis of synchronous lung metastasis in renal cell carcinoma: A large cohort analysis.

Medicine (Baltimore) 2022 Jul 8;101(27):e29764. Epub 2022 Jul 8.

School of Electronic Engineering, Chao Hu University, Chaohu, China.

We aimed to construct and validate nomogram models that predict the incidence of lung metastasis (LM) in patients with renal cell carcinoma (RCC) and evaluate overall survival (OS) and cancer-specific survival (CSS) among RCC patients with LM. The Surveillance, Epidemiology, and End Results database was analyzed for RCC patients diagnosed between 2010 and 2015. The X-tile program was used to determine the best cutoff values for age at initial diagnosis and tumor size. Logistic regression analysis was performed to explore independent risk factors for LM, and COX regression analysis was used to identify prognostic indicators for OS and CSS in lung metastatic RCC patients. Subsequently, 3 nomograms were established, and receiver operating characteristic (ROC) curves and decision curve analysis (DCA) were utilized to validate their accuracy. We randomly assigned 10,929 patients with RCC to 2 groups with 1:1 allocation. Multivariate logistic analyses revealed that pathology, tumor (T) stage, nodes (N) stage, race, grade, surgery, metastatic sites, and tumor size were independent risk factors for LM. Multivariate Cox analyses showed that pathology, T stage, N stage, age, surgery, metastatic sites, and residence were independent prognostic factors for OS and CSS in patients with LM. Then, nomograms were developed based on the multivariate logistic and Cox regression analyses results. The ROC and DCA curves confirmed that these nomograms achieved satisfactory discriminative power. Three effective nomograms were constructed and validated that can be used to assist clinicians in predicting the incidence of LM and evaluating the prognosis of lung metastatic RCC.
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http://dx.doi.org/10.1097/MD.0000000000029764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259154PMC
July 2022

Vitexin Mitigates -Induced Mastitis via Regulation of ROS/ER Stress/NF-B/MAPK Pathway.

Oxid Med Cell Longev 2022 27;2022:7977433. Epub 2022 Jun 27.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

Mastitis, caused by a variety of pathogenic microorganisms, seriously threatens the safety and economic benefits of the dairy industry. Vitexin, a flavone glucoside found in many plant species, has been widely reported to have antioxidant, anti-inflammatory, antiviral, anticancer, neuroprotective, and cardioprotective effects. However, few studies have explored the effect of vitexin on mastitis. This study is aimed at exploring whether the antioxidant and anti-inflammatory functions of vitexin can improve Staphylococcus aureus-induced mastitis and its possible molecular mechanism. The expression profiles of S. aureus-infected bovine mammary epithelial cells and gland tissues from the GEO data set (GSE94056 and GSE139612) were analyzed and found that DEGs were mainly involved in immune signaling pathways, apoptosis, and ER stress through GO and KEGG enrichment. Vitexin blocked the production of ROS and increased the activity of antioxidant enzymes (SOD, GSH-PX, and CAT) via activation of PPAR and . In addition, vitexin reduced the production of inflammatory cytokines (TNF-, IL-1, and IL-6) and inhibited apoptosis in MAC-T cells and mouse mammary tissues infected with Staphylococcus aureus. Moreover, vitexin decreased the expression of PDI, Ero1-L, p-IRE1, PERK, p-eIF2, and CHOP protein but increased BiP in both mammary gland cells and tissues challenged by . Western blot results also found that the phosphorylation levels of JNK, ERK, p38, and p65 were reduced in vitexin-treated tissues and cells. Vitexin inhibited the production of ROS through promoting PPAR, increased the activity of antioxidant enzymes, and reduced inflammatory cytokines and apoptosis by alleviating ER stress and inactivation MAPKs and NF-B signaling pathway. Vitexin maybe have great potential to be a preventive and therapeutic agent for mastitis.
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http://dx.doi.org/10.1155/2022/7977433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252844PMC
July 2022

Alienation Control Technology of Information Pushed by Intelligent Algorithms in Media Communication.

Comput Intell Neurosci 2022 27;2022:4855254. Epub 2022 Jun 27.

School of Journalism and Communication, Wuhan University, Wuhan 430072, Hubei, China.

"Information alienation" is a concept formed by combining the idea of "alienation" in philosophy with the characteristics of information itself. Information is a universal property of matter and an objectively existing form of material movement. The purpose of this paper is to study and analyze the alienation technology control of push information based on intelligent algorithms and describe the information alienation. This paper puts forward the problem of pushing information, which is based on intelligent algorithm, then elaborates around the concept of intelligent algorithm and related algorithms, and designs and analyzes the control of media information alienation. The experimental results show that, in the questionnaire survey, 72.34% of the people who very frequently swipe unfriendly information account for a very large proportion. People will always swipe it in various software applications, which is unavoidable. How to control the problem of information alienation needs to be controlled from the user level, the design media level, and the government level.
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http://dx.doi.org/10.1155/2022/4855254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252669PMC
July 2022

Distribution of sediment microbial communities and their relationship with surrounding environmental factors in a typical rural river, Southwest China.

Environ Sci Pollut Res Int 2022 Jul 2. Epub 2022 Jul 2.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, China.

With rapid urbanization and industrialization, rural rivers in China are facing deterioration in water quality and ecosystem health. Microorganisms living in river sediments are involved in biogeochemical processes, mineralization, and degradation of pollutants. Understanding bacterial community distribution in rural rivers could help evaluate the response of river ecosystems to environmental pollution and understand the river self-purification mechanism. In this study, the relationship between characteristics of sediment microbial communities and the surrounding environmental factors in a typical rural river was analyzed using 16S rRNA gene sequencing technology. The results showed that the dominant bacterial groups in the river sediment were Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, Bacteroidetes, and Firmicutes, accounting for 83.61% of the total microbial load. Different areas have different sources of pollution which give rise to specific dominant bacteria. The upstream part of the river flows through an agricultural cultivation area where the dominant bacteria were norank_f_Gemmatimonadaceae, Haliangium, and Pseudolabrys, possessing obvious nitrogen- and phosphorus-metabolizing activities. The midstream section flows through an urban area where the dominant bacteria were Marmoricola, Nocardioides, Gaiella, Sphingomonas, norank_f_67-14, Subgroup_10, Agromyces, and Lysobacter, with strong metabolizing activity for toxic pollutants. The dominant bacteria in the downstream part were Clostridium_sensu_stricto_1, norank_f__Bacteroidetes_vadinHA17, Candidatus_Competibacter, and Methylocystis. Redundancy analysis and correlation heatmap analysis showed that environmental factors: ammonia nitrogen (NH-N) and total nitrogen (TN) in the sediment, and pH, temperature, TN, electrical conductivity (EC), and total dissolved solids (TDS) in the water, significantly affected the bacterial community in the sediment. The PICRUSt2 functional prediction analysis identified that the main function of bacteria in the sediment was metabolism (77.3%), specifically carbohydrate, amino acid, and energy metabolism. These activities are important for degrading organic matter and removing pollutants from the sediments. The study revealed the influence of organic pollutants derived from human activities on the bacterial community composition in the river sediments. It gave a new insight into the relationship between environmental factors and bacterial community distribution in rural watershed ecosystems, providing a theoretical basis for self-purification and bioremediation of rural rivers.
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http://dx.doi.org/10.1007/s11356-022-21627-7DOI Listing
July 2022

Quantifying Spin-Orbit Torques in Antiferromagnet-Heavy-Metal Heterostructures.

Phys Rev Lett 2022 Jun;128(24):247204

Center for Quantum Phenomena, Department of Physics, New York University, New York 10003, USA.

The effect of spin currents on the magnetic order of insulating antiferromagnets (AFMs) is of fundamental interest and can enable new applications. Toward this goal, characterizing the spin-orbit torques (SOTs) associated with AFM-heavy-metal (HM) interfaces is important. Here we report the full angular dependence of the harmonic Hall voltages in a predominantly easy-plane AFM, epitaxial c-axis oriented α-Fe_{2}O_{3} films, with an interface to Pt. By modeling the harmonic Hall signals together with the α-Fe_{2}O_{3} magnetic parameters, we determine the amplitudes of fieldlike and dampinglike SOTs. Out-of-plane field scans are shown to be essential to determining the dampinglike component of the torques. In contrast to ferromagnetic-heavy-metal heterostructures, our results demonstrate that the fieldlike torques are significantly larger than the dampinglike torques, which we correlate with the presence of a large imaginary component of the interface spin-mixing conductance. Our work demonstrates a direct way of characterizing SOTs in AFM-HM heterostructures.
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http://dx.doi.org/10.1103/PhysRevLett.128.247204DOI Listing
June 2022

Surgical Treatment of Vertical Shear Pelvic Fracture Associated with a Lumbosacral Plexus Injury through the Lateral-Rectus Approach: Surgical Techniques and Preliminary Outcomes.

Orthop Surg 2022 Aug 30;14(8):1723-1729. Epub 2022 Jun 30.

Department of Traumatic Surgery, Center for Orthopaedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

Objective: To examine the surgical techniques and preliminary outcomes of the lateral rectus approach (LRA) for treating vertical shear (VS) pelvic fracture associated with lumbosacral plexus (LSP) injury.

Methods: This study was a retrospective trial. From August 2010 to October 2017, 29 patients with VS pelvic fractures involving LSP injury who were treated with the LRA were included in this study. The patients were 18-61 years old, with a mean age of 36.2 years. All patients underwent neurolysis, open reduction, and internal fixation (ORIF) through the LRA. The fracture reduction was evaluated using the Matta criteria, and the neural recovery was evaluated by muscle strength grading proposed by the British Medical Research Council (BMRC).

Results: All 29 patients underwent the surgery successfully. The mean operating time was 155.2 ± 32.1 min (range: 105-220 min). The mean operative blood loss was 1021.4 ± 363.4 mL (range: 400-2000 mL). All patients were followed-up for at least 24 months (mean, 32.8 ± 13.5 months; range: 24-96 months). According to the Matta criteria, there were 17 excellent cases, nine good cases, and three fair cases in 29 patients. The ratio of excellent-to-good cases was 89.66%. According to the criteria of the Nerve Injuries Committee of the British Medical Research Council (BMRC), the recovery of nerve and muscle strength achieved to M5 (full recovery of neurological symptoms) was 14 cases, M4 (fine recovery of neurological symptoms), seven cases; M1, M2, and M3 (partial recovery of neurological symptoms), five cases, and M0 (no recovery of neurological symptoms), three cases.

Conclusions: LRA is a safe and feasible surgical approach for treating VS pelvic fractures with LSP injury, which can be used to perform nerve exploration and release from the front, reduce the fracture, and fix it with the anterior iliac plates and/or sacroiliac screws.
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http://dx.doi.org/10.1111/os.13359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363718PMC
August 2022

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of mA-modified CREB1 mRNA.

Mol Cancer 2022 06 30;21(1):140. Epub 2022 Jun 30.

Departments of Gastroenterology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Department of Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu Province, China.

Background: Aberrant expression of circular RNAs (circRNAs) contributes to the initiation and progression of human malignancies, but the underlying mechanisms remain largely elusive.

Methods: High-throughput sequencing was performed to screen aberrantly expressed circRNAs or miRNAs in colorectal cancer (CRC) and adjacent normal tissues. A series of gain- and loss-of-function studies were conducted to evaluate the biological behaviors of CRC cells. RNA pulldown, mass spectrometry, RIP, qRT-PCR, Western blot, luciferase reporter assays and MeRIP-seq analysis were further applied to dissect the detailed mechanisms.

Results: Here, a novel circRNA named circEZH2 (hsa_circ_0006357) was screened out by RNA-seq in CRC tissues, whose expression is closely related to the clinicpathological characteristics and prognosis of CRC patients. Biologically, circEZH2 facilitates the proliferation and migration of CRC cells in vitro and in vivo. Mechanistically, circEZH2 interacts with mA reader IGF2BP2 and blocks its ubiquitination-dependent degradation. Meanwhile, circEZH2 could serve as a sponge of miR-133b, resulting in the upregulation of IGF2BP2. Particularly, circEZH2/IGF2BP2 enhances the stability of CREB1 mRNA, thus aggravating CRC progression.

Conclusions: Our findings not only reveal the pivotal roles of circEZH2 in modulating CRC progression, but also advocate for attenuating circEZH2/miR-133b/IGF2BP2/ CREB1 regulatory axis to combat CRC.
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http://dx.doi.org/10.1186/s12943-022-01608-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245290PMC
June 2022

Protein PvTRAg23 Triggers Spleen Fibroblasts for Inflammatory Profile and Reduces Type I Collagen Secretion NF-κBp65 Pathway.

Front Immunol 2022 13;13:877122. Epub 2022 Jun 13.

Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China.

is the most widespread human malaria parasite. The spleen is one of the most significant immune organs in the course of infection, and it contains splenic fibroblasts (SFs), which supports immunologic function by secreting type I collagen (collagen I). proteins have rarely been found to be involved in collagen alterations in the spleen during infection. Here, we selected the protein tryptophan-rich antigen 23 (PvTRAg23), which is expressed by the spleen-dependent gene and is a member of the PvTRAgs family of export proteins, suggesting that it might have an effect on SFs. The protein specifically reduced the level of collagen I in human splenic fibroblasts (HSFs) and bound to cells with vimentin as receptors. However, such collagen changes were not mediated by binding to vimentin, but rather activating the NF-κBp65 pathway to produce inflammatory cytokines. Collagen impaired synthesis accompanied by extracellular matrix-related changes occurred in the spleen of mice infected with 17XNL. Overall, this study is the first one to report and verify the role of proteins on collagen in HSF . Results will contribute to further understanding of host spleen structural changes and immune responses after infection.
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http://dx.doi.org/10.3389/fimmu.2022.877122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235351PMC
June 2022

Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESC.

Stem Cell Res Ther 2022 06 28;13(1):282. Epub 2022 Jun 28.

Laboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, Higher Education Mega Center, South China University of Technology, No.382 Waihuan East Road, Suite 406, Guangzhou, 510006, People's Republic of China.

Background: Hematopoietic stem cells are able to self-renew and differentiate into all blood cell lineages. Hematopoietic stem cell transplantation is a mainstay of life-saving therapy for hematopoietic malignancies and hypoproliferative disorders. In vitro hematopoietic differentiation of human pluripotent stem cells (hPSCs) is a promising approach for modeling hematopoietic development and cell replacement therapies. Although using hPSCs to derive hematopoietic progenitor cells has achieved some successes in the past, differentiation from hPSCs to produce all hematopoietic cells which can provide robust long-term multilineage engraftment is still very difficult. Here, we reported a novel culture system for hematopoietic differentiation from human embryonic stem cells (hESCs) with optimal cytokines combinations under hypoxia condition.

Methods: In vitro production of T lineage hematopoietic stem/progenitor cells from hESCs by using hypoxia differentiation system, the effects and the potential mechanism of hypoxia promoting T lineage hematopoiesis were investigated by RT-qPCR validation, cell cycle assay and flow cytometry analysis.

Results: Using our differentiation system, almost 80% CD45 cells generated from hESCs were hematopoietic cells and particularly could be further induced into CD3TCRαβ T cells in vitro. We detected more CD34CD144 hematopoietic endothelial progenitors (HEPs) induced from hESCs than those in normoxia conditions, and the early HEPs-related gene DLL4 was upregulated by enhancing the hypoxia signaling via potential HIF-1α/NOTCH1/DLL4 axis to enhance arterial feature, thus drove T lineage during the hematopoiesis. Strikingly, hematopoietic cells generated in our system exhibited the potential for all multilineage reconstruction including lymphoid, myeloid and erythroid lineages in vivo by transplantation assay.

Conclusion: Our results demonstrated that hypoxia plays an important role in T lineage hematopoiesis by promoting the expression of arterial endothelial gene DLL4 and upregulation of NOTCH1 through the activation of the HIF-1α signaling pathway. These results provide a significant approach for in vitro and in vivo production of fully functional hematopoietic stem/progenitor cells from hESCs.
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http://dx.doi.org/10.1186/s13287-022-02967-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241298PMC
June 2022
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