Publications by authors named "Cheng Wang"

2,309 Publications

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Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a.

Cell Death Differ 2021 May 14. Epub 2021 May 14.

Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China.

Accumulating evidence has shown that long noncoding RNAs (lncRNAs) are involved in several biological processes, including immune responses. However, the role of lncRNAs in antiviral innate immune responses remains largely elusive. Here, we identify an uncharacterized human lncRNA AVAN from influenza A virus (IAV) infected patients, that is significantly upregulated following RNA virus infection. During IAV infection, AVAN play an indispensable role in antiviral immune responses. In vivo, we enforced the expression of AVAN in transgenic mice or adeno-associated virus encoding AVAN delivery system and found that AVAN significantly alleviated IAV virulence and virus replication. Mechanistically, nuclear AVAN positively regulates the transcription of forkhead box O3A (FOXO3a) by associating with its promoter and inducing chromatin remodeling to promote neutrophil chemotaxis. Meanwhile, cytoplasmic AVAN binds directly to the E3 ligase TRIM25 and enhances TRIM25-mediated K63-linked ubiquitination of RIG-I, thereby promoting TRIM25- and RIG-I-mediated antiviral innate immune responses, including the induction of type I interferon and ISGs. Moreover, AVAN binds to the B Box/CCD domain of TRIM25 and 1-200nt of AVAN were the functional moieties. Collectively, our findings highlight the potential clinical implications of human lncRNA AVAN as a key positive regulator of the antiviral innate immune response and a promising target for developing broad antiviral therapeutics.
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http://dx.doi.org/10.1038/s41418-021-00791-2DOI Listing
May 2021

Supplier selection mechanism in electric coal procurement under sustainability.

Environ Sci Pollut Res Int 2021 May 14. Epub 2021 May 14.

School of Science, Wuhan University of Technology, Wuhan, 430070, People's Republic of China.

The electric energy mainly comes from thermal power generation fueled by electric coal, and the selection of electric coal supplier is of great practical significance for the development of electric power enterprises. In this paper, the mechanism of supplier selection in electric coal procurement is designed from a perspective of sustainability. Concretely, eleven evaluation indexes from three aspects of economy, environment, and society are selected to construct a new evaluation index system for selecting sustainable electric coal suppliers. Based on this index system, a new method of supplier selection in electric coal procurement based on 2-tuple correlation coefficient analysis is proposed. In this method, considering that the evaluation index values have the features of multi-source heterogeneous data, i.e., precise real number, interval number, and fuzzy linguistic variables coexist, all index values are converted into 2-tuples. Then, a 2-tuple deviation maximization method is proposed to determine the weight of each evaluation index, and a new method of 2-tuple correlation coefficient analysis is proposed to select sustainable electric coal suppliers. Moreover, the effectiveness and feasibility of the model are highlighted by an application example of selecting the electric coal suppliers. Combined with the development status of electric power industry and the research results of this paper, several countermeasures and suggestions are provided on how to formulate the optimal procurement strategy of electric coal for electric power enterprises and how to improve the competitiveness for electric coal suppliers.
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http://dx.doi.org/10.1007/s11356-021-14071-6DOI Listing
May 2021

Assessment of HCMV-encoded microRNAs in plasma as potential biomarkers in pregnant women with adverse pregnancy outcomes.

Ann Transl Med 2021 Apr;9(8):638

State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China.

Background: Human cytomegalovirus (HCMV) is the most frequent cause of congenital infections and can lead to adverse pregnancy outcomes (APOs). HCMV encodes multiple microRNAs (miRNAs) that have been reported to be partially related to host immune responses, cell cycle regulation, viral replication, and viral latency, and can be detected in human plasma. However, the relevance for HCMV-encoded miRNAs in maternal plasma as an indicator for APOs has never been evaluated.

Methods: Expression profiles of 22 HCMV-encoded miRNAs were first measured in plasma samples from 20 pregnant women with APOs and 28 normal controls using quantitative reverse-transcription polymerase chain reaction. Next, markedly changed miRNAs were validated in another independent validation set consisting of 20 pregnant women with APOs and 27 control subjects. Markedly changed miRNAs were further assessed in the placenta tissues. HCMV DNA in peripheral blood leukocytes (PBLs) and anti-HCMV immunoglobulin M (IgM) and anti-HCMV immunoglobulin G (IgG) in plasma were also examined in both training and validation sets. Diagnostic value and risk factors were compared between APO cohorts and normal controls.

Results: Analysis of the training and validation data sets revealed that plasma concentrations of hcmv-miR-UL148D, hcmv-miR-US25-1-5p and hcmv-miR-US5-1 were significantly increased in pregnant women with APOs compared with normal controls. Hcmv-miR-US25-1-5p presented the largest area under the receiver-operating characteristic (ROC) curve (AUC) (0.735; 95% CI, 0.635-0.836), with a sensitivity of 68% and specificity of 71%. Furthermore, plasma levels of hcmv-miR-US25-1-5p and hcmv-miR-US5-1 correlated positively with APOs (P=0.029 and 0.035, respectively). Hcmv-miR-US25-1-5p in the placenta tissues were dramatically increased in APOs, and correlated with plasma hcmv-miR-US25-1-5p. Nevertheless, neither the concentration of HCMV DNA in PBLs nor the positivity rates of anti-HCMV IgM and anti-HCMV IgG in plasma showed a statistically significant correlation with APOs.

Conclusions: We identified a unique signature of HCMV-encoded miRNAs in pregnant women with APOs that may be useful as a potential noninvasive biomarker for predicting and monitoring APOs during HCMV infection.
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http://dx.doi.org/10.21037/atm-20-7354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106018PMC
April 2021

Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells.

STAR Protoc 2021 Jun 18;2(2):100484. Epub 2021 Apr 18.

Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.

BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the Cre-loxP recombination strategy, which allows us to study the regulation and targeting of BMI1 CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020).
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http://dx.doi.org/10.1016/j.xpro.2021.100484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081985PMC
June 2021

N6-methyladenosine demethyltransferase FTO-mediated autophagy in malignant development of oral squamous cell carcinoma.

Oncogene 2021 May 10. Epub 2021 May 10.

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.

N6-methyladenosine (mA) is the most abundant internal mRNA modification in eukaryotes and plays an important role in tumorigenesis. However, the underlying mechanism remains largely unclear. Here, we established a cell model of rapamycin-induced autophagy to screen mA-modifying enzymes. We found that mA demethylase fat mass and obesity-associated protein (FTO) plays a key role in regulating autophagy and tumorigenesis by targeting the gene encoding eukaryotic translation initiation factor gamma 1 (eIF4G1) in oral squamous cell carcinoma (OSCC). Knocked down of FTO expression in OSCC cell lines, resulting in downregulation of eIF4G1 along with enhanced autophagic flux and inhibition of tumorigenesis. Rapamycin inhibited FTO activity, and directly targeted eIF4G1 transcripts and mediated their expression in an mA-dependent manner. Dual-luciferase reporter and mutagenesis assays confirmed that YTH N6-methyladenosine RNA-binding protein 2 (YTHDF2) targets eIF4G1. Conclusively, after FTO silencing, YTHDF2 captured eIF4G1 transcripts containing mA, resulting in mRNA degradation and decreased expression of eIF4G1 protein, thereby promoting autophagy and reducing tumor occurrence. Therefore, rapamycin may regulate mA levels, determining the autophagic flux of OSCC, thereby affecting the biological characteristics of cancer cells. This insight expands our understanding of the crosstalk between autophagy and RNA methylation in tumorigenesis, which is essential for therapeutic strategy development for OSCC.
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http://dx.doi.org/10.1038/s41388-021-01820-7DOI Listing
May 2021

FUNDC1-dependent mitochondria-associated endoplasmic reticulum membranes are involved in angiogenesis and neoangiogenesis.

Nat Commun 2021 May 10;12(1):2616. Epub 2021 May 10.

Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

FUN14 domain-containing protein 1 (FUNDC1) is an integral mitochondrial outer-membrane protein, and mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs). This study aims to determine the contributions of FUNDC1-mediated MAMs to angiogenesis in vitro and in vivo. In cultured endothelial cells, VEGF significantly increases the formation of MAMs and MAM-related proteins, including FUNDC1. Endothelial cell-specific deletion of FUNDC1, which disrupts MAM formation in endothelial cells, lowers VEGFR2 expression and reduces tube formation, spheroid-sprouting, and functional blood vessel formation in vitro and in vivo. Conversely, increased MAM formation using MAM linkers mimics the effects of VEGF and promotes endothelial angiogenesis. Mechanistically, increased MAMs formation led to increased levels of Ca in cytosol, promoted the phosphorylation of serum response factor (SRF) and enhanced the binding of SRF to VEGFR2 promoter, resulting in increased VEGFR2 production, with consequent angiogenesis. Moreover, blocking FUNDC1-related MAM formation with a cell-penetrating inhibitory peptide significantly suppresses the expressions of downstream angiogenic genes and inhibits tumor angiogenesis. We conclude that decreased MAMs formation by silencing FUNDC1 can inhibit angiogenesis by decreasing VEGFR2 expression, and targeting FUNDC1-dependent MAMs might be a promising approach for treating human disorders characterized by defective angiogenesis.
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http://dx.doi.org/10.1038/s41467-021-22771-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110587PMC
May 2021

Progress in the genetic studies of spermatogenesis abnormalities.

Yi Chuan 2021 May;43(5):473-486

State Key Laboratory of Reproductive Medicine, Nanjing Medical University. Nanjing 211166, China.

About 15% couples suffer from infertility, half of which are caused by male factors. Male infertility usually manifests as teratozoospermia, oligospermia and/or asthenospermia, of which the most severe form is azoospermia. In this review, we summarize the recent progress in the study of genetic factors involved in nonobstructive azoospermia and teratozoospermia, Recently, with the rapid development of high-throughput chips and sequencing technologies, many genetic factors of spermatogenesis have been discovered and analyzed. For the nonobstructive azoospermia, genome-wide association studies (GWAS) and high-throughput sequencing revealed many risk loci of nonobstructive azoospermia. For the teratozoospermia, the application of whole-exome sequencing (WES) revealed a series of disease-causing genes, greatly enriching our knowledge of teratozoospermia including multiple morphological abnormalities of the flagella (MMAF). The discovery of lots of disease genes helped the characterization of the pathological mechanisms of male infertility. Therefore, a comprehensive and in-depth understanding of genetic factors in spermatogenesis abnormalities will play important roles in the clinical diagnosis, treatment and genetic counseling of male infertility.
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http://dx.doi.org/10.16288/j.yczz.20-343DOI Listing
May 2021

Mutations in RNA Methyltransferase Gene Confer High Risk of Outflow Tract Malformation.

Front Cell Dev Biol 2021 21;9:623394. Epub 2021 Apr 21.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

, encoding a cytosine-5 RNA methyltransferase and located in the 7q11.23 locus, is a candidate gene for tetralogy of Fallot (TOF). Deletion of the 7q11.23 locus in humans is linked to cardiac outflow tract (OFT) disorders including TOF. We identified four potential pathogenic mutations in the coding region of and which were enriched in TOF patients by an association study of 132 TOF patients and 2,000 in-house controls ( = 1.44 × 10). We then generated a null () mouse model to validate the human findings by defining the functions of in OFT morphogenesis. The OFT did not develop properly in the deletion embryonic heart. We found a misalignment of the aorta and septum defects caused by the delayed fusion of the membraneous ventricular spetum as an OFT development delay. This caused OFT development delay in 27 of 64 (42.2%) mice. Moreover, we also found OFT development delay in 8 of 51 (15.7%) mice. Further functional experiments showed that the loss of function impaired the 5-methylcytosine (mC) modification and translation efficiency of essential cardiac genes. is required for normal OFT morphogenesis and it regulates the mC modification of essential cardiac genes. Our findings suggest the involvement of in the pathogenesis of TOF.
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http://dx.doi.org/10.3389/fcell.2021.623394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097101PMC
April 2021

Digital Microfluidics: Magnetic Transportation and Coalescence of Sessile Droplets on Hydrophobic Surfaces.

Langmuir 2021 May 7. Epub 2021 May 7.

Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, 400 W. 13th Street, Rolla, Missouri 65409, United States.

Magnetic digital microfluidics is advantageous over other existing droplet manipulation methods, which exploits magnetic forces for actuation and offers the flexibility of implementation in resource-limited point-of-care applications. This article discusses the dynamic behavior of a pair of sessile droplets on a hydrophobic surface under the presence of a permanent magnetic field. A phase field method-based solver is employed in a two-dimensional computational domain to numerically capture the dynamic evolution of the droplet interfaces, which again simultaneously solves the magnetic and flow fields. On a superhydrophobic surface (i.e., θ = 150°), the nonuniform magnetic field forces the pair of sessile droplets to move toward each other, which eventually leads to a jumping off phenomenon of the merged droplet from the solid surface after coalescence. Also, there exists a critical magnetic Bond number , beyond which no coalescence event between droplets is observed. Moreover, on a less hydrophobic surface (θ ≤ 120°), the droplets still coalesce under a magnetic field, although the merged droplet does not experience any upward flight after coalescence. Also, the merging phenomenon at lower contact angle values (i.e., θ = 90°) appears significantly different than at higher contact angle values (i.e., θ = 120°). Additionally, if the pair of sessile droplets is dispersed to a different surrounding medium, the viscosity ratio plays a significant role in the upward flight of the merged droplet, where the coalesced droplet exhibits increased vertical migration at higher viscosity ratios.
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http://dx.doi.org/10.1021/acs.langmuir.1c00141DOI Listing
May 2021

Two-photon induced polymerization in a porous polymer film to create multi-layer structures.

Chem Commun (Camb) 2021 May;57(37):4516-4519

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P. R. China.

Two-photon induced polymerization for three-dimensional (3D) printing has attracted increasing attention. Here, we report the two-photon induced polymerization of triethylene glycol divinyl ether (TEGDVE) in a porous polymer film using 4,4',4''-nitrilotribenzoic acid (NTB) as the photosensitizer and diphenyliodonium hexafluorophosphate (HIP) as the initiator, enabling the printing of multi-layer structures in the porous support.
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http://dx.doi.org/10.1039/d1cc01383aDOI Listing
May 2021

Gelatinase-responsive release of an antibacterial photodynamic peptide against .

Biomater Sci 2021 May;9(9):3433-3444

Department of Chemistry, the Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Staphylococcus aureus (S. aureus) related staphylococcal infection is one of the most common types of hospital-acquired infections, which requires selective and effective treatment in clinical practice. Considering gelatinase as a characteristic feature of S. aureus, gelatinase-responsive release of the antibiotic reagent thereby can target the pathogenic S. aureus while sparing beneficial bacteria in the microflora. In this work, we design a hybrid antibacterial photodynamic peptide (APP, Ce6-GKRWWKWWRRPLGVRGC) based on the polycationic antimicrobial peptide GKRWWKWWRR by introducing a photosensitizer chlorin e6 (Ce6) at the N-terminus, a cysteine residue at the C-terminus, and a gelatinase cleavage site (PLGVRG) inserted between the C-terminal cysteine and the polycationic peptide. This multi-motif peptide assembles with gold nanoclusters (AuNc) via Au-thiol bonding and affords a gelatinase-responsive antibacterial photodynamic nanocomposite (GRAPN). In vitro results show that the gelatinase secreted by S. aureus can cleave and release APP from AuNc, thereby resulting in preferential killing of S. aureus over E. coli. In a mouse model of staphylococcal skin wound infection, by integrating gelatinase-responsive drug release and the synergistic effect of a photodynamic agent and APP, GRAPN exhibits a marked photodynamic antibacterial activity, effectively eradicates S. aureus infection, and promotes rapid healing of the infected wounds.
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http://dx.doi.org/10.1039/d0bm02201bDOI Listing
May 2021

CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance.

Cell Stem Cell 2021 Apr 28. Epub 2021 Apr 28.

Laboratory of Molecular Signaling, Division of Oral Biology and Medicine, School of Dentistry, Jonsson Comprehensive Cancer Center and Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address:

Immunosurveillance is a critical mechanism guarding against tumor development and progression. Checkpoint inhibitors have shown significant success in cancer treatment, but expression of key factors such as PD-L1 in putative cancer stem cell (CSC) populations in squamous cell carcinoma has been inconclusive, suggesting that CSCs may have developed other mechanisms to escape immune surveillance. Here we show that CSCs upregulate the immune checkpoint molecule CD276 (B7-H3) to evade host immune responses. CD276 is highly expressed by CSCs in mouse and human head and neck squamous cell carcinoma (HNSCC) and can be used to prospectively isolate tumorigenic CSCs. Anti-CD276 antibodies eliminate CSCs in a CD8 T cell-dependent manner, inhibiting tumor growth and lymph node metastases in a mouse HNSCC model. Single-cell RNA sequencing (RNA-seq) showed that CD276 blockade remodels SCC heterogeneity and reduces epithelial-mesenchymal transition. These results show that CSCs utilize CD276 for immune escape and suggest that targeting CD276 may reduce CSCs in HNSCC.
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http://dx.doi.org/10.1016/j.stem.2021.04.011DOI Listing
April 2021

Tuning the Topology of Three-Dimensional Covalent Organic Frameworks via Steric Control: From to Unprecedented .

J Am Chem Soc 2021 May 4. Epub 2021 May 4.

Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Whether or not the topology of three-dimensional covalent organic frameworks (3D COFs) can be tuned via steric control remains a big question and has never been reported. Herein, we describe the designed synthesis of two highly crystalline 3D COFs (3D-TPB-COF-OMe and 3D-TPB-COF-Ph), through the polycondensation of tetra(-aminophenyl)methane and methoxy- or phenyl- substituted 1,2,4,5-tetrakis(4-formylphenyl)benzene on the 3- and 6-positions. Amazingly, by using the continuous rotation electron diffraction technique, 3D-TPB-COF-OMe is determined to have a 5-fold interpenetrated structure with a reported net, while 3D-TPB-COF-Ph adopts an unprecedented self-penetrated topology ( = Luojia Hill) that does not exist in the database of ToposPro. Therefore, by altering the substituents from methoxy to phenyl groups, the topology of designed 3D COFs changes accordingly, and a rare net is now available. This result clearly demonstrates that such COF structures need to be carefully determined due to its complexity, and moreover, it is promising to design 3D COFs with new topology for interesting application by increasing the steric hindrance of molecular building blocks.
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http://dx.doi.org/10.1021/jacs.1c03042DOI Listing
May 2021

A novel MYC-non-IG fusion in refractory diffuse large B-cell lymphoma.

Br J Haematol 2021 May 3. Epub 2021 May 3.

Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.

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http://dx.doi.org/10.1111/bjh.17255DOI Listing
May 2021

A Super-Enhancer Driven by FOSL1 Controls miR-21-5p Expression in Head and Neck Squamous Cell Carcinoma.

Front Oncol 2021 16;11:656628. Epub 2021 Apr 16.

Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China.

MiR-21-5p is one of the most common oncogenic miRNAs that is upregulated in many solid cancers by inhibiting its target genes at the posttranscriptional level. However, the upstream regulatory mechanisms of miR-21-5p are still not well documented in cancers. Here, we identify a super-enhancer associated with the gene (MIR21-SE) by analyzing the genomic regulatory landscape in head and neck squamous cell carcinoma (HNSCC). We show that the MIR21-SE regulates miR-21-5p expression in different HNSCC cell lines and disruption of MIR21-SE inhibits miR-21-5p expression. We also identified that a key transcription factor, FOSL1 directly controls miR-21-5p expression by interacting with the MIR21-SE in HNSCC. Moreover, functional studies indicate that restoration of miR-21-5p partially abrogates FOSL1 depletion-mediated inhibition of cell proliferation and invasion. Clinical studies confirmed that miR-21-5p expression is positively correlated with FOSL1 expression. These findings suggest that FOSL1-SE drives miR-21-5p expression to promote malignant progression of HNSCC.
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http://dx.doi.org/10.3389/fonc.2021.656628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085558PMC
April 2021

A Wearable Gait Analysis System Used in Type 2 Diabetes Mellitus Patients: A Case-Control Study.

Diabetes Metab Syndr Obes 2021 23;14:1799-1808. Epub 2021 Apr 23.

Department of Neurosurgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Background: Previous studies have shown that the gait of patients with type-2 diabetes mellitus is abnormal compared with the healthy group. Currently, a three-dimensional motion analyzer system is commonly used for gait analysis. However, it is challenging to collect data and use in clinical study due to extensive experimental conditions and high price. In this study, we used a wearable gait analysis system (Gaitboter) to investigate the spatial and temporal parameters, and kinematic data of gait in diabetic patients, especially those with peripheral neuropathy. The aim of the study is to evaluate the wearable gait analysis system in diabetic study.

Materials And Methods: We conducted a case-control study to analyze the gait of type 2 diabetes mellitus. Gaitboter was used to detect and collect gait data in the ward of Beijing Chao-yang Hospital, Capital Medical University from June 2018 to October 2018. We collected the gait data of participants (N= 146; 73 patients with type 2 diabetes, 16 with peripheral neuropathy and 57 without peripheral neuropathy, and 73 matched controls). The gait data (stance phase, swing phase, double-foot stance phase, single-foot stance phase, walking cadence, stride length, walking speed, off-ground angle, landing angle, maximum swing angle, minimum swing angle, and foot progression angle) in diabetic patients were recorded and compared with controls. SPSS 22.0 statistical software was used to analyzed the gait parameter data.

Results: We found that the landing angle and the maximum swing angle of diabetes patients with or without peripheral neuropathy were significantly less than those of the control group (P < 0.05). The walking speed of diabetes patients with peripheral neuropathy is significantly less than those of the control group (P < 0.05).

Conclusion: This study confirms that the wearable gait analysis system (Gaitboter) is an ideal system to identify abnormal gait in patients with type 2 diabetes and provides a new device and method for diabetes-related gait research.
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http://dx.doi.org/10.2147/DMSO.S305102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079258PMC
April 2021

A novel multi-DoF surgical robotic system for brachytherapy on liver tumor: Design and control.

Int J Comput Assist Radiol Surg 2021 May 1. Epub 2021 May 1.

Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, GD, 518055, People's Republic of China.

Purpose: Radioactive seed implantation is an effective invasive treatment method for malignant liver tumors in hepatocellular carcinomas. However, challenges of the manual procedure may degrade the efficacy of the technique, such as the high accuracy requirement and radiation exposure to the surgeons. This paper aims to develop a robotic system and its control methods for assisting surgeons on the treatment.

Method: We present an interventional robotic system, which consists of a 5 Degree-of-Freedom (DoF) positioning robotic arm (a 3-DoF translational joint and a 2-DoF revolute joint) and a needle actuator used for needle insertion and radioactive seeds implantation. Control strategy is designed for the system to ensure the safety of the motion. In the designed framework, an artificial potential field (APF)-based motion planning and an ultrasound (US) image-based contacting methods are proposed for the control.

Result: Experiments were performed to evaluate position and orientation accuracy as well as validate the motion planning procedure of the system. The mean and standard deviation of targeting error is 0.69 mm and 0.33 mm, respectively. Needle placement accuracy is 1.10 mm by mean. The feasibility of the control strategy, including path planning and the contacting methods, is demonstrated by simulation and experiments based on an abdominal phantom.

Conclusion: This paper presents a robotic system with force and US image feedback in assisting surgeons performing brachytherapy on liver tumors. The proposed robotic system is capable of executing an accurate needle insertion task with by optical tracking. The proposed methods improve the safety of the robot's motion and automate the process of US probe contacting under the feedback of US-image.
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http://dx.doi.org/10.1007/s11548-021-02380-7DOI Listing
May 2021

Study on habitat suitability and environmental variable thresholds of rare waterbirds.

Sci Total Environ 2021 Apr 24;785:147316. Epub 2021 Apr 24.

Jiangsu Yancheng Wetland National Nature Reserve, Rare Birds, Yancheng, Jiangsu 224057, China.

The conservation level of rare waterbirds reflects the quality of the regional ecological environment and wetlands, and suitable habitat patches and good environmental conditions are bases to support the activities of rare species in habitats. Establishing these conditions is also an important goal of habitat landscape and functional restoration. However, lack of these conditions limits population protection and habitat restoration of rare species. Based on the random forest (RF) algorithm and threshold indicator taxa analysis (TITAN), this paper performed habitat suitability assessment and environmental variable threshold analysis of rare waterbird species in Yancheng coastal wetlands. The results showed that the suitable area proportion of three waterbird species at different habitat sites was less than 20%. The unsuitable area proportions of red-crowned cranes and oriental storks at the CA habitat site were the highest, reaching 86.73% and 85.17%, respectively. In addition, analysis of the importance of environmental variables showed that the main influencing variables affecting the suitable habitat distribution of the three rare waterbirds were habitat type (T_hab), habitat area (A_hab), vegetation coverage (P_fvc), distance to farmland (D_far), distance to reeds (D_ree), ponds density (Ponds), distance to water surface (D_wat) and distance to main roads or seawalls (D_swa). These variables covered the type, area, coverage and distance indicators. With the exception of D_far, Ponds and D_swa, rare waterbirds had response thresholds to each environmental indicator, and these results supported the restoration of landscape structure and function of each habitat site. This study emphasized the importance of foods, water resources and hidden conditions for habitat selection in rare waterbirds. Finally, we proposed the maintenance and restoration patterns of the landscape structure and function of rare waterbird habitats, which are available for other coastal tidal wetlands.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147316DOI Listing
April 2021

Astemizole as a drug to inhibit the effect of SARS-COV-2 in vitro.

Microb Pathog 2021 Apr 29;156:104929. Epub 2021 Apr 29.

School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China. Electronic address:

Since the beginning of December 2019, a novel Coronavirus severe respiratory disease, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) which also been termed 2019-new CoV (2019-nCoV), has continued to spread worldwide. As of August 27, 2020, a total of 24,232,429 people have been infected and 826,518 people have died. In our study, we found that astemizole can antagonize ACE2 and inhibit the entry of SARS-COV-2 spike pseudovirus into ACE2-expressed HEK293T cells (ACE2hi cells). We analysied the binding character of astemizole to ACE2 by molecular docking and surface plasmon resonance (SPR) assays and molecule docking, SARS-COV-2 spike pseudotype virus was also taken to investigate the suppression viropexis effect of astemizole. The results showed that astemizole can bind to the ACE2 receptor and inhibit the invasion of SARS-COV-2 Spike pseudoviruses. Thus astemizole represent potential drug candidates that can be re-used in anti-coronavirus therapies.
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http://dx.doi.org/10.1016/j.micpath.2021.104929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081582PMC
April 2021

Low-pressure pulse flushing choledochoscopy combined with neodymium laser lithotripsy for the treatment of intrahepatic bile duct stones.

Hepatobiliary Pancreat Dis Int 2021 Apr 16. Epub 2021 Apr 16.

Department of Hepatobiliary Surgery, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Taizhou 317000, China. Electronic address:

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http://dx.doi.org/10.1016/j.hbpd.2021.04.002DOI Listing
April 2021

The breadth of concomitant virological features in a family cluster outbreak of COVID-19 pneumonia.

J Med Virol 2021 Apr 30. Epub 2021 Apr 30.

Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, 100039, P.R. China.

An outbreak of a novel coronavirus disease (COVID-19) caused by SARS-CoV-2 had emerged in 2019 and rapidly posed a global epidemic. Here, we report the breadth of concomitant virological features of a family cluster with COVID-19. The period of virus shedding is significantly different between upper respiratory and feces samples. Even the SARS-CoV-2 virus titers were undetectable in feces, it could be positive again soon and likely related to fluctuated inflammation levels (IL-6, etc.) and lowered immune responses (CD4+ T lymphocyte, etc.). Our findings expand the novel understanding of the breadth of concomitant virological features during a non-severe family cluster of COVID-19. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jmv.27055DOI Listing
April 2021

Mixed Solvothermal Synthesis of T Cluster-Based Indium and Gallium Sulfides Using Versatile Ammonia or Amine Structure-Directing Agents.

Inorg Chem 2021 Apr 29. Epub 2021 Apr 29.

Tianjin Key Laboratory of Advanced Functional Porous Materials, Institute for New Energy Materials and Low-Carbon Technologies, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China.

Metal chalcogenide supertetrahedral T clusters are of current interest for their unique compositions and structures, which rely highly on the structure-directing agents. Herein, we report four novel T cluster-based indium and gallium sulfides, namely, [NH(CH)]InSH (), (NH)GaS (), [NHCHCH](NHCHCH)GaS (), and [NHCHCHOH]GaS·2NHCHCHOH (). All four compounds were solvothermally synthesized in mixed amine-ethanol solutions or deep eutectic solvent (DES), where ammonia/amine molecules play significant structure-directing roles in the speciation and crystal growth. (1) Being protonated, the trimethylamine and ethanolamine molecules surround the T2-[InSH] clusters (for ) and [GaS] open framework (for ), respectively, compensating for the negative charge of the inorganic moieties. (2) With the lone pair of electrons, the ammonia molecules in coordinate directly to corner Ga ions of the {GaS} cage to give a neutral T2-(NH)GaS cluster. (3) For compound , part of the ethylamine molecules act as terminating ligands for the T1 and T3 units in the [GaS(NHCHCH)] layer, while the rest act as interlamellar countercations upon protonation. Theoretical studies reveal the contributions of N, C, and H to the density of states (DOS) for and because of their hybrid structures that combine the ammonia/amine ligands with sulfide moieties together.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00171DOI Listing
April 2021

Surface Plasmon Resonance Boost Electrocatalytic Alcohol Oxidation over Three-Dimensional PdM (M = Au, Ag, Cu) Nanosheet Assemblies.

Inorg Chem 2021 Apr 28. Epub 2021 Apr 28.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Renai Road, Suzhou 215123, P. R. China.

Photoelectrocatalytic nanomaterials are promising for direct alcohol fuel cells, but the construction of high-efficiency catalysts remains difficult. We herein successfully synthesized three-dimensional (3D) PdM nanosheet assemblies (PdM NSAs, M = Au, Ag, and Cu) through a seed-mediated growth method, which displayed a typical 3D nanoflower morphology assembled from many two-dimensional ultrathin nanosheets. Due to the open 3D structure and the synergistic and electronic effects between Pd and Ag, the optimized PdAg NSAs showed the highest mass activity (9378 mA mg) for the ethylene glycol oxidation reaction. More interestingly, when irradiated with visible light, the mass activity increased to 14 590 mA mg, 12.1 times higher than that of the commercial Pd/C (1205 mA mg). In addition, the as-obtained catalysts also showed better long-term durability than that of the commercial Pd/C under the condition of with or without visible-light illumination. This work highlights the utilization of light energy in designing excellent photoelectrocatalysts to promote the photoelectrocatalytic performance of anode catalysts for fuel cells.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00885DOI Listing
April 2021

Correction to: Chemopreventive effect of Betulinic acid via mTOR -Caspases/Bcl2/Bax apoptotic signaling in pancreatic cancer.

BMC Complement Med Ther 2021 Apr 26;21(1):131. Epub 2021 Apr 26.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, China.

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http://dx.doi.org/10.1186/s12906-021-03254-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074483PMC
April 2021

Genome of the butterfly hillstream loach provides insights into adaptations to torrential mountain stream life.

Mol Ecol Resour 2021 Apr 23. Epub 2021 Apr 23.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Butterfly hillstream loach (Beaufortia kweichowensis), a benthic fish in the torrential mountain streams, possesses a totally flat ventrum, flattened craniofacial and body skeletons, and enlarged paired fins covered by substantially small keratinous structures. However, little is known about the genetic basis of these specialized morphological adaptations. Here we present a 448.52-Mb genome assembly with contig N50 length of 5.53 Mb by integrating Illumina short-read sequencing, Nanopore long-read sequencing and HiC-based chromatin map. Demographic history reconstruction of the butterfly hillstream loach reveals that the population dynamics is correlated with the different stages of uplifting of the Tibetan Plateau. Comparative genomic analysis finds evidence of six keratin genes in butterfly hillstream loach evolving under positive selection. Within these genes, two keratin genes exhibit species-specific and divergent amino acid changes, suggesting a role in the formation of the unculi. Additionally, a series of positively selected genes, rapid evolving genes, specific variant genes and expanded gene families are found, including genes related to Hedgehog, Notch and BMP pathways, which may be involved in craniofacial development. These findings may have important implications for understanding the genetic basis of phenotypic adaptation to torrential mountain stream life.
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http://dx.doi.org/10.1111/1755-0998.13400DOI Listing
April 2021

A general MOF-intermediated synthesis of hollow CoFe-based trimetallic phosphides composed of ultrathin nanosheets for boosting water oxidation electrocatalysis.

Nanoscale 2021 Apr 12;13(15):7279-7284. Epub 2021 Apr 12.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.

Engineering an electrode material for boosting reaction kinetics is highly desired for the oxygen evolution reaction (OER) in the anodic half reaction, and is still a grand challenge for energy conversion technologies. By taking inspiration from the catalytic properties of transition metal phosphides (TMPs) and metal-organic frameworks (MOFs), we herein propose a general MOF-intermediated synthesis of a series of hollow CoFeM (M = Bi, Ni, Mn, Cu, Ce, and Zn) trimetallic phosphides composed of ultrathin nanosheets as advanced electrocatalysts for the OER. A dramatic improvement of electrocatalytic performance toward the OER is observed for hollow CoFeM trimetallic phosphides compared to bimetallic CoFe phosphides. Remarkably, composition-optimized CoFeBiP hollow microspheres could deliver superior electrocatalytic performance, achieving a current density of 10 mA cm with an overpotential of only 273 mV. Mechanistic investigations reveal that the Bi and P doping effectively optimizes the electronic structure of Co and Fe by charge redistribution, which significantly lowers the adsorption energy of oxygen intermediates. Moreover, the hollow microsphere structures composed of ultrathin nanosheets also enable them to provide rich surface active sites to boost the electrocatalytic OER.
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http://dx.doi.org/10.1039/d1nr00075fDOI Listing
April 2021

Non-alcoholic fatty liver disease is a risk factor for occurrence of hepatocellular carcinoma after sustained virologic response in chronic hepatitis C patients: A prospective four-years follow-up study.

Metabol Open 2021 Jun 26;10:100090. Epub 2021 Mar 26.

Department of Liver Diseases, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100039, China.

Background And Aim: The incidence of hepatocellular carcinoma (HCC) decreases significantly in chronic hepatitis C (CHC) patients with sustained virologic response (SVR) after pegylated-interferon plus ribavirin (PR) or direct-acting antiviral (DAAs) therapy. We follow-up a single cohort of CHC patients to identify risk factors associated with HCC development post-SVR.

Method: CHC patients with SVR in Beijing/Hong Kong were followed up at 12-24 weekly intervals with surveillance for HCC by ultrasonography and alpha-fetoprotein (AFP). Multivariate Cox proportional hazards regression analysis was used to explore factors associated with HCC occurrence.

Results: Between October 2015 and May 2017, SVR was observed in 519 and 817 CHC patients after DAAs and PR therapy respectively. After a median post -SVR follow-up of 48 months, HCC developed in 54 (4.4%) SVR subjects. By adjusted Cox analysis, older age (≥55 years) [HR 2.4, 95% CI (1.3-4.3)], non-alcoholic fatty liver diseases [HR 2.4, 95%CI (1.3-4.2), higher AFP level (≥20 ng/ml) [HR 3.4, 95%CI (2.0-5.8)], higher liver stiffness measurement (≥14.6 kPa) [HR 4.2, 95%CI (2.3-7.6)], diabetes mellitus [HR 4.2, 95%CI (2.4-7.4)] at pre-treatment were associated with HCC occurrence. HCC patients in the DAAs induced SVR group had a higher prevalence of NAFLD as compared with those in the PR induced SVR group, 62% (18/29) vs 28% (7/25), p = 0.026. A nomogram formulated with the above six independent variables had a Concordance-Index of 0.835 (95% CI 0.783-0.866).

Conclusion: Underlying NAFLD is associated with increased incidence of HCC in chronic HCV patients post-SVR, particularly in those treated with DAA.
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http://dx.doi.org/10.1016/j.metop.2021.100090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050772PMC
June 2021

An injectable double-crosslinking iodinated composite hydrogel as a potential radioprotective spacer with durable imaging function.

J Mater Chem B 2021 Apr 6;9(15):3346-3356. Epub 2021 Apr 6.

College of Materials Science & Engineering, Zhejiang University of Technology, China.

Prostate cancer is one of the leading causes of cancer incidence among males worldwide. Radiotherapy can achieve similar oncological outcomes to those of radical prostatectomy. One concern is, however, radiation damage to the rectum because of the extreme proximity between the two organs. Inserting a biomaterial to separate the prostate and rectum is a promising strategy, and an injectable hydrogel is regarded to be the preferred spacer after screening of various materials. Nevertheless, there exist shortcomings for the currently available injectable hydrogel that cannot fully meet the unique requirements in clinical practice. In this work, a novel injectable hydrogel spacer based on carboxymethyl chitosan (CMC), aldehyde guar gum (AG), and aldehyde iohexol (DHQ) with an imaging function is fabricated. Contrast agent DHQ is chemically attached to CMC-AG network to form a double-crosslinking network to obtain a controlled degradation rate and high strength as well as durable CT imaging function. The hydrogel is injected subcutaneously into rats, where rapid gelation occurs and it serves as a hydrogel spacer. During the month-long in vivo studies, the spacer exhibits remarkable radiation dose attenuation and sustainable imaging function, as well as excellent toxicity profiles. This novel hydrogel shows excellent potential in the protection of critical organs during prostate cancer radiotherapy.
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http://dx.doi.org/10.1039/d0tb02953jDOI Listing
April 2021

Advances in hydrogen production from electrocatalytic seawater splitting.

Nanoscale 2021 May;13(17):7897-7912

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.

As one of the most abundant resources on the Earth, seawater is not only a promising electrolyte for industrial hydrogen production through electrolysis, but also of great significance for the refining of edible salt. Despite the great potential for large-scale hydrogen production, the implementation of water electrolysis requires efficient and stable electrocatalysts that can maintain high activity for water splitting without chloride corrosion. Recent years have witnessed great achievements in the development of highly efficient electrocatalysts toward seawater splitting. Starting from the historical background to the most recent achievements, this review will provide insights into the current state, challenges, and future perspectives of hydrogen production through seawater electrolysis. In particular, the mechanisms of overall water splitting, key features of seawater electrolysis, noble-metal-free electrocatalysts for seawater electrolysis and the underlying mechanisms are also highlighted to provide guidance for fabricating more efficient electrocatalysts toward seawater splitting.
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http://dx.doi.org/10.1039/d1nr00784jDOI Listing
May 2021

Cortically constrained shape recognition: Automated white matter tract segmentation validated in the pediatric brain.

J Neuroimaging 2021 Apr 20. Epub 2021 Apr 20.

Department of Neurology, University of California, UCSF Memory and Aging Center, Sandler Neurosciences Center, San Francisco, California, USA.

Background And Purpose: Manual segmentation of white matter (WM) bundles requires extensive training and is prohibitively labor-intensive for large-scale studies. Automated segmentation methods are necessary in order to eliminate operator dependency and to enable reproducible studies. Significant changes in the WM landscape throughout childhood require flexible methods to capture the variance across the span of brain development.

Methods: Here, we describe a novel automated segmentation tool called Cortically Constrained Shape Recognition (CCSR), which combines two complementary approaches: (1) anatomical connectivity priors based on FreeSurfer-derived regions of interest and (2) shape priors based on 3-dimensional streamline bundle atlases applied using RecoBundles. We tested the performance and repeatability of this approach by comparing volume and diffusion metrics of the main language WM tracts that were both manually and automatically segmented in a pediatric cohort acquired at the UCSF Dyslexia Center (n = 59; 25 females; average age: 11 ± 2; range: 7-14).

Results: The CCSR approach showed high agreement with the expert manual segmentations: across all tracts, the spatial overlap between tract volumes showed an average Dice Similarity Coefficient (DSC) of 0.76, and the fractional anisotropy (FA) on average had a Lin's Concordance Correlation Coefficient (CCC) of 0.81. The CCSR's repeatability in a subset of this cohort achieved a DSC of 0.92 on average across all tracts.

Conclusion: This novel automated segmentation approach is a promising tool for reproducible large-scale tractography analyses in pediatric populations and particularly for the quantitative assessment of structural connections underlying various clinical presentations in neurodevelopmental disorders.
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http://dx.doi.org/10.1111/jon.12854DOI Listing
April 2021