Publications by authors named "Cheng Ma"

341 Publications

Not by Serendipity: Rationally Designed Reversible Temperature-Responsive Circularly Polarized Luminescence Inversion by Coupling Two Scenarios of Harata-Kodaka's Rule.

JACS Au 2021 Feb 15;1(2):156-163. Epub 2020 Dec 15.

Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Intelligent control over the handedness of circularly polarized luminescence (CPL) is of special significance in smart optoelectronics, information storage, and data encryption; however, it still remains a great challenge to rationally design a CPL material that displays reversible handedness inversion without changing the system composition. Herein, we show this comes true by coupling the two scenarios of Harata-Kodaka's rule on the same supramolecular platform of crystalline microtubes self-assembled from surfactant-cyclodextrin host-guest complexes. Upon coassembling a linear dye with its electronic transition dipole moment outside of the cavity of β-CyD, the chirality transfer from the induced chirality of SDS in the [email protected]β-CyD microtubes to the dye generates left-handed CPL at room temperature. Upon elevating temperature, the dye forms inclusion complex with β-CyD, so that right-handed CPL is induced because the polar group of the dye is outside of the cavity of β-CyD. This process is completely reversible. We envision that host-guest chemistry would be very promising in creating smart CPL inversion materials for a vast number of applications.
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http://dx.doi.org/10.1021/jacsau.0c00061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395654PMC
February 2021

Vaccine Composition Formulated with a Novel -Derived Exopolysaccharides Adjuvant Provided High Protection against .

Vaccines (Basel) 2021 Jul 12;9(7). Epub 2021 Jul 12.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China.

A vaccine that effectively targets methicillin-resistant (MRSA) is urgently needed, and has been the focus of studies by numerous research groups, but with limited success to date. Recently, our team found that exopolysaccharides derived from probiotic strain WXD030 as an adjuvant-formulated OVA could upregulate IFN-γ and IL-17 expression in CD4 T cells. In this study, we developed a vaccine (termed rMntC-EPS) composed of antigen MntC and   exopolysaccharides, which conferred high levels of protection against infection.

Methods: Six-eight-week-old female mice were vaccinated with purified rMntC-EPS30. The immune protection function of rMntC-EPS30 was assessed by the protective effect of rMntC-EPS30 to -induced pulmonary and cutaneous infection in mice, bacterial loads and H&E in injury site, and ELISA for inflammation-related cytokines. The protective mechanism of rMntC-EPS30 was assessed by ELISA for IgG in serum, cytokines in the spleen and lungs of vaccinated mice. In addition, flow cytometry was used for analyzing cellular immune response induced by rMntC-EPS30. For confirmation of our findings, three kinds of mice were used in this study: IL-17A knockout mice, IFN-γ knockout mice and TCRγ/δ knockout mice.

Results: rMntC-EPS30 conferred up to 90% protection against pulmonary infection and significantly reduced the abscess size in the cutaneous model, with clearance of the pathogen. The rMntC-EPS vaccine could induce superior humoral immunity as well as significantly increase IL-17A and IFN-γ production. In addition, we found that rMntC-EPS vaccination induced robust Th 17/γδ T 17 primary and recall responses. Interestingly, this protective effect was distinctly reduced in the IL-17A knockout mice but not in IFN-γ knockout mice. Moreover, in TCRγ/δ knockout mice, rMntC-EPS vaccination neither increased IL-17A secretion nor provided effective protection against infection.

Conclusions: These data demonstrated that the rMntC formulated with a novel -derived Exopolysaccharides adjuvant provided high protection against . The rMntC-EPS vaccine induced γδ T cells and IL-17A might play substantial roles in anti- immunity. Our findings provided direct evidence that rMntC-EPS vaccine is a promising candidate for future clinical application against -induced pulmonary and cutaneous infection.
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http://dx.doi.org/10.3390/vaccines9070775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310297PMC
July 2021

Impact of federal mandates on demographic reporting in rhinitis clinical trials.

Int Forum Allergy Rhinol 2021 Aug 6. Epub 2021 Aug 6.

Department of Otolaryngology-Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina, USA.

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http://dx.doi.org/10.1002/alr.22876DOI Listing
August 2021

Hydrogen sulfide and nitric oxide are involved in melatonin-induced salt tolerance in cucumber.

Plant Physiol Biochem 2021 Jul 28;167:101-112. Epub 2021 Jul 28.

School of Life Sciences, Northwest Normal University, Lanzhou, 730070, China. Electronic address:

Hydrogen sulfide (HS) is a novel gaseous signaling molecule in response to adversity stress. Melatonin (MT) is a multifunctional molecule that plays an important role in regulating plant stress resistance. However, the interactions between HS and MT are still unknown. Therefore, the role of HS in MT-induced salt tolerance was elucidated in this study by measuring the antioxidant defense system and photosynthetic characteristics of cucumber. In addition, the crosstalk among HS, NO, and mitogen-activated protein kinase (MAPK) was investigated. Results showed that MT induced the production of HS by significantly increasing the activity of L-/D-cysteine desulfhydrase, thereby regulating photosynthetic efficiency, antioxidant enzyme activity, and antioxidant enzyme gene expression in cucumber, thus alleviating reactive oxygen species burst by salt stress. In this process, the HS and NO induced by MT were inhibited by NO scavenger (cPTIO) and HS scavenger (HT) but not affected by MAPK inhibitor (U0126). Intriguingly, the expression of MAPK3/4/6/9 was inhibited by HT and cPTIO. These results suggested that HS may act as downstream of MT, interact with NO and MAPK cascades, and jointly participate in the process of MT mitigating salt stress in cucumber. In addition, HS and NO are upstream signaling molecules of the MAPK cascades.
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http://dx.doi.org/10.1016/j.plaphy.2021.07.023DOI Listing
July 2021

[Comparison of two methods for preparing knee osteochondral injury models in mice].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jul;35(7):862-867

Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu, 210000, P.R.China.

Objective: To observe the effect of using tungsten drills to prepare mouse knee osteochondral injury model by comparing with the needle modeling method, in order to provide an appropriate animal modeling method for osteochondral injury research.

Methods: A total of 75 two-month-old male C57BL/6 mice were randomly divided into 3 groups ( =25). Mice in groups A and B were used to prepare the right knee osteochondral injury models by using needles and tungsten drills, respectively; group C was sham-operation group. The general condition of the mice was observed after operation. The samples were taken at 1 day and 1, 2, 4, and 8 weeks after modeling, and HE staining was performed. The depth, width, and cross-sectional area of the injury site at 1 day in groups A and B were measured, and the percentage of the injury depth to the thickness of the articular cartilage (depth/thickness) was calculated. Toluidine blue staining and immunohistochemical staining for collagen type Ⅱ were performed at 8 weeks, and the International Cartilage Research Society (ICRS) score was used to evaluate the osteochondral healing in groups A and B.

Results: All mice survived to the completion of the experiment. HE staining showed that group C had normal cartilage morphology. At 1 day after modeling, the injury in group A only broke through the cartilage layer and reached the subchondral bone without entering the bone marrow cavity; the injury in group B reached the bone marrow cavity. The depth, width, cross-sectional area, and depth/thickness of the injury in group A were significantly lower than those in group B ( <0.05). At 1, 2, 4, and 8 weeks after modeling, there was no obvious tissue filling in the injured part of group A, and no toluidine blue staining and expression of collagen type Ⅱ were observed at 8 weeks; while the injured part of group B was gradually filled with tissue, the toluidine blue staining and the expression of collagen type Ⅱ were seen at 8 weeks. At 8 weeks, the ICRS score of group A was 8.2±1.3, which was lower than that of group B (13.6±0.9), showing significant difference ( =-7.637, =0.000).

Conclusion: The tungsten drills can break through the subchondral bone layer and enter the bone marrow cavity, and the injury can heal spontaneously. Compared with the needle modeling method, it is a better method for modeling knee osteochondral injury in mice.
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http://dx.doi.org/10.7507/1002-1892.202101098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311214PMC
July 2021

A cost-effective and humidity-tolerant chloride solid electrolyte for lithium batteries.

Nat Commun 2021 Jul 20;12(1):4410. Epub 2021 Jul 20.

Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Materials for Energy Conversion, Department of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui, China.

Li-ion-conducting chloride solid electrolytes receive considerable attention due to their physicochemical characteristics such as high ionic conductivity, deformability and oxidative stability. However, the raw materials are expensive, and large-scale use of this class of inorganic superionic conductors seems unlikely. Here, a cost-effective chloride solid electrolyte, LiZrCl, is reported. Its raw materials are several orders of magnitude cheaper than those for the state-of-the-art chloride solid electrolytes, but high ionic conductivity (0.81 mS cm at room temperature), deformability, and compatibility with 4V-class cathodes are still simultaneously achieved in LiZrCl. Moreover, LiZrCl demonstrates a humidity tolerance with no sign of moisture uptake or conductivity degradation after exposure to an atmosphere with 5% relative humidity. By combining LiZrCl with the Li-In anode and the single-crystal LiNiMnCoO cathode, we report a room-temperature all-solid-state cell with a stable specific capacity of about 150 mAh g for 200 cycles at 200 mA g.
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http://dx.doi.org/10.1038/s41467-021-24697-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292426PMC
July 2021

On-Demand Storage and Retrieval of Microwave Photons Using a Superconducting Multiresonator Quantum Memory.

Phys Rev Lett 2021 Jul;127(1):010503

Center for Quantum Information, Institute for Interdisciplinary Information Sciences, Tsinghua University, Beijing 100084, People's Republic of China.

We realize on-demand storage and retrieval of weak coherent microwave photon pulses at the single-photon level. A superconducting multiresonator system which is composed of a set of frequency-tunable coplanar waveguide resonators is implemented as the quantum memory. By dynamically tuning the resonant frequencies of the resonators, we achieve tunable memory bandwidth from 10 to 55 MHz, with well preserved phase coherence. We further demonstrate on-demand storage and retrieval of a time-bin flying qubit. This result opens up a prospect to integrate our chip-based quantum memory with the state-of-the-art superconducting quantum circuit technology for quantum information processing.
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http://dx.doi.org/10.1103/PhysRevLett.127.010503DOI Listing
July 2021

The Effect of CPAP on Middle Ear Pressure: A Systematic Review and Meta-analysis.

Otol Neurotol 2021 Oct;42(9):1300-1307

Medical University of South Carolina, Department of Otolaryngology-Head and Neck Surgery, Charleston, South Carolina.

Objective: To assess the effect of short-term and long-term continuous positive airway pressure (CPAP) use on middle ear pressure (MEP) in adult ears.

Databases Reviewed: PubMed, Scopus, and Embase were searched through October 2020. Search strategies used a combination of subject headings (e.g., MeSH in PubMed) and keywords for CPAP and middle ear pressure.

Methods: English-language articles studying changes in MEP as measured by tympanometry or tympanograms in patients using CPAP were selected for inclusion. Data extraction from included articles included demographics, baseline, and follow-up data.

Results: A total of 7 articles representing 664 patients with weighted mean age of 42.5 years (range 34.9-57.8 yrs) were included. Meta-analysis of three qualifying studies demonstrated a positive correlation between higher CPAP pressures and degree of MEP rise during CPAP use compared to baseline with mean difference of 25.1 daPa (95% confidence interval [18.7, 31.4]) at 5 cm H2O and 81.9 daPa (95% confidence interval [60.2, 103.5]) at 10 cm H2O. Short-term effects of CPAP use were reviewed in four studies that showed a transient rise in MEP. Three separate studies examining the long-term effects of CPAP use showed resting MEP was higher in patients with severe obstructive sleep apnea (OSA), which might be related to progressive eustachian tube dysfunction as a complication of severe OSA.

Conclusion: Short-term CPAP use is associated with transient elevations of MEP in adult ears. Long-term CPAP use might produce beneficial MEP changes, especially in patients with OSA and eustachian tube dysfunction.
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http://dx.doi.org/10.1097/MAO.0000000000003276DOI Listing
October 2021

Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease.

Bio Protoc 2021 Jun 20;11(12):e4059. Epub 2021 Jun 20.

Department of Pharmacology and Chemical Biology, Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA.

Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of disease and control samples, analysis of the large datasets has become a challenge. Here, we provide a protocol for the systems-level analysis of N-glycosylation sites on N-glycosylated proteins and their changes in human disease, such as Alzheimer's disease. The protocol includes quantitation and differential analysis of N-glycopeptide abundance, in addition to integrative N-glycoproteome and proteome data analyses, to determine disease-associated changes in N-glycosylation site occupancy and identify differentially N-glycosylated proteins in human disease versus control samples. This protocol can be modified and applied to study proteome-wide N-glycosylation alterations in response to different cellular stresses or pathophysiological states in other organisms or model systems.
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http://dx.doi.org/10.21769/BioProtoc.4059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260255PMC
June 2021

Intranasal delivery of interleukin-4 attenuates chronic cognitive deficits via beneficial microglial responses in experimental traumatic brain injury.

J Cereb Blood Flow Metab 2021 Jul 14:271678X211028680. Epub 2021 Jul 14.

Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA, USA.

Traumatic brain injury (TBI) is commonly followed by long-term cognitive deficits that severely impact the quality of life in survivors. Recent studies suggest that microglial/macrophage (Mi/MΦ) polarization could have multidimensional impacts on post-TBI neurological outcomes. Here, we report that repetitive intranasal delivery of interleukin-4 (IL-4) nanoparticles for 4 weeks after controlled cortical impact improved hippocampus-dependent spatial and non-spatial cognitive functions in adult C57BL6 mice, as assessed by a battery of neurobehavioral tests for up to 5 weeks after TBI. IL-4-elicited enhancement of cognitive functions was associated with improvements in the integrity of the hippocampus at the functional ( long-term potentiation) and structural levels (CA3 neuronal loss, diffusion tensor imaging of white matter tracts, .). Mechanistically, IL-4 increased the expression of PPARγ and arginase-1 within Mi/MΦ, thereby driving microglia toward a global inflammation-resolving phenotype. Notably, IL-4 failed to shift microglial phenotype after TBI in Mi/MΦ-specific PPARγ knockout (mKO) mice, indicating an obligatory role for PPARγ in IL-4-induced Mi/MΦ polarization. Accordingly, post-TBI treatment with IL-4 failed to improve hippocampal integrity or cognitive functions in PPARγ mKO mice. These results demonstrate that administration of exogenous IL-4 nanoparticles stimulates PPARγ-dependent beneficial Mi/MΦ responses, and improves hippocampal function after TBI.
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http://dx.doi.org/10.1177/0271678X211028680DOI Listing
July 2021

De Novo Design of Peptidic Positive Allosteric Modulators Targeting TRPV1 with Analgesic Effects.

Adv Sci (Weinh) 2021 09 11;8(17):e2101716. Epub 2021 Jul 11.

Kidney Disease Center, First Affiliated Hospital and Department of Biophysics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China.

Transient receptor potential vanilloid 1 (TRPV1) ion channel is a nociceptor critically involved in pain sensation. Direct blockade of TRPV1 exhibits significant analgesic effects but also incurs severe side effects such as hyperthermia, causing failures of TRPV1 inhibitors in clinical trials. In order to selectively target TRPV1 channels that are actively involved in pain-sensing, peptidic positive allosteric modulators (PAMs) based on the high-resolution structure of the TRPV1 intracellular ankyrin-repeat like domain are de novo designed. The hotspot centric approach is optimized for protein design; its usage in Rosetta increases the success rate in protein binder design. It is demonstrated experimentally, with a combination of fluorescence resonance energy transfer (FRET) imaging, surface plasmon resonance, and patch-clamp recording, that the designed PAMs bind to TRPV1 with nanomolar affinity and allosterically enhance its response to ligand activation as it is designed. It is further demonstrated that the designed PAM exhibits long-lasting in vivo analgesic effects in rats without changing their body temperature, suggesting that they have potentials for developing into novel analgesics.
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http://dx.doi.org/10.1002/advs.202101716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425881PMC
September 2021

[Analysis of nine antioxidants in vegetable oils by high performance liquid chromatography].

Se Pu 2020 Jul;38(7):841-846

Chinese Academy of Inspection and Quarantine, Beijing 100176, China.

A method was developed for the determination of nine antioxidants in vegetable oils by high performance liquid chromatography (HPLC). The samples were extracted with methanol, and the fat in the samples was degreased by freezing. Separation of the targeted compounds was performed on an XBridge C column in gradient elution mode using methanol-0.1% (v/v) formic acid aqueous solution as the mobile phase. The analytes were detected using a diode-array detector by the external standard method. The stability and storage conditions for the nine antioxidants were systematically investigated. Ascorbyl palmitate (AP) was introduced into the preparation and pre-treatment of the targets. The concentration of AP was optimized to improve the stability and recovery of the targets. The effects of different extraction solvents and purification methods on the extraction efficiencies were discussed. The results showed that the nine antioxidants could be separated well under the optimized conditions. Good linear relationships in the linear range were obtained, and the correlation coefficients () were greater than 0.999. The average recoveries of the nine antioxidants ranged from 85.3% to 104.1%, with RSDs of the method ≤5.0%. The limits of quantitation (LOQs) for the nine synthetic antioxidants were in the range of 0.6-3.0 mg/kg. The method is simple, rapid, sensitive, and it shows good recovery and reproducibility.
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http://dx.doi.org/10.3724/SP.J.1123.2019.11023DOI Listing
July 2020

Engineering Fe-N Coordination Structures for Fast Redox Conversion in Lithium-Sulfur Batteries.

Adv Mater 2021 Jul 19;33(30):e2100171. Epub 2021 Jun 19.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan, 410083, China.

Critical drawbacks, including sluggish redox kinetics and undesirable shuttling of polysulfides (Li S , n = 4-8), seriously deteriorate the electrochemical performance of high-energy-density lithium-sulfur (Li-S) batteries. Herein, these challenges are addressed by constructing an integrated catalyst with dual active sites, where single-atom (SA)-Fe and polar Fe N are co-embedded in nitrogen-doped graphene (SA-Fe/Fe [email protected]). The SA-Fe, with plane-symmetric Fe-4N coordination, and Fe N, with triangular pyramidal Fe-3N coordination, in this well-designed configuration exhibit synergistic adsorption of polysulfides and catalytic selectivity for Li S lithiation and Li S delithiation, respectively. These characteristics endow the SA-Fe/Fe [email protected] separator with an optimal polysulfides confinement-catalysis ability, thus accelerating the bidirectional liquid-solid conversion (Li S ↔Li S) and suppressing the shuttle effect. Consequently, a Li-S battery based on the SA-Fe/Fe [email protected] separator achieves a high capacity retention of 84.1% over 500 cycles at 1 C (pure S cathode, S content: 70 wt%) and a high areal capacity of 5.02 mAh cm at 0.1 C (SA-Fe/Fe [email protected] S cathode, S loading = 5 mg cm ). It is expected that the outcomes of the present study will facilitate the design of high-efficiency catalysts for long-lasting Li-S batteries.
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http://dx.doi.org/10.1002/adma.202100171DOI Listing
July 2021

A Skull-Removed Chronic Cranial Window for Ultrasound and Photoacoustic Imaging of the Rodent Brain.

Front Neurosci 2021 31;15:673740. Epub 2021 May 31.

Department of Electronic Engineering, Tsinghua University, Beijing, China.

Ultrasound and photoacoustic imaging are emerging as powerful tools to study brain structures and functions. The skull introduces significant distortion and attenuation of the ultrasound signals deteriorating image quality. For biological studies employing rodents, craniotomy is often times performed to enhance image qualities. However, craniotomy is unsuitable for longitudinal studies, where a long-term cranial window is needed to prevent repeated surgeries. Here, we propose a mouse model to eliminate sound blockage by the top portion of the skull, while minimum physiological perturbation to the imaged object is incurred. With the new mouse model, no craniotomy is needed before each imaging experiment. The effectiveness of our method was confirmed by three imaging systems: photoacoustic computed tomography, ultrasound imaging, and photoacoustic mesoscopy. Functional photoacoustic imaging of the mouse brain hemodynamics was also conducted. We expect new applications to be enabled by the new mouse model for photoacoustic and ultrasound imaging.
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http://dx.doi.org/10.3389/fnins.2021.673740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200560PMC
May 2021

Expression, Purification and Characterization of a ZIP Family Transporter from Desulfovibrio vulgaris.

Protein J 2021 Jun 8. Epub 2021 Jun 8.

Department of Geriatrics, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310058, China.

The ZIP family transport zinc ions from the extracellular medium across the plasma membrane or from the intracellular compartments across endomembranes, which play fundamental roles in metal homeostasis and are broadly involved in physiological and pathological processes. Desulfovibrio is the predominant sulphate-reducing bacteria in human colonic microbiota, but also a potential choice for metal bioremediation. while, there are no published studies describing the zinc transporters from Desulfovibrio up to now. In this study, we obtained for the first time a heterologously expressed ZIP homolog from Desulfovibrio vulgaris, termed dvZip. The purified dvZip was reconstituted into proteoliposomes, and confirmed its zinc transport ability in vitro. By combining topology prediction, homology modeling and phylogenetic approaches, we also noticed that dvZip belongs to the GufA and probably have 8 transmembrane α-helical segments (TM 1-8) in which both termini are located on the extracellular, with TM2, 4, 5 and 7 create an inner bundle. We believe that purification and characterization of zinc (probably also cadmium) transporters from Desulfovibrio vulgaris such as dvZip could shed light on understanding of metal homeostasis of Desulfovibrio and provided protein products for future detailed function and structural studies.
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http://dx.doi.org/10.1007/s10930-021-10008-7DOI Listing
June 2021

Photoacoustic Mouse Brain Imaging Using an Optical Fabry-Pérot Interferometric Ultrasound Sensor.

Front Neurosci 2021 17;15:672788. Epub 2021 May 17.

Department of Electronic Engineering, Tsinghua University, Beijing, China.

Photoacoustic (PA, or optoacoustic, OA) mesoscopy is a powerful tool for mouse cerebral imaging, which offers high resolution three-dimensional (3D) images with optical absorption contrast inside the optically turbid brain. The image quality of a PA mesoscope relies on the ultrasonic transducer which detects the PA signals. An all-optical ultrasound sensor based on a Fabry-Pérot (FP) polymer cavity has the following advantages: broadband frequency response, wide angular coverage and small footprint. Here, we present 3D PA mesoscope for mouse brain imaging using such an optical sensor. A heating laser was used to stabilize the sensor's cavity length during the imaging process. To acquire data for a 3D angiogram of the mouse brain, the sensor was mounted on a translation stage and raster scanned. 3D images of the mouse brain vasculature were reconstructed which showed cerebrovascular structure up to a depth of 8 mm with high quality. Imaging segmentation and dual wavelength imaging were performed to demonstrate the potential of the system in preclinical brain research.
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http://dx.doi.org/10.3389/fnins.2021.672788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165253PMC
May 2021

Local electronic structure variation resulting in Li 'filament' formation within solid electrolytes.

Nat Mater 2021 May 31. Epub 2021 May 31.

Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Solid electrolytes hold great promise for enabling the use of Li metal anodes. The main problem is that during cycling, Li can infiltrate along grain boundaries and cause short circuits, resulting in potentially catastrophic battery failure. At present, this phenomenon is not well understood. Here, through electron microscopy measurements on a representative system, LiLaZrO, we discover that Li infiltration in solid oxide electrolytes is strongly associated with local electronic band structure. About half of the LiLaZrO grain boundaries were found to have a reduced bandgap, around 1-3 eV, making them potential channels for leakage current. Instead of combining with electrons at the cathode, Li ions are hence prematurely reduced by electrons at grain boundaries, forming local Li filaments. The eventual interconnection of these filaments results in a short circuit. Our discovery reveals that the grain-boundary electronic conductivity must be a primary concern for optimization in future solid-state battery design.
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http://dx.doi.org/10.1038/s41563-021-01019-xDOI Listing
May 2021

Low-Temperature Selective Catalytic Reduction of NO with NH over Mn-Ce Composites Synthesized by Polymer-Assisted Deposition.

ACS Omega 2021 May 3;6(19):12801-12812. Epub 2021 May 3.

State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.

The Mn Ce binary catalysts with a three-dimensional network structure were successfully prepared via a polymer-assisted deposition method using ethylenediaminetetraacetic acid and polyethyleneimine as complexing agents. The developed pore structure could facilitate the gas diffusion and accelerate the catalytic reaction for NH selective catalytic reduction (SCR). Moreover, the addition of Ce is beneficial for the exposure of active sites on the catalyst surface and increases the adsorption of the NH and NO species. Therefore, the MnCe catalyst exhibits the best catalytic activity for NO removal with a conversion rate of 97% at 180 °C, superior water resistance, and favorable stability. The SCR reaction over the MnCe catalyst takes place through the E-R pathway, which is confirmed by the in situ diffuse reflectance Fourier transform analysis. This work explores a new strategy to fabricate multimetal catalysts and optimize the structure of catalysts.
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http://dx.doi.org/10.1021/acsomega.1c01123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154236PMC
May 2021

A review of pharmacological and pharmacokinetic properties of Forsythiaside A.

Pharmacol Res 2021 Jul 23;169:105690. Epub 2021 May 23.

State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address:

Traditional Chinese medicine plays a significant role in the treatment of various diseases and has attracted increasing attention for clinical applications. Forsythiae Fructus, the dried fruit of Forsythia suspensa (Thunb.) Vahl, is a widely used Chinese medicinal herb in clinic for its extensive pharmacological activities. Forsythiaside A is the main active index component isolated from Forsythiae Fructus and possesses prominent bioactivities. Modern pharmacological studies have confirmed that Forsythiaside A exhibits significant activities in treating various diseases, including inflammation, virus infection, neurodegeneration, oxidative stress, liver injury, and bacterial infection. In this review, the pharmacological activities of Forsythiaside A have been comprehensively reviewed and summarized. According to the data, Forsythiaside A shows remarkable anti-inflammation, antivirus, neuroprotection, antioxidant, hepatoprotection, and antibacterial activities through regulating multiple signaling transduction pathways such as NF-κB, MAPK, JAK/STAT, Nrf2, RLRs, TRAF, TLR7, and ER stress. In addition, the toxicity and pharmacokinetic properties of Forsythiaside A are also discussed in this review, thus providing a solid foundation and evidence for further studies to explore novel effective drugs from Chinese medicine monomers.
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http://dx.doi.org/10.1016/j.phrs.2021.105690DOI Listing
July 2021

Rhodium-Catalyzed Dehydrogenative Cycloisomerization of Dienylcyclopropane to Highly Substituted Toluene.

Org Lett 2021 Jun 24;23(11):4337-4341. Epub 2021 May 24.

Department of Chemistry, Zhejiang University, 20 Yugu Road, Hangzhou 310027, China.

A rhodium-catalyzed dehydrogenative cycloisomerization of dienylcyclopropane compounds is reported, which provides a straightforward approach to a variety of highly substituted toluene derivatives in 67-85% yields. The dienylcyclopropane-imides are produced by a single-step formal three-component olefination procedure. Preliminary mechanistic studies indicated that an electron-withdrawing group as R plays a critical role in completing this transformation.
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http://dx.doi.org/10.1021/acs.orglett.1c01265DOI Listing
June 2021

A Synergistic Coreactant for Single-Cell Electrochemiluminescence Imaging: Guanine-Rich ssDNA-Loaded High-Index Faceted Gold Nanoflowers.

Anal Chem 2021 06 20;93(21):7682-7689. Epub 2021 May 20.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China.

Developing an electrochemiluminescence (ECL) coreactant to minimize the biotoxicity and to maximize the enhancement factor is critical to single-cell ECL microscopy. Here, we reported a guanine-rich single-stranded DNA (G-ssDNA)-loaded high-index faceted gold nanoflower (Hi-AuNF) as a synergistic coreactant of Ru(bpy) for single-cell ECL imaging. Because of the excellent catalytic performance and huge specific surface area, Hi-AuNF serves as not only an ECL enhancer but also a carrier for G-ssDNA. Guanine in G-ssDNA specifically reacts with Ru(bpy) through a so-called "catalytic route" and thus significantly enhances the ECL signal of Ru(bpy). To endow targeting ability to the synergistic coreactant, an aptamer of carcinoembryonic antigen (CEA) is incorporated into the G-ssDNA to form G-ssDNA-Apt for the recognition of human breast adenocarcinoma cells, which overexpress CEA on the cytomembrane. Accordingly, the ECL imaging of CEA on the cytomembrane was realized by using the highly selective [email protected] as the probe as well as the luminophore of Ru(bpy). Compared with the common coreactant tripropylamine with high toxicity and volatility, the [email protected] is considered as a high-performance and biocompatible coreactant, providing exciting opportunities in single-cell imaging and detection.
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http://dx.doi.org/10.1021/acs.analchem.1c00602DOI Listing
June 2021

Sustained bimatoprost release using gold nanoparticles laden contact lenses.

J Biomater Sci Polym Ed 2021 Aug 21;32(12):1618-1634. Epub 2021 Jun 21.

Department of Ophthalmology, The 942 Hospital of Chinese PLA, Yinchuan City, Ningxia Hui Autonomous Region, China.

Contact lenses are ideally suited for sustained ocular drug delivery to bypass the issues associated with eye drop therapy. However, drugs such as bimatoprost loaded by the conventional soaking method show poor drug uptake, high burst release, and altered critical lens properties. In this study, the effect of gold nanoparticles (GNPs) on bimatoprost loading/uptake from the soaking solution and its release kinetics from the lens was investigated. In one method, GNP solutions of varying strength were loaded into the bimatoprost soaking solution (mM-SS batches), and in another method, the GNPs were included in the contact lens matrix during casting (mM-GN-L batches). The GNPs were spherical with average size of 21.1 nm and -20.1 mV zeta potential. The swelling, oxygen permeability, and optical transmittance of the lens were improved compared to those of the lens drug-loaded by the conventional soaking method (SM-L). The mM-GN-L batches showed significant improvement in drug uptake from the soaking solution compared to the SM-L and mM-SS batches. The studies showed relatively low burst and sustained bimatoprost release up to 72 h compared to 24 h with the SM-L batch. The ability to sustain drug release improved proportionally with an increase in the amount of GNPs in the lens. The presence of GNPs lowered protein adherence. The GNP-laden lenses were deemed safe in ocular irritation and histopathology reports (rabbit model). Further, they showed higher drug retention in the rabbit tear fluid compared to the SM-L lens. In conclusion, the presence of GNPs in contact lenses increased drug uptake from the soaking solution, and improved the and release kinetics without affecting the critical properties of the contact lenses for therapeutic application.
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http://dx.doi.org/10.1080/09205063.2021.1927656DOI Listing
August 2021

The Effect of Cochlear Implantation on Tinnitus and Quality of Life: A Systematic Review and Meta-analysis.

Otol Neurotol 2021 Sep;42(8):1113-1122

Department of Otolaryngology-Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina.

Objective: To ascertain the effect of cochlear implantation (CI) on tinnitus and quality of life.

Databases Sources: PubMed, Scopus, Web of Science, and Cochrane Library were searched through August 21, 2020. Search strategies used a combination of subject headings (e.g., MeSH in PubMed) and keywords for the following two concepts: cochlear implantation and tinnitus.

Study Selection: English articles reporting on pre-intervention tinnitus-related patient-reported outcome measures (e.g., Tinnitus Handicap Inventory [THI], Tinnitus Questionnaire [TQ], Visual Analogue Scale [VAS] for loudness) and quality of life measures (e.g., Nijmegen Cochlear Implantation Questionnaire [NCIQ] and Hospital Anxiety and Depression Scale [HADS]) for CI recipients were included.

Data Extraction: Demographics, baseline, and follow-up data.

Data Synthesis: Total of 27 articles reporting on 1,285 patients (mean age 54.5 years, range 14-81) were included. Meta-analysis of all tinnitus-related measures demonstrated improvement following implantation, with a mean difference of -23.2 [95% CI: -28.8 to -17.7], -12.6 [95% CI: -17.5 to -7.8], and -4.5 [95% CI: -5.5 to -3.4] (p < 0.05 for all) for THI, TQ, and VAS, respectively. NCIQ increased by 12.2 points [95% CI: 8.2-16.2] (p < 0.05), indicating improved quality of life among CI recipients. Psychological comorbidities were also ameliorated, as evidenced by reductions in HADS depression (-1.7 [95% CI: -2.4 to -0.9]) and anxiety (-1.3 [95% CI: -2.1 to -0.5]) (p < 0.05 for both) scores.

Conclusion: Following CI, patients reported significant improvement in tinnitus via several validated questionnaires. Additional benefits include improved quality of life and reduction in psychological comorbidities.
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http://dx.doi.org/10.1097/MAO.0000000000003172DOI Listing
September 2021

The Influence of Internet Usage Frequency on Women's Fertility Intentions-The Mediating Effects of Gender Role Attitudes.

Int J Environ Res Public Health 2021 04 30;18(9). Epub 2021 Apr 30.

School of Business, Qingdao University, Qingdao 266061, China.

The purpose of this study is to verify the influence of internet usage frequency on women's fertility intentions and to examine the mediating effects of gender role attitudes, under the influence of internet usage frequency that affects women's fertility intentions, combined with the specific Chinese cultural context. A cross-sectional secondary data analysis was conducted using a sample of 3113 women of childbearing age in the Chinese General Social Survey in 2017 (CGSS2017). The results of the negative binomial regression model showed that, under the premise of controlling individual characteristic variables, the higher the frequency of internet usage, the lower the fertility intention ( < 0.01). The results of the mediating effect model show that the more frequently women use the internet, the lower their fertility intentions, and the less they agree with Chinese traditional gender roles, which are "men work outside to support the family while women stay at home to take care of the family". These findings have implications in formulating public policies aimed at increasing the fertility rate; that is, it is not enough to increase women's fertility intentions under China's universal two-child policy. Moreover, public policy formulators need to consider gender role attitudes and the influence of the internet as a method for dissemination of information.
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http://dx.doi.org/10.3390/ijerph18094784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124929PMC
April 2021

Proteomic Investigation of Glyceraldehyde-Derived Intracellular AGEs and Their Potential Influence on Pancreatic Ductal Cells.

Cells 2021 04 24;10(5). Epub 2021 Apr 24.

The Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.

Glyceraldehyde-derived advanced glycation end products (AGEs) play an important role in the pathogenesis of many diseases including cancer. Accumulation of intracellular AGEs could stimulate cancer induction and facilitate cancer progression. We evaluated the toxic effect of glyceraldehyde-derived intracellular AGEs on normal and malignant pancreatic ductal cells by assessing the cell viability, toxicity, and oxidative stress, followed by proteomic analysis. Our functional studies showed that pancreatic cancer cells (PANC-1 and MIA PaCa-2) were more resistant to glyceraldehyde treatment compared to normal pancreatic ductal epithelial cells (HPDE), while cytotoxicity effects were observed in all cell types. Furthermore, using C isotopic labeled glyceraldehyde, the proteomic data revealed a dose-dependent increment of the number of glycation adducts in both these cell types. HPDE cells showed a higher number of intracellular AGEs compared to cancer cells. At a molecular level, the glycations in the lysine residues of proteins showed a concurrent increase with the concentration of the glyceraldehyde treatment, while the arginine glycations appeared to be less affected by the glyceraldehyde doses. Further pathway analysis of these glycated proteins suggested that the glycated proteins participate in important biological processes that are major hallmarks of cancer initiation and progression, including metabolic processes, immune response, oxidative stress, apoptosis, and S100 protein binding.
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http://dx.doi.org/10.3390/cells10051005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145644PMC
April 2021

Visual recognition of ortho-xylene based on its host-guest crystalline self-assembly with α-cyclodextrin.

J Colloid Interface Sci 2021 Sep 15;597:325-333. Epub 2021 Mar 15.

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China. Electronic address:

Hypothesis: Distinguishing substituted aromatic isomers is a challenging task because of the great similarity of their physicochemical properties. Considering xylene isomers have drastically different geometrical shapes, we predict this would show great impact on the self-assembling behavior of various xylene [email protected] inclusion complex.

Experiments: Through host-guest crystalline self-assembly, among three isomers, only ortho-xylene is capable to form hydrogels with α-cyclodextrin. ROESY NMR, molecular simulations and circular dichroism spectra suggest that the ortho selectivity comes from the difference in the conformation of host-guest building block. The larger volume, and steric hinderance of the ortho isomer make it most possibly decrease their tendency to adopt more mobile orientations in cyclodextrin-based complex as meta and para isomers do, resulting in gel formation.

Findings: Herein, we report a novel, facile and environmentally-friendly protocol on the recognition of ortho benzene isomers using α-cyclodextrin through host-guest crystalline self-assembly. Visual recognition of ortho-xylene is achieved through amplifying the structural difference of xylene isomers at molecular scale into macroscopic scale. We believe this work unveils subtle rules to control macroscopic assemblies at the molecular level and highlights the potential of using macrocyclic compounds to improve the quality and reduce the energy bill for separation in petrochemical industry.
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http://dx.doi.org/10.1016/j.jcis.2021.03.024DOI Listing
September 2021

Bright and Near-Unity Polarized Light Emission Enabled by Highly Luminescent CuI-Dimer Cluster-Based Hybrid Materials.

Nano Lett 2021 May 22;21(9):4115-4121. Epub 2021 Apr 22.

Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China.

As one fundamental property of light, polarization has a huge impact in quantum optics and optoelectronics through light-matter interactions. However, the bright and near-unity polarized light emissions in the visible range by solid crystalline materials are scantly realized. Here, we report well-defined quasi two-dimensional (2D) hybrid crystals based on the linear alignment of CuI-dimer/bidentate ligand hybrid clusters for achieving bright and near-unity linearly polarized light emissions. Using first-principle calculations, we demonstrate that the superaligned transition dipole moments are the key for the observed excellent polarized light emissions. To further enhance the photoluminescence (PL) polarization degree, we fabricate CuI-dimer-based hybrid nanobelts, which display high PL quantum yield (up to 64%) and ultrahigh PL polarization degree (∼0.99). Our reported copper iodine cluster-based luminescent hybrid materials for bright and highly polarized light emissions will have great potential for future quantum optics applications.
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http://dx.doi.org/10.1021/acs.nanolett.1c01149DOI Listing
May 2021

Deep learning in photoacoustic imaging: a review.

J Biomed Opt 2021 04;26(4)

Tsinghua University, Department of Electronic Engineering, Haidian, Beijing, China.

Significance: Photoacoustic (PA) imaging can provide structural, functional, and molecular information for preclinical and clinical studies. For PA imaging (PAI), non-ideal signal detection deteriorates image quality, and quantitative PAI (QPAI) remains challenging due to the unknown light fluence spectra in deep tissue. In recent years, deep learning (DL) has shown outstanding performance when implemented in PAI, with applications in image reconstruction, quantification, and understanding.

Aim: We provide (i) a comprehensive overview of the DL techniques that have been applied in PAI, (ii) references for designing DL models for various PAI tasks, and (iii) a summary of the future challenges and opportunities.

Approach: Papers published before November 2020 in the area of applying DL in PAI were reviewed. We categorized them into three types: image understanding, reconstruction of the initial pressure distribution, and QPAI.

Results: When applied in PAI, DL can effectively process images, improve reconstruction quality, fuse information, and assist quantitative analysis.

Conclusion: DL has become a powerful tool in PAI. With the development of DL theory and technology, it will continue to boost the performance and facilitate the clinical translation of PAI.
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http://dx.doi.org/10.1117/1.JBO.26.4.040901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033250PMC
April 2021

Predictive factors for spontaneous stone passage in diabetic patients with acute ureteric colic.

Chin Med J (Engl) 2021 Apr 1. Epub 2021 Apr 1.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, Cleveland, OH, USA Department of Urology, University of California, San Diego, CA, USA.

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http://dx.doi.org/10.1097/CM9.0000000000001456DOI Listing
April 2021

Development of a digital breast phantom for photoacoustic computed tomography.

Biomed Opt Express 2021 Mar 10;12(3):1391-1406. Epub 2021 Feb 10.

The Department of Electronic Engineering, Tsinghua University, Beijing, 100084, China.

Photoacoustic (PA) imaging provides morphological and functional information about angiogenesis and thus is potentially suitable for breast cancer diagnosis. However, the development of PA breast imaging has been hindered by inadequate patients and a lack of ground truth images. Here, we report a digital breast phantom with realistic acoustic and optical properties, with which a digital PA-ultrasound imaging pipeline is developed to create a diverse pool of virtual patients with three types of masses: ductal carcinoma in situ, invasive breast cancer, and fibroadenoma. The experimental results demonstrate that our model is realistic, flexible, and can be potentially useful for accelerating the development of PA breast imaging technology.
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http://dx.doi.org/10.1364/BOE.416406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984796PMC
March 2021
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