Publications by authors named "Cheng Li"

2,500 Publications

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A reference-grade genome assembly for Gossypium bickii and insights into its genome evolution and formation of pigment gland and gossypol.

Plant Commun 2022 Aug 9:100421. Epub 2022 Aug 9.

Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China; Hainan Institute, Zhejiang University, Sanya, China. Electronic address:

Pigment gland is a morphological characteristic of Gossypium and its related genera. Gossypium bickii (G) is characterized by delayed pigment gland morphogenesis in the cotyledons. In this study, a reference-grade genome of G was generated, and comparative genomics analysis showed that G was closest to Gossypium australe (G), followed by A and D-genome species. Two large fragment-translocations in chromosomes 5 and 13 were detected between the G-genome and other Gossypium genomes, which were unique to the G and G-genomes. Compared to the G-genome, two large fragment-inversions in the chromosomes 12 and 13 were detected in G. According to the phylogeny, divergence time, and similarity analysis of nuclear and chloroplast genomes, G was formed by hybridization between Gossypium sturtianum (C) and a common ancestor of G and Gossypium nelsonii (G). The coordinated expression pattern of pigment gland formation (GoPGF) and gossypol biosynthesis genes in G. bickii was verified to be consistent with its phenotype, and nine genes that were related to the process of pigment gland formation were identified. A novel gene, GbiCYP76B6 regulated by GoPGF was found to affect gossypol biosynthesis. These findings offer insights into the origin and evolution of G, and its mechanism of pigment gland formation and gossypol biosynthesis.
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http://dx.doi.org/10.1016/j.xplc.2022.100421DOI Listing
August 2022

Letter to the Editor Regarding "Preemptive Intravenous Nalbuphine for the Treatment of Post-Operative Visceral Pain: A Multicenter, Double-Blind, Placebo-Controlled, Randomized Clinical Trial".

Pain Ther 2022 Aug 9. Epub 2022 Aug 9.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, NO. 95 Yong-An Road, Xi-Cheng District, Beijing, 100050, People's Republic of China.

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http://dx.doi.org/10.1007/s40122-022-00414-9DOI Listing
August 2022

Severe epilepsy phenotype with SCN1A missense variants located outside the sodium channel core region: Relationship between functional results and clinical phenotype.

Seizure 2022 Aug 3;101:109-116. Epub 2022 Aug 3.

Department of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, No. 136, Zhongshan Er Road, Yuzhong District, Chongqing 400014, China. Electronic address:

Purpose: Most SCN1A missense variants located outside the sodium channel core region show a mild phenotype. However, there are exceptions, because of which it is challenging to determine the correlation between genotype and phenotype. In this study, we aimed to determine whether functional study could be used to determine disease severity in cases with such variants, and elucidate possible genotype-phenotype relationships.

Methods: Forty-seven patients with SCN1A missense variants were recruited, and one with a Dravet syndrome phenotype with an SCN1A missense variant (c.3811T>C/ p.W1271R) located outside the core region was screened with electrophysiological tests. We also reviewed functional SCN1A studies on patients with inconsistent phenotypes and genotypes, and studied the relationship between electrophysiological measurements and clinical phenotype.

Results: Patch clamp experiments showed that the W1271R variant caused significantly reduced sodium current, decreased channel voltage sensitivity, loss of channel availability, and prolonged recovery time from inactivation compared with wild type (WT), which ultimately caused a change in loss of function (LOF). Twelve cases of severe SCN1A-related epilepsy with missense variants located outside the channel core region were also included from the functional studies. Nine patients with missense SCN1A variants showed complete (3/9) or partial (6/9) physiological LOF. Two missense SCN1A variants caused physiological gain-and-loss of function (G-LOF), and one caused decreased excitability (DE).

Conclusions: Not all missense variants located outside the core region cause a mild phenotype. Although current functional studies in heterologous expression systems do not accurately reflect disease severity caused by SCN1A missense variants, they could be an effective model for generation of data to study the initial effects of SCN1A missense variants.
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http://dx.doi.org/10.1016/j.seizure.2022.07.018DOI Listing
August 2022

Managing Methane Emissions in Abandoned Coal Mines: Comparison of Different Recovery Technologies by Integrating Techno-Economic Analysis and Life-Cycle Assessment.

Environ Sci Technol 2022 Aug 9. Epub 2022 Aug 9.

School of Environment, Tsinghua University, Beijing 100084, China.

Methane emissions from worldwide increasing abandoned coal mines have posed multiple challenges of global warming, energy waste, and explosion risk. This study first profiles the dynamic patterns of coal mine methane emissions in different recovery technologies, methane extraction with drainage (MEWD, mine-water concurrently extracted and treated) and direct methane extraction (DME, noncontrol on mine-water), in two abandoned mines from Ningxia and Inner Mongolia as China's leading coal provinces. Then, we conducted a techno-economic analysis and life-cycle assessment to quantify their comprehensive benefits. The key findings are as follows: (1) MEWD can long recover methane, although the economic profits decrease with declining methane extraction volume. DME can only work for ∼5 years, after which the mine is flooded, where methane is sealed underground and not recoverable. (2) MEWD drains and further treats the mine-water with an additional 29.4-35.9 million CNY cost compared with DME, while MEWD can achieve greater life-cycle environmental benefits with more cumulative methane recovery, whose CO-eq (GWP-100) and SO reductions are 64.4 and 53.4% higher than those of DME. (3) MEWD is more promising for large-scale implementation, where feed-in tariffs and carbon market measures can improve the economics for sustainable management of incremental abandoned mine methane.
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http://dx.doi.org/10.1021/acs.est.2c02928DOI Listing
August 2022

High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis.

Biochem Genet 2022 Aug 9. Epub 2022 Aug 9.

Department of Radiotherapy, Shaanxi Provincial People's Hospital, No. 256 Youyi West Rd, Xi'an, 710068, Shaanxi, China.

It has been reported that circRNAs play an important regulatory role in the progression of colorectal cancer (CRC). However, the molecular role of circ_0001821 in CRC development is unclear. In this study, we aimed to investigate the regulatory role and molecular mechanisms of circ_0001821 in CRC. Reverse transcription-quantitative PCR and western blot assays were used to detect the expression of circ_0001821, miR-600 and isochorismatase domain containing 1 (ISOC1) in CRC tissues as well as its cell lines. Colony formation assay and EDU assay were used to detect the proliferative capacity of cells. Transwell assay was used to assess cell migration and invasion ability. Flow cytometry was used to analyze cell apoptosis. ELISA was used to measure the glycolytic capacity of cells. Dual-luciferase reporter assay and RNA pull-down assay were used to analyze the relationships between circ_0001821, miR-600 and ISOC1. Animal experimentation was used to validate the functional study of circ_0001821 in vivo. Immunohistochemistry (IHC) of Ki67 staining analysis was conducted to assess tumor growth. Circ_0001821 and ISOC1 were significantly increased in CRC tissues and its cell lines, and miR-600 was significantly decreased in CRC tissues and its cell lines. Silencing circ_0001821 inhibited cell proliferation, migration, invasion and glycolytic capacity, while inducing apoptosis. And it could inhibit tumor growth in vivo. Circ_0001821 could act as a sponge for miR-600 to regulate CRC processes. ISOC1 was identified as a downstream regulator of miR-600, also miR-600 could regulate the expression of ISOC1. In addition, circ_0001821 could regulate ISOC1 expression changes through miR-600. Mechanistically, either miR-600 inhibitor or overexpression of ISOC1 could reverse the effects of knockdown of circ_0001821 on cell biological properties. Circ_0001821 regulated the developmental process of CRC through miR-600/ISOC1 axis.
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http://dx.doi.org/10.1007/s10528-022-10262-zDOI Listing
August 2022

A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments.

Nat Commun 2022 Aug 8;13(1):4614. Epub 2022 Aug 8.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Single-chain variable fragments (scFvs), composed of variable domains of heavy and light chains of an antibody joined by a linker, share antigen binding capacity with their parental antibody. Due to intrinsically low solubility and stability, only two Escherichia coli-produced scFvs have been approved for therapy. Here we report that a 33-residue peptide, termed P17 tag, increases the solubility of multiple scFvs produced in Escherichia coli SHuffle strain by up to 11.6 fold. Hydrophilic sequence, especially charged residues, but not the predicted α-helical secondary structure of P17 tag, contribute to the solubility enhancement. Notably, the P17 tag elevates the thermostability of scFv as efficiently as intra-domain disulfide bonds. Moreover, a P17-tagged scFv targeting hepatitis B virus surface proteins shows over two-fold higher antigen-binding affinity and virus-neutralizing activity than the untagged version. These data strongly suggest a type I intramolecular chaperone-like activity of the P17 tag. Hence, the P17 tag could benefit the research, production, and application of scFv.
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http://dx.doi.org/10.1038/s41467-022-32423-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359998PMC
August 2022

Observation of a State X(2600) in the π^{+}π^{-}η' System in the Process J/ψ→γπ^{+}π^{-}η'.

Phys Rev Lett 2022 Jul;129(4):042001

Institute of High Energy Physics, Beijing 100049, People's Republic of China.

Based on (10087±44)×10^{6}  J/ψ events collected with the BESIII detector, the process J/ψ→γπ^{+}π^{-}η^{'} is studied using two largest decay channels of the η^{'} meson, η^{'}→γπ^{+}π^{-} and η^{'}→ηπ^{+}π^{-}, η→γγ. A new resonance, which we denote as the X(2600), is observed with a statistical significance larger than 20σ in the π^{+}π^{-}η^{'} invariant mass spectrum, and it has a connection to a structure around 1.5  GeV/c^{2} in the π^{+}π^{-} invariant mass spectrum. A simultaneous fit on the π^{+}π^{-}η^{'} and π^{+}π^{-} invariant mass spectra with the two η^{'} decay modes indicates that the mass and width of the X(2600) state are 2618.3±2.0_{-1.4}^{+16.3}  MeV/c^{2} and 195±5_{-17}^{+26}  MeV, where the first uncertainties are statistical, and the second systematic.
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http://dx.doi.org/10.1103/PhysRevLett.129.042001DOI Listing
July 2022

Feasibility and safety of next-day discharge following transcatheter bicuspid aortic valve replacement.

Int J Cardiol Heart Vasc 2022 Oct 2;42:101101. Epub 2022 Aug 2.

Department of Cardiology, Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

Background: Decreased length of stay in the index hospitalization is a tendency in transcatheter aortic valve replacement (TAVR) era. In this study, we aim to evaluate the feasibility and safety of next-day discharge (NDD) in bicuspid aortic valve (BAV) patients following TAVR.

Methods: The study analyzed patients who received TAVR in 2019 to 2022. Thirty-day mortality and readmission rate were compared between BAV and tricuspid aortic valve (TAV) patients.

Results: The proportion of NDD was similar between the BAV and TAV group (45.3 % vs 41.3 %, p = 0.487). In NDD patients, the lower age (72.0 [67.0, 77.0] yrs vs 74.0 [70.0, 80.0] yrs, p = 0.011) and STS score (2.33 [1.56, 3.54] % vs 3.82 [2.38, 5.70] %, p < 0.001) were observed in the BAV group. The NDD BAV patients had higher proportion of post-dilatation (74.3 % vs 50.7 %, p = 0.003) when compared with the TAV patients. The NDD patients was safe with no death both in BAV and TAV patients at 30-day follow-up. Moreover, the readmission rate was comparable between BAV and TAV patients who discharged on the next day after TAVR (8.1 % vs 14.0 %, p = 0.397).

Conclusions: NDD after TAVR was feasible and safe in both BAV and TAV patients. The younger BAV patients with fast recovery deserve the next-day discharge after TAVR.
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http://dx.doi.org/10.1016/j.ijcha.2022.101101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350863PMC
October 2022

Nanoantidote for repression of acidosis pH promoting COVID-19 infection.

View (Beijing) 2022 Jul 1;3(4):20220004. Epub 2022 May 1.

Department of Anesthesiology and Perioperative Medicine Shanghai Fourth People's Hospital, School of Medicine, Tongji University Shanghai P. R. China.

Acidosis, such as respiratory acidosis and metabolic acidosis, can be induced by coronavirus disease 2019 (COVID-19) infection and is associated with increased mortality in critically ill COVID-19 patients. It remains unclear whether acidosis further promotes SARS-CoV-2 infection in patients, making virus removal difficult. For antacid therapy, sodium bicarbonate poses great risks caused by sodium overload, bicarbonate side effects, and hypocalcemia. Therefore, new antacid antidote is urgently needed. Our study showed that an acidosis-related pH of 6.8 increases SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) expression on the cell membrane by regulating intracellular microfilament polymerization, promoting SARS-CoV-2 pseudovirus infection. Based on this, we synthesized polyglutamic acid-PEG materials, used complexation of calcium ions and carboxyl groups to form the core, and adopted biomineralization methods to form a calcium carbonate nanoparticles (CaCO-NPs) nanoantidote to neutralize excess hydrogen ions (H), and restored the pH from 6.8 to approximately 7.4 (normal blood pH). CaCO-NPs effectively prevented the heightened SARS-CoV-2 infection efficiency due to pH 6.8. Our study reveals that acidosis-related pH promotes SARS-CoV-2 infection, which suggests the existence of a positive feedback loop in which SARS-CoV-2 infection-induced acidosis enhances SARS-CoV-2 infection. Therefore, antacid therapy for acidosis COVID-19 patients is necessary. CaCO-NPs may become an effective antacid nanoantidote superior to sodium bicarbonate.
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http://dx.doi.org/10.1002/VIW.20220004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347551PMC
July 2022

Therapeutic Effect of Ultrasound Combined With Porous Lipid Clioquinol/PLGA Microbubbles on Ferroptosis in HL-1 Cardiac Cell Induced by Isoproterenol Attack.

Front Pharmacol 2022 22;13:918292. Epub 2022 Jul 22.

Henan Key Laboratory of Medical Tissue Regeneration, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.

In recent years, studies have shown a close relationship between cardiomyocyte death and ferroptosis. Clioquinol (CQ) can inhibit ferroptosis. Porous lipid-poly (lactic-co-glycolic acid) (PLGA) microbubbles (MBs) were prepared by double emulsification (W/O/W) using 1,2-dioctadecanoyl-sn-glycero-3-phophocholine and PLGA as raw materials. Porous lipid-PLGA MBs were used as carriers to prepare CQ/PLGA MBs containing CQ. CQ/PLGA had the advantages of high drug loading, good biocompatibility, and sustained release. Our results showed that CQ/PLGA improved the effect of CQ and reduced its cytotoxicity. Under low-frequency ultrasound with certain parameters, CQ/PLGA showed steady-state cavitation, which increased the membrane permeability of mouse cardiomyocyte HL-1 to a certain extent and further prevented the process of ferroptosis in mouse cardiomyocyte HL-1.
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http://dx.doi.org/10.3389/fphar.2022.918292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354950PMC
July 2022

Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa.

Plant Methods 2022 Aug 6;18(1):98. Epub 2022 Aug 6.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.

Background: Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency.

Results: In this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number.

Conclusion: The results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip.
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http://dx.doi.org/10.1186/s13007-022-00931-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356411PMC
August 2022

Effects of beeswax emulsified by octenyl succinate starch on the structure and physicochemical properties of acid-modified starchfilms.

Int J Biol Macromol 2022 Aug 2;219:262-272. Epub 2022 Aug 2.

College of Food Science and Engineering, Shandong Agricultural University, Engineering and Technology Center for Grain Processing of Shandong Province, Tai'an, PR China. Electronic address:

This work aimed to develop a novel strategy to modulate the distribution of beeswax in acid-modified starch films via tuning octenyl succinate starch (OSS) ratios and to elucidate their structure-property relationships. The apparent viscosity and storage modulus of the film-forming solution decreased with the increase of OSS ratio. Attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy revealed that the hydrogen bond in the film-forming network was cleaved with the presence of OSS. Scanning electron microscope (SEM), atomic force microscope (AFM), and X-ray diffraction (XRD) demonstrated that OSS ratio had an obvious effect on the formation and distribution of beeswax crystal particles. Uniform distribution of beeswax effectively enhanced the hydrophobicity and water barrier properties of films and performed preferable elongation at break but at the expense of tensile strength and optical properties. The films with higher OSS ratio (>12 %) presented higher thermal stability. This study provides new information on the rational design of emulsified films to obtain desirable physicochemical properties by tuning the distribution of beeswax.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.235DOI Listing
August 2022

Antepartum sleep quality, mental status, and postpartum depressive symptoms: a mediation analysis.

BMC Psychiatry 2022 08 2;22(1):521. Epub 2022 Aug 2.

Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, 200011, China.

Background: Poor sleep quality and maternal mood disturbances are common during pregnancy and may play pivotal roles in the development of postpartum depression. We aim to examine the trajectories of sleep quality and mental health in women from early pregnancy to delivery and explore the mediating effects of sleep quality and mental status on the link between antepartum depressive symptoms and postpartum depressive symptoms.

Methods: In an ongoing prospective birth cohort, 1301 women completed questionnaires in the first, second and third trimesters and at 6 weeks postpartum. In each trimester, sleep quality was measured utilizing the Pittsburgh Sleep Quality Index (PSQI), and mental health was assessed with the Center for Epidemiologic Studies Depression Scale (CES-D), the Self-Rating Anxiety Scale (SAS) and the Perceived Stress Scale (PSS). Postpartum depressive symptoms were evaluated by the Edinburgh Postnatal Depression Scale (EPDS). The bootstrap method was used to test the mediation effect.

Results: The PSQI, CES-D, and SAS scores presented U-shaped curves across the antenatal period while the PSS score followed a descending trend. Antenatal sleep quality, depressive symptoms, anxiety symptoms and perceived stress all predicted depressive symptoms at 6 weeks postpartum. The influence of antepartum depressive symptoms on postpartum depressive symptoms was mediated by antepartum sleep quality and anxiety symptoms, which accounted for 32.14%, 39.25% and 31.25% in the first, second and third trimesters (P = 0.002, P = 0.001, P = 0.001, respectively).

Conclusions: Poor sleep quality and anxiety symptoms in pregnancy mediated the relationship between antepartum depressive symptoms and postpartum depressive symptoms. Interventions aimed at detecting and managing sleep quality and elevated anxiety among depressed women in pregnancy warrant further investigation as preventative strategies for postpartum depression.
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http://dx.doi.org/10.1186/s12888-022-04164-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344627PMC
August 2022

A novel steady-state model to quantitively assess the effect of pH elevation by dissimilatory sulfate reduction process in acidic waters in mining areas.

Water Res 2022 Jul 12;222:118852. Epub 2022 Jul 12.

Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, School of Environmental Science & Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Acidic waters such as groundwater, drainage and lakes in mining area contain high-strength acids and metal ions, posing serious threats to aquatic ecosystems and human health. Dissimilatory sulfate reduction (DSR)-based processes are attractive technologies for remediating acidic waters because it produces alkalinity and sulfide for metal precipitation and acid neutralization. However, the effects of pH elevation achieved by DSR-based processes are case-sensitive and difficult to be quantitively assessed, which limits the application of DSR process for acidic water remediation. Therefore, in this study, a Sulfidogenic Acid mine water Remediation Model (SARM) considering the DSR process, weak acids balance, metal sulfide and hydroxide precipitations, and gas-liquid exchanges of HS and CO, was developed to quantitatively assess the effects of various environmental factors on the pH elevation by a DSR process in acidic waters. A long-term trial of a DSR reactor was conducted to calibrate and validate the SARM. The experimental results revealed that the DSR-based process is effective to relieve acidity. The calibrated SARM demonstrated the excellent performance to predict the pH variation in the DSR reactor, under the varied conditions of influent pH and organic concentration. The calibrated SARM was further validated with data collected from literatures, and the results verified that the proposed model is capable to accurately assess the effect of DSR process on acid neutralization and metal removals under various conditions in steady state. The model was employed to systematically evaluate the impacts of environmental factors on acid remediation within a DSR-based process. The results revealed that the background alkalinity plays an important role in acid neutralization. However, with an increase in sulfate reduction, biogenic sulfide and carbonate become the dominant buffering substances to neutralize acidity. Furthermore, the SARM was used to evaluate the applicability of the DSR-based process for the remediation of acidic waters by evaluating the sulfide production thresholds for acid neutralization and metal removal. The simulation results demonstrated that, the DSR-based process is recommended for the remediation of acidic waters with low background alkalinity. Collectively, the SARM proposed in this study was found to be a useful and efficient tool for quantitatively assessing the potential of DSR-based processes for neutralizing acidic waters, which is vital for biogeochemistry and environmental engineering research.
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http://dx.doi.org/10.1016/j.watres.2022.118852DOI Listing
July 2022

Selective Overview of 3D Heterogeneity in CMOS.

Nanomaterials (Basel) 2022 Jul 8;12(14). Epub 2022 Jul 8.

Department of Electrical and Computer Engineering, University of California, Riverside, CA 92521, USA.

As the demands for improved performance of integrated circuit (IC) chips continue to increase, while technology scaling driven by Moore's law is becoming extremely challenging, if not impractical or impossible, heterogeneous integration (HI) emerges as an attractive pathway to further enhance performance of Si-based complementary metal-oxide-semiconductor (CMOS) chips. The underlying basis for using HI technologies and structures is that IC performance goes well beyond classic logic functions; rather, functionalities and complexity of smart chips span across the full information chain, including signal sensing, conditioning, processing, storage, computing, communication, control, and actuation, which are required to facilitate comprehensive human-world interactions. Therefore, HI technologies can bring in more function diversifications to make system chips smarter within acceptable design constraints, including costs. Over the past two decades or so, a large number of HI technologies have been explored to increase heterogeneities in materials, technologies, devices, circuits, and system architectures, making it practically impossible to provide one single comprehensive review of everything in the field in one paper. This article chooses to offer a topical overview of selected HI structures that have been validated in CMOS platforms, including a stacked-via vertical magnetic-cored inductor structure in CMOSs, a metal wall structure in the back end of line (BEOL) of CMOSs to suppress global flying noises, an above-IC graphene nano-electromechanical system (NEMS) switch and nano-crossbar array electrostatic discharge (ESD) protection structure, and graphene ESD interconnects.
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http://dx.doi.org/10.3390/nano12142340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322364PMC
July 2022

Progress and prospects of modified starch-based carriers in anticancer drug delivery.

J Control Release 2022 Jul 28;349:662-678. Epub 2022 Jul 28.

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, People's Republic of China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China; Collaborative Innovation Center for Food Safety and Quality Control, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China. Electronic address:

Recently, the role of starch-based carrier systems in anticancer drug delivery has gained considerable attention. Although there are same anticancer drugs, difference in their formulations account for unique therapeutic effects. However, the exploration on the effect-enhancing of anticancer drugs and their loading system by modified starch from the perspective of carrier regulation is still limited. Moreover, research on the reduced toxicity of the anticancer drugs due to modified starch as the drug carrier mediated by the intestinal microenvironment is lacking, but worth exploring. In this review, we examined the effect of modified starch on the loading and release properties of anticancer drugs, and the effect of resistant starch and its metabolites on intestinal microecology during inflammation. Particularly, the interactions between modified starch and drugs, and the effect of resistant starch on gene expression, protein secretion, and inflammatory factors were discussed. The findings of this review could serve as reference for the development of anticancer drug carriers in the future.
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http://dx.doi.org/10.1016/j.jconrel.2022.07.024DOI Listing
July 2022

Phenotypic and Transcriptomics Analyses Reveal Underlying Mechanisms in a Mouse Model of Corneal Bee Sting.

Toxins (Basel) 2022 Jul 8;14(7). Epub 2022 Jul 8.

Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Xiamen 361102, China.

Corneal bee sting (CBS) is one of the most common ocular traumas and can lead to blindness. The ophthalmic manifestations are caused by direct mechanical effects of bee stings, toxic effects, and host immune responses to bee venom (BV); however, the underlying pathogenesis remains unclear. Clinically, topical steroids and antibiotics are routinely used to treat CBS patients but the specific drug targets are unknown; therefore, it is imperative to study the pathological characteristics, injury mechanisms, and therapeutic targets involved in CBS. In the present study, a CBS injury model was successfully established by injecting BV into the corneal stroma of healthy C57BL/6 mice. F-actin staining revealed corneal endothelial cell damage, decreased density, skeletal disorder, and thickened corneal stromal. The terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) assay showed apoptosis of both epithelial and endothelial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that cytokine-cytokine interactions were the most relevant pathway for pathogenesis. Protein-protein interaction (PPI) network analysis showed that IL-1, TNF, and IL-6 were the most relevant nodes. RNA-seq after the application of Tobradex (0.3% tobramycin and 0.1% dexamethasone) eye ointment showed that Tobradex not only downregulated relevant inflammatory factors but also reduced corneal pain as well as promoted nerve regeneration by repairing axons. Here, a stable and reliable model of CBS injury was successfully established for the first time, and the pathogenesis of CBS and the therapeutic targets of Tobradex are discussed. These hub genes are expected to be biomarkers and therapeutic targets for the diagnosis and treatment of CBS.
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http://dx.doi.org/10.3390/toxins14070468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323056PMC
July 2022

Enhancing Drug Utilization Efficiency Dish-Structured Triboelectric Nanogenerator.

Front Bioeng Biotechnol 2022 6;10:950146. Epub 2022 Jul 6.

School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Due to the finding of severe side effects and low therapeutic efficacy with cancer chemotherapy, there still remains a great challenge to benefit patients with curative effect. In this work, we designed a self-powered drug delivery system comprising a current source derived from the disk TENG (D-TENG) and a pair of Au electrodes. Thus, cells seeded within the electrode gap could be stimulated by the current followed by D-TENG`s work. Under the rotation frequency of about 7.4 Hz, the peak output current and voltage of the D-TENG reached 3.7 μA and 135 V and achieved an average of 2.8 μA of output current. Furthermore, the D-TENG also showed its good stability to output steady current in a long-term condition. When applying the electric stimulation by this self-powered drug delivery system, a chemotherapy drug, doxorubicin (DOX), had significant uptake by cancer cells. Therefore, utilizing a novel TENG device as a part of chemotherapy would provide a new opportunity in future disease treatment.
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http://dx.doi.org/10.3389/fbioe.2022.950146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298755PMC
July 2022

The degree of substitution of OSA-modified starch affects the retention and release of encapsulated mint flavour.

Carbohydr Polym 2022 Oct 27;294:119781. Epub 2022 Jun 27.

School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, Jiangsu Province, China. Electronic address:

This study explored the effect of the degree of substitution (DS) of octenyl succinic anhydride (OSA)-modified starch on the retention and release properties of spray-dried mint flavour microcapsules. The results showed that a higher DS (0.0287, 0.0379) led to greater flavour retention (86.68 %, 90.10 %). The flavour release was negatively correlated with DS during simulated oral processing. A mechanism by which DS affects flavour release was proposed. The emulsifying capacity was improved with increasing DS, thus forming an effective interface. The effective interface increased the partition coefficient, thereby reducing the release. During oral processing, a lower friction coefficient and higher microstructural stability and carrier enzymatic resistance jointly promoted interface stabilization. Data obtained using the EPI suite, a gas chromatograph, a mini traction machine, a Mastersizer analyser and confocal scanning laser microscopy validated this mechanism. This study provides a strategy for designing flavour microcapsules with high retention and slow-release properties using OSA-modified starch.
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http://dx.doi.org/10.1016/j.carbpol.2022.119781DOI Listing
October 2022

Observation of J/ψ Electromagnetic Dalitz Decays to X(1835), X(2120), and X(2370).

Phys Rev Lett 2022 Jul;129(2):022002

Turkish Accelerator Center Particle Factory Group, Istanbul Bilgi University, 34060 Eyup, Istanbul, Turkey.

Using a sample of about 10^{10}  J/ψ events collected at a center-of-mass energy sqrt[s]=3.097  GeV with the BESIII detector, the electromagnetic Dalitz decays J/ψ→e^{+}e^{-}π^{+}π^{-}η^{'}, with η^{'}→γπ^{+}π^{-} and η^{'}→π^{+}π^{-}η, have been studied. The decay J/ψ→e^{+}e^{-}X(1835) is observed with a significance of 15σ, and also an e^{+}e^{-} invariant-mass dependent transition form factor of J/ψ→e^{+}e^{-}X(1835) is presented for the first time. The intermediate states X(2120) and X(2370) are also observed in the π^{+}π^{-}η^{'} invariant-mass spectrum with significances of 5.3σ and 7.3σ. The corresponding product branching fractions for J/ψ→e^{+}e^{-}X, X→π^{+}π^{-}η^{'} [X=X(1835), X(2120), and X(2370)] are reported.
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http://dx.doi.org/10.1103/PhysRevLett.129.022002DOI Listing
July 2022

DPProm: A Two-layer Predictor for Identifying Promoters and Their Types on Phage Genome Using Deep Learning.

IEEE J Biomed Health Inform 2022 Jul 22;PP. Epub 2022 Jul 22.

With the number of phage genomes increasing, it is urgent to develop new bioinformatics methods for phage genome annotation. Promoter, a DNA region, is important for gene transcriptional regulation. In the era of post-genomics, the availability of data makes it possible to establish computational models for promoter identification with robustness. In this work, we introduce DPProm, a two-layer model composed of DPProm-1L and DPProm-2L, to predict promoters and their types for phages. On the first layer, as a dual-channel deep neural network ensemble method fusing multi-view features (sequence feature and handcrafted feature), the model DPProm-1L is proposed to identify whether a DNA sequence is a promoter or non-promoter. The sequence feature is extracted with convolutional neural network (CNN). And the handcrafted feature is the combination of free energy, GC content, cumulative skew, and Z curve features. On the second layer, DPProm-2L based on CNN is trained to predict the promoters' types (host or phage). For the realization of prediction on the whole genomes, the model DPProm, combines with a novel sequence data processing workflow, which contains sliding window and merging sequences modules. Experimental results show that DPProm outperforms the state-of-the-art methods, and decreases the false positive rate effectively on whole genome prediction. Furthermore, we provide a user-friendly web at http://bioinfo.ahu.edu.cn/DPProm. We expect that DPProm can serve as a useful tool for identification of promoters and their types.
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http://dx.doi.org/10.1109/JBHI.2022.3193224DOI Listing
July 2022

Short and long-term effects of robot-assisted therapy on upper limb motor function and activity of daily living in patients post-stroke: a meta-analysis of randomized controlled trials.

J Neuroeng Rehabil 2022 Jul 21;19(1):76. Epub 2022 Jul 21.

Department of Health Management, The Second Affiliated Hospital of Chongqing Medical University, 76 Linjiang Road, Yuzhong, Chongqing, 400010, China.

Objective: To investigate the effect of robot-assisted therapy (RAT) on upper limb motor control and activity function in poststroke patients compared with that of non-robotic therapy.

Methods: We searched PubMed, EMBASE, Cochrane Library, Google Scholar and Scopus. Randomized controlled trials published from 2010 to nowadays comparing the effect of RAT and control treatment on upper limb function of poststroke patients aged 18 or older were included. Researchers extracted all relevant data from the included studies, assessed the heterogeneity with inconsistency statistics (I statistics), evaluated the risk of bias of individual studies and performed data analysis.

Result: Forty-six studies were included. Meta-analysis showed that the outcome of the Fugl-Meyer Upper Extremity assessment (FM-UE) (SMD = 0.20, P = 0.001) and activity function post intervention was significantly higher (SMD = 0.32, P < 0.001) in the RAT group than in the control group. Differences in outcomes of the FM-UE and activity function between the RAT group and control group were observed at the end of treatment and were not found at the follow-up. Additionally, the outcomes of the FM-UE (SMD = 0.15, P = 0.005) and activity function (SMD = 0.32, P = 0.002) were significantly different between the RAT and control groups only with a total training time of more than 15 h. Moreover, the differences in outcomes of FM-UE and activity post intervention were not significant when the arm robots were applied to patients with severe impairments (FM-UE: SMD = 0.14, P = 0.08; activity: SMD = 0.21, P = 0.06) or when patients were provided with patient-passive training (FM-UE: SMD = - 0.09, P = 0.85; activity: SMD = 0.70, P = 0.16).

Conclusion: RAT has the significant immediate benefits for motor control and activity function of hemiparetic upper limb in patients after stroke compared with controls, but there is no evidence to support its long-term additional benefits. The superiority of RAT in improving motor control and activity function is limited by the amount of training time and the patients' active participation.
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http://dx.doi.org/10.1186/s12984-022-01058-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306153PMC
July 2022

Effects of dietary arachidonic acid (ARA) on immunity, growth and fatty acids of Apostichopus japonicus.

Fish Shellfish Immunol 2022 Jul 19;127:901-909. Epub 2022 Jul 19.

Department of Biotechnology, School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, Liaoning Province, PR China. Electronic address:

As an important aquaculture species, improving the immunity of cultured Apostichopus japonicus (A. japonicus) is vital for its health in aquaculture farming. It has been shown that ARA is an important metabolite for A. japonicus infected by Vibrio splendidus. In this study, we aimed to determine the effects of dietary exogenous ARA on healthy sea cucumber cultures, including assessments of immunity, growth, and fatty acid content. Five experimental diets containing 0.01%, 0.29%, 0.46%, 0.70%, and 1.09% ARA were tested. The specific growth rate (SGR) of sea cucumbers did not be significantly affected by exogenous ARA diet groups. The results showed that dietary ARA between 0.49 and 1.09% notably improved the survival rate of sea cucumbers infected by Vibrio splendidus compared with the control group without exogenous ARA. The results also showed the effects of dietary ARA on immune-related genes, enzymes, and oxidation indices; most of the exogenous ARA significantly upregulated the mRNA expression of the genes NFκB, TLR, TLR3, TRAF6, Toll, and MyD88. The activities of ACP, AKP, and lysozyme increased in the 0.49-1.09% ARA groups, especially the dietary 0.49% ARA group. The SOD1 and NOS activities were enhanced by dietary ARA between 0.29 and 0.70%. Compared with the control, the MDA content increased, but the 0.49% ARA-diet group had a lower MDA content. Based on these data, 0.49-0.70% ARA significantly enhanced immunity in cultured A. japonicus. Exogenous 0.49% and 0.70% ARA also elevated the ARA, total PUFA and n-6 PUFA in the body walls. In conclusion, the appropriate exogenous ARA (0.49%-0.70%) in diets could improve immunity and fatty acid content considerably. The results provide basic evidence that ARA can serve a useful immune enhancer for A. japonicus aquaculture.
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http://dx.doi.org/10.1016/j.fsi.2022.07.037DOI Listing
July 2022

Recurrent hypoglycemic coma and diabetic ketoacidosis caused by insulin antibody. A rare case of type 1 diabetes mellitus.

Rev Med Chil 2022 Jan;150(1):115-119

Department of Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Insulin antibodies (IAs) induced by exogenous insulin rarely cause hypoglycemia. However, insulin autoantibodies (IAAs) in insulin autoimmune syndrome (IAS) can cause hypoglycemia. The typical manifestations of IAS are fasting or postprandial hypoglycemia, elevated insulin level, decreased C-peptide levels, and positive IAA. We report a 45-year-old male with type 1 diabetes mellitus (T1DM) treated with insulin analogues suffering from recurrent hypoglycemic coma and diabetic ketoacidosis (DKA). His symptoms were caused by exogenous insulin and were similar to IAS. A possible reason was that exogenous insulin induced IA. IA titers were 61.95% (normal: < 5%), and the concentrations of insulin and C-peptide were > 300 mU/L and < 0.02 nmol/L when hypoglycemia occurred. Based on his clinical symptoms and other examinations, he was diagnosed with hyperinsulinemic hypoglycemia caused by IA. His symptoms improved after changing insulin regimens from insulin lispro plus insulin detemir to recombinant human insulin (Gensulin R) and starting prednisone.
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http://dx.doi.org/10.4067/S0034-98872022000100115DOI Listing
January 2022

Identification of Novel Organophosphate Esters in Hydroponic Lettuces ( L.): Biotransformation and Acropetal Translocation.

Environ Sci Technol 2022 Aug 18;56(15):10699-10709. Epub 2022 Jul 18.

MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.

The absorption, translocation, and biotransformation behaviors of organophosphate esters (OPEs) and diesters (OPdEs) in a hydroponic system were investigated. The lateral root was found as the main accumulation and biotransformation place of OPEs and OPdEs in lettuce. The nontarget analysis using high-resolution mass spectrometry revealed five hydroxylated metabolites and five conjugating metabolites in the OPE exposure group, among which methylation, acetylation, and palmitoyl conjugating OPEs were reported as metabolites for the first time. Particularly, methylation on phosphate can be a significant process for plant metabolism, and methyl diphenyl phosphate (MDPP) accounted for the majority of metabolites. The translocation factor values of most identified OPE metabolites are negatively associated with their predicted logarithmic octanol-water partitioning coefficient (log ) values (0.75-2.45), indicating that hydrophilicity is a dominant factor in the translocation of OPE metabolites in lettuce. In contrast, palmitoyl conjugation may lead to an enhanced acropetal translocation and those with log  values < 0 may have limited translocation potential. Additionally, OPE diesters produced from the biotransformation of OPEs in lettuce showed a higher acropetal translocation potential than those exposed directly. These results further emphasize the necessity to consider biotransformation as an utmost important factor in the accumulation and acropetal translocation potential of OPEs in plants.
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http://dx.doi.org/10.1021/acs.est.2c01610DOI Listing
August 2022

Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats.

Saudi J Biol Sci 2022 May 11;29(5):3354-3365. Epub 2022 Feb 11.

School of Medicine, College of Medicine and Health Science, Jigjiga University, Jigjiga, Ethiopia.

Background: Nano-based drug delivery systems have shown several advantages in cancer treatment like specific targeting of cancer cells, good pharmacokinetics, and lesser adverse effects. Liver cancer is a fifth most common cancer and third leading cause of cancer-related mortalities worldwide.

Objective: The present study focusses to formulate the selenium (S)/chitosan (C)/polyethylene glycol (Pg)/allyl isothiocyanate (AI) nanocomposites (SCPg-AI-NCs) and assess its therapeutic properties against the diethylnitrosamine (DEN)-induced liver cancer in rats via inhibition of oxidative stress and tumor markers.

Methodology: The SCPg-AI-NCs were synthesized by ionic gelation technique and characterized by various characterization techniques. The liver cancer was induced to the rats by injecting a DEN (200 mg/kg) on the 8th day of experiment. Then DEN-induced rats treated with 10 mg/kg of formulated SCPg-AI-NCs an hour before DEN administration for 16 weeks. The 8-hydroxy-2' -deoxyguanosine (8-OHdG) content, albumin, globulin, and total protein were examined by standard methods. The level of glutathione (GSH), vitamin-C & -E, and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were examined using assay kits. The liver marker enzymes i.e., alanine transaminase (ALT), aspartate tansaminase (AST), γ-glutamyl transaminase (GGT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activities, alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA), Bax, and Bcl-2 levels, and caspase-3&9 activities was examined using assay kits and the liver histopathology was assessed microscopically by hematoxylin and eosin staining method. The effect of formulated SCPg-AI-NCs on the viability and apoptotic cell death on the HepG2 cells were examined using MTT and dual staining assays, respectively.

Results: The results of different characterization studies demonstrated the formation of SCPg-AI-NCs with tetragonal shape, narrowed distribution, and size ranging from 390 to 450 nm. The formulated SCPg-AI-NCs treated liver cancer rats indicated the reduced levels of 8-OHdG, albumin, globulin, and total protein. The SCPg-AI-NCs treatment appreciably improved the GSH, vitamin-C & -E contents, and SOD, CAT, GPx, and GR activities in the serum of liver cancer rats. The SCPg-AI-NCs treatment remarkably reduced the liver marker enzyme activities in the DEN-induced rats. The SCPg-AI-NCs treatment decreased the AFP and CEA contents and enhanced the Bax and caspase 3&9 activities in the DEN-induced rats. The SCPg-AI-NCs effectively decreased the cell viability and induced apoptosis in the HepG2 cells.

Conclusion: The present findings suggested that the formulated SCPg-AI-NCs remarkably inhibited the DEN-induced liver carcinogenesis in rats. These findings provide an evidence that SCPg-AI-NCs can be a promising anticancer nano-drug in the future to treat the liver carcinogenesis.
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http://dx.doi.org/10.1016/j.sjbs.2022.02.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280227PMC
May 2022

Accurate classification of white blood cells by coupling pre-trained ResNet and DenseNet with SCAM mechanism.

BMC Bioinformatics 2022 Jul 15;23(1):282. Epub 2022 Jul 15.

Landing Artificial Intelligence Center for Pathological Diagnosis, Wuhan, 430072, China.

Background: Via counting the different kinds of white blood cells (WBCs), a good quantitative description of a person's health status is obtained, thus forming the critical aspects for the early treatment of several diseases. Thereby, correct classification of WBCs is crucial. Unfortunately, the manual microscopic evaluation is complicated, time-consuming, and subjective, so its statistical reliability becomes limited. Hence, the automatic and accurate identification of WBCs is of great benefit. However, the similarity between WBC samples and the imbalance and insufficiency of samples in the field of medical computer vision bring challenges to intelligent and accurate classification of WBCs. To tackle these challenges, this study proposes a deep learning framework by coupling the pre-trained ResNet and DenseNet with SCAM (spatial and channel attention module) for accurately classifying WBCs.

Results: In the proposed network, ResNet and DenseNet enables information reusage and new information exploration, respectively, which are both important and compatible for learning good representations. Meanwhile, the SCAM module sequentially infers attention maps from two separate dimensions of space and channel to emphasize important information or suppress unnecessary information, further enhancing the representation power of our model for WBCs to overcome the limitation of sample similarity. Moreover, the data augmentation and transfer learning techniques are used to handle the data of imbalance and insufficiency. In addition, the mixup approach is adopted for modeling the vicinity relation across training samples of different categories to increase the generalizability of the model. By comparing with five representative networks on our developed LDWBC dataset and the publicly available LISC, BCCD, and Raabin WBC datasets, our model achieves the best overall performance. We also implement the occlusion testing by the gradient-weighted class activation mapping (Grad-CAM) algorithm to improve the interpretability of our model.

Conclusion: The proposed method has great potential for application in intelligent and accurate classification of WBCs.
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http://dx.doi.org/10.1186/s12859-022-04824-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287918PMC
July 2022

TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.

Front Pharmacol 2022 27;13:938416. Epub 2022 Jun 27.

Department of Orthopaedics, The Second Hospital of Anhui Medical University, Hefei, China.

Following spinal cord injury (SCI), microglia gradually migrate to the edge of the lesion, interweaving around the border of the lesion to form the microglial scar, which performs inflammatory limiting and neuroprotective functions. Recent reports showed that Yes-associated protein (YAP) was expressed in astrocytes and promoted the formation of astrocytic scars, while YAP was not expressed in microglia after SCI. YAP and its paralogue transcriptional coactivator with PDZ-binding motif (TAZ) are transcriptional coactivators, which have a similar functional role as both are negatively regulated by the Hippo signalling pathway. However, the expression and function of TAZ after SCI are unclear. Our research group previously found that Fascin-1 was highly expressed in microglia and promoted migration of microglia after SCI, and that, there was a close regulatory relationship between Fascin-1 and YAP/TAZ. In this study, we demonstrated that TAZ was significantly upregulated and mainly expressed in microglia after SCI, and accumulated in the nuclei of microglia in the spinal cord at 14 days post-SCI. Moreover, TAZ was upregulated and accumulated in the nuclei of anti-inflammatory M2-like (M2-L) polarized or myelin-treated microglia. Additionally, XMU-MP-1 (an inhibitor of the Hippo kinase MST1/2 to active TAZ) promoted the aggregation of microglia around the lesion core, resulting in the formation of microglial scars and the functional recovery of mice after SCI. Our findings also indicated that TAZ promoted microglial migration . Mechanistically, Fascin-1 interacted with TAZ, which upregulated TAZ expression and induced TAZ nuclear accumulation in microglia to promote microglial migration. These findings revealed that TAZ mediated microglial migration to the edge of the lesion core, promoting the formation of microglial scars and functional recovery after SCI. Moreover, TAZ was downstream of Fascin-1, which positively regulated microglial migration after SCI.
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http://dx.doi.org/10.3389/fphar.2022.938416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271831PMC
June 2022

Locoregional anesthesia for pain control after microsurgical reconstruction of the lower extremities: Issues should be clarified.

J Plast Reconstr Aesthet Surg 2022 Jul 3. Epub 2022 Jul 3.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, NO. 95 Yong-An Road, Xi-Cheng District, Beijing 100050, P R China.

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http://dx.doi.org/10.1016/j.bjps.2022.06.095DOI Listing
July 2022

Screening of potent α-glucosidase inhibitory and antioxidant polyphenols in Prunella vulgaris L. by bioreaction-HPLC-quadrupole-time-of-flight-MS/MS and in silico analysis.

J Sep Sci 2022 Jul 12. Epub 2022 Jul 12.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, P. R. China.

Prunella vulgaris L. is a well-known traditional Chinese medicine for blood glucose homeostasis and antioxidant potential. Ethyl acetate fraction of P. vulgaris L. demonstrated higher phenolic content (85.53 ± 6.74 mg gallic acid equivalents per gram dry weight), α-glucosidase inhibitory (IC , 69.13 ± 2.86 μg/ml), and antioxidant (IC , 8.68 ± 1.01 μg/ml) activities. However, the bioactive polyphenols responsible for the beneficial properties remain unclear. Here, bioreaction-HPLC-quadrupole-time-of-flight-MS/MS method was developed for rapid, accurate, and efficient screening and identification of polyphenols with α-glucosidase inhibitory and antioxidant activities from P. vulgaris L. Bioactive polyphenols can specifically bind with α-glucosidase or react with 1,1-diphenyl-2-picryl-hydrazyl radical, which was easily discriminated from nonactive compounds. Subsequently, 20 bioactive polyphenols (16 phenyl propionic acid derivatives and four flavonoids) were screened and identified. Furthermore, molecular docking analysis revealed that screened 20 polyphenols bind with the active sites of α-glucosidase through hydrogen bonding and π-π stacking. Density functional theory calculations demonstrated their electron transport ability and chemical reactivity. The in silico analysis confirmed the screened results. In summary, this study provided a valuable strategy for rapid discovering bioactive compounds from complex natural products and offered scientific evidence for further development and application of P. vulgaris L.
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http://dx.doi.org/10.1002/jssc.202200374DOI Listing
July 2022
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