Publications by authors named "Chen Zhang"

2,388 Publications

  • Page 1 of 1

Using Extracochlear Multichannel Electrical Stimulation to Relieve Tinnitus and Reverse Tinnitus-Related Auditory-Somatosensory Plasticity in the Cochlear Nucleus.

Neuromodulation 2021 Aug 3. Epub 2021 Aug 3.

ENT Institute and Department of Otolaryngology & Head-Neck Surgery, Eye & ENT Hospital of Fudan University, Shanghai, 200031, China.

Objectives: Tinnitus has no reliable cure but may be significantly relieved by the usage of cochlear implants. However, not all tinnitus patients necessitate cochlear implantation that can impair hearing. This study was to investigate whether a novel extracochlear electrical stimulation (EES) strategy could relieve tinnitus of guinea pigs without hearing impairment, and the roles of auditory-somatosensory plasticity in the cochlear nucleus in the tinnitus relief.

Materials And Methods: We used a novel four-electrode extracochlear implant to electrically stimulate the cochlea of tinnitus guinea pigs. Tinnitus was assessed by the gap-prepulse inhibition of the acoustic startle reflex (GPIAS) ratios and the tinnitus index. The plasticity of auditory and somatosensory innervation in the different subdivisions of cochlear nucleus was evaluated by immunostaining of vesicular glutamate transporter 1 (VGLUT1) and VGLUT2, respectively.

Results: The EES induced significant decreases of GPIAS ratios and the tinnitus index of tinnitus guinea pigs, indicating reductions of tinnitus behavioral manifestations. Meanwhile, the EES reversed the abnormal auditory-somatosensory innervation in the cochlear nucleus of tinnitus animals but did not change the hearing and the numbers of inner hair cell synapses.

Conclusions: This study demonstrated that the novel EES strategy could effectively relieve tinnitus without impairment to hearing and cochlear structure of tinnitus animals. The reversal of tinnitus-related auditory-somatosensory plasticity in the cochlear nucleus was correlated with the tinnitus relief induced by the EES.
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http://dx.doi.org/10.1111/ner.13506DOI Listing
August 2021

A multi-country test of brief reappraisal interventions on emotions during the COVID-19 pandemic.

Nat Hum Behav 2021 Aug 2. Epub 2021 Aug 2.

Queen's University Belfast, Belfast, Ireland.

The COVID-19 pandemic has increased negative emotions and decreased positive emotions globally. Left unchecked, these emotional changes might have a wide array of adverse impacts. To reduce negative emotions and increase positive emotions, we tested the effectiveness of reappraisal, an emotion-regulation strategy that modifies how one thinks about a situation. Participants from 87 countries and regions (n = 21,644) were randomly assigned to one of two brief reappraisal interventions (reconstrual or repurposing) or one of two control conditions (active or passive). Results revealed that both reappraisal interventions (vesus both control conditions) consistently reduced negative emotions and increased positive emotions across different measures. Reconstrual and repurposing interventions had similar effects. Importantly, planned exploratory analyses indicated that reappraisal interventions did not reduce intentions to practice preventive health behaviours. The findings demonstrate the viability of creating scalable, low-cost interventions for use around the world. PROTOCOL REGISTRATION: The stage 1 protocol for this Registered Report was accepted in principle on 12 May 2020. The protocol, as accepted by the journal, can be found at https://doi.org/10.6084/m9.figshare.c.4878591.v1.
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http://dx.doi.org/10.1038/s41562-021-01173-xDOI Listing
August 2021

Design, synthesis and screening of a drug discovery library based on an Eremophila-derived serrulatane scaffold.

Phytochemistry 2021 Jul 30;190:112887. Epub 2021 Jul 30.

Griffith Institute for Drug Discovery, School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia. Electronic address:

Chemical studies of the aerial parts of the Australian desert plant Eremophila microtheca afforded the targeted and known diterpenoid scaffolds, 3,7,8-trihydroxyserrulat-14-en-19-oic acid and 3-acetoxy-7,8-dihydroxyserrulat-14-en-19-oic acid. The most abundant serrulatane scaffold was converted to the poly-methyl derivatives, 3-hydroxy-7,8-dimethoxyserrulat-14-en-19-oic acid methyl ester and 3,7,8-trimethoxyserrulat-14-en-19-oic acid methyl ester using simple and rapid methylation conditions consisting of DMSO, NaOH and MeI at room temperature. Subsequently a 12-membered amide library was synthesised by reacting the methylated scaffolds with a diverse series of commercial primary amines. The chemical structures of the 12 undescribed semi-synthetic analogues were fully characterised following 1D/2D NMR, MS, UV, ECD and [α] data analyses. All compounds were evaluated for their anthelmintic, anti-microbial and anti-viral activities. While none of the compounds significantly inhibited motility or development of the exsheathed third-stage larvae (xL3s) of a pathogenic ruminant parasite, Haemonchus contortus, the tri-methylated analogue induced a skinny phenotype in fourth-stage larvae (L4s) after seven days of treatment (IC = 14 μM). Anti-bacterial and anti-fungal activities were not observed at concentrations up to 20 μM. Activity against HIV latency reversal was tested in inducible, chronically-infected cells, with the tri-methylated analogue being the most active (EC = 38 μM).
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http://dx.doi.org/10.1016/j.phytochem.2021.112887DOI Listing
July 2021

Selective Catalysis Remedies Polysulfide Shuttling in Lithium-Sulfur Batteries.

Adv Mater 2021 Aug 2:e2101006. Epub 2021 Aug 2.

Nanoyang Group, State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

The shuttling of soluble lithium polysulfides between the electrodes leads to serious capacity fading and excess use of electrolyte, which severely bottlenecks practical use of Li-S batteries. Here, selective catalysis is proposed as a fundamental remedy for the consecutive solid-liquid-solid sulfur redox reactions. The proof-of-concept Indium (In)-based catalyst targetedly decelerates the solid-liquid conversion, dissolution of elemental sulfur to polysulfides, while accelerates the liquid-solid conversion, deposition of polysulfides into insoluble Li S, which basically reduces accumulation of polysulfides in electrolyte, finally inhibiting the shuttle effect. The selective catalysis is revealed, experimentally and theoretically, by changes of activation energies and kinetic currents, modified reaction pathway together with the probed dynamically changing catalyst (LiInS catalyst), and gradual deactivation of the In-based catalyst. The In-based battery works steadily over 1000 cycles at 4.0 C and yields an initial areal capacity up to 9.4 mAh cm with a sulfur loading of ≈9.0 mg cm .
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http://dx.doi.org/10.1002/adma.202101006DOI Listing
August 2021

Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal.

Stem Cells Int 2021 10;2021:5522723. Epub 2021 Jul 10.

Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, China.

Dax1(also known as Nr0b1) is regarded as an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax1 knockout (KO) cell line using the CRISPR/Cas9 system to analyze the precise function of Dax1. We reported that 2i/LIF-ESCs had significantly lower Dax1 expression than LIF/serum-ESCs. Dax1KO ES cell lines could be established in 2i/LIF and their pluripotency was confirmed. In contrast, Dax1-null ESCs could not be continuously passaged in LIF/serum due to severe differentiation and apoptosis. In LIF/serum, the activities of the Core module and Myc module were significantly reduced, while the PRC2 module was activated after Dax1KO. The expression of most proapoptotic genes and lineage-commitment genes were drastically increased, while the downregulated expression of antiapoptotic genes and many pluripotency genes was observed. Our research on the pluripotent state-dependent role of Dax1 provides clues to understand the molecular regulation mechanism at different stages of early embryonic development.
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http://dx.doi.org/10.1155/2021/5522723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286181PMC
July 2021

Global evolution of research on green energy and environmental technologies:A bibliometric study.

J Environ Manage 2021 Jul 28;297:113382. Epub 2021 Jul 28.

College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha, 410082, China.

To better grasp developments and trends in research on green energy and environmental technologies, the published literatures in the Web of Science Core Collection database from 1998 to 2020 were utilized to reveal critical information and guidance on what has been investigated, and what are the changes in research interests using a bibliometric method. Herein, yearly quantitative distribution of literatures, author contribution and collaboration, productive and influential institutions and countries/territories, co-citation analysis, keywords co-occurrence analysis, and research frontier identification are analyzed via information visualization technology. The results show that the publications on green energy and environmental technologies have grown exponentially, that China, the USA, and Italy are the most active countries, but the global cooperation is not close at present. The research frontier identification results reveal that the categories of energy, wastewater, and performance remain stable, while the trending up and emerging categories of catalyst and CO emission show clear shift over the last decade, indicating that catalytic production of clean energy and value-added chemicals, strategies to reduce greenhouse gas emissions, and other related studies to solve the global energy crisis and environmental problems are the research frontiers. This bibliometric study provides unique insights and offers research guidance on green energy and environmental technologies.
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http://dx.doi.org/10.1016/j.jenvman.2021.113382DOI Listing
July 2021

Construction of Metal Hydrate-Based Amorphous Magnetic Nanosheets for Enhanced Protein Enrichment and Immobilization.

ACS Appl Mater Interfaces 2021 Jul 30. Epub 2021 Jul 30.

Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Inspired by the hierarchical fabrication technique, many self-assembly procedures have improved the construction of nanomaterials with unique physicochemical characteristics and multiple functions. The generation of multiple complexes is always accompanied by hierarchical structures and intriguing properties that are distinct from their individual segments. An interesting composite is amorphous magnetic Zn-Zr phosphate hydrated nanosheets (Zn-Zr APHNs), generated using templated synthesis and nanoparticle codeposition. The special porous structure of this construct, together with the abundance of metal ions and hydrate present, endows it with many interaction sites for proteins, provides high loading efficiency, and enhances bioactivity. Then, a series of proteins, including enzymes, was immobilized by the Zn-Zr APHNs by multiple interactions, high ionization, and larger surface of the nanosheets. In this study, novel methods for the enrichment of bioactive proteins while retaining the activity of protein payloads are presented. As a verification method, it is indicated that the Zn-Zr APHNs can deliver enzyme proteins (i.e., Cyt-c) to increase the catalytic activity with their biological function and structural integrity, resulting in a highly increased activity to free proteins.
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http://dx.doi.org/10.1021/acsami.1c10086DOI Listing
July 2021

Present and future prospects for wheat improvement through genome editing and advanced technologies.

Plant Commun 2021 Jul 5;2(4):100211. Epub 2021 Jun 5.

Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

Wheat (, 2 = 6 = 42, AABBDD) is one of the most important staple food crops in the world. Despite the fact that wheat production has significantly increased over the past decades, future wheat production will face unprecedented challenges from global climate change, increasing world population, and water shortages in arid and semi-arid lands. Furthermore, excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration. To ensure global food and ecosystem security, it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies. However, the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement, thus impeding the breeding of elite wheat cultivars. In this review, we first introduce state-of-the-art genome-editing technologies in crop plants, especially wheat, for both functional genomics and genetic improvement. We then outline applications of other technologies, such as GWAS, high-throughput genotyping and phenotyping, speed breeding, and synthetic biology, in wheat. Finally, we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies. We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement of wheat for sustainable global production.
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http://dx.doi.org/10.1016/j.xplc.2021.100211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299080PMC
July 2021

Light-Controlled Precise Delivery of NIR-Responsive Semiconducting Polymer Nanoparticles with Promoted Vascular Permeability.

Adv Healthc Mater 2021 Jul 26:e2100569. Epub 2021 Jul 26.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

The endothelial barrier plays an essential role in health and disease by protecting organs from toxins while allowing nutrients to access the circulation. However, it is the major obstacle that limits the delivery of therapeutic drugs to the diseased tissue. Here, it is reported for the first time that near-infrared (NIR) laser pulses can transiently promote the delivery of semiconducting polymer nanoparticles passing the vascular barrier via photoacoustic-effect-induced accumulation, only by the aid of pulse laser irradiation. This strategy enables selective and substantial accumulation of the NIR-absorbing nanoparticles inside specific tissues, implying the discovery of an unprecedented approach for light-controlled nanoparticle delivery. Especially, the nanoparticle delivery in solid tumors by 10-min laser scanning is approximately six times higher than that of the enhanced permeability and retention (EPR) effect in 24 h under current experimental conditions. Further results confirm that this strategy facilitates substantial accumulation of nanoparticles in the mouse brain with intact skull. This approach thus opens a new door for tissue-specific delivery of nanomaterials with an unprecedented level of efficiency and precision.
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http://dx.doi.org/10.1002/adhm.202100569DOI Listing
July 2021

Efficacy and tolerability of pharmacological interventions on metabolic disturbance induced by atypical antipsychotics in adults: A systematic review and network meta-analysis.

J Psychopharmacol 2021 Jul 27:2698811211035391. Epub 2021 Jul 27.

Schizophrenia Program, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: There have been a few systematic reviews and conventional meta-analyses reporting effect of drugs on metabolic disturbance induced by atypical antipsychotics (AAPs). However, few of them provided sufficient and comprehensive comparisons between pharmacological interventions.

Aims: We aimed to qualitatively compare drugs' effect on AAPs-induced metabolic abnormalities by using network meta-analysis (NMA).

Methods: We searched PubMed, EMBASE, Web of Science, Cochrane Controlled Register of Trials (CENTRAL), and PsycINFO on March 26, 2019. Of 5889 records identified, 61 randomized clinical trials including 3467 participants were included. We estimated weighted mean difference (WMD) and odds ratio (OR) using NMA. We assessed the risk of bias of individual studies with the Review Manager 5.3. Primary outcomes included change of body weight and body mass index (BMI). Secondary outcomes included change of other cardiometabolic risk factors, acceptability, and tolerability.

Results: For body weight, topiramate (WMD -5.4, 95% CI -7.12 to -3.68), zonisamide (-3.44, 95% CI -6.57 to -0.36), metformin (-3.01, 95% CI -4.22 to -1.83), glucagon-like peptide-1 receptor agonists (GLP-1RAs) (-3.23, 95% CI -5.47 to -0.96), and nizatidine (-2.14, 95% CI -4.01 to -0.27) were significantly superior to placebo. Results regarding to BMI were similar to that of body weight. With respect to tolerability, only topiramate (OR 24, 95% CI 3.15 to 648) was inferior to placebo.

Conclusions: Considering both efficacy and tolerability, evidence from this NMA indicates zonisamide, metformin, GLP-1RAs, and nizatidine in adults should be the first-line treatment for alleviating AAPs-induced weight gain or elevated BMI.
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http://dx.doi.org/10.1177/02698811211035391DOI Listing
July 2021

Response of microorganisms to phosphate nanoparticles in Pb polluted sediment: Implications of Pb bioavailability, enzyme activities and bacterial community.

Chemosphere 2021 Jul 21;286(Pt 1):131643. Epub 2021 Jul 21.

College of Environmental Science and Engineering, Hunan University, Changsha, Hunan, 410082, China.

In recent years, various phosphate nanoparticles (PNPs) have been synthesized and applied for in situ Pb remediation in laboratory investigations. Here, three kinds of PNPs, CMC-nClAP (carboxymethyl cellulose stabilized nano-chlorapatite), SDS-nClAP (sodium dodecyl sulfate stabilized nano-chlorapatite) and Rha-nClAP (rhamnolipid stabilized nano-chlorapatite) were used to investigate the influence of PNPs on Pb bioavailability, enzyme activities and bacterial community in Pb polluted sediment. Pb bioavailability can be reduced by the application of CMC-nClAP, SDS-nClAP and Rha-nClAP with the maximum increases of residual fraction to 57.2 %, 58.3 % and 61.4 %, respectively. Alternatively, catalase activity, urease activity and protease activity also changed with the remediation of PNPs. Microbes responded quickly to PNPs in different ways: bacterial richness was all increased while bacterial diversity was only increased with the application of SDS-nClAP. Three dominant species, Proteobacteria, Firmicutes and Bacteroidetes were redistributed differentially during the treatment of PNPs. Interestingly, PNPs didn't significantly change the bacterial community structure in treated samples and CMC-nClAP induced fewer changes in microbial activity and community as compared with SDS-nClAP and Rha-nClAP. Overall, our findings suggested that long-term exposure to PNPs would decrease Pb bioavailability, regulate enzyme activities and affect bacterial community in sediments. The Pb bioavailability, physical-chemical properties of PNPs and properties of chemical/bio-surfactant may determine the response of microorganisms to PNPs in Pb polluted sediment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131643DOI Listing
July 2021

Rapid Separation and Efficient Removal of Cd Based on Enhancing Surface Precipitation by Carbonate-Modified Biochar.

ACS Omega 2021 Jul 8;6(28):18253-18259. Epub 2021 Jul 8.

Hunan Province Key Laboratory of Coal Resources Clean Utilization and Mine Environment Protection, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.

The high buoyancy of biochar makes its application difficult in Cd removal. In this paper, the content of minerals was increased by modifying carbonate on the biochar surface using the vacuum impregnation method. Enhancing surface precipitation between minerals and Cd introduced a correspondingly great number of Cd precipitates on the biochar surface, leading to the rapid precipitation and separation of buoyant biochar. The physical and chemical properties of carbonate-modified biochar and the adsorption mechanism of Cd were comprehensively studied by jar tests, scanning electron microscopy-energy-dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The results showed that the adsorption of Cd by carbonate-modified biochar was controlled by multiple mechanisms, including surface precipitation, surface complexation, and Cd-π interaction. Surface precipitation dominated the removal of Cd. The contributions of Cd removal mechanisms indicated that the contribution proportions of minerals increased from 89.73 to 97.9% when the pyrolysis temperature increased from 300 to 600 °C, while the contribution proportions of Cd-π binding decreased from 9.99 to 2.08%. Meanwhile, oxygen functional groups have only a marginal effect on Cd adsorption. Besides, the results revealed that the higher surface hydrophobicity and the lower polarity were conducive to biochar separation from water. The Cd removal method can provide efficient adsorption and rapid separation, making it possible to use biochar in water treatment.
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http://dx.doi.org/10.1021/acsomega.1c02126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296603PMC
July 2021

Identification of tumor microenvironment-related prognostic genes in colorectal cancer based on bioinformatic methods.

Sci Rep 2021 Jul 22;11(1):15040. Epub 2021 Jul 22.

Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun, Jilin, People's Republic of China.

Colorectal cancer (CRC) ranks fourth among the deadliest cancers globally, and the progression is highly affected by the tumor microenvironment (TME). This study explores the relationship between TME and colorectal cancer prognosis and identifies prognostic genes related to the CRC microenvironment. We collected the gene expression data from The Cancer Genome Atlas (TCGA) and calculated the scores of stromal/immune cells and their relations to clinical outcomes in colorectal cancer by the ESTIMATE algorithm. Lower immune scores were significantly related to the malignant progression of CRC (metastasis, p = 0.001). We screened 292 differentially expressed genes (DEGs) by dividing CRC cases into high and low stromal/immune score groups. Functional enrichment analyses and protein-protein interaction (PPI) networks illustrated that these DEGs were closely involved in immune response, cytokine-cytokine receptor interaction, and chemokine signaling pathway. Six DEGs (FABP4, MEOX2, MMP12, ERMN, TNFAIP6, and CHST11) with prognostic value were identified by survival analysis and validated in two independent cohorts (GSE17538 and GSE161158). The six DEGs were significantly related to immune cell infiltration levels based on the Tumor Immune Estimation Resource (TIMER). The results might contribute to discovering new diagnostic and prognostic biomarkers and new treatment targets for colorectal cancer.
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http://dx.doi.org/10.1038/s41598-021-94541-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298640PMC
July 2021

Comprehensive analysis of BTN3A1 in cancers: mining of omics data and validation in patient samples and cellular models.

FEBS Open Bio 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Butyrophilin 3A1 (BTN3A1), a major histocompatibility complex-associated gene that encodes a membrane protein with two extracellular immunoglobulin domains and an intracellular B30.2 domain, is critical in T-cell activation and adaptive immune response. Here, the expression of BTN3A1 in cancers was analyzed in eight databases comprising 86 733 patients of 33 cancers, and the findings were validated in patient samples and cell models. We showed that BTN3A1 was expressed in most cancers, and its expression level was strongly correlated with clinical outcome of 13 cancers. Mutations of BTN3A1 were detected, and the mutations were distributed throughout the entire gene. Gene set enrichment analysis showed that BTN3A1 co-expression genes and interacting proteins were enriched in immune regulation-related pathways. BTN3A1 was associated with tumor-infiltrating immune cells and was co-expressed with multiple immune checkpoints in patients with breast cancer (BRCA) and non-small cell lung cancer (NSCLC). We reported that BTN3A1 was downregulated in 46 of 65 (70.8%) NSCLCs, and its expression level was inversely associated with clinical outcome of the patients. BTN3A1 in tumor samples was lower than in counterpart normal tissues in 31 of 38 (81.6%) BRCAs. Bioinformatics analyses showed that BTN3A1 could be a target gene of transcription factor Spi-1 proto-oncogene (SPI1), and our 'wet' experiments showed that ectopic expression of SPI1 upregulated, whereas silencing of SPI1 downregulated, BTN3A1 expression in cells. These results suggest that BTN3A1 may function as a tumor suppressor and may serve as a potential prognostic biomarker in NSCLCs and BRCAs.
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http://dx.doi.org/10.1002/2211-5463.13256DOI Listing
July 2021

Color-Tunable All-Fluorescent White Organic Light-Emitting Diodes with a High External Quantum Efficiency Over 30% and Extended Device Lifetime.

Adv Mater 2021 Jul 22:e2103102. Epub 2021 Jul 22.

Key Lab of Organic Optoelectronics and Molecular Engineering of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

White organic light-emitting diodes (WOLEDs) with high efficiencies and tunable colors attracts considerable interest from the industry and academia. Thermally activated delayed-fluorescence (TADF) emitters can revolutionize such WOLED devices; however, they still suffer from poor performances. In this study, an advanced double-emissive-layer device architecture capable of hole-trapping TADF-sensitized emissions is proposed to not only achieve a recombination zone shift for the tunable colors but also accelerate exciton emission dynamics for high efficiency and alleviated roll-off. The proof-of-concept WOLEDs exhibit significant shifts in their Commission Internationale de l'Eclairage (CIE) coordinates and correlated color temperatures from (0.40, 0.47) and 4088 K at 100 cd m to (0.27, 0.33) and 9269 K at 5000 cd m . Additionally, the maximum external quantum efficiency (EQE) reaches 30.7% and remains >25% over a wide luminance range of 500-5000 cd m , along with an extended LT80 of over 20 000 h at an initial luminance of 100 cd m . This is the first time that all-fluorescent WOLEDs have been used to realize an EQE exceeding 30%, thereby establishing a new benchmark in this field.
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http://dx.doi.org/10.1002/adma.202103102DOI Listing
July 2021

Targeted extracellular vesicle delivery systems employing superparamagnetic iron oxide nanoparticles.

Acta Biomater 2021 Jul 18. Epub 2021 Jul 18.

Department of Gastroenterology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. Electronic address:

In the past decade, the study of extracellular vesicles (EVs), especially exosomes (50-150 nm) have attracted growing interest in numerous areas of cancer and tissue regeneration due to their unique biological features. A low isolation yield and insufficient targeting abilities limit their therapeutic applicability. Recently, superparamagnetic iron oxide nanoparticles (SPIONs) with magnetic navigation have been exploited to enhance the targeting ability of EVs. To construct targeted EV delivery systems engineered by SPIONs, several groups have pioneered the use of different techniques, such as electroporation, natural incubation, and cell extrusion, to directly internalize SPIONs into EVs. Furthermore, some endogenous ligands, such as transferrins, antibodies, aptamers, and streptavidin, were shown to enable modification of SPIONs, which increases binding with EVs. In this review, we summarized recent advances in targeted EV delivery systems engineered by SPIONs and focused on the key methodological approaches and the current applications of magnetic EVs. This report aims to address the existing challenges and provide comprehensive insights into targeted EV delivery systems. STATEMENT OF SIGNIFICANCE: Targeted extracellular vesicle (EV) delivery systems engineered by superparamagnetic iron oxide nanoparticles (SPIONs) have attracted wide attention and research interest in recent years. Such strategies employ external magnet fields to manipulate SPION-functionalized EVs remotely, aiming to enhance their accumulation and penetration in vivo. Although iron oxide nanoparticle laden EVs are interesting, they are controversial at present, hampering the progress in their clinical application. A thorough integration of these studies is needed for an advanced insight and rational design of targeted EV delivery systems. In this review, we summarize the latest advances in the design strategies of targeted EV delivery systems engineered by SPIONs with a focus on their key methodological approaches, current applications, limitation and future perspectives, which may facilitate the development of natural theranostic nanoplatforms.
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http://dx.doi.org/10.1016/j.actbio.2021.07.027DOI Listing
July 2021

Immunologic mechanism of fungal keratitis.

Int J Ophthalmol 2021 18;14(7):1100-1106. Epub 2021 Jul 18.

Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin 300384, China.

Fungal keratitis (FK) is a refractory disease that poses a serious threat to vision, with common risk factors like eye trauma, contact lens wearing, topical corticosteroids and antibiotic abuse. Nowadays, topical and systemic anti-fungal drugs and ocular surgeries are still the main therapeutic modalities. However, the pathogenesis of FK, especially the immunologic mechanism within it, has not yet been deeply clarified. A better understanding of the pathogenesis of FK is imperative for more effective therapies and prognosis. Meanwhile, the immune protection strategies are also urgently required to manage FK. This review highlights recent advances in the immunologic mechanism in the pathogenesis of FK, in hope of providing valuable reference information for more effective anti-fungal treatment.
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http://dx.doi.org/10.18240/ijo.2021.07.20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243179PMC
July 2021

Multifaceted role of phyto-derived polyphenols in nanodrug delivery systems.

Adv Drug Deliv Rev 2021 Jul 16:113870. Epub 2021 Jul 16.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address:

As naturally occurring bioactive products, several lines of evidence have shown the potential of polyphenols in the medical intervention of various diseases, including tumors, inflammatory diseases, and cardiovascular diseases. Notably, owing to the particular molecular structure, polyphenols can combine with proteins, metal ions, polymers, and nucleic acids providing better strategies for polyphenol-delivery strategies. This contributes to the inherent advantages of polyphenols as important functional components for other drug delivery strategies, e.g., protecting nanodrugs from oxidation as a protective layer, improving the physicochemical properties of carbohydrate polymer carriers, or being used to synthesize innovative functional delivery vehicles. Polyphenols have emerged as a multifaceted player in novel drug delivery systems, both as therapeutic agents delivered to intervene in disease progression and as essential components of drug carriers. Although an increasing number of studies have focused on polyphenol-based nanodrug delivery including epigallocatechin-3-gallate, curcumin, resveratrol, tannic acid, and polyphenol-related innovative preparations, these molecules are not without inherent shortcomings. The active biochemical characteristics of polyphenols constitute a prerequisite to their high-frequency use in drug delivery systems and likewise to provoke new challenges for the design and development of novel polyphenol drug delivery systems of improved efficacies. In this review, we focus on both the targeted delivery of polyphenols and the application of polyphenols as components of drug delivery carriers, and comprehensively elaborate on the application of polyphenols in new types of drug delivery systems. According to the different roles played by polyphenols in innovative drug delivery strategies, potential limitations and risks are discussed in detail including the influences on the physical and chemical properties of nanodrug delivery systems, and their influence on normal physiological functions inside the organism.
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http://dx.doi.org/10.1016/j.addr.2021.113870DOI Listing
July 2021

Oral Core-Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy.

ACS Appl Mater Interfaces 2021 Jul 15;13(29):33948-33961. Epub 2021 Jul 15.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China.

Although magnolol (Mag), an anti-inflammatory natural compound, has been demonstrated to play protective effects on ulcerative colitis (UC), its application as an alternative therapeutic reagent for UC treatment is still greatly impeded due to its poor stability in the gastrointestinal tract and insufficient accumulation in the inflamed colon lesion. Nano-/microsized drug delivery systems can potentially overcome some challenges regarding the oral administration of phytochemicals, which still confront premature early drug release, degradation of NPs, or the sustained drug release of MPs. In this study, we primarily loaded Mag into the core-shell zein-based nanoparticles with chondroitin sulfate coating ([email protected] NPs) with an average size of 142.27 ± 5.11 nm, showing significant macrophage-targeting and enhanced colon epithelial cellular uptake capacity. Then, we embedded [email protected] NPs into hydrogel microspheres via an electrospraying technology. The [email protected] NPsinMPs presented a uniform-sized sphere with an average size of 164.36 ± 6.29 μm and sustained drug-release profiles. Compared to CS-Zein NPs, the developed CS-Zein NPsinMPs exhibited prolonged colon retention on the inflammatory surface, as seen from and imaging fluorescence adhesion experiments. Based on the advantage of the combination of hybrid nanoparticles-in-microparticles, oral administration of [email protected] NPsinMPs significantly alleviated colitis symptoms in DSS-treated mice by regulating the expression levels of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) and anti-inflammatory cytokines (IL-10) and factor accelerated colonic mucosal barrier repair via upregulating the expression of ZO-1 and occludin. This study provides great insights into the oral drug delivery of natural compounds for UC therapy.
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http://dx.doi.org/10.1021/acsami.1c09804DOI Listing
July 2021

MiR-373 Inhibits the Epithelial-Mesenchymal Transition of Prostatic Cancer via Targeting Runt-Related Transcription Factor 2.

J Healthc Eng 2021 29;2021:6974225. Epub 2021 Jun 29.

Department of Urology Surgery, The Affiliated Tengzhou Central People's Hospital of Jining Medical University, Tengzhou, Shandong 277500, China.

Prostatic cancer (PCa) is a prevalent form of malignancy based on its high associated levels of mortality and morbidity across the world. MicroRNAs (miRNAs) are significant in the advancement of prostatic cancer. The current study is aimed at exploring the potential roles of miR-373 in PCa. In turn, the study conducted a qRT-PCR test to determine the levels of mRNA. A western blot test was also executed in determining the protein level. The processes of transwell assay and wound healing were integrated in the detection of the potential for PCa cells to invade and migrate. The integration of dual luciferase reporter assay is critical in determining the levels of luciferase activity among prostatic cancer cells. Then, the results showed a net decrease of miR-373 within prostatic cancer cells and tissues. Upregulated miR-373 reduced the invasion and migration potential of PCa cells. Moreover, overexpressed miR-373 increased the levels of E-cadherin and FSP1 as epithelial cell markers. Similarly, the overregulation of miR-373 brought about the upregulation of mesenchymal markers (N-cadherin, Snail, and vimentin). The study predicted runt-related transcription factor 2 (RUNX2) to be a target of miR-373. The luciferase activity of PCa cells was decreased after the cotransfection with miR-373 mimics and RUNX2 3' untranslated region (3'UTR) wild type (WT). Moreover, RUNX2 became upregulated in PCa cells and tissues. The upregulation of miR-373 decreased the mRNA and protein level of RUNX2. However, overexpressed RUNX2 abated the roles of miR-373 in the intrusion and migration of PCa cells and in regulating the expression of epithelial cell markers and mesenchymal markers. In short, miR-373 may regulate the EMT of PCa cells via targeting RUNX2. The miR-373/RUNX2 axis provides a therapeutic target for PCa.
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http://dx.doi.org/10.1155/2021/6974225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260310PMC
June 2021

SC-Cut Quartz Resonators for Dynamic Liquid Viscosity Measurements.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Jul 13;PP. Epub 2021 Jul 13.

This paper proposes an innovative viscosity sensor based on the thickness-shear vibration of an SC-cut quartz resonator. The thickness-shear mode is firstly analyzed and further studied with fluid-structure interaction between the resonator and the viscous fluid loading. The characteristic equation is derived based on the 3D linear piezoelectric equations and solved for sensitivity analysis. Then laboratory experiment is carried out to validate the theory. To conduct the viscosity measurement, the SC-cut quartz resonator is integrated with a U-tube test fixture, which is designed and fabricated for sensor housing to avoid the influence of the mass of the fluid. The resonator is tested with various viscosities by tuning the ratio of glycerol/water mixture. Experiment results show consistency with the analytical solution, which together present an improved sensitivity of viscosity measurement by using SC-cut quartz resonator comparing to other resonator-based viscosity sensors. The proposed viscosity sensor is sensitive, accurate, and portable, and therefore can be applied to real-time, on-site measurement or sampling of fluidic samples.
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http://dx.doi.org/10.1109/TUFFC.2021.3096782DOI Listing
July 2021

Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker.

Research (Wash D C) 2021 19;2021:9798580. Epub 2021 Jun 19.

Center for Molecular Imaging Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Stem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1,000-1,700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100 m single-cell cluster spatial resolution, along with the lung contraction and heart beating. As such, this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.
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http://dx.doi.org/10.34133/2021/9798580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237598PMC
June 2021

The role of oxidative stress in the susceptibility of noise-impaired cochleae to synaptic loss induced by intracochlear electrical stimulation.

Neuropharmacology 2021 Jul 8;196:108707. Epub 2021 Jul 8.

Department of Otolaryngology - Head and Neck Surgery, Eye & ENT Hospital of Fudan University, Shanghai, 200031, China; NHC Key Laboratory of Hearing Medicine, Shanghai, China. Electronic address:

Intracochlear electrical stimulation (ES) generated by cochlear implants (CIs) is used to activate auditory nerves to restore hearing perception in deaf subjects and those with residual hearing who use electroacoustic stimulation (EAS) technology. Approximately 1/3 of EAS recipients experience loss of residual hearing a few months after ES activation, but the underlying mechanism is unknown. Clinical evidence indicates that the loss is related to the previous history of noise-induced hearing loss (NIHL). In this report, we investigated the impact of intracochlear ES on oxidative stress levels and synaptic counts in inner hair cells (IHCs) of the apical, middle and basal regions of guinea pigs with normal hearing (NH) and NIHL. Our results demonstrated that intracochlear ES with an intensity of 6 dB above the thresholds of electrically evoked compound action potentials (ECAPs) could induce the elevation of oxidative stress levels, resulting in a loss of IHC synapses near the electrodes in the basal and middle regions of the NH cochleae. Furthermore, the apical region of cochleae with NIHL were more susceptible to synaptic loss induced by relatively low-intensity ES than that of NH cochleae, resulting from the additional elevation of oxidative stress levels and the reduced antioxidant capability throughout the whole cochlea.
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http://dx.doi.org/10.1016/j.neuropharm.2021.108707DOI Listing
July 2021

A New Residual Dense Network for Dance Action Recognition From Heterogeneous View Perception.

Front Neurorobot 2021 22;15:698779. Epub 2021 Jun 22.

College of Sports Art, Harbin Sport University, Harbin, China.

At present, part of people's body is in the state of sub-health, and more people pay attention to physical exercise. Dance is a relatively simple and popular activity, it has been widely concerned. The traditional action recognition method is easily affected by the action speed, illumination, occlusion and complex background, which leads to the poor robustness of the recognition results. In order to solve the above problems, an improved residual dense neural network method is used to study the automatic recognition of dance action images. Firstly, based on the residual model, the features of dance action are extracted by using the convolution layer and pooling layer. Then, the exponential linear element (ELU) activation function, batch normalization (BN) and Dropout technology are used to improve and optimize the model to mitigate the gradient disappearance, prevent over-fitting, accelerate convergence and enhance the model generalization ability. Finally, the dense connection network (DenseNet) is introduced to make the extracted dance action features more rich and effective. Comparison experiments are carried out on two public databases and one self-built database. The results show that the recognition rate of the proposed method on three databases are 99.98, 97.95, and 0.97.96%, respectively. It can be seen that this new method can effectively improve the performance of dance action recognition.
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http://dx.doi.org/10.3389/fnbot.2021.698779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258381PMC
June 2021

Development and validation of a new scoring system for the early diagnosis of tuberculous meningitis in adults.

Diagn Microbiol Infect Dis 2021 Apr 17;101(2):115393. Epub 2021 Apr 17.

Department of Neurology, the Third Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address:

We developed and validated a new diagnostic scoring system by simultaneously comparing 28 factors (including clinical, laboratory, and imaging) of HIV uninfected adult tuberculous meningitis (TBM) with viral meningitis (VM), bacterial meningitis (BM), and cryptococcal meningitis (CM). Predictors of TBM diagnosis obtained by logistic regression. A total of 382 patients with intracranial infection participated, and eight factors were independently associated with TBM diagnosis: symptom duration, evidence of extracranial tuberculosis, cerebrospinal fluid (CSF) leukocyte, CSF neutrophil, CSF protein, low serum sodium, meningeal enhancement, and tuberculomas. Factors are assigned according to weight, a score of ≥ 5 was suggestive of TBM with a sensitivity of 85.8% and a specificity of 87.7%, and the area under the receiver operating characteristic curve was 0.923. When applied to a prospective validation cohort, this scoring model showed robust performance. Our study suggests that the application of this score can help diagnose TBM more efficiently.
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http://dx.doi.org/10.1016/j.diagmicrobio.2021.115393DOI Listing
April 2021

NLRP3-activated bone marrow dendritic cells play antileukemic roles via IL-1β/Th1/IFN-γ in acute myeloid leukemia.

Cancer Lett 2021 Jul 5;520:109-120. Epub 2021 Jul 5.

Department of Hematology, Qilu Hospital, Shandong University, Jinan, 250012, China. Electronic address:

The bone marrow microenvironment of acute myeloid leukemia (AML) characterized by immunosuppressive features fosters leukemia immune escape. Elucidating the immunosuppressive mechanism and developing effective immunotherapeutic strategies are necessary. Here, we found that the Th1% and IFN-γ level were downregulated in bone marrow of AML and NLRP3-activated BMDCs promoted CD4 T cell differentiation into Th1 cells via IL-1β secretion. However, IFN-γ-producing Th1 cells were not induced by NLRP3-activated BMDCs in the presence of the NLRP3 inflammasome inhibitor MCC950 or anti-IL-1β antibody in vitro unless exogenous IL-1β was replenished. This inhibitory effect on Th1 differentiation was also observed in Nlrp3 mice or anti-IL-1β antibody-treated mice. Notably, elevated Th1 cell levels promoted apoptosis and inhibited proliferation in leukemia cells via IFN-γ secretion in vitro and in vivo. Thus, NLRP3-activated BMDCs promote the proliferation of IFN-γ-producing Th1 cells with antileukemic effects and may provide insight into the basis for leukemia immunotherapy in patients with AML.
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http://dx.doi.org/10.1016/j.canlet.2021.06.014DOI Listing
July 2021

Investigation of Adsorption Kinetics and the Isotherm Mechanism of Manganese by Modified Diatomite.

ACS Omega 2021 Jun 17;6(25):16402-16409. Epub 2021 Jun 17.

School of Resources and Safety Engineering, Central South University, Changsha 410083, China.

Natural diatomite modified by acetic acid (C-D) and sodium hydroxide (Na-D) for adsorbing manganese (Mn) was studied. The construction and morphology of the modified diatomite were then characterized by different efficient and accurate detection methods (Fourier transform infrared, scanning electron microscopy, and Brunauer-Emmett-Teller), and it was proved that C-D and Na-D increased the surface area and void volume compared to natural diatomite, and the modification of diatomite with acetic acid and alkali also significantly changed the structure of the functional groups of diatomite, especially in Na-D. Therefore, the adsorption rate of Mn by C-D and Na-D was higher than that of natural diatomite. The optimum conditions of Mn adsorption for C-D and Na-D were pH 5.0, 40 °C, 30 min and pH 5.0, 40 °C, 120 min, respectively, and this was best illustrated by pseudo-second-order kinetics. The Mn adsorption isotherm models showed that Mn adsorption on C-D and Na-D was stable, and the Langmuir adsorption isotherm model fitted adsorption processes of natural diatomite, C-D, and Na-D well as their correlation coefficients were 0.931, 0.940, and 0.991, respectively. These results suggested that modified diatomite with acetic acid and sodium hydroxide significantly increased the adsorption rate of Mn, which had an important application prospect for the remediation of Mn pollution in soil and water.
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http://dx.doi.org/10.1021/acsomega.1c01204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246448PMC
June 2021

Development of a Novel Restrictive Medium for Enrichment From Hongqu Based on the Synergistic Stress of Lactic Acid and Ethanol.

Front Microbiol 2021 21;12:702951. Epub 2021 Jun 21.

College of Chemical Engineering, Fuzhou University, Fuzhou, China.

Hongqu is a famous fermented food produced by and has been used as food coloring, wine starters and food additives for thousands of years in China. Excellent strain is an important prerequisite for producing high-quality Hongqu. However, the isolation of pure culture from Hongqu samples is time-consuming and laborious because it is easily interfered by other microorganisms (especially filamentous fungi). Therefore, the development of restrictive medium for enrichment from Hongqu is of great significance for the preparation and screening of excellent strains. Results of this study showed that has good tolerance to lactic acid and ethanol. Under the conditions of tolerance limits [7.5% lactic acid (v/v) and 12.0% ethanol (v/v)], could not grow but it still retained the vitality of spore germination, and the spore activity gradually decreased with the increasing concentrations of lactic acid and ethanol. More interestingly, the addition of lactic acid and ethanol significantly changed the microbial community structure in rice milk inoculated with Hongqu. After response surface optimization, could be successfully enriched without the interference of other microorganisms when 3.98% (v/v) lactic acid and 6.24% (v/v) ethanol were added to rice milk simultaneously. The optimal enrichment duration of by the restrictive medium based on the synergistic stress of lactic acid and ethanol is 8∼24 h. The synergistic stress of lactic acid and ethanol had no obvious effects on the accumulation of major metabolites in the progeny of , and was suitable for the enrichment of from different types of Hongqu. Finally, the possible mechanisms on the tolerance of to the synergistic stress of lactic acid and ethanol were preliminarily studied. Under the synergistic stress of lactic acid and ethanol, the cell membrane of defends against lactic acid and ethanol into cells to some extent, and the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities of were higher than those of other fungi, which significantly reduced the degree of lipid peroxidation of cell membrane, while secreting more amylase to make reducing sugars to provide the cells with enough energy to resist environmental stress. This work has great application value for the construction of strain library and the better development of its germplasm resources.
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http://dx.doi.org/10.3389/fmicb.2021.702951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256164PMC
June 2021

Application of the RCOG Risk Assessment Model for Evaluating Postpartum Venous Thromboembolism in Chinese Women: A Case-Control Study.

Med Sci Monit 2021 Jul 7;27:e929904. Epub 2021 Jul 7.

Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).

BACKGROUND Since China has not yet constructed its own risk assessment model (RAM) for pregnancy-related venous thromboembolism (VTE), more and more hospitals use the RCOG RAM for VTE risk prediction. However, the RCOG RAM was established based on Western populations, and its applicability in China is still uncertain. Thus, we aimed to evaluate the validity of the RCOG RAM in predicting postpartum VTE in Chinese maternity. MATERIAL AND METHODS This retrospective case-control study was conducted at the International Peace Maternity and Child Health Hospital (IPMCHH) from June 2016 to June 2020. The VTE group consisted of 38 women with postpartum VTE. For each VTE patient, 4 women without VTE who gave birth on the same day were randomly selected as the control group (n=152). The receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) were used to evaluate the discrimination, accuracy, and validity of the RCOG RAM. Univariable analysis and multivariable logistic regression analysis were used to identify other related factors for postpartum VTE. RESULTS Compared with the low-risk group, the risk of VTE was 9.75-fold greater in the intermediate-risk group, and 90.00-fold greater in the high-risk group. The area under curve (AUC) of the model was 0.828 (95% CI: 0.762-0.894), with a score of 2 as its best cut-off value, which exactly matched the criterion recommended by the RCOG guidelines for pharmacological thromboprophylaxis. The calibration curves and DCA of the model also showed good accuracy. In addition to the factors included in the RCOG RAM, glucocorticoid therapy during pregnancy (adjusted OR=6.72, 95% CI: 1.56-28.91) and previous use of IUD (adjusted OR=7.11, 95% CI: 1.45-34.93) were associated with increased risk of postpartum VTE. CONCLUSIONS The RCOG RAM was found to be effective in predicting postpartum VTE, and has certain guiding significance for postpartum thromboprophylaxis in China.
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http://dx.doi.org/10.12659/MSM.929904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274362PMC
July 2021

Rethinking olanzapine therapy for schizophrenia.

Asian J Psychiatr 2021 Aug 26;62:102741. Epub 2021 Jun 26.

Schizophrenia Program, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.ajp.2021.102741DOI Listing
August 2021
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