Publications by authors named "Chen Yuting"

190 Publications

Association of interleukin-6 promoter polymorphism with rheumatoid arthritis: a meta-analysis with trial sequential analysis.

Clin Rheumatol 2021 Sep 8. Epub 2021 Sep 8.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.

Objectives: The association of interleukin-6 (IL-6) -174G/C (rs1800795) and IL-6 -572G/C (rs1800796) single-nucleotide polymorphism (SNP) with the risk of acquiring rheumatoid arthritis (RA) was inconsistent among previous studies. This paper aims to investigate the association between IL-6 promoter polymorphism with RA in different ethnics.

Methods: Relevant studies were searched using Medline and Google Search engines; STATA software was used to perform the meta-analysis. Pooled odds ratios (OR) were calculated to estimate the potential genetic associations. Subgroup analysis and sensitivity analysis were applied to explore the sources of heterogeneity. Lastly, we used TSA (trial sequential analysis) software to verify the reliability of meta-analysis results.

Results: A total of 18 studies were included, involving 8116 subjects (3820 RA patients and 4296 controls). We found a tendency to associate RA with the IL-6 -174G/C allele in Asians (C vs G: OR = 4.56, 95% CI = 1.85-11.23; P < 0.001); with IL-6 -572G/C genotype or allele frequencies, there was no statistical differences between RA patients and controls (P > 0.05). TSA results indicate that the current meta-analysis can draw conclusions.

Conclusions: IL-6-174G/C gene polymorphism were associated with increased risk of RA in Asians, but not in Caucasians. There was no association between IL-6 -572G/C gene polymorphism and the risk of RA. Key Points • Although the association between interleukin-6 (IL-6) promoter polymorphism and rheumatic arthritis (RA) has been discussed in the previous meta-analysis, their conclusions are inconsistent. • In this study, trial sequential analysis (TSA) was introduced into the meta-analysis, and the following two important conclusions were confirmed: (1) IL-6-174G/C gene polymorphism was associated with increased risk of RA in Asians, but not in Caucasians. (2) There was no association between IL-6 -572G/C gene polymorphism and the risk of RA.
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http://dx.doi.org/10.1007/s10067-021-05886-2DOI Listing
September 2021

Effect of Exercise on Fatigue in Multiple Sclerosis Patients: A Network Meta-analysis.

Int J Sports Med 2021 Aug 10. Epub 2021 Aug 10.

Department of Epidemiology and Biostatistics, Anhui Medical University, Hefei, Anhui, China.

Few studies have directly compared the effects of different exercise therapies on reducing fatigue in patients with multiple sclerosis. Thus, we conducted a Frequentist network meta-analysis to analyze and compare the effectiveness of different types of exercise on reducing multiple sclerosis-related fatigue. Relevant randomized controlled trials were searched in PubMed, Web of Science and Cochrane Library databases from the date of their inception up to April 1, 2021. In total, 27 articles involving 1470 participants and 10 types of interventions met the inclusion criteria. The results indicated that aquatic exercise ranked as the most effective among these interventions, and aerobic exercise had small-to-moderate effect sizes. Most of the interventions were shown to be better than the control group, except for climbing. Climbing was the only intervention that ranked worse than the controls. All of these findings merit further investigation in future clinical trials.
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http://dx.doi.org/10.1055/a-1524-1935DOI Listing
August 2021

Prevalence of unmasked and improperly masked behavior in indoor public areas during the COVID-19 pandemic: Analysis of a stratified random sample from Louisville, Kentucky.

PLoS One 2021 28;16(7):e0248324. Epub 2021 Jul 28.

Department of Epidemiology and Population Health, School of Public Health and Information Sciences, University of Louisville, Louisville, Kentucky, United States of America.

Wearing a facial mask can limit COVID-19 transmission. Measurements of communities' mask use behavior have mostly relied on self-report. This study's objective was to devise a method to measure the prevalence of improper mask use and no mask use in indoor public areas without relying on self-report. A stratified random sample of retail trade stores (public areas) in Louisville, Kentucky, USA, was selected and targeted for observation by trained surveyors during December 14-20, 2020. The stratification allowed for investigating mask use behavior by city district, retail trade group, and public area size. The total number of visited public areas was 382 where mask use behavior of 2,080 visitors and 1,510 staff were observed. The average prevalence of mask use among observed visitors was 96%, while the average prevalence of proper use was 86%. In 48% of the public areas, at least one improperly masked visitor was observed and in 17% at least one unmasked visitor was observed. The average prevalence of proper mask use among staff was 87%, similar to the average among visitors. However, the percentage of public areas where at least one improperly masked staff was observed was 33. Significant disparities in mask use and its proper use were observed among both visitors and staff by public area size, retail trade type, and geographical area. Observing unmasked and improperly masked visitors was more common in small (less than 1500 square feet) public areas than larger ones, specifically in food and grocery stores as compared to other retail stores. Also, the majority of the observed unmasked persons were male and middle-aged.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248324PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318281PMC
August 2021

Rational Design of Self-Supported CuO -Decorated Composite Films as an Efficient and Easy-Recycling Catalyst for Styrene Oxidation.

ACS Omega 2021 Jul 6;6(28):18157-18168. Epub 2021 Jul 6.

School of Chemistry, Tiangong University, 399 Binshui West Road, Tianjin 300387, China.

The applications of graphene-based materials in catalysis are limited by their strong tendency to aggregate, which may lead to a decrease in active sites. Herein, we propose a facile and controllable strategy to fabricate a series of heterogeneous catalysts with a unique nanostructure wherein CuO -decorated reduced graphene oxide (rGO) sheets are incorporated into a solid matrix composed of poly(vinylpyrrolidone) (PVP) and carboxymethyl cellulose (CMC). The resultant materials are self-supported films and could be directly used as catalysts for the liquid-phase oxidation of styrene without the requirement for extra substrates. The employment of PVP-CMC (PC) as the support for CuO -decorated rGO sheets successfully inhibits their aggregation. Benefiting from the dispersion of copper species, these films exhibit good catalytic activity and recyclability under mild reaction conditions. Especially, they can be conveniently removed from the reaction mixture by tweezers due to their structural stability. For catalyzing multiple reactions with high efficiency and facile recyclability, this study offers a universal strategy to design heterogeneous catalysts based on graphene materials and provides a promising platform.
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http://dx.doi.org/10.1021/acsomega.1c02031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296588PMC
July 2021

A Pan-Cancer Analysis of CD161, a Potential New Immune Checkpoint.

Front Immunol 2021 9;12:688215. Epub 2021 Jul 9.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: CD161, encoded by killer cell lectin-like receptor B1 gene, is a newly reported candidate inhibitor of tumour-infiltrating T cells. Antibody-mediated CD161 blockade enhances T cell-mediated killing of cancer cells and in several tumour types. We evaluated the role of CD161 using The Cancer Genome Atlas (TCGA) Pan-Cancer Data.

Methods: CD161 expression was analysed using RNAseq data from TCGA and the Genotype-Tissue Expression (GTEx) database. HPA, GeneCards, and String database were used to explore the protein information of CD161. The prognostic value of CD161 was analysed using clinical survival data from the TCGA. Enrichment analysis of CD161 was conducted using the R package "clusterProfiler". We downloaded the immune cell infiltration score of TCGA samples from published articles and online databases and performed a correlation analysis between immune cell infiltration levels and CD161 expression. We further assessed the association between CD161 and immune checkpoints, immune activating genes, immunosuppressive genes, chemokines, and chemokine receptors.

Findings: CD161 was differentially expressed and predicted better survival status in most tumour types in TCGA. In addition, CD161 expression was significantly associated with immunoregulatory interactions between lymphoid and non-lymphoid cells. CD161 expression was closely correlated with T cell infiltration, immune checkpoints, immune activating genes, immunosuppressive genes, chemokines, and chemokine receptors.

Interpretation: Our results suggest that CD161 is a potential cancer biomarker. CD161 might synergize with other immune checkpoints to regulate the immune microenvironment, which could be applied in the development of new-targeted drugs for immunotherapy.

Funding: This work was supported by the National Nature Science Foundation of China (grant numbers 81773008, 81672756, 81872399, 81972897), the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2015), the Natural Science Foundation of Guangdong Province (grant number 2017A030311023), the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program: 2017BT01S131 and the Guangzhou Technology Project (grant number 201804010044), National Key R&D Program of China (Grant Nos. 2020YFC2006400), Key-Area Research and Development Program of Guangdong Province (2019B020227004).
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http://dx.doi.org/10.3389/fimmu.2021.688215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299557PMC
July 2021

NLRP1 in Bone Marrow Microenvironment Controls Hematopoietic Reconstitution After Transplantation.

Transplant Cell Ther 2021 Jul 21. Epub 2021 Jul 21.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China; Key Laboratory of Bone Marrow Stem Cell, Xuzhou, Jiangsu, China. Electronic address:

Pretreatment before transplantation initiates an inflammatory response. Inflammasomes are key regulators of immune and inflammatory responses, but their role in regulating hematopoiesis is unclear. Our study intended to assess the role and mechanism of nucleotide-binding domain and leucine-rich repeat pyrin-domain containing protein 1 (NLRP1) in the bone marrow microenvironment on hematopoiesis regulation. To explore the effects of an absence of NLRP1 on hematopoietic reconstitution, we established a hematopoietic cell transplantation model by infusing bone marrow mononuclear cells of wild-type C57BL/6 mice into either NLRP1 knockout (NLRP1-KO) or wild-type C57BL/6 mice. Using the transplantation model, the role of NLRP1 in the bone marrow microenvironment was determined by flow cytometry, hemacytometry, and hematoxylin and eosin staining. As the major component of the bone marrow microenvironment, mesenchymal stem cells (MSCs) were isolated to analyze the effects of NLRP1 on them by osteogenic and adipogenic induction. Endothelial cells (ECs) were isolated and sorted by magnetic beads. The expression of adhesion molecules and their relationship with nuclear factor kappa B (NF-κB) were measured by immunofluorescence, enzyme-linked immunosorbent assay, and western blot. Finally, the effect of NLRP1-deleted MSCs or ECs on hematopoietic stem and progenitor cells (HSPCs) was examined by establishing co-culture models. Compared with C57BL/6 recipients, reduced inflammatory cell infiltration, decreased levels of proinflammatory cytokines interleukin (IL)-18, IL-1β, IL-6, tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ), together with reduced pathological injury of bone marrow, were observed in NLRP1-KO recipients after transplantation. However, increased HSPC engraftment and hematopoietic reconstitution were detected in NLRP1-KO recipients after transplantation. Furthermore, MSCs isolated from NLRP1-KO mice had decreased osteogenic and adipogenic differentiation and increased proliferation and differentiation of HSPCs. The expression of adhesion molecules in ECs from NLRP1-KO mice was increased due to the promotion of nuclear translocation of NF-κB; these adhesion molecules are critical for hematopoietic stem cell homing. Knockout of NLRP1 in the bone marrow microenvironment could significantly relieve bone marrow inflammatory response and promote hematopoietic reconstitution, perhaps by regulating MSCs and ECs, indicating that NLRP1 might be a target for the treatment of delayed hematopoietic and immune recovery in patients after hematopoietic stem cell transplantation.
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http://dx.doi.org/10.1016/j.jtct.2021.07.016DOI Listing
July 2021

Steel Corrosion Behavior of Reinforced Calcium Aluminate Cement-Mineral Additions Modified Mortar.

Materials (Basel) 2021 Jul 20;14(14). Epub 2021 Jul 20.

School of Materials Science and Engineering, Tongji University, Shanghai 201804, China.

Mineral additions can eliminate the conversion in calcium aluminate hydrates and thus inhibit the future strength retraction of calcium aluminate cement (CAC). However, the impacts of these additions on the protection capacity of CAC concrete in relation to the corrosion of embedded steel reinforcement remains unclear. This paper focused on the corrosion behavior of steel reinforcement in slag, limestone powder, or calcium nitrate-modified CAC mortars via XRD and electrochemical methods (corrosion potential, electrochemical impedance, and linear polarization evaluation). The results indicate that strätlingite (CASH), which is formed in slag-modified CAC, has poor chloride-binding ability, leading to decline in corrosion resistance of the steel reinforcement. The electrochemical parameters of specimens immersed in NaCl solution suddenly drop at 14 days, which is 28 days earlier than that of the references. In contrast, the Ca[Al(OH)]0.5COOH·HO (CaAl·CO-LDH) and 3CaO·AlO·Ca(NO)·12HO (NO-AFm) in limestone powder and calcium nitrate-modified CAC mortar show great chloride-binding ability, thereby improving the corrosion resistance of the steel reinforcement. The electrochemical parameters of specimens modified with calcium nitrate maintain a slow decreasing trend within 90 days.
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http://dx.doi.org/10.3390/ma14144053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306743PMC
July 2021

Gamma Radiation Induce Inflammasome Signaling and Pyroptosis in Microvascular Endothelial Cells.

J Inflamm Res 2021 15;14:3277-3288. Epub 2021 Jul 15.

Department of Blood Diseases Institute, Xuzhou Medical University, Xuzhou City, 221002, Jiangsu Province, People's Republic of China.

Introduction: The extend to the clinical benefit of radiation therapy is the inability to eliminate only cancer cells and destroy normal cells such as microvascular endothelial in the vascular niche and turn induced-inflammasome signaling and cell death. These unfortunate injuries generated by ionizing radiation alter the therapeutic window and result in the re-occurrence of the malignancy. Therefore, we engaged in vitro studies by demonstrating radiation-induced inflammasome and cell death in endothelial cells.

Methods: The microvascular endothelial cells were cultured in a sterile dish, then kept in a humidifier of 5% at 37°C for 12 hours/more to attain confluence, and exposed at a dose of 1.8Gy/min achieve the coveted amounts except for the control. The cells were harvested 24 hours post-irradiation.

Results: Our findings indicate that gamma radiation activates the NOD-like receptor (NLR) family of NLRP1 and NLRP3 complex in microvascular endothelial cells. These complexes activate the inactive precursor of caspase-1, which cleaved to bioactive caspase -1 and enhances the production of pro-inflammatory cytokines of interleukin-1β and interleukin-18 that induce the dependent pyroptotic, which results in the production of chemokines, tumor necrosis factor-alpha (TNF-α), and high-mobility group protein-1 (HMGB-1). We also discovered the radiation could directly prompt caspase -1, which auto-cleaved to activate gasdermin D to potentiate pyroptosis independently.

Discussion: Overall, these findings suggested that reducing the unfavorable effect of radiation injuries could be challenging since gamma radiation induces the microvascular endothelial cells to cell death and activates the inflammasome signaling via different pathways.
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http://dx.doi.org/10.2147/JIR.S318812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289370PMC
July 2021

Association of FOXO3a gene polymorphisms and ankylosing spondylitis susceptibility in Eastern Chinese Han population.

Gene 2021 Oct 16;800:145832. Epub 2021 Jul 16.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Objective: To investigate the association of FOXO3a polymorphisms and ankylosing spondylitis (AS) susceptibility in Eastern Chinese Han population.

Methods: FOXO3a polymorphisms rs12206094, rs12212067, rs2253310, rs3800232, and rs4946933 were genotyped in 650 AS patients and 646 controls by the improved Multiple Ligase Detection Reaction.

Results: The distribution of genotype in rs12212067 polymorphism was significantly different between AS patients and controls (P = 0.020), especially in male population (P = 0.009). There was significant difference of the genotype frequency distribution at rs3800232 between patients and controls in male population. The results of binary regression analysis showed that the rs12212067 GG genotype and rs3800232 TT genotype were obviously correlated with elevated AS risk, and the associations were still significant after being adjusted by age and gender (all P < 0.05). Interestingly, rs12212067 and rs3800232 genotypes were associated with disease activity of patients. Additionally, haplotype block rs12212067- rs3800232 (OR = 1.403, 95%CI = 1.011-1.949) was further shown to confer promoting effect on developing AS.

Conclusion: Among Eastern Chinese Han population, FOXO3a polymorphism rs12212067 and rs3800232 may contribute to increased risk of developing AS, but well-designed multicenter studies are needed to further confirm these preliminary findings in the future.
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http://dx.doi.org/10.1016/j.gene.2021.145832DOI Listing
October 2021

ROS is essential for initiation of energy deprivation-induced autophagy.

J Genet Genomics 2021 Jun 16;48(6):512-515. Epub 2021 Jun 16.

Department of Biochemistry, and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.05.005DOI Listing
June 2021

Solution-focused brief therapy for adolescent and young adult cancer patients in China: a pilot randomized controlled trial.

J Psychosoc Oncol 2021 Jul 7:1-18. Epub 2021 Jul 7.

Department of Medicine, University of Chicago, Chicago, IL, USA.

Objective: This pilot clinical trial investigated solution-focused brief therapy (SFBT) for psychological distress among adolescent and young adult (AYA) patients with cancer in China.

Methods: Fifty Chinese AYA patients diagnosed with cancer were randomized into the treatment group (SFBT) and control group (active control). Psychological distress was measured by the brief symptom inventory and hope was measured by the Herth-Hope-Index. Treatment effects were analyzed using analysis-of-covariance and between-group small-sample-size corrected Hedges' .

Results: The results indicated that SFBT resulted in a significant reduction in the psychological distress and improvement in hope of AYA patients with cancer. Analyses of the 4-week posttreatment score suggest the short-term sustainability of SFBT for psychological distress among AYAs diagnosed with cancer.

Conclusions And Implications: This study has demonstrated that SFBT's impact is statistically significant and clinically meaningful. The inclusion of positive emotions, i.e., hope, as part of the investigation also highlighted the significance of promoting positive emotions among AYA patients with cancer.
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http://dx.doi.org/10.1080/07347332.2021.1931627DOI Listing
July 2021

Impacts of Space Restriction on the Microstructure of Calcium Silicate Hydrate.

Materials (Basel) 2021 Jun 30;14(13). Epub 2021 Jun 30.

School of Materials Science and Engineering, Tongji University, 4800 Cao'an Road, Shanghai 201804, China.

The effect of hydration space on cement hydration is essential. After a few days, space restriction affects the hydration kinetics which dominate the expansion, shrinkage and creep of cement materials. The influence of space restriction on the hydration products of tricalcium silicate was studied in this paper. The microstructure, morphology and composition of calcium silicate hydrate (C-S-H) were explored from the perspective of a specific single micropore. A combination of Raman spectra, Fourier transform infrared spectra, scanning electron microscopy and energy dispersive X-ray spectroscopy were employed. The results show that space restriction affects the structure of the hydration products. The C-S-H formed in the micropores was mainly composed of Q silicate tetrahedra with a high degree of polymerization. The C-S-H formed under standard conditions with a water to cement ratio of 0.5 mostly existed as Q units. Space restriction during hydration is conducive to the formation of C-S-H with silica tetrahedra of a high polymerization degree, while the amount of water filling the micropore plays no obvious role on the polymeric structure of C-S-H during hydration.
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http://dx.doi.org/10.3390/ma14133645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269597PMC
June 2021

Cinnamic acid inhibits Zika virus by inhibiting RdRp activity.

Antiviral Res 2021 08 24;192:105117. Epub 2021 Jun 24.

Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address:

In recent years, Zika virus (ZIKV), which causes severe diseases such as congenital microcephaly and Guillain-Barré syndrome, bringing serious harm to humans, has spread throughout the world. However, there are currently no effective drugs against the virus, and the need to develop anti-ZIKV drugs is thus urgent. In this study, we evaluated the antiviral efficacy of cinnamic acid against ZIKV by using reverse transcription-quantitative real-time PCR (qRT-PCR), plaque--forming, immunofluorescence and Western blotting. Additionally, Cinnamic acid possessed anti-ZIKV properties against the post-entry stage of the ZIKV replication cycle, and inhibited RdRp activity. In vivo, we found that cinnamic acid reduced the mortality of mice, viral load in the blood and ZIKV protein levels in the brain. Based on our experiments, cinnamic acid was found to be a potential effective anti-ZIKV drug.
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http://dx.doi.org/10.1016/j.antiviral.2021.105117DOI Listing
August 2021

Machine-learning-based children's pathological gait classification with low-cost gait-recognition system.

Biomed Eng Online 2021 Jun 22;20(1):62. Epub 2021 Jun 22.

Ningbo Research Institute, Zhejiang University, Ningbo, 315100, China.

Background: Pathological gaits of children may lead to terrible diseases, such as osteoarthritis or scoliosis. By monitoring the gait pattern of a child, proper therapeutic measures can be recommended to avoid the terrible consequence. However, low-cost systems for pathological gait recognition of children automatically have not been on market yet. Our goal was to design a low-cost gait-recognition system for children with only pressure information.

Methods: In this study, we design a pathological gait-recognition system (PGRS) with an 8 × 8 pressure-sensor array. An intelligent gait-recognition method (IGRM) based on machine learning and pure plantar pressure information is also proposed in static and dynamic sections to realize high accuracy and good real-time performance. To verifying the recognition effect, a total of 17 children were recruited in the experiments wearing PGRS to recognize three pathological gaits (toe-in, toe-out, and flat) and normal gait. Children are asked to walk naturally on level ground in the dynamic section or stand naturally and comfortably in the static section. The evaluation of the performance of recognition results included stratified tenfold cross-validation with recall, precision, and a time cost as metrics.

Results: The experimental results show that all of the IGRMs have been identified with a practically applicable degree of average accuracy either in the dynamic or static section. Experimental results indicate that the IGRM has 92.41% and 97.79% intra-subject recognition accuracy, and 85.78% and 78.81% inter-subject recognition accuracy, respectively, in the static and dynamic sections. And we find methods in the static section have less recognition accuracy due to the unnatural gesture of children when standing.

Conclusions: In this study, a low-cost PGRS has been verified and realize feasibility, highly average precision, and good real-time performance of gait recognition. The experimental results reveal the potential for the computer supervision of non-pathological and pathological gaits in the plantar-pressure patterns of children and for providing feedback in the application of gait-abnormality rectification.
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http://dx.doi.org/10.1186/s12938-021-00898-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220846PMC
June 2021

Pre-emptive pharmacological inhibition of fatty acid-binding protein 4 attenuates kidney fibrosis by reprogramming tubular lipid metabolism.

Cell Death Dis 2021 06 3;12(6):572. Epub 2021 Jun 3.

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Kidney fibrosis is a hallmark of chronic kidney disease (CKD) progression that is caused by tubular injury and dysregulated lipid metabolism. Genetic abolition fatty acid-binding protein 4 (FABP4), a key lipid transporter, has been reported to suppress kidney interstitial fibrosis. However, the role and underlying mechanism of chemical inhibition of FABP4 in fibrotic kidney have not been well-documented. Here, we examined preemptive the effect of a FABP4 inhibitor, BMS309403, on lipid metabolism of tubular epithelial cells (TECs) and progression of kidney fibrosis. The expression of FABP4 was significantly elevated, concomitated with the accumulation of lipid droplets in TECs during kidney fibrosis. Treatment with BMS309403 alleviated lipid deposition of TECs, as well as interstitial fibrotic responses both in unilateral ureteral obstruction (UUO)-engaged mice and TGF-β-induced TECs. Moreover, BMS309403 administration enhanced fatty acid oxidation (FAO) in TECs by regulating peroxisome proliferator-activated receptor γ (PPARγ) and restoring FAO-related enzyme activities; In addition, BMS309403 markedly reduced cell lipotoxicity, such as endoplasmic reticulum (ER) stress and apoptosis in fibrotic kidney. Taken together, our results suggest that preemptive pharmacological inhibition of FABP4 by BMS309403 rebalances abnormal lipid metabolism in TECs and attenuates the progression of kidney fibrosis, thus may hold therapeutic potential for the treatment of fibrotic kidney diseases.
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http://dx.doi.org/10.1038/s41419-021-03850-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175732PMC
June 2021

Rational Construction of Ruthenium-Cobalt Oxides Heterostructure in ZIFs-Derived Double-Shelled Hollow Polyhedrons for Efficient Hydrogen Evolution Reaction.

Small 2021 Jul 2;17(26):e2100998. Epub 2021 Jun 2.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

Transition metal oxides (TMOs) and their heterostructure hybrids have emerged as promising candidates for hydrogen evolution reaction (HER) electrocatalysts based on the recent technological breakthroughs and significant advances. Herein, Ru-Co oxides/Co O double-shelled hollow polyhedrons (RCO/Co O -350 DSHPs) with Ru-Co oxides as an outer shell and Co O as an inner shell by pyrolysis of core-shelled structured RuCo(OH) @zeolitic-imidazolate-framework-67 derivate at 350 °C are constructed. The unique double-shelled hollow structure provides the large active surface area with rich exposure spaces for the penetration/diffusion of active species and the heterogeneous interface in Ru-Co oxides benefits the electron transfer, simultaneously accelerating the surface electrochemical reactions during HER process. The theory computation further indicates that the existence of heterointerface in RCO/Co O -350 DSHPs optimize the electronic configuration and further weaken the energy barrier in the HER process, promoting the catalytic activity. As a result, the obtained RCO/Co O -350 DSHPs exhibit outstanding HER performance with a low overpotential of 21 mV at 10 mA cm , small Tafel slope of 67 mV dec , and robust stability in 1.0 m KOH. This strategy opens new avenues for designing TMOs with the special structure in electrochemical applications.
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http://dx.doi.org/10.1002/smll.202100998DOI Listing
July 2021

CCDC137 Is a Prognostic Biomarker and Correlates With Immunosuppressive Tumor Microenvironment Based on Pan-Cancer Analysis.

Front Mol Biosci 2021 13;8:674863. Epub 2021 May 13.

Dongguan Key Laboratory of Environmental Medicine, Department of Environmental and Occupational Health, School of Public Health, Guangdong Medical University, Dongguan, China.

Background: The coiled-coil domain containing (CCDC) family proteins have important biological functions in various diseases. However, the coiled-coil domain containing 137 (CCDC137) was rarely studied. We aim to investigate the role of CCDC137 in pan-cancer.

Methods: CCDC137 expression was evaluated in RNA sequence expression profilers of pan-cancer and normal tissues from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) database. The influence of CCDC137 on the prognosis of tumor patients was analyzed using clinical survival data from TCGA. Function and pathway enrichment analysis was performed to explore the role of CCDC137 using the R package "clusterProfiler." We further analyzed the correlation of immune cell infiltration score of TCGA samples and CCDC137 expression using TIMER2 online database.

Results: CCDC137 was over-expressed and associated with worse survival status in various tumor types. CCDC137 expression was positively correlated with tumor associated macrophages (TAMs) and cancer associated fibroblasts (CAFs) in Lower Grade Glioma (LGG) and Uveal Melanoma (UVM). In addition, high CCDC137 expression was positively correlated with most immunosuppressive genes, including TGFB1, PD-L1, and IL10RB in LGG and UVM.

Conclusions: Our study identified CCDC137 as an oncogene and predictor of worse survival in most tumor types. High CCDC137 may contribute to elevated infiltration of TAMs and CAFs and be associated with tumor immunosuppressive status.
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http://dx.doi.org/10.3389/fmolb.2021.674863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155610PMC
May 2021

Integrated microneedle-smartphone nucleic acid amplification platform for in-field diagnosis of plant diseases.

Biosens Bioelectron 2021 Sep 8;187:113312. Epub 2021 May 8.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27696, USA; Emerging Plant Disease and Global Food Security Cluster, North Carolina State University, Raleigh, NC, 27696, USA. Electronic address:

We demonstrate an integrated microneedle (MN)-smartphone nucleic acid amplification platform for "sample-to-answer" diagnosis of multiplexed plant pathogens within 30 min. This portable system consists of a polymeric MN patch for rapid nucleic acid extraction within a minute and a 3D-printed smartphone imaging device for loop-mediated isothermal amplification (LAMP) reaction and detection. We expanded the extraction of the MN technology for DNA targets as in the previous study (ACS Nano, 2019, 13, 6540-6549) to more fragile RNA biomarkers, evaluated the storability of the extracted nucleic acid samples on MN surfaces, and developed a smartphone-based LAMP amplification and fluorescent reader device that can quantify four LAMP reactions on the same chip. In addition, we have found that the MN patch containing as few as a single needle tip successfully extracted enough RNA for RT-PCR or RT-LAMP analysis. Moreover, MN-extracted RNA samples remained stable on MN surfaces for up to three days. The MN-smartphone platform has been used to detect both Phytophthora infestans DNA and tomato spotted wilt virus (TSWV) RNA down to 1 pg, comparable to the results from a benchtop thermal cycler. Finally, multiplexed detection of P. infestans and TSWV through a single extraction from infected tomato leaves and amplification on the smartphone without benchtop equipment was demonstrated.
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http://dx.doi.org/10.1016/j.bios.2021.113312DOI Listing
September 2021

Association of 5-Hydroxytryptamine 3 Receptor Antagonists With the Prognosis of Liver Failure.

Front Pharmacol 2021 22;12:648736. Epub 2021 Apr 22.

Department of Infectious Diseases, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Liver failure is a severe clinical syndrome with high mortality. 5-Hydroxytryptamine 3 receptor antagonists (5-HT3RAs) can reduce liver damage in animal models. We investigated whether 5-HT3RAs may improve the prognosis of liver failure. We analyzed the 28 and 90 days mortality of liver failure patients in relation to the use of 5-HT3RAs using data from a tertiary hospital in northwest China. According to the use of 5-HT3RAs, 419 patients with liver failure (46 acute, 93 sub-acute, 44 chronic, 236 acute on chronic) were divided into 5-HT3RA group ( = 105) and control group ( = 314). 5-HT3RAs were associated with decreased 28 days (HR 0.18, 95% CI 0.10-0.34, < 0.001) and 90 days (HR 0.21, 95% CI 0.13-0.33, 0.001) mortality. After propensity score matching (PSM) ( = 67 in each group), 5-HT3RAs were still significantly associated with reduced 28 days (HR 0.10, 95%CI 0.04-0.26, < 0.001) and 90 days (HR 0.16, 95%CI 0.08-0.31, < 0.001) mortality. 5-HT3RA group patients had significantly higher 28 and 90 days survivals than controls both before and after PSM (all < 0.001). This study shows that 5-HT3RAs are associated with increased survival of liver failure patients and thus may be used to treat liver failure if the findings are confirmed by additional studies.
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http://dx.doi.org/10.3389/fphar.2021.648736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100675PMC
April 2021

Lung stem cells in regeneration and tumorigenesis.

J Genet Genomics 2021 04 17;48(4):268-276. Epub 2021 Mar 17.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Pulmonary Hospital, Tongji University, Shanghai 200092, China. Electronic address:

Adult lung is a highly quiescent organ, with extremely low cell turnover frequency. However, emerging evidences support the occurrence of repair and regeneration in pulmonary epithelia in response to various injuries. Lung regeneration mainly depends on the proliferation of regionally distributed pulmonary stem cells that re-enter the cell cycle. Genetic lineage-tracing approaches help to track the lung epithelial differentiation and/or de-differentiation path, and single-cell transcriptomic technique reveals the essential molecular signaling involved in lung regeneration. Dysregulation of the molecular signaling that balances quiescence and self-renewal leads to the transformation of lung stem cells, and thus promotes lung cancer development. Interestingly, different subtypes of lung cancer share common cells of origin and the pathological transition among various subtypes is responsible for drug resistance in the clinic. In this review, we summarize the recent understanding of lung stem cells in regeneration and tumorigenesis as well as related molecular mechanisms, with the hope to provide helpful insights for clinical treatments of respiratory diseases.
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http://dx.doi.org/10.1016/j.jgg.2020.12.004DOI Listing
April 2021

Protein tyrosine phosphatase receptor type D gene promotes radiosensitivity via STAT3 dephosphorylation in nasopharyngeal carcinoma.

Oncogene 2021 Apr 6;40(17):3101-3117. Epub 2021 Apr 6.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Radiotherapy is essential to the treatment of nasopharyngeal carcinoma (NPC) and acquired or innate resistance to this therapeutic modality is a major clinical problem. However, the underlying molecular mechanisms in the radiation resistance in NPC are not fully understood. Here, we reanalyzed the microarray data from public databases and identified the protein tyrosine phosphatase receptor type D (PTPRD) as a candidate gene. We found that PTPRD was downregulated in clinical NPC tissues and NPC cell lines with its promoter hypermethylated. Functional assays revealed that PTPRD overexpression sensitized NPC to radiation in vitro and in vivo. Importantly, miR-454-3p directly targets PTPRD to inhibit its expression and biological effect. Interestingly, mechanistic analyses indicate that PTPRD directly dephosphorylates STAT3 to enhance Autophagy-Related 5 (ATG5) transcription, resulting in triggering radiation-induced autophagy. The immunohistochemical staining of 107 NPC revealed that low PTPRD and high p-STAT3 levels predicted poor clinical outcome. Overall, we showed that PTPRD promotes radiosensitivity by triggering radiation-induced autophagy via the dephosphorylation of STAT3, thus providing a potentially useful predictive biomarker for NPC radiosensitivity and drug target for NPC radiosensitization.
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http://dx.doi.org/10.1038/s41388-021-01768-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084736PMC
April 2021

Sustainable development of microalgal biotechnology in coastal zone for aquaculture and food.

Sci Total Environ 2021 Aug 10;780:146369. Epub 2021 Mar 10.

State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou, Hainan 570228, China. Electronic address:

Region-specific Research and Development (R&D) of microalga-derived product systems are crucial if "biotech's green gold" is to be explored in a rational and economically viable way. Coastal zones, particularly the locations around the equator, are typically considered to be optimum cultivation sites due to stable annual temperature, light, and ready availability of seawater. However, a 'cradle-to-grave' assessment of the development of microalgal biotechnology in these areas, not only under the laboratory conditions, but also in the fields has not yet been demonstrated. In this study, to evaluate the viability of microalga-derived multi-product technology, we showed the development of microalgal biotechnology in coastal zones for aquaculture and food. By creating and screening a (sub)tropical microalgal collection, a Chlorella strain MEM25 with a robust growth in a wide range of salinities, temperatures, and light intensities was identified. Evaluation of the economic viability and performance of different scale cultivation system designs (500 L and 5000 L closed photobioreactors and 60,000 L open race ponds, ORPs) at coastal zones under geographically specific conditions showed the stable and robust characteristics of MEM25 across different production system designs and various spatial and temporal scales. It produces high amounts of proteins and polyunsaturated fatty acids (PUFAs) in various conditions. Feeding experiments reveal the nutritional merits of MEM25 as food additives where PUFAs and essential amino acids are enriched and the algal diet improves consumers' growth. Economic evaluation highlights an appreciable profitability of MEM25 production as human or animal food using ORP systems. Therefore, despite the pros and cons, sound opportunities exist for the development of market-ready multiple-product systems by employing region-specific R&D strategies for microalgal biotechnology.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146369DOI Listing
August 2021

Short-term effects of ambient temperature and pollutants on the mortality of respiratory diseases: A time-series analysis in Hefei, China.

Ecotoxicol Environ Saf 2021 Jun 24;215:112160. Epub 2021 Mar 24.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Background: The air pollution has become an important environmental health problem due to its adverse health effect. The objective of this study was to investigate the effects of ambient temperature and pollutants on mortality of respiratory diseases (RD) in Hefei, China, a typical inland city.

Methods: Nonlinear exposure-response dependencies and delayed effects of urban daily mean temperature (DMT) and pollutants were evaluated by distributed lag non-linear models (DLNM). To further explore this effect, different genders and ages were also examined by stratified analysis.

Results: A total of 12876 deaths from RD were collected from January 1, 2014 to December 31, 2018 in Hefei, China. There was a U-shaped correlation between DMT and RD mortality, and the RD mortality rised by 11.6% (95% CI: 2.2-22.0%) when the DMT was 35.8 °C (reference temperature is 20 °C). The results show that risk of death with short-term exposure to elevated concentrations of PM and SO was not significant. The maximum hysteresis and cumulative relative risk (RR) of RD mortality were 1.012 (95% CI: 1.003 ~ 1.021, lag 0 day) and 1.072 (95% CI: 1.014 ~1.133, lag 10 days) for each 10 μg/m augment in NO; 1.005 (95% CI: 1.001-1.009, lag 0 day) and 1.027 (95% CI: 1.004-1.051, lag 10 days) for each 10 μg/m augment in O a negative association between CO exposure and the cumulative risk of death was observed (RR = 0.964, 95% CI: 0.935-0.993, lag 07 days). Subgroup analysis showed the effect of high temperatures, NO O and CO exposure was still statistically significant for the elderly and male.

Conclusion: The present study found that short-term exposure to high temperature, NO O and CO were significantly associated with the risk of RD mortality and male as well as elderly are more susceptible to these factors.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112160DOI Listing
June 2021

Prediction of Soil Clay Content and Cation Exchange Capacity Using Visible Near-Infrared Spectroscopy, Portable X-ray Fluorescence, and X-ray Diffraction Techniques.

Environ Sci Technol 2021 04 22;55(8):4629-4637. Epub 2021 Mar 22.

School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Eveleigh, NSW 2015, Australia.

This article investigates a novel data fusion method to predict clay content and cation exchange capacity using visible near-infrared (visNIR) spectroscopy, portable X-ray fluorescence (pXRF), and X-ray diffraction (XRD) techniques. A total of 367 soil samples from two study areas in regional Australia were analyzed and intra- and interarea calibration options were explored. Cubist models were constructed using information from each device independently and in combination. pXRF produced the most accurate predictions of any individual device. Models based on fused data significantly improved the accuracy of predictions compared with those based on individual devices. The combination of pXRF and visNIR had the greatest performance. Overall, the relative increase in Lin's concordance correlation coefficient ranged from 1% to 12% and the corresponding decrease in root-mean-square error (RMSE) ranged from 10% to 46%. Provision of XRD data resulted in a decrease in observed RMSE values, although differences were not significant. Validation metrics were less promising when models were calibrated in one study area and then transferred to the other. Observed RMSE values were ∼2 to 3 times larger under this model transfer scenario and independent use of XRD was found to have the best overall performance.
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http://dx.doi.org/10.1021/acs.est.0c04130DOI Listing
April 2021

Multi-modal magnetic resonance imaging-based grading analysis for gliomas by integrating radiomics and deep features.

Ann Transl Med 2021 Feb;9(4):298

School of Biomedical Engineering, Southern Medical University, Guangzhou, China.

Background: To investigate the feasibility of integrating global radiomics and local deep features based on multi-modal magnetic resonance imaging (MRI) for developing a noninvasive glioma grading model.

Methods: In this study, 567 patients [211 patients with glioblastomas (GBMs) and 356 patients with low-grade gliomas (LGGs)] between May 2006 and September 2018, were enrolled and divided into training (n=186), validation (n=47), and testing cohorts (n=334), respectively. All patients underwent postcontrast enhanced T1-weighted and T2 fluid-attenuated inversion recovery MRI scanning. Radiomics and deep features (trained by 8,510 3D patches) were extracted to quantify the global and local information of gliomas, respectively. A kernel fusion-based support vector machine (SVM) classifier was used to integrate these multi-modal features for grading gliomas. The performance of the grading model was assessed using the area under receiver operating curve (AUC), sensitivity, specificity, Delong test, and -test.

Results: The AUC, sensitivity, and specificity of the model based on combination of radiomics and deep features were 0.94 [95% confidence interval (CI): 0.85, 0.99], 86% (95% CI: 64%, 97%), and 92% (95% CI: 75%, 99%), respectively, for the validation cohort; and 0.88 (95% CI: 0.84, 0.91), 88% (95% CI: 80%, 93%), and 81% (95% CI: 76%, 86%), respectively, for the independent testing cohort from a local hospital. The developed model outperformed the models based only on either radiomics or deep features (Delong test, both of P<0.001), and was also comparable to the clinical radiologists.

Conclusions: This study demonstrated the feasibility of integrating multi-modal MRI radiomics and deep features to develop a promising noninvasive grading model for gliomas.
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http://dx.doi.org/10.21037/atm-20-4076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944310PMC
February 2021

Epstein-Barr virus (EBV) encoded microRNA BART8-3p drives radioresistance-associated metastasis in nasopharyngeal carcinoma.

J Cell Physiol 2021 Sep 10;236(9):6457-6471. Epub 2021 Mar 10.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Radiotherapy plays an important role in the treatment of nasopharyngeal carcinoma (NPC), however, 20% of patients with NPC exhibit unusual radioresistance. Patients with radioresistance are at risk of recurrence, so it is imperative to explore the mechanism of resistance to radiotherapy. In the past, studies on the mechanism of radioresistance have been restricted to DNA damage and related cell cycle remodeling or apoptosis. So far, no studies have explored the relationship between radioresistance and metastasis. Through the analysis of clinical samples, we observed that the metastasis rate of recurrent NPC was much higher than that of primary patients. In vitro and in vivo experiments showed that NPC cells with acquired radioresistance exhibited a stronger ability for invasion and metastasis. Mechanistically, we found that the Epstein-Barr virus (EBV)-encoded miRNA BART8-3p was increased in patients with NPC, and its expression was positively correlated with adverse prognostic factors, such as radioresistance. Besides this, miR-BART8-3p promoted the epithelial-mesenchymal transition, invasion, and metastasis of radioresistant NPC cells by targeting and inhibiting their PAG1 host gene. These findings suggested a novel role for EBV-miR-BART8-3p in promoting NPC radioresistance-associated metastasis and highlighted its potential value as a prognostic indicator or therapeutic target.
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http://dx.doi.org/10.1002/jcp.30320DOI Listing
September 2021

Epigenetics of ankylosing spondylitis: Recent developments.

Int J Rheum Dis 2021 Apr 19;24(4):487-493. Epub 2021 Feb 19.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China.

Ankylosing spondylitis (AS) is a chronic inflammatory autoimmune disease which mainly affects the spine, sacroiliac joint and peripheral joints. To date, the exact causes and pathogenesis of AS still remain unknown. It is considered that the pathogenesis of AS is associated with genetic, infection, environment, immunity and other factors. Among them, the role of genetic factors in the pathogenesis of AS has been studied most deeply. However, over the past few years, the function of environmental predisposition and epigenetic modification in the pathogenesis of AS has received extensive attention. This paper summarizes the recent progress in the epigenetics of AS, including abnormal epigenetic modifications at AS-associated genomic loci, such as DNA methylation, histone modification, microRNA, and so on. In summary, the findings of this review attempt to explain the role of epigenetic modification in the occurrence and development of AS. Nevertheless, there are still unknown and complicated aspects worth exploring to deepen our understanding of the pathogenesis of AS.
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http://dx.doi.org/10.1111/1756-185X.14080DOI Listing
April 2021

Role of Forkhead box O3a transcription factor in autoimmune diseases.

Int Immunopharmacol 2021 Mar 4;92:107338. Epub 2021 Jan 4.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Forkhead box O3a (FOXO3a) transcription factor, the most important member of Forkhead box O family, is closely related to cell proliferation, apoptosis, autophagy, oxidative stress and aging. The downregulation of FOXO3a has been verified to be associated with the poor prognosis, severer malignancy and chemoresistance in several human cancers. The activity of FOXO3a mainly regulated by phosphorylation of protein kinase B. FOXO3a plays a vital role in promoting the apoptosis of immune cells. FOXO3a could also modulate the activation, differentiation and function of T cells, regulate the proliferation and function of B cells, and mediate dendritic cells tolerance and immunity. FOXO3a accommodates the immune response through targeting nuclear factor kappa-B and FOXP3, as well as regulating the expression of cytokines. Besides, FOXO3a participates in intercellular interactions. FOXO3a inhibits dendritic cells from producing interleukin-6, which inhibits B-cell lymphoma-2 (BCL-2) and BCL-XL expression, thereby sparing resting T cells from apoptosis and increasing the survival of antigen-stimulated T cells. Recently, plentiful evidences further illustrated the significance of FOXO3a in the pathogenesis of autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, inflammatory bowel disease, ankylosing spondylitis, myositis, multiple sclerosis, and systemic sclerosis. In this review, we focused on the biological function of FOXO3a and related signaling pathways regarding immune system, and summarized the potential role of FOXO3a in the pathogenesis, progress and therapeutic potential of autoimmune diseases.
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http://dx.doi.org/10.1016/j.intimp.2020.107338DOI Listing
March 2021

PremPS: Predicting the impact of missense mutations on protein stability.

PLoS Comput Biol 2020 12 30;16(12):e1008543. Epub 2020 Dec 30.

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China.

Computational methods that predict protein stability changes induced by missense mutations have made a lot of progress over the past decades. Most of the available methods however have very limited accuracy in predicting stabilizing mutations because existing experimental sets are dominated by mutations reducing protein stability. Moreover, few approaches could consistently perform well across different test cases. To address these issues, we developed a new computational method PremPS to more accurately evaluate the effects of missense mutations on protein stability. The PremPS method is composed of only ten evolutionary- and structure-based features and parameterized on a balanced dataset with an equal number of stabilizing and destabilizing mutations. A comprehensive comparison of the predictive performance of PremPS with other available methods on nine benchmark datasets confirms that our approach consistently outperforms other methods and shows considerable improvement in estimating the impacts of stabilizing mutations. A protein could have multiple structures available, and if another structure of the same protein is used, the predicted change in stability for structure-based methods might be different. Thus, we further estimated the impact of using different structures on prediction accuracy, and demonstrate that our method performs well across different types of structures except for low-resolution structures and models built based on templates with low sequence identity. PremPS can be used for finding functionally important variants, revealing the molecular mechanisms of functional influences and protein design. PremPS is freely available at https://lilab.jysw.suda.edu.cn/research/PremPS/, which allows to do large-scale mutational scanning and takes about four minutes to perform calculations for a single mutation per protein with ~ 300 residues and requires ~ 0.4 seconds for each additional mutation.
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http://dx.doi.org/10.1371/journal.pcbi.1008543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802934PMC
December 2020

Anti-CD74 antibodies in spondyloarthritis: A systematic review and meta-analysis.

Semin Arthritis Rheum 2021 02 10;51(1):7-14. Epub 2020 Dec 10.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Objective: There is still an unmet need for a simple and reliable biomarker for the diagnosis of spondyloarthritis. Recent studies indicated that anti-CD74 antibody could act as a biomarker for spondyloarthritis. Therefore, this review aims to evaluate the levels of anti-CD74 IgG and IgA antibodies in spondyloarthritis and the diagnostic value of anti-CD74 antibodies.

Methods: PubMed, Web of Science and Medline were comprehensively searched from inception to August 7th, 2019. The pooled standard mean difference (SMD) with 95% confidence interval (CI) was used to estimate the differences of the levels of anti-CD74 IgG and IgA antibodies between spondyloarthritis patients and controls. Sensitivity, specificity and summary receiver operating characteristics (SROC) curve were used for evaluating the diagnostic value of anti-CD74 antibodies. The use of fixed-effect or random-effects model depended on heterogeneity.

Results: Among 55 searched studies, 9 studies were finally included for analysis. Anti-CD74 IgG and IgA antibodies were both significantly increased in spondyloarthritis patients compared with matched controls (IgG: SMD = 0.88, 95% CI = 0.55 to 1.21; IgA: SMD = 0.98, 95% CI = 0.68 to 1.28). The pooled sensitivity, specificity and area under the SROC curve of anti-CD74 IgG antibodies were 0.61, 0.90 and 0.8881, while these indicators of anti-CD74 IgA antibodies were 0.59, 0.95 and 0.8671, respectively.

Conclusion: Anti-CD74 IgG and IgA antibodies were significantly increased in spondyloarthritis patients and suggest a high diagnostic specificity of spondyloarthritis. Anti-CD74 antibody could potentially be a biomarker for the diagnosis of spondyloarthritis, but many open questions remain.
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http://dx.doi.org/10.1016/j.semarthrit.2020.12.002DOI Listing
February 2021
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