Publications by authors named "Chen Yuan"

1,596 Publications

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Clinical study on acupuncture treatment of gastrointestinal damp-heat acne.

Medicine (Baltimore) 2021 Nov;100(44):e27503

School of Acupuncture-Moxibustion and Tuina, Henan University of Chinese Medicine, 63 Dongming Road, Jinshui District, Henan, China.

Background: Acne is a common inflammatory disease of sebaceous glands, which brings extensive emotional and psychological distress to patients. Although acupuncture has certain advantages in the treatment of acne, the curative effect is not exact. The purpose of this trial is to evaluate the feasibility, preliminary efficacy, and safety of the "Spleen and Stomach Guiyuan Acupuncture Method" (SSGA) in the treatment of gastrointestinal damp-heat acne.

Methods: The proposed protocol is planned as a randomized, assessor-blind, conventional-treatment-controlled trial to evaluate the efficacy of SSGA on gastrointestinal damp-heat acne. Seventy six gastrointestinal damp-heat acne patients will be randomly divided into 2 groups and receive SSGA or conventional acupuncture treatment. The entire study period is 12 weeks, including an 8-week treatment period and a 4-week follow-up period. All patients will receive 16 sessions of acupuncture treatment over 8 weeks. The primary outcome is the investigation global assessment (IGA) at week 8, which is an overall assessment of the degree of the inflammatory and non-inflammatory lesion. The secondary outcomes include IGA, the total facial lesion count (Total Lesion Count), the acne-specific quality of life, etc at weeks 8 and 12. The Expectation and Credibility of treatment rating scale will be used to measure the patients' attitudes to acupuncture after the first treatment. Adverse events will also be recorded.

Discussion: This study is helpful to evaluate the feasibility, preliminary efficacy, and safety of SSGA in the treatment of gastrointestinal damp-heat acne. The results will be used in sample size calculations for subsequent large-scale studies.Trial registration: Chinese Clinical Trial Registry ChiCTR2100047363. Registered on June 13, 2021.
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http://dx.doi.org/10.1097/MD.0000000000027503DOI Listing
November 2021

Lignocellulose nanocrystals from pineapple peel: Preparation, characterization and application as efficient Pickering emulsion stabilizers.

Food Res Int 2021 12 11;150(Pt A):110738. Epub 2021 Oct 11.

College of Food Science, Southwest University, Chongqing 400715, China. Electronic address:

In this study, the pineapple peel treated with different degrees of delignification was used to isolate lignocellulose nanocrystals (LCNC) by sulfuric acid hydrolysis. Controlling delignification treatments can adjust the morphology and structure of pineapple peel and the retention of lignin, thereby achieving the regulation of the properties of LCNC, such as morphology, crystallinity, hydrophobicity and rheological properties. The results of atomic force microscope (AFM), confocal laser scanning microscopy (CLSM), UV/visible (UV-Vis) spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the presence of lignin in LCNC, showing a rod-like structure with the distribution of lignin. Regulating delignification of pineapple peel can adjust the average length (310 ∼ 460 nm), diameter (19 ∼ 38 nm), crystallinity (61% ∼ 71%) and hydrophobicity (contact angle 84° ∼ 60°) of the obtained LCNC by acid hydrolysis, and influence the performance of its stabilized Pickering emulsions. This work confirms that the properties of LCNC can be controlled through adjusting delignification degree, possessing great significance for the high value utilization of lignocellulosic agricultural waste.
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http://dx.doi.org/10.1016/j.foodres.2021.110738DOI Listing
December 2021

A high-resolution trajectory data driven method for real-time evaluation of traffic safety.

Accid Anal Prev 2021 Dec 1:106503. Epub 2021 Dec 1.

School of Traffic and Transportation Engineering, Central South University, Changsha, Hunan, 410075, PR China.

Real-time safety evaluation is essential for developing proactive safety management strategy and improving the overall traffic safety. This paper proposes a method for real-time evaluation of road safety, in which traffic states and conflicts are combined to explore the internal relationship based on high-resolution trajectory data. In order to assess the real-time traffic safety at a lane level, the trajectory data of the HighD dataset from Germany are utilized to collect lane-based dataset. A surrogate safety measure, time-to-collision (TTC) index, is used for the conflict identification. A binary logistic regression model is employed to quantify the relationship between traffic states and conflicts. Moreover, machine learning methods, including support vector machine, decision tree, random forest, and gradient boosting decision tree, are applied for real-time evaluation. A total of 24 models are trained using the selected four classifier algorithms, and random forest achieves the best performance with 0.85 of the overall accuracy. The results show that the conflict risk can be well estimated by the proposed method. The findings of this study contribute to the high-precision evaluation of real-time traffic safety and the development of proactive safety management.
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http://dx.doi.org/10.1016/j.aap.2021.106503DOI Listing
December 2021

Learning Deep Blind Quality Assessment for Cartoon Images.

IEEE Trans Neural Netw Learn Syst 2021 Nov 30;PP. Epub 2021 Nov 30.

Although the cartoon industry has developed rapidly in recent years, few studies pay special attention to cartoon image quality assessment (IQA). Unfortunately, applying blind natural IQA algorithms directly to cartoons often leads to inconsistent results with subjective visual perception. Hence, this brief proposes a blind cartoon IQA method based on convolutional neural networks (CNNs). Note that training a robust CNN depends on manually labeled training sets. However, for a large number of cartoon images, it is very time-consuming and costly to manually generate enough mean opinion scores (MOSs). Therefore, this brief first proposes a full reference (FR) cartoon IQA metric based on cartoon-texture decomposition and then uses the estimated FR index to guide the no-reference IQA network. Moreover, in order to improve the robustness of the proposed network, a large-scale dataset is established in the training stage, and a stochastic degradation strategy is presented, which randomly implements different degradations with random parameters. Experimental results on both synthetic and real-world cartoon image datasets demonstrate the effectiveness and robustness of the proposed method.
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http://dx.doi.org/10.1109/TNNLS.2021.3127720DOI Listing
November 2021

Study on the Correlation Between Ischemic Leukoaraiosis and Cerebral Large Artery Stenosis Using the Stages of the Preinfarction Period Based on the Result of Computed Tomography Perfusion.

Neurologist 2021 Nov 26. Epub 2021 Nov 26.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, Suzhou Department of Neurology, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.

Background And Objective: The influence of cerebral large artery stenosis (CLAS) on ischemic leukoaraiosis (LA) remains elusive. Based on the proposed stages of the preinfarction period, this study aimed to adopt the staging system to assess the correlation between ischemic LA and CLAS.

Materials And Methods: Patients with unilateral CLAS ≥50% and without cerebral stroke were screened. The severity and distribution of stenosis were evaluated on computed tomography angiography images. The degree of regional cerebral perfusion was rated according to the stages of preinfarction period: 0=normal, 1=stage Ia, 2=stage Ib, 3=stage IIa, 4=stage IIb. Stage I included stage Ia and stage Ib. Stage II included stage IIa and stage IIb. LA was scored with Fazakas scale on T2-weighted image and/or fluid-attenuated inversion recovery sequences.

Results: The cohort consisted of 212 patients (mean age, 66.89±11.39 y), including 145 (68.40%) males. CLAS severity and distribution did not differ between patients with and without LA (P>0.05). Normal, stage I, and stage II had significantly different incidences of LA and hemispheric LA scores in the left and right hemispheres (P<0.05). The degree of regional cerebral perfusion was independently associated with LA in the left (P=0.0094) and right hemispheres (P=0.0091).

Conclusions: Ischemic LA is not directly related to CLAS but is independently associated with the degree of CLAS-induced cerebral hypoperfusion. The stages of the preinfarction period are helpful in identifying people at high risk of LA progression.
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http://dx.doi.org/10.1097/NRL.0000000000000350DOI Listing
November 2021

Avian leukosis virus subgroup J infection influences the gut microbiota composition in Huiyang bearded chickens.

Lett Appl Microbiol 2021 Nov 26. Epub 2021 Nov 26.

School of Life Science, Huizhou University, Huizhou, 516007, China.

Avian leukosis virus (ALV) poses a major threat to poultry. The chicken gut microbiota plays critical roles in host performance, health, and immunity. However, the effect of viral infection on the microbiota of Chinese local chickens is not well understood. In this study, we performed high-throughput 16S rRNA gene sequencing and evaluated the gut microbiota profiles using feces from ALV subgroup J (ALV-J)-infected and healthy Huiyang bearded chickens (Chinese local chickens). At the phylum level, ALV-J infection mainly increased the abundance of Bacteroidetes and Proteobacteria and decreased that of Firmicutes. An analysis at the order, family, and genus levels showed that the abundance of Lactobacillales, Lactobacillaceae, and Lactobacillus was the highest in normal chicken feces, accounting for 89.07%, 86.47%, and 86.46%, respectively, of phylotypes. Moreover, samples from ALV-J-infected chickens were enriched with Bacteroidales, Clostridiales, Bacteroidaceae, Ruminococcaceae, Lachnospiraceae, and Bacteroides. Our findings highlight that ALV-J infection alters the gut microbiota and disrupts the host-microbial homeostasis in chickens, which may be involved in the pathogenesis of ALV-J infection.
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http://dx.doi.org/10.1111/lam.13617DOI Listing
November 2021

Polarity control of DNA adsorption enabling the surface functionalization of CuO nanozymes for targeted tumor therapy.

Mater Horiz 2021 Mar 23;8(3):972-986. Epub 2020 Dec 23.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, China.

Nanomaterials with intrinsic catalytic activities (nanozyme) have drawn broad attention for various biomedical applications, with peroxidase-mimic nanozymes particularly attractive for cancer therapy due to their capability to catalyze the conversion of tumor-abundant HO into more toxic hydroxyl radicals (˙OH) for effective tumor ablation. However, the facile surface modification of nanozymes for tumor-targeted delivery while retaining their catalytic activity remains a challenge. Here, we report an approach to functionalize the CuO nanozyme with DNA to enable targeted delivery and selective tumor destruction. We systematically studied the adsorption of DNA on the CuO surface, with special attention paid to the catalytic activity and DNA adsorption stability in the presence of various biological ligands. After gaining a fundamental understanding, a di-block DNA sequence was designed for adsorption on to the CuO surface, which allowed stable adsorption during in vivo circulation, passive accumulation into the tumor tissue, and the specific recognition of tumor cells, resulting in significant nanocatalytic tumor suppression in tumor xenograft mice models with no noticeable cytotoxicity. This work paves a way for the rational design of DNA-modified nanozymes for catalytic tumor therapy, and fundamentally, provides a new insight into the biointerface chemistry of CuO with DNA.
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http://dx.doi.org/10.1039/d0mh01372bDOI Listing
March 2021

Silica covered stannic oxide nanoparticles - an easily prepared robust substrate for optical sensors.

Nanotechnology 2021 Nov 24. Epub 2021 Nov 24.

University of New Hampshire, 23 Academic way, Durham, New Hampshire, 03824, UNITED STATES.

This paper describes a facile way to prepare a photophysically inert sensor substrate. Stannic oxide encapsulated silica nanoparticles with average diameters between 30 and 70 nm have been prepared by one-pot reverse-phase emulsion methodology. The constituents and core/shell morphology of the nanoparticles were demonstrated by electron microscopic technology, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). X-ray diffraction (XRD) was employed to provide additional constitutional and structural information. It has been shown that nanoparticles prepared by this method are optically clear in suspension. After anchoring optical indicators, this nanoparticle can be utilized as a sensor module both in biology and other analytical areas.
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http://dx.doi.org/10.1088/1361-6528/ac3ce3DOI Listing
November 2021

Single Crystal Halide Perovskite Film for Nonlinear Resistive Memory with Ultrahigh Switching Ratio.

Small 2021 Nov 23:e2103881. Epub 2021 Nov 23.

College of Energy, Soochow Institute for Energy and Materials Innovations, Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province, Soochow University, Suzhou, 215000, China.

Morre's law is coming to an end only if the memory industry can keep stuffing the devices with new functionality. Halide perovskite acts as a promising candidate for application in next-generation nonvolatile memory. As is well known, the switching ratio is the key device requirement of resistive memory to improve recognition accuracy. Here, the authors introduce an all-inorganic halide perovskite CsPbBr single crystal film (SCF) into resistive memory as an active layer. The Ag/CsPbBr /Ag memory cells exhibit reproducible resistive switching with an ultrahigh switching ratio (over 10 ) and a fast switching speed (1.8 µs). It is studied that the Schottky barrier of metal/CsPbBr SCF contact follows the tendency of Schottky-Mott theory, and the Fermi level pinning effect is effectively reduced. The interface S parameter of metal/CsPbBr SCF contact is 0.50, suggesting a great interface contact is formed. The great interface contact contributes to the steady high resistance state (HRS), and then the steady HRS leads to an ultrahigh resistive switching ratio. This work demonstrates high performance from halide perovskite SCF-based memory. The introduction of halide perovskite SCF in resistive random access memory provides great potential as an alternative in future computing systems.
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http://dx.doi.org/10.1002/smll.202103881DOI Listing
November 2021

Enhanced Interface Properties and Stability of Lignocellulose Nanocrystals Stabilized Pickering Emulsions: The Leading Role of Tannic Acid.

J Agric Food Chem 2021 Nov 23. Epub 2021 Nov 23.

College of Food Science, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing 400715, China.

Cellulose and tannin are both abundant and biodegradable biopolymers, whose integrations show great potential in the food field due to their nutritional properties and biological activity. Here, lignocellulose nanocrystals (LCNC) isolated from pineapple peel were complexed with tannic acid (TA) through hydrogen-bonding interaction to prepare the LCNC/TA complex for stabilizing Pickering emulsions. Introducing TA decreased the interfacial tension (23.8-20.1 mN/m) and water contact angle (83.2-56.2°) with the LCNC/TA ratio ranging from 1:0 to 1:0.8 (w/w) but increased the size of the LCNC/TA complex. The droplet size of emulsions decreased from 115.0 to 51.3 μm accompanied by improved rheological properties. The emulsions stabilized by the LCNC/TA complex exhibited higher storage and environmental stabilities than those stabilized by LCNC alone. Interestingly, TA effectively promoted the interfacial adsorption of LCNC to build a stronger interfacial layer. The emulsion network structure was enhanced due to the formation of hydrogen-bonding interaction between LCNC and TA in the continuous phase.
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http://dx.doi.org/10.1021/acs.jafc.1c04930DOI Listing
November 2021

RPS4Y1 Promotes High Glucose-Induced Endothelial Cell Apoptosis and Inflammation by Activation of the p38 MAPK Signaling.

Diabetes Metab Syndr Obes 2021 12;14:4523-4534. Epub 2021 Nov 12.

Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, 100029, People's Republic of China.

Aim: Endothelial dysfunction is a key pathological basis for diabetes mellitus complications, including diabetic nephropathy, diabetic retinopathy, and diabetic cardiomyopathy. This study aimed to reveal the functional role of ribosomal protein S4 Y-linked 1 (RPS4Y1) in endothelial dysfunction.

Methods: Human umbilical vein endothelial cells (HUVECs) were subjected to high glucose. The expression of RPS4Y1 in cells was overexpressed or silenced by plasmid or siRNA transfection. MTT assay, flow cytometry, JC-1 probe, scratch test, tube formation, and ELISA were conducted to assess the effects of RPS4Y1 on cell. Western blot was performed to assay the downstream signaling of RPS4Y1. The inhibitors of p38, ERK, and Jnk were used to treat cells to validate the involvement of them in RPS4Y1-mediated endothelial dysfunction.

Results: RPS4Y1 was upregulated in HUVECs in response to high glucose in both dose- and time-dependent manners. Overexpression of RPS4Y1 induced viability loss, apoptosis, and inflammation, but inhibited cell migration and tube formation. Silence of RPS4Y1 impacted these aspects in a contrary trend. The phosphorylation of p38 rather than ERK and Jnk was activated by RPS4Y1. In addition, the dysfunction of HUVECs mediated by RPS4Y1 was attenuated by SB203580 (a specific inhibitor of p38 signaling).

Conclusion: The highly expressed RPS4Y1 in endothelial cells may contribute to high glucose-induced dysfunction through regulating p38 MAPK signaling. RPS4Y1 might be a potential therapeutic target for treating diabetes mellitus complications.
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http://dx.doi.org/10.2147/DMSO.S329209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594791PMC
November 2021

β-Alanine-Anchored SnO Inducing Facet Orientation for High-Efficiency Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Nov 20. Epub 2021 Nov 20.

Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science and Engineering, Hubei University, Wuhan 430062, China.

SnO films as a promising electron transport layer (ETL) have been widely used in planar-type perovskite solar cells to achieve an impressive improvement in the conversion efficiency. However, compared with a mesoporous ETL, the interfacial charge carrier transfer of the SnO ETL is severely limited due to the issues of oxygen vacancy defects and crystal lattice mismatch between SnO and the perovskite, which generally leads to the growth of randomly stacked and porous perovskite layers and subsequently impacts the charge transport and transfer properties. In this work, we developed a facile approach by inducing a bifunctional molecule, β-alanine, into the SnO ETL, which can serve as a bridge to modulate the interfacial charge transfer and the perovskite crystallization kinetics. Benefited by the interfacial β-alanine, we grew a highly orientational perovskite layer that exhibited superior charge transport properties. Meanwhile, the β-alanine caused an intimate connection between the perovskite and SnO to enhance the interfacial charge transfer. As a result, the power conversion efficiency (PCE) of the β-alanine-modified device achieved a much-improved value of 19.67% and showed high reproducibility. This work provides a way for developing a high-performance ETL toward the scalable fabrication of highly efficient PSCs.
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http://dx.doi.org/10.1021/acsami.1c17260DOI Listing
November 2021

Efficacy of Aumolertinib (HS-10296) in Patients with Advanced EGFR T790M+ NSCLC: Updated Post NMPA-approval Results from the APOLLO Registrational Trial.

J Thorac Oncol 2021 11 18. Epub 2021 Nov 18.

Department of Oncology, Binzhou Medical University Hospital, Binzhou, China.

Introduction: Aumolertinib (formerly almonertinib; HS-10296) is a novel third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) with demonstrated activity against EGFR sensitizing mutations and EGFR T790M mutation.

Methods: Patients with locally advanced or Metastatic non-small cell lung cancer (NSCLC) who developed an EGFR T790M mutation after progression on first- or second-generation EGFR TKI therapy were enrolled into this registrational phase 2 trial of aumolertinib at 110 mg orally once daily (NCT02981108). The primary endpoint was objective response rate (ORR) by independent central review (ICR).

Results: A total of 244 patients with EGFR T790M-positive NSCLC were enrolled. The ORR by ICR was 68.9% (95% CI: 62.6-74.6). The disease control rate (DCR) was 93.4% (95% CI: 89.6-96.2). The median duration of response (DOR) was 15.1 months (95% CI: 12.5-16.6). The median progression-free survival was12.4 months (95% CI: 9.7-15.0). Among 23 patients with evaluable central nervous system (CNS) metastases, the CNS-ORR and CNS-DCR were 60.9% (95% CI: 38.5-80.3) and 91.3% (95% CI: 72.0-98.9), respectively. The median CNS-DOR was 12.5 months (95% CI: 5.6-NR). Treatment-related adverse events of ≥ grade 3 occurred in 16.4% of patients, with the most common being blood creatine phosphokinase increased (7%) and alanine aminotransferase increased (1.2%). The relative dose density of aumolertinib was 99.2% in this study.

Conclusion: Aumolertinib is an effective and well-tolerated third-generation EGFR TKI for patients with EGFR T790M-positive advanced NSCLC after disease progression on first- and second-generation EGFR TKI therapy. Based on these findings, aumolertinib was approved in China for EGFR T790M-positive patients.
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http://dx.doi.org/10.1016/j.jtho.2021.10.024DOI Listing
November 2021

Gender differences in the associations between tobacco smoke exposure and depressive symptoms among U.S. adults: NHANES 2007-2018.

J Psychiatr Res 2021 Nov 9. Epub 2021 Nov 9.

Animal Laboratory Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address:

Background: Findings concerning gender differences in the associations between tobacco smoke exposure (TSE) and depression are inconsistent. This study aimed to investigate the gender-specific associations between active and passive TSE with depressive symptoms in a large, nationally representative sample of U.S. adults.

Methods: Data were from 27,175 adults aged ≥20 years in the 2007-2018 National Health and Nutrition Examination Survey (NHANES). Depressive symptoms were assessed using the Patient Health Questionnaire-9 (PHQ-9). Multivariable logistic regression was used to adjust for possible confounders. Whether the TSE-depression relationships may differ by age, race/ethnicity, socioeconomic status, body mass index (BMI), and self-reported health status was examined.

Results: After adjustment for lifestyle- and health-related variables, no significant associations between active (OR, 1.16 [95% CI, 0.87-1.55]) and passive TSE (OR, 0.84 [95% CI, 0.59-1.19]) and depressive symptoms were found among men. Among women, active TSE was associated with depressive symptoms (OR, 1.90 [95% CI, 1.51-2.39]), while the association for passive TSE was nonsignificant (OR, 1.11 [95% CI, 0.91-1.34]) after adjusting for lifestyle- and health-related variables. Interaction and subgroup analyses showed that self-reported health status could modify the relationship between passive TSE and depressive symptoms among women. Furthermore, a dose-response relationship between serum cotinine and depressive symptoms was found in women, but not in men.

Conclusions: This study suggests a stronger TSE-depression association in women than in men. Understanding these gender-specific patterns and identifying the potential moderators of such relationships will enable better targeting of public health interventions.
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http://dx.doi.org/10.1016/j.jpsychires.2021.11.013DOI Listing
November 2021

Representation Learning for Integrating Multi-domain Outcomes to Optimize Individualized Treatments.

Adv Neural Inf Process Syst 2020 Dec;33:17976-17986

Department of Biostatistics, Columbia University, New York, NY 10032.

For mental disorders, patients' underlying mental states are non-observed latent constructs which have to be inferred from observed multi-domain measurements such as diagnostic symptoms and patient functioning scores. Additionally, substantial heterogeneity in the disease diagnosis between patients needs to be addressed for optimizing individualized treatment policy in order to achieve precision medicine. To address these challenges, we propose an integrated learning framework that can simultaneously learn patients' underlying mental states and recommend optimal treatments for each individual. This learning framework is based on the measurement theory in psychiatry for modeling multiple disease diagnostic measures as arising from the underlying causes (true mental states). It allows incorporation of the multivariate pre- and post-treatment outcomes as well as biological measures while preserving the invariant structure for representing patients' latent mental states. A multi-layer neural network is used to allow complex treatment effect heterogeneity. Optimal treatment policy can be inferred for future patients by comparing their potential mental states under different treatments given the observed multi-domain pre-treatment measurements. Experiments on simulated data and a real-world clinical trial data show that the learned treatment polices compare favorably to alternative methods on heterogeneous treatment effects, and have broad utilities which lead to better patient outcomes on multiple domains.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593913PMC
December 2020

X-ray repair cross-complementing protein 1 (XRCC1) loss promotes β-lapachone -induced apoptosis in pancreatic cancer cells.

BMC Cancer 2021 Nov 17;21(1):1234. Epub 2021 Nov 17.

Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University/School of Basic Medical Science, Fujian Medical University, Fuzhou City, 350122, Fujian Province, China.

Background: β-lapachone (β-lap), the NQO1 bioactivatable drug, is thought to be a promising anticancer agent. However, the toxic side effects of β-lap limit the drug use, highlighting the need for a thorough understanding of β-lap's mechanism of action. β-lap undergoes NQO1-dependent futile redox cycling, generating massive ROS and oxidative DNA lesions, leading to cell death. Thus, base excision repair (BER) pathway is an important resistance factor. XRCC1, a scaffolding component, plays a critical role in BER.

Methods: We knocked down XRCC1 expression by using pLVX-shXRCC1 in the MiaPaCa2 cells and BxPC3 cells and evaluated β-lap-induced DNA lesions by γH2AX foci formation and alkaline comet assay. The cell death induced by XRCC1 knockdown + β-lap treatment was analysed by relative survival, flow cytometry and Western blotting analysis.

Results: We found that knockdown of XRCC1 significantly increased β-lap-induced DNA double-strand breaks, comet tail lengths and cell death in PDA cells. Furthermore, we observed combining XRCC1 knockdown with β-lap treatment switched programmed necrosis with β-lap monotherapy to caspase-dependent apoptosis.

Conclusions: These results indicate that XRCC1 is involved in the repair of β-lap-induced DNA damage, and XRCC1 loss amplifies sensitivity to β-lap, suggesting targeting key components in BER pathways may have the potential to expand use and efficacy of β-lap for gene-based therapy.
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http://dx.doi.org/10.1186/s12885-021-08979-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600733PMC
November 2021

Development of a nomogram for predicting the risk of left ventricular diastolic function in subjects with type-2 diabetes mellitus.

Int J Cardiovasc Imaging 2021 Nov 16. Epub 2021 Nov 16.

Department of Endocrinology, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China.

Left ventricular diastolic dysfunction (LVDD) can be affected by many factors, including epicardial adipose tissue (EAT), obesity and type-2 diabetes mellitus (T2DM). The aim of this study was to establish and validate an easy-to-use nomogram that predicts the severity of LVDD in patients with T2DM. This is a retrospective study of 84 consecutive subjects with T2DM admitted to the Endocrinology Department, the First People's Hospital of Zunyi City between January 2015 and October 2020. Several echocardiographic characteristics were used to diagnose diastolic dysfunction according to the 2016 diastolic dysfunction ASE guidelines. Anthropometric, demographic, and biochemical parameters were collected. Through a least absolute shrinkage and selection operator (LASSO) regression model, we reduced the dimensionality of the data and determined factors for the nomogram. The mean follow-up was 25.97 months. Cases were divided into two groups, those with LVDD (31) and those without (53). LASSO regression identified total cholesterol (Tol.chol), low-density lipoprotein (LDL), right ventricular anterior wall (RVAW) and epicardial adipose tissue (EAT) were identified as predictive factors in the nomogram. The ROC curve analysis demonstrated that the AUC value for most clinical paramerters was higher than 0.6. The nomogram can be used to promote the individualized prediction of LVDD risk in T2DM patients, and help to prioritize patients diagnosed with echocardiography.
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http://dx.doi.org/10.1007/s10554-021-02338-5DOI Listing
November 2021

Nomogram Based on Log Odds of Positive Lymph Nodes Predicting Cancer-Specific Survival in Patients With T3 and T4 Gallbladder Cancer After Radical Resection.

Front Surg 2021 27;8:675661. Epub 2021 Oct 27.

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

The aim of this study based on log odds of positive lymph nodes (LODDS) is to develop and validate an effective prognostic nomogram for patients with T3 and T4 gallbladder cancer (GBC) after resection. A total of 728 T3 and T4 gallbladder cancer patients after resection from the Surveillance, Epidemiology, and End Results (SEER) database, randomly divided into training cohort and validation cohort according to 7:3. Another 128 patients from The Second Affiliated Hospital of Nanchang University for external validation. The nomograms were built by the Cox regression model and the Fine and Grey's model. Concordance index (C-index), calibration curve and the area under receiver operating characteristic (ROC) curve (AUC) were used to evaluate the nomogram and internal verification. The decision curve analysis (DCA) was used to measure clinical applicability. LODDS was independent prognostic predictor for overall survival (OS) and cancer-specific survival (CSS), and established the nomograms on this basis. The nomogram we have established has a good evaluation effect, with a C-index of 0.719 (95%CI, 0.707-0.731) for OS and 0.747 (95%CI, 0.733-0.760) for CSS. The calibration curves of OS and CSS both showed good calibration capability, and the AUC for predicting 1-, 2-, and 3-year 0.858, 0.848 were and 0.811 for OS, and 0.794, 0.793, and 0.750 for CSS. The DCA of nomograms both showed good clinical applicability. The nomogram can provide effective OS and CSS prediction for patients with advanced gallbladder cancer after surgery.
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http://dx.doi.org/10.3389/fsurg.2021.675661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578716PMC
October 2021

Learning Individualized Treatment Rules for Multiple-Domain Latent Outcomes.

J Am Stat Assoc 2021 19;116(533):269-282. Epub 2020 Oct 19.

Department of Biostatistics, Mailman School of Public Health, Columbia University, New York, NY 10032.

For many mental disorders, latent mental status from multiple-domain psychological or clinical symptoms may perform as a better characterization of the underlying disorder status than a simple summary score of the symptoms, and they may also serve as more reliable and representative features to differentiate treatment responses. Therefore, in order to address the complexity and heterogeneity of treatment responses for mental disorders, we provide a new paradigm for learning optimal individualized treatment rules (ITRs) by modeling patients' latent mental status. We first learn the multi-domain latent states at baseline from the observed symptoms under a restricted Boltzmann machine (RBM) model, through which patients' heterogeneous symptoms are represented using an economical number of latent variables and yet remains flexible. We then optimize a value function defined by the latent states after treatment by exploiting a transformation of the observed symptoms based on the RBM without modeling the relationship between the latent mental states before and after treatment. The optimal treatment rules are derived using a weighted large margin classifier. We derive the convergence rate of the proposed estimator under the latent models. Simulation studies are conducted to test the performance of the proposed method. Finally, we apply the developed method to real world studies and we demonstrate the utility and advantage of our method in tailoring treatments for patients with major depression, and identify patient subgroups informative for treatment recommendations.
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http://dx.doi.org/10.1080/01621459.2020.1817751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589272PMC
October 2020

Mechanism of transepithelial migration of lymphocytes into the milk in porcine mammary glands.

J Reprod Immunol 2021 Oct 29;149:103440. Epub 2021 Oct 29.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, PR China. Electronic address:

Lymphocytes in the colostrum play many important roles during lactation, including protecting newborn piglets against infections. The lymphocytes constantly enter the mammary gland from the mother's bloodstream before and during lactation. However, little is known about the mechanism of transport of maternal lymphocytes across the mammary glands into the milk (lumen). In this study, the maternal lymphocytes were detected in sow colostrum by immunofluorescent staining and fluorescence-activated cell sorting and lymphocytes were observed transmigrating into the breast acinar lumen. Furthermore, immunohistochemical staining revealed that CD3 T, γδ T, and IgA B cells were primarily located at the base area of the mammary gland. Meanwhile, more lactating alveoli and blood capillaries were distributed in this area. Finally, a mammary epithelial cell (EpH4-Ev)/T cell co-culture system was established to explore the mechanism of lymphocyte transmigration across the mammary epithelial cells. The expression of CCL2 and CCL28 in EpH4-Ev cells, which facilitated the transmigration of lymphocytes, significantly increased in the presence of prolactin. Our results provide a better understanding of the concept of lactogenic immunity and pave the way for vaccination strategies for the induction of lactogenic immunity in pregnant swine.
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http://dx.doi.org/10.1016/j.jri.2021.103440DOI Listing
October 2021

Anti-Inflammatory Activity of Three Triterpene from L. in Lipopolysaccharide-Stimulated RAW264.7 Cells.

Int J Mol Sci 2021 Nov 5;22(21). Epub 2021 Nov 5.

Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, China.

Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effects of the triterpenes according to their structural differences. MTT, Griess, and immunofluorescence assays, ELISA, flow cytometry, and Western blotting, were performed. The release of LPS-induced pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin (IL-6), was significantly inhibited by OA, AA, and MA at the same concentration, and AA and MA promoted the production of anti-inflammatory factor IL-10. OA, AA, and MA inhibited LPS-induced NF-κB nuclear translocation in RAW264.7 cells. OA and AA inhibited the phosphorylation of ERK1/2, P38, and JNK1/2 in LPS-stimulated RAW264.7 cells. Moreover, OA increased LPS-induced Nrf2 expression and decreased Keap1 expression in RAW264.7 cells. OA, AA, and MA inhibited LPS-stimulated intracellular reactive oxygen species (ROS) production and alleviated mitochondrial membrane potential depletion. Overall, our data suggested that OA, AA, and MA exhibited significant anti-inflammatory effects in vitro. In particular, OA and AA take effects through the MAPKs, NF-κB, and Nrf2 signaling pathways.
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http://dx.doi.org/10.3390/ijms222112009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584548PMC
November 2021

Fibulin 2 Is Hypermethylated and Suppresses Tumor Cell Proliferation through Inhibition of Cell Adhesion and Extracellular Matrix Genes in Non-Small Cell Lung Cancer.

Int J Mol Sci 2021 Oct 31;22(21). Epub 2021 Oct 31.

Section Pathology of the Institute of Forensic Medicine, Jena University Hospital, Friedrich Schiller University Jena, Am Klinikum 1, 07747 Jena, Germany.

Fibulins (FBLNs), interacting with cell adhesion receptors and extracellular matrix (ECM) components, play multiple roles in ECM structures and tissue functions. Abnormal expression of FBLN2, one of the fibulin family members, contributes to tumor initiation and development. However, the function of FBLN2 in human non-small cell lung cancer (NSCLC) has not yet been elucidated. In this study, we found that FBLN2 was downregulated in 9 out of 11 lung cancer cell lines compared to normal bronchial epithelial cells, which was associated with DNA hypermethylation. Primary lung squamous cell carcinoma expressed significantly more FBLN2 protein compared to adenocarcinoma ( = 0.047). Ectopic expression of FBLN2 led to decreased cell proliferation, migration and invasion, accompanied by inactivated MAPK/ERK and AKT/mTOR pathways, while FBLN2 siRNA knockdown resulted in an opposite biological behaviour in NSCLC cells. Additionally, overexpression of FBLN2 led to dysregulation of cell adhesion molecules, ECM markers and a panel of lysate/exosome-derived-microRNAs, which are involved in cell adhesion and ECM remodelling. Taken together, our data indicate that FBLN2 is methylated and exerts a tumor suppressor function through modulation of MAPK/ERK and AKT pathways and regulation of cell adhesion and ECM genes. Moreover, FBLN2 might be a potential biomarker for the sub-classification of NSCLC.
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http://dx.doi.org/10.3390/ijms222111834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584407PMC
October 2021

VDAC1 Negatively Regulates Floral Transition in .

Int J Mol Sci 2021 Oct 27;22(21). Epub 2021 Oct 27.

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

Voltage-dependent anion channels (VDACs) are the most important proteins in mitochondria. They localize to the outer mitochondrial membrane and contribute to the metabolite transport between the mitochondria and cytoplasm, which aids plant growth regulation. Here, we report that VDAC1 is involved in the floral transition, with the loss of function, resulting in an early-flowering phenotype. is expressed ubiquitously in . To identify the flowering pathway integrators that may be responsible for AtVDAC1's function during the floral transition, an RNA-seq analysis was performed. In total, 106 differentially expressed genes (DEGs) were identified between wild-type and mutant seedlings. However, none were involved in flowering-related pathways. In contrast, AtVDAC1 physically associated with FLOWERING LOCUS T. Thus, in the floral transition, AtVDAC1 may function partly through the FLOWERING LOCUS T protein.
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http://dx.doi.org/10.3390/ijms222111603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584032PMC
October 2021

Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma.

Am J Cancer Res 2021 15;11(10):4746-4767. Epub 2021 Oct 15.

Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA.

Ubiquitin specific peptidase-2 (USP2) plays important roles in a myriad of cellular activities through deubiquitinating target proteins and its implications in various diseases, especially cancers, are starting to emerge. Our current understanding on USP2 expression in subjects with hepatocellular carcinoma (HCC) and its roles in the pathogenesis of HCC is limited. In this study, we found that USP2 protein and mRNA levels were significantly dysregulated in HCC tumor (HCC-T) when compared to adjacent non-tumor (HCC-NT) or normal liver tissues from both human and mouse HCC model. Among the USP2 isoforms, USP2b was the predominant isoform in the normal liver and markedly down-regulated in HCC-T tissues in both human and mice. Data from overexpression, chemical inhibition and knockout studies consistently demonstrated that USP2b promoted cell proliferation, colony formation and wound healing in HepG2 and Huh 7 cells. On the other hand, USP2b exhibited proapoptotic and pronecrtotic activities through enhancing bile acid-induced apoptosis and necrosis in both HepG2 and Huh 7 cells. Unbiased proteomic analysis of USP2-knockout (KO) and parental HepG2 cells resulted in identification of USP2-regulated downstream target proteins involved in cell proliferation, apoptosis, and tumorigenesis, including serine/threonine kinase 4 (STK4), epidermal growth factor receptor (EGFR), dipeptidyl peptidase 4 (DPP4) and fatty acid binding protein 1 (FABP1). In conclusion, USP2b expression was dysregulated in subjects with HCC and contributed to the pathogenesis of HCC by promoting cell proliferation and exerting proapoptotic and pronecrotic activities. The findings provide the molecular basis for developing therapies for HCC through modulating USP2b expression or activities.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569343PMC
October 2021

S100A9 Activates the Immunosuppressive Switch Through the PI3K/Akt Pathway to Maintain the Immune Suppression Function of Testicular Macrophages.

Front Immunol 2021 26;12:743354. Epub 2021 Oct 26.

Institute of Reproductive Health, Tongji Medical College, Hua Zhong University of Science and Technology, Wuhan, China.

Macrophages are functionally plastic and can thus play different roles in various microenvironments. Testis is an immune privileged organ, and testicular macrophages (TMs) show special immunosuppressive phenotype and low response to various inflammatory stimuli. However, the underlying mechanism to maintain the immunosuppressive function of TMs remains unclear. S100A9, a small molecular Ca binding protein, is associated with the immunosuppressive function of macrophages. However, no related research is available about S100A9 in mouse testis. In the present study, we explored the role of S100A9 in TMs. We found that S100A9 was expressed in TMs from postnatal to adulthood and contributed to maintaining the immunosuppressive phenotype of TMs, which is associated with the activation of PI3K/Akt pathway. S100A9 treatment promotes the polarization of bone marrow-derived macrophages from M0 to M2 . S100A9 was significantly increased in TMs following UPEC-infection and elevated S100A9 contributed to maintain the M2 polarization of TMs. Treatment with S100A9 and PI3K inhibitor decreased the proportion of M2-type TMs in control and UPEC-infected mouse. Our findings reveal a crucial role of S100A9 in maintaining the immunosuppressive function of TMs through the activation of PI3K/Akt pathway, and provide a reference for further understanding the mechanism of immunosuppressive function of TMs.
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http://dx.doi.org/10.3389/fimmu.2021.743354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576360PMC
October 2021

Ethylene response factor AcERF91 affects ascorbate metabolism via regulation of GDP-galactose phosphorylase encoding gene (AcGGP3) in kiwifruit.

Plant Sci 2021 Dec 24;313:111063. Epub 2021 Sep 24.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China. Electronic address:

Kiwifruit is known as 'the king of vitamin C' because of the high content of ascorbic acid (AsA) in the fruit. Deciphering the regulatory network and identification of the key regulators mediating AsA biosynthesis is vital for fruit nutrition and quality improvement. To date, however, the key transcription factors regulating AsA metabolism during kiwifruit developmental and ripening processes remains largely unknown. Here, we generated a putative transcriptional regulatory network mediating ascorbate metabolism by transcriptome co-expression analysis. Further studies identified an ethylene response factor AcERF91 from this regulatory network, which is highly co-expressed with a GDP-galactose phosphorylase encoding gene (AcGGP3) during fruit developmental and ripening processes. Through dual-luciferase reporter and yeast one-hybrid assays, it was shown that AcERF91 is able to bind and directly activate the activity of the AcGGP3 promoter. Furthermore, transient expression of AcERF91 in kiwifruit fruits resulted in a significant increase in AsA content and AcGGP3 transcript level, indicating a positive role of AcERF91 in controlling AsA accumulation via regulation of the expression of AcGGP3. Overall, our results provide a new insight into the regulation of AsA metabolism in kiwifruit.
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http://dx.doi.org/10.1016/j.plantsci.2021.111063DOI Listing
December 2021

Weight Change, Lifestyle and Mortality in Patients with Type 2 Diabetes.

J Clin Endocrinol Metab 2021 Nov 6. Epub 2021 Nov 6.

Department of Nutrition, Harvard TH Chan School of Public Health, 665 Huntington Avenue, Boston, MA, USA.

Objective: To examine the association between weight change and mortality among participants with incident type 2 diabetes (T2D) and evaluate the impact of adopting a healthy lifestyle on this association.

Research Design And Methods: This prospective analysis included 11,262 incident T2D patients from the Nurses' Health Study and Health Professionals Follow-up Study. We assessed weight change bracketing T2D diagnosis in relation to mortality. We also examined potential effect modification by a healthy lifestyle consisting of high-quality diet, regular physical activity, non-smoking status and moderate alcohol consumption.

Results: On average, T2D patients lost 2.3 kg during a two-year time-window spanning the T2D diagnosis, and body weight increased afterwards following a trajectory similar to that of non-diabetics. Compared with patients with a stable weight, T2D patients who lost ≥10% body weight had a 21% (95% CI: 9%, 35%) increased all-cause mortality. Lifestyle significantly modified these associations: the hazard ratios (95% CIs) of all-cause mortality comparing ≥10% weight loss with stable weight were 1.63 (1.26, 2.09) among participants with a deteriorated lifestyle, 1.27 (1.11, 1.46) for a stable lifestyle, and 1.02 (0.81, 1.27) for an improved lifestyle (Pinteraction <0.001). Major weight loss was associated with increased cause-specific mortality and similar effect modifications by lifestyle were also observed.

Conclusions: Significant weight loss upon T2D incidence was associated with increased mortality, although improved lifestyle quality abolished these associations. These results highlight the role of adopting a healthy lifestyle for newly diagnosed T2D patients, especially among those who might lose weight unintentionally, and improving long-term survival.
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http://dx.doi.org/10.1210/clinem/dgab800DOI Listing
November 2021

Identification of phytochemicals and antioxidant activity of Premna microphylla Turcz. stem through UPLC-LTQ-Orbitrap-MS.

Food Chem 2021 Oct 27;373(Pt B):131482. Epub 2021 Oct 27.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address:

Premna microphylla Turcz. is a commonly used traditional Chinese medicine totreatdysentery and appendicitis. Present study is focused to explore antioxidants and other compounds in the Premna microphylla Turcz. stem. Assessment of chemical composition was done with high sensitivity UPLC-LTQ-Orbitrap-MS and for Separation Thermo Hypersil Gold (100 mm × 2.1 mm, 1.9 µm) was used while electrospray ionization (ESI) was used for the mass spectrometry. 18 compounds were identified including Vitexin (1), Schaftoside (2), Vicenin-2 (3), Apigenin-6, 8-di-C-arabinoside (4), Apigenin-7-O-β-d-glucoside (5), Carnosic acid (6), Apigenin-8-C-β-d-xylopyranoside (7), Prostratin (8), Aurantio-obtusin-β-d-glucoside (9), Royleanone (10), 5-hydroxy-7,3',4'-Trimethoxy flavonols (11), 6-Hydroxy-5,6-dehydrosugiol (12), 14-deoxycoleon (13), Arucadiol (14), Obtusinone-B (15), Trehalose (16), Citric acid (17) and Betaine (18). Among these, 6 compounds including (6), (8), (9), (16), (17) and (18) were identified first time within this genus and plant. Study highlights the importance of Premna microphylla Turcz. stem extract for strong therapeutic potential against oxidation-related diseases.
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http://dx.doi.org/10.1016/j.foodchem.2021.131482DOI Listing
October 2021

Two-Dimensional Fluorinated Covalent Organic Frameworks with Tunable Hydrophobicity for Ultrafast Oil-Water Separation.

Angew Chem Int Ed Engl 2021 Oct 28. Epub 2021 Oct 28.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 200240, China), .

Covalent organic frameworks (COFs) hold great potentials for addressing the challenge of highly efficient oil-water separation, but they are restricted by the poor wettability and processability as crystalline membranes. Here we report the design and synthesis of two-dimensional (2D) robust COFs with controllable hydrophobicity and processability, allowing the COF layers to be directly grown on the support surfaces. Three 2D COFs with AA or ABC stacking are prepared by condensation of triamines with fluorine and/or isopropyl groups and perfluorodialdehyde. They all show excellent tolerance to water, acid, and base, with water contact angles (CA) of 111.5-145.8°. The two COFs with isopropyl and fluorine mixtures can grow as a coating on a stainless-steel net (SSN) substrate, whereas the one with only fluorine substituents cannot. The superhydrophobic [email protected] coating with water CA of up to 150.1° displays high water-resistance and self-cleaning properties, enabling high oil-water separation performances with an efficiency of over 99.5 % and a permeation flux of 2.84×10  L m  h , which are among the highest values reported for state-of-the-art membranes.
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http://dx.doi.org/10.1002/anie.202113348DOI Listing
October 2021

Angiopoietin-like protein 2 deficiency promotes periodontal inflammation and alveolar bone loss.

J Periodontol 2021 Oct 28. Epub 2021 Oct 28.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Background: Human periodontitis is a highly prevalent inflammatory disease that leads to connective tissue degradation, alveolar bone resorption, and tooth loss. Angiopoietin-like 2 (ANGPTL2) regulates chronic inflammation in various diseases and is functionally involved in maintaining tissue homeostasis and promoting tissue regeneration, but there is limited information about its function in periodontitis. Here we investigated the expression and explicit role of ANGPTL2 in periodontitis.

Methods: Immunohistochemistry and quantitative real-time PCR (qRT-PCR) were used to detect the ANGPTL2 expression in periodontal tissues and periodontal ligament cells (PDLCs). A ligature-induced periodontitis model was generated in wild-type and ANGPTL2 knockout mice. qRT-PCR and enzyme-linked immunosorbent assay were used to assess the production of inflammatory cytokines and matrix metalloproteinases (MMPs) in cultured PDLCs. Western blot was performed to detect proteins in relevant signaling pathways.

Results: Increased ANGPTL2 expression was observed in inflamed periodontal tissues and PDLCs. ANGPTL2 deficiency promoted alveolar bone loss with enhanced osteoclastogenesis and inflammatory reactions in ligature-induced periodontitis. Downregulation of ANGPTL2 remarkably enhanced expression levels of interleukin (IL)-6, IL-8, MMP1, and MMP13 in Porphyromonas gingivalis lipopolysaccharide-induced PDLCs, whereas ANGPTL2-overexpressing PDLCs showed opposite trends. ANGPTL2 downregulation activated STAT3 and nuclear factor-κB pathways and blocked Akt signaling under inflammatory environment. Treatment with a STAT3 inhibitor partially suppressed the inflammatory reaction of PDLCs mediated by ANGPTL2 knockdown.

Conclusion: Our study provides the first evidence of an anti-inflammatory effect of ANGPTL2 in murine periodontitis. The findings demonstrate the critical and protective role of ANGPTL2 in alveolar bone loss and periodontal inflammation. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/JPER.21-0290DOI Listing
October 2021
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