Publications by authors named "Chen Ling"

2,576 Publications

  • Page 1 of 1

Comprehensive analysis of NGS and ARMS-PCR for detecting EGFR mutations based on 4467 cases of NSCLC patients.

J Cancer Res Clin Oncol 2021 Oct 24. Epub 2021 Oct 24.

Clinical Molecular Medicine Testing Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Background: By comparing the detection rate and type of targeted gene mutations in non-small cell lung cancer (NSCLC) between amplification refractory mutation system PCR (ARMS-PCR) and next-generation sequencing (NGS), the characteristics and application advantages of non-small cell lung cancer detection are explained, providing a basis for clinicians to effectively select the corresponding detection methods.

Methods And Materials: The cases of targeted genes for lung cancer were selected from the First Affiliated Hospital of Chongqing Medical University from January 2016 to October 2020. A sample of 4467 cases was selected, and they were diagnosed with NSCLC by Pathological biopsy. Sample sources include surgical resection, bronchoscope biopsy, metastatic biopsy, blood, sputum, cytology of pleural effusion. Among them, 3665 cases were detected by ARMS-PCR technique, and 802 cases were detected by NGS technology. The detection rate and type of ARMS-PCR and NGS techniques for EGFR gene mutations (including exon 18, exon 19, exon 20, exon 21 and so on) in different NSCLC samples were compared, respectively.

Results: The total mutation rate of EGFR gene detected by ARMS-PCR was 47.6% while 42.4% detected by NGS which indicated that there was a significant difference between the two methods in detecting total mutation of EGFR gene (P < 0.001). In different exons, the EGFR mutation rate detected by two methods is various. The mutation rate of exon 19 by ARMS-PCR detection was evidently higher than that of NGS detection, while the mutation rate of exons 20 and 21 by ARMS-PCR detection were statistically significantly lower than that of NGS detection. Moreover, the multiple mutation rate detected by NGS was 16.3% which was much higher than the 2.7% detected by ARMS-PCR with statistically different.

Conclusion: It showed that NGS could direct the drug use for the resistant patients. However, some rare loci could be detected by NGS but the importance and directed meaning are still unknown and the number of rare mutations is rare too. Further research on new biomarkers and technique is still needed for early diagnosis, directing drug use and assessing the therapy prognosis.
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http://dx.doi.org/10.1007/s00432-021-03818-wDOI Listing
October 2021

B Lymphocyte Subsets in Children With Steroid-Sensitive Nephrotic Syndrome: A Longitudinal Study.

Front Pediatr 2021 8;9:736341. Epub 2021 Oct 8.

Department of Nephrology, Beijing Children's Hospital, Capital Medical University, Beijing, China.

B-cell subsets may be involved in the pathogenesis of childhood steroid-sensitive nephrotic syndrome (SSNS). Horizontal control studies have shown that homeostasis of B-cell subsets changes at different stages of the SSNS. However, there is a lack of longitudinal studies that have investigated dynamic changes in B cell subpopulations. Blood samples were collected at the following time points from 15 children with SSNS treated at our hospital: before administration of steroid and after 3 days, 1 week, and 3, 6, 9, and 12 months. The proportions of circulating total B cells (CD19), transitional B cells (CD19CD24CD38), mature B cells (CD19CD24CD38), and memory B cells (CD19CD24CD38) were monitored by flow cytometry. The proportion of CD19+ B cells before steroid administration was significantly higher than that observed at any other time point or in the healthy control (HC) group ( < 0.001). However, this proportion was significantly lower than that in the HC group at 12 months ( = 0.031). Transitional B cells before (%BL 9.5 ± 4.4) and 3 days after steroid administration (%BL 10.6 ± 5.1) were significantly higher than at any other time point or in the HC group ( < 0.001). Although these cells declined after the 3rd day the percentage was still significantly lower than that of the HC group at 12 months ( = 0.029). Memory B cells increased gradually after steroid administration and decreased to the normal range after 9 months. B cell subpopulations show dynamic changes in children with SSNS, suggesting that they are involved in the pathogenesis of the disorder. Further studies are required to determine whether this change can guide individualized treatment.
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http://dx.doi.org/10.3389/fped.2021.736341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532540PMC
October 2021

Habitat Imaging-Based F-FDG PET/CT Radiomics for the Preoperative Discrimination of Non-small Cell Lung Cancer and Benign Inflammatory Diseases.

Front Oncol 2021 6;11:759897. Epub 2021 Oct 6.

Research Center for Healthcare Data Science, Zhejiang Lab, Hangzhou, China.

Purpose: To propose and evaluate habitat imaging-based F-fluorodeoxyglucose (F-FDG) positron emission tomography/computed tomography (PET/CT) radiomics for preoperatively discriminating non-small cell lung cancer (NSCLC) and benign inflammatory diseases (BIDs).

Methods: Three hundred seventeen F-FDG PET/CT scans were acquired from patients who underwent aspiration biopsy or surgical resection. All volumes of interest (VOIs) were semiautomatically segmented. Each VOI was separated into variant subregions, namely, habitat imaging, based on our adapted clustering-based habitat generation method. Radiomics features were extracted from these subregions. Three feature selection methods and six classifiers were applied to construct the habitat imaging-based radiomics models for fivefold cross-validation. The radiomics models whose features extracted by conventional habitat-based methods and nonhabitat method were also constructed. For comparison, the performances were evaluated in the validation set in terms of the area under the receiver operating characteristic curve (AUC). Pairwise t-test was applied to test the significant improvement between the adapted habitat-based method and the conventional methods.

Results: A total of 1,858 radiomics features were extracted. After feature selection, habitat imaging-based F-FDG PET/CT radiomics models were constructed. The AUC of the adapted clustering-based habitat radiomics was 0.7270 ± 0.0147, which showed significantly improved discrimination performance compared to the conventional methods (p <.001). Furthermore, the combination of features extracted by our adaptive habitat imaging-based method and non-habitat method showed the best performance than the other combinations.

Conclusion: Habitat imaging-based F-FDG PET/CT radiomics shows potential as a biomarker for discriminating NSCLC and BIDs, which indicates that the microenvironmental variations in NSCLC and BID can be captured by PET/CT.
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http://dx.doi.org/10.3389/fonc.2021.759897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526895PMC
October 2021

Overexpressed miR-375-Loaded Restrains Development of Cervical Cancer Through Down-Regulation of Frizzled Class Receptor 4 (FZD4) with Liposome Nanoparticle as a Carrier.

J Biomed Nanotechnol 2021 Sep;17(9):1882-1889

The First Department of Gynecology of Shanxi Provincial People's Hospital, Taiyuan, 030012, Shanxi, China.

Dysregulation expression of miR-375 is noted to correlate with progression of cervical cancer. This study attempted to investigate the impact of overexpressed miR-375-loaded liposome nanoparticles on proliferation of cervical cancer (CC), to provide an insight on pathogenesis of CC disorder. CC cells were co-cultured with pure liposome nanoparticles (empty vector group), miR-375 agonist-loaded liposome nanoparticles, or transfected with miR-375 antagonist. Besides, some cells were exposed to TGF-/Smads signaling pathway inhibitor or activator whilst cell proliferation was assessed by MTT assay, and expressions of FZD4 and miR-375 were determined. Western blot analysis was carried out to detect the expression of TGF- pathway factors (TGF-, Smad2, Smad7, p-Smad2) and its downstream Smads pathway. The interaction between miR-375 and FZD4 was evaluated by dual-luciferase reporter gene assay. Overexpression of miR-375 induced arrest at the G0/G1 phase of cell cycle and elevation of Smad2 protein expression ( <0.05), with lower expressions of TGF-, Smad7, p-Smad2, and FZD4, while transfection with miR-375 inhibitor exhibited opposite activity. Presence of miR-375 agonist-loaded liposome nanoparticles induced decreased cell proliferation. There was a targeting relationship between miR-375 and FZD4, and administration with TGF-/Smads agonist resulted in increased miR-375 and Smad2 expressions, as well as decreased TGF-, Smad7, p-Smad2, FZD4 protein expression, and the number of S phase and G2/M phase cells ( < 0.05). The signaling inhibitor oppositely suppressed cell proliferation decreasing miR-375 expression. miR-375-loaded liposome nanoparticles activated TGF-/Smads signaling pathway to restrain cell cycle and suppress cell division, and proliferation through targeting FZD4 in CC. Its molecular mechanism is related to activation of TGF-/Smads signaling pathway.
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http://dx.doi.org/10.1166/jbn.2021.3145DOI Listing
September 2021

A pyroptosis-related lncRNA signature predicts prognosis and immune microenvironment in head and neck squamous cell carcinoma.

Int Immunopharmacol 2021 Oct 20;101(Pt B):108268. Epub 2021 Oct 20.

Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Background: Pyroptosis, a type of regulated cell death controlled by the gasdermin family of proteins to form plasma membrane pores is known. Nonetheless, the function of pyroptosis-related long non-coding RNAs (lncRNAs) in this process still lacks exhaustive elucidation in head and neck squamous cell carcinoma (HNSCC). Other attributes encompassing the function of such lncRNAs in the immune microenvironment and potential prognosis for HNSCC are yet to see the light of the day. This work was designed to probe the possible prognostic worth of pyroptosis-related lncRNAs along with their impact on the immune microenvironment in the case of HNSCC.

Methods: The Cancer Genome Atlas (TCGA) database was the source of RNA-sequencing data of HNSCC patients. Pearson correlation analysis was employed to scour for lncRNAs linked to pyroptosis based on 40 genes related to the process. Following the construction of a pyroptosis-related lncRNA signature employing univariate, Least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analyses, survival and nomogram analyses ensued to scrutinize the predictive value of the prognostic signature.

Results: The segregation of patients into two risk groups was done by the constructed pyroptosis-related-lncRNA signature (encompassing 14 lncRNAs). The prognosis was poorer for individuals of the high-risk group versus (vs.) the low-risk group with the risk score emerging as an independent prognostic factor by regression analyses. The accuracy of this signature was corroborated by Receiver operating characteristic curve (ROC) analysis with the three-years area under time-dependent ROC curve (AUC) reaching 0.767. Further analyses unveiled a conspicuous enrichment of immune-related pathways in the low-risk group. The high-risk group demonstrated an immunologically "cold" profile based on the immune cell infiltration landscape.

Conclusions: The lncRNA signature encompassing 14 pyroptosis-related lncRNAs could be a prognostic marker for HNSCC, suggesting pyroptosis might be a promising therapeutic target in HNSCC.
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http://dx.doi.org/10.1016/j.intimp.2021.108268DOI Listing
October 2021

Optimizing SnO /Fe O Hetero-Nanocrystals Toward Rapid and Highly Reversible Lithium Storage.

Small 2021 Oct 22:e2103532. Epub 2021 Oct 22.

Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Engineering oxygen vacancy and boosting Li O reversibility on oxides-based electrode are of significance but remains a challenge in high-power lithium-ion batteries. Herein, the heterogenous SnO /Fe O nanocrystals are demonstrated with tailorable x and y values enabled by a glucose-assisted spray combustion technique. Density functional theory calculations unveil the SnO /Fe O with a maximum x value has the optimal electronic structure, the metallic Fe generated from Fe O can markedly reduce the free energy to break Li-O bonds for accelerating subsequent delithiation process of Li O. Consequently, the optimized SnO /Fe O exhibits a remarkably enhanced electrochemical reversibility and reaction kinetics. After stabilized by reduced graphene oxide, the hybrid delivers a high reversible specific capacity of 1113 mAh g with superior rate performance (474 mAh g at 20 A g ) and long cycle life (negligible loss after 500 cycles at 5 A g ), the oxygen vacancy and microstructure are well-maintained after cycles. This work provides the possibilities for skillfully regulating oxygen vacancy and meantime enhancing Li O reversibility.
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http://dx.doi.org/10.1002/smll.202103532DOI Listing
October 2021

Cloning and identification of a new repressor of 3,17β-Hydroxysteroid dehydrogenase of Comamonas testosteroni.

Mol Biol Rep 2021 Oct 22. Epub 2021 Oct 22.

School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.

Background: 3,17β-hydroxysteroid dehydrogenase (3,17β-HSD) is a key enzyme in the metabolic pathway for steroid compounds catabolism in Comamonas testosteroni. Tetracycline repressor (TetR) family, repressors existing in most microorganisms, may play key roles in regulating the expression of 3,17β-HSD. Previous reports showed that three tetR genes are located in the contig58 of C. testosteroni ATCC 11996 (GenBank: AHIL01000049.1), among which the first tetR gene encoded a potential repressor of 3,17β-HSD by sensing environmental signals. However, whether the other proposed tetR genes act as repressors of 3,17β-HSD are still unknown.

Methods And Results: In the present study, we cloned the second tetR gene and analyzed the regulatory mechanism of the protein on 3,17β-HSD using electrophoretic mobility shift assay (EMSA), gold nanoparticles (AuNPs)-based assay, and loss-of-function analysis. The results showed that the second tetR gene was 660-bp, encoding a 26 kD protein, which could regulate the expression of 3,17β-HSD gene via binding to the conserved consensus sequences located 1100-bp upstream of the 3,17β-HSD gene. Furthermore, the mutant strain of C. testosteroni with the second tetR gene knocked-out mutant expresses good biological genetic stability, and the expression of 3,17β-HSD in the mutant strain is slightly higher than that in the wild type under testosterone induction.

Conclusions: The second tetR gene acts as a negative regulator in 3,17β-HSD expression, and the mutant has potential application in bioremediation of steroids contaminated environment.
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http://dx.doi.org/10.1007/s11033-021-06566-9DOI Listing
October 2021

Evidence and assessment of parenchymal patterns of ultrasonography for breast cancer detection among Chinese women: a cross-sectional study.

BMC Med Imaging 2021 Oct 19;21(1):152. Epub 2021 Oct 19.

Department of Ultrasound, First Affiliated Hospital of Fujian Medical University, No 20 Cha zhong Road, Taijiang District, Fuzhou, 350000, Fujian, China.

Background: Screening of breast cancer in asymptomatic women is important to evaluate for early diagnosis. In China ultrasound is a more frequently used method than mammography for the detection of breast cancer. The objectives of the study were to provide evidence and assessment of parenchymal patterns of ultrasonography for breast cancer detection among Chinese women.

Methods: Breast ultrasound examinations including the parenchymatous pattern of cytopathological confirmed breast cancer (n = 541) and age-matched cytopathological not confirmed breast cancer (n = 849) women were retrospectively reviewed by seven sonographer physicians. According to compositions of ducts, the thickness of the breast, diameter of ducts, fat lobules, and fibro glandular tissues, the breast parenchymatous pattern was categorized into heterogeneous (high percentage of fatty tissues), ductal (the inner diameters of ducts > 50% of the thick mass of the breast), mixed (the inner diameters of ducts was 50% of the thick mass of the breast), and fibrous categories (a dense classification of the breast).

Results: Heterogeneous (p < 0.0001, OR = 3.972) and fibrous categories (p < 0.0001, OR = 2.702) were higher among women who have cytopathological confirmed breast cancer than those who have not cytopathological confirmed breast cancer. The heterogeneous category was high-risk ultrasonographic examination category followed by the fibrous category. Agreements between sonographer physicians for categories of ultrasonic examinations were fair to good (Cohen's k = 0.591).

Conclusions: Breast cancer risk in Chinese asymptomatic women differ according to the ultrasonographic breast parenchymal pattern.

Level Of Evidence: III. Technical efficacy stage: 2.
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http://dx.doi.org/10.1186/s12880-021-00687-0DOI Listing
October 2021

Effects of CEO Identity on Non-family Managers' Pay Dispersion in Family Firms: A Social Comparison Perspective.

Front Psychol 2021 1;12:683011. Epub 2021 Oct 1.

Business School, Zhejiang University City College, Hangzhou, China.

The fairness of compensation has been a prominent focus for non-family managers, and pay dispersion, which reflects compensation fairness, has attracted much attention from scholars. Based on social comparison theory, this study investigates the factors that affect the pay dispersion between CEO and non-family managers. In family firms, the role of CEO, which is central in corporate governance, can be filled by either a family or a non-family member. This study provides insights into how the identity of the CEO affects pay dispersion and investigates the moderating effects of CEO tenure and institutional environment. Using the data of Chinese listed family firms from 2009 to 2015, the results show that the presence of non-family CEOs could decrease the pay dispersion between CEO and non-family managers. Empirical evidence also supports that the negative relationship between CEO identity and pay dispersion weakens when CEO tenure increases and the institutional environment matures.
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http://dx.doi.org/10.3389/fpsyg.2021.683011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517182PMC
October 2021

Fluorescent noble metal nanoclusters for contaminants analysis in food matrix.

Crit Rev Food Sci Nutr 2021 Oct 16:1-19. Epub 2021 Oct 16.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.

Recently, food safety issues caused by contaminants have aroused great public concern. The development of innovative and efficient sensing techniques for contaminants detection in food matrix is in urgent demand. As fluorescent nanomaterials, noble metal nanoclusters have attracted much attention because of their ease of synthesis, enhanced catalytic activity and biocompatibility, and most importantly, excellent photoluminescence property that provides promising analytical applications. This review comprehensively introduced the synthesis method of noble metal nanoclusters, and summarized the application of metal nanoclusters as fluorescent sensing materials in the detection of pollutants, including pesticides, heavy metal, mycotoxin, food additives, and other contaminants in food. The detection mechanism of pesticide residues mostly relies on the inhibition of natural enzymes. For heavy metals, the detection mechanism is mainly related to the interaction between metal ions and nanoclusters or ligands. It is evidenced that metal nanoclusters have great potential application in the field of food safety monitoring. Moreover, challenges and future trends of nanoclusters were discussed. We hope that this review can provide insights and directions for the application of nanoclusters in contaminants detection.
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http://dx.doi.org/10.1080/10408398.2021.1990010DOI Listing
October 2021

A genome-wide association study in Chinese identifies novel risk loci for Behcet's uveitis.

Arthritis Rheumatol 2021 Oct 15. Epub 2021 Oct 15.

The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, China.

Purpose: To explore susceptibility loci associated with uveitis in Behcet's disease (BD).

Methods: We conducted a genome-wide association study (GWAS) primarily involving 978 BD uveitis cases and 4388 controls and a replication study of 953 BD uveitis cases and 2129 controls in the Chinese population. Luciferase reporter analysis and Chromatin immunoprecipitation (ChIP) assay were performed to explore the functional role of susceptibility genetic variants nearby ZMIZ1.

Results: Three independent HLA alleles (HLA-B51, HLA-A26 and HLA-C0704) were identified in a genome-wide association with BD uveitis. In the non-HLA region, besides confirming 7 previously reported loci, we identified 22 novel susceptibility variants located in 16 loci. Meta-analysis of the Chinese cohort involving 1931 cases and 6517 controls and a published Japanese cohort of 611 cases and 737 controls showed genome-wide significant associations with ZMIZ1, RPS6KA4, IL10RA, SIPA1-FIBP-FOSL1 and VAMP1. Functional experiments demonstrated that genetic variants of ZMIZ1 were associated with an enhanced transcriptional activity and an increased expression of ZMIZ1.

Conclusions: This GWAS study identified a novel set of genetic variants that are associated with susceptibility to uveitis in BD. These findings enriched our understanding of the contribution of genetic factors to the disease.
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http://dx.doi.org/10.1002/art.41998DOI Listing
October 2021

Minimally Invasive Surgery for Suspected Early-Stage Ovarian Cancer; A Cost-Effectiveness Study.

BJOG 2021 Oct 15. Epub 2021 Oct 15.

Columbia University College of Physicians and Surgeons.

Objective: While there are a number of benefits to minimally invasive surgery (MIS) for women with ovarian cysts, there is an increased risk of ovarian capsule rupture during the procedure, which could potentially seed the abdominal cavity with malignant cells. We developed a decision model to compare the risks, benefits, effectiveness and cost of MIS versus laparotomy in women with ovarian masses.

Design: Cost-effectiveness study POPULATION: Hypothetical cohort of 10,000 women with ovarian masses who were undergoing surgical management.

Methods: The initial decision point in the model was performance of surgery via laparotomy or a MIS approach. Model probabilities, costs and utility values were derived from published literature and administrative data sources. Extensive sensitivity analyses were conducted to assess the robustness of the findings.

Main Outcome Measures: The primary outcome was the cost-effectiveness of MIS versus laparotomy for women with a pelvic mass measured by incremental cost-effectiveness ratios (ICERs).

Results: MIS was the least costly strategy at $7,732 per women on average, compared with $17,899 for laparotomy. In our hypothetical cohort of 10,000 women, there were 64 cases of ovarian rupture in the MIS group and 53 in the laparotomy group, while there were 26 cancer related deaths in the MIS group and 25 in the laparotomy group. MIS was more effective than laparotomy (188,462 QALYs for MIS versus 187,631 QALYs for laparotomy). Thus, MIS was a dominant strategy, being both less costly and more effective than laparotomy. These results were robust in a variety of sensitivity analyses.

Conclusion: MIS constitutes a cost-effective management strategy for women with suspicious ovarian masses.
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http://dx.doi.org/10.1111/1471-0528.16967DOI Listing
October 2021

Autoantibody of interleukin-17A induced by recombinant attenuates airway inflammation in mice with neutrophilic asthma.

J Asthma 2021 Oct 13:1-11. Epub 2021 Oct 13.

Department of Pediatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

: Previous studies have shown Interleukin (IL)-17A as an important contributor to the development of severe asthma, which is mainly characterized by neutrophilic inflammation and less response to corticosteroids. Consequently, the IL-17A-neutrophil axis could be a potential therapeutic target. Previously, we constructed a recombinant (rMS) expressing fusion protein Ag85A-IL-17A, and confirmed it could induce production of IL-17A autoantibody . This study uses a murine model of neutrophilic asthma to further investigate the effects of rMS on airway inflammation. DO11.10 mice were divided into four groups: phosphate buffered saline (PBS), asthma, rMS and MS. This murine model of neutrophilic asthma was established with ovalbumin (OVA) challenge, whereby PBS, rMS and MS were administered intranasally. Anti-inflammatory effects on inflammatory cell infiltration and expression of inflammatory mediators in bronchoalveolar lavage fluid (BALF) were evaluated, along with histopathological changes in lung tissues. A sustained high-titer IL-17A autoantibody was detected in sera of the rMS group. Compared to the asthma group, the number of neutrophils, IL-17A, CXCL-1 levels and MPO activity in the rMS group were all significantly reduced ( < 0.01). Histological analysis showed rMS remarkably suppressed inflammatory infiltration around bronchia. The inflammation score and the mucus score in the rMS group were both significantly lower than those in the asthma group ( < 0.001). : rMS ameliorated airway inflammation in mice with neutrophilic asthma caused by inducing IL-17A autoantibody and regulating the IL-17A-neutrophil axis, thus offering a possible novel treatment for neutrophilic asthma.
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http://dx.doi.org/10.1080/02770903.2021.1989696DOI Listing
October 2021

Nogo-A Induced Polymerization of Microtubule Is Involved in the Inflammatory Heat Hyperalgesia in Rat Dorsal Root Ganglion Neurons.

Int J Mol Sci 2021 Sep 26;22(19). Epub 2021 Sep 26.

Department of Human Anatomy, Histology & Embryology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

The microtubule, a major constituent of cytoskeletons, was shown to bind and interact with transient receptor potential vanilloid subfamily member 1 (TRPV1), and serves a pivotal role to produce thermal hyperalgesia in inflammatory pain. Nogo-A is a modulator of microtubule assembly and plays a key role in maintaining the function of TRPV1 in inflammatory heat pain. However, whether the microtubule dynamics modulated by Nogo-A in dorsal root ganglion (DRG) neurons participate in the inflammatory pain is not elucidated. Here we reported that the polymerization of microtubules in the DRG neurons, as indicated by the acetylated α-tubulin, tubulin polymerization-promoting protein 3 (TPPP3), and microtubule numbers, was significantly elevated in the complete Freund's adjuvant (CFA) induced inflammatory pain. Consistent with our previous results, knock-out (KO) of Nogo-A protein significantly attenuated the heat hyperalgesia 72 h after CFA injection and decreased the microtubule polymerization via up-regulation of phosphorylation of collapsin response mediator protein 2 (CRMP2) in DRG. The colocalization of acetylated α-tubulin and TRPV1 in DRG neurons was also reduced dramatically in Nogo-A KO rats under inflammatory pain. Moreover, the down-regulation of TRPV1 in DRG of Nogo-A KO rats after injection of CFA was reversed by intrathecal injection of paclitaxel, a microtubule stabilizer. Furthermore, intrathecal injection of nocodazole (a microtubule disruptor) attenuated significantly the CFA-induced inflammatory heat hyperalgesia and the mechanical pain in a rat model of spared nerve injury (SNI). In these SNI cases, the Nogo-A and acetylated α-tubulin in DRG were also significantly up-regulated. We conclude that the polymerization of microtubules promoted by Nogo-A in DRG contributes to the development of inflammatory heat hyperalgesia mediated by TRPV1.
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http://dx.doi.org/10.3390/ijms221910360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508904PMC
September 2021

Superhigh co-adsorption of tetracycline and copper by the ultrathin g-CN modified graphene oxide hydrogels.

J Hazard Mater 2021 Sep 29;424(Pt B):127362. Epub 2021 Sep 29.

Guangzhou Key Laboratory Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.

Development of economic and efficient absorbent for the simultaneous removal of antibiotics and heavy metals is needed. In this study, a three-dimensional porous ultrathin g-CN (UCN) /graphene oxide (GO) hydrogel (UCN-GH) was prepared by co-assembling of UCN and GO nanosheets via the facile hydrothermal reaction. Characterizations indicated that the addition of UCN significantly decreased the reduction of CO and O-CO related groups of GO during the hydrothermal reaction and introduced amine groups on UCN-GH. The UCN-GH exhibited excellent ability on the co-removal of Cu(II) (q = 2.0-2.5 mmol g) and tetracycline (TC) (q = 1.2-3.0 mmol g) from water. The adsorption capacities were increased as UCN mass ratio increasing. The mutual effects between Cu(II) and TC were examined through adsorption kinetics and isotherm models. Characterizations and computational chemistry analysis indicated that Cu(II) is apt to coordinate with the amine groups on UCN than with oxygen groups on GO, which accounts for the enhanced adsorption ability of UCN-GH. In the binary system, Cu(II) acts as a bridge between TC and UCN-GH enhanced the removal of TC. The effects of pH and regular salt ions on the removal of Cu(II)/TC were examined. Moreover, the prepared UCN-GH also showed comparable co-adsorption capacities in practical water/wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127362DOI Listing
September 2021

TRPV4 inhibitor HC067047 produces antidepressant-like effect in LPS-induced depression mouse model.

Neuropharmacology 2021 Oct 9;201:108834. Epub 2021 Oct 9.

Clinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, 421001, Hengyang, Hunan, China; Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, 571199, Haikou, China. Electronic address:

Inflammation is a crucial component that contributes to the pathogenesis of major depressive disorder. It has been revealed that the nonselective cation channel transient receptor potential vanilloid 4 (TRPV4) profoundly affects a variety of physiological processes, including inflammation. However, its roles and mechanisms in LPS-induced depression are still unclear. Here, for the first time, we found that there was a significant increase in TRPV4 in the hippocampus in a depression mouse model induced by LPS. TRPV4 inhibitor HC067047 or knockdown the hippocampal TRPV4 with TRPV4 shRNA could effectively rescue the aberrant behaviors. Furthermore, TRPV4 inhibitor HC067047 reduced the activation of astrocyte and microglia, decreased expression of CaMKII-NLRP3 inflammasome and increased the expression of neurogenesis marker DCX in the hippocampus. In addition, enhanced neuroinflammation in the serum was also reversed by TRPV4 inhibitor HC067047. Thus, we consider that TRPV4 has an important role in contributing to the depression-like behavior following LPS-induced systemic inflammation.
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http://dx.doi.org/10.1016/j.neuropharm.2021.108834DOI Listing
October 2021

Combining contrast-enhanced ultrasound and blood cell analysis to improve diagnostic accuracy of plasma cell mastitis.

Exp Biol Med (Maywood) 2021 Oct 9:15353702211049361. Epub 2021 Oct 9.

Center of Clinical Laboratory, Dushu Lake Hospital Affiliated to Soochow University, Suzhou 215000, China.

Plasma cell mastitis is a benign suppurative disease of the breast, lack of specific clinical manifestations, which is easy to be misdiagnosed and mistreated, often confused with mastitis, breast cancer (BC), and other diseases. Thus, we aimed to establish a combined model of promoting diagnostic accuracy of plasma cell mastitis by contrast-enhanced ultrasound (CEUS) patterns and routine blood cell analysis. Eighty-eight plasma cell mastitis, 91 breast cancer, and 152 other benign breast diseases' patients grouped according to pathological diagnosis underwent CEUS and blood cell analysis examination; 100 healthy female donors were involved. All the plasma cell mastitis and breast cancer patients presented hyperenhancement of CEUS breast lesions compared with others. The majority of plasma cell mastitis (65/88) showed perfusion defect of CEUS patterns with smooth edge (56/65) and multiple lesions (49/65); in contrast, fewer breast cancer patients (30/91) displayed perfusion defect. White blood cell count (WBC), neutrophils, and neutrophils/lymphocytes ratio of blood cell analysis in plasma cell mastitis patients increased significantly compared with other patients ( < 0.0001). Combining perfusion defect of CEUS patterns and WBC yielded an area under the receiver operating characteristic curve of 0.831, higher than single 0.720 and 0.774, respectively. The cut-off value of WBC (7.28 × 10/L) helped remaining 65.2% (15/23) atypical cases to be correctly diagnosed as plasma cell mastitis, not misdiagnosed as breast cancer. In conclusion, CEUS presented a clear perfusion defect pattern of plasma cell mastitis lesion for the first time. A precise WBC by routine blood cell analysis test can assist CEUS examination in the differential diagnosis of plasma cell mastitis and breast cancer. It is a promised combination for laboratory diagnostic of PCM.
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http://dx.doi.org/10.1177/15353702211049361DOI Listing
October 2021

Light-Motivated SnO /TiO Heterojunctions Enabling the Breakthrough in Energy Density for Lithium-Ion Batteries.

Adv Mater 2021 Oct 8:e2103558. Epub 2021 Oct 8.

Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science & Technology, Shanghai, 200237, China.

Powering lithium-ion batteries (LIBs) by light-irradiation will bring a paradigm shift in energy-storage technologies. Herein, a photoaccelerated rechargeable LIB employing SnO /TiO heterojunction nanoarrays as a multifunctional anode is developed. The electron-hole pairs generated by the Li TiO (x ≥ 0) under light irradiation synergistically enhance the lithiation kinetics and electrochemical reversibility of both SnO and TiO . Specifically, the electrons can quickly pour into the SnO and the generated Sn due to the more positive conduction band potentials (vs TiO ), and mean while the holes also promote the intercalation of Li into TiO by reaching charge balance. A remarkable increase in areal specific capacity is therefore achieved from 1.91 to 3.47 mAh cm at 5 mA cm . More impressively, there is no capacity loss even through 100 cycles, which is the best report for photorechargeable LIBs to date, owing to the strong and stable photoresponse current. This finding exhibits a feasible pathway to break the limitation in the energy density of LIBs by the efficient conversion and storage of solar energy.
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http://dx.doi.org/10.1002/adma.202103558DOI Listing
October 2021

Effect of HFY03 on the Antifatigue and Antioxidation Ability of Running Exhausted Mice.

Oxid Med Cell Longev 2021 28;2021:8013681. Epub 2021 Sep 28.

Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.

Yak yogurt is mainly produced in Qinghai-Tibet Plateau. It is a kind of naturally fermented dairy product. It contains abundant microorganisms. (LF) HFY03 is a lactic acid bacteria derived from it. Our main research content is to study the influence of LF-HFY03 on the antifatigue and antioxidation ability of running exhausted mice. We gave different doses of LF-HFY03 to mice by gavage for 4 weeks. We selected vitamin C as the positive control group, mainly to study the relationship between antioxidant capacity and fatigue resistance and LF-HFY03 in mice with running exhaustion. The results showed that LF-HFY03 and vitamin C could significantly improve the running time of mice. And with the increase in LF-HFY03 concentration, the exhaustion time of mice was also extended. LF-HFY03 can reduce the content of urea nitrogen and lactic acid and also can increase the content of free fatty acids and liver glycogen. The levels of alanine aminotransferase, serum creatine kinase, and aspartate aminotransferase in mice decreased gradually as the antioxidant peptide level of walnut albumin increased. LF-HFY03 can reduce malondialdehyde (MDA) levels in a quantification-dependent manner and can also increase catalase (CAT) and superoxide dismutase (SOD) levels. LF-HFY03 can also increase the expressions of CAT mRNA, Cu/Zn-SOD, and Mn-SOD in the liver of mice. At the same time, LF-HFY03 can also increase the expression of protein of threonine transporter 1 (AST1)/alanine/cysteine/serine, mRNA, nNOS, and eNOS. At the same time, the solution could reduce the expression of TNF-, syncytin-1, and inducible nitric oxide synthase (iNOS). The results showed that LF-HFY03 has a high development and application prospect as an antifatigue probiotic nutritional supplement.
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http://dx.doi.org/10.1155/2021/8013681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492249PMC
September 2021

Soybean (Glycine max L Merr) host-plant defenses and resistance to the two-spotted spider mite (Tetranychus urticae Koch).

PLoS One 2021 7;16(10):e0258198. Epub 2021 Oct 7.

Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, Canada.

In southern Ontario, Canada, the two-spotted spider mite (Tetranychus urticae) is an emerging pest of soybean (Glycine max) due to the increasing incidence of warmer, drier weather conditions. One key strategy to manage soybean pests is breeding resistant cultivars. Resistance to pathogens and herbivores in soybean has been associated with isoflavonoid phytoalexins, a group of specialized metabolites commonly associated with root, leaf and seed tissues. A survey of 18 Ontario soybean cultivars for spider mite resistance included evaluations of antibiosis and tolerance in relation to isoflavonoid and other metabolites detected in the leaves. Ten-day and 4-week trials beginning with early growth stage plants were used to compare survival, growth, fecundity as well as damage to leaves. Two-spotted spider mite (TSSM) counts were correlated with HPLC measurements of isoflavonoid concentration in the leaves and global metabolite profiling by high resolution LC-MS to identify other metabolites unique to the most resistant (R) and susceptible (S) cultivars. Within 10 days, no significant difference (P>0.05) in resistance to TSSM was determined between cultivars, but after 4 weeks, one cultivar, OAC Avatar, was revealed to have the lowest number of adult TSSMs and their eggs. Other cultivars showing partial resistance included OAC Wallace and OAC Lakeview, while Pagoda was the most tolerant to TSSM feeding. A low, positive correlation between isoflavonoid concentrations and TSSM counts and feeding damage indicated these compounds alone do not explain the range of resistance or tolerance observed. In contrast, other metabolite features were significantly different (P<0.05) in R versus S cultivars. In the presence of TSSM, the R cultivars had significantly greater (P<0.05) concentrations of the free amino acids Trp, Val, Thr, Glu, Asp and His relative to S cultivars. Furthermore, the R cultivar metabolites detected are viable targets for more in-depth analysis of their potential roles in TSSM defense.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258198PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496822PMC
October 2021

Effects of delayed weather radar images on pilots' spatial awareness.

Appl Ergon 2021 Oct 1;98:103598. Epub 2021 Oct 1.

School of Psychology, Georgia Institute of Technology, Atlanta, GA, USA.

Data-linked Next Generation Weather Radar (NEXRAD) images can be delayed up to 20 min in the cockpit. Pilots' underappreciating or ignoring the time delay may be the major cause of two fatal accidents. No studies have connected spatial awareness with accidents. This study evaluated how delayed radar information affects the spatial awareness of pilots at three levels of analysis. Thirty-one student pilots and flight instructors completed three sequential estimation tasks (i.e., the current location of storms, the current relative distance to storms, and the future relative distance to storms). Fifty-four weather scenarios were developed for three factors (storm speeds, delays, displays) and presented to pilots. The results indicated that delays and the storm speed significantly affected the three levels of spatial awareness. Participants' estimation accuracy was the lowest under long delay and fast speed in the current location estimation, under medium delay and speed in the current distance estimation, and under short delay and slow speed in the future distance estimation. Spatial awareness could be high under the long delay and fast speed conditions if pilots had no time limits. Thus, pilots can process 20-min delayed radar information. However, there were no differences in estimation accuracy between the static and animation displays in any of the conditions. Well-designed features on displays, such as scale or distance measuring tools, can aid pilots' spatial estimation and support all levels of spatial awareness.
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http://dx.doi.org/10.1016/j.apergo.2021.103598DOI Listing
October 2021

Effect of NPC1L1 polymorphism on warfarin stable dose in Chinese patients under heart valve replacement surgery.

Clin Exp Pharmacol Physiol 2021 Oct 4. Epub 2021 Oct 4.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.

Warfarin is the most often anticoagulant choice for preventable thromboembolism. Notably, vitamin K plays a vital role in the process of warfarin's anticoagulant effect. Therefore, we presume NPC1L1, a key transporter of vitamin K (VK) intestinal absorption, may modulate the anticoagulant effect of warfarin. Studies have shown that NPC1L1(-762T>C, rs2073548) and p53 (P72R, rs1042522) variations are implicated in influencing NPC1L1 expression. This study aimed to assess the association between these two variants and warfarin stable dose (WSD). A two-stage extreme phenotype design was used to explore the influence of these two variants (rs2073548, rs1042522) on WSD variance in 655 Chinese patients undergoing heart valve replacement surgery. NPC1L1 rs2073548, p53 rs1042522, VKORC1 rs9923231 and CYP2C9*1/*3 polymorphisms were genotyped by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) or Sanger sequencing, respectively. WSD was identified when target monitoring international normalized ratio (INR) value at 2.0-3.0. In the discovery phase, NPC1L1 rs2073548 A allele carriers occupied a significantly higher rate in the low dose group (P = .019). However, in the validation group, warfarin dosage in patients with the rs2073548 AA, AG and GG genotypes were 2.91 ± 0.97 mg/day, 3.02 ± 1.00 mg/day and 3.00 ± 1.06 mg/day, respectively. Multiple linear regression analysis results suggested that CYP2C9*3 and VKORC1 rs9923231, but not NPC1L1 rs2073548, were independent predictors of WSD in Chinese heart valve replacement (HVR) surgical patients.
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http://dx.doi.org/10.1111/1440-1681.13599DOI Listing
October 2021

TiO NPs induce the reproductive toxicity in mice with gestational diabetes mellitus through the effects on the endoplasmic reticulum stress signaling pathway.

Ecotoxicol Environ Saf 2021 Dec 27;226:112814. Epub 2021 Sep 27.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China. Electronic address:

The effect of one of the most widely studied nanomaterials at present, TiO nanoparticles (NPs), on pregnancy-related diseases is not clear. In this study, the adverse effects of TiO NPs on mice with gestational diabetes mellitus (GDM) and their possible mechanism were investigated. GDM mice were orally administered 0, 10, 50 and 250 mg/kg TiO NPs for 14 days. GDM reduced the weight of pregnant mice, destroyed the placental structure and caused abnormal fetal development. After exposure to increasing doses of TiO NPs, blood glucose levels increased significantly and body weight further decreased in GDM mice. The accumulation of the Ti content was detected in the placenta and fetus, which may further damage the placental structure in GDM mice, thereby exacerbating abnormal fetal development. In addition, the MDA and SOD activities were obviously increased, and the expression of genes associated with endoplasmic reticulum stress (ERS) (PERK, eIF2α, AFT4, IRE1α, and XBP1s) and apoptosis (CHOP, JNK, Bax/Bcl-2, Caspase-12, Caspase-9, and Caspase-3) were also obviously increased in the placenta, which reflected the possible activation of apoptosis. It could be speculated that the reproductive toxicity of TiO NPs in GDM mice triggered oxidative stress that subsequently activated ERS pathways to induce cell apoptosis.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112814DOI Listing
December 2021

Reduced blood flow velocity in lenticulostriate arteries of patients with CADASIL assessed by PC-MRA at 7T.

J Neurol Neurosurg Psychiatry 2021 Sep 28. Epub 2021 Sep 28.

State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

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http://dx.doi.org/10.1136/jnnp-2021-326258DOI Listing
September 2021

Two new sesquiterpenes from .

Nat Prod Res 2021 Sep 29:1-6. Epub 2021 Sep 29.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.

Two new sesquiterpenes (-) and six known analogues (-) were isolated from the branches and leaves of Pierre. The structures of the new compounds were identified by analyzing 1 D and 2 D NMR data and HRESIMS data, combined with induced and calculated circular dichroism experiments. In addition, compounds - and showed notable nitric oxide (NO) inhibitory effects (IC < 10 M) on the model of the lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.
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http://dx.doi.org/10.1080/14786419.2021.1984907DOI Listing
September 2021

Dual Blockade of Lactate/GPR81 and PD-1/PD-L1 Pathways Enhances the Anti-Tumor Effects of Metformin.

Biomolecules 2021 Sep 17;11(9). Epub 2021 Sep 17.

School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, China.

Metformin is a widely used antidiabetic drug for cancer prevention and treatment. However, the overproduction of lactic acid and its inefficiency in cancer therapy limit its application. Here, we demonstrate the synergistic effects of the lactate/GPR81 blockade (3-hydroxy-butyrate, 3-OBA) and metformin on inhibiting cancer cells growth in vitro. Simultaneously, this combination could inhibit glycolysis and OXPHOS metabolism, as well as inhibiting tumor growth and reducing serum lactate levels in tumor-bearing mice. Interestingly, we observed that this combination could enhance the functions of Jurkat cells in vitro and CD8 T cells in vivo. In addition, considering that 3-OBA could recover the inhibitory effects of metformin on PD-1 expression, we further determined the dual blockade effects of PD-1/PD-L1 and lactate/GPR81 on the antitumor activity of metformin. Our results suggested that this dual blockade strategy could remarkably enhance the anti-tumor effects of metformin, or even lead to tumor regression. In conclusion, our study has proposed a novel and robust strategy for a future application of metformin in cancer treatment.
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http://dx.doi.org/10.3390/biom11091373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466555PMC
September 2021

Genome-wide Association Study of Lipid Traits in Youth With Type 2 Diabetes.

J Endocr Soc 2021 Nov 18;5(11):bvab139. Epub 2021 Aug 18.

Division of Pediatric Endocrinology, University of California at San Francisco, San Francisco, CA, USA.

Context: Dyslipidemia is highly prevalent in youth with type 2 diabetes (T2D), yet the pathogenic components of dyslipidemia in youth with T2D are poorly understood.

Objective: To evaluate the genetic determinants of lipid traits in youth with T2D through a genome-wide association study.

Design Participants And Main Outcome Measures: We genotyped 206 928 variants and imputed 17 642 824 variants in 1076 youth (mean age 15.0 ± 2.48 years) with T2D from the Treatment Options for Type 2 Diabetes in Adolescents and Youth (TODAY) and SEARCH for Diabetes in Youth (SEARCH) studies as part of the Progress in Diabetes Genetics in Youth (ProDiGY) consortium. We performed association testing for triglyceride and low-density lipoprotein cholesterol and high-density lipoprotein cholesterol (HDL-c) concentrations adjusted for the genetic relationship matrix within each substudy followed by meta-analyses for each trait.

Results: We identified a novel association between a deletion on chromosome 3 (3:67817380_AT/A_Deletion:RP11-81N13.1) and triglyceride levels at genome-wide level of significance ( = 2.3 × 10) with each risk allele increasing triglycerides by 20%. We also identified a genome-wide significant signal at rs247617 ( = 5.1 × 10) between and associated with HDL-c, with carriers of 1 copy of the risk allele having twice higher HDL-c.

Conclusions: Our genetic analyses of lipid traits in youth with T2D have identified 1 novel and 1 previously known locus. Additional studies are needed to further characterize the genetic architecture of dyslipidemia in youth with T2D.
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http://dx.doi.org/10.1210/jendso/bvab139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459445PMC
November 2021

Development changes in multi-scale structure and functional properties of waxy corn starch at different stages of kernel growth.

Int J Biol Macromol 2021 Sep 21;191:335-343. Epub 2021 Sep 21.

International Institute for Nanocomposites Manufacturing (IINM), WMG, University of Warwick, Coventry CV4 7AL, United Kingdom. Electronic address:

Waxy corn starch is widely used in food and papermaking industries due to its unique properties. In this work, the structural and functional properties of starch isolated from waxy corn at different stages of kernel growth were investigated and their relationships were clarified. The results showed that with kernel growth, the surface of starch granules became smooth gradually, and the inner growth rings and the porous structure grew and became clear. Meanwhile, the weight-average molecular mass (M), root mean square radius (R), and average particle size increased while the amylose content decreased, which should account for the decreased pasting temperature (from 71.37 to 67.44 °C) and increased peak viscosity (1574.2 to 1883.1 cp) and breakdown value observed. Besides, the contents of slowly digestible starch (SDS) and resistant starch (RS) in waxy corn starch decreased significantly (from 44.01% to 40.88% and from 16.73% to 9.80%, respectively, p < 0.05) due to decreases in the double helix content, crystallinity, and structural order, and increases in the semi-crystalline lamellae thickness and the amorphous content. This research provides basic data for the rational utilization of waxy corn starch at different stages of kernel growth.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.120DOI Listing
September 2021

A limited number of slices yields comparable results to all slices in foot intrinsic muscle deterioration ratio on computed tomography and magnetic resonance imaging.

J Biomech 2021 Sep 13;129:110750. Epub 2021 Sep 13.

Program in Physical Therapy, Washington University School of Medicine in St. Louis, United States. Electronic address:

Diagnostic imaging modalities, like computed tomography (CT) and magnetic resonance imaging (MRI), can be used to assess in vivo muscle quality. Quantitative assessment using these techniques is time-intensive and costly due in part to extensive post-processing needs. The purpose of this study was to identify whether a subset of slices on CT and MRI would yield comparable results to the full number of slices for a measure of muscle quality (muscle deterioration ratio = fat volume/muscle volume) in the foot intrinsic muscles of people with diabetes and peripheral neuropathy. CT (0.6 mm slice thickness) and MRI (3.5 mm slice thickness) scans were obtained using previously described methods. The total number of slices acquired during the scan was compared to several conditions using a portion of slices. Bland-Altman plots and Lin's concordance correlation coefficient were used to test agreement. Any condition using at least three slices yielded substantial to almost perfect agreement with the total number of slices on both CT and MRI (Range of Lin's concordance correlation coefficient: 0.947-0.999). Using a single slice in the middle of the region of interest demonstrated poor to moderate agreement with the total number of slices. The findings of this study suggest that using a limited number of slices to quantify muscle deterioration ratio on CT or MRI is a viable way to balance the combined need for measurement accuracy with feasibility in research and clinical settings.
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http://dx.doi.org/10.1016/j.jbiomech.2021.110750DOI Listing
September 2021

Bio-informatics and Experiments Reveal the Mechanism of Schisandrin A Against MDA-MB-231 cells.

Bioengineered 2021 Sep 22. Epub 2021 Sep 22.

Department of Pharmacy, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.

Schisandrin A (SchA) has been reported to have good anti-cancer effects. However, its anti-cancer mechanism in breast cancer remains unknown. This study aimed to explore the mechanism of SchA in breast cancer treatment using bio-informatics analysis and experiments. The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Cards, and PharmMapper databases were used to screen the candidate targets of SchA against MDA-MB-231 cells selected as the tested cell line through MTT analysis. The functions and pathways of the targets were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and further analyzed using DAVID 6.8.1 database. Network pharmacology analysis revealed 77 candidate targets, 31 signal pathways, and 208 GO entries (P < 0.05). The targets regulated serine-type endopeptidase and protein tyrosine kinase activities, thereby promoting the migration and inhibiting the apoptosis of MDA-MB-231 cells. Comprehensive analysis of the "Protein-Protein Interaction" (PPI) and "Component-Targets-Pathways" (C-T-P) networks constructed using Cytoscape 3.7.1 software revealed four core targets: EGFR, PIK3R1, MMP9 and Caspase 3. Their docking scores with SchA were subsequently investigated through molecular docking. The wound healing, Hoechst 33342/PI, and western blot assays confirmed that SchA significantly down-regulated EGFR, PIK3R1, and MMP9, but up-regulated cleaved-caspase 3, thus inhibiting the migration and promoting the apoptosis of MDA-MB-231 cells. Reckoning the findings of the study, SchA is a potential adjuvant treatment for breast cancer.
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http://dx.doi.org/10.1080/21655979.2021.1982307DOI Listing
September 2021
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