Publications by authors named "Chen He"

657 Publications

Exposure to nanopolystyrene and its 4 chemically modified derivatives at predicted environmental concentrations causes differently regulatory mechanisms in nematode Caenorhabditis elegans.

Chemosphere 2022 Jun 28:135498. Epub 2022 Jun 28.

The State Key Laboratory of Translational Medicine and Innovative Drug Development, Jiangsu Simcere Diagnostics Co., Ltd., Nanjing, 210009, China.

Nanoplastics represented by nanopolystyrene (NPS) and its chemically modified derivatives are environmentally ecotoxicological hotpots in recent years, but their toxicity and underlying mechanisms have not been fully identified. Here we employed Caenorhabditis elegans as an animal model to systematically compare the toxicity between nanopolystyrene and its 4 chemically modified derivatives (PS-PEG, PS-COOH, PS-SOOOH and PS-NH) at predicted environmental concentrations. Our study demonstrated that compared with PS exposed group, PS-NH exposure (15 μg/L) caused a significant decline in lifespan by suppressed DAF-16/insulin signaling and shortened body length by inhibiting DBL-1/TGF β signaling. Different from PS-NH exposed group, PS-SOOOH exposure (15 μg/L) could not cause changes in lifespan, but shortened body length by inhibiting DBL-1/TGF β signaling. In addition, PS-COOH, PS-SOOOH or PS-NH exposure (1 μg/L or 15 μg/L) caused more serious toxicity in reducing locomotion behavior and causing gut barrier deficit. Hence the rank order in toxicity of PS-NH>PS-SOOOH>PS-COOH>PS>PS-PEG was identified. Furthermore, we also presented evidence to support the contention that the observed toxic effects on nematodes were linked to oxide stress and activation of anti-oxidative molecules for reversing the adverse effects induced by nanopolystyrene and its 4 chemically modified derivatives. Our data highlighted nanoplastics may be charge-dependently toxic to environmental organisms, and the screened low toxic modification may support polystyrene nanoparticles continued application for daily consumer goods and biomedicine.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135498DOI Listing
June 2022

Natural versus anthropogenic controls on the dissolved organic matter chemistry in lakes across China: Insights from optical and molecular level analyses.

Water Res 2022 Jun 18;221:118779. Epub 2022 Jun 18.

Department of Ocean Science and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. Electronic address:

Dissolved organic matter (DOM) plays an essential role in the global carbon biogeochemical cycle for aquatic ecosystems. The complexity of DOM compounds contributes to the accurate monitoring of its sources and compositions from large-scale patterns to microscopic molecular groups. Here, this study demonstrates the diverse sources and compositions for humic-rich lakes and protein-rich lakes for large-scale regions across China with the linkage to optical components and molecular high-resolution mass spectrometry properties. The total fluorescence intensity of colored DOM (CDOM) for humic-rich lake regions (0.176 Raman unit; R.U.) is significantly (p<0.05) higher than that of the protein-rich lake region (0.084 R.U.). The combined percentages of CDOM absorption variance explained by the anthropogenic and climatic variables across the five lake regions of Northeastern lake region (NLR), Yungui Plateau lake region (YGR), Inner Mongolia-Xinjiang lake region (MXR), Eastern lake region (ELR), and Tibetan-Qinghai Plateau lake region (TQR) were 86.25%, 82.57%, 80.23%, 88.55%, and 87.72% respectively. The averaged relative intensity percentages of CHOS and CHONS formulas from humic-rich lakes (90.831‰, 10.561‰) were significantly higher than that from the protein-like lakes (47.484‰, 5.638‰), respectively. The more complex molecular composition with higher aromaticity occurred in the humic-rich lakes than in the protein-rich lakes. The increasing anthropogenic effects would significantly enhance the sources, transformation, and biodegradation of terrestrial DOM and link to the greenhouse gas emission and the carbon cycle in inland waters.
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http://dx.doi.org/10.1016/j.watres.2022.118779DOI Listing
June 2022

SOX9 and IL1A as the potential gene biomarkers of the oral cancer.

Comb Chem High Throughput Screen 2022 Jun 28. Epub 2022 Jun 28.

Department of Stomatology, The Fourth Hospital of Hebei Medical University. No. 12, Jiankang Road, Chang\'an District, Shijiazhuang City, Hebei, China.

Objective: Oral cancer is one of the most common malignant tumors in the head and neck. It is easy to relapse and the prognosis is poor. However, the molecular mechanism in the development of oral cancer is still unclear.

Methods: A total of 30 normal individuals and 30 patients with head and neck cancer who underwent surgery were recruited in the Fourth Hospital of Hebei Medical University between February 2019 and November 2021. And Human Protein Atlas (HPA) analysis, real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence were used to verify the expression of SRY-Box Transcription Factor 9 (SOX9) and interleukin 1 A (IL1A). The GSE69002 dataset was downloaded from the gene expression omnibus (GEO) database. GEO2R was used to identify the differently expressed genes (DEGs). The protein-protein interaction (PPI) network was constructed by using the search tool for the retrieval of interacting genes/proteins (STRING), and Cytoscape software was performed for visualization. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) for enrichment analysis was made via the Database for Annotation Visualization and Integrated Discovery (DAVID), Metascape, Gene Set Enrichment Analysis (GSEA), and Bin Gene Ontology (BINGO) analysis. Gene Expression Profiling Interactive Analysis (GEPIA) analysis was used to analyze the expression level of hub genes and pathological stage. The cBioPortal can be used for mutation analysis and pathway prediction of hub genes. Kaplan Meier Plotter was used for survival analysis of hub genes.

Results: The relative expression level of SOX9 (P=0.021, t=4.332) and IL1A (P=0.011, t= -4.213) in oral cancer was significantly higher than that in normal group. The DEGs mainly enriched in the cell division, inflammation, interleukin-12 beta subunit binding, interleukin-10 receptor binding. All the differentially expressed gene pathways eventually converge in cell growth and apoptosis. No relationships between the pathologic stage and expression of hub genes. The poor overall survival of patients with the high expression of SOX9 [hazard ratio (HR) = 1.46, P = 0.009] and IL1A (HR = 1.49, P = 0.008); There were strong correlations between the hub genes and the head and neck neoplasms via the comparative toxicogenomics database (CTD). The PCR results showed that the level of SOX9 (P<0.001, t = -23.368) in the cancer group was significantly higher than that in the normal group; The level of IL1A in cancer group was significantly higher than that in normal group (P<0.001, t = -11.960). Furthermore, the expression levels of SOX9 and IL1A were verified by the immunofluorescence assay.

Conclusion: SOX9 and IL1A genes are highly expressed in oral cancer and might be potential therapeutic targets for oral cancer. The poor overall survival of patients with the high expression of SOX9 and IL1A.
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http://dx.doi.org/10.2174/1386207325666220628091041DOI Listing
June 2022

Twin-Field Quantum Digital Signature with Fully Discrete Phase Randomization.

Entropy (Basel) 2022 Jun 18;24(6). Epub 2022 Jun 18.

School of Information Science and Technology, Northwest University, Xi'an 710127, China.

Quantum digital signatures (QDS) are able to verify the authenticity and integrity of a message in modern communication. However, the current QDS protocols are restricted by the fundamental rate-loss bound and the secure signature distance cannot be further improved. We propose a twin-field quantum digital signature (TF-QDS) protocol with fully discrete phase randomization and investigate its performance under the two-intensity decoy-state setting. For better performance, we optimize intensities of the signal state and the decoy state for each given distance. Numerical simulation results show that our TF-QDS with as few as six discrete random phases can give a higher signature rate and a longer secure transmission distance compared with current quantum digital signatures (QDSs), such as BB84-QDS and measurement-device-independent QDS (MDI-QDS). Moreover, we provide a clear comparison among some possible TF-QDSs constructed by different twin-field key generation protocols (TF-KGPs) and find that the proposed TF-QDS exhibits the best performance. Conclusively, the advantages of the proposed TF-QDS protocol in signature rate and secure transmission distance are mainly due to the single-photon interference applied in the measurement module and precise matching of discrete phases. Besides, our TF-QDS shows the feasibility of experimental implementation with current devices in practical QDS system.
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http://dx.doi.org/10.3390/e24060839DOI Listing
June 2022

How do Paraspinal Muscles Contract during the Schroth Exercise Treatment in Patients with Adolescent Idiopathic Scoliosis (AIS)?

Bioengineering (Basel) 2022 May 27;9(6). Epub 2022 May 27.

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong SAR 999077, China.

The Schroth exercise can train the paraspinal muscles of patients with adolescent idiopathic scoliosis (AIS), however, muscle performance during the training remains unknown. This study applied surface electromyography (sEMG) to investigate the paraspinal muscle activities before, during and after Schroth exercise in nine AIS patients. This study found that after the Schroth exercise, the paraspinal muscle symmetry index (PMSI) was significantly reduced (PMSI = 1.3), while symmetry exercise significantly lowered the PMSI (PMSI = 0.93 and 0.75), and asymmetric exercise significantly increased the PMSI (PMSI = 2.56 and 1.52) compared to relax standing (PMSI = 1.36) in participants ( < 0.05). Among the four exercises, the PMSI of on all fours (exercise 1) and kneeling on one side (exercise 3) was the most and the least close to 1, respectively. The highest root mean square (RMS) of sEMG at the concave and convex side was observed in squatting on the bar (exercise 2) and sitting with side bending (exercise 4), respectively. This study observed that the asymmetric and symmetric exercise induced more sEMG activity on the convex and concave side, respectively, and weight bearing exercise activated more paraspinal muscle contractions on both sides of the scoliotic curve in the included AIS patients. A larger patient sample size needs to be investigated in the future to validate the current observations.
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http://dx.doi.org/10.3390/bioengineering9060234DOI Listing
May 2022

The Predictive Role of Aberrant Metabolic Parameters and Negative Automatic Thinking on the Cognitive Impairments Among Schizophrenia Patients with Metabolic Syndrome.

Neuropsychiatr Dis Treat 2022 7;18:1087-1097. Epub 2022 Jun 7.

Affiliated Psychological Hospital of Anhui Medical University, Hefei, People's Republic of China.

Purpose: The study aimed to clarify the cognitive impairments of schizophrenia with metabolic syndrome while evaluating their potential as risk factors.

Patients And Methods: We recruited 153 participants and divided them into three groups according to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, criteria and the guideline standards for the prevention and treatment of dyslipidemia in Chinese adults in 2007 for metabolic syndrome, as follows: healthy control group (n = 47); nonmetabolic syndrome group (n = 58); and metabolic syndrome group (n = 48). Psychotic symptoms were evaluated using the Positive and Negative Syndrome Scale. Cognitive function and automatic thinking were estimated using the Montreal Cognitive Assessment Scale, Verbal Fluency Test, and Automatic Thoughts Questionnaire. Serum biochemical parameters were measured by automatic biochemistry analyzer.

Results: One-way ANOVA analysis revealed that differential cognition impairments in schizophrenia patients compared to controls. Furthermore, results of multiple comparisons showed that more serious barriers in orientation, language fluency, and negative automatic thinking existed in the metabolic syndrome group than in the healthy and non-metabolic syndrome groups. Spearman correlation and stepwise linear regression analyses showed that psychopathological symptoms, high waist circumference, and high triglyceride were the predictive factors for negative automatic thoughts, orientation, and language fluency. Those results collectively revealed that high waist circumference, high triglyceride and negative automatic thinking had validity and effectiveness in predicting the cognitive function impairments of the metabolic syndrome group.

Conclusion: The present findings strongly supported the notion that aberrant parameters of high waist circumference, high triglyceride and high negative automatic thoughts had validity and effectiveness predictive role for cognitive impairments in the schizophrenics with metabolic syndrome. The schizophrenia patients with metabolic syndrome should receive regular monitoring and adequate treatment for metabolic and psychological risk factors.
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http://dx.doi.org/10.2147/NDT.S367392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188464PMC
June 2022

Olive oil classification with Laser-induced fluorescence (LIF) spectra using 1-dimensional convolutional neural network and dual convolution structure model.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 21;279:121418. Epub 2022 May 21.

Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China. Electronic address:

Laser-induced fluorescence (LIF) spectroscopy is widely used for the analysis and classification of olive oil. This paper proposes the classification of LIF data using a specific 1-dimensional convolutional neural network (1D-CNN) model, which does not require pre-processing steps such as normalisation or denoising and can be flexibly applied to massive data. However, by adding a dual convolution structure (Dual-conv) to the model, the features of the 1-dimensional spectra are more scattered within one convolution-pooling process; thus, the classification effects are improved. The models were validated through an olive oil classification experiment which contained a total of 72,000 sets of LIF spectra data, and the classification accuracy rate reached ∼99.69%. Additionally, a common classification approach, the support vector machine (SVM), was utilised for the comparison of the results. The results show that the neural networks perform better than the SVM. The Dual-conv model structure has a faster convergence speed and higher evaluation parameters than those of the 1D-CNN in the same period of iterations, without increasing the data dimension.
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http://dx.doi.org/10.1016/j.saa.2022.121418DOI Listing
October 2022

Application Effects of SBAR Communication Mode in ICU Nursing Physical Restraint Shift.

Altern Ther Health Med 2022 Jun 10. Epub 2022 Jun 10.

Context: Correct and effective handovers of patients' information during shift changes can ensure patients' safety and can help an incoming shift of nurses to continuously monitor patients' psychological problems and avoid unnecessary physical restraints. Development of a standard procedure for handover of patients who have been physically restrained has important clinical significance related to the smooth continuation of nursing work and assurance of the quality of care.

Objective: The study intended to investigate the clinical effects of the situation-background-assessment-recommendation (SBAR) communication mode on the quality of the information passed during shift changes about patients in intensive care units (ICUs) who had been physically restrained and to compare it to the clinical effects obtained using traditional methods of communication.

Design: The study was a retrospective analysis of the process used by nurses who were passing patient's information during shift changes when caring for patients who had been physically restrained.

Setting: The study took place in an ICU at the Second Hospital of Hebei Medical University in Shijiazhuang, Hebei, China.

Participants: Participants were 21 nurses caring for 239 ICU patients under physical restraint at the Second Hospital.

Intervention: Of the 239 patients, 118 had been hospitalized between March 1 and March 15, 2018 and were assigned to the control group, and 121 had been hospitalized between June 1 and June 15, 2018 and were assigned to the intervention group. An ICU Physical Restraint Handover Order was established according to the SBAR communication mode. The intervention group used the SBAR communication mode and the control group used the hospital's routine communication mode for the physical restraint of a patient during a nursing shift.

Outcome Measures: The study measured the differences between the groups in the nurses' passing rates based on standards for the use of physical restraints, the quality of handover of information during shift changes about patients under physical restraint, the quality of the documentation written by nurses about the physical restraint, and the nurses' satisfaction with the handover of information during a shift change.

Results: Among the patient, 112 in the intervention group (92.56%) and 92 in the control group (77.97%) were qualified for physical restraint. A statistically significant difference existed between the two groups in the passing rate for the use of physical restraints (P = .001). The quality score for the handovers during shift changes of patients under physical restraint in the intervention group was 95.46 ± 2.50 and for the control group was 91.08 ± 3.57, with the difference being statistically significant (P = .030). The quality score for the nursing documentation for the intervention group, at 97.21 ± 1.49, was higher than that of the control group, at 90.78 ± 3.42, and the difference was statistically significant (P < .001). The nurses' satisfaction score for the intervention group, at 98.14 ± 1.01 was higher than that of the control group, at 92.57 ± 1.86, and the difference was statistically significant (P = .006).

Conclusions: The use of the SBAR communication mode to improve the information passed to nurses about patients under physical restraint during a shift change can improve the quality of the physical restraint and nurses' satisfaction and has a better clinical-application effect than the traditional methods used during shift changes.
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June 2022

Carbon Sequestration in the Form of Recalcitrant Dissolved Organic Carbon in a Seaweed (Kelp) Farming Environment.

Environ Sci Technol 2022 Jun 10. Epub 2022 Jun 10.

Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

Under climate change scenarios, the contribution of macroalgae to carbon sequestration has attracted wide attention. As primary producers, macroalgae can release substantial amounts of dissolved organic carbon (DOC) in seawater. However, little is known about the molecular composition and chemical properties of DOC derived from macroalgae and which of them are recalcitrant DOC (RDOC) that can be sequestered for a long time in the ocean. In the most intensive seaweed (kelp) farming area (Sanggou Bay) in China, we found that kelp mariculture not only significantly increased DOC concentration, but also introduced a variety of new DOC molecular species, many of which were sulfur-containing molecules. A long-term DOC degradation experiment revealed that those DOC with strong resistance to microbial degradation, i.e., RDOC, account for approximately 58% of the DOC extracted from kelp mariculture area. About 85% (3587 out of 4224 with different chemical features) of the RDOC molecular species were steadily present throughout the long-term degradation process. 15% (637 out of 4224 with different chemical features) of the RDOC molecular species were likely newly generated by microorganisms after metabolizing macroalgae-derived labile DOC. All these stable RDOC should be included in the blue carbon budgets of seaweed.
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http://dx.doi.org/10.1021/acs.est.2c01535DOI Listing
June 2022

Chemical Constituents from the Flowers of L. and Their Lung Protective Activity.

Molecules 2022 Jun 2;27(11). Epub 2022 Jun 2.

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.

A new flavonoid, saffloflavanside (), a new sesquiterpene, safflomegastigside (), and a new amide, saffloamide (), together with twenty-two known compounds (-), were isolated from the flowers of L. Their structures were determined based on interpretation of their spectroscopic data and comparison with those reported in the literature. The protective effects against lipopolysaccharide (LPS)-stimulated damage on human normal lung epithelial (BEAS-2B) cells of the compounds were evaluated using MTT assay and cellular immunofluorescence assay. The results showed that compounds -, -, and - exhibited protective effects against LPS-induced damage to BEAS-2B cells. Moreover, compounds -, -, and - can significantly downregulate the level of nuclear translocation of NF-κB p-p65. In summary, this study revealed chemical constituents with lung protective activity from , which may be developed as a drug for the treatment of lung injury.
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http://dx.doi.org/10.3390/molecules27113573DOI Listing
June 2022

Exploring the link between cognitive deficit, self-esteem, alexithymia, and depressive symptom of schizophrenia.

Brain Behav 2022 Jun 8:e2648. Epub 2022 Jun 8.

Affiliated Psychological Hospital of Anhui Medical University, Hefei, Anhui, China.

Objective: To the best of our knowledge, studies have been rarely conducted to assess the correlation between cognitive deficit, self-esteem, and alexithymia in the depressive symptoms of schizophrenia (SCZ). Therefore, this study aims to explore the risk factors associated with impairment of cognitive function, alexithymia, and self-esteem among a representative sample of first-episode schizophrenic patients.

Method: We recruited 107 first-episode schizophrenic patients (48.6% male, 51.4% female, 36.94 ± 10.73 years) into the research group, according to the Diagnostic and Statistical Manual of Mental Disorders (5th edition). A total of 45 healthy people (51.1% male, 48.9% female, 32.47 ± 10.94 years) were enlisted in the healthy control group. Psychotic symptoms were evaluated using the Positive and Negative Syndrome Scale (PANSS). Cognitive functions were estimated using the Montreal Cognitive Assessment Scale (MoCA). The feelings of respect and self-acceptance were tested using the Rosenberg Self-Esteem Scale (RSES). Emotion of identifying and describing were measured by self-report scale of Toronto Alexithymia Scale-20 (TAS-20).

Results: Overall cognitive impairment and alexithymia were found more serious in the patients of SCZ than the healthy group (p < .001, respectively). The patients of SCZ have higher self-esteem than the healthy group (p = .013). Total score of MoCA, ability of visual space and executive function, and delayed recall were explored had negatively correlation with alexithymia (r = -.319, p = .001; r = -.248, p = .010; r = -0.263, p = .006). Total score of RSES and depressive symptoms of PANSS had a positive correlation with alexithymia (r = .394, p = .001; r = .208, p = .032). Stepwise regression analyses have shown a positive relationship between difficulty describing feelings and depression subscale of PANSS (β = .188, t = -2.007, p = .047) while a negative relationship between externally oriented thinking and depression subscale of PANSS (β = -.244, t = -2.603, p = .011). A positive link correlation also was found between the total scores of TAS and RSES (β = .372, t = 4.144, p = .001). A negative relevance was found between the total scores of TAS and scores of MoCA (β = -.305, t = -3.348, p = .001).

Conclusion: Overall impairment of cognitive function and alexithymia are commonly encountered in SCZ patients. Poor cognitive function, alexithymia, and high level self-esteem may be specific detective risk factors for the depressive symptoms of SCZ.
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http://dx.doi.org/10.1002/brb3.2648DOI Listing
June 2022

Hyperuricemia is Associated With 2- and 5-Year Adverse Outcomes in Patients With ST-Segment Elevation Myocardial Infarction Undergoing Percutaneous Coronary Intervention.

Front Endocrinol (Lausanne) 2022 19;13:852247. Epub 2022 May 19.

Department of Cardiology, Centre for Coronary Heart Disease, National Clinical Research Center for Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Hyperuricemia has recently been identified as a risk factor of cardiovascular diseases; however, prognostic value of hyperuricemia in patients with ST-segment elevation myocardial infarction (STEMI) remained unclear. Simultaneously, the mechanism of this possible relationship has not been clarified. At present, some views believe that hyperuricemia may be related to the inflammatory response. Our study aimed to investigate the association between hyperuricemia and long-term poor prognosis and inflammation in STEMI patients undergoing percutaneous coronary intervention (PCI).

Methods: A total of 1,448 consecutive patients with STEMI were studied throughout 2013 at a single center. The primary endpoint was all-cause death at 2- and 5-year follow-up. Inflammatory biomarkers were collected on admission of those patients: high sensitive C-reactive protein (hs-CRP), erythrocyte sedimentation rate (ESR), and white blood cell (WBC) count.

Results: Hyperuricemia was associated with higher 2- and 5-year all-cause death in STEME patients compared to normouricemia (5.5% vs. 1.4%, P <0.001; 8.0% vs 3.9%, P = 0.004; respectively). After multivariable adjustment, hyperuricemia was still an independent predictor of 2-year all-cause death (hazard ratio (HR) =4.332, 95% confidence interval (CI): 1.990-9.430, P <0.001) and 5-year all-cause death (HR =2.063, 95% CI: 1.186-3.590, P =0.010). However, there was no difference in hs-CRP, ESR, and WBC count on admission in STEMI patients with hyperuricemia compared to normouricemia (P >0.05).

Conclusions: Hyperuricemia was associated with higher risks of 2- and 5-year all-cause deaths in patients with STEMI undergoing PCI. However, this study did not find a correlation between hyperuricemia and inflammatory responses in newly admitted STEMI patients.
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http://dx.doi.org/10.3389/fendo.2022.852247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160184PMC
June 2022

Oxygen availability driven trends in DOM molecular composition and reactivity in a seasonally stratified fjord.

Water Res 2022 Jul 29;220:118690. Epub 2022 May 29.

Joint Laboratory for Ocean Research and Education at Dalhousie University, Shandong University and Xiamen University, Halifax, Canada, Qingdao, China and Xiamen, China; State Key Laboratory for Marine Environmental Science and College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

Ocean deoxygenation could potentially trigger substantial changes in the composition and reactivity of dissolved organic matter (DOM) pool, which plays an important role in the global carbon cycle. To evaluate links between DOM dynamics and oxygen availability, we investigated the DOM composition under varying levels of oxygen in a seasonally hypoxic fjord through a monthly time-series over two years. We used ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) to characterize DOM on a molecular level. We find a clear trend both in diversity and molecular composition of the DOM along the oxygen gradient. As oxygen decreased, the chemodiversity was significantly increased, along with accumulation of relatively high-molecular-weight, reduced and unsaturated compounds enriched with carboxyl-group structures, which were also thermodynamically less favorable to biodegradation. Our results suggested that oxygen depletion selectively protected otherwise bioavailable compounds from decomposition and may promote the accumulation of a larger recalcitrant DOM pool in the global ocean, which could provide negative feedback to the ocean carbon sequestration and climate change.
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http://dx.doi.org/10.1016/j.watres.2022.118690DOI Listing
July 2022

Molecular insights into the transformation of refractory organic matter in landfill leachate nanofiltration concentrates during a flocculation and O/HO treatment.

J Hazard Mater 2022 08 20;435:128973. Epub 2022 Apr 20.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, 611756, China. Electronic address:

During leachate treatment, molecular information regarding the completely removed, partially removed, less-reactive, increased, and produced parts of dissolved organic matter (DOM) remains unknown. This study applied ESI FT-ICR MS to investigate the transformation characteristics of leachate nanofiltration concentrate (NFC) DOM during a combined flocculation-O/HO process. The NFC contained 5069 compounds in four main classes (CHO, CHON, CHOS, and CHONS compounds). The DOM number decreased to 4489 during flocculation and to 2903 after the O/HO process. During flocculation, the completely and partially removed DOM was mainly low-oxygen unsaturated and phenolic compounds. Saturated DOM was produced and remained in the flocculated effluent. During the O/HO process, the completely and partially removed DOM were mainly low-oxygen unsaturated and phenolic compounds that were mainly in a reduced state. Flocculation can remove many (condensed) aromatic compounds, and methylation and hydrogenation reactions occurred during flocculation. In the O/HO process, dearomatization, demethylation, carboxylation, and carbonylation reactions further achieved the degradation of DOM that was resistant to flocculation. Overall, the combined flocculation-O/HO process collectively eliminated a broader range of DOM than the single processes could achieve. The results of this study provide an in-depth understanding of DOM transformation in an NFC treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128973DOI Listing
August 2022

Non-Invasive Liver Fibrosis Scores Are Associated With Contrast-Associated Acute Kidney Injury in Patients Undergoing Elective Percutaneous Coronary Intervention.

Angiology 2022 May 31:33197221105745. Epub 2022 May 31.

Department of Cardiology, 117861Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, China.

Previous studies have demonstrated that non-invasive liver fibrosis scores (LFSs) are associated with kidney function deterioration. This study aimed to assess the predictive performance of LFSs in contrast-associated acute kidney injury (CA-AKI) in coronary artery disease (CAD) patients undergoing elective percutaneous coronary intervention (PCI). This retrospective study involved 5627 patients. The frequency of CA-AKI was 6.3% (n = 353). In a multivariate logistic analysis after adjustment, non-invasive LFSs, including fibrosis-5 score (FIB-5), fibrosis-4 score (FIB-4), aspartate aminotransferase to alanine aminotransferase ratio (AAR), and aspartate aminotransferase to platelet ratio index were independent risk factors for CA-AKI (all < .05), whereas the Forns score was not ( > .05). The highest predictive performance was observed for FIB-5 (area under the curve [AUC] = .644) compared to other LFSs. A restricted cubic spline analysis confirmed approximately linear relationships between LFSs and risks of CA-AKI. Furthermore, adding FIB-5 (AUC = .747; net reclassification improvement [NRI] = .441, < .001; integrated discrimination improvement [IDI] = .008, < .001) or AAR (AUC = .747; NRI = .419, < .001; IDI = .006, = .010) to an established clinical risk model could significantly improve the prediction of CA-AKI. The LFSs were significantly associated with CA-AKI, possibly serving as predictive tools for early identification of CAD patients undergoing elective PCI that are at high risk of CA-AKI.
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http://dx.doi.org/10.1177/00033197221105745DOI Listing
May 2022

PLAG1 dampens protein synthesis to promote human hematopoietic stem cell self-renewal.

Blood 2022 May 31. Epub 2022 May 31.

McMaster University.

Hematopoietic stem cell (HSC) dormancy is understood as supportive of HSC function and their long-term integrity. While regulation of stress responses incurred as a result of HSC activation is recognized as important in maintaining stem cell function, little is understood of the preventative machinery present in human HSCs that may serve to resist their activation and promote HSC self-renewal. We demonstrate that the transcription factor PLAG1 is essential for long-term HSC function and when overexpressed endows a 15.6-fold enhancement in the frequency of functional HSC in stimulatory conditions. Genome-wide measures of chromatin occupancy and PLAG1-directed gene expression changes combined with functional measures reveal that PLAG1 dampens protein synthesis, restrains cell growth and division, and enhances survival, with the primitive cell advantages it imparts being attenuated by addition of the potent translation activator, c-MYC. We find PLAG1 capitalizes on multiple regulatory factors to ensure protective diminished protein synthesis including 4EBP1 and translation-targeting miR-127, and does so independently of stress response signaling. Overall, our study identifies PLAG1 as an enforcer of human HSC dormancy and self-renewal through its highly context-specific regulation of protein biosynthesis, and classifies PLAG1 among a rare set of bona fide regulators of mRNA translation in these cells. Our findings showcase the importance of regulated translation control underlying human HSC physiology, its dysregulation under activating demands, and the potential if its targeting for therapeutic benefit.
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http://dx.doi.org/10.1182/blood.2021014698DOI Listing
May 2022

An ASIP for Neural Network Inference on Embedded Devices with 99% PE Utilization and 100% Memory Hidden under Low Silicon Cost.

Sensors (Basel) 2022 May 19;22(10). Epub 2022 May 19.

School of Information and Electronics, Beijing Institute of Technology, Beijing 100811, China.

The computation efficiency and flexibility of the accelerator hinder deep neural network (DNN) implementation in embedded applications. Although there are many publications on deep neural network (DNN) processors, there is still much room for deep optimization to further improve results. Multiple dimensions must be simultaneously considered when designing a DNN processor to reach the performance limit of the architecture, including architecture decision, flexibility, energy efficiency, and silicon cost minimization. Flexibility is defined as the ability to support as many multiple networks as possible and to easily adjust the scale. For energy efficiency, there are huge opportunities for power efficiency optimization, which involves access minimization and memory latency minimization based on on-chip memory minimization. Therefore, this work focused on low-power and low-latency data access with minimized silicon cost. This research was implemented based on an ASIP (application specific instruction set processor) in which an ISA was based on the caffe2 inference operator and the hardware design was based on a single instruction multiple data (SIMD) architecture. The scalability and system performance of our SoC extension scheme were demonstrated. The VLIW was used to execute multiple instructions in parallel. All costs for data access time were thus eliminated for the convolution layer. Finally, the processor was synthesized based on TSMC 65 nm technology with a 200 MHz clock, and the Soc extension scheme was analyzed in an experimental model. Our design was tested on several typical neural networks, achieving 196 GOPS at 200 MHz and 241 GOPS/W on the VGG16Net and AlexNet.
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http://dx.doi.org/10.3390/s22103841DOI Listing
May 2022

New Evidence of Central Nervous System Damage in Diabetes Mellitus: Impairment of Fine Visual Discrimination.

Diabetes 2022 May 25. Epub 2022 May 25.

Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.

Diabetes can damage both the peripheral sensory organs, causing retinopathy, as well as the central visual system, leading to contrast sensitivity and impaired color vision in patients without retinopathy. Orientation discrimination is important for shape recognition by the visual system. Our psychophysical findings in this study show diminished orientation discrimination in diabetic patients without retinopathy. To reveal the underlying mechanism, we established a diabetic mouse model and recorded in vivo electrophysiological data in the dorsal lateral geniculate nucleus (dLGN) and primary visual cortex (V1). Reduced orientation selectivity was observed in both individual and populations of neurons in V1 and dLGN, which increased in severity with disease duration. This diabetes-associated neuronal dysfunction appeared earlier in the V1 than dLGN. Additionally, neuronal activity and signal-to-noise ratio is reduced in V1 neurons of diabetic mice, leading to a decreased capacity for information processing by V1 neurons. Notably, the V1 in diabetic mice exhibits reduced excitatory neuronal activity and lower levels of phosphorylated mammalian target of rapamycin (mTOR). Our findings show that altered responses of both populations and single V1 neurons may impair fine vision, thus expanding our understanding of the underlying causes of diabetes-related impairment of the central nervous system.
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http://dx.doi.org/10.2337/db21-0715DOI Listing
May 2022

miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing.

Cell Death Dis 2022 May 25;13(5):494. Epub 2022 May 25.

Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, 410008, Hunan, China.

A specific bone capillary subtype, namely type H vessels, with high expression of CD31 and endomucin, was shown to couple angiogenesis and osteogenesis recently. The number of type H vessels in bone tissue declines with age, and the underlying mechanism for this reduction is unclear. Here, we report that microRNA-188-3p (miR-188-3p) involves this process. miRNA-188-3p expression is upregulated in skeletal endothelium and negatively regulates the formation of type H vessels during ageing. Mice with depletion of miR-188 showed an alleviated age-related decline in type H vessels. In contrast, endothelial-specific overexpression of miR-188-3p reduced the number of type H vessels, leading to decreased bone mass and delayed bone regeneration. Mechanistically, we found that miR-188 inhibits type H vessel formation by directly targeting integrin β3 in endothelial cells. Our findings indicate that miR-188-3p is a key regulator of type H vessel formation and may be a potential therapeutic target for preventing bone loss and accelerating bone regeneration.
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http://dx.doi.org/10.1038/s41419-022-04902-wDOI Listing
May 2022

Design and characterization of third-order Sallen-Key digital filter in nuclear signal processing.

Appl Radiat Isot 2022 May 20;186:110277. Epub 2022 May 20.

School of Nuclear Science and Engineering, East China University of Technology, Nanchang, 330013, China.

The Gaussian filter shaping circuit is widely used in the nuclear pulse signal processing due to its good performance in amplitude extraction and pulse counting. A third-order Sallen-Key (3rd S-K) filter shaping circuit is designed based on a RC integrator and a second-order Sallen-Key (2nd S-K) circuit. According to the digital 3rd S-K, the transfer functions is derived in the Laplacian domain, and the numerical recurrence model is analyzed and researched, the purpose is to obtain its transfer function and amplitude-frequency response curve in the z-domain. For the simulation and actual sampling of the nuclear signal, digital shaping processing is performed at different parameters, three parameters (d, SNR, δ) are defined to compare and analyze the amplitude extraction, noise suppression and symmetry of the digital shaping method, which shows that as the shaping parameters increases, the digital shaping output noise suppression performance is better, the SNR increased from 49.25 to 64.21, the waveform is more symmetrical, the δ reduced from 34.05 to 0.22. At the same parameters, it is compared and analyzed with CR-RC and 2nd S-K shaping methods, according to the digital Gaussian shaping results, the 3rd S-K digital shaping method has better pulse amplitude extraction(d = 36.06%), noise suppression performance (SNR = 64.21) and waveform symmetry (δ = 0.22). Under different shaping methods, the energy resolution and pulse counting rate of the Fe characteristic X-ray energy spectrum are compared based on a Si-PIN detector. The results show that the 3rd S-K digital shaping method has better energy resolution performance and comprehensive performance indicators, which can be further applied for digital shaping of nuclear pulse signals.
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http://dx.doi.org/10.1016/j.apradiso.2022.110277DOI Listing
May 2022

Recent Advances in Carbon Dots for Fluorescent Bioimaging: A Mini-Review.

Front Chem 2022 5;10:905475. Epub 2022 May 5.

Department of Pharmacy, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.

As a new type of "zero-dimensional" fluorescent carbon nanomaterials, carbon dots (CDs) have some unique optical and chemical properties, they are being explored for a variety of applications in bio-related fields, such as bioimaging, biosensors, and therapy. This review mainly summarizes the recent progress of CDs in bioimaging. The overview of this review can be roughly divided into two categories: (1) bioimaging based on CDs in different cells and important organelles. (2) The distribution, imaging and application of CDs in mice and zebrafish. In addition, this review also points out the potential advantages and future development directions of CDs for bioimaging, which may promote the development of CDs in the field of bioimaging.
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http://dx.doi.org/10.3389/fchem.2022.905475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117726PMC
May 2022

Ten undescribed diterpenoid quinones derived from the Salvia miltiorrhiza.

Phytochemistry 2022 Aug 16;200:113224. Epub 2022 May 16.

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, 450046, PR China. Electronic address:

This study aimed to report the structure elucidation of the compounds isolated from Salvia miltiorrhiza, and their biological evaluations. Ten undescribed diterpenoid quinones and 10 known analogues were isolated from the dried roots of S. miltiorrhiza. Their structures were elucidated by extensive analysis, including nuclear magnetic resonance, high-resolution mass spectra, and ultraviolet and infrared spectra. Their absolute configurations were determined by comparing the experimental and calculated electronic circular dichroism spectra. In the evaluation of bioactivities, Salvianolactone acid I, epi-danshenspiroketallactone F, danshinspiroketallactone, grandifolia G, and 2H-Naphtho [1,8-bc]furan (10 μM) significantly increased cell viability and decreased the nuclear transport of p-P65 in lipopolysaccharide-induced bronchial epithelial cells. It was concluded that the diterpenoid quinones might belong to potent targeted lung-protective agents.
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http://dx.doi.org/10.1016/j.phytochem.2022.113224DOI Listing
August 2022

Temperature Rise Increases the Bioavailability of Marine -Derived Dissolved Organic Matter.

Front Microbiol 2022 19;13:838707. Epub 2022 Apr 19.

Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

is one group of main primary producers and plays a key role in oceanic carbon fixation and transformation. To explore how the temperature rise affects the bioavailability of -derived dissolved organic matter (SOM) and whether this effect would be altered by the involvement of heterotrophic bacteria, we compared the optical and molecular properties of the SOM of axenic sp. PCC7002 culture () to that with associated heterotrophic bacteria (B) under 15, 18, and 21C growth temperatures at exponential and decay growth phases. Our results showed that the temperature rise increased the bioavailability of the SOM of both and B cultures by lowering the proportion of the hydrogen-poor and double-bond structure-rich humus-like components and highly unsaturated substances, as indicated by the increase of spectral slope ratio (S ) and biological index (BIX) and decrease of humification index (HIX). Moreover, the involvement of heterotrophic bacteria modified the -derived SOM, together with its intracellular dissolved organic matter (DOM) excludes, lowering the SOM bioavailability. Our results indicated that the warming in climate change scenario may enhance the bioavailability of the -derived SOM although it may be tempered by the involvement of heterotrophic bacteria, providing an insight for preservation of the organic carbon pool in global oceans.
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http://dx.doi.org/10.3389/fmicb.2022.838707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097602PMC
April 2022

Separation and characterization of sulfonates in dissolved organic matter from industrial wastewater by solid phase extraction and high-resolution mass spectrometry.

Anal Bioanal Chem 2022 Jul 12;414(16):4697-4706. Epub 2022 May 12.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.

Electrospray ionization (ESI) source combined with high-resolution mass spectrometry (HRMS) has been successfully used to characterize the molecular composition of dissolved organic matter (DOM) in industrial wastewaters. However, sulfonates are commonly presenting in these wastewaters and showing strong ionization suppression for the ESI analysis, which seriously affects the characterization of other DOM components. In this paper, a novel method based on polymer anion exchange (PAX) solid phase extraction (SPE) was proposed to separate DOMs from petroleum refining and coal coking wastewaters into hydrophobic neutral, hydrophobic acids, and hydrophobic strong acids; the fractions were analyzed by negative-ion ESI Fourier transform ion cyclotron resonance mass spectrometry. The results show that PAX-SPE separated sulfonates from wastewaters and enabled the molecular characterization of oxygen-containing compounds, which are the major component of DOM in the wastewaters. Semi-quantitative analysis of sulfonates in the wastewaters was carried out and the results show that these compounds account for 11% and 2% in total organic carbon for the refinery and coal coking wastewaters, respectively. PAX-SPE with stepwise elution and followed by high-resolution mass spectrometry analysis enables a comprehensive characterization for the molecular composition of industrial wastewater DOM. The method has potential for extensive application and the results are instructive for the understanding of the molecular composition of DOM in industrial wastewaters.
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http://dx.doi.org/10.1007/s00216-022-04092-6DOI Listing
July 2022

New Approach to Generate Ratiometric Signals on Immunochromatographic Strips for Small Molecules.

Anal Chem 2022 05 10;94(20):7358-7367. Epub 2022 May 10.

College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

The self-calibration capability of ratiometric signals has been widely considered to enhance the accuracy, sensitivity, and anti-interference ability of immunoassays. Exploring a new approach to generate ratiometric signals can provide more options for various requirements. Herein, we integrated the negative-readout competitive and positive-readout noncompetitive immunoassays into a single assay by employing different color tracers, labeled peptidomimetic and anti-immunocomplex peptides, to create a new unconstrained ratiometric signal approach. Using an immunochromatographic strip (ICS) and a fungicide benzothiostrobin as the analytical platform and analyte, respectively, we showed that this approach can be extensively applied to fluorescence and colorimetry readouts, which have also been proven for strong anti-interference ability to an external light environment. Moreover, the enormous intuitional color changes of ratiometric fluorescent and colorimetric ICSs (RFICS and RCICS) enabled the formation of the color reference cards (like the pH paper) for visual judgment. After adaptation with a portable smartphone, the quantitative detection limits for RFICS and RCICS were 0.17 and 0.44 ng mL, respectively. In addition, the ICSs showed good accuracy for the detection of benzothiostrobin in spiked samples.
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http://dx.doi.org/10.1021/acs.analchem.2c00838DOI Listing
May 2022

Growth arrest-specific transcript 5 represses endometrial cancer development by promoting antitumor function of tumor-associated macrophages.

Cancer Sci 2022 May 9. Epub 2022 May 9.

Department of Gynecology, Shenzhen Second People's Hospital/The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

The tumor-suppressor role of long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) has been proven in various types of cancer. However, the specific function of GAS5 in tumor-associated macrophages (TAMs) of endometrial cancer (EC) is elusive. Quantitative PCR results showed that GAS5 expression decreased in EC tissues and primary TAMs from EC tumors. Tumor-associated macrophage infiltration was significantly positively associated with the developmental stage of EC. Direct coculture of GAS5-overexpressing TAMs and EC cells showed that GAS5 enhanced phagocytosis, antigen presentation, and activation of cytotoxic T cells, and repressed "Don't eat me" signals between TAMs and EC cells. Tumor formation in immunodeficient mice showed that GAS5-overexpressing macrophages could repress EC formation in vivo. GAS5 promoted M1 polarization by activating the microRNA-21- phosphatase and tensin homolog (PTEN)-AKT signaling pathway and directly repressing the nuclear accumulation and phosphorylation of oncogenic yes-associated protein 1 (YAP1) in TAMs. GAS5 inhibited the development of EC from both innate and adaptive immunity by transforming TAMs from a protumor to an antitumor phenotype. These antitumor effects of GAS5 on TAMs were mediated by the activation of the miR-21-PTEN-AKT pathway and inhibition of YAP1.
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http://dx.doi.org/10.1111/cas.15390DOI Listing
May 2022

Effects of mental health interventions among people hospitalized with COVID-19 infection: A systematic review of randomized controlled trials.

Gen Hosp Psychiatry 2022 Jul-Aug;77:40-68. Epub 2022 Apr 9.

Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada; Department of Psychiatry, McGill University, Montreal, Quebec, Canada; Department of Psychology, McGill University, Montreal, Quebec, Canada; Department of Epidemiology, Biostatistics, and Occupational Health, McGill University, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada; Biomedical Ethics Unit, McGill University, Montreal, Quebec, Canada. Electronic address:

Objective: We evaluated the effects of mental health interventions among people hospitalized with COVID-19.

Methods: We conducted a systematic review and searched 9 databases (2 Chinese-language) from December 31, 2019 to June 28, 2021. Eligible randomized controlled trials assessed interventions among hospitalized COVID-19 patients that targeted mental health symptoms. Due to the poor quality of trials, we sought to verify accuracy of trial reports including results.

Results: We identified 47 randomized controlled trials from China (N = 42), Iran (N = 4) and Turkey (N = 1) of which 21 tested the efficacy of psychological interventions, 5 physical and breathing exercises, and 21 a combination of interventions. Trial information could only be verified for 3 trials of psychological interventions (cognitive behavioral, guided imagery, multicomponent online), and these were the only trials with low risk of bias on at least 4 of 7 domains. Results could not be pooled or interpreted with confidence due to the degree of poor reporting and trial quality, the frequency of what were deemed implausibly large effects, and heterogeneity.

Conclusion: Trials of interventions to address mental health in hospitalized COVID-19 patients, collectively, are not of sufficient quality to inform practice. Health care providers should refer to existing expert recommendations and standard hospital-based practices.

Registration: PROSPERO (CRD42020179703); registered on April 17, 2020.
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http://dx.doi.org/10.1016/j.genhosppsych.2022.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993417PMC
April 2022

Hypolimnetic deoxygenation enhanced production and export of recalcitrant dissolved organic matter in a large stratified reservoir.

Water Res 2022 Jul 2;219:118537. Epub 2022 May 2.

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361012, China; Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, Xiamen University, Xiamen 361012, China; National Observation and Research Station for the Taiwan Strait Marine Ecosystem, Xiamen University, Xiamen 361012, China. Electronic address:

Global impoundment of river systems represents a major anthropogenic forcing to carbon cycling in reservoirs with seasonal thermal stratification. Currently, a quantitative and mechanistic understanding of how hypolimnetic deoxygenation in stratified reservoirs alters dissolved organic matter (DOM) cycling and lateral transport along the river continuum remains unresolved. Herein, we used optical and high-resolution mass spectrometric analyses to track seasonal and spatial compositional changes of DOM from a large, subtropical impounded river in southeast China. Aliphatic compounds were contributed by algal blooms to epilimnetic DOM during the spring/summer and by baseflow to the overall DOM pool during low-discharge periods. Deoxygenation-driven hypolimnetic mineralization enhanced in situ production of bio-refractory molecules and humic-like fluorescent DOM (FDOM) by utilizing bio-labile DOM and settling biogenic particles during periods of stratification. Production efficiency of hypolimnetic FDOM was 159-444% higher than that of the global dark ocean, and was strongly regulated by temperature and possibly substrate supply. The in situ production rate of hypolimnetic FDOM was four to five orders-of-magnitude higher than the dark ocean, with much faster turnover rates in dark inland waters versus the dark ocean. Collectively, these findings indicate that the hypolimnion is a hotspot for microbial carbon transformations, and hence an important source and pool of refractory DOM in aquatic systems. The lateral FDOM flux increased 10.8-32.1% due to hypolimnetic reservoir release during periods of stratification, highlighting the importance of incorporating hypolimnetic carbon transformations into models for carbon cycling of inland waters and the land-sea interface.
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http://dx.doi.org/10.1016/j.watres.2022.118537DOI Listing
July 2022

Long non-coding RNA TFAP2A-AS1 plays an important role in oral squamous cell carcinoma: research includes bioinformatics analysis and experiments.

BMC Oral Health 2022 05 6;22(1):160. Epub 2022 May 6.

Department of Stomatology, The Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang, 050000, Hebei Province, China.

Background: Oral squamous cell carcinoma (OSCC) is the most common neck and head malignancies, and the prognosis is not good. Studies shown that the long non-coding RNA (lncRNA) TFAP2A-AS1 is involved in the progression of multiple cancers. However, the role of lncRNA TFAP2A-AS1 in OSCC remains unclear. We aimed to explore the functions and expression in OSCC.

Methods: The lncRNA profiles for OSCC patients were acquired from the TCGA. Based on these data, the data mining of TFAP2A-AS1 in patients with OSCC were performed. The functions of TFAP2A-AS1 were determined by bioinformatics analysis. The expression and roles in cell growth were tested by RT-qPCR and MTS assay. Cell invasion and migration were tested by wound healing and transwell assays.

Results: The consequences displayed that TFAP2A-AS1 was upregulated in the TCGA datasets. The expression of TFAP2A-AS1 was higher in OSCC samples. Bioinformatics analysis shown that TFAP2A-AS1 might be associated with the P53 signaling pathway. Cell culture experiments indicated that deficiency of TFAP2A-AS1 inhibited cell growth, invasion, and migration, and overexpression of it could opposite results in SCC-25 cells.

Conclusion: The results suggested that TFAP2A-AS1 was overexpressed in OSCC cells, which could facilitate OSCC cell proliferation, migration, and invasion.
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http://dx.doi.org/10.1186/s12903-022-02203-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074241PMC
May 2022
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