Publications by authors named "Chen He"

526 Publications

Model-based evaluation of image-guided fractionated whole-brain radiation therapy in pediatric diffuse intrinsic pontine glioma xenografts.

CPT Pharmacometrics Syst Pharmacol 2021 May 3. Epub 2021 May 3.

Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN, USA.

Radiation therapy (RT) is currently the standard treatment for diffuse intrinsic pontine glioma (DIPG), the most common cause of death in children with brain cancer. A pharmacodynamic model was developed to describe the radiation-induced tumor shrinkage and overall survival in mice bearing DIPG. CD1-nude mice were implanted in the brain cortex with luciferase-labeled patient-derived orthotopic xenografts of DIPG (SJDIPGx7 H3F3A and SJDIPGx37 H3F3A ). Mice were treated with image-guided whole-brain RT at 1 or 2 Gy/fraction 5-days-on 2-days-off for a cumulative dose of 20 or 54 Gy. Tumor progression was monitored with bioluminescent imaging (BLI). A mathematical model describing BLI and overall survival was developed with data from mice receiving 2 Gy/fraction and validated using data from mice receiving 1 Gy/fraction. BLI data were adequately fitted with a logistic tumor growth function and a signal distribution model with linear radiation-induced killing effect. A higher tumor growth rate in SJDIPGx37 versus SJDIPGx7 xenografts and a killing effect decreasing with higher tumor baseline (p < 0.0001) were identified. Cumulative radiation dose was suggested to inhibit the tumor growth rate according to a Hill function. Survival distribution was best described with a Weibull hazard function in which the hazard baseline was a continuous function of tumor BLI. Significant differences were further identified between DIPG cell lines and untreated versus treated mice. The model was adequately validated with mice receiving 1 Gy/fraction and will be useful in guiding future preclinical trials incorporating radiation and to support systemic combination therapies with RT.
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http://dx.doi.org/10.1002/psp4.12627DOI Listing
May 2021

The 3D Modeling System for Bioaerosol Distribution Based on Planar Laser-Induced Fluorescence.

Sensors (Basel) 2021 Apr 8;21(8). Epub 2021 Apr 8.

Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.

Although it is quite challenging to image and analyze the spatial distribution of bioaerosols in a confined space, a three-dimensional (3D) modeling system based on the planar laser-induced fluorescence (PLIF) technique is proposed in this paper, which is designed to analyze the temporal and spatial variations of bioaerosol particles in a confined chamber. The system employs a continuous planar laser source to excite the fluoresce, and a scientific complementary metal oxide semiconductor (sCMOS) camera to capture images of 2048 × 2048 pixels at a frame rate of 12 Hz. While a sliding platform is moving back and forth on the track, a set of images are captured at different positions for 3D reconstruction. In this system, the 3D reconstruction is limited to a maximum measurement volume of about 50 cm × 29.7 cm × 42 cm, with a spatial resolution of about 0.58 mm × 0.82 mm × 8.33 mm, and a temporal resolution of 5 s. Experiments were carried out to detect the PLIF signals from fluorescein aerosols in the chamber, and then 3D reconstruction was used to visualize and analyze the diffusion of aerosol particles. The results prove that the system can be applied to clearly reconstruct the 3D distribution and record the diffusion process of aerosol particles in a confined space.
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http://dx.doi.org/10.3390/s21082607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068142PMC
April 2021

MicroRNA-27a promotes tumorigenesis in tongue squamous cell carcinoma by enhancing proliferation, migration and suppressing apoptosis.

Eur Arch Otorhinolaryngol 2021 Apr 28. Epub 2021 Apr 28.

Department of Stomatology, the Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Shijiazhuang, 050000, Hebei province, China.

Background: Tongue squamous cell carcinoma (TSCC) is a major subtype of head and neck squamous cell carcinoma (HNSCC), which is an intractable cancer with a poor prognosis. Studies have shown that microRNAs (miRNAs) play an important role in TSCC biology. However, the expression and functions of miRNAs in TSCC remain unclear.

Methods: The non-coding RNA profiles of TSCC were downloaded from the GEO database. WGCNA (Weighted gene co-expression network analysis) and differential expression miRNA (DE-miRNA) analyses were employed to identify key candidate miRNAs. miRNA expression was detected using RT-qPCR analysis. The target genes of key miRNAs were predicted. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to explore the potential functions and pathways of key miRNA. miRNA inhibitor was transfected to detect the function of miRNA. The effect of miRNA deregulation on TSCC cell proliferation and apoptosis was investigated using MTS, Annexin V-FITC/PI double staining, and flow cytometry assays.

Results: miR-27a was a key miRNA in TSCC, which was significantly up-regulated in both Cal-27 cells and malignant tissues from the TSCC patients. In addition, functional analysis showed that miR-27a was involved in the regulation of the MAPK, ERBB, and Jak-STAT signaling pathways. Moreover, RHOA and PRKACA were potential target genes of miR-27a, suggesting them as possible mediators of the tumor-promoting effect of miR-27a. Moreover, downregulation of miR-27a inhibited cell proliferation and facilitated cell apoptosis in Cal-27 cells.

Conclusion: Our findings strongly suggest that miR-27a could promote the tumorigenesis and development of TSCC, which makes it a potential new diagnostic marker and therapeutic target for TSCC.
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http://dx.doi.org/10.1007/s00405-021-06837-yDOI Listing
April 2021

Association between blood urea nitrogen and incidence of type 2 diabetes mellitus in a Chinese population: a cohort study.

Endocr J 2021 Apr 28. Epub 2021 Apr 28.

Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China.

To examine the association between blood urea nitrogen (BUN) and risk of type 2 diabetes (T2DM) among Chinese adults, we performed an ongoing cohort study of 38578 Chinese adults (56.3% males; average age, 41.6 y) who underwent repeated health check-up examinations between 2009 and 2016 and without T2DM at baseline. During follow-up, incident T2DM cases were identified based on self-report, medication use, measurements of fasting plasma glucose, 2 h post oral glucose, or haemoglobinA1c. 2009 (5.2%) cases confirmed with incident T2DM were identified during median follow-up of 3.1 years. With increasing quartiles of BUN levels, the incidences of T2DM gradually increased with 0.69%, 1.11%, 1.53%, and 1.87% for quartile 1 to quartile 4 (p trend <0.001). Compared with quartile 1, the multivariate-adjusted hazard ratios (HRs) and its 95% confidence intervals (95% CIs) for T2DM risk were 1.16 (0.97-1.38) for quartile 2, 1.28 (1.07-1.51) for quartile 3, and 1.28 (1.08-1.52) for quartile 4 (p trend = 0.005). HR for per each standard deviation increase in BUN level was 1.10 (1.04-1.16) (p trend <0.001). This association tended to be more pronounced in those with a lower body mass index at baseline (p-interaction <0.001). Our results suggested that BUN levels were positively associated with incident T2DM risk among Chinese adults. Future prospective investigations in other populations are necessary to confirm our findings.
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http://dx.doi.org/10.1507/endocrj.EJ20-0794DOI Listing
April 2021

Decline of Orientation and Direction Sensitivity in the Aging Population.

Front Neurosci 2021 7;15:643414. Epub 2021 Apr 7.

Department of Ophthalmology, First Affiliated Hospital of Anhui Medical University, Hefei, China.

While the aging population is growing, our knowledge regarding age-related deterioration of visual perception remains limited. In the present study, we investigated the effects of aging on orientation and direction sensitivity in a healthy population using a weighted up-down adaptive method to improve the efficiency and reliability of the task. A total of 57 healthy participants aged 22-72 years were included and divided into old and young groups. Raw experimental data were processed using a psychometric method to determine the differences between the two groups. In the orientation task, the threshold of the discrimination angle and bias (i.e., the difference between the perceived midpoint from the logistic function and the reference point) was increased, while the lapsing rate (i.e., 1-the maximum logistic function) did not significantly change in the old group compared with the young group. In the motion direction task, the threshold, bias, and lapsing rate were significantly increased in the old group compared with the young group. These results suggest that the decreased ability of old participants in discrimination of stimulus orientation and motion direction could be related to the impaired function of visual cortex.
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http://dx.doi.org/10.3389/fnins.2021.643414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064032PMC
April 2021

Identification of processes mobilizing organic molecules and arsenic in geothermal confined groundwater from Pliocene aquifers.

Water Res 2021 Apr 11;198:117140. Epub 2021 Apr 11.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing 100083, China; School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China.

Organic matter (OM) has been accepted as an important trigger fueling Fe(III) oxide reduction and arsenic release in the late Pleistocene-Holocene anoxic aquifers, whereas its fates and roles on arsenic mobility in the Pliocene aquifer are unclear. To fill this gap, groundwaters from a confined Pliocene aquifer (CG) and an unconfined Holocene aquifer (UG) were sampled in the Guide Basin, China, to monitor evolutions of groundwater geochemistry and OM molecular signatures along the groundwater flow path. The outcomes showed that groundwater pH, temperature, and arsenic concentrations in the CG samples generally increased along the groundwater flow path, which were much higher than those in the UG samples. The numbers and intensities of recalcitrant molecules (polycyclic aromatics and polyphenols) in the CG samples remarkably increased along the path, but relatively labile molecules (highly unsaturated and phenolic compounds and aliphatic compounds) showed the opposite trends. The arsenic-poor (<10 μg/L) UG samples contained more labile molecules than the arsenic-rich CG samples. High groundwater pH, temperature, and sediment age in the confined aquifers may be responsible for the selective mobilization of the unique polycyclic aromatics and polyphenols. The mobilized recalcitrant organic molecules may enhance arsenic release via electron shuttling, complexation, and competition. Furthermore, high temperature and pH may also facilitate arsenic desorption. The study provides molecular-scale evidences that the mobilization of recalcitrant organic molecules and arsenic were concurrent in the geothermal confined groundwater.
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http://dx.doi.org/10.1016/j.watres.2021.117140DOI Listing
April 2021

Correspondence between DOM molecules and microbial community in a subtropical coastal estuary on a spatiotemporal scale.

Environ Int 2021 Apr 23;154:106558. Epub 2021 Apr 23.

State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, College of Ocean and Earth Sciences, Xiamen University, Xiang'an Campus, Xiang'an South Road, Xiamen 361102, China; Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiang'an Campus, Xiang'an South Road, Xiamen 361102, China. Electronic address:

Dissolved organic matter (DOM) changes in quantity and quality over time and space, especially in highly dynamic coastal estuaries. Bacterioplankton usually display seasonal and spatial variations in abundance and composition in the coastal regions, and influence the DOM pool via assimilation, transformation and release of organic molecules. The change in DOM can also affect the composition of bacterial community. However, little is known on the correspondence between DOM molecules and bacterial composition, particularly through a systematic field survey. In this study, the spatiotemporal signatures of microbial communities and DOM composition in the subtropical coastal estuary of Xiamen are investigated over one and half years. The co-occurrence analysis between bacteria and DOM suggested microorganisms likely transformed the DOM from a relatively high (>400 Da) to a low (<400 Da) molecular weight, corresponding to an apparent increase in overall aromaticity. This might be the reason why microbial transformation renders "dark" organic matter visible in mass spectrometry due to more efficient ionization of microbial metabolites, as well as photodegradation processes. K- and r-strategists exhibited different correlations with two-size categories of DOM molecules owing to their different lifestyles and responses to environmental nutrient conditions. A comparison of the environmental variables and DOM composition with the microbial communities showed that the environmental/DOM variations played a more important role in shaping the microbial communities than vice versa. This study sheds light on the interactions between microbial populations and DOM molecules at the spatiotemporal scale, improving our understanding of microbial roles in marine biogeochemical cycles.
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http://dx.doi.org/10.1016/j.envint.2021.106558DOI Listing
April 2021

Service Delivery Reforms for Asian Ageing Societies: A Cross-Country Study Between Japan, South Korea, China, Thailand, Indonesia, and the Philippines.

Int J Integr Care 2021 Apr 6;21(2). Epub 2021 Apr 6.

Department of Global Health, Graduate School of Health Sciences, University of the Ryukyus, 207 Uehara, Nishihara-cho, Nakagami-gun, Okinawa 903-0213, JP.

Introduction: Japan's health policies to address the most advanced-aged society have been the target of focus in Asia, but no studies have investigated this issue using tools for cross-country comparisons.

Theory And Methods: A cross-country study design was used to compare healthcare reform policies with a framework in Japan, Korea, Thailand, China, Indonesia, and the Philippines. Data were collected via document reviews and key informant interviews.

Results: Three distinctions were identified. First, all countries except for the Philippines have policy decisions regarding reforms for the existing service delivery systems for healthcare, long-term care and welfare. Second, the most extensive service delivery reform is currently being implemented in Japan, whose system is shifting to primary health care. Third, the direction of the transformation of service delivery system is different between Thailand and China despite a similar level of ageing society. China has made progress on facility-based care integration between health and social care, whereas Thailand is focusing on home-based care.

Conclusions And Discussion: Doctor and hospital-based healthcare delivery system requires more drastic reform for an aged society. This fact implies that strengthening primary health care is not only useful for current health issues but also an investment for the aged society near future in low- and middle-income countries.
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http://dx.doi.org/10.5334/ijic.4739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034408PMC
April 2021

The Modulating Effect of Top-down Attention on the Optimal Pre-target Onset Oscillatory States of Bottom-up Attention.

Neuroscience 2021 Apr 15. Epub 2021 Apr 15.

State Key Laboratory of Cognitive Neuroscience and Learning & IDG, McGovern Institute for Brain Research, Beijing Normal University, Beijing 100875, China. Electronic address:

Research in both bottom-up and top-down attention has shown that behavioural performance is related to brain oscillations at the time of stimulus presentation: the angle of the theta phase in bottom-up attention and the inhibition of alpha oscillations in top-down attention. However, whether the conditions most favourable for bottom-up attention change with the addition of top-down cues is unclear. To explore the characteristics of favourable oscillations during bottom-up processing, in experiment 1, 36 participants completed a selective attention task (visual search) without cues. Then, in experiment 2, we examined whether favourable oscillatory characteristics were changed by top-down attentional cues; in this experiment, 62 subjects were asked to perform an attention network task. We found that without anticipation, oscillatory states that were associated with better performance were characterized by lower theta power in the frontal area, higher alpha power in the occipital area, higher beta power in the frontal area, and weaker gamma-theta amplitude-envelope coupling in the parietal area. However, some characteristics that were associated with better performance, including theta power and low beta power, were changed after the addition of different cues. In addition, there were some new characteristics related to improved performance under temporal and spatial anticipation. These results suggest that top-down attention implements a more energy-efficient strategy to process information, optimizing the process of bottom-up attention.
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http://dx.doi.org/10.1016/j.neuroscience.2021.03.036DOI Listing
April 2021

CT-Guided Microcoil Localization of Small Peripheral Pulmonary Nodules to Direct Video-Assisted Thoracoscopic Resection without the Aid of Intraoperative Fluoroscopy.

Korean J Radiol 2021 Apr 1. Epub 2021 Apr 1.

Department of Radiology, China-Japan Friendship Hospital, Beijing, China.

Objective: To evaluate the feasibility, safety, and effectiveness of CT-guided microcoil localization of solitary pulmonary nodules (SPNs) for guiding video-assisted thoracoscopic surgery (VATS).

Materials And Methods: Between June 2016 and October 2019, 454 consecutive patients with 501 SPNs who received CT-guided microcoil localization before VATS in our institution were enrolled. The diameter of the nodules was 0.93 ± 0.49 cm, and the shortest distance from the nodules to the pleura was 1.41 ± 0.95 cm. The distal end of the microcoil was placed less than 1 cm away from the nodule, and the proximal end was placed outside the visceral pleura. VATS was performed under the guidance of implanted microcoils without the aid of intraoperative fluoroscopy.

Results: All 501 nodules were marked with microcoils. The time required for microcoil localization was 12.8 ± 5.2 minutes. Microcoil localization-related complications occurred in 179 cases (39.4%). None of the complications required treatment. A total of 463 nodules were successfully resected under the guidance of implanted microcoils. VATS revealed 38 patients with dislocated microcoils, of which 28 underwent wedge resection (21 cases under the guidance of the bleeding points of pleural puncture, 7 cases through palpation), 5 underwent direct lobectomy, and the remaining 5 underwent a conversion to thoracotomy. In 4 cases, a portion of the microcoil remained in the lung parenchyma.

Conclusion: CT-guided microcoil localization of SPNs is safe and reliable. Marking the nodule and pleura simultaneously with microcoils can effectively guide the resection of SPNs using VATS without the aid of intraoperative fluoroscopy.
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http://dx.doi.org/10.3348/kjr.2020.0331DOI Listing
April 2021

Correcting a major error in assessing organic carbon pollution in natural waters.

Sci Adv 2021 Apr 14;7(16). Epub 2021 Apr 14.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing 102249, China.

Microbial degradation of dissolved organic carbon (DOC) in aquatic environments can cause oxygen depletion, water acidification, and CO emissions. These problems are caused by labile DOC (LDOC) and not refractory DOC (RDOC) that resists degradation and is thus a carbon sink. For nearly a century, chemical oxygen demand (COD) has been widely used for assessment of organic pollution in aquatic systems. Here, we show through a multicountry survey and experimental studies that COD is not an appropriate proxy of microbial degradability of organic matter because it oxidizes both LDOC and RDOC, and the latter contributes up to 90% of DOC in high-latitude forested areas. Hence, COD measurements do not provide appropriate scientific information on organic pollution in natural waters and can mislead environmental policies. We propose the replacement of the COD method with an optode-based biological oxygen demand method to accurately and efficiently assess organic pollution in natural aquatic environments.
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http://dx.doi.org/10.1126/sciadv.abc7318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046372PMC
April 2021

Single nucleotide polymorphisms in breast cancer susceptibility gene 1 are associated with susceptibility to lung cancer.

Oncol Lett 2021 May 29;21(5):424. Epub 2021 Mar 29.

Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China.

BRCA1 is a tumor suppressor that has been found to be involved DNA synthesis during cell replication. In a recent study, the single nucleotide polymorphism (SNP), rs799917, in BRCA1 was found to be associated with the development and progression of various types of tumor. In the present study, the association between rs799917 and susceptibility to lung cancer was evaluated in a Han Chinese population in the Liaoning Province of China. The BRCA1 rs799917 genotypes (C/C, C/T and T/T) were analyzed using TaqMan quantitative PCR in 682 patients with lung cancer and 694 healthy controls, and the results were analyzed using a Student's t-test, a χ test and logistic regression analysis. Individuals carrying the C/T or T/T genotype had a lower risk of lung cancer compared with those carrying the C/C genotype [odds ratio (OR), 0.741; P=0.021; and OR, 0.610; P=0.011, respectively). The C/T + T/T genotype group had an even lower risk (OR, 0.709; P=0.005) compared with that in the C/C genotype group. In the stratified analyses of non-smokers, individuals with the C/T or T/T genotype had a lower risk of developing lung cancer compared with that in those carrying the C/C genotype (OR, 0.681; P=0.013; and OR, 0.569; P=0.021, respectively). The stratified analyses of the BRCA1 rs799917 polymorphism based on pathological type, chemotherapy and radiotherapy, showed that in the squamous cell carcinoma, non-chemotherapy and non-radiotherapy subgroups, individuals with the T/T genotype had a lower risk of lung cancer compared with that in those carrying the C/C genotype (OR, 0.454; P=0.007; OR, 0.485; P=0.002; and OR, 0.599; P=0.020, respectively). In conclusion, the T allele of the rs799917 SNP in BRCA1 was associated with a lower risk of lung cancer in the ethnic Han Chinese population in Liaoning Province and may represent a protective factor against lung cancer.
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http://dx.doi.org/10.3892/ol.2021.12685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025117PMC
May 2021

The first record of exceptionally-preserved spiral coprolites from the Tsagan-Tsab formation (lower cretaceous), Tatal, western Mongolia.

Sci Rep 2021 Apr 12;11(1):7891. Epub 2021 Apr 12.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing, 100044, China.

In this paper, seven coprolites from the Lower Cretaceous of Tsagan-Tsab formation have been described. Thus, producing a significant contribution to what we perceived as the first detailed study of coprolites from the Mesozoic deposits in Mongolia. The collected coprolites encompass a total of six spiral amphipolar and one scroll coprolites. We prominently identified four new coprolite ichnotaxa, such as: Hyronocoprus tsagantsabensis and Hyronocoprus hunti, to which both are ichnosp. nov.; followed by Megakalocoprus barremianensis and Scrollocoprus tatalensis, where both are ichnogen. et ichnosp. nov. Notably, CT scans revealed that all specimens showed various amounts of bony inclusions and scales, hence, deducing that the producers could have had a low acidic digestive track and were unable to dissolve bone matters. Moreover, SEM-EDS analysis concluded its carnivorous nature, thus, pointing towards piscivorous diet. The small sized Scrollocoprus is considered to be the second findings of Mesozoic era's scroll coprolites, which contain possible plant pollens, a complete infraorbital bone, clusters of bone fragments and rhomboidal-shaped ganoid scales of the prey; and bioerosional scars have been observed on the surface. We suggest those amphipolar spiral ichnotaxa were produced by Asipenceriformes, with Pholidophoriformes as the prey, while Scrollocoprus represents fecal excrement of underived fish, possibly of sarcopterygian origins.
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http://dx.doi.org/10.1038/s41598-021-87090-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041832PMC
April 2021

Nutritional status and risk of contrast-associated acute kidney injury in elderly patients undergoing percutaneous coronary intervention.

Clin Exp Nephrol 2021 Apr 12. Epub 2021 Apr 12.

Department of Geriatric Medicine, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fujian Provincial Institute of Clinical Geriatrics, Fujian Key Laboratory of Geriatrics, Fujian Provincial Center for Geriatrics, Fuzhou, 350001, Fujian, China.

Background: This study aimed to investigate the connection between malnutrition evaluated by the Controlling Nutritional Status (CONUT) score and the risk of contrast-associated acute kidney injury (CA-AKI) in elderly patients who underwent percutaneous coronary intervention (PCI).

Methods: A total of 1308 patients aged over 75 years undergoing PCI was included. Based on the CONUT score, patients were assigned to normal (0-1), mild malnutrition (2-4), moderate-severe malnutrition group (≥ 5). The primary outcome was CA-AKI (an absolute increase in ≥ 0.3 mg/dL or ≥ 50% relative serum creatinine increase 48 h after contrast medium exposure).

Results: Overall, the incidence of CA-AKI in normal, mild, moderate-severe malnutrition group was 10.8%, 11.0%, and 27.2%, respectively (p < 0.01). Compared with moderate-severe malnutrition group, the normal group and the mild malnutrition group showed significant lower risk of CA-AKI in models adjusting for risk factors for CA-AKI and variables in univariate analysis (odds ratio [OR] = 0.48, 95% confidence interval [CI]: 0.26-0.89, p = 0.02; OR = 0.46, 95%CI: 0.26-0.82, p = 0.009, respectively). Furthermore, the relationship were consistent across the subgroups classified by risk factors for CA-AKI except anemia. The risk of CA-AKI related with CONUT score was stronger in patients with anemia. (overall interaction p by CONUT score = 0.012).

Conclusion: Moderate-severe malnutrition is associated with higher risk of CA-AKI in elderly patients undergoing PCI.
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http://dx.doi.org/10.1007/s10157-021-02061-4DOI Listing
April 2021

CALINCA-A Novel Pipeline for the Identification of lncRNAs in Podocyte Disease.

Cells 2021 Mar 20;10(3). Epub 2021 Mar 20.

German Center for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim, Im Neuenheimer Feld 669, 69120 Heidelberg, Germany.

Diseases of the renal filtration unit-the glomerulus-are the most common cause of chronic kidney disease. Podocytes are the pivotal cell type for the function of this filter and focal-segmental glomerulosclerosis (FSGS) is a classic example of a podocytopathy leading to proteinuria and glomerular scarring. Currently, no targeted treatment of FSGS is available. This lack of therapeutic strategies is explained by a limited understanding of the defects in podocyte cell biology leading to FSGS. To date, most studies in the field have focused on protein-coding genes and their gene products. However, more than 80% of all transcripts produced by mammalian cells are actually non-coding. Here, long non-coding RNAs (lncRNAs) are a relatively novel class of transcripts and have not been systematically studied in FSGS to date. The appropriate tools to facilitate lncRNA research for the renal scientific community are urgently required due to a row of challenges compared to classical analysis pipelines optimized for coding RNA expression analysis. Here, we present the bioinformatic pipeline CALINCA as a solution for this problem. CALINCA automatically analyzes datasets from murine FSGS models and quantifies both annotated and de novo assembled lncRNAs. In addition, the tool provides in-depth information on podocyte specificity of these lncRNAs, as well as evolutionary conservation and expression in human datasets making this pipeline a crucial basis to lncRNA studies in FSGS.
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http://dx.doi.org/10.3390/cells10030692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003990PMC
March 2021

Shape design of magnetic circuit of the magnetic lens based on medial axis transform.

Micron 2021 Mar 19;145:103057. Epub 2021 Mar 19.

School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing, China; Key Laboratory of Micro-Nano Measurement-Manipulation and Physics, China.

This paper proposes a method of designing a magnetic lens circuit based on axial transform theory. A unified mathematical description of the magnetic circuit profile is given within a medial axial transform in differential geometry. The objective constraint and geometric constraint equations of the magnetic circuit form a closed set of differential equations. Lastly, the optimized magnetic circuit is obtained using the solutions of the equations. The optical performance of the magnetic lens is significantly improved.
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http://dx.doi.org/10.1016/j.micron.2021.103057DOI Listing
March 2021

Opportunistic bacteria with reduced genomes are effective competitors for organic nitrogen compounds in coastal dinoflagellate blooms.

Microbiome 2021 03 24;9(1):71. Epub 2021 Mar 24.

State Key Laboratory of Marine Environmental Science, Fujian Key Laboratory of Marine Carbon Sequestration, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, People's Republic of China.

Background: Phytoplankton blooms are frequent events in coastal areas and increase the production of organic matter that initially shapes the growth of opportunistic heterotrophic bacteria. However, it is unclear how these opportunists are involved in the transformation of dissolved organic matter (DOM) when blooms occur and the subsequent impacts on biogeochemical cycles.

Results: We used a combination of genomic, proteomic, and metabolomic approaches to study bacterial diversity, genome traits, and metabolic responses to assess the source and lability of DOM in a spring coastal bloom of Akashiwo sanguinea. We identified molecules that significantly increased during bloom development, predominantly belonging to amino acids, dipeptides, lipids, nucleotides, and nucleosides. The opportunistic members of the bacterial genera Polaribacter, Lentibacter, and Litoricola represented a significant proportion of the free-living and particle-associated bacterial assemblages during the stationary phase of the bloom. Polaribacter marinivivus, Lentibacter algarum, and Litoricola marina were isolated and their genomes exhibited streamlining characterized by small genome size and low GC content and non-coding densities, as well as a smaller number of transporters and peptidases compared to closely related species. However, the core proteomes identified house-keeping functions, such as various substrate transporters, peptidases, motility, chemotaxis, and antioxidants, in response to bloom-derived DOM. We observed a unique metabolic signature for the three species in the utilization of multiple dissolved organic nitrogen compounds. The metabolomic data showed that amino acids and dipeptides (such as isoleucine and proline) were preferentially taken up by P. marinivivus and L. algarum, whereas nucleotides and nucleosides (such as adenosine and purine) were preferentially selected by L. marina.

Conclusions: The results suggest that the enriched DOM in stationary phase of phytoplankton bloom is a result of ammonium depletion. This environment drives genomic streamlining of opportunistic bacteria to exploit their preferred nitrogen-containing compounds and maintain nutrient cycling. Video abstract.
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http://dx.doi.org/10.1186/s40168-021-01022-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992965PMC
March 2021

Linking the unique molecular complexity of dissolved organic matter to flood period in the Yangtze River mainstream.

Sci Total Environ 2021 Apr 8;764:142803. Epub 2020 Oct 8.

Key Laboratory of Geoscience Big Data and Deep Resource of Zhejiang Province, School of Earth Sciences, Zhejiang University, Hangzhou, China; State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China. Electronic address:

Large rivers transport a significant amount of terrestrially derived dissolved organic matter (DOM) to coastal oceans, consisting of a critical component of the global biogeochemical cycle. Although high flow events usually introduce more terrestrial DOM than baseflow, the underlying molecular complexity and lability of DOM during high discharge are not well constrained, especially in large river ecosystems. By combining ultraviolet and fluorescent spectroscopy, and ultrahigh-resolution mass spectrometry, we found that stronger terrestrial DOM signal was detected during high discharge than normal discharge in the Yangtze River mainstream. The averaged DOC concentration was higher during high discharge than normal discharge. Optical properties confirmed higher aromaticity and relatively higher humic-like fluorescent components in DOM during high discharge. The molecular composition showed significantly higher molecular complexity, averaged molecular weight, aromaticity, relative abundances of polyphenols and highly unsaturated compounds of DOM during high discharge than normal discharge. A large set of unique molecular formulae (up to 4927) was only detected during high discharge. These unique molecular formulae were mostly lignin degradation products, likely due to more intensive soil leaching during high discharge. By comparing with incubation experiments and the Yangtze River mouth and East China Sea DOM molecular composition, some of these unique molecular formulae during high discharge are resistant to both bio- and photo-degradation, and persist during their transport to the East China Sea. Therefore, we suggest that high discharge will additionally introduce a relatively recalcitrant pool of DOM into the Yangtze River mainstream and persist during its journey to the ocean. Considering the projected increase of flood frequency, this study provides a preliminary foundation for further studies to better assess the underlying mechanisms how hydrology affect the biogeochemical cycling of DOM in large rivers.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142803DOI Listing
April 2021

Chemodiversity of water-extractable organic matter in sediment columns of a polluted urban river in South China.

Sci Total Environ 2021 Mar 1;777:146127. Epub 2021 Mar 1.

State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address:

Dissolved organic matter (DOM) in sediments of polluted rivers significantly contributes to oxygen consumption and river blackening and odorization. However, the chemodiversity of DOM at different depths or river reaches is poorly known. Here, we studied the storage and molecular-level signatures of water-extractable organic matter (WEOM) in the sediment column (0-100 cm) of the upper, middle, and lower mainstream of Maozhou River (a polluted river in Shenzhen, China, with 40 years of urbanization) using optical spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry. The sediment WEOM level increased from upstream to downstream. The relative abundances of sulfur-containing surfactants in all sediment WEOM were higher than those previously reported for surface water DOM. The WEOM in surface sediment had higher aromaticity, molecular size, and nominal oxidation state of carbon and greater signals from anthropogenic inputs than did deep sediment at the upper and middle mainstream sites. However, these characteristics varied little between surface and deep sediments at the lower mainstream site, probably due to intensive surface water and pore water interactions. The sediment WEOM at 0-40 cm in the middle mainstream showed a greater anthropogenic signature (e.g., more surfactant and dissolved black carbon contributions) than any other sediment. We demonstrate strong anthropogenic impacts on the surface sediment over decades of urbanization.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146127DOI Listing
March 2021

Predictive value of preprocedural albuminuria for contrast-induced nephropathy non-recovery in patients undergoing percutaneous coronary intervention.

Int Urol Nephrol 2021 Mar 6. Epub 2021 Mar 6.

Department of Cardiology, Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China.

Objective: The present study investigated the predictive value of albuminuria for contrast-induced nephropathy (CIN) non-recovery in patients undergoing percutaneous coronary intervention (PCI).

Methods: We retrospectively enrolled 550 consecutive patients inflicted with CIN after PCI and reassessing kidney function among 1 week-12 months between January 2012 and December 2018. Patients were stratified into three groups according to urine albumin: negative group (urine dipstick negative), trace group (urine dipstick trace) and positive group (urine dipstick ≥ 1 +). The primary outcomes were CIN non-recovery (a decrease of serum creatinine which remains ≥ 25% or 0.5 mg/dL over baseline at 1 week-12 months after PCI in patients inflicted with CIN). The odds ratio (OR) of CIN non-recovery was analyzed by logistic regression using the negative urine dipstick group as the reference group.

Results: Overall, 88 (16.0%) patients had trace urinary albumin, 74 (13.5%) patients had positive urinary albumin and 40 (7.3%) patients developed CIN non-recovery. Patients with positive urinary albumin had significantly higher incidence of CIN non-recovery [negative (3.4%), trace (11.4%) and positive (23.0%), respectively; P < 0.0001]. Multivariate analysis showed that trace and positive urinary albumin were associated with an increased risk of CIN non-recovery (trace vs negative: OR 2.88, P = 0.022; positive vs negative: OR 2.99, P = 0.021). These associations were consistent in subgroups of patients stratified by CIN non-recovery risk predictors. And CIN non-recovery was associated with an increased risk of long-term mortality during a mean follow-up period of 703 days (P < 0.001).

Conclusion: Preprocedural albuminuria was associated with CIN non-recovery in patients undergoing PCI.
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http://dx.doi.org/10.1007/s11255-021-02818-6DOI Listing
March 2021

MicroRNA‑34a‑5p serves as a tumor suppressor by regulating the cell motility of bladder cancer cells through matrix metalloproteinase‑2 silencing.

Oncol Rep 2021 Mar 24;45(3):911-920. Epub 2020 Dec 24.

Division of Urology, Department of Surgery, Shin Kong Wu Ho‑Su Memorial Hospital, Taipei 11102, Taiwan, R.O.C.

Bladder cancer (BC), a common urologic cancer, is the fifth most frequently diagnosed tumor worldwide. hsa‑miR‑34a displays antitumor activity in several types of cancer. However, the functional mechanisms underlying hsa‑miR‑34a in BC remains largely unknown. We observed that hsa‑mir‑34a levels were significantly and negatively associated with clinical disease stage as well as regional lymph node metastasis in human BC. In a series of in vitro investigations, overexpression of hsa‑miR‑34a inhibited cell migration and invasion in BC cell lines 5637 and UMUC3 as detected by Transwell assays. We further found that hsa‑miR‑34a inhibited cell migration and invasion by silencing matrix metalloproteinase‑2 (MMP‑2) expression and thus interrupting MMP‑2‑mediated cell motility. Our analysis of BC datasets from The Cancer Genome Atlas database revealed a negative correlation between hsa‑miR‑34a and MMP‑2. Moreover, higher MMP‑2 protein expression was observed in the BC tissues when compared with that noted in the normal tissue. MMP‑2 levels were also significantly associated with clinical disease stage and poor survival rate in human BC. These findings indicate that MMP‑2 plays a critical role in regulating BC progression. Therefore, hsa‑miR‑34a is a promising treatment to target MMP‑2 for the prevention and inhibition of cell migration and invasion in BC.
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http://dx.doi.org/10.3892/or.2020.7910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859909PMC
March 2021

Volatile DMNT directly protects plants against by disrupting the peritrophic matrix barrier in insect midgut.

Elife 2021 Feb 18;10. Epub 2021 Feb 18.

The National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, China.

Insect pests negatively affect crop quality and yield; identifying new methods to protect crops against insects therefore has important agricultural applications. Our analysis of transgenic plants showed that overexpression of , encoding the key biosynthetic enzyme for the natural plant product (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), led to a significant resistance against a major insect pest, . DMNT treatment severely damaged the peritrophic matrix (PM), a physical barrier isolating food and pathogens from the midgut wall cells. DMNT repressed the expression of in midgut cells, and knocking down resulted in PM rupture and death. A 16S RNA survey revealed that DMNT significantly disrupted midgut microbiota populations and that midgut microbes were essential for DMNT-induced killing. Therefore, we propose that the midgut microbiota assists DMNT in killing . These findings may provide a novel approach for plant protection against .
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http://dx.doi.org/10.7554/eLife.63938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924945PMC
February 2021

A First-in-Class, Highly Selective and Cell-Active Allosteric Inhibitor of Protein Arginine Methyltransferase 6.

J Med Chem 2021 Apr 16;64(7):3697-3706. Epub 2021 Feb 16.

Mount Sinai Center for Therapeutics Discovery, Departments of Pharmacological Sciences and Oncological Sciences, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, United States.

Protein arginine methyltransferase 6 (PRMT6) catalyzes monomethylation and asymmetric dimethylation of arginine residues in various proteins, plays important roles in biological processes, and is associated with multiple cancers. To date, a highly selective PRMT6 inhibitor has not been reported. Here we report the discovery and characterization of a first-in-class, highly selective allosteric inhibitor of PRMT6, (SGC6870). is a potent PRMT6 inhibitor (IC = 77 ± 6 nM) with outstanding selectivity for PRMT6 over a broad panel of other methyltransferases and nonepigenetic targets. Notably, the crystal structure of the PRMT6- complex and kinetic studies revealed binds a unique, induced allosteric pocket. Additionally, engages PRMT6 and potently inhibits its methyltransferase activity in cells. Moreover, 's enantiomer, (SGC6870N), is inactive against PRMT6 and can be utilized as a negative control. Collectively, - is a well-characterized PRMT6 chemical probe and a valuable tool for further investigating PRMT6 functions in health and disease.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035306PMC
April 2021

Microbial transformation of distinct exogenous substrates into analogous composition of recalcitrant dissolved organic matter.

Environ Microbiol 2021 Feb 8. Epub 2021 Feb 8.

State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.

Oceanic dissolved organic matter (DOM) comprises a complex molecular mixture which is typically refractory and homogenous in the deep layers of the ocean. Though the refractory nature of deep-sea DOM is increasingly attributed to microbial metabolism, it remains unexplored whether ubiquitous microbial metabolism of distinct carbon substrates could lead to similar molecular composition of refractory DOM. Here, we conducted microbial incubation experiments using four typically bioavailable substrates (L-alanine, trehalose, sediment DOM extract, and diatom lysate) to investigate how exogenous substrates are transformed by a natural microbial assemblage. The results showed that although each-substrate-amendment induced different changes in the initial microbial assemblage and the amended substrates were almost depleted after 90 days of dark incubation, the bacterial community compositions became similar in all incubations on day 90. Correspondingly, revealed by ultra-high resolution mass spectrometry, molecular composition of DOM in all incubations became compositionally consistent with recalcitrant DOM and similar toward that of DOM from the deep-sea. These results indicate that while the composition of natural microbial communities can shift with substrate exposures, long-term microbial transformation of distinct substrates can ultimately lead to a similar refractory DOM composition. These findings provide an explanation for the homogeneous and refractory features of deep-sea DOM.
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http://dx.doi.org/10.1111/1462-2920.15426DOI Listing
February 2021

Association between serum prostate-specific antigen concentrations and the risk of developing type 2 diabetes mellitus in Chinese men: A cohort study.

J Diabetes Investig 2021 Feb 5. Epub 2021 Feb 5.

Department of Epidemiology, School of Medicine, Jinan University, Guangzhou, China.

Aims/introduction: The current literature suggests that men with diabetes have a lower prostate-specific antigen concentration than men without diabetes, but the causal association remains unclear. We aimed to investigate the association between serum prostate-specific antigen concentrations and the risk of type 2 diabetes mellitus in a cohort study of a Chinese population.

Materials And Methods: We designed a cohort study that comprised 16,811 initially non-diabetic Chinese men who received annual health checkups between 2009 and 2016. The outcome of this study was type 2 diabetes mellitus, identified by medical diagnosis, self-reportage, medication use, fasting glucose, 2-h post oral glucose or glycated hemoglobin measurements. Cox proportional hazards models were carried out to evaluate the association.

Results: During a median follow-up period of 3.8 years (interquartile range 1.91-5.73 years), 1,260 participants developed incident type 2 diabetes mellitus. The multivariable model, adjusted for various potential confounders, showed that serum prostate-specific antigen concentrations were inversely related to type 2 diabetes mellitus risk (P for trend = 0.014). Compared with the lowest quartile of serum prostate-specific antigen, the hazard ratio and 95% confidence intervals of type 2 diabetes mellitus risk for quartile 2-4 were 0.84 (0.66-1.07), 0.75 (0.59-0.94) and 0.77 (0.62-0.96), respectively. Subgroup analyses suggested the inverse relationship was more prominent in overweight or obese participants (P for interaction = 0.013).

Conclusions: High serum prostate-specific antigen concentration was associated with a low risk of type 2 diabetes mellitus in Chinese men. Future studies are required to confirm these findings and investigate underlying mechanisms.
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http://dx.doi.org/10.1111/jdi.13521DOI Listing
February 2021

Characterization of lipid profiling in three parts (muscle, head and viscera) of tilapia (Oreochromis niloticus) using lipidomics with UPLC-ESI-Q-TOF-MS.

Food Chem 2021 Jun 11;347:129057. Epub 2021 Jan 11.

College of Food Science and Engineering, Hainan University, Haikou 570228, China; Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, Haikou 570228, China; Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 570228, China. Electronic address:

A lipidomic evaluation was performed on the tilapia muscle, head and viscera, including studying the composition, distribution and stereospecific characteristics of fatty acids and lipid species. The head and viscera lipids were significantly richer than the muscle lipids. Triacylglycerols were the predominant fraction (over 80% of total lipid in the muscle and head). Additionally, polyunsaturated fatty acids had higher percentages in phospholipids (30.35-52.05% of total fatty acids) than in triacylglycerols (18.11-25.15%). The C52:2 and C52:3 were the most abundant triacylglycerols, which indicates the potential application in infant food. Moreover, 622, 530 and 513 lipids were identified using ultraperformance liquid chromatography-quadrupole time-of-flight-mass spectrometry in the muscle, head and viscera, respectively. The three tilapia parts were distinguished using multivariate analysis. Five fatty acids and 33 lipid species were considered as the potential biomarkers. This comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in tilapia consumption and processing.
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http://dx.doi.org/10.1016/j.foodchem.2021.129057DOI Listing
June 2021

Preinvisible: An Early-Career Perspective on a Midcareer Phenomenon.

Perm J 2020 09;24:1-4

Department of Emergency Medicine, Thomas Jefferson University, Philadelphia, PA.

In this commentary, a female early-career academic physician reflects on her experiences with microinequities in the workplace. Using a recent publication describing the experience of midcareer academic women physicians as a launching point, the author discusses the experiences that early-career women in medicine commonly have. In training and early career, women are exposed to subtle barriers, aggressions, and inequities, which build over time. By midcareer, some women leave medicine or if they remain in medicine, they have likely not reached the salary or promotion levels of men. Ultimately, the author questions if trainees and early-career women in academic medicine are simply in a "preinvisible" phase of their careers. Ways to address the microinequities are offered.
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http://dx.doi.org/10.7812/TPP/20.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849261PMC
September 2020

LRP1-mediated pH-sensitive polymersomes facilitate combination therapy of glioblastoma in vitro and in vivo.

J Nanobiotechnology 2021 Jan 22;19(1):29. Epub 2021 Jan 22.

Medical School of Southeast University, 87 Dingjiaqiao Road, Nanjing, China.

Background: Glioblastoma (GBM) is the most invasive primary intracranial tumor, and its effective treatment is one of the most daunting challenges in oncology. The blood-brain barrier (BBB) is the main obstacle that prevents the delivery of potentially active therapeutic compounds. In this study, a new type of pH-sensitive polymersomes has been designed for glioblastoma therapy to achieve a combination of radiotherapy and chemotherapy for U87-MG human glioblastoma xenografts in nude mice and significantly increased survival time.

Results: The Au-DOX@PO-ANG has a good ability to cross the blood-brain barrier and target tumors. This delivery system has pH-sensitivity and the ability to respond to the tumor microenvironment. Gold nanoparticles and doxorubicin are designed as a complex drug. This type of complex drug improve the radiotherapy (RT) effect of glioblastoma. The mice treated with Au-DOX@PO-ANG NPs have a significant reduction in tumor volume.

Conclusion: In summary, a new pH-sensitive drug delivery system was fabricated for the treatment of glioblastoma. The new BBB-traversing drug delivery system potentially represents a novel approach to improve the effects of the treatment of intracranial tumors and provides hope for glioblastoma treatment.
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http://dx.doi.org/10.1186/s12951-020-00751-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821499PMC
January 2021

Closed-loop transcranial magnetic stimulation of real-time EEG based on the AR mode method.

Biomed Phys Eng Express 2020 03 13;6(3):035010. Epub 2020 Mar 13.

State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, People's Republic of China.

Objective: Transcranial magnetic stimulation (TMS) as a safe, noninvasive brain regulation technology has been gradually applied to clinical treatment. Traditional TMS devices do not adjust output based on real-time brain activity information when regulating the cerebral cortex, but the current activity information from the brain, especially the EEG phase, may affect the stimulation effect. It is necessary to calculate the synchronous EEG phase during TMS.

Approach: In this study, a set of closed-loop TMS device a fast EEG phase prediction algorithm based on the AR model was designed to meet the demand. EEG data for twenty-seven healthy college students were collected to verify the accuracy of the algorithm.

Main Results: The calculation results showed that the prediction accuracy of the AR model algorithm is better than that of the conventional algorithm when the model order is lower, and the prediction accuracy will increase with improvements in the signal quality.

Significance: When the experimental environment is good, the EEG data with a high SNR can be recorded, and when the order of the AR model is properly set, the prediction algorithm can make correct judgments most of the time and the stimulation pulse can be output when the EEG phase reaches a set value.
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http://dx.doi.org/10.1088/2057-1976/ab4a1cDOI Listing
March 2020

Saffloflavone, a new flavonoid from the flowers of L. and its cardioprotective activity.

Nat Prod Res 2021 Jan 12:1-6. Epub 2021 Jan 12.

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, PR China;

A new flavonoid, saffloflavone , along with six known compounds, kaempferol-3--rutinoside, kaempferol-3--sophoroside, quercetin-3---d-glucoside, quercetin-7---d-glucoside, luteolin-7---d-glucoside and kaempferol 3---d-glucoside were isolated from the flowers of L. All the structures were determined by interpretation of their spectroscopic data. The cardioprotective effects of all the isolates against oxidative stress of H9c2 cells induced by HO were investigated. The results showed that compounds - exhibited protective effects against of H9c2 cells injury induced by HO
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http://dx.doi.org/10.1080/14786419.2020.1855167DOI Listing
January 2021