Publications by authors named "Chen Fang"

2,014 Publications

  • Page 1 of 1

Effect of micro-aerobic conditions based on semipermeable membrane-covered on greenhouse gas emissions and bacterial community during dairy manure storage at industrial scale.

Environ Pollut 2022 Jan 23:118879. Epub 2022 Jan 23.

Engineering Laboratory for AgroBiomass Recycling & Valorizing, College of Engineering, China Agricultural University, Beijing 100083, China. Electronic address:

This study evaluated the greenhouse gas emissions of solid dairy manure storage with the micro-aerobic group (MA; oxygen concentration <5%) and control group (CK; oxygen concentration <1%), and explained the difference in greenhouse gas emissions by exploring bacterial community succession. The results showed that the MA remained the micro-aerobic conditions, which the maximum and average oxygen concentrations were 4.1% and 1.9%, respectively; while the average oxygen concentrations of the CK without intervention management was 0.5%. Compared with the CK, carbon dioxide and methane emissions in MA were reduced by 78.68% and 99.97%, respectively, and nitrous oxide emission was increased by almost three times with a small absolute loss, but total greenhouse gas emissions decreased by 91.23%. BugBase analysis showed that the relative abundance of aerobic bacteria in CK decreased to 0.73% on day 30, while that in MA increased to 6.56%. Genus MBA03 was significantly different between the two groups (p < 0.05) and was significantly positively correlated with carbon dioxide and methane emissions (p < 0.05). A structural equation model also revealed that the oxygen concentration and MBA03 of the MA had significant direct effects on methane emission rate (p < 0.001). The research results could provide theoretical basis and measures for directional regulation of greenhouse gas emission reduction during dairy manure storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2022.118879DOI Listing
January 2022

Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response.

Elife 2022 Jan 26;11. Epub 2022 Jan 26.

College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.

Under starvation conditions, bacteria tend to slow down their translation rate by reducing rRNA synthesis, but the way they accomplish that may vary in different bacteria. In species, transcription of rRNA is activated by the RNA polymerase (RNAP) accessory transcription factor CarD, which interacts directly with RNAP to stabilize the RNAP-promoter open complex formed on rRNA genes. The functions of CarD have been extensively studied, but the mechanisms that control its expression remain obscure. Here, we report that the level of CarD was tightly regulated when mycobacterial cells switched from nutrient-rich to nutrient-deprived conditions. At the translational level, an antisense RNA of (AscarD) was induced in a SigF-dependent manner to bind with mRNA and inhibit CarD translation, while at the post-translational level, the residual intracellular CarD was quickly degraded by the Clp protease. AscarD thus worked synergistically with Clp protease to decrease the CarD level to help mycobacterial cells cope with the nutritional stress. Altogether, our work elucidates the regulation mode of CarD and delineates a new mechanism for the mycobacterial starvation response, which is important for the adaptation and persistence of mycobacterial pathogens in the host environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.73347DOI Listing
January 2022

Whole mulberry leaves as a promising functional food: From the alteration of phenolic compounds during spray drying and in vitro digestion.

J Food Sci 2022 Jan 24. Epub 2022 Jan 24.

State Key Laboratory of Food Science and Technology, College of Food Science, Nanchang University, Nanjing East Road, Nanchang, Jiangxi, 330047, China.

This study investigated the potential of processing whole mulberry leaves as nutraceutical foods rich in phenolic compounds by spray drying with different drying aids. The results indicated that the spray-dried product 6 (24.2% whey protein isolate [WPI], 33.3% mulberry leaves solid, 38.7% maltodextrin, 3.8% soybean lecithin) with high WPI/mulberry leaves solid ratio possessed the best physical properties, the highest total phenolic compounds level and antioxidant capacity among all the products. Specifically, free chlorogenic acid and rutin were increased by two to three times, but free isoquercitrin and astragalin lost more than 50% in product 6 compared with fresh mulberry leaves. For in vitro digestion, rutin, isoquercitrin, and astragalin (the antioxidative phenolic compounds in mulberry leaves) showed higher bioaccessibility than chlorogenic acid (p < 0.05) in product 6. Meanwhile, the phenolic compounds bioaccessibility of product 6 was 10-20 times higher than that of fresh whole mulberry leaves. Considering the increased level and bioaccessibility of phenolic compounds, whole mulberry leaves could be developed as potential functional foods by spray drying under the protection of WPI. PRACTICAL APPLICATION: The spray-dried whole mulberry leaves can be consumed as a beverage, meal replacement powder, or used as additive during food processing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.16015DOI Listing
January 2022

miR-18a-3p and Its Target Protein HuR May Regulate Myogenic Differentiation in Immune-Mediated Necrotizing Myopathy.

Front Immunol 2021 5;12:780237. Epub 2022 Jan 5.

Department of Rheumatology, Key Laboratory of Myositis, China-Japan Friendship Hospital, Beijing, China.

Immune-mediated necrotizing myopathy (IMNM) is characterized by manifestation of myonecrosis and regeneration of muscle fibers; however, the underlying pathogenesis remains unclear. This study aimed to investigate the role and mechanism of miR-18a-3p and its target RNA-binding protein HuR in IMNM. HuR and miR-18a-3p levels were detected in the skeletal muscles of 18 patients with IMNM using quantitative reverse-transcription real-time polymerase chain reaction (qRT-PCR) and western blotting analysis. Human myoblasts were transfected with small interfering RNA targeting HuR and miR-18a-3p mimic or inhibitor. Myogenic differentiation markers, myogenin and myosin heavy chain, were analyzed by qRT-PCR, western blotting analysis, and immunofluorescence staining. The results showed that miR-18a-3p was upregulated (p=0.0002), whereas HuR was downregulated (p=0.002) in the skeletal muscles of patients with IMNM. The expression of miR-18a-3p in patients with IMNM was negatively correlated with those of HuR (r = -0.512, p = 0.029). We also found that disease activity was positively correlated with HuR expression ( = 0.576, = 0.012) but muscle activity was negatively correlated with miR-18a-3p expression ( = -0.550, = 0.017). Besides, bioinformatics analysis and dual-luciferase reporter assays suggested that miR-18a-3p could directly target HuR. Cellular experiments showed that overexpression of miR-18a-3p inhibited myogenic differentiation by targeting HuR, whereas inhibition of miR-18a-3p led to opposite results. Therefore, miR-18a-3p and its target protein HuR may be responsible for modulating the myogenic process in IMNM and can thus be therapeutic targets for the same.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.780237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766969PMC
January 2022

The Correlation Between Gene Polymorphism and Clopidogrel Resistance in People of the Hui and Han Nationalities.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296211073272

The First People's Hospital of Yinchuan, Yinchuan, China.

To investigate the differences in the correlation between multidrug resistance protein 1 () () gene polymorphism and clopidogrel resistance in patients of the Hui and Han nationalities with percutaneous coronary intervention (PCI). A total of 377 subjects (154 people of Hui nationality, 223 people of Han nationality) with PCI were enrolled in the study. Each patient's platelet aggregation rate was induced by adenosine diphosphate and measured using light turbidimetry. Based on the results, the patients were divided into two groups: a clopidogrel resistance (CR) group and a non-clopidogrel resistance (NCR) group. Restrictive fragment-length polymorphism polymerase chain reaction technology was then used to determine the genotype and alleles at two loci (C3435 T[rs1045642] and C1236 T[rs1128503]), calculate the frequencies of the genotype and alleles at these two loci, and conduct correlation analysis. The incidence rate of clopidogrel resistance was 23.4%, and the frequencies of the TT genotype and T allele at C3435 T for patients of both nationalities were significantly higher in the CR group than in the NCR group (P < 0.05). There were no significant differences between the two groups in genotype or allele frequency at C1236 T. There was a significant difference in the distribution of C1236 T polymorphism between the two nationalities (P < 0.05), but there was no significant difference between the two nationalities in C3435 T polymorphism. Patients with a T allele at C3435 T are more likely to show clopidogrel resistance, and no significant differences were identified in C3435 T gene polymorphism between the two nationalities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/10760296211073272DOI Listing
January 2022

Bortezomib-Encapsulated Dual Responsive Copolymeric Nanoparticles for Gallbladder Cancer Targeted Therapy.

Adv Sci (Weinh) 2022 Jan 23:e2103895. Epub 2022 Jan 23.

Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, China.

Gallbladder cancer (GBC) is a rare but the most malignant type of biliary tract tumor. It is usually diagnosed at an advanced stage and conventional treatments are unsatisfactory. As a proteasome inhibitor, bortezomib (BTZ) exhibits excellent antitumor ability in GBC. However, the long-term treatment efficacy is limited by its resistance, poor stability, and high toxicity. Herein, BTZ-encapsulated pH-responsive copolymeric nanoparticles with estrone (ES-NP ) for GBC-specific targeted therapy is reported. Due to the high estrogen receptor expression in GBC, ES-NP can rapidly enter the cells and accumulate near the nucleus via ES-mediated endocytosis. Under acidic tumor microenvironment (TME) and 808 nm laser irradiation, BTZ is released and ROS is generated by Ce6 to destroy the "bounce-back" response pathway proteins, such as DDI2 and p97, which can effectively inhibit proteasomes and increase apoptosis. Compared to the traditional treatment using BTZ monotherapy, ES-NP can significantly impede disease progression at lower BTZ concentrations and improve its resistance. Moreover, ES-NP demonstrates similar antitumor abilities in patient-derived xenograft animal models and five other types of solid tumor cells, revealing its potential as a broad-spectrum antitumor formulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202103895DOI Listing
January 2022

Temperature Trajectory Sub-Phenotypes and The Immuno-Inflammatory Response In Pediatric Sepsis.

Shock 2021 Dec 27. Epub 2021 Dec 27.

Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia and Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA Pediatric Sepsis Program, Children's Hospital of Philadelphia, Philadelphia, PA Center for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA Flow Cytometry Research Core, Children's Hospital of Philadelphia, Philadelphia, PA, USA Department of Pediatrics, Children's Hospital of Philadelphia and the Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.

Objective: Heterogeneity has hampered sepsis trials, and sub-phenotyping may assist with enrichment strategies. However, biomarker-based strategies are difficult to operationalize. Four sub-phenotypes defined by distinct temperature trajectories in the first 72 hours have been reported in adult sepsis. Given the distinct epidemiology of pediatric sepsis, the existence and relevance of temperature trajectory-defined sub-phenotypes in children is unknown. We aimed to classify septic children into de novo sub-phenotypes derived from temperature trajectories in the first 72 hours, and compare cytokine, immune function, and immunometabolic markers across subgroups.

Methods: This was a secondary analysis of a prospective cohort of 191 critically ill septic children recruited from a single academic pediatric intensive care unit. We performed group-based trajectory modeling using temperatures over the first 72 hours of sepsis to identify latent profiles. We then used mixed effects regression to determine if temperature trajectory-defined sub-phenotypes were associated with cytokine levels, immune function, and mitochondrial respiration.

Results: We identified four temperature trajectory-defined sub-phenotypes: hypothermic, normothermic, hyperthermic fast-resolvers, and hyperthermic slow-resolvers. Hypothermic patients were less often previously healthy and exhibited lower levels of pro- and anti-inflammatory cytokines and chemokines. Hospital mortality did not differ between hypothermic children (17%) and other sub-phenotypes (3 to 11%; p = 0.26).

Conclusions: Critically ill septic children can be categorized into temperature trajectory-defined sub-phenotypes that parallel adult sepsis. Hypothermic children exhibit a blunted cytokine and chemokine profile. Group-based trajectory modeling has utility for identifying subtypes of clinical syndromes by incorporating readily available longitudinal data, rather than relying on inputs from a single timepoint.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SHK.0000000000001906DOI Listing
December 2021

In-situ photodeposition of cadmium sulfide nanocrystals on manganese dioxidenanorods with rich oxygen vacancies for boosting water-to-oxygen photooxidation.

J Colloid Interface Sci 2022 Jan 12;613:764-774. Epub 2022 Jan 12.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Department of Chemistry, Zhejiang Normal University, Jinhua 321004, China; Hangzhou Institute of Advanced Studies, Zhejiang Normal University, Hangzhou 311231, China. Electronic address:

Carrier separation and surface reaction kinetic are two main bottlenecks limiting photocatalytic efficiency of photocatalysts towards an industrial level. In this regard, heterostructures and defects engineering have been proven to be effective strategies for addressing the two issues. However, the integrated construction of heterostructures and defects has been reported rarely. Herein, a facile in-situ photodeposition strategy has been developed to grow CdS nanocrystals on MnO nanorods with rich oxygen vacancies (V) as a direct Z-scheme photocatalyst for boosting water oxidation. It has been found that the Cd ions accept photoelectrons from MnOunder irradiation for the in-situ growth of CdS nanocrystals, which enables a close contact between the two components, providing high-speed electron-transport channels for photocatalysis. Meanwhile, the photooxidation half reactions extract surface lattice oxygen, leading to the increase of V content in MnO, which supplies abundant active sites for oxygen evolution. Owing to the synergistic effects of V and Z-scheme systems, the optimized MnO/CdS photocatalyst displays a dramatically enhanced photocatalytic activity with an O production rate of 779 μmol gh under visible-light irradiation without any cocatalysts, which is 2.33 times higher than the bare MnO. This work reveals the cooperative manipulation of V and CdS nanocrystals on MnO for achieving efficient photocatalysis, providing new insights into the construction of high-performance photocatalysts via a combined strategy of Z-scheme heterostructures and surface defects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2022.01.073DOI Listing
January 2022

In utero exposure to methylmercury impairs cognitive function in adult offspring: Insights from proteomic modulation.

Ecotoxicol Environ Saf 2022 Jan 17;231:113191. Epub 2022 Jan 17.

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, PR China. Electronic address:

Methylmercury (MeHg) is a hazardous substance that has unique neurodevelopmental toxic effects. However, its molecular alteration profile, sensitive response biomarkers, and mechanism of neuronal injury remain largely unknown. Here, the effects of intrauterine methylmercury chloride (low-, medium- and high-dose groups: 0.6 mg/kg/d, 1.2 mg/kg/d, 2.4 mg/ kg /d, respectively) exposure on learning and memory were assessed in offspring rats by behavioral tests, pathological analysis and hippocampal proteomic analysis. The results suggested that intrauterine MeHg exposure impairs spatial learning and memory and leads a significant reduction in the number and dispersion scattered arrangement in the hippocampus of offspring. Furthermore, in the tandem mass tag-based proteomics analysis, compared with the control group, a total of 74 differentially expressed proteins (DEPs) were found in the MeHg exposure groups; specifically, 32 down-regulated and 42 up-regulated proteins were identified. In addition, the pathways enrichment analysis indicated that these DEPs are implicated in several biological processes, such as synaptic plasticity and energy metabolism, as well as various molecular functional categories. Simultaneously, MeHg reduced the postsynaptic density, diminished the active zone, amplified the synaptic cleft and changed the synaptic interface of pyramidal cells. Western blot analysis further revealed that MeHg significantly reduced the levels of Forkhead box protein (FOXP2), Synaptophysin (SYP) and Postsynaptic density protein 95 (PSD-95), and down-regulated the N-methyl-D-aspartate receptor 1 (NMDAR1), N-methyl-D-aspartate receptor 2 A (NR2A) and N-methyl-D-aspartate receptor 2B (NR2B). In general, from a functional perspective, most overlapping proteins were related to NMDA receptor-mediated glutamatergic signaling, which is an excitotoxicity mechanism known to influence learning and memory. These discoveries contribute to our understanding of the relationship between MeHg and cognitive deficits and provide insight into the protein mediators of this relationship and possible prospective early biomarkers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2022.113191DOI Listing
January 2022

Genome-wide association study reveals novel loci for adult type 1 diabetes in a 5-year nested case-control study.

World J Diabetes 2021 Dec;12(12):2073-2086

Department of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, Suzhou 215123, Jiangsu Province, China.

Background: Type 1 diabetes (T1D) is a severe and prevalent metabolic disease. Due to its high heredity, an increasing number of genome-wide association studies have been performed, most of which were from hospital-based case-control studies with a relatively small sample size. The association of single nucleotide polymorphisms (SNPs) and T1D has been less studied and is less understood in natural cohorts.

Aim: To investigate the significant variants of T1D, which could be potential biomarkers for T1D prediction or even therapy.

Methods: A genome-wide association study (GWAS) of adult T1D was performed in a nested case-control study (785 cases 804 controls) from a larger 5-year cohort study in Suzhou, China. Potential harmful or protective SNPs were evaluated for T1D. Subsequent expression and splicing quantitative trait loci (eQTL and sQTL) analyses were carried out to identify target genes modulated by these SNPs.

Results: A harmful SNP for T1D, rs3117017 [odds ratio (OR) = 3.202, 95% confidence interval (CI): 2.296-4.466, = 9.33 × 10] and three protective SNPs rs55846421 (0.113, 0.081-0.156, 1.76 × 10), rs75836320 (0.283, 0.205-0.392, 1.07 × 10), rs362071 (0.568, 0.495-0.651, 1.66 × 10) were identified. Twenty-two genes were further identified as potential candidates for T1D onset.

Conclusion: We identified a potential genetic basis of T1D, both protective and harmful, using a GWAS in a larger nested case-control study of a Chinese population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4239/wjd.v12.i12.2073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696645PMC
December 2021

Recombinant protein -derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis.

World J Gastroenterol 2021 Dec;27(46):7982-7994

Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China.

Background: Inflammatory bowel disease (IBD) affects millions of people worldwide and has emerged as a growing problem in industrialized nations. The lack of therapeutic targets has limited the treatment of IBD. Studies found that parasitic nematode infections can ameliorate clinical and experimental colitis. Our previous study found that rSj16, a 16-kDa secreted protein of produced by , has protective effects on dextran sulfate sodium (DSS)-induced colitis in mice. Apoptosis is an important factor in the pathogenesis of colitis. However, it is not clear whether the effect of rSj16 on colitis is related to apoptosis.

Aim: To investigate whether the protective effects of rSj16 on colitis is related to apoptosis and its mechanism.

Methods: colitis was induced by DSS. The severity of colitis was assessed. WB was used to detect the changes of apoptosis-related genes in colon tissues. Q-PCR was used to detect the changes of miRNA-217-5p and HNF1B. WB was used to detect the changes of apoptosis-related genes in intestinal epithelial cells. TUNNEL staining and flow cytometry were used to detect cell apoptosis.

Results: rSj16 attenuates clinical activity in DSS-induced colitis mice. TUNNEL staining and WB results showed that apoptosis was increased in colon tissue after treatment with DSS, and the apoptosis of colon tissue was significantly reduced after treatment with rSj16. Compared with normal mice, the expression of miR-217-5p was increased in colon tissue of DSS-induced colitis mice. In addition, the miR-217-5p target gene was decreased after administration of DSS. After treatment with rSj16, the expression of miR-217-5p was decreased and the expression of HNF1B was increased compared with the DSS-treated group. When Etoposide was used in combination with miR-217-5p mimic on MODE-K cells, the expression of cleaved-Caspase-3 and Bax was increased, and Bcl-2 was decreased compared with only Etoposide treatment, the expression of HNF1B was significantly reduced, suggesting that miR-217-5p acts as a pro-apoptotic in colon epithelial cells and down-regulates the target gene . After rSj16 administration in MODE-K cells, miR-217-5p expression was significantly decreased, HNF1B expression was increased, and apoptosis was reduced.

Conclusion: The protective effects of rSj16 on colitis is related to apoptosis and miRNA-217-5p may be a further target for therapeutic intervention against IBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3748/wjg.v27.i46.7982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678816PMC
December 2021

Synthesis of methyl glycolate by hydrogenation of dimethyl oxalate with a P modified Co/SiO catalyst.

Chem Commun (Camb) 2022 Jan 19. Epub 2022 Jan 19.

Hangzhou Institute of Advanced studies, Zhejiang Normal University, 1108 Gengwen Road, Hangzhou 311231, China.

A P-modified Co/SiO catalyst was reported for the first time in the selective hydrogenation of dimethyl oxalate (DMO) to methyl glycolate (MG) reaction and the synthesized CoP/SiO exhibited 94.6% conversion of DMO and 88.1% selectivity to MG during a 300 h continuous test. The doping element of P in the catalyst was indispensable and played an important role in improving the catalytic performance of the Co/SiO catalyst.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc07003gDOI Listing
January 2022

Mitigation effects of high methoxyl pectin on acrylamide formation in the Maillard model system.

Food Chem 2022 Jan 10;378:132095. Epub 2022 Jan 10.

College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, Beijing Key Laboratory for Food Non-thermal Processing, China Agricultural University, Beijing 100083, China. Electronic address:

Acrylamide (AA) is a potential carcinogen formed during the process of food heating. Pectin is natural food additive widely presented in fruits and vegetables. This study aimed at investigating the influence of the addition of high methoxyl apple pectin (esterification degree: 82.6%) on AA inhibition in the asparagine (Asn)/glucose (Glc) model system. Results showed that temperature (120-180 °C), pH value (6.0-7.2), pectin addition (0.2-1.0%, w/v), substrate concentration (0.01-0.5 M) and molar ratio of Asn/Glc (5:1-1:10) had significant influence on inhibition of pectin on AA formation. With adding 1.0% (w/v) pectin, the pH value, Glc consumption and Schiff base abundance declined in Asn/Glc model system. Moreover, heating treatment decreased the pH value, molecular weight, esterification degree and galacturonic acid content of pectin. Finally, the pectin degradation product was identified, which might compete with Glc for Asn in Maillard reaction, led to AA reduction. This study provided distinct evidence for controlling AA formation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.132095DOI Listing
January 2022

Remote cyclic compression ameliorates myocardial infarction injury in rats via AMPK-dependent pathway.

Microvasc Res 2022 Jan 15:104313. Epub 2022 Jan 15.

School of Acupuncture and Tuina, School of Regimen and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address:

Background: Remote ischemic conditioning (RIC) displays a cardioprotective role in acute myocardial infarction (AMI). Since interruption of blood vessel is not an essential trigger of remote cardioprotection, tissue compression may play a prominent part in the effect. The purpose of this study was to confirm the protective effect of tissue compression on AMI and the underlying mechanisms.

Methods And Results: Rat model of AMI was induced by ligation of the left anterior descending coronary artery. Remote cyclic compression (RCC) on forelimb was applied to AMI rats for 3 days after the operation. RCC postconditioning displayed cardioprotective effects against AMI injury by limiting infarct size, alleviating cardiac dysfunction, and suppressing cardiomyocyte apoptosis. In addition, RCC postconditioning induced myocardial autophagy as evidenced by increased LC3-II and Beclin-1 and reduced mTOR levels. Furthermore, RCC treatment upregulated AMPK phosphorylation in the context of AMI hearts. AMPK inhibitor Compound C administration markedly abrogated RCC-mediated cardioprotective effect, as evidenced by decreased infarct size and cardiac function.

Conclusion: Our results indicated that RCC postconditioning could attenuate AMI injury through inhibiting apoptosis and promoting autophagy via AMPK signaling pathway. The research provided a novel perspective for studying the cardioprotection of RIC and possible therapeutic strategy for managing AMI injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mvr.2022.104313DOI Listing
January 2022

Pelvic floor anatomical variations in children with exstrophy-epispadias complex using MRI.

Urology 2022 Jan 14. Epub 2022 Jan 14.

Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Objective: To quantitatively measure the anatomical variations of the pelvic floor in children with exstrophy-epispadias complex (EEC) using magnetic resonance imaging (MRI).

Materials And Methods: Six cases of classic bladder exstrophy (CBE), 5 cases of penile epispadias (PE) and 11 cases of penopubic epispadias (PPE) were included. Another 8 cases with the testicular tumor were taken as the controls. A series of measurements obtained from the pelvic floor MRI were analyzed, and the measurements with significant differences were obtained by ANOVA.

Results: The pelvic floor of the CBE was significantly different from that of controls in measurements including wider pubic diastasis (p<0.001), greater posterior anal distance (p=0.019), greater posterior bladder neck distance (p=0.004), larger iliac wing angle (p<0.001), diminutive ischial angle (p<0.001), bigger puborectalis angle (p<0.001), larger ileococcygeous angle (p=0.002) and shortened anterior corporal length (p<0.001). For the PE, the posterior bladder neck distance (p=0.038) was greater than that of controls. In the PPE, the posterior bladder neck distance (p=0.001) and puborectalis angle (p=0.026) was greater than that of controls, respectively.

Conclusions: CBE shows severe anatomical variations of the pelvic floor. The bladder neck moves more anteriorly both in PE and PPE than the control. The enlarged puborectalis angle resulting from wider pubic diastasis and more anterior position of the anorectal canal is also noticed in PPE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.urology.2022.01.008DOI Listing
January 2022

Influence of citral on acrylamide formation in model systems.

Food Chem 2022 Jan 8;378:132097. Epub 2022 Jan 8.

College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Storage and Processing of Fruits and Vegetables, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, China. Electronic address:

This work aimed to evaluate the role of citral (Cit) in the formation of acrylamide (AA) in model systems. The asparagine (Asn)/glucose (Glc), Asn/Glc/ginger essential oil (GEO), and Asn/Glc/Cit model systems were prepared and analysed by UPLC-MS/MS. Cit was implicated to be a major product that contributed to the enhancement of AA formation by GEO. The addition of Cit significantly enhanced the formation of AA in the Asn/Glc model system in a dose-dependent manner. Further analysis showed Cit rather than its oxidation product played a major role in AA formation. Cit not only directly reacted with Asn via the Maillard reaction producing AA but also promoted the formation of AA between Asn and Glc.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.132097DOI Listing
January 2022

Efficacy and safety of nitrate supplementation on exercise tolerance in chronic obstructive pulmonary disease: A systematic review and meta-analysis.

Medicine (Baltimore) 2022 Jan;101(2):e28578

Pulmonary and Critical Care Medicine Department, Hangzhou Hospital of Traditional Chinese Medicine (Dingqiao District), Hangzhou, China.

Background: Exercise intolerance was prevalent in people with chronic obstructive pulmonary disease (COPD) and had a detrimental effect on the quality of life. We aimed to evaluate the efficacy and safety of nitrate supplementation in exercise tolerance of people with COPD.

Methods: We searched medical databases including Cochrane Library, EMBASE, and PubMed from inception to October 2020 for randomized control trials in treating COPD with nitrate supplementation.

Results: Nine trials were identified. Compared with placebo, nitrate supplementation has no significant effect on the following variables: exercise endurance time (standard mean difference [SMD]: 0.06; 95% confidence interval [CI]: -0.39 to 0.52; P = .79), exercise capacity (SMD: 0.30; 95% CI: -0.21 to 0.80; P = .25), oxygen consumption (SMD: -0.04; 95% CI: -0.33 to 0.25; P = .80), resting systolic blood pressure (MD: -2.84; 95% CI: -8.46 to 2.78; P = .32), systolic blood pressure after exercise (MD: -4.66; 95% CI -15.66 to 6.34; P = .41), resting diastolic blood pressure (MD: 0.89; 95% CI: -4.41 to 6.19; P = .74), diastolic blood pressure after exercise (MD: -0.21; 95% CI: -5.51 to 5.10; P = .94), heart rate (MD: -2.52; 95% CI: -7.76 to 2.73; P = .35), and arterial oxygen saturation (MD: -0.44; 95% CI: -2.38 to 1.49; P = .65). No severe adverse effects from nitrate supplementation were reported in the included trails.

Conclusion: Current evidence suggests that nitrate supplementation may be safe but ineffective for improving exercise tolerance in people with COPD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000028578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758058PMC
January 2022

Popularization of first-aid education in China: Challenges and progress.

World J Emerg Med 2022 ;13(1):65-66

Department of Emergency Medicine, Hunan Provincial Institute of Emergency Medicine, Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Hunan Provincial People's Hospital/the First Affiliated Hospital, Hunan Normal University, Changsha 410005, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5847/wjem.j.1920-8642.2022.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677913PMC
January 2022

Networked control strategy of dual linear switched reluctance motors based time delay tracking system.

ISA Trans 2021 Dec 28. Epub 2021 Dec 28.

National Taipei University of Technology, Taiwan. Electronic address:

To reduce the influence of time delay on the tracking performance of a direct-drive motion control system, this paper concentrates on stability analysis and cooperative position tracking control issues for the dual linear switched reluctance motors (LSRMs) system with network-induced time delays. The closed-loop network control system (NCS) is constructed by modeling random and bounded network-induced time delays existing in forward and feedback channels as a discrete-time Markov chain. Incremental time delay information is introduced in Lyapunov functional analysis to satisfy the high-precision movement of master and secondary motors and improve the system control performance. The time delay compensation method is proposed to compensate for the damage to the networked control system caused by random delay. With Lyapunov stability theory and LMI are applied, stability and stabilization conditions with less computational complexity and low conservatism are obtained based on incremental time delay information insertion. Finally, the numerical simulation and the experimental platform of the motor control system are built. Simulation and experiment results demonstrate that the networked control strategy can compensate the negative impact of delay on the tracking performance of LSRMs based motion control system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isatra.2021.12.034DOI Listing
December 2021

Engineering hierarchical porous ternary Co-Mn-Cu-S nanodisk arrays for ultra-high-capacity hybrid supercapacitors.

J Colloid Interface Sci 2021 Dec 28;612:298-307. Epub 2021 Dec 28.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Department of Chemistry, Zhejiang Normal University, Jinhua 321004, China; Hangzhou Institute of Advanced Studies, Zhejiang Normal University, Hangzhou 311231, China. Electronic address:

Transition-metal sulfides have been recognized as one of the promising electrodes for high-performance hybrid supercapacitors (HSCs). However, the poor rate performance and short cycle life heavily impede their practical applications. Herein, an advanced electrode based on hierarchical porous cobalt-manganese-copper sulfide nanodisk arrays (Co-Mn-Cu-S HPNDAs) on Ni foam is fabricated for high-capacity HSCs, using metal-organic frameworks as the self-sacrificial template. The synergistic effects of ternary Co-Mn-Cu sulfides and the hierarchical porous structure endow the as-obtained electrode with fast redox reaction kinetics. As expected, the resultant Co-Mn-Cu-S HPNDAs electrode delivers an ultrahigh specific capacity of 536.8 mAh g (3865 F g) at 2 A g with a superb rate performance of 63% capacity retention at 30 A g. Remarkably, an energy density of 63.8 W h kg at a power density of 743 W kg with a long cycle life is also achieved with the quasi-solid-state Co-Mn-Cu-S HPNDAs//ZIF-8-derived carbon HSC. This work offers a new pathway to fabricate high-performance multiple transition-metal-sulfide-based electrode materials for energy storage devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.12.159DOI Listing
December 2021

Dataset-driven Unsupervised Object Discovery for Region-based Instance Image Retrieval.

IEEE Trans Pattern Anal Mach Intell 2022 Jan 7;PP. Epub 2022 Jan 7.

Instance image retrieval could greatly benefit from discovering objects in the image dataset. This not only helps produce more reliable feature representation but also better informs users by delineating query-matched object regions. However, object classes are usually not predefined in a retrieval dataset and class label information is generally unavailable in image retrieval. This situation makes object discovery a challenging task. To address this, we propose a novel dataset-driven unsupervised object discovery framework. By utilizing deep feature representation and weakly-supervised object detection, we explore supervisory information from within an image dataset, construct class-wise object detectors, and assign multiple detectors to each image for detection. To efficiently construct object detectors for large image datasets, we propose a novel '`base-detector repository'' and derive a fast way to generate the base detectors. In addition, the whole framework is designed to work in a self-boosting manner to iteratively refine object discovery. Compared with existing unsupervised object detection methods, our framework produces more accurate object discovery results. Different from supervised detection, we need neither manual annotation nor auxiliary datasets to train object detectors. Experimental study demonstrates the effectiveness of the proposed framework and the improved performance for region-based instance image retrieval.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2022.3141433DOI Listing
January 2022

Ceftazidime-Avibactam as Salvage Treatment for Infections Due to Carbapenem-Resistant in Liver Transplantation Recipients.

Infect Drug Resist 2021 21;14:5603-5612. Epub 2021 Dec 21.

Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Background: Ceftazidime-avibactam (CZA) has been approved in vitro activity against carbapenem-resistant (CRKP), but the experience for the treatment of CRKP in liver transplantation (LT) recipients was limited, and previous data on its efficacy in this setting are lacking.

Methods: LT recipients with CRKP infection who received CZA treatment were reviewed retrospectively, microbiological and clinical response, adverse events were also assessed. The primary outcome was 30-day mortality after CZA administration.

Results: CZA was used in 21 LT recipients (including 4 pediatric patients) with CRKP infections after failure with other antimicrobials. CZA was administered as monotherapy in 4 patients. Median time from the onset of CRKP infection until the initiation of CZA treatment was 2 days (IQR, 1-6.5), and the median treatment duration was 12 days (IQR, 8.5-18.5). The mortality at 14 days, 30 days and all-cause was 28.6%, 38.1% and 42.9%, respectively. In adult patients, clinical response of 14 days and 30 days was 70.6% (12/17) and 58.8% (10/17), respectively, while in pediatric patients the 14-day and 30-day clinical response were both 75%, respectively. The relapse rate during the treatment developed in 3 patients after 30 days with the cessation of CZA monotherapy. CZA resistance was not detected in any case and 3 (3/21, 14.3%) patients developed acute kidney injury related to uncontrolled infection.

Conclusion: CZA shows promising results, even in monotherapy, for the treatment of patients with severe infections due to carbapenem-resistant among LT recipients. The emergence of resistance to CZA was not observed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S342163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710070PMC
December 2021

The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.

Exp Cell Res 2022 Jan 4;412(1):113009. Epub 2022 Jan 4.

Department of Neurosurgery, The First People(')s Hospital of LongQuanYi District, Chengdu, Sichuan, 610100, China. Electronic address:

LINC010503 is a novel oncogenic lncRNA in multiple cancers. In this study, we further explored the expression of LINC010503 transcripts and their regulations on the glioblastoma (GBM) stem cell (GSC) properties. LINC01503 transcription patterns in GBM and normal brain tissues were compared using RNA-seq data from Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA)-GBM. GBM cell lines (U251 and U87) were used as in vitro cell models for cellular and molecular studies. The results showed that ENST00000444125 was the dominant transcript of LINC01503 in both normal and tumor tissues. Its expression was significantly elevated in the tumor group and associated with poor survival outcomes. LINC01503 had both cytoplasmic and nuclear distribution. It positively modulated the expression of multiple GSC markers, including CD133, SOX2, NESTIN, ALDH1A1, and MSI1, and tumorsphere formation in U251 and U87 cells. RNA pull-down and RIP-qPCR assay confirmed an interaction between ENST00000444125 and GLI2. ENST00000444125 positively regulated the half-life of the GLI2 protein in GBM cells. ENST00000444125 overexpression reduced GLI2 ubiquitination and partially attenuated FBXW1 overexpression induced GLI2 ubiquitination. ENST00000444125 overexpression could activate Wnt/β-catenin signaling in GBM cells. However, these activating effects were remarkedly hampered when GLI2 was knocked down. In conclusion, this study revealed that LINC01503 might have isoform-specific dysregulation in GBM. Among the two major transcripts expressed in GBM cells, ENST00000444125 might be the major functional transcript. Its upregulation might enhance the GSC properties of GBM cells via reducing FBXW1-mediated proteasomal degradation of GLI2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yexcr.2022.113009DOI Listing
January 2022

Recent Applications of Langmuir-Blodgett Technique in Battery Research.

ACS Appl Mater Interfaces 2022 Jan 5;14(2):2431-2439. Epub 2022 Jan 5.

Energy Storage and Distributed Resources Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

The Langmuir-Blodgett (LB) technique, in which monolayers are commonly transferred from a liquid/gas interface to a solid surface, allows convenient fabrication of highly ordered thin films with molecular-level precision. This method is widely applicable to substances ranging from organic molecules to nanomaterials. Therefore, LB methods have provided a critical toolbox for researchers to engineer nanoarchitectures. The LB fabrication process is also compatible with numerous substrate materials over large areas, which is advantageous for practical application. Despite its wide applicability, the LB strategy has not been extensively employed in battery studies. The versatility of LB film, along with the accumulated knowledge associated with this technique, makes it a promising platform for promoting battery chemistry evolution. This Review summarizes recent advances of LB methods for high-performance battery development, including preparation of electrode materials, fabrication of functional layers, and battery diagnosis and thus illustrates the high utility of LB approaches in battery research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c19064DOI Listing
January 2022

Function-specific projections from V2 to V4 in macaques.

Brain Struct Funct 2022 Jan 3. Epub 2022 Jan 3.

State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, No. 19 Xin Jie Kou Wai Street, Beijing, 100875, China.

Previous studies have revealed modular projections from area V2 to area V4 in macaques. Specifically, V2 neurons in cytochrome oxidase (CO)-rich thin and CO-sparse pale stripes project to distinct regions in V4. However, how these modular projections relate to the functional subcompartments of V4 remains unclear. In this study, we injected retrograde fluorescent tracers into V4 regions with different functional properties (color, orientation, and direction) that were identified with intrinsic signal optical imaging (ISOI). We examined the labeled neurons in area V2 and their locations with respect to the CO patterns. Covariation was observed between the functional properties of the V4 injection sites and the numbers of labeled neurons in particular CO stripes. This covariation indicates that the color domains in V4 mainly received inputs from thin stripes in V2, whereas V4 orientation domains received inputs from pale stripes. Although motion-sensitive domains are present in both V2 and V4, our results did not reveal a functional specific feedforward projection between them. These results confirmed previous findings of modular projections from V2 to V4 and provided functional specificity for these anatomical projections. Together, these findings indicate that color and form remain separate in ventral mid-level visual processing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00429-021-02440-3DOI Listing
January 2022

The efficacy and safety of pyrotinib in treating HER2-positive breast cancer patients with brain metastasis: A multicenter study.

Cancer Med 2021 Dec 27. Epub 2021 Dec 27.

Department of Radiation Therapy, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Purpose: To investigate the efficacy and safety of pyrotinib in treating patients with human epidermal growth factor receptor type 2 (HER2)-positive breast cancers with brain metastasis.

Patients And Methods: This is a multicenter retrospective study, and the HER2-positive breast cancer patients with brain metastasis were studied. The enrolled patients were given pyrotinib 400 mg orally once per day for 21 days as one cycle, and evaluated every two cycles. All relevant data were detected for final assessments including medical history, clinical examination, histopathology, immunohistochemistry, radiographic imaging, treatment outcome, and adverse events.

Results: Forty-two female patients in total were enrolled in this study. The objective response rate (ORR) and disease control rate (DCR) of central nervous system (CNS), were found in 20 of 42 (47.6%) and in 39 of 42 (92.8%), respectively, while for extra-CNS, the respective ORR and DCR were in 9 of 38 (23.6%) and in 36 of 38 (94.7%), respectively. The compounded ORR and DCR were seen in 17 of 42 (40.4%) and in 39 of 42 (92.8%), respectively. The improvement rate of craniocerebral symptoms after treatment was (19/19) 100% and the median duration was 15 months. The median effective time of brain metastases and other metastases was 43 and 50 days. The median follow-up time was 22 months (interquartile range, 16.0-24.3 months). The median time for progression in brain metastasis was 16.6 months. The median time to progress for our group patients was 11.1 months. Sixteen patients (36%) with adverse reactions were recorded in the study.

Conclusion: Pyrotinib combined with chemotherapy/radiotherapy or alone showed significantly greater local control rates and progression free survival (PFS), with manageable toxicity for patients with HER2-positive breast cancer with brain metastases, and further follow-up will provide an overall survival (OS) data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cam4.4481DOI Listing
December 2021

The in-vitro digestion behaviors of micellar casein acting as wall materials in spray-dried microparticles: The relationships between colloidal calcium phosphate and the release of loaded blueberry anthocyanins.

Food Chem 2022 May 16;375:131864. Epub 2021 Dec 16.

College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, China Agricultural University, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing 100083, China. Electronic address:

Micellar casein (MC) is a natural carrier for delivering various bioactive substances, and its gastrointestinal digestion behavior has an important impact on the loaded materials. Studies have shown that the digestion behavior of MC is dominated by colloidal calcium phosphate (CCP) in micelle structure. In this paper, The MCs with different CCP levels were used as the carriers to prepare spray-dried microparticles loaded with blueberry anthocyanins (ACNs), then the release of ACNs during digestion was investigated. The results found that the microparticles with less CCP showed the faster dissolution and quicker protein hydrolysis, which caused weaker curd ability. The coagulation was believed as the critical issue to influence the digestion and release behaviors. Therefore, lowering CCP resulted in significantly more ACNs released. This study demonstrated the possibility of using CCP levels to control MC digestion behaviors, which can further determine the release of loaded bioactive substances in casein-based delivery systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.131864DOI Listing
May 2022

Simultaneous quantitation of acrylamide, 5-hydroxymethylfurfural, and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine using UPLC-MS/MS.

Food Chem 2022 May 1;375:131726. Epub 2021 Dec 1.

College of Food Science & Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, China. Electronic address:

A robust and sensitive isotope dilution UPLC-MS/MS method was established for the simultaneous analysis of acrylamide (AA), 5-hydroxymethylfurfural (HMF), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine (PhIP). A fairly good separation of three analytes was achieved within 4.5 min. High correlation coefficients (R > 0.9998) of the three compounds were obtained in their respective linear ranges. This method demonstrated low limits of detection (1.57 μg/L for AA, 0.61 μg/L for HMF, and 0.02 μg/L for PhIP) and limits of quantification (5.22 μg/L for AA, 2.03 μg/L for HMF, and 0.05 μg/L for PhIP). This method also demonstrated excellent quantification accuracy (99.02%-101.12%), precision (RSD < 6%), and recovery (82.83-119.92%) in the Maillard model systems and deep-fried meatballs. This work develops a fundamental method for the rapid simultaneous determination of AA, HMF, and PhIP in thermally processed foods that are both carbohydrates-rich and protein-rich, meanwhile providing technical support for the generation mechanism of various hazards.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.131726DOI Listing
May 2022

TypeSeg: A type-aware encoder-decoder network for multi-type ultrasound images co-segmentation.

Comput Methods Programs Biomed 2022 Feb 17;214:106580. Epub 2021 Dec 17.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, China.

Purpose: As a portable and radiation-free imaging modality, ultrasound can be easily used to image various types of tissue structures. It is important to develop a method which supports the multi-type ultrasound images co-segmentation. However, state-of-the-art ultrasound segmentation methods commonly only focus on the single type images or ignore the type-aware information.

Methods: To solve the above problem, this work proposes a novel type-aware encoder-decoder network (TypeSeg) for the multi-type ultrasound images co-segmentation. First, we develop a type-aware metric learning module to find an optimum latent feature space where the ultrasound images of the same types are close and that of the different types are separated by a certain margin. Second, depending on the extracted features, a decision module decides whether the input ultrasound images have the common tissue type or not, and the encoder-decoder network produces a segmentation mask accordingly.

Results: We evaluate the performance of the proposed TypeSeg model on the ultrasound dataset that contains four types of tissues. The proposed TypeSeg model achieves the overall best results with the mean IOU score of 87.51% ± 3.93% for the multi-type ultrasound images.

Conclusion: The experimental results indicate that the proposed method outperforms all the compared state-of-the-art algorithms for the multi-type ultrasound images co-segmentation task.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cmpb.2021.106580DOI Listing
February 2022

Correlations of FUT3 gene polymorphisms with colon polyps.

Cell Cycle 2021 Dec 22:1-9. Epub 2021 Dec 22.

Department of Endoscopy Room, Yantai Traditional Chinese Medicine Hospital, Shandong, China.

Objective: To explore the relationship between fut3 gene polymorphism and colonic polyps.

Methods: Two hundred patients with colonic polyps and 200 healthy people in our hospital in recent 3 years were taken as the research objects, as the disease group and the control group, respectively. The disease group was divided into cancerous colonic polyps group (n = 50) and non-cancerous colonic polyps group (n = 150).    The peripheral blood nucleated cells of the subjects were collected and isolated. The fut3 gene polymorphism was obtained by sequencing and analyzed combined with the expression of fut3 gene and the level of tumor markers.

Results: The frequency of allele C at rs2561796 locus in the disease group was significantly higher than that in the control group ( < 0.05). The frequency of Ag genotype at rs441158 locus in the disease group was significantly higher than that in the control group, and the frequency of Ca genotype at rs2561796 locus was significantly lower than that in the control group ( < 0.05). In the disease group, the frequency of AA + Ag in the dominant model at rs441158 was significantly higher than that in the control group, and the frequency of Ca + AA in the invisible model at rs2561796 was significantly higher than that in the control group ( < 0.05). The frequency of CGC haplotype in the disease group was higher than that in the control group ( < 0.05). The linkage disequilibrium of rs441158 and rs2561796 loci of fut3 gene was high (' = 0.423). The genotype of rs372725 of fut3 gene was correlated with the expression of fut3 gene ( < 0.05). The expression of fut3 gene in patients with CC genotype was significantly higher than that in patients with other genotypes ( < 0.05).   Conclusion: fut3 gene polymorphism is associated with the susceptibility and carcinogenesis of colonic polyps.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15384101.2021.2012036DOI Listing
December 2021
-->