Publications by authors named "Chen Dong"

2,259 Publications

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Simultaneous Visualization of Dual Intercellular Signal Transductions via SERS Imaging of Membrane Proteins Dimerization on Single Cells.

ACS Nano 2022 Aug 15. Epub 2022 Aug 15.

State Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

The visualization of protein dimerization on live cells is an urgent need and of vital importance for facile monitoring the signal transduction during intercellular communication. Herein, a highly sensitive and specific SERS strategy for simultaneously imaging dual homodimerizations of membrane proteins on single live cells was proposed by networking of AuNPs-based dual-recognition probes (dual-RPs) and SERS tags via proximity ligation-assisted catalytic hairpin assembly (CHA). The dual-RPs were prepared by comodifying hairpin-structured ssDNAs H1-Met and H1-TβRII on 50 nm AuNPs and two SERS tags for membrane proteins Met and TβRII were prepared respectively by labeling their corresponding Raman molecules and hairpin-structured single-stranded DNAs H2-Met or H2-TβRII on 15 nm AuNPs. The membrane proteins were ligated proximally by specific aptamers, and the dimerizations of proteins resulted in the proximity ligation-assisted CHA-based networking of dual-RPs and SERS tags to form 15Au-50Au network nanostructures with significantly enhanced SERS effect. The SERS strategy for visualizing the membrane protein dimerization was established and the good performance on simultaneously SERS imaging dual dimerizations of membrane proteins (i.e., Met-Met and TβRII-TβRII) was confirmed. Furthermore, the membrane protein dimerization-based signaling pathways between cancer cells and stromal cells or stem cells were observed by SERS, which indicates that the proposed SERS strategy is a good method for high-sensitivity monitoring of membrane proteins dimerizations-based multiple intercellular signal transductions in a natural and complex cellular microenvironment.
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http://dx.doi.org/10.1021/acsnano.2c03914DOI Listing
August 2022

miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3.

J Oncol 2022 2;2022:8697676. Epub 2022 Aug 2.

Department of Neurosurgery, Shenzhen People's Hospital, Shenzhen, Guangdong, China.

Objective: This research aims to investigate the expression of miR-133a-5p in glioma tissues and its impact on glioma cell proliferation.

Methods: Fluorescence-quantitative PCR was used to detect the expression of miR-133a-5p in 25 cases of glioma and adjuncent tissues. CCK-8 and colony formation analyses were used to evaluate the impact of transfection with miR-133a-5p inhibitors or mimics on glioma cell growth and colony formation. The IGFBP3 (insulin-like growth factor-binding protein-3) and miR-133a-5p binding sites were predicted using Starbase, and the miR-133a-5p binding capacity with 3'UTR of IGFBP3 gene was determined using a luciferase gene reporter system. Following transfection with miR-133a-5p mimics or inhibitors, the IGFBP3 protein expression in glioma cells was determined by western blotting. The colony formation assay was applied to evaluate the influence of IGFBP3 overexpression on the miR-133a-5p in glioma cell proliferation. For assessment of the IGFBP3 expression in glioma tissues and prognosis, TCGA database was employed.

Results: The expression of miR-133a-5p was considerably reduced in glioma tissue compared to adjuncent control tissue. In addition, miR-133a-5p expression decreased with increasing glioma malignancy. Glioma cell growth and colony formation were reduced after miR-133a-5p mimics were transfected, while transfection of miR-133a-5p inhibitors had a reverse impact. The expression of IGFBP3 was affected by miR-133a-5p by binding to its 3'UTR region. Additional study demonstrated that the overall survival (OS) of subjects with increased IGFBP3 expression was considerably lower compared to patients with decreased IGFBP3 expression. The IGFBP3 overexpression effectively counteracts the glioma cell proliferation-inhibiting impact of miR-133a-5p.

Conclusion: miR-133a-5p acts as a glioma tumor suppressor gene. It reduces glioma cell proliferation by modulating IGFBP3 and could be a target for glioma therapy.
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http://dx.doi.org/10.1155/2022/8697676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363926PMC
August 2022

Super-assembly of integrated gold magnetic assay with loop-mediated isothermal amplification for point-of-care testing.

Nano Res 2022 Aug 4:1-10. Epub 2022 Aug 4.

The College of life science, Northwest University, Xi'an, 710069 China.

With the increasing global threat of various diseases and infections, it is essential to develop a fast, low-cost, and easy-to-use point-of-care testing (POCT) system for inspections at all levels of medical institutions and self-examination at home. In this work, gold magnetic nanoparticles (GMNPs) are used as the key material, and a rapid visual detection method is designed through integrating loop-mediated isothermal amplification (LAMP) and lateral flow assay (LFA) biosensor for detecting a variety of analytes which includes whole blood, buccal swabs, and DNA. It is worth to note that the proposed method does not need DNA extraction. Furthermore, uracil DNA glycosylase (UDG) is employed to eliminate carrier contamination for preventing false positive results. The whole detection process can be finished within 25 min. The accuracy of detection is measured by assessing the polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) C677T. The detection limit of the newly developed extraction-free detection system for MTHFR C677T is 0.16 ng/μL. A preliminary clinical study of the proposed method is carried out by analyzing 600 clinical samples (including 200 whole blood samples, 100 buccal swabs, and 300 genomic DNA samples). The results indicate that the proposed method is 100% consistent with the sequencing results which provides a new choice for POCT and shows a broad application prospect in all levels of medical clinics and at home.

Electronic Supplementary Material: Supplementary material (details for MTHFR C677T primer sequences, the cell count results of samples at different dilution ratios, genotyping results and frequency samples, a Hardy-Weinberg equilibrium test, the sensitivity of the system, detection results of multiple samples, and optimization of the system) is available in the online version of this article at 10.1007/s12274-022-4692-9.
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http://dx.doi.org/10.1007/s12274-022-4692-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362447PMC
August 2022

Scalable purification of recombinant structural proteins, hagfish intermediate filament α and ɣ, from inclusion bodies for fiber formation.

Protein Expr Purif 2022 Aug 10:106152. Epub 2022 Aug 10.

Department of Biology, Utah State University, Logan, UT, 84322, USA; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA. Electronic address:

The purpose of this study was to determine a method to purify recombinant hagfish intermediate filament proteins, alpha and gamma, in a scalable manner. The study succeeded by having an increase in protein recovery of up to 35% when comparing centrifuge purification and the developed tangential flow purification. The proteins were approximately the same purity of 70% pure but further purification increased the purity of the proteins by 16%, based on ImageJ analysis. The developed tangential flow filtration purification and final purification methods could be easily scaled up to meet industry scale purification needs. The scaled-up processes described in this study did not interfere with fiber production or formation, indicating the methods can produce usable proteins for material development.
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http://dx.doi.org/10.1016/j.pep.2022.106152DOI Listing
August 2022

Ultra-Large Von Willebrand Factor and Platelets: Missing Links to the Pathogenesis of Aortic Valve Stenosis?

Authors:
Dong Chen

JACC Basic Transl Sci 2022 Jul 25;7(7):656-657. Epub 2022 Jul 25.

Division of Hematopathology, Mayo Clinic, Rochester, Minnesota, USA.

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http://dx.doi.org/10.1016/j.jacbts.2022.04.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357568PMC
July 2022

Antimicrobial Nonwoven Fabrics Incorporated with Levulinic Acid and Sodium Dodecyl Sulfate for Use in the Food Industry.

Foods 2022 Aug 7;11(15). Epub 2022 Aug 7.

College of Food Science, Southwest University, 2 Tiansheng Rd, Beibei, Chongqing 400715, China.

Safe and cost-effective antimicrobial fabrics (e.g., face masks and air filters) are conducive to preventing the spread and transmission of respiratory microorganisms in food processing plants and retail establishments. The objective of this study was to coat fabrics with two commonly used compounds in the food industry: levulinic acid (LVA) and sodium dodecyl sulfate (SDS) and determine the antimicrobial efficacy of the coated fabrics against bacterial solutions, aerosols, and influenza A virus subtype H1N1. In addition, air permeability and shelf-life of the LVA/SDS coated fabrics were also examined. Nonwoven fabrics were dip-coated with three concentrations (/, 0.5% LVA + 0.1% SDS, 1% LVA + 0.5% SDS, and 2% LVA + 1% SDS) of LVA and SDS and challenged with bacterial solutions ( and , ca. 7.0 log CFU/coupon) for a contact time of 3, 5, and 10 min. The coated fabrics were also challenged with aerosol and H1N1 virus following standard operations of ASTM F2101-19 and ISO 18184:2019, respectively. The 1% LVA + 0.5% SDS coated fabrics showed potent antibacterial efficacy against both bacterial solutions (>6.0-log reduction to under the detection limit of 1.0 log CFU/coupon for ; ca. 1.0-log reduction for ) and aerosols (>3.6-log reduction to under the detection limit), with greater inactivation occurring at higher concentrations and longer exposure time. Moreover, the coated fabrics inactivated >99% of the H1N1 virus. The shelf-life of the coated fabrics was stable within 12 months and the air permeability was not adversely affected with the coating concentrations less than 1% LVA + 0.5% SDS. Results reveal these low-cost and safe materials have the potential to be used to coat fabrics in the food industry to combat the spread and transmission of pathogens.
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http://dx.doi.org/10.3390/foods11152369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368506PMC
August 2022

In special coagulation, think horses not zebras: A clotted sample from a patient with congenital disorder of glycosylation.

Int J Lab Hematol 2022 Aug 10. Epub 2022 Aug 10.

Department of Laboratory Medicine And Pathology, Mayo Clinic, Rochester, Minnesota, USA.

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http://dx.doi.org/10.1111/ijlh.13944DOI Listing
August 2022

Highly conductive, Transparent, Adhesive and Self-healable Ionogel Based on a Deep Eutectic Solvent with Widely Adjustable Mechanical Strength.

Macromol Rapid Commun 2022 Aug 10:e2200480. Epub 2022 Aug 10.

State Key Laboratory of Chemical Resource Engineering, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Ionogels have attracted intensive attentions as promising flexible conductive materials. However, simultaneous integration of excellent mechanical properties, high conductivity, outstanding self-healing ability and strong adhesiveness is still challenging. Here, an ingenious composition design is proposed to address this long-standing challenge of ionogels. High-performance PEI/PAA/CMC ionogels, consisted of a loosely cross-linked poly(acrylic acid) (PAA) network, dynamically cross-linked network based on polycationic polyethyleneimine (PEI) and polyanionic PAA, and carboxymethyl cellulose (CMC) reinforcing filler, are formed in a deep eutectic solvent (DES) composed of choline chloride and urea. Benefiting from the loose PAA network and dynamic noncovalent interactions, ionogels with both highly enhanced mechanical robustness and excellent conductivity are obtained at high loading of DES, overcoming the strength-ductility/conductivity trade-off dilemma. By adjusting PEI/PAA mass ratio, the tensile strength and strain of PEI/PAA/CMC ionogels are effectively controlled in a wide range of 0.15-7.9 MPa and 232-1161%, respectively, while maintaining the desirable conductivity of ∼10 S cm . Besides, healed tensile strength over 2.1 MPa and adhesion strength up to 0.2 MPa are achieved for the PEI /PAA /CMC ionogel. The delicate design strategy provides a feasible approach to prepare ionogels with outstanding comprehensive performance, which have potential for applications in flexible electronics. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/marc.202200480DOI Listing
August 2022

Emerging trends in sacubitril/valsartan research: A bibliometric analysis of the years 1995-2021.

Medicine (Baltimore) 2022 Aug;101(31):e29398

Department of Cardiology, The First Hospital of Gannan Medical University, Gannan Medical University, Ganzhou, China.

Background: Sacubitril/valsartan has been approved for the treatment of heart failure (HF) patients with reduced ejection fraction; since then, it gradually became a new star drug in the therapy of HF. Nevertheless, the effectiveness of sacubitril/valsartan remains under investigation. Thus far, only a few bibliometric studies have systematically analyzed the application of sacubitril/valsartan.

Methods: Publications on sacubitril/valsartan were retrieved from the Web of Science Core Collection on April 29, 2021. Data were analyzed using Microsoft Excel 2019 (Redmond, WA), VOS viewer (Redmond, WA), and Cite Space V (Drexel University, Philadelphia, PA).

Results: A total of 1309 publications on sacubitril/valsartan published from 1995 to 2021 were retrieved. The number of publications regarding sacubitril/valsartan increased sharply in the last 6 years (2015-2021), and American scholars authored >40% of those publications. Most were published in the European Journal of Heart Failure, the United States was the bellwether with a solid academic reputation in this area. Solomon published the highest number of related articles and was the most frequently cited author. "Heart failure" was the leading research hotspot. The keywords, "inflammation," "fibrosis," and "oxidative stress" appeared most recently as research fronts.

Conclusions: Research attention should be focused on clinical trial outcomes. Considering its effectiveness in HF, the mechanisms and further applications of sacubitril/valsartan may become research hotspots in the future and should be closely examined.
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http://dx.doi.org/10.1097/MD.0000000000029398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351920PMC
August 2022

RALY regulate the proliferation and expression of immune/inflammatory response genes via alternative splicing of FOS.

Genes Immun 2022 Aug 8. Epub 2022 Aug 8.

Department of Intensive Care Unit, The First Affiliated Hospital of Xinjiang Medical University, 393 South Li Yu Shan Road, Urumqi, Xinjiang, 830054, China.

RALY is a multifunctional RNA-binding protein involved in cancer metastasis, prognosis, and chemotherapy resistance in various cancers. However, the molecular mechanism of which is still unclear. We have established RALY overexpression cell lines and studied the effect of RALY on proliferation and apoptosis in HeLa cells. Then we used RNA-seq to analyze the transcriptomes data. Lastly, RT-qPCR experiments had performed to confirm the RNA-seq results. We found that the overexpression of RALY in HeLa cells inhibited proliferation. Moreover, the overexpression of RALY changed the gene expression profile, and the significant upregulation of genes involved immune/inflammatory response related biological process by NOD-like receptor signaling pathway cytokine-cytokine receptor interaction. The significant downregulation genes involved innate immune response by the Primary immunodeficiency pathway. Notably, IFIT1, IFIT2, IFTI3, IFI44, HERC4, and OASL expression had inhibited by the overexpression of RALY. Furthermore, RALY negatively regulates the expression of transcription factors FOS and FOSB. Notably, we found that 645 alternative splicing events had regulated by overexpression of RALY, which is highly enriched in transcription regulation, RNA splicing, and cell proliferation biological process by the metabolic pathway. We show that RALY regulates the expression of immune/inflammatory response-related genes via alternative splicing of FOS in HeLa cells. The novel role of RALY in regulating immune/inflammatory gene expression may explain its function in regulating chemotherapy resistance and provides novel insights into further exploring the molecular mechanism of RALY in regulating cancer immunity and chemo/immune therapies.
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http://dx.doi.org/10.1038/s41435-022-00178-4DOI Listing
August 2022

Deciphering the focuses and trends in skin regeneration research through bibliometric analyses.

Front Med (Lausanne) 2022 22;9:947649. Epub 2022 Jul 22.

Department of Oral and Maxillofacial Surgery, Xi'an Daxing Hospital, Xi'an, China.

Increasing attention to skin regeneration has rapidly broadened research on the topic. However, no bibliometric analysis of the field's research trends has yet been conducted. In response to this research gap, this study analyzed the publication patterns and progress of skin regeneration research worldwide using a bibliometric analysis of 1,471 papers comprising 1,227 (83.4%) original articles and 244 (16.6%) reviews sourced from a Web of Science search. Publication distribution was analyzed by country/region, institution, journal, and author. The frequency of keywords was assessed to prepare a bibliometric map of the development trends in skin regeneration research. China and the United States were the most productive countries in the field: China had the greatest number of publications at 433 (29.4%) and the United States had the highest H-index ranking (59 with 15,373 citations or 31.9%). Author keywords were classified into four clusters: stem cell, biomaterial, tissue engineering, and wound dressing. "Stem cells," "chitosan," "tissue engineering," and "wound dressings" were the most frequent keywords in each cluster; therefore, they reflected the field's current focus areas. "Immunomodulation," "aloe vera," "extracellular vesicles," "injectable hydrogel," and "three-dimensional (3D) bioprinting" were relatively new keywords, indicating that biomaterials for skin regeneration and 3D bioprinting are promising research hotspots in the field. Moreover, clinical studies on new dressings and techniques to accelerate skin regeneration deserve more attention. By uncovering current and future research hotspots, this analysis offers insights that may be useful for both new and experienced scholars striving to expand research and innovation in the field of skin regeneration.
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http://dx.doi.org/10.3389/fmed.2022.947649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355679PMC
July 2022

Exploring Different Effects of Exclusive Enteral Nutrition (EEN) and Corticosteroids on the Gut Microbiome in Crohn's Disease Based on a Three-Stage Strategy.

Gastroenterol Res Pract 2022 27;2022:6147124. Epub 2022 Jul 27.

Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China.

The objective of this study was to compare the efficacy of exclusive enteral nutrition (EEN) and corticosteroids on the gut microbiome in Crohn's disease. . Data were collected for 16 patients newly diagnosed with CD as the test group and 10 healthy volunteers as the control group. The 16 patients were randomly divided into the EEN group and the corticosteroids group. For subsequent analysis, 6 patients in the EEN group with follow-up were enrolled to compare the 0-month, 1-month, and 3-month outcomes. We analyzed and compared gut microbiota between different groups in 3 stages. To evaluate the clinical outcome of treatment, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), hemoglobin (HB), albumin (ALB), and Crohn's disease activity (CDAI) were recorded. . There are significant differences in microbiota between patients with CD and healthy people, and there are intuitive differences in the main components of the microbiota. 16 patients were included in stage 2, in which both corticosteroids and EEN can induce CD remission well. However, corticosteroids have a greater impact on inflammatory indicators, while EEN has a more obvious effect on nutritional indicators. Principal component analysis suggests that there are different compositional changes in the gut microbiome after corticosteroids and EEN treatment. After 3 months of dynamic observation, we found that EEN can effectively maintain CD remission, reduce inflammatory indicators, and improve nutritional indicators. . Both EEN and corticosteroids can increase the diversity of the microbiome in inducing CD remission, while they have different effects on the proportion of microbiome species. This trial is registered with NCT02056418.
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http://dx.doi.org/10.1155/2022/6147124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348958PMC
July 2022

In vivo processing of digital information molecularly with targeted specificity and robust reliability.

Sci Adv 2022 Aug 5;8(31):eabo7415. Epub 2022 Aug 5.

Department of Chemistry, Tsinghua University, Beijing, China.

DNA has attracted increasing interest as an appealing medium for information storage. However, target-specific rewriting of the digital data stored in intracellular DNA remains a grand challenge because the highly repetitive nature and uneven guanine-cytosine content render the encoded DNA sequences poorly compatible with endogenous ones. In this study, a dual-plasmid system based on gene editing tools was introduced into to process information accurately. Digital data containing large repeat units in binary codes, such as text, codebook, or image, were involved in the realization of target-specific rewriting in vivo, yielding up to 94% rewriting reliability. An optical reporter was introduced as an advanced tool for presenting data processing at the molecular level. Rewritten information was stored stably and amplified over hundreds of generations. Our work demonstrates a digital-to-biological information processing approach for highly efficient data storage, amplification, and rewriting, thus robustly promoting the application of DNA-based information technology.
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http://dx.doi.org/10.1126/sciadv.abo7415DOI Listing
August 2022

Early prediction of treatment response to neoadjuvant chemotherapy based on longitudinal ultrasound images of HER2-positive breast cancer patients by Siamese multi-task network: A multicentre, retrospective cohort study.

EClinicalMedicine 2022 Oct 30;52:101562. Epub 2022 Jul 30.

Department of Radiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhangshan Er Road, Guangzhou 510080, China.

Background: Early prediction of treatment response to neoadjuvant chemotherapy (NACT) in patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer can facilitate timely adjustment of treatment regimens. We aimed to develop and validate a Siamese multi-task network (SMTN) for predicting pathological complete response (pCR) based on longitudinal ultrasound images at the early stage of NACT.

Methods: In this multicentre, retrospective cohort study, a total of 393 patients with biopsy-proven HER2-positive breast cancer were retrospectively enrolled from three hospitals in china between December 16, 2013 and March 05, 2021, and allocated into a training cohort and two external validation cohorts. Patients receiving full cycles of NACT and with surgical pathological results available were eligible for inclusion. The key exclusion criteria were missing ultrasound images and/or clinicopathological characteristics. The proposed SMTN consists of two subnetworks that could be joined at multiple layers, which allowed for the integration of multi-scale features and extraction of dynamic information from longitudinal ultrasound images before and after the first /second cycles of NACT. We constructed the clinical model as a baseline using multivariable logistic regression analysis. Then the performance of SMTN was evaluated and compared with the clinical model.

Findings: The training cohort, comprising 215 patients, were selected from Yunnan Cancer Hospital. The two independent external validation cohorts, comprising 95 and 83 patients, were selected from Guangdong Provincial People's Hospital, and Shanxi Cancer Hospital, respectively. The SMTN yielded an area under the receiver operating characteristic curve (AUC) values of 0.986 (95% CI: 0.977-0.995), 0.902 (95%CI: 0.856-0.948), and 0.957 (95%CI: 0.924-0.990) in the training cohort and two external validation cohorts, respectively, which were significantly higher than that those of the clinical model (AUC: 0.524-0.588, < 0.05). The AUCs values of the SMTN within the anti-HER2 therapy subgroups were 0.833-0.972 in the two external validation cohorts. Moreover, 272 of 279 (97.5%) non-pCR patients (159 of 160 (99.4%), 53 of 54 (98.1%), and 60 of 65 (92.3%) in the training and two external validation cohorts, respectively) were successfully identified by the SMTN, suggesting that they could benefit from regime adjustment at the early-stage of NACT.

Interpretation: The SMTN was able to predict pCR in the early-stage of NACT for HER2-positive breast cancer patients, which could guide clinicians in adjusting treatment regimes.

Funding: Key-Area Research and Development Program of Guangdong Province (No.2021B0101420006); National Natural Science Foundation of China (No.82071892, 82171920); Guangdong Provincial Key Laboratory of Artificial Intelligence in Medical Image Analysis and Application (No.2022B1212010011); the National Science Foundation for Young Scientists of China (No.82102019, 82001986); Project Funded by China Postdoctoral Science Foundation (No.2020M682643); the Outstanding Youth Science Foundation of Yunnan Basic Research Project (202101AW070001); Scientific research fund project of Department of Education of Yunnan Province(2022J0249). Science and technology Projects in Guangzhou (202201020001;202201010513); High-level Hospital Construction Project (DFJH201805, DFJHBF202105).
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http://dx.doi.org/10.1016/j.eclinm.2022.101562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343415PMC
October 2022

Study of the microstructure of chitosan aerogel beads prepared by supercritical CO drying and the effect of long-term storage.

RSC Adv 2022 Jul 21;12(33):21041-21049. Epub 2022 Jul 21.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 PR China

In order to investigate the pore properties and effect of storage time on the microstructure of CO-dried aerogels, chitosan aerogel beads were obtained from chitosan hydrogels with an initial concentration in the range of 1.5-3.0 wt% through SCCO drying and freeze-drying (as a comparison). The SCCO-dried chitosan aerogels showed a three-dimensional network structure, and had higher BET surface area (200 m g) and higher crystallinity (0.62/XRD, 0.80/ATR-FTIR) than the freeze-dried aerogels. The stability of the microstructure of the SCCO-dried chitosan aerogel beads during 10 months was studied. The BET surface area of the aerogel beads at each concentration declined by 30.5% at 2 months, 56.7% at 6 months and 67.2% at 10 months. Accelerated aging tests of the chitosan aerogel beads were carried out to study the effect of humidity on the chitosan aerogel beads. The average diameter of the chitosan aerogel decreased from 2.3 mm to 0.9 mm when stored at 65 °C with 90% relative humidity (RH). In contrast, there was no obvious change during storage at 65 °C with 20% RH. The amount of adsorbed water increased from 4% to 12% at 65 °C with 90% RH for 96 h, and the bound water content of the aerogel beads gradually increased. This study demonstrates that SCCO-dried chitosan aerogel beads could be better at maintaining their mesoporous structure, and the adsorption of water from the surrounding air had a significant effect on the microstructure and shrinkage of the chitosan aerogel beads.
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http://dx.doi.org/10.1039/d2ra01875fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301543PMC
July 2022

Targeting the radiation-induced ARv7-mediated circNHS/miR-512-5p/XRCC5 signaling with Quercetin increases prostate cancer radiosensitivity.

J Exp Clin Cancer Res 2022 Aug 3;41(1):235. Epub 2022 Aug 3.

George Whipple Lab for Cancer Research, Departments of Pathology, Urology, Radiation Oncology and The Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY, 14642, USA.

Background: Radiation therapy (RT) with androgen deprivation therapy (ADT) is an effective therapy to suppress the locally advanced prostate cancer (PCa). However, we unexpectedly found that RT could also induce the androgen receptor splice variant 7 (ARv7) expression to decrease the radiosensitivity.

Methods: The study was designed to target ARv7 expression with Quercetin or ARv7-shRNA that leads to enhancing and increasing the radiation sensitivity to better suppress the PCa that involved the modulation of the circNHS/miR-512-5p/XRCC5 signaling.

Results: Mechanism studies revealed that RT-induced ARv7 may function via altering the circNHS/miR-512-5p/XRCC5 signaling to decrease the radiosensitivity. Results from preclinical studies using multiple in vitro cell lines and in vivo mouse models concluded that combining RT with the small molecule of Quercetin to target full-length AR and ARv7 could lead to better efficacy to suppress PCa progression.

Conclusion: Together, these results suggest that ARv7 may play key roles to alter the PCa radiosensitivity, and targeting this newly identified ARv7 mediated circNHS/miR-512-5p/XRCC5 signaling with Quercetin may help physicians to develop a novel RT to better suppress the progression of PCa.
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http://dx.doi.org/10.1186/s13046-022-02287-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347162PMC
August 2022

RNA-binding protein CELF6 modulates transcription and splicing levels of genes associated with tumorigenesis in lung cancer A549 cells.

PeerJ 2022 26;10:e13800. Epub 2022 Jul 26.

Wuhan Ruixing Biotechnology Co. Ltd., Wuhan, Hubei Province, China.

CELF6 (CUGBP Elav-Like Family Member 6), a canonical RNA binding protein (RBP), plays important roles in post-transcriptional regulation of pre-mRNAs. However, the underlying mechanism of lower expressed CELF6 in lung cancer tissues is still unclear. In this study, we increased CELF6 manually in lung cancer cell line (A549) and utilized transcriptome sequencing (RNA-seq) technology to screen out differentially expressed genes (DEGs) and alternative splicing events (ASEs) after CELF6 over-expression (CELF6-OE). We found that CELF6-OE induced 417 up-regulated and 1,351 down-regulated DEGs. Functional analysis of down-regulated DEGs showed that they were highly enriched in immune/inflammation response- related pathways and cell adhesion molecules (CAMs). We also found that CELF6 inhibited the expression of many immune-related genes, including TNFSF10, CCL5, JUNB, BIRC3, MLKL, PIK3R2, CCL20, STAT1, MYD88, and CFS1, which mainly promote tumorigenesis in lung cancer. The dysregulated DEGs were also validated by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) experiment. In addition, CELF6 regulates the splicing pattern of large number of genes that are enriched in p53 signaling pathway and apoptosis, including TP53 and CD44. In summary, we made an extensive analysis of the transcriptome profile of gene expression and alternative splicing by CELF6-OE, providing a global understanding of the target genes and underlying regulation mechanisms mediated by CELF6 in the pathogenesis and development of lung cancer.
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http://dx.doi.org/10.7717/peerj.13800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336609PMC
July 2022

SMAD4, activated by the TCR-triggered MEK/ERK signaling pathway, critically regulates CD8 T cell cytotoxic function.

Sci Adv 2022 07 27;8(30):eabo4577. Epub 2022 Jul 27.

Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China.

Transforming growth factor-β is well known to restrain cytotoxic T cell responses to maintain self-tolerance and to promote tumor immune evasion. In this study, we have investigated the role of SMAD4, a core component in the TGF-β signaling pathway, in CD8 T cells. Unexpectedly, we found that SMAD4 was critical in promoting CD8 T cell function in both tumor and infection models. SMAD4-mediated transcriptional regulation of CD8 T cell activation and cytotoxicity was dependent on the T cell receptor (TCR) but not TGF-β signaling pathway. Following TCR activation, SMAD4 translocated into the nucleus, up-regulated genes encoding TCR signaling components and cytotoxic molecules in CD8 T cells and thus reinforced T cell function. Biochemically, SMAD4 was directly phosphorylated by ERK at Ser residue following TCR activation. Our study thus demonstrates a critical yet unexpected role of SMAD4 in promoting CD8 T cell-mediated cytotoxic immunity.
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http://dx.doi.org/10.1126/sciadv.abo4577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328680PMC
July 2022

One-Step Synthesis of Nitrogen/Fluorine Co-Doped Carbon Dots for Use in Ferric Ions and Ascorbic Acid Detection.

Nanomaterials (Basel) 2022 Jul 12;12(14). Epub 2022 Jul 12.

Cixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, CAS Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.

Carbon dots (CDs) have caught enormous attention owing to their distinctive properties, such as their high water solubility, tunable optical properties, and easy surface modification, which can be generally used for the detection of heavy metals and organic pollutants. Herein, nitrogen and fluorine co-doped carbon dots (NFCDs) were designed via a rapid, low-cost, and one-step microwave-assisted technique using DL-malic acid and levofloxacin. The NFCDs emitted intense green fluorescence under UV lighting, and the optical emission peak at 490 nm was observed upon a 280 nm excitation, with a high quantum yield of 21.03%. Interestingly, the spectral measurements illustrated excitation-independent and concentration-independent single-color fluorescence owing to the presence of nitrogen and fluorine elements in the surface functional groups. Additionally, the NFCDs were applied for the selective detection of Fe and ascorbic acid based on the "turn-off" mode. The detection limits were determined as 1.03 and 4.22 µM, respectively. The quenching mechanisms were explored using the static quenching mechanism and the inner filter effect. Therefore, a NFCDs fluorescent probe with single color emission was successfully developed for the convenient and rapid detection of Fe and ascorbic acid in environments.
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http://dx.doi.org/10.3390/nano12142377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323265PMC
July 2022

Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus.

Molecules 2022 Jul 19;27(14). Epub 2022 Jul 19.

College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.

As the largest group of structurally diverse metabolites, terpenoids are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. However, few terpenoid compounds have been identified in plant parts of sacred lotus ( Gaertn.). To elucidate the molecular genetic basis of the terpene biosynthetic pathway, terpenes from different parts of the plant, including seeds (S), young leaves (YL), mature leaves (ML), white flowers (WF), yellow flowers (YF), and red flowers (RF), were identified by LC-MS/MS and the relative contents of the same terpenes in different parts were compared. The results indicate that all plant parts primarily consist of triterpenes, with only minor quantities of sesquiterpenes and diterpenes, and there were differences in the terpene content detected in different plant parts. To illustrate the biosynthesis of various terpenoids, RNA sequencing was performed to profile the transcriptomes of various plant parts, which generated a total of 126.95 GB clean data and assembled into 29,630 unigenes. Among these unigenes, 105 candidate unigenes are involved in the mevalonate (MVA) pathway, methyl-erythritol phosphate (MEP) pathway, terpenoid backbone biosynthesis pathway, and terpenoid synthases pathway. Moreover, the co-expression network between terpene synthase (TPS) and WRKY transcription factors provides new information for the terpene biosynthesis pathway.
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http://dx.doi.org/10.3390/molecules27144599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320166PMC
July 2022

NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in .

Genes (Basel) 2022 Jun 24;13(7). Epub 2022 Jun 24.

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.

As one of the most imperative antioxidants in higher plants, carotenoids serve as accessory pigments to harvest light for photosynthesis as well as photoprotectors for plants to adapt to high light stress. Phytoene synthase (PSY) is the entry enzyme and also the major rate-limiting enzyme in the carotenoid pathway. Here, we report a dehydration-responsive element-binding protein (DREB) transcription factor member in K326, NtDREB-1BL1, which regulates carotenoids biosynthesis by binding to the promoter. The transcript was widely distributed in leaves by Real-time PCR. Confocal image revealed that NtDREB-1BL1 was localized in the nucleus. The chromatin immunoprecipitation (ChIP) with the qPCR technique indicated that NtDREB-1BL1 could anchor the promoter region of . Overexpression () and RNA interference () of were performed to evaluate its biological function in . Both carotenoid and chlorophyll contents increased in transgenic plants of compared with wild-type (WT) plants, with the augment of the genes involved in carotenoid biosynthesis. In contrast, the contents of carotenoid and chlorophyll significantly decreased in transgenic plants of compared to WT, along with the decline in the expression of genes related to carotenoid biosynthesis. Moreover, transgenic plants of exhibited enhanced tolerance under drought stress, with the weakened tolerance of drought stress in transgenic plants of . In conclusion, our results illustrated the new role of transcription factor NtDREB-1BL1 in improving carotenoid biosynthesis through regulating expression.
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http://dx.doi.org/10.3390/genes13071134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322988PMC
June 2022

Primary Tumor Resection Provides Survival Benefits for Patients with Synchronous Brain Metastases from Colorectal Cancer.

Diagnostics (Basel) 2022 Jun 29;12(7). Epub 2022 Jun 29.

Department of Radiation Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

Background: Brain metastases (BMs), particularly synchronous brain metastases, in colorectal cancer (CRC) patients are uncommon. The survival benefit of primary tumor resection (PTR) in patients with metastatic colorectal cancer is controversial. Whether PTR can bring survival benefits to patients with BMs of CRC has not been reported.

Methods: From 2010 to 2016, 581 CRC patients with BMs from the Surveillance, Epidemiology, and End Results (SEER) database were divided into PTR and non-PTR groups. The log-rank test was used to compare the survival distributions. The Kaplan-Meier method was used to estimate survival. By controlling additional prognostic factors, a Cox proportional multivariate regression analysis was used to estimate the survival benefit of PTR.

Results: The median overall survival for CRC patients with synchronous BMs was 3 months, with a 1-year survival rate of 27.2% and a 2-year survival rate of 12.8%. The PTR group contained 171 patients (29.4%), whereas the non-PTR group had 410 patients (70.6%). Patients who underwent PTR had a 1-year survival rate of 40.2% compared to 21.7% in those who did not ( < 0.0001). Cox proportional analysis showed that patients ≥60 years (hazard ratio [HR] 1.718, 95% confidence interval [CI] 1.423-2.075, < 0.0001) had a shorter OS than patients < 60 years of age. OS was better in CEA-negative than in CEA-positive patients (HR 0.652, 95% CI 0.472-0.899, = 0.009). Patients in whom the primary tumor was removed had considerably improved prognoses (HR 0.654, 95% CI 0.531-0.805, < 0.0001). Subgroup analysis revealed that the PTR group achieved a survival advantage except for patients with CEA negative.

Conclusions: Patients with synchronous BMs from CRC may benefit from primary tumor resection (PTR). Age, CEA level, and PTR were independent prognostic risk factors for CRC patients with synchronous BMs.
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http://dx.doi.org/10.3390/diagnostics12071586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322496PMC
June 2022

Quantum Linear System Algorithm for General Matrices in System Identification.

Entropy (Basel) 2022 Jun 29;24(7). Epub 2022 Jun 29.

College of Information and Communication, National University of Defense Technology, Xi'an 710006, China.

Solving linear systems of equations is one of the most common and basic problems in classical identification systems. Given a coefficient matrix and a vector , the ultimate task is to find the solution such that Ax=b. Based on the technique of the singular value estimation, the paper proposes a modified quantum scheme to obtain the quantum state |x⟩ corresponding to the solution of the linear system of equations in O(κ2rpolylog(mn)/ϵ) time for a general m×n dimensional , which is superior to existing quantum algorithms, where κ is the condition number, is the rank of matrix and ϵ is the precision parameter. Meanwhile, we also design a quantum circuit for the homogeneous linear equations and achieve an exponential improvement. The coefficient matrix in our scheme is a sparsity-independent and non-square matrix, which can be applied in more general situations. Our research provides a universal quantum linear system solver and can enrich the research scope of quantum computation.
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http://dx.doi.org/10.3390/e24070893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323527PMC
June 2022

Effects of Variability in Glycemic Indices on Longevity in Chinese Centenarians.

Front Nutr 2022 8;9:955101. Epub 2022 Jul 8.

Research Center of Gerontology and Longevity, Affiliated Hospital of Nantong University, Nantong, China.

Background: Large fluctuations in blood glucose levels greatly impact the health and life span of elderly individuals. This study describes the characteristics of variability in glycemic indices in centenarians with the aim of emphasizing the importance of glycemic variability in elderly people.

Methods: We recruited individuals from Rugao City, Jiangsu Province, China from April 2020 to May 2021. The study cohort included 60 centenarians and 60 first-generation offspring, as well as 20 randomly selected non-cohabitant control individuals aged 60-80 years. A FreeStyle Libre H (hospital version) continuous glucose monitoring (CGM) device (Abbott Ireland UK) was used to measure glycemic variability. The indices measured included the time in target glucose range (TIR), time below target glucose range (TBR), time above target glucose range (TAR), mean amplitude of glycemic excursions (MAGE), mean of daily differences (MODD), coefficient of variation (CV), standard deviation of blood glucose (SDBG), continuous overlapping net glycemic action (CONGA), glucose management indicator (GMI) and estimated glycated hemoglobin (eHbA1c). Logistic regression was used to analyze the association between glycemic variability and longevity.

Results: Mean blood glucose (MBG), eHbA1c, GMI, mean fasting plasma glucose (M-FPG) and CONGA were lower in the centenarian group ( all < 0.05). PPGE-2 was higher in the control group than that measured in the centenarian and first-generation offspring groups ( < 0.05). There were no differences between the groups in MAGE, MODD, MAG, or TIR ( > 0.05). The risk of not achieving longevity increased with each one unit increase in MBG by 126% [2.26 (1.05-4.91)], eHbA1c by 67% [1.67 (1.03-2.72)], GMI by 568% [6.68 (1.11-40.30)], M-FPG by 365% [4.65 (1.57-13.75)], M-PPG1h by 98% [1.98 (1.18-3.31)], CONGA1 by 102% [2.02 (1.01-4.06)], Li by 200% [3.00 (1.04-8.61)], and PPGE-2 by 150% [2.50 (1.39-4.50)]. However, the risk of achieving longevity decreased with each unit increase of LBGI by 53% [0.47 (0.28-0.80)], ADRR by 60% [0.40 (0.18-0.86)], and TBR by 11% [0.89 (0.80-0.98)].

Conclusion: Fluctuation in blood glucose levels in centenarians is relatively small. Maintaining an average blood glucose level and keeping blood glucose fluctuations in the normal range is conducive to longevity.
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http://dx.doi.org/10.3389/fnut.2022.955101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307500PMC
July 2022

Transcriptome Analysis Reveals the Algicidal Mechanism of against through Multiple Metabolic Pathways.

Toxins (Basel) 2022 Jul 15;14(7). Epub 2022 Jul 15.

Department of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.

It is widely accepted that eutrophication has played an important role in the formation of harmful cyanobacterial blooms in recent decades, which impacts water quality and ecological environment and causes huge economic losses. Algicidal bacteria have a promising application prospect in controlling cyanobacterial blooms in aquaculture water. Here, the process of the algicidal bacterium strain Bl-zj acting on was explored using transcriptome analysis to elucidate the algicidal mechanism. The results of the co-culture of bacterium and alga showed a strong alga-lysing effect of against with an extreme morphology deformation of the algal cells. A total of 2744 differentially expressed genes of were identified, which were mainly involved in the metabolism of amino acid, carbohydrate, and lipid. In the co-cultured group, the expression of genes mainly enriched in valine, leucine and isoleucine degradation, and fatty acid degradation were significantly increased. However, the expression of the genes related to ribosome were mainly inhibited. Transcriptome analysis showed that obtained ATP and energy by the degradation of valine, leucine, isoleucine, and fatty acids, and destroyed algal cells by efflux pump transporters, secretion of hydrolytic enzymes, antibiotics, proteases, and other secondary metabolites, resulting in algal death and achieving the algicidal effect.
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http://dx.doi.org/10.3390/toxins14070492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320710PMC
July 2022

Development and validation of a nomogram for predicting mild cognitive impairment in middle-aged and elderly people.

Asian J Psychiatr 2022 Jul 19;75:103224. Epub 2022 Jul 19.

School of Public Health, Nantong University, Nantong 226001, China; Research Center of Gerontology and Longevity, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China; Nantong University Medical School, Nantong 226001, China. Electronic address:

Background: Mild cognitive impairment (MCI) is a clinical cognitive impairment state between dementia and normal aging. Early identification of MCI is beneficial, and it can delay the development of dementia. We aimed to develop and validate a prediction model to predict MCI of middle-aged and elderly people (aged 45 years and over).

Methods: According to 478 middle-aged and elderly people (48-85 years old) from a cross-sectional study, we developed and validated a predictive nomogram. The least absolute shrinkage and selection operator (LASSO) regression model and multivariate logistic regression analysis were used to select variables and develop a prediction model. The performance of the nomogram was evaluated in terms of its discriminative power, calibration, and decision curve analysis (DCA).

Results: The predictive nomogram was composed of the following: age, gender, education level, residence, and reading. The model showed good discrimination power (area under receiver-operating characteristic (ROC) curve was 0.8704) and good calibration. Similar results were seen in 10-fold cross-validation. The nomogram showed clinically useful in DCA analysis.

Conclusion: This predictive nomogram provides researchers with a practical tool for predicting MCI. The variables included in this nomogram were readily available. The population used for this nomogram was middle-aged and elderly people.
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http://dx.doi.org/10.1016/j.ajp.2022.103224DOI Listing
July 2022

Single-Atom-Kernelled Nanocluster Catalyst.

Nano Lett 2022 Jul 22. Epub 2022 Jul 22.

Key Laboratory of Materials Physics, Anhui Key Laboratory of Nanomaterials and Nanotechnology, CAS Center for Excellence in Nanoscience, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, P.R. China.

To propose the concept of single-atom-kernelled nanocluster, we synthesized a Pd-based trimetal nanocluster with a single-Ag atom-kernel for the first time by introducing some steric hindrance factors and employing a joint alloying strategy that combines the coreduction with an antigalvanic reduction (AGR). Although the AGR-derived Pd-based trimetal nanoclusters with single-silver atom kernels have low contents of gold, they show higher activity and selectivity than those of the bimetal precursor nanocluster in the electrocatalytical reduction of CO to CO. Furthermore, it is revealed that the kernel single atoms from both AuPd(TBBT) and AuAgPd(TBBT) are not the active sites for catalysis, but greatly influence the catalytical performance by effecting the electronic configuration. Thus, it is demonstrated that the single-atom-kernelled nanocluster can not only improve the precious metal utilization (even to 100%) but also better the properties and provide insight into the structure-property correlation for metal nanoclusters.
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http://dx.doi.org/10.1021/acs.nanolett.2c02290DOI Listing
July 2022

HnRNP K regulates inflammatory gene expression by mediating splicing pattern of transcriptional factors.

Exp Biol Med (Maywood) 2022 Jul 22:15353702221110649. Epub 2022 Jul 22.

Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuchang District, Hubei 430071, China.

HnRNP K is a heterogeneous nuclear ribonucleoprotein and has been identified as an oncogene in most solid tumors via regulating gene expression or alternative splicing of genes by binding both DNA and pre-mRNA. However, how hnRNP K affects tumorigenesis and regulates the gene expression in cervical cancer (CESC) remains to be elucidated. In these data, higher expression of hnRNP K was observed in CESC and was negatively correlated with the patient survival time. We then overexpressed hnRNP K (hnRNP K-OE) and found that its overexpression promoted cell proliferation in HeLa cells ( = 0.0052). Next, global transcriptome sequencing (RNA-seq) experiments were conducted to explore gene expression and alternative splicing profiles regulated by hnRNP K. It is shown that upregulated genes by hnRNP K-OE were associated with inflammatory response and an apoptotic process of neuron cells, which involves in cancer. In addition, the alternative splicing of those genes regulated by hnRNP K-OE was associated with transcriptional regulation. Analysis of the binding features of dysregulated transcription factors (TFs) in the promoter region of the inflammatory response genes regulated by hnRNP K revealed that hnRNP K may modulate the expression level of genes related to inflammatory response by influencing the alternative splicing of TFs. Among these hnRNP K-TFs-inflammatory gene regulatory networks, quantitative reverse transcription polymerase chain reaction (RT-qPCR) experiments and gene silencing were conducted to verify the hnRNP K-IRF1-CCL5 axis. In conclusion, the hnRNP K-TFs-inflammatory gene regulatory axis provides a novel molecular mechanism for hnRNP K in promoting CESC and offers a new therapeutic target.
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http://dx.doi.org/10.1177/15353702221110649DOI Listing
July 2022

Unusual mature teratomas in the right ventricle.

Eur Heart J Cardiovasc Imaging 2022 Jul 19. Epub 2022 Jul 19.

Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University; Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), No. 2, Anzhenli, Chaoyang District, Beijing 100029, People's Republic of China.

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http://dx.doi.org/10.1093/ehjci/jeac144DOI Listing
July 2022
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