Publications by authors named "Chelsea den Hollander"

2 Publications

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Expression of Plet1 controls interstitial migration of murine small intestinal dendritic cells.

Eur J Immunol 2019 02 14;49(2):290-301. Epub 2018 Dec 14.

Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.

Under homeostatic conditions, dendritic cells (DCs) continuously patrol the intestinal lamina propria. Upon antigen encounter, DCs initiate C-C motif chemokine receptor 7 (CCR7) expression and migrate into lymph nodes to direct T cell activation and differentiation. The mechanistic underpinnings of DC migration from the tissues to lymph nodes have been largely elucidated, contributing greatly to our understanding of DC functionality and intestinal immunity. In contrast, the molecular mechanisms allowing DCs to efficiently migrate through the complex extracellular matrix of the intestinal lamina propria prior to antigen encounter are still incompletely understood. Here we show that small intestinal murine CD11b CD103 DCs express Placenta-expressed transcript 1 (Plet1), a glycophoshatidylinositol (GPI)-anchored surface protein involved in migration of keratinocytes during wound healing. In the absence of Plet1, CD11b CD103 DCs display aberrant migratory behavior, and accumulate in the small intestine, independent of CCR7 responsiveness. RNA-sequencing indicated involvement of Plet1 in extracellular matrix-interactiveness, and subsequent in-vitro migration assays revealed that Plet1 augments the ability of DCs to migrate through extracellular matrix containing environments. In conclusion, our findings reveal that expression of Plet1 facilitates homeostatic interstitial migration of small intestinal DCs.
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February 2019

Effective Single Photodynamic Treatment of Onychomycosis Using a Multifunctional Porphyrin Photosensitizer and Green Light.

J Fungi (Basel) 2015 Jul 27;1(2):138-153. Epub 2015 Jul 27.

Department of Radiotherapy, Erasmus Medical Centre, P.O. Box 2040, Office Ee-1683, 3000-CA Rotterdam, The Netherlands.

Onychomycosis is predominantly caused by the dermatophytes , and The main treatment obstacle concerns low nail-plate drug permeability. antifungal photodynamic treatment (PDT) and nail penetration enhancing effectiveness have been proven for multifunctional photosensitizer 5,10,15-(4--methylpyridinium)-20-(4-(butyramido-methylcysteinyl)-hydroxyphenyl)-[21,23]-porphine trichloride (PORTHE). This study investigates single PORTHE green laser/LED PDT of varying degrees of onychomycoses in a human nail model. , , onychomycoses were induced on nail pieces at 28 °C (normal air) and 37 °C (6.4% CO₂) during 3 to 35 days and PDTs applied to the 37 °C infections. All dermatophytes showed increasingly nail plate invasion at 37 °C between 7 and 35 days; arthroconidia were observed after 35 days for and . Using 81 J/cm² (532 nm) 7-day onychomycoses were cured (92%) with 80 µM PORTHE (pH 8) after 24 h propylene glycol (PG, 40%) pre-treatment and 35-day onychomycoses (52%-67%) with 24 h PORTHE (40-80 µM)/40% PG treatment (pH 5). 28 J/cm² LED light (525 ± 37 nm) improved cure rates to 72%, 83% and 73% for, respectively, , and 35-day onychomycoses and to 100% after double PDT. Data indicate PDT relevance for onychomycosis.
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July 2015