Publications by authors named "Chatuphon Siamphan"

6 Publications

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Towards a Miniaturized Culture Screening for Cellulolytic Fungi and Their Agricultural Lignocellulosic Degradation.

J Microbiol Biotechnol 2020 Nov;30(11):1670-1679

National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Klong Luang, Pathumthani 12120, Thailand.

The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: (3 strains: AG466, AG438 and AG499), (4 strains: AG452, AG496, AG498 and AG559), (1 strain: AG548) and (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.
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http://dx.doi.org/10.4014/jmb.2007.07005DOI Listing
November 2020

Oceanobacillus arenosus sp. nov., a moderately halophilic bacterium isolated from marine sand.

Int J Syst Evol Microbiol 2015 Sep 28;65(9):2943-2948. Epub 2015 May 28.

Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterium, designated CAU 1183(T), was isolated from marine sand and its taxonomic position was investigated by using a polyphasic approach. The bacterium grew optimally at 30 °C, at pH 8.5 and in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1183(T) formed a distinct lineage within the genus Oceanobacillus and exhibited the highest similarity to Oceanobacillus chungangensis CAU 1051(T) (97.6%). The strain contained MK-7 as the predominant isoprenoid quinone and anteiso-C15 : 0 was the major cellular fatty acid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipid pattern of strain CAU 1183(T) consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified lipids, including two phospholipids, two glycolipids, a phosphoglycolipid and two lipids. The G+C content of the genomic DNA was 37.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CAU 1183(T) should be assigned to a novel species in the genus Oceanobacillus, for which the name Oceanobacillus arenosus sp. nov. is proposed. The type strain is CAU 1183(T) ( = KCTC 33037(T) = CECT 8560(T)).
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http://dx.doi.org/10.1099/ijs.0.000360DOI Listing
September 2015

Eudoraea chungangensis sp. nov., isolated from an aquafarm waste water sludge.

Antonie Van Leeuwenhoek 2015 Apr 28;107(4):1009-15. Epub 2015 Jan 28.

Department of Microbiology, College of Medicine, Chung-Ang University (CAU), Seoul, South Korea.

A Gram-stain negative, non-spore-forming, non-motile, strictly aerobic bacterial strain, designated CAU 1296(T), was isolated from an aquafarm waste water sludge and its taxonomic position was investigated using a polyphasic approach. Cells of strain CAU 1296(T) grew optimally at 30 °C, at pH 7.5 and in the presence of 4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1296(T) formed a distinct lineage within the genus Eudoraea and exhibited similarity to Eudoraea adriatica AS06/20a(T) (95.4 % similarity). The major cellular fatty acids of the isolate were iso-C15:0, iso-C15:1 G, and summed feature 3 (C16:1 ω6c and C16:1 ω7c). The polar lipid pattern of strain CAU 1296(T) consisted of phosphatidylethanolamine, and unidentified lipids including a phosphoglycolipid, phospholipid, glycolipid, aminophospholipid, two aminolipids, and six further unidentified lipids. The strain contained menaquinone-6 (MK-6) as the major isoprenoid quinone. The G+C content of the genomic DNA was 38.7 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain CAU 1296(T) should be classified as a novel species in the genus Eudoraea, for which the name Eudoraea chungangensis sp. nov. is proposed. The type strain is CAU 1296(T) (=KCTC 42048(T), =CCUG 66239(T), =CECT 8744(T)).
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http://dx.doi.org/10.1007/s10482-015-0393-7DOI Listing
April 2015

Salegentibacter chungangensis sp. nov., isolated from a sea sand and emended description of the genus Salegentibacter.

Int J Syst Evol Microbiol 2014 May 29;64(Pt 5):1514-1519. Epub 2014 Jan 29.

Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

A Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterial strain, designated CAU 1289T, was isolated from a marine sand and its taxonomic position was investigated using a polyphasic approach. It grew optimally at pH 6.5 and 30 °C and in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1289T belonged to the genus Salegentibacter, exhibiting sequence similarity values of 93.6-96.3% to members of this genus, and was related most closely to Salegentibacter mishustinae KMM 6049T (96.3% similarity). Strain CAU 1289T contained MK-6 as the predominant menaquinone. The major fatty acid was iso-C15:0. The cell-wall peptidoglycan of strain CAU 1289T contained meso-diaminopimelic acid. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid, an unidentified aminophospholipid, an unidentified glycolipid and ten unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1289T is considered to represent a novel species of the genus Salegentibacter, for which the name Salegentibacter chungangensis sp. nov. is proposed. The type strain is CAU 1289T (KCTC 24000T=CCUG 64793T). An emended description of the genus Salegentibacter is also proposed.
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http://dx.doi.org/10.1099/ijs.0.059170-0DOI Listing
May 2014

Sneathiella chungangensis sp. nov., isolated from a marine sand, and emended description of the genus Sneathiella.

Int J Syst Evol Microbiol 2014 May 21;64(Pt 5):1468-1472. Epub 2014 Jan 21.

Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

A Gram-stain-negative, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1294T, was isolated from a sand sample and its taxonomic position was investigated using a polyphasic approach. The strain grew optimally at pH 6.5 and 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1294T formed a lineage with member of the genus Sneathiella and exhibited similarity to Sneathiella glossodoripedis MKT133T (96.3% similarity) and Sneathiella chinensis LMG 23452T (95.1% similarity). Strain CAU 1294T contained Q-10 as the predominant respiratory quinone. C18:1ω7c, C16:0 and cyclo-C19:0ω8c were the major cellular fatty acids. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine and two unidentified phospholipids. The DNA G+C content was 56.6 mol%. On the basis of these results, strain CAU 1294T is considered to represent a novel species of the genus Sneathiella, for which the name Sneathiella chungangensis is proposed. The type strain is CAU 1294T (=KCTC 32476T=CECT 8513T). An emended description of the genus Sneathiella is also proposed.
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http://dx.doi.org/10.1099/ijs.0.059386-0DOI Listing
May 2014