Publications by authors named "Chaohong Huang"

11 Publications

  • Page 1 of 1

TRAIL-Armed ER Nanosomes Induce Drastically Enhanced Apoptosis in Resistant Tumor in Combination with the Antagonist of IAPs (AZD5582).

Adv Healthc Mater 2021 Jun 8;10(11):e2100030. Epub 2021 May 8.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 51006, China.

Although mesenchymal stem cells (MSCs) can be engineered to deliver the TNF-related apoptosis-inducing ligand (TRAIL) as an effective anticancer therapy, the clinical application is hampered by the costly manufacturing of therapeutic MSCs. Therefore, it is needed to find an alternative cell-free therapy. In this study, TRAIL-armed endoplasmic reticulum (ER)-derived nanosomes (ERN-T) are successfully prepared with an average size of 70.6 nm in diameter from TRAIL transduced MSCs. It is demonstrated that the ERN-T is significantly more efficient for cancer cell killing than the soluble recombinant TRAIL (rTRAIL). AZD5582 is an antagonist of the inhibitors of apoptosis proteins (IAPs), and its combination with ERN-T induces strikingly enhanced apoptosis in cancerous but not normal cells. AZD5582 sensitizes resistant cancer cells to TRAIL through concomitant downregulation of IAP members like XIAP and the Bcl2 family member Mcl-1. Intravenously infused ERN-Ts accumulate in tumors for over 48 h indicating good tumor tropism and retention. The combination of ERN-T and AZD5582 drastically promotes therapeutic efficacy comparing with the cotreatment by rTRAIL and AZD5582 in a subcutaneous MDA-MB-231 xenograft tumor model. The data thus demonstrate that ERN-T can be a novel cell-free alternative to TRAIL-expressing MSC-based anticancer therapy and its efficacy can be drastically enhanced through combination with AZD5582.
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http://dx.doi.org/10.1002/adhm.202100030DOI Listing
June 2021

Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib.

Cancers (Basel) 2020 May 4;12(5). Epub 2020 May 4.

Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 51006, China.

Tumour necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) is a promising anti-cancer agent that rapidly induces apoptosis in cancer cells. Unfortunately, the clinical application of recombinant TRAIL (rTRAIL) has been hampered by its common cancer resistance. Naturally TRAIL is delivered as a membrane-bound form by extracellular vesicles (EV-T) and is highly efficient for apoptosis induction. SCH727965 (dinaciclib), a potent cyclin-dependent kinase (CDK) inhibitor, was shown to synergize with other drugs to get better efficacy. However, it has never been investigated if dinaciclib coordinates with EV-T to enhance therapeutic results. This study explores the potential of combination therapy with EV-T and dinaciclib for cancer treatment. EV-T was successfully derived from human TRAIL transduced cells and shown to partially overcome resistance of A549 cells. Dinaciclib was shown to drastically enhance EV-T killing effects on cancer lines that express good levels of death receptor (DR) 5, which are associated with suppression of CDK1, CDK9 and anti-apoptotic proteins. Combination therapy with low doses of EV-T and dinaciclib induced strikingly enhanced apoptosis and led to complete regression in A549 tumors without any adverse side effects observed in a subcutaneous xenograft model. Tumor infiltration of mass NK cells and macrophages was also observed. These observations thus indicate that the combination of EV-T with dinaciclib is a potential novel therapy for highly effective and safe cancer treatment.
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http://dx.doi.org/10.3390/cancers12051157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281120PMC
May 2020

Prolactin and Thyroid Stimulating Hormone (TSH) Levels and Sexual Dysfunction in Patients with Schizophrenia Treated with Conventional Antipsychotic Medication: A Cross-Sectional Study.

Med Sci Monit 2018 Dec 16;24:9136-9143. Epub 2018 Dec 16.

Sixth Affiliated Hospital, Kunming Medical University, Yuxi, Yunnan, China (mainland).

BACKGROUND This study aimed to investigate the relationship between serum profiles of prolactin and thyroid stimulating hormone (TSH) and sexual dysfunction in patients with schizophrenia treated with conventional antipsychotic medication. MATERIAL AND METHODS A hospital-based cross-sectional study included 118 patients, age range 18-57 years (55 men, 63 women), with a confirmed diagnosis of schizophrenia. All patients were stable after antipsychotic treatment. Serum levels of hormones, including prolactin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone, testosterone, thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), free triiodothyronine (FT3) and free thyroxine (FT4), were detected in venous blood. The Positive and Negative Syndrome Scale (PANSS) score was used to measure symptom severity of patients with schizophrenia. The Mandarin Chinese version of the Arizona Sexual Experience Scale (ASEX), a 5-item scale, was used to measure sexual function. RESULTS There were 66 patients (55.9%) who had hyperprolactinemia, the prevalence of hyperprolactinemia was markedly higher in the sexual dysfunction group than the non-sexual dysfunction group (91.8% vs. 17.5%) (P<0.001). Mean prolactin levels were significantly increased in patients with sexual dysfunction compared with the patients without sexual dysfunction (P<0.001), with a higher incidence in female patients. Subclinical hypothyroidism and hyperprolactinemia were found to be independently associated with sexual dysfunction, and an increased PANSS negative score was an independent risk factor for the development of sexual dysfunction. CONCLUSIONS The incidence of sexual dysfunction was significantly increased in patients with schizophrenia. Hyperprolactinemia and subclinical hypothyroidism were associated with sexual dysfunction, especially in female patients.
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http://dx.doi.org/10.12659/MSM.913759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319142PMC
December 2018

Hyperbolic accelerating beams and their relation with Hermite-Gaussian beams.

J Opt Soc Am A Opt Image Sci Vis 2018 Feb;35(2):262-266

We derive the initial distributions of phase and complex amplitude of accelerating beams with arbitrary predesigned hyperbolic trajectories using the caustic-design method and explore the relation between these beams and Hermite-Gaussian beams. The results show the hyperbolic accelerating beams are a larger class of beams than Hermite-Gaussian beams. When the bending parameter is an integer, the hyperbolic accelerating beams have a similar initial complex amplitude distribution and almost the same propagating characteristics as Hermite-Gaussian beams. Through the analysis of the ray-based method, we also derive an approximate expression for the initial complex amplitude of Hermite-Gaussian beams after introducing an amplitude distribution function. Although the proposed approximate expressions of complex amplitude are more complex than the usually used Hermite-Gaussian function, they explicitly indicate the information on local amplitude, wave vector, and internal ray structure (including caustics) of these beams and thus provide us clearer geometrical insights into these beams.
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http://dx.doi.org/10.1364/JOSAA.35.000262DOI Listing
February 2018

Orbital angular momentum and paraxial propagation characteristics of non-coaxial Laguerre-Gaussian beams.

J Opt Soc Am A Opt Image Sci Vis 2016 Nov;33(11):2137-2143

The orbital angular momentum (OAM) and paraxial propagation characteristics of non-coaxial Laguerre-Gaussian (LG) beams are discussed. In the initial plane, the amplitudes of non-coaxial LG modes are the product of a Laguerre polynomial, a vortex phase factor, and a non-coaxial Gaussian window function. Because of displacement between the centroid and vortex center of the beams, the non-coaxial LG beams demonstrate a non-zero extrinsic OAM, which causes the propagation of beam centroid to have a small tilt angle relative to the z axis. Through separating the extrinsic OAM from the total OAM carried by the non-coaxial LG beams, the tilt angle and trajectory of the beam centroid are derived. Furthermore, the paraxial propagating characteristics of these beams are also discussed analytically. The results show the vortex center initially at origin demonstrates a linear lateral shift with propagation and the peak of transverse intensity has a trajectory of a straight line, although the intensity profile rotates itself with propagation.
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http://dx.doi.org/10.1364/JOSAA.33.002137DOI Listing
November 2016

Noncoaxial Bessel-Gauss beams.

J Opt Soc Am A Opt Image Sci Vis 2016 04;33(4):508-12

We proposed a new family of noncoaxial Gauss-truncated Bessel beams through multiplying conventional symmetrical Bessel beams by a noncoaxial Gauss function. These beams can also be regarded as the exponential-truncated version of Bessel-Gauss beams since they can be transformed into the product of Bessel-Gauss beams and an exponential window function along a certain Cartesian axis. The closed-form solutions of the angular spectra and paraxial propagation of these beams were derived. These beams have asymmetrical intensity distributions and carry the same orbit angular momentum per photon as the corresponding Bessel-Gauss beams. While propagating along the z axis, the mth (m≠0) noncoaxial Bessel-Gauss beams rotate their intensity distributions and the mth-order vortex at the beam center has a transverse shift along the direction perpendicular to the offset axis. Depending on the product of the transverse scalar factor of the Bessel beams and the offset between the Gaussian window function and the center of the Bessel beams, the noncoaxial Bessel-Gauss beams can produce unit vortices with opposite signs in pairs during propagation.
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http://dx.doi.org/10.1364/JOSAA.33.000508DOI Listing
April 2016

Accelerating propagation properties of misplaced Hermite-Gaussian beams.

J Opt Soc Am A Opt Image Sci Vis 2014 Aug;31(8):1762-5

A new family of finite-energy accelerating beams was constructed through misplacing the Hermite polynomial and Gaussian window function. The closed-form solution of k-space spectra and paraxial propagation of these beams are derived from the Fourier transform and the scalar angle spectra integral. These beams have similar propagation properties to finite Airy beams and parabolic beams, but the accelerating trajectory is hyperbola rather than parabola. The beam family can be experimentally generated by exponentially truncating the high-order Hermite-Gaussian beams in the spatial domain.
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http://dx.doi.org/10.1364/JOSAA.31.001762DOI Listing
August 2014

Fast and noninterpolating method for subpixel displacement analysis of digital speckle images using phase shifts of spatial frequency spectra.

Appl Opt 2014 May;53(13):2806-14

A fast noninterpolation method for calculating displacement of digital speckle images with subpixel precision was introduced. In this method, the precise displacement is obtained from phase shifts of spatial frequency spectra of two digital speckle images instead of digital correlation calculation. First, digital speckle images before and after displacement are windowed and fast Fourier transform is performed. Then, phase shifts of different spatial frequencies are linearly fitted in spectral space using the least square method, and a coarse displacement value is directly calculated according to the phase shift theorem of Fourier transform. By a window technique and iterative procedure, the influence of finite image size on the accuracy of the results is eliminated, and the accurate displacement is obtained finally. It is significant that the method obtains the subpixel-precision displacement without any interpolation operations. The test results show that the method has high computing efficiency, high precision, and good robustness to low image quality.
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http://dx.doi.org/10.1364/AO.53.002806DOI Listing
May 2014

Upregulation of blood proBDNF and its receptors in major depression.

J Affect Disord 2013 Sep 26;150(3):776-84. Epub 2013 Mar 26.

Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan Province, PR China.

Background: In recent decades, the role of brain-derived neurotrophic factor (BDNF) in depression has received intensive attention. However, the relationship between proBDNF and depression has not been clearly elucidated.

Methods: Forty drug-free women patients diagnosed with major depression and 50 healthy female controls were enrolled in our study. Peripheral blood was sampled from all the subjects. With the blood samples, we assessed the relationship between BDNF and major depression from following aspects: the levels of BDNF, proBDNF and their receptors in the sera and lymphocytes. The mRNA levels of these factors in lymphocytes were also examined. Furthermore, the correlations between each factor and the severity of major depression were tested.

Results: It was found that: (a) the protein and serum levels of proBDNF, sortilin and p75NTR were higher in major depressive patients than in healthy controls while mature BDNF and TrkB levels were lower; (b) the BDNF, TrkB, sortilin and p75NTR mRNA levels changed in line with their protein levels; (c) The levels of mature BDNF and TrkB had negative correlations with the major depression severity, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the scores of HRSD-21; (d) the ratio of proBDNF and mBDNF was imbalanced in major depressive patients.

Conclusion: The balance between the proBDNF/p75NTR/sortilin and mBDNF/TrkB signaling pathways appears dysregulated in major depression and both pathways should be considered as biomarkers for the major depression

Limitations: More cases on both genders should be enrolled in our study. And further works on the mechanisms of how BDNF and its receptors are regulated in depression should also be carried out.
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http://dx.doi.org/10.1016/j.jad.2013.03.002DOI Listing
September 2013

Stable and spacing-adjustable multiwavelength Raman fiber laser based on mixed-cascaded phosphosilicate fiber Raman linear cavity.

Opt Lett 2008 Jul;33(14):1602-4

Department of Electronic Engineering, Institute of Optoelectronic Technology, Xiamen University, Xiamen 361005, China.

A novel multiwavelength Raman fiber laser based on the mixed-cascaded Stokes effects of phosphosilicate fiber is proposed and demonstrated experimentally. By using stimulated Raman scattering of both P(2)O(5) and SiO(2) along 1 km phosphosilicate fiber pumped with a 1064 nm double-clad fiber laser, the mixed-cascaded Raman linear cavity is formed by a pair of fiber Bragg gratings at 1239 nm, a polarization-maintaining fiber (PMF) Sagnac loop filter, and a conventional optical loop mirror. Up to 15-wavelength stable oscillations around 1320 nm are obtained with a wavelength spacing of 0.44 nm and power nonuniformity of less than 4 dB. By changing the length of the PMF in the Sagnac loop filter from 10 to 5.5 m, the wavelength spacing is adjustable from 0.44 to 0.8 nm. The extinction ratio of the laser is more than 30 dB. Excellent stability is also observed with a peak power fluctuation of less than 0.8 dB in 1 h.
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http://dx.doi.org/10.1364/ol.33.001602DOI Listing
July 2008

Explicit solution for Raman fiber laser using Lambert W function.

Opt Express 2007 Apr;15(8):4671-6

Department of Electronic Engineering, Xiamen University, Xiamen 361005, China.

In this paper, an approximate explicit solution for the first-order Raman fiber laser is obtained by using Lambert W function. Good agreement between the explicit solution and numerical simulation is demonstrated. Furthermore, the optimal design of Raman fiber laser is discussed using the proposed solution. The optimal values of fiber length, reflectivity of output fiber Bragg grating and power transfer efficiency are obtained under different pump power. There exists a certain tolerance of the optimal parameters, in which the output power decreases only slightly. The optimal fiber length and reflectivity of output FBG decrease with increasing pump power.
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http://dx.doi.org/10.1364/oe.15.004671DOI Listing
April 2007