Publications by authors named "Chao-Ying Wang"

24 Publications

  • Page 1 of 1

Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model.

Sci Rep 2021 May 11;11(1):9924. Epub 2021 May 11.

Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, No.161, Sec. 6, Minquan E. Rd., Neihu Dist., Taipei, 11490, Taiwan.

The purpose of present study was to longitudinally investigate the alterations in infrapatellar fat pad (IPFP) vascularity in 5/6 nephrectomized rats by using dynamic contrast enhanced (DCE) MRI and IPFP degeneration by using MRI T2* relaxation time. Twelve male Sprague-Dawley rats were assigned to a control group and a 5/6 nephrectomy CKD group. The right knees of all rats were longitudinally scanned by 4.7 T MRI, and serial changes in the IPFP were assessed at 0, 8, 16, 30, and 44 weeks by DCE-MRI (parameters A, k and k) and MRI T2* mapping. After MRI measurements, knee specimens were obtained and evaluated histologically. The CKD group had IPFPs with lower blood volume A and lower permeability k values from 16 weeks (p < 0.05), lower venous washout k value from 30 weeks (p < 0.001), and significantly higher T2* values reflecting adipocyte degeneration beginning at 16 weeks (p < 0.05). The histopathological results confirmed the MRI findings. Hypoperfusion and adipocytes degeneration related to CKD were demonstrated in a rodent 5/6 nephrectomy model. DCE parameters and MRI T2* can serve as imaging biomarkers of fat pad degeneration during CKD progression.
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http://dx.doi.org/10.1038/s41598-021-89336-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113578PMC
May 2021

The complete chloroplast genome of (Lamiales, Lamiaceae), an ornamental and medicinal plant from Chongqing, China.

Mitochondrial DNA B Resour 2021 Mar 24;6(3):1229-1230. Epub 2021 Mar 24.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China.

Plants classified to the genus L. have important medicinal and ornamental value. Here, we report and characterize the complete chloroplast (cp) genome of to provide molecular basis for the further studies on the phylogeny analysis of this genus. The cp genome is 154,183 bp in length and is organized with a typical quadripartite structure, containing two inverted repeats of 25,701 bp separated by a large single-copy region of 84,956 bp and a small single-copy region of 17,825 bp. The cp genome of contains 112 distinct genes, including 78 protein-coding, 30 tRNAs and 4 rRNAs genes. The phylogenetic analysis showed that is fully resolved in a clade with , sister to the clade of and var. .
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http://dx.doi.org/10.1080/23802359.2021.1875932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995876PMC
March 2021

The complete chloroplast genome of (Ericaceae): an endemic and endangered species from China.

Mitochondrial DNA B Resour 2021 Jan 19;6(1):196-197. Epub 2021 Jan 19.

Key Laboratory of Horticulture Science for Southern Mountainous Regions, College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.

Diels (Ericaceae) is a Chinese endemic and endangered species with high ornamental value. Here the complete chloroplast (cp) genome of was assembled and characterized. The cp genome is in a total length of 201,047 bp with the typical quadripartite structure of angiosperms, containing two inverted repeats (IRs) of 44,650 bp separated by a large single-copy (LSC) region of 109,134 bp and a small single-copy (SSC) region of 2613 bp. The whole cp genome of contains 143 genes, including 93 protein-coding genes, 42 transfer RNA genes and 8 ribosomal RNA genes. Phylogenetic analysis based on the coding sequences of cp genome within the Ericaceae family suggests that is closely related to .
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http://dx.doi.org/10.1080/23802359.2020.1860714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832514PMC
January 2021

Admission Neutrophil-Lymphocyte Ratio Predicts Rebleeding Following Aneurismal Subarachnoid Hemorrhage.

World Neurosurg 2020 06 27;138:e317-e322. Epub 2020 Feb 27.

Department of Neurosurgery, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, China; Department of Neurosurgery, Dehua County Hospital, Quanzhou, China; Department of Neurosurgery, Dehua County Hospital of HuaQiao University, Quanzhou, China. Electronic address:

Objective: The relationship between neutrophil-lymphocyte ratio (NLR) and the occurrence of rebleeding in aneurysmal subarachnoid hemorrhage (aSAH) is poorly understood. Our study aimed to investigate the association between NLR on admission and rebleeding following aSAH.

Methods: Clinical and laboratorial data from patients with aSAH were retrospectively collected, including leukocyte, neutrophil, lymphocyte, and NLR. Univariate and multivariate analyses were performed to assess for the association of NLR with rebleeding. We performed propensity-score matching analyses to correct imbalances in patient characteristics between the rebleeding group and nonrebleeding group.

Results: Rebleeding occurred in 30 of 716 (4.19%) patients with aSAH in this cohort. Patients with rebleeding had significantly higher NLR comparing with patients without rebleeding (11.27 vs. 5.5; P < 0.05) in the univariate analysis. In the multivariate analysis, NLR was considered as a risk factor of rebleeding (odds ratio, 0.283; 95% confidence interval, 0.130-0.620; P = 0.002), as well as Fisher grade (odds ratio, 0.353, 95% confidence interval, 0.151-0.824; P = 0.016). The area under the curve of the NLR and combined NLR-Fisher grade model was 0.702 and 0.744 (sensitivity was 39.94%, and specificity was 100%) for predicting rebleeding, respectively. After propensity-score matching, the optimal cutoff value for NLR as a predictor for rebleeding following aSAH was determined as 5.4 (sensitivity was 83.33%, and the specificity was 63.33%).

Conclusions: Higher NLR predicts the occurrence of rebleeding and poor outcome, and NLR combined with Fisher grade significantly improves the prediction of rebleeding following aSAH.
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http://dx.doi.org/10.1016/j.wneu.2020.02.112DOI Listing
June 2020

Histogram analysis of T2*-based pharmacokinetic imaging in cerebral glioma grading.

Comput Methods Programs Biomed 2018 Mar 15;155:19-27. Epub 2017 Nov 15.

Research Center of Translational Imaging, College of Medicine, Taipei Medical University, Taipei, Taiwan; Radiogenomic Research Center, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan; Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Department of Medical Imaging, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan; Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan. Electronic address:

Background And Objective: To investigate the feasibility of histogram analysis of the T2*-based permeability parameter volume transfer constant (K) for glioma grading and to explore the diagnostic performance of the histogram analysis of K and blood plasma volume (v).

Methods: We recruited 31 and 11 patients with high- and low-grade gliomas, respectively. The histogram parameters of K and v, derived from the first-pass pharmacokinetic modeling based on the T2* dynamic susceptibility-weighted contrast-enhanced perfusion-weighted magnetic resonance imaging (T2* DSC-PW-MRI) from the entire tumor volume, were evaluated for differentiating glioma grades.

Results: Histogram parameters of K and v showed significant differences between high- and low-grade gliomas and exhibited significant correlations with tumor grades. The mean K derived from the T2* DSC-PW-MRI had the highest sensitivity and specificity for differentiating high-grade gliomas from low-grade gliomas compared with other histogram parameters of K and v.

Conclusions: Histogram analysis of T2*-based pharmacokinetic imaging is useful for cerebral glioma grading. The histogram parameters of the entire tumor K measurement can provide increased accuracy with additional information regarding microvascular permeability changes for identifying high-grade brain tumors.
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http://dx.doi.org/10.1016/j.cmpb.2017.11.011DOI Listing
March 2018

Knee subchondral bone perfusion and its relationship to marrow fat and trabeculation on multi-parametric MRI and micro-CT in experimental CKD.

Sci Rep 2017 06 8;7(1):3073. Epub 2017 Jun 8.

Department of Radiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.

The pathogenesis of chronic kidney disease (CKD) is multifactorial. In the progression of CKD arthropathy, arteriosclerosis may alter the knee subchondral bone marrow by altering blood flow through the bone vasculature. Herein, multi-parametric MRI assessment, including dynamic contrast enhanced magnetic resonance imaging (DCE-MRI), magnetic resonance spectroscopy (MRS), MRI T2*, contrast enhanced MR angiography (CE-MRA), and micro-CT were applied in a rodent nephrectomy model to: 1) investigate the blood perfusion of subchondral bone marrow and its relationship to fat water content and trabeculation pattern in CKD and 2) demonstrate the feasibility of using multi-parametric MRI parameters as imaging biomarkers to evaluate the disease's progression. Two groups of rats in our study underwent either 1) no intervention or 2) 5/6 nephrectomy. We found that in the CKD group, perfusion amplitude A and elimination constant k values were significantly decreased, and vascular permeability k was significantly increased. MRS showed that fat fraction (FF) was significantly lower, water fraction (WF) was significantly higher in the CKD group. Micro-CT showed a significant loss of trabecular bone. Knee subchondral bone marrow perfusion deficiency in experimental CKD may be associated with decreased fat content, increased water content, and sparse trabeculation.
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http://dx.doi.org/10.1038/s41598-017-03059-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465086PMC
June 2017

Construction of recombinant Yarrowia lipolytica and its application in bio-transformation of lignocellulose.

Bioengineered 2017 Sep 17;8(5):624-629. Epub 2017 Feb 17.

a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.

Lignocellulose is a polysaccharide and an abundant biomass resource that widely exists in grains, beans, rice, and their by-products. Over 10 million tons of lignocellulose resources and processing products are produced every year in China. Three recombinant Y. lipolytica strains with cellulase (β-glucosidase, endoglucanase and cellobiohydrolase) were constructed. The enzymatic activities of these enzymes were 14.181 U/mL, 16.307 U/mL, and 17.391 U/mL, respectively. The whole cell cellulases were used for a stover bio-transformation. The celluloses in the stover were partly degraded by the cellulases, and the degradation products were transformed into single cell protein (SCP) by the Y. lipolytica cells. After 15 d of fermentation with the whole cell cellulases, the protein content of the maize stover and the rice straw reached 16.23% and 14.75%, which increased by 168.26% and 161.52% compared with the control, respectively. This study provides a new stage for the efficient utilization of stover in the feed industry.
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http://dx.doi.org/10.1080/21655979.2017.1293219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639843PMC
September 2017

Interleukin 26 suppresses receptor activator of nuclear factor κB ligand induced osteoclastogenesis via down-regulation of nuclear factor of activated T-cells, cytoplasmic 1 and nuclear factor κB activity.

Rheumatology (Oxford) 2016 Nov 21;55(11):2074-2083. Epub 2016 Aug 21.

Department and Graduate institute of Biology and Anatomy

Objective: IL-26 has been shown to have high expression in RA. However, the effects of IL-26 on bone destruction in RA have not been evaluated. The aim of this study was to investigate the effects and mechanisms of IL-26 on RANK ligand (RANKL)-induced osteoclastogenesis.

Methods: We treated cells with IL-26 in RANKL-induced oseteoclastogenesis to monitor osteoclast formation by tartrate-resistant acid phosphatase (TRAP) staining. Osteoclast activity was assessed by pit formation assay and F-actin ring formation. The mechanism of the inhibition was studied by biochemical analyses such as RT-PCR, immunofluorescence staining and immunoblotting. In addition, cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Results: IL-26 inhibited RANKL-induced TRAP-positive multinucleated cells and inhibited RANKL-induced nuclear factor κB (NF-κB) activation and nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) nuclear translocation in RAW264.7 cells. Also, IL-26 significantly inhibited the bone-resorbing activity and F-actin ring formation ability of mature osteoclasts. Moreover, IL-26 suppressed RANKL-induced mitogen-activated protein kinase activation and NFATc1 downstream gene expression.

Conclusion: We suggest that the inhibitory activity of IL-26 on osteoclastogenesis is via down-regulation of RANKL-induced NF-κB and NFATc1 expression. Our results suggest IL-26 as a possible new remedy against osteolytic bone destruction.
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http://dx.doi.org/10.1093/rheumatology/kew302DOI Listing
November 2016

Change in T2* relaxation time of Hoffa fat pad correlates with histologic change in a rat anterior cruciate ligament transection model.

J Orthop Res 2015 Sep 18;33(9):1348-55. Epub 2015 May 18.

Department of Radiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.

The Hoffa fat pad (infrapatellar fat pad) is a source of post-traumatic anterior knee pain, and Hoffa disease is a syndrome leading to chronic inflammation of the fat pad. Herein, change in T2* relaxation time of the fat pad was measured in a rodent anterior cruciate ligament transection (ACLX) model in order to (i) examine the causal relationship of anterior cruciate ligament (ACL) deficiency and Hoffa disease and (ii) demonstrate the feasibility of using T2* as an imaging biomarker to monitor disease progression. Three groups of male Sprague Dawley rats (n = 6 each group), received either (i) no intervention; (ii) sham surgery at the right knee; or (iii) right ACLX. T2* relaxation time was measured and histology was examined in the Hoffa fat pad after surgery. At 13 and 18 weeks after surgery, T2* values were significantly higher in the right fat pad than the left (p < 0.001) and significantly higher in the ACLX group than the control and sham groups (p < 0.001). Histology showed fibrosis and degeneration of adipocytes in the right knees of the ACLX group. We conclude that ACL deficiency and Hoffa disease are causally related and that MRI T2* value can serve as an imaging biomarker of Hoffa disease progression.
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http://dx.doi.org/10.1002/jor.22914DOI Listing
September 2015

[Effects of flooding on the photosynthetic physiology characteristics of Pterocarya stenoptera seedlings].

Ying Yong Sheng Tai Xue Bao 2013 Mar;24(3):675-82

Ministry of Education Key Laboratory for the Eco-Environment of the Three Gorges Reservoir Region, Chongqing 400715, China.

Three water treatments (control, CK; continuous flooding, CF; and periodic flooding, PF) were installed to simulate the soil moisture change of the riparian zone in the Three Gorges Reservoir (TGR) region of China to study the physiological and ecological adaptation mechanisms of the native plant species Pterocarya stenoptera seedlings to the soil moisture change. All the water treatments had significant effects on the photosynthesis, biomass accumulation, and growth of the P. stenoptera seedlings. As compared with those in CK, the seedling's net photosynthetic rate (Pn) and stomatal conductance (gs) in treatments CF and PF were significantly lower while the intercellular carbon dioxide concentration was significantly higher, and the Pn and gs in CF and PF declined initially followed by a gradual recovery or tended to be stable. With the extension of treatment time, the total biomass and root-, stem-, and leaf biomass, plant height, and basal diameter in CF and PF all presented an increasing trend. Throughout the experimental period, the total biomass, root- and leaf biomass, and plant height in CF and PF and the stem biomass in PF were significantly lower than those in CK, whereas the stem biomass in CF had no significant difference with that in CK, though the basal diameter in CF was significantly higher than that in CK. It was suggested that the P. stenoptera seedlings had the physiological and ecological characteristics of tolerating flooding rather than flooding-drought alteration.
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March 2013

Effects of microvascular permeability changes on contrast-enhanced T1 and pharmacokinetic MR imagings after ischemia.

Stroke 2013 Jul 6;44(7):1872-7. Epub 2013 Jun 6.

Department of Radiology, Tri-Service General Hospital, Taipei, Taiwan.

Background And Purpose: Brain enhancement on contrast-enhanced T1-weighted imaging (CET1-WI) after ischemic stroke is generally accepted as an indicator of the blood-brain barrier disruption. However, this phenomenon usually starts to become visible at the subacute phase. The purpose of this study was to evaluate the time-course profiles of K(trans), cerebral blood volume (vp), and CET1-WI with early detection of blood-brain barrier changes on K(trans) maps and their role for prediction of subsequent hemorrhagic transformation in acute middle cerebral arterial infarct.

Methods: Twenty-six patients with acute middle cerebral arterial stroke and early spontaneous reperfusion, whose MR images were obtained at predetermined stroke stages, were included. T2*-based MR perfusion-weighted images were acquired using the first-pass pharmacokinetic model to derive K(trans) and vp. Parenchymal enhancement observed on maps of K(trans), vp, and CET1-WI at each stage was compared. Association among these measurements and hemorrhagic transformation was analyzed.

Results: K(trans) map showed significantly higher parenchymal enhancement in ischemic parenchyma as compared with that of vp map and CET1-WI at early stroke stages (P<0.05). The increased K(trans) at acute stage was not associated with parenchymal enhancement in CET1-WI at the same stage. Parenchymal enhancement in CET1-WI started to occur at the late subacute stage and tended to be luxury reperfusion-dependent. Patients with hemorrhagic transformation showed higher mean K(trans) values as compared with patients without hemorrhagic transformation (P=0.02).

Conclusions: Postischemic brain enhancement on routine CET1-WI seems to be closely related to the luxury reperfusion at the late subacute stage and is not dependent on microvascular permeability changes at the acute stage.
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http://dx.doi.org/10.1161/STROKEAHA.113.001558DOI Listing
July 2013

T2 values of posterior horns of knee menisci in asymptomatic subjects.

PLoS One 2013 28;8(3):e59769. Epub 2013 Mar 28.

Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan, ROC.

Purpose: The magnetic resonance (MR) T2 value of cartilage is a reliable indicator of tissue properties and therefore may be used as an objective diagnostic tool in early meniscal degeneration. The purpose of this study was to investigate age, gender, location, and zonal differences in MR T2 value of the posterior horns of knee menisci in asymptomatic subjects.

Methods: Sixty asymptomatic volunteers (30 men and 30 women) were enrolled and divided into three different age groups: 20-34, 35-49 and 50-70 years. The inclusion criteria were BMI<30 kg/cm(2), normalized Western Ontario and McMaster Universities (WOMAC) pain score of zero, and no evidence of meniscal and ligamentous abnormalities on routine knee MR imaging. The T2 values were measured on images acquired with a T2-weighted fat-suppressed turbo spin-echo sequence at 3T.

Results: The mean T2 values in both medial and lateral menisci for the 20-34, 35-49, and 50-70 age groups were 9.94 msec±0.94, 10.73 msec±1.55, and 12.36 msec±2.27, respectively, for women and 9.17 msec±0.74, 9.64 msec±0.67, and 10.95 msec±1.33, respectively, for men. The T2 values were significantly higher in the 50-70 age group than the 20-34 age group (P<0.001) and in women than in men (P = 0.001, 0.004, and 0.049 for each respective age group). T2 values were significantly higher in medial menisci than in lateral menisci only in women age 50-70 (3.33 msec, P = 0.006) and in the white zone and red/white zone of the 50-70 and 35-49 age groups than that of the 20-34 age group (2.47, 1.02; 2.77, 1.16 msec, respectively, all P<0.01).

Conclusion: The MR T2 values of the posterior meniscal horns increase with increasing age in women and are higher in women than in men. The age-related rise of T2 values appears to be more severe in medial menisci than in lateral menisci. Differences exist in the white zone and red/white zone.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059769PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610938PMC
January 2014

Altered TGF-β2 and bFGF expression in scleral desmocytes from an experimentally-induced myopia guinea pig model.

Graefes Arch Clin Exp Ophthalmol 2013 Apr 5;251(4):1133-44. Epub 2013 Feb 5.

Department of Ophthalmology, Bethune International Peace Hospital of PLA, Zhongshan Road No.398, Shijiazhuang, Hebei Province 050082, China.

Purpose: To determine changes in expression of transforming growth factor β-2 (TGF-β2) and basic fibroblast growth factor (bFGF) in scleral desmocytes from anterior and posterior portions of experimentally-induced myopic eyes of guinea pigs.

Methods: Three groups (n = 10) of 2-week-old guinea pigs were used to develop concave lens-induced myopia (LIM) in one eye via the out-of-focus method for 6, 15, or 30 days respectively, while the other eye in each guinea pig served as the self-control (SC). After myopia induction, lenses were removed, and scleral fibroblasts were cultured and passaged twice. TGF-β2 and bFGF expression levels of scleral desmocytes in LIM and SC groups were compared by immunocytochemistry, quantitative real-time PCR (qRT-PCR) and Western blot analyses.

Results: The TGF-β2 expression of the anterior portion of the sclera in the LIM group was significantly higher at 15 days, and at its highest at 30 days after myopia induction compared with the SC group (P < 0.05). The TGF-β2 staining of the posterior sclera in the LIM group began to rise significantly at 6 days, peaked at 15 days and remained significantly higher than that of the anterior part, as well as the SC group, even at 30 days after myopia induction (P < 0.05). BFGF levels in scleral desmocytes from the anterior and posterior regions in the LIM group were both significantly lower than those of the SC group at all time points after myopia induction (P < 0.05). Furthermore, as the myopia progressed, bFGF expression in the anterior and posterior sclera in the LIM group gradually and statistically significantly decreased compared with the SC group (P < 0.05); however, no significant differences were observed between the anterior and posterior parts in the LIM group at any time after myopia induction (P > 0.05). All these results were consistent at the mRNA and protein levels.

Conclusions: During myopia development in lens-induced guinea pigs, the increase in TGF-β2 activity of scleral desmocytes initiated at the posterior pole. Along with the induction time, the TGF-β2 activity in all scleral desmocytes became elevated. By contrast, the bFGF activity showed a general decline in all scleral desmocytes, rather than mainly in the posterior pole. These results imply that expression of TGF-β2 in scleral desmocytes plays a direct role, while that of bFGF exerts an indirect role in myopia development.
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http://dx.doi.org/10.1007/s00417-013-2269-8DOI Listing
April 2013

[Occupational health status of migrant female workers of artificial gem manufacturing cottages in Guangxi].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2011 Sep;29(9):646-9

Guangxi Prevention and Treatment Academy for Occupational Disease, Nanning, China.

Objective: To investigate occupational health status in migrant female workers in artificial gem cottages in Guangxi and to provide reference evidence for drawing up countermeasures to protect migrant female workers from occupational hazards.

Methods: Two thousands and ninety five migrant female workers in artificial gem manufacturing cottages in Wuzhou City, Guangxi Province, were randomly recruited to participate in the investigation. The standardized occupational health questionnaires were used to collect the information of demographic characteristics, occupational exposure and medical symptoms by interviewing with persons.

Results: Artificial gem manufacturing cottages in Wuzhou were mainly composed of family-workshops. There were only simple and crude hygienic facilities. Major occupational hazards included dusts, noise, vibration, hydrofluoric acid, and light stimulation. The mean age of workers was 30. Mean exposure duration was 5 years. There was no occupational health monitoring records for these workers in the past years. Major symptoms included back pain, eye discomfort, and headache. The incidence of discomfort symptoms increased with age.

Conclusion: Occupational health protection and monitoring in artificial gem manufacturing cottage, in Guangxi have not been performed. More effective occupational health education and administration need to implement in family-type handicraft manufacturing.
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http://dx.doi.org/10.3760/cma.j.issn.1001-9391.2011.09.003DOI Listing
September 2011

Mechanical behavior of scleral fibroblasts in experimental myopia.

Graefes Arch Clin Exp Ophthalmol 2012 Mar 19;250(3):341-8. Epub 2011 Nov 19.

The Department of Ophthalmology, The Second Hospital of Hebei Medical University, Heping Road No.125, Shijiazhuang, 050000, Hebei Province, China.

Background: The study aims to determine the changes in the biomechanical properties of the anterior and extreme posterior portions of experimental near-sighted eyes by examining the mechanical behavior of guinea pig scleral desmocytes, thus finding a new approach to the pathogenesis of myopia and their corresponding therapies.

Methods: Guinea pigs (2 weeks old) were numbered and assigned into three groups (A, B, and C) with ten guinea pigs each. Concave lens-induced myopic (LIM) animal models were prepared via the out-of-focus method. The other eye in the same guinea pig served as the self-control (SC) group. After modeling groups A, B, and C for 6, 15, and 30 days respectively, the lenses from the guinea pigs in the experimental group were removed. The scleral fibroblasts in each group were cultured, and passaged twice in vitro. The micropipette aspiration technique coupled with a viscoelastic solid model was utilized to investigate the viscoelastic properties of the scleral fibroblasts in normal and myopic guinea pigs. The mechanical behavior of the scleral desmocytes of the LIM and SC groups were compared.

Results: The mechanical behavior of the scleral desmocytes was compared between the LIM and SC groups. The Young's modulus at equilibrium and the apparent cellular viscosity of the anterior portion of the sclera in the LIM group at 6 days and 15 days after myopic induction were not significantly different from that of the SC group (P < 0.05). However, the results for the anterior portions of the sclera in the LIM group at 30 days were significantly higher than those of the LIM group at 6 and 15 days, as well as those in the SC group (P < 0.05). The Young's modulus at equilibrium and the apparent cellular viscosity of the extreme posterior portions of the sclera in the LIM group at 6 days after myopic induction not significantly from those of the SC group (P < 0.05). However, the results for the extreme posterior portions of the sclera in the LIM group after 15 days and 30 days were significantly higher than those in the LIM group at 6 days and the SC group (P < 0.05).

Conclusions: The Young's modulus at equilibrium or apparent cellular viscosity of all the anterior portions of the sclera in the LIM group were longer than those in the SC group at 30 days after the induction, and the results for all the extreme posterior portions of the LIM group were larger than those of the SC group on the 15th and 30th day. Therefore, the Young's modulus and apparent viscosity of the anterior and extreme posterior portions of the sclera changed on the 15th and 30th day after induction respectively.
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http://dx.doi.org/10.1007/s00417-011-1854-yDOI Listing
March 2012

Potential long-term effects of MDMA on the basal ganglia-thalamocortical circuit: a proton MR spectroscopy and diffusion-tensor imaging study.

Radiology 2011 Aug 1;260(2):531-40. Epub 2011 Jun 1.

Department of Radiology, Tri-Service General Hospital and National Defense Medical Center, 325 Cheng-Kung Rd, NeiHu, Taipei, Taiwan 114, Republic of China.

Purpose: To investigate the effects of 3,4-methylenedioxymethamphetamine (MDMA, commonly known as "ecstasy") on the alterations of brain metabolites and anatomic tissue integrity related to the function of the basal ganglia-thalamocortical circuit by using proton magnetic resonance (MR) spectroscopy and diffusion-tensor MR imaging.

Materials And Methods: This study was approved by a local institutional review board, and written informed consent was obtained from all subjects. Thirty-one long-term (>1 year) MDMA users and 33 healthy subjects were enrolled. Proton MR spectroscopy from the middle frontal cortex and bilateral basal ganglia and whole-brain diffusion-tensor MR imaging were performed with a 3.0-T system. Absolute concentrations of metabolites were computed, and diffusion-tensor data were registered to the International Consortium for Brain Mapping template to facilitate voxel-based group comparison.

Results: The mean myo-inositol level in the basal ganglia of MDMA users (left: 4.55 mmol/L ± 2.01 [standard deviation], right: 4.48 mmol/L ± 1.33) was significantly higher than that in control subjects (left: 3.25 mmol/L ± 1.30, right: 3.31 mmol/L ± 1.19) (P < .001). Cumulative lifetime MDMA dose showed a positive correlation with the levels of choline-containing compounds (Cho) in the right basal ganglia (r = 0.47, P = .02). MDMA users also showed a significant increase in fractional anisotropy (FA) in the bilateral thalami and significant changes in water diffusion in several regions related to the basal ganglia-thalamocortical circuit as compared with control subjects (P < .05; cluster size, >50 voxels).

Conclusion: Increased myo-inositol and Cho concentrations in the basal ganglia of MDMA users are suggestive of glial response to degenerating serotonergic functions. The abnormal metabolic changes in the basal ganglia may consequently affect the inhibitory effect of the basal ganglia to the thalamus, as suggested by the increased FA in the thalamus and abnormal changes in water diffusion in the corresponding basal ganglia-thalamocortical circuit.
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http://dx.doi.org/10.1148/radiol.11101918DOI Listing
August 2011

Idiopathic growth hormone deficiency in the morphologically normal pituitary gland is associated with perfusion delay.

Radiology 2011 Jan 30;258(1):213-21. Epub 2010 Sep 30.

Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan.

Purpose: To investigate quantitatively the topographic perfusion characteristics of the adenohypophysis by using dynamic contrast material-enhanced magnetic resonance (MR) imaging in a subgroup of patients with idiopathic growth hormone deficiency (IGHD) and with normal-appearing pituitary morphology on MR images.

Materials And Methods: This HIPAA-compliant, prospective study was approved by an institutional review board, and informed consent was obtained for all patients. Twenty-five patients (mean age, 10.6 years ± 3.3 [standard deviation]) with clinical growth retardation, proved IGHD, and normal pituitary morphology on MR images were included for analysis. Sixteen children (mean age, 10.8 years ± 5.5) were included as control subjects. Time to peak (TTP) perfusion properties of the adenohypophysis in 10 regions of interest from multisection coronal dynamic contrast-enhanced T1-weighted MR images were quantitatively derived by using the Brix pharmacokinetic model. Significant difference was determined with a two-tailed Student t test. The Pearson correlation coefficient was used to correlate the perfusion parameters, including maximal enhancement peak and slope, with serum growth hormone levels in the IGHD group.

Results: TTP for the IGHD group was significantly prolonged compared with that for the control group (P < .005). The prolonged TTP in the IGHD group was found to be diffuse. The levels of growth hormone deficiency were negatively correlated with the peak enhancement and the slope of the wash-in phase, which suggests increased blood volume in IGHD within the pituitary gland.

Conclusion: IGHD and the degree of growth hormone deficiency are associated with nonregional perfusion delay in morphologically normal adenohypophyses. The lack of lateralization of perfusion delay may suggest that microvascular structural abnormalities play a role in IGHD.
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http://dx.doi.org/10.1148/radiol.10100504DOI Listing
January 2011

T2-enhanced tensor diffusion trace-weighted image in the detection of hyper-acute cerebral infarction: comparison with isotropic diffusion-weighted image.

Eur J Radiol 2010 Jun 9;74(3):e89-94. Epub 2009 May 9.

Department of Radiology, Tri-Service General Hospital, Taipei, Taiwan, ROC.

Background And Purpose: Although isotropic diffusion-weighted imaging (isoDWI) is very sensitive to the detection of acute ischemic stroke, it may occasionally show diffusion negative result in hyper-acute stroke. We hypothesize that high diffusion contrast diffusion trace-weighted image with enhanced T2 may improve stroke lesion conspicuity.

Methods: Five hyper acute stroke patients (M:F=0:5, average age=61.8+/-20.5 y/o) and 16 acute stroke patients (M:F=11:5, average age=67.7+/-12 y/o) were examined six-direction tensor DWIs at b=707s/mm(2). Three different diffusion-weighted images, including isotropic (isoDWI), diffusion trace-weighted image (trDWI) and T2-enhanced diffusion trace-weighted image (T2E_trDWI), were generated. Normalized lesion-to-normal ratio (nLNR) and contrast-to-noise ratio (CNR) of three diffusion images were calculated from each patient and statistically compared.

Results: The trDWI shows better nLNR than isoDWI on both hyper-acute and acute stroke lesions, whereas no significant improvement in CNR. Nevertheless, the T2E_trDWI has statistically superior CNR and nLNR than those of isoDWI and trDWI in both hyper-acute and acute stroke.

Conclusions: We concluded that tensor diffusion trace-weighted image with T2 enhancement is more sensitive to stroke lesion detection, and can provide higher lesion conspicuity than the conventional isotropic DWI for early stroke lesion delineation without the need of high-b-value technique.
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http://dx.doi.org/10.1016/j.ejrad.2009.04.023DOI Listing
June 2010

Perfusion characteristics of late radiation injury of parotid glands: quantitative evaluation with dynamic contrast-enhanced MRI.

Eur Radiol 2009 Jan 26;19(1):94-102. Epub 2008 Jul 26.

Department of Radiology, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan, Republic of China.

We aimed to quantitatively investigate the alteration of parotid perfusion after irradiation using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) based on a two-compartment tracer kinetic model. This study enrolled 19 patients (53.2 +/- 14.9 years) treated by head and neck radiotherapy and 19 age-relevant and sex-matched subjects as a control group. Perfusion parameters (K ( el ), k (21) and A) of parotid glands were analyzed based on the Brix model from T1-weighted DCE-MRI. Suitability of the Brix model was evaluated via Monte Carlo simulation for the goodness-of-fit. Analysis of nonlinear goodness-of-fit showed that the Brix model is appropriate in evaluating the parotid perfusion (R(2) = 0.938 +/- 0.050). The irradiated parotid glands showed significantly lower K ( el ) (P < 0.0005) and k (21) (P < 0.05) and consequently significantly higher value of peak enhancement (P < 0.0005) and time-to-peak (P < 0.0005) compared with non-irradiated ones, suggestive of gradual and prolonged accumulation and delayed wash-out of contrast agent due to increased extracellular extravascular space and decreased vascular permeability in the irradiated glands. Linear regression analysis showed dose-dependent perfusion changes of the irradiated parotid glands. We conclude that quantitative DCE-MRI is a potential tool in investigating parotid gland perfusion changes after radiotherapy.
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http://dx.doi.org/10.1007/s00330-008-1104-9DOI Listing
January 2009

[Survey of current condition of occupational hygiene technology service organizations in Guangxi Province].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2008 Feb;26(2):104-6

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February 2008

Tensor deflection (TEND) tractography with adaptive subvoxel stepping.

J Magn Reson Imaging 2006 Aug;24(2):451-8

Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.

Purpose: To develop an adaptive subvoxel stepping scheme, as an adjunct to tensor deflection (TEND) tractography, that automatically adjusts the stepping size by considering the tensor linearity to properly trace fiber bundles in regions with different degrees of tensor anisotropy.

Materials And Methods: A theoretical investigation of the TEND algorithm was performed to assess the degree of deflection of the propagation vector toward the major eigenvector. Mathematically generated phantoms (one with curved fibers and the other with crossing fibers) at wide ranges of signal-to-noise ratio (SNR), and human brain images obtained in vivo were used to test the performance of the adaptive stepping algorithm.

Results: The degree of deflection was found to be inversely related to the stepping size. A small stepping size was advantageous for tracing single curved fiber bundles, whereas a large stepping size was beneficial for passing through fiber crossing regions. The performance of the adaptive stepping algorithm was superior to fixed stepping in both situations, leading to an approximately 0.17 voxel of deviation in curved fibers and a nearly 100% successful tracking rate in crossing fibers at typical SNR. Human brain images demonstrated similar results.

Conclusion: The adaptive stepping algorithm is a helpful adjunct to TEND tractography.
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http://dx.doi.org/10.1002/jmri.20652DOI Listing
August 2006

FLAIR diffusion-tensor MR tractography: comparison of fiber tracking with conventional imaging.

AJNR Am J Neuroradiol 2005 Mar;26(3):591-7

Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan, ROC.

Background And Purpose: Partial volume with CSF is known to contaminate the quantification of white matter anisotropy depicted by diffusion tensor imaging (DTI). We hypothesized that the FLAIR technique helps to improve DTI white matter tractography in the normal adult brain by eliminating CSF partial volume effects.

Methods: Seven healthy adults aged 23-37 underwent both conventional and FLAIR DTI at 1.5T. Each subject was imaged five times. Neural fiber tractography was performed with both sequences by using two algorithms: a voxel-based method (EZ-tracing) with global seed points and another based on subvoxel tractography (tensor deflection) by using manual encircling of local seed points. Total volume of the fibers tracked was compared for the two types of images.

Results: Fiber tracking was substantially most successful on FLAIR DTI near the lateral ventricles and the sulci, where CSF partial volume effects were likely present. Minor false tracts on FLAIR images, possibly due to a reduced signal-to-noise ratio, were found in regions relatively free of CSF contamination; however, they did not affect tracking of major periventricular white matter bundles, such as those related to the corpus callosum or the corona radiata. When we excluded false tracts, the FLAIR technique depicted an average of 17% more fibers in volume than conventional DTI in the periventricular regions (P < .0005, paired Student t test).

Conclusion: Despite the reduction of signal-to-noise ratio and longer imaging times, FLAIR improved tractography by eliminating CSF partial volume effects.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976478PMC
March 2005

[Distribution of hepatitis B virus genotypes and its clinical significance in Guangxi].

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2003 Jun;17(2):174-9

Guangxi Institute of Occupational Health, Nanning, Guangxi 530021, China.

Objective: To understand the distribution of hepatitis B virus genotype in Guangxi and its clinical significance.

Methods: Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA in sera of asymptomatic carrier (ASC) of hepatitis B virus (HBV) and patients with different liver diseases from southern and northern Guangxi. Specimens from 161 subjects were positive for HBV DNA and HBV genotype was determined by using restriction fragment length polymorphism analysis, direct sequencing or cloning sequencing.

Results: The prevalence of genotype A was 3.7% in all samples and that of genotype B, C and D was 21.7%, 72.7% and 1.2%, respectively. No other genotypes (such as genotype E, F, G, H) were found. The prevalence of genotype C showed an increasing trend in ASC, chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC) group; in contrast, the prevalence of genotype B showed an opposite trend, although no statistically significant difference was observed, except between ASC and HCC (P=0.05). The HBeAg positive rate was higher, and the anti-HBe positive rate was lower in patients with chronic genotype C infection than in those with genotype B (P<0.05 for both). Liver function test (ALT) abnormality was more severe in genotype C group than in genotypes A and B groups having acute or chronic infection (P<0.01 for all comparisons). The prevalence of genotype C in southern Guangxi was higher than that in northern Guangxi. In contrast, the prevalence of genotype B in southern Guangxi was lower than that in northern Guangxi.

Conclusions: 1. The predominant HBV genotypes in Guangxi were genotypes B and C. The major genotype in southern Guangxi was genotype C; while that in northern Guangxi was genotype B, which implied that the distribution of HBV genotype C was consistent with the incidence of HCC in Guangxi. 2. Genotype C maybe associated with development of severe liver diseases including HCC. 3. Genotype A,D and B+C were mostly found in acute, hepatitis and chronic hepatitis group.
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June 2003

[Determination of trace chrysene in environmental water by solid-phase microextraction coupled with high performance liquid chromatography].

Se Pu 2002 Jan;20(1):59-62

School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.

A rapid method for the determination of trace chrysene in environmental water by solid-phase microextraction (SPME) coupled with high performmnce liquid chromatography (HPLC) was developed. The experimental conditions of SPME, such as extraction time, extraction temperature, effect of ion strength, desorption time, desorption solution, desorption mode and the analytical conditions of HPLC were optimized. The optimal conditions were room temperature, 1,100 r/min of stirring rate, 30 min of extraction time, 3 min of desorption time, and methanol as the desorption solution. Methanol was used as the mobile phase on a C18 reversed phase chromatographic column. The flow rate was 1 mL/min. The detection wavelength was 266 nm on a UV-Vis detector. The linear range was from 0.013 microgram/L to 3.0 micrograms/L, the detection limit was 2.7 ng/L, and the relative standard deviation (RSD) was 5.6%. The method was used for the determination of trace chrysene in tap water, mineral water, rain water and river water. The recoveries were from 103.2% to 119.3%, the RSDs were from 4.8% to 10.2%. The method is fast, convenient, sensitive, solvent-free, and suitable for the determination of trace chrysene in environmental water.
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January 2002
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