Publications by authors named "Chao Yang"

1,558 Publications

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Neutrophil extracellular traps impair intestinal barrier functions in sepsis by regulating TLR9-mediated endoplasmic reticulum stress pathway.

Cell Death Dis 2021 Jun 11;12(6):606. Epub 2021 Jun 11.

Division of Trauma and Surgical Intensive Care Unit, Research Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu, P. R. China.

Increased neutrophil extracellular traps (NETs) formation has been found to be associated with intestinal inflammation, and it has been reported that NETs may drive the progression of gut dysregulation in sepsis. However, the biological function and regulation of NETs in sepsis-induced intestinal barrier dysfunction are not yet fully understood. First, we found that both circulating biomarkers of NETs and local NETs infiltration in the intestine were significantly increased and had positive correlations with markers of enterocyte injury in abdominal sepsis patients. Moreover, the levels of local citrullinated histone 3 (Cit H3) expression were associated with the levels of BIP expression. To further confirm the role of NETs in sepsis-induced intestinal injury, we compared peptidylarginine deiminase 4 (PAD4)-deficient mice and wild-type (WT) mice in a lethal septic shock model. In WT mice, the Cit H3-DNA complex was markedly increased, and elevated intestinal inflammation and endoplasmic reticulum (ER) stress activation were also found. Furthermore, PAD4 deficiency alleviated intestinal barrier disruption and decreased ER stress activation. Notably, NETs treatment induced intestinal epithelial monolayer barrier disruption and ER stress activation in a dose-dependent manner in vitro, and ER stress inhibition markedly attenuated intestinal apoptosis and tight junction injury. Finally, TLR9 antagonist administration significantly abrogated NETs-induced intestinal epithelial cell death through ER stress inhibition. Our results indicated that NETs could contribute to sepsis-induced intestinal barrier dysfunction by promoting inflammation and apoptosis. Suppression of the TLR9-ER stress signaling pathway can ameliorate NETs-induced intestinal epithelial cell death.
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http://dx.doi.org/10.1038/s41419-021-03896-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195983PMC
June 2021

A "double-locked" probe for the detection of hydrogen sulfide in a viscous system.

Chem Commun (Camb) 2021 Jun 11. Epub 2021 Jun 11.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

A novel "double-locked" probe, DCO-H2S-V, was prepared for detecting hydrogen sulfide in a highly viscous system. Experiments demonstrated that only when H2S and a high viscosity environment coexist in a cell, can the probe be activated effectively and emit fluorescence. This has been successfully used for detecting the changes in viscosity and H2S in a Parkinson's disease model, PC-12 cells treated with glutamate.
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http://dx.doi.org/10.1039/d1cc01819aDOI Listing
June 2021

Curcumin Alleviates Cerebral Ischemia-reperfusion Injury by Inhibiting NLRP1-dependent Neuronal Pyroptosis.

Curr Neurovasc Res 2021 Jun 7. Epub 2021 Jun 7.

Department of Neurosurgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.

Background: Cerebral ischemia-reperfusion injury is caused by a blood reperfusion injury in the ischemic brain and usually occurs in the treatment stage of ischemic disease, which can aggravate brain tissue injury.

Objective: Curcumin was reported to exert a good therapeutic effect on neural cells against ischemia-reperfusion injury, while the mechanism was unclear.

Methods: In this study, oxygen glucose deprivation (OGD) model of fetal rat cerebral cortical neurons and the middle cerebral artery occlusion (MCAO) model of rats were employed to mimic cerebral ischemia-reperfusion injury in vitro and in vivo,respectively.

Results: We confirmed that curcumin has a promotive effect on neuronal proliferation and an inhibitory effect on neuronal pyroptosis. Furthermore, we found that curcumin could improve cerebral infarction. The results of western blotting showed that curcumin down-regulated the expression of nucleotide-binding oligomerization domain-containing protein-, leucine-rich repeats-, and pyrin domain-containing protein 1 (NLRP1), cysteinyl aspartate-specific protease 1 (caspase-1), gasdermin D (GSDMD), IL-1β, IL-6, TNF-α, and iNOS proteins in OGD and MCAO models. NLRP1-dependent neuronal pyroptosis played an important role in cerebral ischemia-reperfusion injury.

Conclusion: Curcumin could effectively inhibit NLRP1-dependent neuronal pyroptosis by suppressing the p38 MAPK pathway and therefore exerted neuroprotective effects against cerebral ischemia-reperfusion injury.
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http://dx.doi.org/10.2174/1567202618666210607150140DOI Listing
June 2021

Low-Molecular-Weight Poly(ethylenimine) Nanogels Loaded with Ultrasmall Iron Oxide Nanoparticles for -Weighted MR Imaging-Guided Gene Therapy of Sarcoma.

ACS Appl Mater Interfaces 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory for Modification of Chemical Fiber and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.

Cancer metastasis is still a major obstacle in clinical cancer therapy and a paramount cause of cancer deaths. Designing multifunctional nanoplatforms with an enhanced diagnostic sensitivity and anti-metastasis efficiency against tumors represents a major trend in current cancer management. Herein, we report the preparation of low-molecular-weight poly(ethylenimine) (PEI)-poly(ethylene glycol) (PEG) nanogels (NGs) loaded with transforming growth factor-β1 (TGF-β1) siRNA and ultrasmall iron oxide nanoparticles (FeO NPs) for gene therapy and -weighted magnetic resonance (MR) imaging of tumors and tumor metastasis in a mouse sarcoma model. In this work, ultrasmall FeO NPs stabilized by sodium citrate were first prepared and then mixed with PEI (800 Da) and PEG (400 Da)-diacrylate as a cross-linker to form FeO/PEI-PEG NGs with an average size of 76.3 nm an inverse microemulsion method. The developed hybrid NGs display good cytocompatibility and enhanced MR imaging performance ( relaxivity = 1.0346 mM s). The FeO/PEI-PEG NGs can be further used to compact TGF-β1 siRNA through electrostatic interaction and efficiently deliver siRNA to cancer cells and a tumor model to silence the TGF-β1 gene, which inhibits the growth and invasion of cancer cell significantly, as well as the growth of a subcutaneous sarcoma tumor model and lung metastasis . The designed hybrid NG-ultrasmall iron oxide NPs may be extended for the delivery of other drugs or genes for theranostics of different biological systems.
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http://dx.doi.org/10.1021/acsami.1c04081DOI Listing
June 2021

Robust, Multiresponsive, Superhydrophobic, and Oleophobic Nanocomposites via a Highly Efficient Multifluorination Strategy.

ACS Appl Mater Interfaces 2021 Jun 9. Epub 2021 Jun 9.

Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

Artificial superhydrophobic surfaces are garnering constant attention due to their wide applications. However, it is a great challenge for superhydrophobic materials to simultaneously achieve good oil repellency, mechanochemical robustness, adhesion, thermomechanical properties, and multiresponsive ability. Herein, we propose a highly efficient multifluorination strategy to prepare superhydrophobic nanocomposites with the above features, which can be used as monoliths or coatings on various substrates. In this strategy, long-chain perfluorinated epoxy (PFEP) provides outstanding water/oil repellency, tetrafluorophenyl-based epoxy (FEP) possesses good thermodynamic compatibility with PFEP and increases the mechanical performance of the matrix, and carbon nanotubes grafted with perfluorinated segments and flexible spacers (FCNTs) tailor the surface roughness as well as impart multiple functions and ensure good binding interfaces. Notedly, all of the applications of constrained long-chain perfluorinated compounds are achieved via thiol-ene click chemistry, following the ethos of atom economy. The resultant PFEP/FCNTs exhibits superhydrophobicity and oleophobicity, thermal conductivity (1.33 W·m·K), electronic conductivity (232 S m), and electromagnetic interference shielding properties (∼30 dB at 8.2-12.4 GHz, 200 μm). Importantly, after different extreme physical/chemical tests, the PFEP/FCNTs coating still shows outstanding water/oil repellency. In addition, the coating exhibits good photo/electrothermal conversion ability and shows the potential for sensor application. Moreover, the novel strategy provides an efficient guideline for large-scale preparation of robust, multiresponsive, superhydrophobic, and oleophobic materials.
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http://dx.doi.org/10.1021/acsami.1c07048DOI Listing
June 2021

A small molecule HIF-1α stabilizer that accelerates diabetic wound healing.

Nat Commun 2021 06 7;12(1):3363. Epub 2021 Jun 7.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

Impaired wound healing and ulcer complications are a leading cause of death in diabetic patients. In this study, we report the design and synthesis of a cyclometalated iridium(III) metal complex 1a as a stabilizer of hypoxia-inducible factor-1α (HIF-1α). In vitro biophysical and cellular analyses demonstrate that this compound binds to Von Hippel-Lindau (VHL) and inhibits the VHL-HIF-1α interaction. Furthermore, the compound accumulates HIF-1α levels in cellulo and activates HIF-1α mediated gene expression, including VEGF, GLUT1, and EPO. In in vivo mouse models, the compound significantly accelerates wound closure in both normal and diabetic mice, with a greater effect being observed in the diabetic group. We also demonstrate that HIF-1α driven genes related to wound healing (i.e. HSP-90, VEGFR-1, SDF-1, SCF, and Tie-2) are increased in the wound tissue of 1a-treated diabetic mice (including, db/db, HFD/STZ and STZ models). Our study demonstrates a small molecule stabilizer of HIF-1α as a promising therapeutic agent for wound healing, and, more importantly, validates the feasibility of treating diabetic wounds by blocking the VHL and HIF-1α interaction.
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http://dx.doi.org/10.1038/s41467-021-23448-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184911PMC
June 2021

Bioelectrical impedance analysis-guided fluid management promotes primary fascial closure after open abdomen: a randomized controlled trial.

Mil Med Res 2021 Jun 7;8(1):36. Epub 2021 Jun 7.

Division of Trauma and Surgical Intensive Care Unit, Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, No. 305 East Zhongshan Road, Nanjing, 210002, Jiangsu, China.

Background: Fluid overload (FO) after resuscitation is frequent and contributes to adverse outcomes among postinjury open abdomen (OA) patients. Bioelectrical impedance analysis (BIA) is a promising tool for monitoring fluid status and FO. Therefore, we sought to investigate the efficacy of BIA-directed fluid resuscitation among OA patients.

Methods: A pragmatic, prospective, randomized, observer-blind, single-center trial was performed for all trauma patients requiring OA between January 2013 and December 2017 to a national referral center. A total of 140 postinjury OA patients were randomly assigned in a 1:1 ratio to receive either a BIA-directed fluid resuscitation (BIA) protocol that included fluid administration with monitoring of hemodynamic parameters and different degrees of interventions to achieve a negative fluid balance targeting the hydration level (HL) measured by BIA or a traditional fluid resuscitation (TRD) in which clinicians determined the fluid resuscitation regimen according to traditional parameters during 30 days of ICU management. The primary outcome was the 30-day primary fascial closure (PFC) rate. The secondary outcomes included the time to PFC, postoperative 7-day cumulative fluid balance (CFB) and adverse events within 30 days after OA. The Kaplan-Meier method and the log-rank test were utilized for PFC after OA. A generalized linear regression model for the time to PFC and CFB was built.

Results: A total of 134 patients completed the trial (BIA, n = 66; TRD, n = 68). The BIA patients were significantly more likely to achieve PFC than the TRD patients (83.33% vs. 55.88%, P < 0.001). In the BIA group, the time to PFC occurred earlier than that of the TRD group by an average of 3.66 days (P < 0.001). Additionally, the BIA group showed a lower postoperative 7-day CFB by an average of 6632.80 ml (P < 0.001) and fewer complications.

Conclusion: Among postinjury OA patients in the ICU, the use of BIA-guided fluid resuscitation resulted in a higher PFC rate and fewer severe complications than the traditional fluid resuscitation strategy.
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http://dx.doi.org/10.1186/s40779-021-00329-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180439PMC
June 2021

Optimized Propofol Anesthesia Increases Power of Subthalamic Neuronal Activity in Patients with Parkinson's Disease Undergoing Deep Brain Stimulation.

Neurol Ther 2021 Jun 6. Epub 2021 Jun 6.

Department of Neurosurgery, First Affiliated Hospital of Sun Yat-sen University , Guangzhou, 510080, Guangdong Province, People's Republic of China.

Introduction: Propofol is a general anesthetic option for deep brain stimulation (DBS) of the subthalamic nucleus (STN) of patients with Parkinson's disease (PD). However, its effects on STN activity and neuropsychological outcomes are controversial. The optimal propofol anesthesia for asleep DBS is unknown. This study investigated the safety and effectiveness of an optimized propofol anesthesia regimen in asleep DBS.

Methods: This retrospective study enrolled 68 PD patients undergoing bilateral STN-DBS surgery. All patients received local scalp anesthesia, with (asleep group, n = 35) or without (awake group, n = 33) propofol-remifentanil general anesthesia by target-controlled infusion under electroencephalogram monitoring. The primary outcome was subthalamic neuronal spiking characterization during microelectrode recording. The secondary outcomes were clinical outcomes including motor, cognition, mind, sleep, and quality of life at 6 months.

Results: Significantly increased delta and theta power were obtained under propofol anesthesia (awake vs. asleep group, mean ± standard deviation; delta: 31.97 ± 9.87 vs. 39.77 ± 10.56, p < 0.01; theta: 21.09 ± 5.55 vs. 24.82 ± 6.63, p = 0.01). After excluding the influence of confounding factors of age and preoperative motor scores, there was a statistically significant influence on the delta, theta, and alpha power of STN neuronal activity under different anesthesia regimens (delta: β = 2.64, p < 0.01; theta: β = 2.11, p < 0.01; alpha: β = 1.42, p = 0.01). There were no differences in modified burst index, firing rate, tract numbers of microelectrode recording, and other clinical outcomes between the two groups.

Conclusion: Optimized propofol anesthesia enhanced the delta, theta, and alpha power in STN compared with the awake technique and likely contributed to target recognition under propofol anesthesia. These results demonstrate that propofol is suitable, but needs to be optimized, for asleep STN-DBS.

Trial Registration: Chinese Clinical Trial Registry Identification number: ChiCTR2100045942. Registered 29 April 2021-Retrospectively registered.
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http://dx.doi.org/10.1007/s40120-021-00259-yDOI Listing
June 2021

How Experiences Affect Psychological Responses During Supervised Fasting: A Preliminary Study.

Front Psychol 2021 19;12:651760. Epub 2021 May 19.

State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.

As an unusual event, fasting can induce strong physiological and psychological reactions, but there is still no clear understanding of how previous fasting experiences affect people's responses to current fasting. This study aimed to investigate the influence of previous fasting experiences on participants' basic physiological and psychological responses in a fasting experiment conducted under intensive medical monitoring. For a 22-day experiment divided into four phases (3-day Baseline; 10-day Complete Fasting; 4-day Calorie Restriction; and 5-day Recovery phases), a total of 13 persons participated; the participants were divided into a group with prior fasting experience (Experienced: = 6) and a group without prior fasting experience (Newbie: = 7). The results indicate no group differences in physiological responses (i.e., weight, glucose, and ketone bodies); however, differences in psychological states were observed, with the Newbie group showing more negative psychological states overall throughout the experiment (i.e., greater appetite, more negative mood states, more stress, less vitality, and fewer recovery resources). Hence, previous fasting experience may be a buffer against negative feelings during current fasting. For this reason, it is important to consider fasting experiences as a vital factor in future research.
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http://dx.doi.org/10.3389/fpsyg.2021.651760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170085PMC
May 2021

Efficient carboxylation of styrene and carbon dioxide by single-atomic copper electrocatalyst.

J Colloid Interface Sci 2021 May 23;601:378-384. Epub 2021 May 23.

Laboratory of Advanced Materials, Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Faculty of Chemistry and Materials Science, Fudan University, Shanghai 200438, China. Electronic address:

Electrocarboxylation of olefins with carbon dioxide (CO) is a potential approach to produce carboxylates as synthetic intermediates of polymer and pharmaceuticals. Nonetheless, due to the intrinsic inertness of CO at ambient conditions, the electrocarboxylation efficiency has been quite limited, typically with high applied potentials and low current densities. In this work, we demonstrate that nitrogen-coordinated single-atomic copper sites on carbon framework (Cu/NC) served as an excellent electrocatalyst for electrocarboxylation of styrene with CO. The Cu/NC catalyst allowed to efficiently activate CO, followed by nucleophilic attack to carboxylate styrene to produce phenylsuccinic acid, thus leading the reaction toward the CO activation pathway. The enhanced CO activation capability enabled increased selectivity and activity for electrocarboxylation of styrene. The Faradaic efficiency of electrocarboxylation was 92%, suggesting most of the activated CO proceeded to react with styrene rather than direct reduction to CO or CH. The electrocarboxylation exhibited almost 100% product selectivity toward phenylsuccinic acid, with a high partial current density of 58 mA·cm at -2.2 V (vs. Ag/AgI), corresponding to an outstanding production rate of 216 mg·cm·h, substantially exceeding previously reported works. Our work suggests an exciting perspective in electrocarboxylation of olefins by rational design of CO activation electrocatalysts.
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http://dx.doi.org/10.1016/j.jcis.2021.05.105DOI Listing
May 2021

Coordination and Redox Dual-Responsive Mesoporous Organosilica Nanoparticles Amplify Immunogenic Cell Death for Cancer Chemoimmunotherapy.

Small 2021 Jun 3:e2100006. Epub 2021 Jun 3.

Institutes for Life Sciences, School of Medicine, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong, 510006, China.

Amplifying the chemotherapy-driven immunogenic cell death (ICD) for efficient and safe cancer chemoimmunotherapy remains a challenge. Here, a potential ICD nanoamplifier containing diselenide-bridged mesoporous organosilica nanoparticles (MONs) and chemotherapeutic ruthenium compound (KP1339) to achieve cancer chemoimmunotherapy is tailored. KP1339-loaded MONs show controlled drug release profiles via glutathione (GSH)-responsive competitive coordination and matrix degradation. High concentration of MONs selectively evoked reactive oxygen species production, GSH depletion, and endoplasmic reticulum stress in cancer cells, thus amplifying the ICD of KP1339 and boosting robust antitumor immunological responses. After the combination of PD-L1 checkpoint blockade, cancer cell membrane-cloaked KP1339-loaded MONs not only regress primary tumor growth with low systemic toxicity, but also inhibit distant tumor growth and pulmonary metastasis of breast cancer. The results have shown the potential of coordination and redox dual-responsive MONs boosting amplified ICD for cancer chemoimmunotherapy.
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http://dx.doi.org/10.1002/smll.202100006DOI Listing
June 2021

Soil degradation influences soil bacterial and fungal community diversity in overgrazed alpine meadows of the Qinghai-Tibet Plateau.

Sci Rep 2021 Jun 2;11(1):11538. Epub 2021 Jun 2.

Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China.

Over half of the alpine meadows in the Qinghai-Tibet Plateau (QTP) are degraded due to human activities. Soil degradation from overgrazing is the most direct cause of grassland degradation. It is thus important to synthesize the effects of multiple soil degradation indicators on the belowground biomass of plants and soil microorganisms in the degraded QTP. We studied the diversities and structures of soil bacterial and fungal communities using soil bacterial 16S rRNA and the fungal ITS gene under four degradation gradients, D1: lightly degraded, D2: moderately degraded, D3: highly degraded, and a non-degraded control site (CK). The bacterial Shannon diversity in D3 was significantly lower than that in D1 (p < 0.001), and the bacterial richness index in D3 was significantly lower than that in D1 (p < 0.001). There was no difference in soil fungal diversity among the different degradation levels; however, soil fungal richness decreased significantly from CK to D3. The phyla Actinobacteria, Acidobacteria and the genus Mortierella were differed significantly under the four degradation gradients. Plant litter mass and root C/N ratio were important factors associated with bacterial and fungal diversity and richness. These results indicated that alpine meadow degradation can lead to variations in both microbial diversity and the potential functioning of micro-organisms in the QTP.
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http://dx.doi.org/10.1038/s41598-021-91182-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172827PMC
June 2021

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing.

Biomolecules 2021 May 8;11(5). Epub 2021 May 8.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.
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http://dx.doi.org/10.3390/biom11050702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151889PMC
May 2021

Classical swine fever virus infection suppresses claudin-1 expression to facilitate its replication in PK-15 cells.

Microb Pathog 2021 Aug 29;157:105012. Epub 2021 May 29.

College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, Yunnan, China. Electronic address:

Classical swine fever (CSF) is one of the most epidemic viral diseases in swine industry. The causative pathogen is CSF virus (CSFV), a small enveloped RNA virus of Flaviviridae family. Claudin-1 was reported to be involved in the infections of a number of viruses, including many from Flaviviridae family, but no studies have investigated the role of porcine claudin-1 during CSFV infection in PK-15 cells. In this study, on the one hand, we demonstrated that CSFV infection reduced the claudin-1 expression at both mRNA and protein levels; on the other hand, CSFV infection was enhanced after claudin-1 knockdown, but inhibited by claudin-1 overexpression in a dose-dependent manner. Furthermore, negative correlation was demonstrated between the claudin-1 expression and CSFV titer. In conclusion, claudin-1 might be a barrier for CSFV infection in PK-15 cells, while CSFV bypasses the barrier through lysosome mediated degradation of claudin-1, which could be repressed by bafilomycin A1. Although the elaborate mechanisms how claudin-1 plays its roles in CSFV infection require further investigations, this study may advance our understanding of the molecular host-pathogen interaction mechanisms underlying CSFV infection and suggests enhancement of porcine claudin-1 as a potential preventive or therapeutic strategy for CSF control.
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http://dx.doi.org/10.1016/j.micpath.2021.105012DOI Listing
August 2021

Scalable fabrication of crystalline COF membrane from amorphous polymeric membrane.

Angew Chem Int Ed Engl 2021 Jun 1. Epub 2021 Jun 1.

Tianjin University, School of Chemical Engineering and Technology, Weijin Road, 300072, Tianjin, CHINA.

Covalent organic framework (COF) membranes hold tremendous potential for widespread applicability, but their scalable fabrication remains challenging. Here, we demonstrate the disorder-to-order transformation from amorphous polymeric membrane to crystalline COF membrane via monomer exchange. Solution-processing method is used to prepare amorphous membranes and the replacing monomer is selected based on the chemical and thermodynamical stability of the final framework. Afterwards, the reversible imine bonds allow the extraneous monomers to replace the pristine monomers within amorphous membrane, driving the transformation from disordered network to ordered framework. The incorporation of intramolecular hydrogen bonds enables the crystalline COF to imprint the morphology of amorphous membrane. The COF membranes harvest proton conductivity up to 0.53 S cm -1 at 80 °C. Our strategy builds a bridge between amorphous polymeric and crystalline COF membranes, not only paving the way to large-scale fabrication of COF membranes, but also affording some guidance on materials processing via disorder-to-order transformation.
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http://dx.doi.org/10.1002/anie.202102965DOI Listing
June 2021

Electrocatalytic Methane Oxidation Greatly Promoted by Chlorine Intermediates.

Angew Chem Int Ed Engl 2021 Jun 1. Epub 2021 Jun 1.

Fudan University, Department of Chemistry, Fudan University, Shanghai, CHINA.

The renewable energy-powered methane (CH4) conversion at ambient conditions is an attractive but highly challenging field. Due to the highly inert feature of CH4, the selective cleavage of its first C-H bond without over-oxidation is essential for upgrading CH4 toward value-added products. In this work, we developed an efficient and selective CH4 conversion approach at room temperatures by intermediate chlorine species (*Cl), which were electrochemically generated and stabilized on cobalt-nickel mixed spinels with different Co/Ni ratios. The lower overpotentials for *Cl formation enabled effective activation and conversion of CH4 to CH3Cl without over-oxidation to CO2 , and Ni3+ at the octahedral sites in cobalt-nickel mixed spinels allowed to stabilize surface-bound *Cl species. The CoNi2Ox electrocatalyst exhibited an outstanding yield of CH3Cl (364 mmol·g-1·h-1 ) and a high CH3Cl-versus-CO2 selectivity of over 400 at room temperatures, with demonstrated capability of direct CH4 conversion under seawater working conditions.
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http://dx.doi.org/10.1002/anie.202105523DOI Listing
June 2021

Insight into electrocatalytic activity and mechanism of bimetal niobium-based oxides in situ embedded into biomass-derived porous carbon skeleton nanohybrids for photovoltaics and alkaline hydrogen evolution.

J Colloid Interface Sci 2021 May 12;601:12-29. Epub 2021 May 12.

Functional Materials Laboratory (FML), School of Materials Science and Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, China.

Developing highly-efficient multifunctional electrocatalysts for energy conversion devices is of great importance. A sequence of nano-sized bimetal (Al, Cr, Fe) niobium oxide nanoparticles anchored on aloe peel-derived porous carbon skeleton hybrids (AN/APPC, CN/APPC, and FN/APPC) are successfully prepared via co-precipitation avenue and used as electrocatalysts for photovoltaics and alkaline hydrogen evolution reaction. Benefiting from the synergies between nano-sized metal niobium oxides and highly conductive porous carbon skeleton, these robust polycomponent hybrid electrocatalysts exhibit superior catalytic performances for accelerating the triiodide reduction and hydrogen evolution reaction. The solar cell with AN/APPC electrocatalyst achieves an outstanding device efficiency of 7.31%, superior to that with Pt (6.84%), and the AN/APPC electrocatalyst exhibit an overpotential (131.6 mV) when the current density is 10 mA cm and Tafel slope (54 mV dec) in 1 M KOH for hydrogen evolution reaction. The AN/APPC electrocatalysts illustrate remarkable electrochemical durability in both I/I electrolyte and alkaline media. Furthermore, the catalytic mechanism was clarified both from the electronic structure and work function through first-principle density functional theory (DFT) calculations. This work opens a new avenue for electrocatalysis field via using nano-sized porous bio-carbon skeleton loaded with niobium-based binary metal.
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http://dx.doi.org/10.1016/j.jcis.2021.05.060DOI Listing
May 2021

Hydrogen alleviates acute lung injury induced by limb ischaemia/reperfusion in mice.

Life Sci 2021 May 28;279:119659. Epub 2021 May 28.

College of Basic Medical Sciences and Institute of Atherosclerosis, Shandong First Medical University & Shandong Academy of Medical Science, Taian 271000, China. Electronic address:

Aims: Limb ischaemia/reperfusion (LIR) occurs in various clinical conditions including critical limb ischaemia, abdominal aortic aneurysm, and traumatic arterial injury. Reperfusion of the acutely ischemic limb can lead to a systemic inflammation response and multiple organ dysfunction syndrome, further resulting in significant morbidity and mortality. Molecular hydrogen exhibits therapeutic activity for the treatment and prevention of many diseases. Our study investigated the possible therapeutic effects of hydrogen and its mechanism of action in a LIR-induced acute lung injury (ALI) model.

Materials And Methods: Limb ischaemia/-reperfusion model was established in mice. The hydrogen-saturated saline was administered by intraperitoneal injection. Protein level of nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase-1 (HO1) and nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 (NQO1) was evaluated by immunohistochemistry staining and western blotting. Autophagy-related molecules were evaluated by western blotting. Malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by assay kits. Quantification of ceramides in lung was performed by high-performance liquid chromatography-tandem mass spectrometry.

Key Findings: Molecular hydrogen exhibited a protective effect on the LIR-induced ALI model. Hydrogen decreased malondialdehyde and increased superoxide dismutase activity in lung tissues. Additionally, hydrogen activated Nrf2 signalling in lung tissues. Hydrogen could inhibit the upregulation of autophagy in the present rodent model. Furthermore, ceramide was accumulated in lung tissues because of LIR; however, hydrogen altered the accumulation status.

Significance: Molecular hydrogen was found to be therapeutically effective in the LIR-induced ALI model; the mechanisms of action included modulation of antioxidation and autophagy.
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http://dx.doi.org/10.1016/j.lfs.2021.119659DOI Listing
May 2021

An antibody-like peptidic network for anti-angiogenesis.

Biomaterials 2021 May 18;275:120900. Epub 2021 May 18.

CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing, 100190, China. Electronic address:

Different from chemical (small molecular inhibitor) and biological (monoclonal antibody) drugs, herein, based on angiogenesis-related neuropilin-1 (NRP-1), we develop a biomimetic superstructure drug, i.e. an antibody-like peptidic network (ALPN) to achieve the high-efficient treatment of choroidal neovascularization (CNV). The ALPN in nanoparticulated formulation (ALPN-NP) can bind NRP-1 through targeting unit and form fibrous peptidic networks trapping NRP-1 on the surface of endothelial cells (ECs), leading to anti-angiogenesis. The ALPN shows high-efficacy against angiogenesis in CNV rat model ascribed to the superstructure-enhanced binding and blockage of NRP-1. The very low dose of ALPN (0.263 μg/Kg) exhibits similar anti-angiogenesis effect comparing with monoclonal antibody bevacizumab (23.5 μg/Kg), which shows potential advantages over traditional monoclonal antibodies.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120900DOI Listing
May 2021

Promoting electrocatalytic carbon monoxide reduction to ethylene on copper-polypyrrole interface.

J Colloid Interface Sci 2021 May 18;600:847-853. Epub 2021 May 18.

Laboratory of Advanced Materials, Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Faculty of Chemistry and Materials Science, Fudan University, Shanghai 200438, China. Electronic address:

The renewable energy-powered electroreduction of carbon dioxide or monoxide (CO) has been emerging as an attractive means to decarbonize the emission-intensive chemical manufacturing, which heavily relies on fossil fuels nowadays. One potential approach to promote the activity of electrocatalysts is to construct hybrid interface that can increase the stability of intermediates on electrode surfaces. Herein we developed a copper nanoparticle/polypyrrole (Cu-Ppy) nanowire composite as an efficient electrocatalyst for electrochemical CO reduction reaction. Compared to pure Cu nanoparticles, the Cu-Ppy composite exhibited a dramatically enhanced Faradaic efficiency of converting CO to ethylene (CH) from 34% to 69% at -0.78 V vs. reversible hydrogen electrode (RHE) in KOH electrolyte, and an excellent CH partial current density of 276 mA·cm at -1.18 V vs. RHE. Density functional theory calculations showed that the Cu-Ppy composite could bind CO more strongly as compared to pure Cu. As the Ppy coating allowed to stabilize OCCO*, a key intermediate in the CH formation, both the activity and selectivity of Cu-Ppy for CO-to-CH were increased. Our work suggests that constructing rationally designed hybrid interface can tune the local environment of catalyst surface toward enhanced activity and product selectivity.
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http://dx.doi.org/10.1016/j.jcis.2021.05.057DOI Listing
May 2021

Impacts of solvation on photo-damage of polypeptides: Modulation and biological implications.

J Photochem Photobiol B 2021 Jul 20;220:112214. Epub 2021 May 20.

Department of Chemistry, College of Chemical Engineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, P R, China. Electronic address:

We investigate the photon/matter interactions between soft X-rays and three selected polypeptides, poly-glycine (poly-Gly), poly-L-arginine (poly-Arg), and poly-l-lysine (poly-Lys), where the effects of molecular packing under the influence of solvent, e.g., water, substrates (Au foil or Si wafer) and X-ray irradiation under different durations were systematically investigated. Compared with negligible photo-damage on bare polypeptide powders, significantly enhanced degradation in pre-solvated polypeptides was observed likely because of the formation photo-generated radicals. X-ray photoemission spectroscopy (XPS) were employed as the analysis means to identify and quantify the chemical changes, especially the high-resolution photoemission spectra of C 1s, O 1s, N 1s and their evolution under continuous X-ray irradiation. The photo-degradation was found to preferentially occur on the CO entity in poly-Gly and the guanidinium group in poly-Arg. In poly-Arg, deprotonation occurs via the switch from zwittterionic to a neutral configuration, whereas poly-Lys deprotonates by directly losing the corresponding amine. The critical role of the interactions between amino acids, the building blocks of protein and almost all forms of biological activities, and the free-radical-generating living environment under irradiation was critically analyzed. The present study found that the preparation history of a sample, especially its inadvertent exposure to the sources of HO, O and OH, could significantly alter the outcome of a radiation-related chemical process. Implications on the non-destructive probe of biologically important systems using physical methods involving X-rays were discussed as well.
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http://dx.doi.org/10.1016/j.jphotobiol.2021.112214DOI Listing
July 2021

Reduced expression of MiR-125a-5p aggravates LPS-induced experimental acute kidney injury pathology by targeting TRAF6.

Life Sci 2021 May 25:119657. Epub 2021 May 25.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address:

Aims: Patients with acute kidney injury (AKI) have higher mortality, and sepsis is among its main causes. MicroRNAs (miRNAs) are essential for regulating kidney function and could have curative potential. This study explored the possibility to treat AKI with miR-125a-5p and reveal the possible mechanism.

Materials And Methods: LPS-induced mouse model and LPS-induced RAW264.7 cell model of AKI were established and treated with miR-125a-5p mimics or inhibitors. Serum creatinine and blood urea were measured to evaluate kidney function. The pathological changes of kidney tissues were detected by H&E and PAS staining technique, and the infiltration of macrophages were observed by immunohistochemistry. RAW264.7 cell viability, TRAF6 and cytokines expressions under LPS stimulation were measured. The role and therapeutic potential of miR-125a-5p were verified in vivo and in vitro after given miR-125a-5p mimics or inhibitors.

Key Findings: LPS-induced mice had increasing serum creatinine and urea, and evident pathological changes, including severe tubular dilatation and macrophages infiltration. TRAF6 expression in the kidney was significantly higher, while miR-125a-5p expression was suppressed. MiR-125a-5p targeted TRAF6, and its overexpression deactivated NF-κB signaling pathway, reducing downstream TNF-α, IL-1β and IL-6 expressions. MiR-125a-5p mimics rescued LPS-induced kidney damage and suppressed pro-inflammatory cytokines expression through inhibiting TRAF6/NF-κB axis.

Significance: We highlighted that miR-125a-5p could inhibit LPS-induced acute inflammation in the kidney through targeting TRAF6/NF-κB axis. These results might contribute to the development of molecular therapy in AKI.
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http://dx.doi.org/10.1016/j.lfs.2021.119657DOI Listing
May 2021

The characteristics of spatial expansion and driving forces of land urbanization in counties in central China: A case study of Feixi county in Hefei city.

PLoS One 2021 26;16(5):e0252331. Epub 2021 May 26.

The Center for Modern Chinese City Studies, East China Normal University, Shanghai, China.

Urbanization in Central and Western China has attracted increasing attention in the advent of new-type urbanization in China and the age of 'Global Urbanism'. Although land urbanization is at the epicenter of Chinese urbanization, its process and driving forces in counties beyond the Eastern coastal areas are less known. This paper therefore seeks to investigate the spatial expansion and driving forces of land urbanization in Feixi county, a relatively advanced county neighboring Hefei city proper in Anhui province in Central China. Based on the land-use change survey data, remote sensing interpretation data, and statistical yearbook data of Feixi county from 2002 to 2016, it is revealed that the overall scale of urban land in Feixi county increased remarkably, though with obvious temporal and geographical variants. The year 2009 appears to be the cutting line between quantity-based land urbanization and quality-based land urbanization. Land urbanization rate is sensitive to both geographical distance and traffic accessibility to Hefei city proper. Driving forces of land urbanization in Feixi county are summarized as: (1) central city expansion, (2) government-led industrial park construction, and (3) large industrial projects along traffic corridors. A better understanding of urbanization in Feixi county cannot be fulfilled without taking into account the wider spatial process in Hefei city and the Yangtze River Delta city-region.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252331PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153434PMC
May 2021

The application of XGBoost and SHAP to examining the factors in freight truck-related crashes: An exploratory analysis.

Accid Anal Prev 2021 Aug 23;158:106153. Epub 2021 May 23.

Urban Mobility Institute, Key Laboratory of Road and Traffic Engineering, Ministry of Education at Tongji University, College of Transportation Engineering, Tongji University, China. Electronic address:

Due to the burgeoning demand for freight movement, freight related road safety threats have been growing substantially. In spite of some research on the factors influencing freight truck-related crashes in major cities, the literature offers limited evidence about the effects of the built environment on the occurrence of those crashes by injury severity. This article uses data from the Los Angeles region in 2010-2019 to explore the relationships between the built environment factors and the spatial distribution of freight truck-related crashes using XGBoost and SHAP methods. Results from the XGBoost model show that variables related to the built environment, in particular demographics, land uses and road network, are highly correlated to freight truck related crashes of all three injury types. The SHAP value plots further indicate the important nonlinear relationships between independent variables and dependent variables. This study also emphasizes the differences in modeling mechanisms between the XGBoost model and traditional statistical models. The findings will help transport planners develop operational measures for resolving the emerging freight truck related traffic safety problems in local communities.
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http://dx.doi.org/10.1016/j.aap.2021.106153DOI Listing
August 2021

Multicomponent Synthesis of α-Branched Tertiary and Secondary Amines by Photocatalytic Hydrogen Atom Transfer Strategy.

Org Lett 2021 Jun 24;23(11):4473-4477. Epub 2021 May 24.

State Key Lab of Urban Water Resource and Environment, School of Science, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.

A multicomponent carbonyl alkylative amination reaction is described. A variety of -arylamines, aldehydes, and hydrocarbons have been examined as reaction substrates using tetrabutylammonium decatungstate as photocatalyst, providing the corresponding α-branched tertiary and secondary amines in good to moderate yields. The reaction proceeds through the generation of alkyl radicals by a light-promoted hydrogen atom transfer process followed by free radical addition to iminium ions generated in situ.
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http://dx.doi.org/10.1021/acs.orglett.1c01412DOI Listing
June 2021

Atomically Dispersed Iron Metal Site in a Porphyrin-Based Metal-Organic Framework for Photocatalytic Nitrogen Fixation.

ACS Nano 2021 May 23. Epub 2021 May 23.

School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, People's Republic of China.

The rational design of photocatalysts for efficient nitrogen (N) fixation at ambient conditions is important for revolutionizing ammonia production and quite challenging because the great difficulty lies in the adsorption and activation of the inert N. Inspired by a biological molecule, chlorophyll, featuring a porphyrin structure as the photosensitizer and enzyme nitrogenase featuring an iron (Fe) atom as a favorable binding site for N π-backbonding, here we developed a porphyrin-based metal-organic framework (PMOF) with Fe as the active center as an artificial photocatalyst for N reduction reaction (NRR) under ambient conditions. The PMOF features aluminum (Al) as metal node imparting high stability and Fe incorporated and atomically dispersed by residing at each porphyrin ring promoting the adsorption and the activation of N, termed Al-PMOF(Fe). Compared with the pristine Al-PMOF, Al-PMOF(Fe) exhibits a substantial enhancement in NH yield (635 μg g) and production rate (127 μg h g) of 82% and 50%, respectively, on par with the best-performing MOF-based NRR catalysts. Three cycles of photocatalytic NRR experimental results corroborate a stable photocatalytic activity of Al-PMOF(Fe). The combined experimental and theoretical results reveal that the Fe-N site in Al-PMOF(Fe) is the active photocatalytic center that can mitigate the difficulty of the rate-determining step in photocatalytic NRR. The possible reaction pathways of NRR on Al-PMOF(Fe) were established. Our study of porphyrin-based MOF for the photocatalytic NRR will provide insight into the rational design of catalysts for artificial photosynthesis.
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http://dx.doi.org/10.1021/acsnano.0c10947DOI Listing
May 2021

Histone lysine methyltransferase SET8 is a novel therapeutic target for cancer treatment.

Drug Discov Today 2021 May 20. Epub 2021 May 20.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, 55 Daxuecheng South Road, Shapingba, Chongqing 401331, China. Electronic address:

SET8 is the only lysine methyltransferase that can specifically monomethylate the histone H4K20. SET8-mediated protein modifications are largely involved in the regulation of cell cycle, DNA repair, gene transcription, cell apoptosis, and other vital physiological processes. The aberrant expression of SET8 is closely linked to the proliferation, invasion, metastasis, and prognosis of a variety of cancers. As a consequence, targeting SET8 could be an appealing strategy for cancer therapy. In this article, we introduce the molecular structure of SET8, followed by summarizing its roles in various biological pathways. Crucially, we highlight the potential functions of SET8 in tumors, as well as progress in the development of SET inhibitors for cancer treatment.
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http://dx.doi.org/10.1016/j.drudis.2021.05.004DOI Listing
May 2021

Symmetric-Constrained Irregular Structure Inpainting for Brain MRI Registration with Tumor Pathology.

Brainlesion 2021 27;12658:80-91. Epub 2021 Mar 27.

Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Deformable registration of magnetic resonance images between patients with brain tumors and healthy subjects has been an important tool to specify tumor geometry through location alignment and facilitate pathological analysis. Since tumor region does not match with any ordinary brain tissue, it has been difficult to deformably register a patient's brain to a normal one. Many patient images are associated with irregularly distributed lesions, resulting in further distortion of normal tissue structures and complicating registration's similarity measure. In this work, we follow a multi-step context-aware image inpainting framework to generate synthetic tissue intensities in the tumor region. The coarse image-to-image translation is applied to make a rough inference of the missing parts. Then, a feature-level patch-match refinement module is applied to refine the details by modeling the semantic relevance between patch-wise features. A symmetry constraint reflecting a large degree of anatomical symmetry in the brain is further proposed to achieve better structure understanding. Deformable registration is applied between inpainted patient images and normal brains, and the resulting deformation field is eventually used to deform original patient data for the final alignment. The method was applied to the Multimodal Brain Tumor Segmentation (BraTS) 2018 challenge database and compared against three existing inpainting methods. The proposed method yielded results with increased peak signal-to-noise ratio, structural similarity index, inception score, and reduced L1 error, leading to successful patient-to-normal brain image registration.
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http://dx.doi.org/10.1007/978-3-030-72084-1_8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130838PMC
March 2021

Predicting Prolonged Length of Hospital Stay for Peritoneal Dialysis-Treated Patients Using Stacked Generalization: Model Development and Validation Study.

JMIR Med Inform 2021 May 19;9(5):e17886. Epub 2021 May 19.

National Institute of Health Data Science, Peking University, Beijing, China.

Background: The increasing number of patients treated with peritoneal dialysis (PD) and their consistently high rate of hospital admissions have placed a large burden on the health care system. Early clinical interventions and optimal management of patients at a high risk of prolonged length of stay (pLOS) may help improve the medical efficiency and prognosis of PD-treated patients. If timely clinical interventions are not provided, patients at a high risk of pLOS may face a poor prognosis and high medical expenses, which will also be a burden on hospitals. Therefore, physicians need an effective pLOS prediction model for PD-treated patients.

Objective: This study aimed to develop an optimal data-driven model for predicting the pLOS risk of PD-treated patients using basic admission data.

Methods: Patient data collected using the Hospital Quality Monitoring System (HQMS) in China were used to develop pLOS prediction models. A stacking model was constructed with support vector machine, random forest (RF), and K-nearest neighbor algorithms as its base models and traditional logistic regression (LR) as its meta-model. The meta-model used the outputs of all 3 base models as input and generated the output of the stacking model. Another LR-based pLOS prediction model was built as the benchmark model. The prediction performance of the stacking model was compared with that of its base models and the benchmark model. Five-fold cross-validation was employed to develop and validate the models. Performance measures included the Brier score, area under the receiver operating characteristic curve (AUROC), estimated calibration index (ECI), accuracy, sensitivity, specificity, and geometric mean (Gm). In addition, a calibration plot was employed to visually demonstrate the calibration power of each model.

Results: The final cohort extracted from the HQMS database consisted of 23,992 eligible PD-treated patients, among whom 30.3% had a pLOS (ie, longer than the average LOS, which was 16 days in our study). Among the models, the stacking model achieved the best calibration (ECI 8.691), balanced accuracy (Gm 0.690), accuracy (0.695), and specificity (0.701). Meanwhile, the stacking and RF models had the best overall performance (Brier score 0.174 for both) and discrimination (AUROC 0.757 for the stacking model and 0.756 for the RF model). Compared with the benchmark LR model, the stacking model was superior in all performance measures except sensitivity, but there was no significant difference in sensitivity between the 2 models. The 2-sided t tests revealed significant performance differences between the stacking and LR models in overall performance, discrimination, calibration, balanced accuracy, and accuracy.

Conclusions: This study is the first to develop data-driven pLOS prediction models for PD-treated patients using basic admission data from a national database. The results indicate the feasibility of utilizing a stacking-based pLOS prediction model for PD-treated patients. The pLOS prediction tools developed in this study have the potential to assist clinicians in identifying patients at a high risk of pLOS and to allocate resources optimally for PD-treated patients.
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http://dx.doi.org/10.2196/17886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173398PMC
May 2021

Global myocardial work: A new way to detect subclinical myocardial dysfunction with normal left ventricle ejection fraction in essential hypertension patients: Compared with myocardial layer-specific strain analysis.

Echocardiography 2021 Jun 18;38(6):850-860. Epub 2021 May 18.

Department of Interventional Radiology, the First Affiliated Hospital of Soochow University, Suzhou, China.

Objectives: The aim of this study was to determine whether global myocardial work (MW), derived from non-invasive left ventricle (LV) pressure-strain loops (PSL) at rest, could predict subclinical LV myocardial dysfunction in preserved left ventricular ejection fraction (LVEF) essential hypertension (EHT) patients.

Methods: A total of 105 untreated EHT patients and 55 normal controls were enrolled in this study. Apical 4-, 3- and 2-chamber views were acquired by two-dimensional echocardiography. The peak systolic myocardial layer-specific longitudinal strain (epimyocardial: GLSEpi; middle layer: GLSMid; and endomyocardial: GLSEndo), global myocardial work index (GWI), global constructive work (GCW), global wasted work (GWW), and myocardial work efficiency (GWE) were generated by speckle-tracking echocardiography (STE).

Results: The values of GLSEpi, GLSMid, and GLSEndo were significantly lower in EHT patients with LVH than in EHT patients without LVH and normal controls. GWW was significantly increased in EHT patients with LVH compared with without LVH and normal subjects, while GWE was significantly reduced in EHT patients with LVH compared with without LVH and normal subjects. ROC analysis showed that combined global MW values were a more sensitive predictor for detecting the accuracy of LV subclinical dysfunction in EHT patients than layer-specific GLS.

Conclusion: From the research, we conclude that global MW is more sensitive to layer-specific GLS in its ability to detect subclinical LV dysfunction even in EHT patients even without LVH.
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http://dx.doi.org/10.1111/echo.15063DOI Listing
June 2021