Publications by authors named "Chao Xue"

150 Publications

Clinicopathologic and genetic features of metaplastic breast cancer with osseous differentiation: a series of 6 cases.

Breast Cancer 2021 May 3. Epub 2021 May 3.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Aims: Metaplastic breast cancer (MBC) comprises a heterogeneous group of tumors, of which MBC with osseous differentiation (MBC-OD) is extremely rare that only few cases have been reported. This study aimed to present the clinicopathologic and molecular features of MBC-OD.

Methods: We collected 6 cases of MBC-OD from five different centers and described its clinicopathologic and molecular characteristics based on the next-generation sequencing. Another 11 cases from the literature were also reviewed to better characterize the tumor.

Results: The tumor primarily showed an osteosarcoma-like appearance, which composed of high cellularity with spindle cells and osteoblast-like cells producing coarse lace-like neoplastic bone (4/6) or osteoid matrix (6/6). 55 somatic mutations including 39 missenses (70.9%), 9 frameshifts (16.4%), 3 splice sites (5.5%), 3 in-frame InDels (5.5%) and 1 nonsense (1.8%) were identified. TP53 was the most frequently mutated genes (5/6, 83.3%), followed by RB1 (3/6, 50.0%), BCOR (2/6, 33.3%), MED12 (2/6, 33.3%), PIK3CA (2/6, 33.3%), and TET2 (2/6, 33.3%). Genetic alterations suggesting therapies with clinical benefit, including mTOR inhibitors, tyrosine kinase inhibitors (TKI), and poly-ADP ribose polymerase inhibitor (PARPi), were observed in five cases. The median follow-up was 21 months (range, 3-80 months). Local recurrence was observed in two cases and three patients displayed distant metastasis. Two patients died of the disease at 3 months and 7 months, respectively.

Conclusions: Based on this series, MBC-OD is a highly aggressive breast tumor with osteosarcoma-like morphology and a high incidence of recurrent disease showing specific genetic profiles.
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http://dx.doi.org/10.1007/s12282-021-01246-9DOI Listing
May 2021

Clinical profiles and outcomes of acute type A aortic dissection and intramural hematoma in the current era: lessons from the first registry of aortic dissection in China.

Chin Med J (Engl) 2021 Apr 13;134(8):927-934. Epub 2021 Apr 13.

Department of Cardiovascular Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Background: Acute type A aortic dissection (ATAAD) and acute type A intramural hematoma (ATAIMH) are life-threatening diseases with high mortality. To better understand their clinical features in the Chinese population, we analyzed the data from the first Registry of Aortic Dissection in China (Sino-RAD) to promote the understanding and management of the diseases.

Methods: All patients with ATAAD and ATAIMH enrolled in Sino-RAD from January 1, 2012 to December 31, 2016 were involved. The data of patients' selection, history, symptoms, management, outcomes, and postoperation complications were analyzed in the study. The continuous variables were compared using the Student's t test for normal distributions and the Mann-Whitney U test for non-normal distributions. Categorical variables were compared using the Chi-square test or Fisher exact test.

Results: A total of 1582 patients with ATAAD and 130 patients with ATAIMH were included. The mean age of all patients was 48.4 years. Patients with ATAAD were significantly younger than patients with ATAIMH (48.9 years vs. 55.6 years, P < 0.001). For the total cohort, males were dominant, but the male ratio of patients with ATAAD was significantly higher compared to those with ATAIMH (P = 0.01). The time range from the onset of symptom to hospitalization was 2.0 days. More patients of ATAIMH had hypertension than that of ATAAD (82.3% vs. 67.6%, P < 0.05). Chest and back pain were the most common clinical symptoms. Computerized tomography (CT) was the most common initial diagnostic imaging modality. 84.7% received surgical treatment and in-hospital mortality was 5.3%. Patients with ATAAD mainly received surgical treatment (89.6%), while most patients with ATAIMH received medical treatment (39.2%) or endovascular repair (35.4%).

Conclusions: Our study suggests that doctors should comprehensively use clinical examination and genetic background screening for patients with ATAAD and ATAIMH and further shorten the time range from symptoms onset to intervention, achieving early diagnosis and treatment, thereby reducing the mortality rate of patients with aortic dissection in China. We should standardize the procedures of aortic dissection treatment and improve people's understanding. Meanwhile, the curing and transferring efficiency should also be improved.
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http://dx.doi.org/10.1097/CM9.0000000000001459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078379PMC
April 2021

FUS/TLS suppresses enterovirus replication and promotes antiviral innate immune responses.

J Virol 2021 Apr 7. Epub 2021 Apr 7.

Centre for Heart and Lung Innovation, St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada

During viral infection, the dynamic virus-host relationship is constantly in play. Many cellular proteins such as RNA-binding proteins (RBPs) have been shown to mediate antiviral responses during viral infection. Here we reported that the RBP, fused in sarcoma/translocated in liposarcoma (FUS/TLS), acts as a host restricting factor against the infection of coxsackievirus B3 (CVB3). Mechanistically, we found that deletion of FUS leads to increased viral RNA transcription and enhanced internal ribosome entry site (IRES)-driven translation, with no apparent impact on viral RNA stability. We further demonstrated that FUS physically interacts with viral genome, which may contribute to direct inhibition of viral RNA transcription/translation. Moreover, we identified a novel function for FUS in regulating host innate immune response. We showed that in the absence of FUS, gene expression of type I interferons and proinflammatory cytokines elicited by viral or bacterial infection is significantly impaired. Emerging evidence suggests a role for stress granules (SGs) in antiviral innate immunity. We further uncovered that knockout of FUS abolishes the ability to form SGs upon CVB3 infection or polyinosinic:polycytidylic acid (polyIC) treatment. Finally, we showed that, to avoid FUS-mediated antiviral response and innate immunity, CVB3 infection results in cytoplasmic mislocalization and cleavage of FUS through the enzymatic activity of viral proteases. Together, our findings in this study identified FUS as a novel host antiviral factor, which restricts CVB3 replication through direct inhibition of viral RNA transcription and protein translation and by regulating host antiviral innate immunity.Enteroviruses are common human pathogens, including those that cause myocarditis (coxsackievirus B3, CVB3), poliomyelitis (poliovirus) and hand, foot and mouse disease (enterovirus 71). Understanding the virus-host interaction is crucial for finding the treatment and prevention of these pathogens. In this study, we explored the interplay between host RNA binding protein FUS/TLS and CVB3 and reported that FUS/TLS restricts CVB3 replication through direct inhibition of viral RNA transcription/translation and by regulation of cellular antiviral innate immunity. To impede the antiviral role of FUS, CVB3 targets FUS for mislocalization and cleavage. Findings from this study provide novel insights into interactions between CVB3 and the FUS, which may lead to novel therapeutic interventions against enterovirus-induced diseases.
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http://dx.doi.org/10.1128/JVI.00304-21DOI Listing
April 2021

Higenamine attenuates cardiac fibroblast abstract and fibrosis via inhibition of TGF-β1/Smad signaling.

Eur J Pharmacol 2021 Jun 23;900:174013. Epub 2021 Mar 23.

Department of Cardiology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 274 Zhijiang RD, Shanghai, 200071, China; Aab Cardiovascular Research Institute, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Ave Box CVRI, Rochester, NY, 14642, United States. Electronic address:

Rationale: Higenamine (HG), is one of the main active components in many widely used Chinese herbs, and a common ingredient of health products in Europe and North America. Several groups, including our own, have previously shown the beneficial effects of HG against cardiomyocyte death during acute ischemic damage. However, the effect of HG on chronic cardiac remodeling, such as cardiac fibrosis, remains unknown.

Objective: Herein, we aim to investigate the role of HG in cardiac fibrosis in vivo as well as its cellular and molecular mechanisms.

Methods And Results: Chronic pressure overload with transverse aortic constriction (TAC) significantly increased cardiac hypertrophy, fibrosis, and cardiac dysfunction in mice, which were significantly attenuated by HG. Consistently, cardiac fibrosis induced by the chronic infusion of isoproterenol (ISO), was also significantly reduced by HG. Interestingly, our results showed that HG had no effect on adult mouse CM hypertrophy in vitro. However, HG suppressed the activation of cardiac fibroblasts (CFs) in vitro. Furthermore, TGF-β1-induced expression of ACTA2, a marker of fibroblast activation, was significantly suppressed by HG. Concomitantly, HG inhibited TGF-β1-induced phosphorylation of Smad2/3 in CFs. HG also reduced the expression of extracellular matrix molecules such as collagen I and collagen III. To our surprise, the inhibitory effect of HG on CFs activation was independent of the activation of the beta2 adrenergic receptor (β2-AR) that is known to mediate the effect of HG on antagonizing CMs apoptosis.

Conclusion: Our findings suggest that HG ameliorates pathological cardiac fibrosis and dysfunction at least partially by suppressing TGF-β1/Smad signaling and CFs activation.
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http://dx.doi.org/10.1016/j.ejphar.2021.174013DOI Listing
June 2021

The therapeutic effect of plasma exchange on ANCA-associated vasculitis: A meta-analysis.

Clin Nephrol 2021 Mar 22. Epub 2021 Mar 22.

Aims: The therapeutic effect of plasma exchange (PLEX) combined with conventional treatment in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) remains controversial.

Materials And Methods: We searched PubMed, Embase, Cochrane Library, and China National Knowledge Infrastructure for randomized controlled trials (RCTs) and cohort studies that compared PLEX added to conventional therapy with conventional therapy only in active AAV.

Results: 19 studies were included for the meta-analysis. Compared with the conventional therapy group, the PLEX group had a significantly reduced risk of end-stage renal disease (ESRD) at 3 months (odds ratio (OR) = 0.32, 95% confidence interval (CI) = 0.16 - 0.66, p = 0.002, I2 = 0%), and the ANCA titerwas also decreased (OR = 40.99, 95% Cl = 23.56 - 58.43, p < 0.00001, I2 = 42%). The plasma and non-plasma exchange groups had no substantial differences in terms of short- and long-term outcomes, including all-cause mortality, ESRD risk at 12 months and 5 years, remission rate, serum creatine levels, or serious adverse events.

Conclusion: PLEX therapy was not associated with favorable long-term outcomes, although the results showed benefits in the incidence of ESRD rate at 3 months and ANCA titers in patients with AAV. No advantage of PLEX added to conventional therapy on mortality and complete remission was observed in patients with diffuse alveolar hemorrhage. Further high-quality multicenter RCTs with a high number of participants are required to assess the potential efficacy of PLEX in active AAV.
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http://dx.doi.org/10.5414/CN110410DOI Listing
March 2021

Prediction Potency of Gonadal Hormone Alterations on Sexual Dysfunction After Hematopoietic Stem Cell Transplantation.

Reprod Sci 2021 Mar 15. Epub 2021 Mar 15.

Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250021, China.

Sexual dysfunction (SD) is one of the late complications in survivors after hematopoietic stem cell transplantation (HSCT), and the gonadal hormones might be involved in the pathogenesis of this pathological process. This study aimed to investigate the incidence of SD by questionnaire, to explore the relationship between SD and the comprehensive gonadal hormones in patients post HSCT. We identified 72 survivors of hematological diseases who underwent HSCT. The sociodemographic characteristics and medical histories of participants were ascertained by a modified version of a questionnaire named "PPSAS-HSCT" in our study. Blood samples were regularly assayed for the global gonadal hormones. Forty-four percent of the females and 51% of the males reported a loss of interest in sexual activities. Ninety-two percent (23/25) of females exhibited decreased serum anti-Müllerian hormone (AMH) levels, and 74% (35/47) of males had elevated follicle-stimulating hormone (FSH) levels. The males with a higher level of oestradiol/testosterone (E2/T) had more symptoms of SD after HSCT. Patients with GVHD who received glucocorticoid (GC) therapy exhibited a lower level of testosterone and more serious SD, especially in the female population. SD and abnormal gonadal hormone homeostasis were present in more than half of the survivors after HSCT. Graft-versus-host disease (GVHD) and glucocorticoid treatment were confirmed to have a significant impact on the levels of testosterone among females. A multimodal intervention for the survivors after HSCT and a better consciousness of the medical staff are necessary for improving the quality of life of the recipients.
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http://dx.doi.org/10.1007/s43032-021-00536-zDOI Listing
March 2021

Effect of L-carnitine supplementation on renal anemia in patients on hemodialysis: a meta-analysis.

Int Urol Nephrol 2021 Mar 13. Epub 2021 Mar 13.

Department of Nephrology, The First Affiliated Hospital of University of South China, Hengyang, China.

Background: L-carnitine is an amino acid derivative that is thought to be helpful for treating renal anemia in hemodialysis patients. However, the mechanism remains to be fully elucidated.

Methods: A literature search was performed on PubMed, Embase, and Cochrane Central Register of Controlled Trials to identify randomized controlled trials (RCTs) and conduct a meta-analysis for investigating the effect of L-carnitine in the treatment of renal anemia in participants receiving hemodialysis.

Results: A total of 18 eligible trials with 1090 participants were included in this study. L-carnitine can significantly increase plasma free L-carnitine levels (mean difference [MD]: 140.53, 95% confidence interval [CI] 102.22-178.85; P < 0.00001), decrease the erythropoietin responsiveness index (ERI; MD: -2.72, 95% CI -3.20 to -2.24; P < 0.00001) and the required erythropoiesis-stimulating agent (ESA) doses (MD: -1.70, 95% CI -2.04 to -1.36; P < 0.00001). However, the use of L-carnitine was not associated with a higher hemoglobin level (MD: 0.18, 95% CI -0.20 to 0.55; P = 0.35) and hematocrit level (MD: 1.07, 95% CI -0.73 to 2.87; P = 0.24). In subgroup analyses, the effects of L-carnitine supplementation on renal anemia in patients on hemodialysis were independent of the treatment duration and intervention routes.

Conclusion: The present meta-analysis indicated that L-carnitine therapy significantly increased plasma L-carnitine concentrations, improved the response to ESA, decreased the required ESA doses in patients receiving hemodialysis, and maintained hemoglobin and hematocrit levels. L-carnitine supplementation should be supported in hemodialysis patients. However, the relationship between L-carnitine treatment and long-term outcomes is still unclear. Further high-quality RCTs are needed to verify our findings.
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http://dx.doi.org/10.1007/s11255-021-02835-5DOI Listing
March 2021

Exploring the Molecular Mechanism of Blue Flower Color Formation in cv. "Forever Summer".

Front Plant Sci 2021 17;12:585665. Epub 2021 Feb 17.

College of Landscape and Horticulture, Hunan Agricultural University, Changsha, China.

has a large inflorescence and rich colors, which has made it one of the most popular ornamental flowers worldwide. Thus far, the molecular mechanism of flower color formation in flowers is unknown. By comparing the pigment content and transcriptome data of the bud period (FSF1), discoloration period (FSF2) and full-bloom stage (FSF3) of infertile blue flowers of cv. "Forever Summer," we found that genes associated with anthocyanin production were most associated with the formation of blue infertile flowers throughout development. The anthocyanin biosynthesis pathway is the main metabolic pathway associated with flower color formation, and the carotenoid biosynthesis pathway appeared to have almost no contribution to flower color. There was no competition between the flavonoid and flavonol and anthocyanin biosynthesis pathways for their substrate. At FSF1, the key genes and in the flavonoid biosynthesis pathway were up-regulated, underlying the accumulation of a substrate for anthocyanin synthesis. By FSF3, the downstream genes , , , , and in the anthocyanin biosynthesis pathway were almost all up-regulated, likely promoting the synthesis and accumulation of anthocyanins and inducing the color change of infertile flowers. By analyzing protein-protein interaction networks and co-expression of transcription factors as well as differentially expressed structural genes related to anthocyanin synthesis, we identified negatively regulated transcription factors such as WER-like, MYB114, and WDR68. Their site of action may be the key gene in the anthocyanin biosynthesis pathway. The potential regulatory mechanism of flower color formation may be that WER-like, MYB114, and WDR68 inhibit or promote the synthesis of anthocyanins by negatively regulating the expression of . These results provide an important basis for studying the infertile flower color formation mechanism in and the development of new cultivars with other colors.
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http://dx.doi.org/10.3389/fpls.2021.585665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925886PMC
February 2021

Using iron-based phosphate binders in phosphate reduction and anemia improvement in patients receiving dialysis: a meta-analysis of randomized controlled trials.

Int Urol Nephrol 2021 Mar 6. Epub 2021 Mar 6.

Department of Nephrology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.

Purpose: A study was conducted to determine whether iron-based phosphate binders (IBPBs) need to be preferred for hyperphosphatemia and anemia management in patients on dialysis.

Methods: For this meta-analysis, we searched PubMed, Embase, and Cochrane Central Register of Controlled Trials for randomized controlled trials that evaluated the efficacy and safety of IBPBs in decreasing phosphate and correcting anemia in dialysis patients.

Results: Nineteen trials comprising 4719 participants were included. Compared with placebo, serum phosphate decreased significantly after treatment with ferric citrate (FC), fermagate (one study), and SBR759 (one study). Hemoglobin increased significantly after treatment with FC and sucroferric oxyhydroxide (PA21). In addition, FC and PA21 reduced serum intact parathyroid hormone (iPTH) and increased ferritin and transferrin saturation, but SBR759 did not. Compared with active treatment, the non-inferiority of IBPBs in reducing serum phosphate and iPTH was demonstrated. FC significantly improved serum hemoglobin and iron-related parameters and decreased the use of intravenous iron and erythropoiesis-stimulating agent, whereas PA21 did not increase serum hemoglobin level. The incidences of infection and hospitalization were similar between the two groups, with FC having a higher risk of diarrhea than the placebo and active treatments.

Conclusion: FC was associated with the control of hyperphosphatemia and the improvement of anemia. However, PA21 did not show superiority for alleviating anemia compared with the active treatment. Other IBPBs, such as fermagate and SBR759, remained poorly understood due to the limited number of studies. Further trials are required to assess the effect of IBPBs on the risk of cardiovascular events and all-cause mortality.
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http://dx.doi.org/10.1007/s11255-021-02820-yDOI Listing
March 2021

Potential effects of Rhodococcus qingshengii strain djl-6 on the bioremediation of carbendazim-contaminated soil and the assembly of its microbiome.

J Hazard Mater 2021 Feb 24;414:125496. Epub 2021 Feb 24.

Department of Microbiology, Key Lab of Environmental Microbiology for Agriculture, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

As a widely used fungicide, the environmental fate of carbendazim and its residues in agricultural products have caused great concern. However, its effects on soil microbial communities are largely unknown. Herein, we used high-throughput sequencing to reveal the effects of high and low dose of carbendazim and its degrading strain, Rhodococcus qingshengii strain djl-6, on the composition, diversity, and interrelationship of soil bacterial and fungal communities in short- and medium-term under laboratory conditions. The results showed that carbendazim exhibited an increased negative impact on bacterial communities and reduced the proportion of dominant fungal phylum Ascomycota during a 14-day incubation period. Only the impacts of low-dose carbendazim (2 mg·kg dry soil) on fungal community were weakened. Network analysis showed that carbendazim increased the connectivity and modularity of microbial co-occurrence networks. Strain djl-6 exhibited good potential for bioremediation of carbendazim-contaminated soils. Moreover, it driven the assembly of potential carbendazim-degrading consortia from indigenous microbial communities; and members of the genera Arthrobacter, Bacillus, Brevundimonas, Lysinibacillus, Massilia, Mycobacterium, Paenibacillus, and Pseudarthrobacter might be participated in the degradation of carbendazim. Taken together, our study provides a relatively comprehensive understanding of the effects of carbendazim and its degrading strain djl-6 on soil microbial communities.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125496DOI Listing
February 2021

Wilms tumor gent 1 (WT1)-specific adoptive immunotherapy in hematologic diseases.

Int Immunopharmacol 2021 May 28;94:107504. Epub 2021 Feb 28.

Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, China; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China; School of Medicine, Shandong University, Jinan, Shandong 250012, China. Electronic address:

As an attractive tumor-associated antigen (TAA), Wilms tumor gene 1 (WT1) is usually overexpressed in malignant hematological diseases. In recent years, WT1-specific adoptive immunotherapy has been the "hot spot" for tumor treatment. The main immunotherapeutic techniques associated with WT1 include WT1-specific cytotoxic T lymphocytes (CTLs), vaccine, and T cell receptor (TCR) gene therapy. WT1-based adoptive immunotherapy exhibited promising anti-tumorous effect with tolerable safety. There are still many limitations needed to be improved including the weak immunogenetics of WT1, immune tolerance, and short persistence of the immune response. In this review, we summarized the progress of productive technologies and the clinical or preclinical investigations of WT1-specific immunotherapy in hematological diseases.
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http://dx.doi.org/10.1016/j.intimp.2021.107504DOI Listing
May 2021

Nicotine exacerbates atherosclerosis through a macrophage-mediated endothelial injury pathway.

Aging (Albany NY) 2021 02 24;13(5):7627-7643. Epub 2021 Feb 24.

Department of Cardiology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.

Evidence suggests that nicotine intake promotes atherosclerosis. We enrolled 100 patients with coronary heart disease (CHD) and found that plaque burden, TXNIP expression, and inflammatory chemokine levels were higher in smokers than non-smokers. Additionally, patients with higher TXNIP expression in peripheral blood mononuclear cells (PBMCs) had a higher Gensini Scores and higher plasma IL-1β and IL-18 levels. Treating bone marrow-derived macrophages (BMDMs) with nicotine led to enhanced lipid phagocytosis, chemotaxis, and increased production of reactive oxygen species (ROS), which activated TXNIP/NLRP3 inflammasome signaling and promoted pyroptosis, as evidenced by caspase-1 cleavage and increased production of IL-1β, IL-18, and gasdermin D. Nicotine intake by ApoE mice fed a high-fat diet recapitulated those phenotypes. The effects of nicotine on pyroptotic signaling were reversed by N-acetyl-cysteine, a ROS scavenger. Silencing TXNIP reversed the effects of nicotine on macrophage invasion and vascular injury. Nicotine also induced pyroptotic macrophages that contributed to the apoptotic death of endothelial cells. These findings suggest that nicotine accelerates atherosclerosis in part by promoting macrophage pyroptosis and endothelial damage. Therefore, targeting the TXNIP/NLRP3-mediated pyroptotic pathway in macrophages may ameliorate nicotine-induced endothelial damage.
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http://dx.doi.org/10.18632/aging.202660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993665PMC
February 2021

Systematic Review of Interventions to Increase Awareness of Ultraviolet Radiation-Induced Harm and Protective Behaviors in Post-Secondary School Adults.

J Cutan Med Surg 2021 Feb 10:1203475420988863. Epub 2021 Feb 10.

266254257 Division of Dermatology, Department of Medicine, University of Toronto and Toronto Western Hospital, ON, Canada.

College and university students are a group known for excessive sun exposure and indoor tanning. Health education campaigns for avoidance of ultraviolet (UV) radiation have been relatively unsuccessful in this population. This systematic review examines interventions aimed at post-secondary school young adults on college and university campuses for skin cancer awareness, photoprotection, and change in UV-exposure-related behavior. Fifty-nine studies were identified for inclusion according to predetermined criteria. Study heterogeneity was high; methods of intervention were individual or group-based, and were mostly visually delivered and/or passive learning. Most interventions occurred at a single time point. Intervention success was assessed by evaluating subject behavior, intention, attitudes, knowledge, and emotion. Multicomponent interventions, generally consisting of UV photography and a passively delivered educational component, may be more effective than a single component alone. Overall, study quality was poor. Sample size of the majority of studies was <150 subjects. Most studies used self-report of behavior and had a short follow-up time. Generalizability of findings may be impacted as women, particularly white/Caucasian women, were overrepresented in the studies identified by this systematic review. For this specific target population, themes arising from the review include the importance of self-relevance and message framing. Self-affirmation was identified as a potential challenge in designing interventions for this target group, which can lead to defensiveness and a negative reaction to the health message. The findings of this systematic review may inform future research in this field, as well as guide planning of effective interventions in this target population.
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http://dx.doi.org/10.1177/1203475420988863DOI Listing
February 2021

An association between cumulative exposure to light at night and the prevalence of hyperuricemia in steel workers.

Int J Occup Med Environ Health 2021 Feb 4. Epub 2021 Feb 4.

North China University of Science and Technology, Tangshan, China (School of Public Health).

Objectives: Exposure to light at night (LAN) can disturb circadian endocrine and metabolic rhythms. Hyperuricemia (HUA) is an early-onset metabolic disorder. However, it is still not clear whether LAN exposure increases the prevalence of HUA.

Material And Methods: The authors used crosssectional data on the Beijing-Tianjin-Hebei occupational populations cohort from March-July 2017. A total of 7664 steel workers were finally selected to investigate the relationship between LAN exposure and the prevalence of HUA among steel workers. The authors collected demographic and socio-economic data, as well as information on lifestyle factors, anthropometric measures, and laboratory tests. The restricted cubic spline method was used to analyze the dose-response relationship between cumulative LAN exposure and the prevalence of HUA. Logistic regression analyses were used to fit the relationship between them.

Results: The average age of the participants was 43.5±8.6 years; 7051 (91.7%) of them were males, 2749 (35.9%) reported to suffer from HUA, and 1241 (16.2%) were not exposed to LAN. There was a significant non-linear dose-response relationship between them. After adjustment for the confounding factors, including demographic data, lifestyle factors, etc., the lower LAN exposure was significantly associated with HUA (0-1931.7 days, OR = 1.180, and the 95% CI: 1.000-1.394; 1931.7-4343 days, OR: 1.215, 95% CI: 1.035-1.426).

Conclusions: This study revealed that a certain amount of exposure to LAN is independently related to the prevalence of HUA in steel workers in China.
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http://dx.doi.org/10.13075/ijomeh.1896.01648DOI Listing
February 2021

Function Analysis of P450 and GST Genes to Imidacloprid in (Koch).

Front Physiol 2020 20;11:624287. Epub 2021 Jan 20.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

(Koch) is an economically important pest that affects legumes in worldwide. Chemical control is still the primary efficient method for management. However, the mechanism underlying insecticide resistance in has not been elucidated. A previous study observed that piperonyl butoxide (PBO) and diethyl maleate (DEM) significantly synergized imidacloprid in field populations, indicating that cytochrome P450 (P450) and glutathione S-transferase (GST) genes may play pivotal roles in imidacloprid resistance. In this study, 38 P450 genes and 10 GST genes were identified in through transcriptomic analysis. The expression levels of these P450 and GST genes were measured in susceptible (SUS) strains of under imidacloprid treatment with LC, LC, and LC doses. The expression levels of , , , , , , and were up-regulated in the three treatments. Most of these genes belong to CYP3 and CYP4 Clans. In addition, the expression levels of all P450 and GST genes in were also measured in the Juye (JY) and Linqing (LQ) field populations. The expression levels of , , and were up-regulated in the SUS strain after imidacloprid treatment at three doses, and these genes were overexpressed in the JY population. Furthermore, the sensitivity of to imidacloprid was significantly increased after knockdown of and through RNA interference. These results may help to elucidate the mechanisms underlying of imidacloprid resistance in .
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http://dx.doi.org/10.3389/fphys.2020.624287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854575PMC
January 2021

Application of high-throughput gene sequencing in lymphoma.

Exp Mol Pathol 2021 04 23;119:104606. Epub 2021 Jan 23.

Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, China. Electronic address:

As a malignant tumor originating from the lymphoid hematopoietic tissues, lymphoma has an increased incidence in recent years and has ranked among the top ten malignant tumors in the world. But until now, due to the multiple pathological subtypes and the unclear molecular mechanism, it's still difficult to make rapid diagnosis and accurate prognosis assessment for lymphoma patients. Recently, the development of high-throughput gene sequencing technology has provided the possibility to solve these clinical problems. This technology has realized large-scale screening of specific markers for lymphoma at the molecular biology level, followed by discovery of prognostic indicators and biological targets for new drug research. In this paper, we summarize the results of large-scale high-throughput gene sequencing research, and introduce the genetic changes associated with occurrence and prognosis of lymphomas with different pathological subtypes, hoping to further promote the application of this technology in clinical research of lymphoma.
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http://dx.doi.org/10.1016/j.yexmp.2021.104606DOI Listing
April 2021

Glutaraldehyde and 2,3-butanediol treatment of bovine pericardium for aortic valve bioprosthesis in sheep: a preliminary study.

Ann Transl Med 2020 Dec;8(24):1668

Department of Cardiovascular Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, China.

Background: Bovine pericardium can be used for cardiovascular repair surgeries, but challenges involving biocompatibility and durability remain. This study aimed to carry out pre-clinical testing of aortic valve replacement using an aortic valve prosthesis made of bovine pericardium modified with glutaraldehyde (GA) and 2,3-butanediol (BD).

Methods: The mechanical, plasma protein adsorption, platelet adhesion, collagenase digestion, and ninhydrin properties of the material (control GA GA + BD) were tested. All 3 tissues were implanted in rats and observed after 8 weeks under microscopy with alizarin red staining for calcification. Aortic valves made from the fully-treated material were implanted in sheep. A commercial bioprosthesis was used as control. Effectiveness and safety indicators were observed at 180 days after implantation.

Results: Compared with the control group, the GA + BD material showed higher elongation at breaking and tensile load (both P<0.05), lower plasma protein adsorption, lower platelet adhesion, lower collagenase digestion, lower ninhydrin value, and higher cross-linking (all P<0.05). After implantation in rat models, the GA + BD material showed little or no dissolution; there was no obvious calcification; and it was surrounded by a small amount of fibrosis, with peripheral capillary proliferation. After implantation in sheep models, the aortic valve leaflets of the experimental animals freely opened and closed, their surface was smooth, and no abnormal echo was observed. The echocardiographic results and hemodynamic were comparable between the two groups. All safety parameters were normal.

Conclusions: Modification of bovine pericardium with GA and BD results in a biomaterial with favorable properties for use as an aortic valve prosthesis.
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http://dx.doi.org/10.21037/atm-20-7803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812161PMC
December 2020

Aluminum-based metal-organic frameworks (CAU-1) highly efficient UO and TcO ions immobilization from aqueous solution.

J Hazard Mater 2021 Apr 8;407:124729. Epub 2020 Dec 8.

School of Life Science, Shaoxing University, Huancheng West Road 508, Shaoxing 312000, PR China. Electronic address:

In this research, an Al-based metal-organic framework (MOFs), CAU-1 was prepared through complexation between 2-aminoterephthalic acid and Al (III) by solvothermal approach, and simple operation and cost-effective synthetic route. The objective was to immobilize the typical positive/negative radionuclide ions (UO/TcO) in aqueous solution. The synthesized CAU-1 was characterized by XRD, FT-IR, TGA, FESEM, TEM-SAED, pH, XPS and N physisorption analysis. The structure of CAU-1 possessed excellent thermostability, rich functional groups (‒NH and ‒OH groups), as well as large surface area (1636.3 m/g) and the micropore volume (0.51 m/g). Furthermore, batch experiments demonstrated that CAU-1 with superior adsorption capacity was 648.37 (UO) mg/g and 692.33 (ReO) mg/g calculating from Langmuir isotherm model, respectively. Thermodynamic investigation showed the adsorption process was endothermic and spontaneous. In addition, the adsorption mechanism of ReO ion onto CAU-1 could be electrostatic attraction and chelation effect, while for UO ion, was mainly chelation effect induced by nitrogen-containing and oxygen-containing functional groups. Hence, the inexpensive and high-capacity CAU-1 could be considered as a practical material for sequestrations of radioactive pollutants from water environment.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124729DOI Listing
April 2021

Different exposure metrics of rotating night shift work and hyperhomocysteinaemia among Chinese steelworkers: a cross-sectional study.

BMJ Open 2020 12 15;10(12):e041576. Epub 2020 Dec 15.

Department of Epidemiology and Health Statistics, School of Public Health, North China University of Science and Technology, Tangshan, Hebei Province, China

Objective: To examine the associations of rotating night shift work with hyperhomocysteinaemia (HHcy) odds by different exposure metrics.

Design: Cross-sectional study.

Setting: Occupational physical examination centre for steel production workers, Tangshan, China.

Participants: A total of 6846 steelworkers, aged 22-60 years, from the baseline survey of a Chinese occupational cohort.

Primary And Secondary Outcome Measures: Different exposure metrics of night shift work, including current shift status, duration of night shifts (years), cumulative number of night shifts (nights), cumulative length of night shifts (hours), average frequency of night shifts (nights/month), average length of night shifts (hours/night) and percentage of hours on night shifts, were used to examine the effects of past and current night shift work on HHcy odds. The total homocysteine concentration in the plasma above 15 µmol/L was defined as HHcy.

Results: Compared with those who never worked night shifts, current night shift workers had elevated odds of HHcy (OR 1.23, 95% CI 1.06 to 1.44). Considering a person's lifetime work schedule and compared with individuals who never worked night shifts, duration of night shifts >28 years (OR 1.35, 95% CI 1.12 to 1.61), average frequency of night shifts >7 nights/month (OR 1.25, 95% CI 1.07 to 1.47) and percentage of hours on night shifts >30% (OR 1.23, 95% CI 1.05 to 1.43) were associated with higher HHcy odds. The duration of night shifts >20 years and the average frequency of night shifts >7 nights/month could significantly increase the odds of HHcy regardless of whether the average length of night shifts was greater than 8 hours/night. After stratification by sex, no significant association was found in female workers between different exposure metrics of night shift work and HHcy.

Conclusions: Long duration and high frequency of night shift work are associated with higher HHcy odds among male steelworkers.
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http://dx.doi.org/10.1136/bmjopen-2020-041576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745529PMC
December 2020

Cysteine-enhanced reductive degradation of nitrobenzene using nano-sized zero-valent iron by accelerated electron transfer.

J Environ Sci (China) 2021 Feb 24;100:110-116. Epub 2020 Jul 24.

College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China. Electronic address:

As an aliphatic amino acid, cysteine (CYS) is diffuse in the living cells of plants and animals. However, little is known of its role in the reactivity of nano-sized zero-valent iron (NZVI) in the degradation of pollutants. This study shows that the introduction of CYS to the NZVI system can help improve the efficiency of reduction, with 30% more efficient degradation and a reaction rate constant nine times higher when nitrobenzene (NB) is used as probe compound. The rates of degradation of NB were positively correlated with the range of concentrations of CYS from 0 to 10 mmol/L. The introduction of CYS increased the maximum concentration of Fe(III) by 12 times and that of Fe(II) by four times in this system. A comparison of systems featuring only CYS or Fe(II) showed that the direct reduction of NB was not the main factor influencing its CYS-stimulated removal. The reduction in the concentration of CYS was accompanied by the generation of cystine (CY, the oxidized form of cysteine), and both eventually became stable. The introduction of CY also enhanced NB degradation due to NZVI, accompanied by the regeneration of CYS. This supports the claim that CYS can accelerate electron transfer from NZVI to NB, thus enhancing the efficiency of degradation of NB.
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http://dx.doi.org/10.1016/j.jes.2020.07.017DOI Listing
February 2021

Impact of green reduced graphene oxide on sewage sludge bioleaching with Acidithiobacillus ferrooxidanse.

Environ Pollut 2020 Dec 26;267:115455. Epub 2020 Aug 26.

Fujian Key Laboratory of Pollution Control and Resource Reuse, School of Environment Science and Engineering, Fujian Normal University, Fuzhou, 350007, Fujian, China. Electronic address:

Worldwide graphene use is rapidly increasing in a variety of industrial applications to such an extent that efflux into the environment seems inevitable where the likely final reservoirs of graphene wastes is likely to be wastewater treatment plants are. Despite this an understanding of how graphene products impact the bioleaching of metals from sludge is still limited. In this study, the effect of reduced graphene oxide synthesized from eucalyptus leaf extracts (EL-rGO) on Zn and Cu bioleaching from sludge was investigated. The major new findings were that EL-rGO had a negative effect on Acidithiobacillus ferrooxidans (A. ferrooxidans) growth; since optical density decreased by 0.059 as EL-rGO dose increased from 1 to 50 mg/L, and the bioleaching of Cu and Zn decreased by 27.7 and 20.2%, respectively. While at a EL-rGO dose of 1 mg/L A. ferrooxidans grew better, scanning electron microscopy (SEM) confirmed that exposure to EL-rGO caused cell membrane disruption at 50 mg/L. Cytotoxicity tests showed that this was related to an increase in lactate dehydrogenase (LDH) release rate and a decrease in superoxide dismutase (SOD) activity. These new findings provide evidence that green synthesized rGO is toxic to microorganisms and that toxicity increased with rGO dose.
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http://dx.doi.org/10.1016/j.envpol.2020.115455DOI Listing
December 2020

Sediment Records of Polybrominated Diphenyl Ethers (PBDEs) from the Anhui Province Section of Yangtze River, China.

Bull Environ Contam Toxicol 2021 Feb 26;106(2):334-341. Epub 2020 Nov 26.

School of Biology, Food and Environment, Hefei University, Hefei, 230022, Anhui Province, China.

This study investigated the temporal changes of PBDEs in the sediment cores from the Anhui Province section of Yangtze River (YR), China. The dramatic increase of ∑Br-Br-BDEs and BDE-209 concentrations in cores in the period of ca. 1990-2008 was consistent with the booming of regional and national production and consumption of household appliances and electronics. Following declines in BDE-209 and ∑Br-Br-BDEs concentrations can be associated with the deceasing use of legacy commercial PBDE mixtures in recent years. Compared with the different regions around the world, sediments in the YR were moderately polluted by PBDEs. The levels and the detection rates of BDE-209 were highest among the detected PBDEs single congener. The composition analysis indicated that Deca-BDE, Octa-BDE, and Penta-BDE were the dominant forms in the YR. The increasing trends of both BDE-209 and ∑Br-Br-BDEs in the YR during 1990s and 2000s largely reflected the time periods for transferring PBDE sources from the developed countries to China. TOC and finer particles were strongly correlated with distributions of PBDEs in sediments.
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http://dx.doi.org/10.1007/s00128-020-03054-xDOI Listing
February 2021

Inter-trophic Interaction of Gut Microbiota in a Tripartite System.

Microb Ecol 2021 May 14;81(4):1075-1087. Epub 2020 Nov 14.

College of Life Sciences, Qufu Normal University, Qufu, 273165, China.

Gut microbiota can be transmitted either environmentally or socially and vertically at intraspecific level; however, whether gut microbiota interact along trophic levels has been largely overlooked. Here, we characterized the gut bacterial communities of weevil larvae of Curculio arakawai that infest acorns of Mongolian oak (Quercus mongolica) as well as acorn-eating mammals, Siberian chipmunk (Tamias sibiricus), to test whether consumption of seed-borne larvae remodels the gut bacterial communities of T. sibiricus. Ingestion of weevil larvae of C. arakawai significantly altered the gut bacterial communities of T. sibiricus. Consequently, T. sibiricus fed larvae of C. arakawai showed higher capability to counter the negative effects of tannins, in terms of body weight maintenance, acorn consumption, N content in feces, urine pH, and blood ALT activity. Our results may first show that seed-borne insects as hidden players have a potential to alter the gut microbiota of seed predators in the tripartite system.
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http://dx.doi.org/10.1007/s00248-020-01640-4DOI Listing
May 2021

Association between serum lactate levels and enteral feeding intolerance in septic patients treated with vasopressors: a retrospective cohort study.

Ann Transl Med 2020 Oct;8(19):1240

Department of Critical Care Medicine, the First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Background: To assess the association between serum lactate levels and intolerance to enteral nutrition (EN) in septic patients treated with vasopressors.

Methods: This retrospective study was conducted between January 1, 2015 and May 1, 2018 in an intensive care unit (ICU). Patients with sepsis who were given EN and treated with vasopressors were included. EmpowerStats software and R (version 3.3.2) was used to examine the association between serum lactate levels and intolerance to EN.

Results: Among the 132 septic patients (age, 60.6±18.1 years) enrolled, 35 (26.5%) patients suffered intolerance to EN. Multiple logistic regression analysis demonstrated that an elevated lactate level was an independent risk factor for EN intolerance [odds ratio (OR): 2.7; 95% confidence interval (CI): 1.6-4.4; P<0.001]. The area under the receiver operating characteristic (ROC) curve for serum lactate levels was 0.764 (95% CI: 0.664-0.864). Stratified analysis suggested that age was the most prominent interactive factor for serum lactate levels in EN intolerance. Serum lactate levels were closely correlated to EN intolerance in elderly patients (age ≥65 years) (OR: 9.5; 95% CI: 2.1-42.4; P=0.0261 for interaction), while no such association was identified in younger patients (age <65 years; OR: 1.7; 95% CI: 1.0-2.9; P=0.052).

Conclusions: Serum lactate levels were associated with an increased risk of EN intolerance in patients with sepsis, especially in elderly individuals. An elevated serum lactate level may be an early predictor of EN intolerance in elderly septic patients treated with vasopressors. However, further studies are called for to verify these findings.
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http://dx.doi.org/10.21037/atm-20-6317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607076PMC
October 2020

Clinical characteristics and prognostic implications of BRCA-associated tumors in males: a pan-tumor survey.

BMC Cancer 2020 Oct 14;20(1):994. Epub 2020 Oct 14.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, P. R. China, 510060.

Background: The BRCA mutation (BRCAm) in males has been reported to confer a higher risk for the development of various tumors. However, little is known about its clinicopathologic features and prognostic implications.

Design: We conducted a retrospective pan-tumor survey on 346 cases of BRCA-associated tumors in males. Comparative analyses were conducted among male and female patients with BRCAm (n = 349), as well as in male patients without BRCAm (n = 4577).

Results: Similar incidences of BRCAm (6.0 vs. 6.6%) and age at diagnosis of tumor (median, 65 vs. 60 years) were observed in male and female patients. Carcinomas of the lung, bladder, stomach, and cutaneous melanoma were the frequent tumors demonstrating BRCAm in males, of which the majority were stage II or III diseases with a higher frequency of BRCA2 mutations. Compared to that in the non-BRCAm group, cutaneous melanoma (16.3 vs. 5.0%), lung cancer (19.4 vs. 11.8%), bladder cancer (15.6 vs. 5.6%), and stomach cancer (11.9 vs. 5.5%) accounted for a higher proportion in the BRCAm group. Advanced disease and more mutation counts (median, 322 vs. 63 mutations) were also found in the BRCAm group. A total of 127 BRCA1 and 311 BRCA2 mutations were identified, of which 21.8 and 28.6% were deleterious, respectively. Frequent deleterious variants were identified in carcinomas of the breast (100.0%), colorectum (62.2%), prostate (43.3%), and stomach (42.9%). BRCA1 fusions with NF1, FAM134C, BECN1, or LSM12 and recurrent BRCA2 mutations at P606L/S, E832K/G, and T3033Lfs*29 were detected. Frameshift mutations in BRCA2 at N1784 (N1784Kfs*3, N1784Tfs*3) were frequently observed in both male and female patients. Compared with those in females, BRCA mutations in males were associated with decreased overall survival (OS) and progression-free survival (PFS). Male patients with deleterious BRCAm displayed increased OS compared with non-BRCAm carriers. The subgroup analysis demonstrated that BRCAm was associated with increased OS in gastric and bladder cancers, decreased PFS in prostate, esophageal, and head and neck cancers, and decreased OS in glioma/glioblastoma in males.

Conclusion: These findings provide an overview of the distinct characteristics and clinical outcomes of male patients with BRCA-associated tumors, suggesting the importance of further genetic BRCA testing in males.
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http://dx.doi.org/10.1186/s12885-020-07481-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556962PMC
October 2020

Ibrutinib in B-cell lymphoma: single fighter might be enough?

Cancer Cell Int 2020 29;20:467. Epub 2020 Sep 29.

Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No.324, Jingwu Road, 250021 Jinan, Shandong China.

Background: In recent years, the B cell receptor (BCR) signaling pathway has become a "hot point" because it plays a critical role in B-cell proliferation and function. Bruton's tyrosine kinase (BTK) is overexpressed in many subtypes of B-cell lymphoma as a downstream kinase in the BCR signaling pathway. Ibrutinib, the first generation of BTK inhibitor, has shown excellent antitumor activity in both indolent and aggressive B-cell lymphoma.

Main Body: Ibrutinib monotherapy has been confirmed to be effective with a high response rate (RR) and well-tolerated in many B-cell lymphoma subgroups. To achieve much deeper and faster remission, combination strategies contained ibrutinib were conducted to evaluate their synergistic anti-tumor effect.

Conclusions: For patients with indolent B-cell lymphoma, most of them respond well with ibrutinib monotherapy. Combination strategies contained ibrutinib might be a better choice to achieve deeper and faster remission in the treatment of aggressive subtypes of B-cell lymphoma. Further investigations on the long-term efficacy and safety of the ibrutinib will provide novel strategies for individualized treatment of B-cell lymphoma.
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http://dx.doi.org/10.1186/s12935-020-01518-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523373PMC
September 2020

A Tandem 0D/2D/2D NbS Quantum Dot/Nb O Nanosheet/g-C N Flake System with Spatial Charge-Transfer Cascades for Boosting Photocatalytic Hydrogen Evolution.

Small 2020 Oct 23;16(42):e2003302. Epub 2020 Sep 23.

School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, 610054, China.

The relatively high recombination rate of charges remains the most critical limiting factor for solar-driven water splitting for hydrogen generation. Herein, a tandem 0D/2D/2D NbS quantum dot/Nb O nanosheet/g-C N flake (NSNOCN) system is designed. Owing to the unique spatial-arrangement and elaborate morphology of 0D NbS , 2D Nb O , and 2D g-C N in the newly designed NSNOCN, plenty of spatial charge-transfer cascades from g-C N to NbS via Nb O are formed to accelerate separation and transfer of charges significantly, thus contributing to a high photocatalytic H generation rate of 13.99 mmol h g (an apparent quantum efficiency of 10.8% at 420 nm), up to 107.6 and 43.7 times by contrast with that of g-C N and Nb O , respectively. This work can provide a new platform in the design of artificial photocatalytic systems with high charge-transfer efficiency.
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http://dx.doi.org/10.1002/smll.202003302DOI Listing
October 2020

TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells.

J Biol Chem 2020 11 22;295(48):16314-16327. Epub 2020 Sep 22.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:

TNF ligand-related molecule 1A (TL1A) is a vascular endothelial growth inhibitor to reduce neovascularization. Lack of apoE a expression results in hypercholesterolemia and atherosclerosis. In this study, we determined the precise effects of TL1A on the development of atherosclerosis and the underlying mechanisms in apoE-deficient mice. After 12 weeks of pro-atherogenic high-fat diet feeding and TL1A treatment, mouse aorta, serum, and liver samples were collected and used to assess atherosclerotic lesions, fatty liver, and expression of related molecules. We found that TL1A treatment significantly reduced lesions and enhanced plaque stability. Mechanistically, TL1A inhibited formation of foam cells derived from vascular smooth muscle cells (VSMCs) but not macrophages by activating expression of ABC transporter A1 (ABCA1), ABCG1, and cholesterol efflux in a liver X receptor-dependent manner. TL1A reduced the transformation of VSMCs from contractile phenotype into synthetic phenotypes by activating expression of contractile marker α smooth muscle actin and inhibiting expression of synthetic marker osteopontin, or osteoblast-like phenotype by reducing calcification. In addition, TL1A ameliorated high-fat diet-induced lipid metabolic disorders in the liver. Taken together, our work shows that TL1A can inhibit the development of atherosclerosis by regulating VSMC/foam cell formation and switch of VSMC phenotypes and suggests further investigation of its potential for atherosclerosis treatment.
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http://dx.doi.org/10.1074/jbc.RA120.015486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705304PMC
November 2020

Adiponectin agonist ADP355 ameliorates doxorubicin-induced cardiotoxicity by decreasing cardiomyocyte apoptosis and oxidative stress.

Biochem Biophys Res Commun 2020 12 18;533(3):304-312. Epub 2020 Sep 18.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:

Doxorubicin (DOX) is an anthracycline derivative and widely used as an anticancer drug. However, the severe cardiotoxicity of DOX limits its application. ADP355 is an adiponectin-based active peptide with anti-liver fibrosis and atherosclerosis properties. It remains unclear the effects and involved mechanisms of ADP355 in DOX-induced cardiotoxicity. C57BL/6J mice were intraperitoneally injected DOX once a week to induce heart failure while receiving ADP355 treatment daily for 4 weeks. At the end of experiment, blood and heart tissues were collected. We found that ADP355 markedly improved DOX-induced cardiac dysfunction and histopathological damage, and decreased serum creatine kinase, lactate dehydrogenase and hydroxybutyrate dehydrogenase levels. The anti-apoptotic activity of ADP355 was indicated by reduction in TUNEL-positive cells and cleaved caspase-3 expression, along with decreased BCL2-associated X protein/B cell lymphoma 2 (BAX/BCL2) levels in heart tissues. Additionally, ADP355 markedly increased DOX-decreased cell viability by reducing BAX/BCL2, but inhibited reactive oxygen species production in H9c2 cells. Mechanistically, ADP355 attenuated expression of DOX-reduced nuclear factor-erythroid 2-related factor 2 (Nrf2) and superoxide dismutase 2, as well as mRNA levels of Nrf2 downstream targets. Furthermore, ADP355 activated sirtuin 2 and its target genes. In conclusion, we demonstrate that ADP355 alleviates DOX-induced cardiotoxicity by inhibiting myocardial apoptosis and oxidative stress through Nrf2 and sirtuin 2 signaling pathways. These findings suggest that ADP355 can be a promising candidate for the treatment of cardiac dysfunction.
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http://dx.doi.org/10.1016/j.bbrc.2020.09.035DOI Listing
December 2020

Surgical treatment of spinal thoracic metastases with nerve injury in patients with moderate-to-severe spinal cord injury.

Ann Palliat Med 2020 Sep 11;9(5):3204-3212. Epub 2020 Sep 11.

Orthopedics Department of the First Medical Center in PLA General Hospital, Beijing, China.

Background: Among spine metastases of malignant tumors, thoracic spine metastases account for about 70%. Spinal metastases cause spinal instability, compression of nerve structures, and function damage, which has a serious impact on patients' quality of life (QOL). At present, surgery is main choice in the treatment of spinal metastases. However, conventional surgery still has certain limitations. This study explored the surgical strategy of nerve rescue in patients with spinal thoracic metastases and moderate-to-severe spinal cord injury.

Methods: In this history case-control study, 42 patients received conventional operation were enrolled as control group, while 38 patients who underwent conventional decompression of laminectomy combined with durotomy were selected as observation group. Perioperative data were recorded for comparisons between the two groups. Visual analogue scale (VAS) of pain, QOL, and 36-item short-form health survey (SF-36) were compared before operation and 3, 6, and 12 months after operation. American Spinal Injury Association (ASIA) grade was evaluated before operation and 1 month after. Complications, recurrence rate, and mortality were also recorded.

Results: The VAS scores of the observation group at 3, 6, and 12 months after operation were significantly lower than those before the operation. The QOL and SF-36 scores were increased compared with those taken preoperatively (P<0.05). The VAS score of the observation group was lower than that of the control group, and the QOL and SF-36 scores were higher than those in the control group (P<0.05). The neurological grades of ASIA 3 months after operation in both groups were significantly improved. The improvement in the observation group was greater than that in the control group (P<0.05). The incidences of postoperative complications in the control group and observation group were 19.05% (8/42) and 7.89% (3/38), respectively; the recurrence rates of the two groups were 14.29% (6/42) and 5.26% (2/38), respectively; and the mortality rates of the two groups were 26.19% (11/42) and 18.42%, respectively (7/38) (P>0.05).

Conclusions: Durotomy based on conventional decompression of laminectomy can effectively save nerve function in metastases of the thoracic spine with moderate or severe spinal cord injury, improve QOL, and is thus worthy of being applied in clinic.
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http://dx.doi.org/10.21037/apm-20-1507DOI Listing
September 2020