Publications by authors named "Chao Wei"

331 Publications

Optimization of a novel lipid extraction process from microalgae.

Sci Rep 2021 Oct 12;11(1):20221. Epub 2021 Oct 12.

Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, China.

Previous study found that the solvent extraction efficiency of lipid in microalgae could be greatly improved by washing algae cells before the second time extraction. Based on the "organic solvents-water-organic solvents" method, this research further studied the effect of four solvent systems (acetone, chloroform/methanol, chloroform/methanol/water, dichloromethane/methanol), two types of water treatment (vortex and ultrasonic), three water treatment time gradient (0 s, 30 s, 120 s) on the lipid extraction at three different microalgae growth stages (3rd day, 5th day, 9th day). The results show that the combination of water treatment type, treatment time and solvent is very important to the efficiency of lipid extraction. The total lipid extracted was generally increased by 10-30% after water treatment. Especially under the condition of 120 s vortex water treatment with dichloromethane/methanol as extraction solvent, the total lipid extracted increased by 61.14%. In addition, microalgae cells at different culture stages had different sensitivity to water treatment. In this study, under the combination of chloroform/methanol/water as extraction solvent and vortex water treatment for 120 s, the highest lipid yield was obtained on the ninth day of cell culture, which accounts 47.88% of the cell dry weight (478 mg/g cell dry weight). The changes of cell morphology and structure after water treatment were studied by scanning electron microscope, and it was found that water treatment could seriously destroy the cell membrane damaged by solvent, thus promoting the release of lipids. This study further optimizes the "solvent-water-solvent" lipid extraction method, which neither produces impurities nor damages the lipid quality, and can reduce the amount of organic solvent applied in the classical lipid extraction method with the same lipid yield, so it has a broad application prospect.
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http://dx.doi.org/10.1038/s41598-021-99356-zDOI Listing
October 2021

Research on Urban Spatial Connection and Network Structure of Urban Agglomeration in Yangtze River Delta-Based on the Perspective of Information Flow.

Int J Environ Res Public Health 2021 Sep 29;18(19). Epub 2021 Sep 29.

Department of Economics, School of Economics and Management, China University of Geosciences, Wuhan 430079, China.

Exploration of urban spatial connections and network structures of urban agglomeration in the Yangtze River Delta, as well as its influencing factors, is of great significance regarding optimization of the development pattern of the Yangtze River Delta urban agglomeration and promotion of regional high-quality development. Therefore, based on Baidu index data in 2015 and 2019, this paper first analyzes the spatiotemporal variation characteristics of information-flow connections in the Yangtze River Delta urban agglomeration. Then it uses social network analysis to explore the information-flow network structure in the Yangtze River Delta urban agglomeration, and finally explores the influencing factors of information-flow intensity in the Yangtze River Delta urban agglomeration. The main conclusions are as follows: (1) The total amount of information flow in the Yangtze River Delta urban agglomeration has had no obvious change, and the coverage of information flow in the central urban circle has expanded. (2) The network hierarchy presents a relatively stable "pyramid" distribution pattern, which tends to develop into a "spindle" pattern. (3) The overall network density of the Yangtze River Delta urban agglomeration is high and is increasing. The backbone network is a "triangle" structure. The central cities in the region are stable, and the subgroups are adjacent to each other geographically. (4) Gross Domestic Product, resident population of the region and the number of Internet broadband subscribers all have important effects on the total information flow, among which the number of Internet broadband subscribers has the greatest effect on the total information flow. In addition, urban functions and their positioning, urban events, history and culture, and other factors that are difficult to quantify also have a certain impact on the information-flow network among cities.
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http://dx.doi.org/10.3390/ijerph181910288DOI Listing
September 2021

[Research Progress of in Vitro Experiment of Allergic Transfusion Reaction for Plasma Transfusion and Its Relative Mechanism---Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Oct;29(5):1662-1666

Department of Blood Transfusion, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China E-mail:

Allergic transfusion reaction (ATR) caused by plasma transfusion is one of the main adverse transfusion reactions, and severe allergic reactions may even endanger the patient's life. Currently, ATR is mainly prevented and controlled by drug prevention and symptomatic treatment, and there still lack of preventive measures such as in vitro experiments. It has been shown that mast cells and basophils are the main effector cells of allergic reactions, and histamine is one of the main mediators of IgE-mediated allergic reactions. Some experiments can be used to identify patients with allergies or plasma components containing allergens, such as detection of serum-specific IgE, IgA, anti-IgA antibody, tryptase and histamine, mast cell degranulation test, basophil activation test, and so on. The basophil activation test can also be used for functional matching of plasma in vitro. Research of in vitro experiment of ATR is good for directing the precise infusion of plasma, reducing waste of resources, and avoiding the risk of blood transfusion. As a pre-transfusion laboratory test for clinical use, in vitro experiment of functional matching provides a new way to prevent ATR.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.05.047DOI Listing
October 2021

Analysis of pre-operative risk factors for post-operative urosepsis following mini-percutaneous nephrolithotomy in patients with large kidney stones.

J Endourol 2021 Sep 25. Epub 2021 Sep 25.

Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, 66375, Urology, No. 1095, Jiefang Avenue, Wuhan, China, 430030;

Purpose: To assess the pre-operative risk factors for post-operative urosepsis following mini-percutaneous nephrolithotomy (mPCNL) in patients with large kidney stones. Methods:Records of 171 patients with large (≥30mm) kidney stones who underwent mPCNL from December 2013 to October 2019 were reviewed. Demographic data of patients, pre-operative urine analysis, urine culture, routine blood tests and abdominal computerized cosmography data were collected and analyzed. A predictive nomogram model was established based on the results of logistic regression.

Results: 29 patients (17%) developed post-operative urosepsis in this study. Univariate analysis demonstrated that preoperative urine leukocytes (P <0.001), urine nitrite (P <0.001), stones in adjacent calices on the coronal plane (P <0.001), the maximum cross-sectional area of stones (P <0.001), the diameter of hydronephrosis (P =0.010) and number of stones (P=0.044) were associated with post-operative urosepsis after mPCNL in patients with large kidney stones. And pre-operative urine leukocytes ≥450/ µl (P=0.002) was the only independent risk factor for post-operative urosepsis in multivariate logistic regression analysis. Based on the results of multivariate regression, a nomogram model was established for the prediction of post-operative urosepsis with ideal discrimination (area under receiver operating characteristic curve was 0.867).

Conclusion: Patients with certain pre-operative characteristics, including higher urine leukocytes, positive urine nitrite, stones in adjacent calices on the coronal plane, larger maximum cross-sectional area of stones, larger diameter of hydronephrosis and larger number of stones, who received mPCNL may have a higher risk of postoperative urosepsis. A predictive model can help urologists identify patients who may develop post-operative urosepsis with high probability.
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http://dx.doi.org/10.1089/end.2021.0406DOI Listing
September 2021

Emotional Intelligence and Prosocial Behavior in College Students: A Moderated Mediation Analysis.

Front Psychol 2021 6;12:713227. Epub 2021 Sep 6.

School of Psychology, Northeast Normal University, Changchun, China.

This study examined the relationship between emotional intelligence (EI) and prosocial behavior (PSB) and constructed a model for their interaction by examining the mediating effect of social support (SS) and the moderating effect of self-esteem (SE) in this relationship. A total of 742 college students aged from 18 to 20 in Northeast China ( =19.42 ± 0.53 years) completed a survey measuring the Emotional Intelligence Scale, Prosocial Tendencies Measurement Scale-Chinese Version, Perceived Social Support Scale, and Self-Esteem Scale. The results showed that: (1) EI positively predicted PSB; (2) SS partially mediated the relationship between EI and PSB; and (3) SE moderated the direct effect of EI on PSB and the relationship between SS and PSB. That is, when the SE of college students was higher, the effect of SS in promoting PSB was enhanced. Therefore, our results suggested that under the influence of both internal and external factors, there is an indirect effect of EI on PSB. This finding may potentially provide a theoretical basis for designing college students' mental health courses and cultivating PSB in college.
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http://dx.doi.org/10.3389/fpsyg.2021.713227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450319PMC
September 2021

The PdeK-PdeR two-component system promotes unipolar localization of FimX and pilus extension in pv. .

Sci Signal 2021 Sep 14;14(700):eabi9589. Epub 2021 Sep 14.

Department of Plant Pathology and MOA Key Laboratory of Pest Monitoring and Green Management, and Joint International Research Laboratory of Crop Molecular Breeding, Ministry of Education, China Agricultural University, Beijing 100193, China.

[Figure: see text].
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http://dx.doi.org/10.1126/scisignal.abi9589DOI Listing
September 2021

Pyruvate-Driven Oxidative Phosphorylation is Downregulated in Sepsis-Induced Cardiomyopathy: A Study Of Mitochondrial Proteome.

Shock 2021 Sep 9. Epub 2021 Sep 9.

Translational Research Program, Department of Anesthesiology & Center for Shock, Trauma and Anesthesiology Research, Baltimore, MD, USA The Department of Physiology and Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD, USA Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA.

Background: Sepsis-induced cardiomyopathy (SIC) is a major contributing factor for morbidity and mortality in sepsis. Accumulative evidence has suggested that cardiac mitochondrial oxidative phosphorylation is attenuated in sepsis, but the underlying molecular mechanisms remain incompletely understood.

Methods: Adult male mice of 9-12 weeks old were subjected to sham or cecal ligation and puncture procedure. Echocardiography in vivo and Langendorff-perfused hearts were used to assess cardiac function 24 hours after the procedures. Unbiased proteomics analysis was performed to profile mitochondrial proteins in the hearts of both sham and SIC mice. Seahorse respirator technology was used to evaluate oxygen consumption in purified mitochondria.

Results: Of the 665 mitochondrial proteins identified in the proteomics assay, 35 were altered in septic mice. The mitochondrial remodeling involved various energy metabolism pathways including subunits of the electron transport chain, fatty acid catabolism, and carbohydrate oxidative metabolism. We also identified a significant increase of pyruvate dehydrogenase (PDH) kinase 4 (PDK4) and inhibition of PDH activity in septic hearts. Furthermore, compared to sham mice, mitochondrial oxygen consumption of septic mice was significantly reduced when pyruvate was provided as a substrate. However, it was unchanged when PDH was bypassed by directly supplying the Complex I substrate NADH, or by using the Complex II substrate succinate, or using Complex IV substrate, or by providing the beta-oxidation substrate palmitoylcarnitine, neither of which require PDH for mitochondrial oxygen consumption.

Conclusions: These data demonstrate a broad mitochondrial protein remodeling, PDH inactivation and impaired pyruvate-fueled oxidative phosphorylation during SIC, and provide a molecular framework for further exploration.
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http://dx.doi.org/10.1097/SHK.0000000000001858DOI Listing
September 2021

Correction for: TGF-β and NF-κB signaling pathway crosstalk potentiates corneal epithelial senescence through an RNA stress response.

Aging (Albany NY) 2021 08 31;13(16):20853. Epub 2021 Aug 31.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.

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http://dx.doi.org/10.18632/aging.203516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436941PMC
August 2021

Therapeutic Potential of Extracellular Vesicles for Sepsis Treatment.

Adv Ther (Weinh) 2021 Jul 29;4(7). Epub 2021 Apr 29.

Fischell Department of Bioengineering and Program in Molecular and, Cell Biology, University of Maryland, 3102 A. James Clark Hall, College Park, MD 20742, USA.

Sepsis is a deadly condition lacking a specific treatment despite decades of research. This has prompted the exploration of new approaches, with extracellular vesicles (EVs) emerging as a focal area. EVs are nanosized, cell-derived particles that transport bioactive components (i.e., proteins, DNA, and RNA) between cells, enabling both normal physiological functions and disease progression depending on context. In particular, EVs have been identified as critical mediators of sepsis pathophysiology. However, EVs are also thought to constitute the biologically active component of cell-based therapies and have demonstrated anti-inflammatory, anti-apoptotic, and immunomodulatory effects in sepsis models. The dual nature of EVs in sepsis is explored here, discussing their endogenous roles and highlighting their therapeutic properties and potential. Related to the latter component, prior studies involving EVs from mesenchymal stem/stromal cells (MSCs) and other sources are discussed and emerging producer cells that could play important roles in future EV-based sepsis therapies are identified. Further, how methodologies could impact therapeutic development toward sepsis treatment to enhance and control EV potency is described.
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http://dx.doi.org/10.1002/adtp.202000259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378673PMC
July 2021

A Nonlethal Murine Flame Burn Model Leads to a Transient Reduction in Host Defenses and Enhanced Susceptibility to Lethal Pseudomonas aeruginosa Infection.

Infect Immun 2021 Sep 21;89(10):e0009121. Epub 2021 Jun 21.

Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Of the 486,000 burn injuries that required medical treatment in the United States in 2016, 40,000 people were hospitalized, with >3,000 fatalities. After burn injury, humans are at increased risk of sepsis and mortality from infections caused by Pseudomonas aeruginosa, an opportunistic pathogen. We hypothesize that systemic events were initiated from the burn that increased the host's susceptibility to P. aeruginosa. A nonlethal 10% total body surface area (TBSA), full-thickness flame burn was performed in CD-1 mice without and with subsequent P. aeruginosa (strain M2) infection. The 50% lethal dose for subcutaneous infection with P. aeruginosa M2 at the burn site immediately after the burn decreased by 6 log, with mortality occurring between 18 and 26 h, compared with P. aeruginosa-infected mice without burn injury. Bacteria in distal organs were detected by 18 h, concurrent with the onset of clinical symptoms. Serum proinflammatory cytokines (interleukin-6 [IL-6], IL-1β, gamma interferon, and tumor necrosis factor alpha) and the anti-inflammatory cytokine IL-10 were first detected at 12 h postburn with infection and continued to increase until death. Directly after burn alone, serum levels of HMGB1, a danger-associated molecular pattern and TLR4 agonist, transiently increased to 50 ng/ml before returning to 20 ng/ml. Burn with P. aeruginosa infection increased serum HMGB1 concentrations >10-fold (250 ng/ml) at the time of death. This HMGB1-rich serum stimulated TLR4-mediated NF-κB activation in a TLR4 reporter cell line. Treatment of infected burned mice with P5779, a peptide inhibitor of HMGB1, increased the mean survival from 23 to 42 h ( < 0.0001). We conclude that the high level of serum HMGB1, which preceded the increase in proinflammatory cytokines, is associated with postburn mortality.
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http://dx.doi.org/10.1128/IAI.00091-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445176PMC
September 2021

Molecular Targets and Mechanisms of - Drug Pair for the Treatment of Ulcerative Colitis Based on Network Pharmacology and Molecular Docking.

Evid Based Complement Alternat Med 2021 4;2021:9929093. Epub 2021 Jun 4.

College of Integrated Chinese and Western Medicine, Jining Medical University, Jining 272067, China.

This study aims to analyze the targets of the effective active ingredients of - drug pair (SCDP) in ulcerative colitis (UC) by network pharmacology and molecular docking and to explore the associated therapeutic mechanism. The effective active ingredients and targets of SCDP were determined from the TCMSP database, and the drug ingredient-target network was constructed using the Cytoscape software. The disease targets related to UC were searched in GeneCards, DisGeNET, OMIM, and DrugBank databases. Then, the drug ingredient and disease targets were intersected to construct a protein-protein interaction network through the STRING database. The Metascape database was used for the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the predicted targets of SCDP for UC. The Autodock software was used for molecular docking between the main active ingredient and the core target to evaluate the binding ability. SCDP has 43 effective active ingredients and 134 intersection targets. Core targets included AKT1, TP53, IL-6, VEGFA, CASP3, JUN, TNF, MYC, EGFR, and PTGS2. GO functional enrichment analysis showed that biological process was mainly associated with a cytokine-mediated signaling pathway, response to an inorganic substance, response to a toxic substance, response to lipopolysaccharide, reactive oxygen species metabolic process, positive regulation of cell death, apoptotic signaling pathway, and response to wounding. KEGG enrichment analysis showed main pathway concentrations were related to pathways in cancer, AGE-RAGE signaling pathway in diabetic complications, bladder cancer, IL-17 signaling pathway, apoptosis, p53 signaling pathway, and PI3K-Akt signaling pathway. The drug active ingredient-core target-key pathway network contains 41 nodes and 108 edges, of which quercetin, wogonin, baicalein, acacetin, oroxylin A, and beta-sitosterol are important active ingredients; PTGS2, CASP3, TP53, IL-6, TNF, and AKT1 are important targets; and the pathways involved in UC treatment include pathways in cancer, PI3K-Akt signaling pathway, AGE-RAGE signaling pathway in diabetic, apoptosis, IL-17 signaling pathway and herpes simplex infection. The active ingredient has a good binding capacity to the core target. SCDP key active ingredients are mainly quercetin, wogonin, baicalein, acacetin, oroxylin A, and beta-sitosterol, which function mainly by regulating targets, such as PTGS2, CASP3, TP53, IL-6, TNF, and AKT1, and are associated with multiple signaling pathways as pathways in cancer, PI3K-Akt signaling pathway, apoptosis, IL-17 signaling pathways.
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http://dx.doi.org/10.1155/2021/9929093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195671PMC
June 2021

Lower Urinary Tract Symptoms and Sexual Dysfunction in Male: A Systematic Review and Meta-Analysis.

Front Med (Lausanne) 2021 28;8:653510. Epub 2021 May 28.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

An association between lower urinary tract symptoms (LUTS) and risk of sexual dysfunction in male remains controversial in recent decades. PubMed and Web of Science were searched up to October 28, 2020, for articles reporting the prevalence of sexual dysfunction in men with LUTS. The main outcomes were results from sexual dysfunction assessments. Pooled odds ratio (OR) and weighted mean difference (WMD) with 95% confidence interval (CI) were calculated. The quality assessment of the included studies was performed by using The Newcastle-Ottawa Scale (NOS) or JBI Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI). A total of 24 full-manuscript papers met the inclusion criteria. The pooled OR for 21 studies suggested that patients with severer LUTS had a higher risk of sexual dysfunction (OR = 3.31, 95% CI: 2.43 to 4.49, < 0.001, = 90%). A significant decrease in scores of assessment tools for sexual dysfunction was observed in the patients with higher severity of LUTS compared with those patients with lower severity (WMD = -5.49, 95%CI: -7.25 to -3.27, P < 0.001, = 96%). Similar outcomes were also found in subgroup analyses. In a detailed analysis of specific sexual function domains, the severity of LUTS was associated with erectile dysfunction, intercourse satisfaction, and overall satisfaction, except for sexual desire. The study demonstrates an association between exposure of lower urinary tract symptoms and risk of sexual dysfunction in male. Assessment of sexual function is necessary for patients with lower urinary tract symptoms. http://www.crd.york.ac.uk/prospero, identifier: CRD42020208747.
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http://dx.doi.org/10.3389/fmed.2021.653510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193225PMC
May 2021

The expression profiles and prognostic values of HSP70s in hepatocellular carcinoma.

Cancer Cell Int 2021 May 31;21(1):286. Epub 2021 May 31.

Hepato-Biliary-Pancreatic Surgery Division, Department of General Surgery, The Second Affiliated Hospital of Nanchang University, No. 1, Minde Road, Nanchang, 330006, China.

Background: The HSP70 family of heat shock protein plays a critical role in protein synthesis and transport to maintain protein homeostasis. Several studies have indicated that HSP70s are related to the development and occurrence of various cancers.

Methods: The relationship between the overall survival rate of hepatocellular carcinoma patients and the expression of 14 HSP70s from multiple databases, such as TCGA, ONCOMINE, cBioPortal was investigated. Western Blot and PCR were used to evaluate HSPA4 and HSPA14 expressions in various HCC cells to identify suitable cell lines for further experiments .Wound-healing assays, Transwell assays and EdU assays were used to verify the effects of HSPA4 and HSPA14 on the function of hepatocellular carcinoma cells, and statistical analysis was performed.

Results: Hepatocellular carcinoma tissues significantly expressed the 14 HSP70s compared to the normal samples. Besides, the high HSPA1A, HSPA1B, HSPA4, HSPA5, HSPA8, HSPA13, and HSPA14 expressions were inversely associated with the overall survival rate of patients, tumor grade, and cancer stage. A PPI regulatory network was constructed using the 14 HSP70s proteins with HSPA5 and HSPA8 at the network center. Univariate and multivariate analyses showed that HSPA4 and HSPA14 could be independent risk factors for the prognosis of hepatocellular carcinoma patients. Cell experiments have also confirmed that reducing HSPA4 and HSPA14 expressions can inhibit the invasion, metastasis, and proliferation of hepatocellular carcinoma cells.

Conclusions: Therefore, the HSP70s significantly influence the occurrence and development of hepatocellular carcinoma. For instance, HSPA4 and HSPA14 can be novel therapeutic targets and prognostic biomarkers for hepatocellular carcinoma.
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http://dx.doi.org/10.1186/s12935-021-01987-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165812PMC
May 2021

Au Nanoparticles Supported on Iron-Based Oxides for Soot Oxidation: Physicochemical Properties Before and After the Reaction.

ACS Omega 2021 May 26;6(17):11510-11518. Epub 2021 Apr 26.

Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Jinzhai Road, Hefei 230026, People's Republic of China.

The catalytic performance of Au nanoparticles (NPs) supported on different transition-metal oxides for soot oxidation was studied in this paper. The changes in the morphology, phase structure, and physicochemical properties of Au-supported iron-based oxides before and after the reaction with soot particles were observed by high-resolution transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and temperature-programed reduction. It was found that the catalytic activity of FeO, FeO, CoO, and NiO for soot oxidation was significantly improved after loading Au NPs. Especially, under the action of Au/FeO and Au/FeO, the oxidation of soot was close to 20% below 420 °C, and their values were 73 and 50 °C, respectively. When Au/FeO and Au/FeO reacted with soot, the size of the catalysts increased, and the active oxygen and Fe 2p components decreased. Au promoted the reduction of iron ions to a lower temperature, which was beneficial to improving the oxidation performance of iron-based oxides.
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http://dx.doi.org/10.1021/acsomega.1c00619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154002PMC
May 2021

Diagnostic Value of Combined Intravoxel Incoherent Motion Diffusion-Weighted Magnetic Resonance Imaging with Diffusion Tensor Imaging in Predicting Parametrial Infiltration in Cervical Cancer.

Contrast Media Mol Imaging 2021 11;2021:6651070. Epub 2021 May 11.

Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230031, China.

Objective: This study sought to determine the diagnostic value of combined intravoxel incoherent motion (IVIM) diffusion-weighted magnetic resonance imaging (MRI) with diffusion tensor imaging (DTI) in predicting parametrial infiltration (PMI) in patients with cervical cancer.

Materials And Methods: We enrolled 65 patients with cervical cancer confirmed by radical hysterectomy (25 PMI-negative and 40 PMI-positive) who underwent IVIM and DTI pretreatment. The parameters of IVIM (ADC, D, D  , and f) and DTI (average diffusion coefficient (DCavg) and fractional anisotropy (FA)) were recorded by two observers. All parameter differences were tested, and the receiver operating characteristic (ROC) curves were generated to estimate the diagnostic performance of significant metrics and their combinations.

Results: Compared to the PMI-negative group, the PMI-positive group had significantly lower D (0.632 ± 0.017 0.773 ± 0.024, < 0.001) and lower FA (0.073 ± 0.002 0.085 ± 0.003, =0.003). The area under the ROC curve (AUC) of D and FA was 0.801 and 0.726, respectively, and the combination of D and FA improved the AUC to 0.931, with a sensitivity and specificity of 80.0% and 97.5%, respectively.

Conclusion: D and FA values could be used to help diagnose PMI in patients with cervical cancer. The combination of IVIM and DTI was more valuable than either option alone.
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http://dx.doi.org/10.1155/2021/6651070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131167PMC
May 2021

Selective Imaging of HClO in the Liver Tissue In Vivo Using a Near-infrared Hepatocyte-specific Fluorescent Probe.

Chem Asian J 2021 Jul 7;16(14):1967-1972. Epub 2021 Jun 7.

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Wusi Dong Road 18, Baoding, 071002, P. R. China.

Liver injury is typified by an inflammatory response. Hypochlorous acid (HClO), an important endogenous reactive oxygen species, is regarded as a biomarker associated with liver injury. Near-infrared (NIR) fluorescent probes with the advantage of deep tissue penetrating and low auto-fluorescence interference are more suitable for bioimaging in vivo. Thus, in this work, we designed and synthesized a novel NIR hepatocyte-specific fluorescent probe named NHF. The probe NHF showed fast response (<3 s), large spectral variation, and good selectivity to trace HClO in buffer solution. By employing N-acetylgalactosamine (GalNAc) as the targeting ligand, probe NHF can be actively delivered to the liver tissue of zebrafish and mice. It is important that probe NHF is the first NIR hepatocyte-specific fluorescent probe, which successfully visualized the up-regulation of endogenous HClO in the oxygen-glucose deprivation/reperfusion (OGD/R) model HepG2 cells and dynamically monitored APAP-induced endogenous HClO in the liver tissue of zebrafish and mice.
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http://dx.doi.org/10.1002/asia.202100476DOI Listing
July 2021

NFKB2 inhibits NRG1 transcription to affect nucleus pulposus cell degeneration and inflammation in intervertebral disc degeneration.

Mech Ageing Dev 2021 07 21;197:111511. Epub 2021 May 21.

Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. Electronic address:

Extracellular matrix degradation, reactive oxygen species (ROS) generation, and inflammation in nucleus pulposus (NP) cells contribute to the progression of intervertebral disc degeneration (IDD). NRGs (Neuregulins) play a vital role in the development of the nervous system. In the present study, we found that NRG1 was downregulated within degenerative intervertebral disc and NP tissues, according to both bioinformatics and experimental analyses. Within IL-1β-stimulated NP cells, we observed degenerative and inflammatory changes, including inhibited cell viability, promoted cell apoptosis and ROS accumulation, reduced collagen II and aggrecan proteins, elevated MMP-3/13 and ADAMTS-4/5 proteins, and upregulated IL-6 and TNF-α mRNA levels. Within IL-1β-stimulated NP cells, NRG1 expression was also downregulated. NRG1 overexpression attenuated, whereas NRG1 silencing aggravated IL-1β-induced degenerative and inflammatory changes. Moreover, NRG1 regulated ErbB2/3 activation, contributing to the NRG1 protective function in NP cells. NFKB2 directly targeted the promoter region of NRG1 and inhibited NRG1 expression. In IL-1β-stimulated NP cells, silencing NFKB2 attenuated, whereas silencing NRG1 aggravated the degenerative changes and inflammation; the effects of NFKB2 silencing were significantly reversed by NRG1 silencing. In conclusion, NRG1 expression is downregulated within degenerative NP tissue samples and IL-1β-stimulated NP cells. NRG1 might protect against IL-1β-induced degenerative changes and inflammation. The upregulated NFKB2 might be the reason of NRG1 downregulation in degenerative NP tissues.
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http://dx.doi.org/10.1016/j.mad.2021.111511DOI Listing
July 2021

Recent Development of Oxygen Evolution Electrocatalysts in Acidic Environment.

Adv Mater 2021 May 25;33(20):e2006328. Epub 2021 Mar 25.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

The proton exchange membrane (PEM) water electrolysis is one of the most promising hydrogen production techniques. The oxygen evolution reaction (OER) occurring at the anode dominates the overall efficiency. Developing active and robust electrocatalysts for OER in acid is a longstanding challenge for PEM water electrolyzers. Most catalysts show unsatisfied stability under strong acidic and oxidative conditions. Such a stability challenge also leads to difficulties for a better understanding of mechanisms. This review aims to provide the current progress on understanding of OER mechanisms in acid, analyze the promising strategies to enhance both activity and stability, and summarize the state-of-the-art catalysts for OER in acid. First, the prevailing OER mechanisms are reviewed to establish the physicochemical structure-activity relationships for guiding the design of highly efficient OER electrocatalysts in acid with stable performance. The reported approaches to improve the activity, from macroview to microview, are then discussed. To analyze the problem of instability, the key factors affecting catalyst stability are summarized and the surface reconstruction is discussed. Various noble-metal-based OER catalysts and the current progress of non-noble-metal-based catalysts are reviewed. Finally, the challenges and perspectives for the development of active and robust OER catalysts in acid are discussed.
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http://dx.doi.org/10.1002/adma.202006328DOI Listing
May 2021

Preoperative Prediction of Cervical Nodal Metastasis in Papillary Thyroid Carcinoma: Value of Quantitative Dual-Energy CT Parameters and Qualitative Morphologic Features.

AJR Am J Roentgenol 2021 05 24;216(5):1335-1343. Epub 2021 Mar 24.

Department of Radiology, Division of Life Sciences and Medicine, The First Affiliated Hospital of the University of Science and Technology of China, Huanhu Rd 107, Hefei, Anhui 230031, PRC. Address correspondence to J. Dong

The purpose of our study was to assess the value of combining quantitative dual-energy CT (DECT) parameters with qualitative morphologic parameters for the preoperative prediction of cervical nodal metastasis from papillary thyroid carcinoma (PTC). Thirty-five patients with pathologically proven PTC underwent single-phase contrast-enhanced DECT before thyroidectomy and cervical lymphadenectomy. Analyses of quantitative DECT parameters and qualitative morphologic features of metastatic and benign lymph nodes (LNs) were independently performed. The diagnostic performances of using only quantitative parameters, only morphologic features, and their combination for predicting cervical nodal metastasis were statistically calculated with ROC curves and logistic regression models. A total of 206 LNs, 80 metastatic and 126 benign, were included. The best single performer in DECT was the normalized iodine concentration in the venous phase, which had low sensitivity (62.5%) but high specificity (85.7%), for diagnosing metastatic cervical LNs. On the other hand, the best single performer in qualitative morphologic parameters was using the criterion of shortest diameter of greater than 5 mm, which had low specificity (69.8%) but high sensitivity (86.3%). Combining these two parameters improved the AUC, sensitivity, and specificity to 0.846, 86.3%, and 72.2%, respectively. The combination of multiple quantitative DECT parameters and all morphologic data further improved AUC, sensitivity, and specificity to 0.878, 87.5%, and 73.8%, respectively, which was significant compared with the use of any single parameter. The combination of quantitative DECT parameters with morphologic data improves performance in the preoperative diagnosis of metastatic cervical LNs in patients with PTC.
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http://dx.doi.org/10.2214/AJR.20.23516DOI Listing
May 2021

Hsa_circ_001659 serves as a novel diagnostic and prognostic biomarker for colorectal cancer.

Biochem Biophys Res Commun 2021 04 13;551:100-106. Epub 2021 Mar 13.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 200240, China. Electronic address:

Colorectal cancer (CRC) is prevalent worldwide and novel diagnostic and prognostic biomarkers are needed to improve precision medicine. Circular RNAs (circRNAs) are currently being considered as emerging tumor biomarkers. Herein, we aimed to explore the possible clinical application of circRNAs in the early diagnosis and prognostic prediction of CRC. First, candidate circRNA was selected by integrating analysis of Gene Expression Omnibus (GEO) database using GEO2R program. ROC curve analysis demonstrated the predictive values and likelihood ratios of circ_001659 were satisfactory for the diagnosis of CRC, including patients in early-stage disease or patients with carcinoembryonic antigen (CEA)-negative status. Moreover, serum circ_001659 may be a novel biomarker in the assessment of successful treatment and remission of cancer tracking. We further investigated the oncogenic role of circ_001659. In vivo and in vitro experiments indicated that circ_001659 could promote CRC cell invasion and migration. Mechanistically, circ_001659 was localized in the nucleus, recruited the RBBP5 to Vimentin promoter and increased H3K4 trimethylation level on the Vimentin promoter region, which epigenetically activated Vimentin transcription. Our findings demonstrate that circ_001659 could be a useful serum biomarker for CRC diagnosis and prognosis. Targeting circ_001659 and its pathway may be meaningful for treating patients with CRC.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.121DOI Listing
April 2021

A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.

Theor Appl Genet 2021 May 16;134(5):1377-1386. Epub 2021 Mar 16.

National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Key Message: A mitochondria-localized pentatricopeptide repeat protein was identified by positional cloning and transferred into the hau CMS line, where it successfully restored fertility Cytoplasmic male sterility (CMS) is a maternally inherited trait that can be controlled by restorer-of-fertility (Rf) genes present in the nucleus. The hau CMS was identified as a new form of CMS associated with the mitochondrial transcript orf288; however, a lack of a restorer gene has limited its utilization in Brassica crops. Here, the combination of Brassica 60 K array with bulk segregant analysis and map-based cloning was used to delimit the Rfh locus to an 82.2-kb region on chromosome A09. A candidate gene encoding a mitochondria-localized pentatricopeptide repeat (PPR) protein was identified and transferred into the hau CMS line, where it successfully restored the fertility of the hau CMS plants. Furthermore, the expression analysis showed that Rfh was highly expressed in the flower buds, and the sequence analysis results implied that functional divergence between RFH and rfh could be due to 59 amino acid residue differences in the deduced protein sequences. In addition, a co-separated molecular marker was developed based on the divergent sequences between the dominant and recessive alleles. These results will help enable the heterosis of Brassica crops in the future.
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http://dx.doi.org/10.1007/s00122-021-03777-3DOI Listing
May 2021

Curdione Induces Antiproliferation Effect on Human Uterine Leiomyosarcoma Targeting IDO1.

Front Oncol 2021 26;11:637024. Epub 2021 Feb 26.

School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Objectives: Curdione is one of the active ingredients of a traditional Chinese herbal medicine- and established anti-tumor effects. Uterine leiomyosarcoma (uLMS) is a rare gynecological malignancy, with no standard therapeutic regimen at present. The aim of this study was to explore the potential anti-tumor impact of curdione in uLMS and elucidate the underlying mechanisms.

Methods: functional assays were performed in the SK-UT-1 and SK-LMS-1 cell lines. The model of uLMS was established by subcutaneously injecting SK-UT-1 cells, and the tumor-bearing mice were intraperitoneally injected with curdione. Tumor weight and volume were measured at specific time points. The biosafety was evaluated by monitoring changes of body weight and the histopathology in the liver and kidney. The expression levels of relevant proteins were analyzed by western blotting and immunohistochemistry.

Results: Curdione decreased the viability and proliferation of uLMS cells in a concentration and time-dependent manner. In addition, the curdione-treated cells exhibited significantly higher rates of apoptosis and autophagic death. Curdione also decreased the tumor weight and volume in the SK-UT-1 xenograft model compared to the untreated control without affecting the body bodyweight or pathological injury of liver and kidney tissues. At the molecular level, the anti-tumor effects of curdione were mediated by indoleamine-2, 3-dioxygenase-1 (IDO1).

Conclusion: Curdione exhibited an anti-uLMS effect and ; the underlying mechanism involved in IDO1 mediate apoptosis, autophagy, and G2/M phase arrest.
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http://dx.doi.org/10.3389/fonc.2021.637024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953905PMC
February 2021

A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.

Exp Eye Res 2021 04 27;205:108509. Epub 2021 Feb 27.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China; Eye Hospital of Shandong First Medical University, Jinan, Shandong Province, China. Electronic address:

Mooren's ulcer (MU) is a refractory autoimmune corneal ulcer with a high recurrence rate. So far, its molecular profiles and pathomechanisms remain largely unknown. Therefore, we aim to characterize the protein profiles of MU specimens by data-independent-acquisition (DIA) mass spectrometry (MS), and to define the functions of differentially-expressed proteins (DEPs). Through LC-MS/MS, 550 DEPs were identified between MU biopsies and age-matched controls (Ctrl). KEGG analysis revealed that the significantly enriched pathways of the up-regulated proteins mainly covered lysosomes, antigen processing and presentation, and phagosomes. We subsequently validated the expressions of the selected candidates using parallel-reaction-monitoring (PRM)-based MS and immunohistochemistry (IHC), including cathepsins, TIMP3, MMP-10, MYOC, PIGR, CD74, CAT, SOD2, and SOD3. Moreover, immunoglobulin (Ig) components and B lymphocytes associated proteins MZB1, HSPA5, and LAP3 in MU were significantly increased and validated by PRM-based MS and IHC. The remarkable enrichment of neutrophil extracellular traps (NETs) components in MU samples was also identified and determined. The up-regulated Ig components and NETs components suggested that B lymphocytes and neutrophils participated in the immunopathology of MU. Importantly, we also identified and validated much more expression of peptidyl arginine deiminase 4 (PADI4) in MU samples. The double-immunofluorescence staining showed the co-localization of citrulline residues with MPO, NE, and IgG in MU samples. These results indicated the presences of PADI4-mediated citrullination modification and anti-citrullinated protein antibodies (ACPAs) in MU samples. Our findings, for the first time, provide a global proteomic signature of MU, which may open a new avenue towards disease pathology and therapeutics.
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http://dx.doi.org/10.1016/j.exer.2021.108509DOI Listing
April 2021

The Key Regulator of Necroptosis, RIP1 Kinase, Contributes to the Formation of Astrogliosis and Glial Scar in Ischemic Stroke.

Transl Stroke Res 2021 Feb 24. Epub 2021 Feb 24.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Ren-Ai Road, Suzhou, 215123, Jiangsu, China.

Necroptosis initiation relies on the receptor-interacting protein 1 kinase (RIP1K). We recently reported that genetic and pharmacological inhibition of RIP1K produces protection against ischemic stroke-induced astrocytic injury. However, the role of RIP1K in ischemic stroke-induced formation of astrogliosis and glial scar remains unknown. Here, in a transient middle cerebral artery occlusion (tMCAO) rat model and an oxygen and glucose deprivation and reoxygenation (OGD/Re)-induced astrocytic injury model, we show that RIP1K was significantly elevated in the reactive astrocytes. Knockdown of RIP1K or delayed administration of RIP1K inhibitor Nec-1 down-regulated the glial scar markers, improved ischemic stroke-induced necrotic morphology and neurologic deficits, and reduced the volume of brain atrophy. Moreover, knockdown of RIP1K attenuated astrocytic cell death and proliferation and promoted neuronal axonal generation in a neuron and astrocyte co-culture system. Both vascular endothelial growth factor D (VEGF-D) and its receptor VEGFR-3 were elevated in the reactive astrocytes; simultaneously, VEGF-D was increased in the medium of astrocytes exposed to OGD/Re. Knockdown of RIP1K down-regulated VEGF-D gene and protein levels in the reactive astrocytes. Treatment with 400 ng/ml recombinant VEGF-D induced the formation of glial scar; conversely, the inhibitor of VEGFR-3 suppressed OGD/Re-induced glial scar formation. RIP3K and MLKL may be involved in glial scar formation. Taken together, these results suggest that RIP1K participates in the formation of astrogliosis and glial scar via impairment of normal astrocyte responses and enhancing the astrocytic VEGF-D/VEGFR-3 signaling pathways. Inhibition of RIP1K promotes the brain functional recovery partially via suppressing the formation of astrogliosis and glial scar.
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http://dx.doi.org/10.1007/s12975-021-00888-3DOI Listing
February 2021

Targeting Toll-Like Receptors in Sepsis: From Bench to Clinical Trials.

Antioxid Redox Signal 2021 Apr 7. Epub 2021 Apr 7.

Translational Research Program, Department of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Sepsis is a critical clinical syndrome with life-threatening organ dysfunction induced by a dysregulated host response to infection. Despite decades of intensive research, sepsis remains a leading cause of in-hospital mortality with few specific treatments. Toll-like receptors (TLRs) are a part of the innate immune system and play an important role in host defense against invading pathogens such as bacteria, virus, and fungi. Using a combination of genetically modified animal models and pharmacological agents, numerous preclinical studies during the past two decades have demonstrated that dysregulated TLR signaling may contribute to sepsis pathogenesis. However, many clinical trials targeting inflammation and innate immunity such as TLR4 have yielded mixed results. Here we review various TLRs and the specific molecules these TLRs sense-both the pathogen-associated and host-derived stress molecules, and their converging signaling pathways. We critically analyze preclinical investigations into the role of TLRs in animal sepsis, the complexity of targeting TLRs for sepsis intervention, and the disappointing clinical trials of the TLR4 antagonist eritoran. Future sepsis treatments will depend on better understanding the complex biological mechanisms of sepsis pathogenesis, the high heterogeneity of septic humans as defined by clinical presentations and unique immunological biomarkers, and improved stratifications for targeted interventions.
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http://dx.doi.org/10.1089/ars.2021.0005DOI Listing
April 2021

Spatial-Temporal Characteristics of Illegal Land Use and Its Driving Factors in China from 2004 to 2017.

Int J Environ Res Public Health 2021 02 2;18(3). Epub 2021 Feb 2.

School of Politics, Law and Public Administration, Hubei University, Wuhan 430000, China.

The scientific analysis of spatial-temporal differentiation characteristics and driving factors of illegal land use is of great significance for the formulation and optimization of policies to control the emergence of illegal land use. This paper establishes two variable systems of illegal land use and its driving factors, defined the multidimensional characteristic variables of illegal land use and analyzes the relationships among them by the Pearson's correlation coefficient; In addition, the spatial-temporal characteristics of each variable of illegal land use from 2004 to 2017 are described by the spatial autocorrelation analysis; Finally, based on the geographical detectors, the influence direction and degree of the factors of economic structure, social structure and land market behavior on the characteristics of different illegal land use are studied. The results show that the spatial agglomeration of different characteristics of illegal land use had been weakening from 2004 to 2017, but the rate of weakening was different, and L-L agglomeration changed between Xinjiang and other central-western provinces, H-H agglomeration changed in the coastal regions of the central-eastern of China, the level and ability of the central government and local governments to govern illegal land use is constantly improving on the whole; the compositional factors of economic development structure, social development structure, and land market behavior of driving factors had different influence in the degree, the location or the direction of different characteristics of illegal land use. According to the spatial-temporal characteristics and the differences of driving factors, managers can formulate differentiated illegal land use control policies, which will help to control the occurrence of illegal land use and help the settlement of illegal land use cases, and ultimately achieve sustainable development.
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http://dx.doi.org/10.3390/ijerph18031336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908213PMC
February 2021

Hypobaria Exposure Worsens Cardiac Function and Endothelial Injury in AN Animal Model of Polytrauma: Implications for Aeromedical Evacuation.

Shock 2021 10;56(4):601-610

Translational Research Program, Department of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, Maryland.

Background: Aeromedical evacuation can expose traumatically injured patients to low pressure (hypobaria) and hypoxia. Here, we sought to assess the impact of hypobaria on inflammation, organ injury, and mortality in a mouse model of polytrauma.

Methods: Eight to 12-week-old male C57BL/6J mice were subjected to sham or polytrauma consisting of bowel ischemia by superior mesenteric artery occlusion, hindlimb muscle crush, and tibia fracture. Two hours after injury, animals were randomized to undergo either 6 h of hypobaria or sea-level, room air conditions. At 8 or 24 h after injury, transthoracic echocardiography was performed. Acute kidney injury (AKI) biomarkers were measured by qRT-PCR. Plasma cytokine and endothelial injury markers were determined by enzyme-linked immunosorbent assay.

Results: Eight hours after traumatic injury, mice exhibited a marked increase in plasma IL-6 (57 pg/mL vs. 1,216 pg/mL), AKI with increased Ngal and Kim-1, and endothelial injury as evidenced by significantly increased plasma hyaluronic acid (96 ng/mL vs.199 ng/mL), thrombomodulin (23.2 ng/mL vs. 58.9 ng/mL), syndecan-1 (0.99 ng/mL vs. 4.34 ng/mL), and E-selectin (38.6 ng/mL vs. 62.7 ng/mL). The trauma mice also developed cardiac dysfunction with decreased cardiac output and stroke volume at 8 h postinjury. Hypobaric exposure after polytrauma led to decreased ejection fraction (81.0% vs. 74.2%, P < 0.01) and increased plasma hyaluronic acid (199 ng/mL vs. 260 ng/mL, P < 0.05), thrombomodulin (58.9 ng/mL vs. 75.4 ng/mL, P < 0.05), and syndecan-1 (4.34 ng/mL vs. 8.33 ng/mL, P < 0.001) at 8 h postinjury.

Conclusions: Hypobaria exposure appeared to worsen cardiac dysfunction and endothelial injury following polytrauma and thus may represent a physiological "second hit" following traumatic injury.
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http://dx.doi.org/10.1097/SHK.0000000000001716DOI Listing
October 2021

Spatiotemporal spread pattern of the COVID-19 cases in China.

PLoS One 2020 31;15(12):e0244351. Epub 2020 Dec 31.

College of Surveying and Geo-Informatics, Tongji University, Shanghai, China.

The COVID-19 pandemic is currently spreading widely around the world, causing huge threats to public safety and global society. This study analyzes the spatiotemporal pattern of the COVID-19 pandemic in China, reveals China's epicenters of the pandemic through spatial clustering, and delineates the substantial effect of distance to Wuhan on the pandemic spread. The results show that the daily new COVID-19 cases mostly occurred in and around Wuhan before March 6, and then moved to the Grand Bay Area (Shenzhen, Hong Kong and Macau). The total COVID-19 cases in China were mainly distributed in the east of the Huhuanyong Line, where the epicenters accounted for more than 60% of the country's total in/on 24 January and 7 February, half in/on 31 January, and more than 70% from 14 February. The total cases finally stabilized at approximately 84,000, and the inflection point for Wuhan was on 14 February, one week later than those of Hubei (outside Wuhan) and China (outside Hubei). The generalized additive model-based analysis shows that population density and distance to provincial cities were significantly associated with the total number of the cases, while distances to prefecture cities and intercity traffic stations, and population inflow from Wuhan after 24 January, had no strong relationships with the total number of cases. The results and findings should provide valuable insights for understanding the changes in the COVID-19 transmission as well as implications for controlling the global COVID-19 pandemic spread.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244351PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775067PMC
January 2021

Prognostic Value of Androgen Receptor Splice Variant 7 in the Treatment of Metastatic Castration-Resistant Prostate Cancer: A Systematic Review and Meta-Analysis.

Front Oncol 2020 30;10:562504. Epub 2020 Nov 30.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The prognostic value of androgen receptor splice variant 7 (AR-V7) for the treatment response of metastatic castration-resistant prostate cancer (mCRPC) remains unclear. In this study, we aimed to synthesize relevant studies that assessed the prognostic value of AR-V7 status for the treatment response of mCRPC patients treated with androgen receptor signalling inhibitors (ARSis) and chemotherapy.

Methods: We searched the PubMed, Embase, and MEDLINE databases by using the keywords and to identify relevant studies published before 25 September 2019. The main outcomes were prostate-specific antigen (PSA) response, progression-free survival (PFS), and overall survival (OS). Pooled odds ratios (ORs) and hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated using a random effects model. The quality of the included studies was assessed using the Newcastle-Ottawa Quality Assessment Scale.

Results: A total of 1,545 patients from 21 studies were included. For the mCRPC patients treated with ARSis, AR-V7-positive patients had a lower PSA response rate (OR 6.01, 95% CI 2.88-12.51; < 0.001), shorter PFS (HR 2.56, 95% CI 1.80-3.64;  < 0.001) and shorter OS (HR 4.28, 95% CI 2.92-6.27; < 0.001) than AR-V7-negative patients. Although AR-V7-positive patients treated with chemotherapy also had a lower PSA response rate (OR 2.23, 95% CI 1.38-3.62; = 0.001) and shorter OS than AR-V7-negative patients (HR 1.60, 95% CI 1.02-2.53; = 0.043), there was no significant difference in PFS (HR 1.05, 95% CI 0.74-1.49; = 0.796) between these groups. Furthermore, AR-V7-positive patients receiving ARSis had a shorter median OS than those receiving chemotherapy (HR 3.50, 95% CI 1.98-6.20; < 0.001); There was no significant difference among AR-V7-negative patients (HR 1.30, 95% CI 0.64-2.62;  = 0.47).

Conclusions: AR-V7 is a potential biomarker of treatment resistance in mCRPC patients. AR-V7-positive mCRPC patients had poorer treatment outcomes than AR-V7-nagetive patients when treated with ARSis. AR-V7-positive patients have better outcomes when treated with taxane than ARSis. Furthermore, the ability of AR-V7 status to predict treatment outcomes varies from different detection methods. The detection of AR-V7 before treatment is important for the selection of treatment modalities for mCRPC patients.
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http://dx.doi.org/10.3389/fonc.2020.562504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735106PMC
November 2020
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