Publications by authors named "Chao Wang"

5,359 Publications

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High-throughput fluorescent screening of β-lactamase inhibitors to improve antibiotic treatment strategies for tuberculosis.

Biosens Bioelectron 2022 Aug 2;216:114606. Epub 2022 Aug 2.

Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China; School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China. Electronic address:

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), which is a major threat to global public health. Currently, β-lactam antibiotics are rarely used in the treatment of TB, since Mtb naturally expresses β-lactamase (Blac) which renders Mtb resistant to such antibiotics due to β-lactam cleavage. Fortunately, antibiotic resistance can be overcome when β-lactam antibiotics are combined with a Blac inhibitor. With the current research, a near-infrared fluorescent probe LXMB was developed for the real-time detection and imaging of endogenous Blac activity in Mtb. Furthermore, a high-throughput screening platform was established using LXMB to screen Blac inhibitors from herbal medicines. Guided by the visual bioassay, Tannic acid was isolated from Galla Chinensis as a potential Blac inhibitor and was further evaluated in combination with several β-lactam antibiotics which resulted in an enhanced inhibitory effect toward M. tuberculosis H37Ra. Finally, LXMB was used to label live M. tuberculosis H37Ra phagocytosed within macrophages. Consequently, LXMB was a useful fluorescent tool to explore the mechanism of drug resistance based on Blac and can assist in the development of new tuberculosis treatments.
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http://dx.doi.org/10.1016/j.bios.2022.114606DOI Listing
August 2022

Elevated expression of the membrane-anchored serine protease TMPRSS11E in NSCLC progression.

Carcinogenesis 2022 Aug 11. Epub 2022 Aug 11.

Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, Medicine School of Southeast University, Nanjing, 210009, China.

TMPRSS11E was found to be upregulated in human non-small cell lung cancer samples (NSCLC) and cell lines, and high expression was associated with poor survival of NSCLC patients. The results of in vitro and in vivo experiments showed that overexpressing TMPRSS11E resulted in A549 cell proliferation and migration promotion, while the TMPRSS11E S372A mutant with the mutated catalytic domain lost the promoting function. In addition, in mouse xenograft models, silencing TMPRSS11E expression inhibited the growth of 95D cell-derived tumors. To explore the mechanism of marked upregulation of TMPRSS11E in NSCLC cells, promoter analysis, EMSA and ChIP assays were performed. STAT3 was identified as the transcription factor responsible for TMPRSS11E transcription. Moreover, the purified recombinant TMPRSS11E catalytic domain exhibited enzymatic activity for the proteolytic cleavage of PAR2. Recombinant TMPRSS11E catalytic domain incubation further activated the PAR2-EGFR-STAT3 pathway. These findings established a mechanism of TMPRSS11E-PAR2-EGFR-STAT3 positive feedback, and the oncogenic role of TMPRSS11E as a PAR2 modulator in NSCLC was revealed.
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http://dx.doi.org/10.1093/carcin/bgac069DOI Listing
August 2022

Biomechanical evaluation of customized root implants in alveolar bone: a comparative study with traditional implants and natural teeth.

J Prosthodont 2022 Aug 11. Epub 2022 Aug 11.

Key Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing, China.

Purpose: To compare and evaluate density changes in alveolar bones and biomechanical responses including stress/strain distributions around customized root implants (CRIs), traditional implants, and natural teeth.

Materials And Methods: A three-dimensional finite element model of the maxillary dentition defect, CRI models, traditional restored implant models, and natural teeth with periodontal tissue models were established. The chewing load of the central incisor, the traditional implant, and the CRI was 100N, and the load direction was inclined by 11° in the sagittal plane. According to the bone remodeling numerical algorithm, the bone mineral density and distribution were calculated and predicted. In addition, animal experiments were performed to verify the feasibility of the implant design. The results of the simulation calculations were compared with animal experimental data in vivo to verify their validity.

Results: No significant differences in bone mineral density and stress/strain distribution were found between the CRI and traditional implant models. The animal experimental results (X-ray images and histological staining) were consistent with the numerical simulated results.

Conclusions: CRIs were more similar to traditional implants than to natural teeth in terms of biomechanical and biological evaluation. Considering the convenience of clinical application, this biomechanical evaluation provides basic theoretical support for further applications of CRI. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jopr.13590DOI Listing
August 2022

Author Correction: Integrated characterization of SARS-CoV-2 genome, microbiome, antibiotic resistance and host response from single throat swabs.

Cell Discov 2022 Aug 10;8(1):78. Epub 2022 Aug 10.

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.

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http://dx.doi.org/10.1038/s41421-022-00448-5DOI Listing
August 2022

Genetically Engineered Hematopoietic Stem Cells Deliver TGF-β Inhibitor to Enhance Bone Metastases Immunotherapy.

Adv Sci (Weinh) 2022 Aug 10:e2201451. Epub 2022 Aug 10.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 199 Ren'ai Road, Suzhou, Jiangsu, 215123, China.

Owing to the immune microenvironment of bones and low selectivity of the drug, patients with bone metastases often respond poorly to immunotherapy. In this study, programmed cell death protein 1 (PD1)-expressing hematopoietic stem cells (HSCs) are genetically engineered for bone-targeted delivery of the transforming growth factor beta (TGF-β) small-molecule inhibitor SB-505124 ([email protected]). Intriguingly, compared to anti-PD-L1 monoclonal antibodies, as "living drugs", HSCs-PD-1 not only show great targeting ability to the bone marrow, but are also able to reduplicate themselves within the bone marrow niche and continuously express PD-1 molecules. The SB released from HSCs-PD-1 competitively bound to TGF-β receptors on CD4 T cells and facilitate CD4 T cell differentiation to helper T (T )1 and T 2 cells, thereby reprogramming the local immunosuppressive milieu of the bone marrow. Additionally, HSCs-PD-1 can block programmed death-ligand 1 on tumor and myeloid cells, resulting in reinvigorated anti-tumor immunity of T cells. In conclusion, in the present study, an alternative cell engineering strategy is delineated for immune checkpoint blockade therapy, to target bone metastasis using HSCs as a platform, which shows great promise in the treatment of bone metastases.
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http://dx.doi.org/10.1002/advs.202201451DOI Listing
August 2022

Prediabetes and osteoporotic fracture risk: A meta-analysis of prospective cohort studies.

Diabetes Metab Res Rev 2022 Aug 10:e3568. Epub 2022 Aug 10.

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Translational Medicine Center, Chinese Academy of Medical Sciences, ;, Beijing, 100730, China.

Background: Diabetes confers an increased risk of fracture. However, whether prediabetes is also a risk factor of osteoporotic fracture has not been comprehensively examined. We performed a meta-analysis to evaluate the relationship between prediabetes and osteoporotic fracture risk.

Methods: This meta-analysis included relevant prospective cohort studies from Medline, Embase, and Web of Science databases. A random-effect model after incorporation of the intra-study heterogeneity was selected to pool the results. Subgroup analyses were applied to evaluate the influences of study characteristics on relationship between prediabetes and osteoporotic fracture risk.

Results: Eight studies including 33136 community dwelling adult patients were included, and 7429 (22.4%) patients were prediabetic. Prediabetes was not independently associated with a higher risk of osteoporotic fracture compared with normoglycemia (adjusted risk ratio [RR]: 1.03, 95% confidence interval [CI]: 0.88-1.21, P = 0.69, I = 42%). Sensitivity limited to the elderly population showed consistent results (RR: 1.10, 95% CI: 0.91-1.24, P = 0.15, I = 0%). Subgroup analysis suggested that prediabetes defined by HbA1c (approximately 5.7-6.4%) was associated with a higher risk of osteoporotic fracture (RR: 1.24, 95% CI: 1.01-1.53, P = 0.04), but not that defined by impaired fasting glucose or impaired glucose tolerance (P = 0.60). Sex, follow-up duration, and adjustment of bone mineral density did not significantly affect the outcome.

Conclusions: Current evidence does not support that prediabetes is independently associated with osteoporotic fracture risk. Different definitions of prediabetes may affect the association between prediabetes and osteoporotic fracture risk. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/dmrr.3568DOI Listing
August 2022

Oxidative Damage to BV2 Cells by Trichloroacetic Acid: Protective Role of Boron the p53 Pathway.

Biomed Environ Sci 2022 Jul;35(7):657-662

China CDC Key Laboratory of Environment and Population Health.

This study aimed to investigate the neurotoxicity induced by trichloroacetic acid (TCA) and the possible protective mechanisms of boron (B). Mouse BV2 cells were treated with TCA (0, 0.39, 0.78, 1.56, 3.12, 6.25, or 12.5 mmol/L) and B (0, 7.8, 15.6, 31.25, 62.5, 125, 500, or 1,000 mmol/L) for 3 h and 24 h, respectively. Then, reactive oxygen species, and supernatant proinflammatory cytokine and protein levels were analyzed after 24 h of combined exposure. Beyond the dose-dependent decrease in the cellular viability, it clearly increased after B supplementation ( < 0.05). Moreover, B decreased oxidative damage, and significantly down-regulated IL-6 levels and up-regulated TNF-β production ( < 0.05). B also decreased apoptosis the p53 pathway. The present findings indicated that TCA may induce oxidative damage, whereas B mitigates these adverse effects by decreasing cell apoptosis.
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http://dx.doi.org/10.3967/bes2022.086DOI Listing
July 2022

Microbial transformation of capsaicin by several human intestinal fungi and their inhibitory effects against lysine-specific demethylase 1.

Phytochemistry 2022 Aug 6;202:113365. Epub 2022 Aug 6.

College of Pharmacy, Academy of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China. Electronic address:

Capsaicin widely exists in the Capsicum genus (e.g., hot peppers) and is commonly used as a food additive or medicinal material. In this work, microbial transformation of capsaicin was performed based on the three cultivated human intestinal fungi. Fourteen metabolites were obtained, and their chemical structures were elucidated by spectroscopic data analysis, including 13 compounds with undescribed structures. Hydroxylation, lactylation, succinylation, citric acylation, and acetylation were observed for these microbial metabolites derived from capsaicin, which indicated diverse catalytic characteristics of human intestinal fungi. In an in vitro bioassay, four metabolites and capsaicin inhibited the activity of lysine-specific demethylase 1 (LSD1) with a more than 70% inhibitory rate at 10 μM. In particular, 9,5'-dihydroxycapsaicin displayed the strongest inhibitory effect with an IC of 1.52 μM. Therefore, capsaicin analogs displayed potential application as LSD1 inhibitors against the invasion and migration of cancer cells.
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http://dx.doi.org/10.1016/j.phytochem.2022.113365DOI Listing
August 2022

Effects and potential mechanism of Ca/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle.

Int J Mol Med 2022 Oct 5;50(4). Epub 2022 Aug 5.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China.

Adequate regulation of the speed of follicular development has been reported to prolong the reproductive life of the ovary. The aim of the present study was to assess the potential effects and mechanism of the Ca/calmodulin‑dependent protein kinase II (CaMKII) pathway on the development of ovarian follicle. In the present study, the expression of CaMKII was measured in the ovary of mice at different developmental stages by immunofluorescence, confirming that CaMKII has a role in follicular development. Subsequently, the 17.5 days post‑coitus (dpc) embryonic ovaries were collected and cultured with KN93 for 4 days in vitro. It was revealed that KN93 inhibited the development of follicles, where it reduced the expression levels of oocyte and granulosa cell markers DEAD‑box helicase 4 (DDX4) and forkhead box L2 (FOXL2). These results suggested that KN93 could delay follicular development. Proteomics technology was then used to find that 262 proteins of KN93 treated 17.5 dpc embryonic ovaries were significantly altered after in vitro culture. Bioinformatics analysis was used to analyze these altered proteins. In total, four important Kyoto Encyclopedia of Genes and Genome pathways, namely steroid biosynthesis, p53 signaling pathway and retinol metabolism and metabolic pathways, were particularly enriched. Further analysis revealed that the upregulated proteins NADP‑dependent steroid dehydrogenase‑like (Nsdhl), lanosterol synthase (Lss), farnesyl‑diphosphate farnesyltransferase 1 (Fdft1), cytochrome P450 family 51 family A member 1 (Cyp51a1), hydroxymethylglutaryl‑CoA synthase 1 (Hmgcs1), fatty acid synthase (Fasn) and dimethylallyltranstransferase (Fdps) were directly interacting with each other in the four enriched pathways. In summary, the potential mechanism of KN93 in slowing down follicular development most likely lies in its inhibitory effects on CaMKII, which upregulated the expression of Nsdhl, Lss, Fdft1, Cyp51a1, Hmgcs1, Fasn and Fdps. This downregulated the expression of oocyte and granulosa cell markers DDX4 and FOXL2 in the follicles, thereby delaying follicular development. Overall, these results provide novel insight into the potential mechanism by which KN93 and CaMKII can delay follicular development.
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http://dx.doi.org/10.3892/ijmm.2022.5177DOI Listing
October 2022

Nitrogen Disturbance Awakening the Intrinsic Activity of Nickel Phosphide for Boosted Hydrogen Evolution Reaction.

ChemSusChem 2022 Aug 4:e202201346. Epub 2022 Aug 4.

College of Chemistry and Materials Engineering, Zhejiang A&F University, Hangzhou, Zhejiang Province, 311300, P. R. China.

Invited for this month's cover is the group of Qingfeng Sun at the Zhejiang A&F University. The image shows that the N-doped Ni P exhibits excellent electrocatalytic activity for hydrogen evolution reaction (HER). The Research Article itself is available at 10.1002/cssc.202200072.
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http://dx.doi.org/10.1002/cssc.202201346DOI Listing
August 2022

Visual Identification of a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe.

Anal Chem 2022 Aug 3. Epub 2022 Aug 3.

Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China.

Lipase found in the gut microbiota participates in the digestion and absorption of dietary fats. As such, the gut microbiota is involved in the regulation of the host metabolism, affecting the levels of lipids and free fatty acids, ultimately resulting in obesity. In this study, an enzymatic activatable near-infrared fluorescent probe, , was developed for visually sensing endogenous lipase from gut microbes. Using , a cultivated intestinal yeast strain was rapidly identified from human feces that exhibited high lipase expression and was identified as Y2. We then determined that the colonization of the gut of mice with Y2 increased lipase activity in the digestive tract and promoted obesity and hyperlipidemia when the mice were fed high fat diets. Above all, the present research resulted in a fluorescence visualization tool for the functional investigation of gut microbiota associated with obesity and disorders of lipid metabolism.
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http://dx.doi.org/10.1021/acs.analchem.2c01691DOI Listing
August 2022

Development and external validation of a novel nomogram to predict prostate cancer in biopsy-naïve patients with PSA <10 ng/ml and PI-RADS v2.1 = 3 lesions.

Cancer Med 2022 Aug 3. Epub 2022 Aug 3.

Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Objective: To develop and externally validate a novel nomogram in biopsy-naïve patients with prostate-specific antigen (PSA) <10 ng/ml and PI-RADS v2.1 = 3 lesions.

Methods: We retrospectively collected 307 men that underwent initial biopsy from October 2015 to January 2022 in Cohort 1 (The First Affiliated Hospital of Soochow University). External cohort (Cohort 2, Kunshan Hospital) included 109 men that met our criteria from July 2016 to June 2021. By Slicer-3D Software, the volume of all lesions was divided into two subgroups (PI-RADS v2.1 = 3a and 3b). Logistic regression analysis was performed to screen for variables and construct nomogram by analyzing clinical data from Cohort 1. Receiver operating characteristics curve analysis, calibration plot and decision curve analysis (DCA) were plotted to validate the nomogram in external cohort.

Results: A total of 70 (22.8%) patients was diagnosed with prostate cancer in Institution 1. Among them, 34 (11.1%) had clinically significant prostate cancer (csPCa). Age, prostate-specific antigen density, digital rectal examination, PI-RADS v2.1 = 3 subgroups (3a and 3b) and apparent diffusion coefficient (ADC, <750 mm /s) were predictive factors for prostate cancer (PCa) and csPCa. High area under the curve of the nomogram was found in Cohort 1 and Cohort 2 for PCa (0.857 vs. 0.850) and for csPCa (0.896 vs. 0.893). Calibration curves showed excellent agreement between the predicted probability and actual risk for the models in internal and external validation. The DCA demonstrated net benefit of our nomogram.

Conclusion: Until now, this is the first nomogram that predicts PCa and csPCa in biopsy-naïve patients with PSA <10 ng/ml and PI-RADS v2.1 = 3 lesions. Furthermore, PI-RADS v2.1 = 3 subgroups were considered to be an independent risk factor in our model. Our nomogram may assist urologists in biopsy decision making for these so-called "double gray zone" patients.
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http://dx.doi.org/10.1002/cam4.5100DOI Listing
August 2022

Prevalence of Metabolic Syndrome and Risk Factors Among Chinese Adults: Results from a Population-Based Study - Beijing, China, 2017-2018.

China CDC Wkly 2022 Jul;4(29):640-645

Institute of Reproductive and Child Health / Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Peking University, Beijing, China.

What Is Already Known About This Topic?: Metabolic syndrome (MetS) is one of the most easily available health indicative markers for cardiovascular diseases, and it has become a major public health problem worldwide due to increasing urbanization and aging populations. The prevalence of MetS increased dramatically in China, however, there are no records of MetS defined by the 2017 Chinese Diabetes Society for Beijing by far.

What Is Added By This Report?: In this study, the data of 24,412 participants aged 18-74 years from a large population-based study in Beijing was collected. The overall prevalence of MetS among Beijing residents was 24.5%. The prevalence was 35.2% in males and 15.4% in females.

What Are The Implications For Public Health Practice?: Effective public health strategies should target males, people with older age, lower education, higher body mass index, smokers, those who drink alcohol, those who are unemployed or retired, and those who live in rural areas on MetS prevention and control.
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http://dx.doi.org/10.46234/ccdcw2022.138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339350PMC
July 2022

Nitric oxide electrochemical reduction reaction on transition metal-doped MoSiN monolayers.

Phys Chem Chem Phys 2022 Aug 10;24(31):18943-18951. Epub 2022 Aug 10.

Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710054, P. R. China.

Nitric oxide electrochemical reduction (NOER) reactions are usually catalyzed by noble metals. However, the commercial applications are limited by the low atomic utilization and high price, which prompt researchers to turn their attentions to single-atom catalysts (SACs). Recently, a novel two-dimensional semiconducting material MoSiN (MSN) has been synthesized and is suitable for the substrate of SACs due to its high stability, carrier mobility and mechanical strength. Herein, we employed first principles calculations to investigate the catalytic properties of transition metal doped MoSiN monolayers (labelled as TM-MSN, where TM is a transition metal atom from 3d to 5d except Y, Tc, Cd, La-Lu and Hg) in NO reduction. The calculated results demonstrate that the introduction of Zr, Pd, Pt, Mn, Au, or Mo atoms can greatly improve the catalytic NOER performance of a pristine MSN monolayer. Zr-MSN and Pt-MSN monolayers at low coverage exhibit the most superior catalytic activity and selectivity for NH production with a limiting potential of 0 and -0.10 V. This work may help guide the application of MSN monolayer in the area of energy conversion.
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http://dx.doi.org/10.1039/d2cp01500eDOI Listing
August 2022

Omicron-specific mRNA vaccine elicits potent immune responses in mice, hamsters, and nonhuman primates.

Cell Res 2022 Aug 1. Epub 2022 Aug 1.

Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

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http://dx.doi.org/10.1038/s41422-022-00706-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340695PMC
August 2022

Breast cancer cell-derived extracellular vesicles promote CD8 T cell exhaustion via TGF-β type II receptor signaling.

Nat Commun 2022 Aug 1;13(1):4461. Epub 2022 Aug 1.

Institutes of Biology and Medical Science, Soochow University, Suzhou, China.

Cancer immunotherapies have shown clinical success in various types of tumors but the patient response rate is low, particularly in breast cancer. Here we report that malignant breast cancer cells can transfer active TGF-β type II receptor (TβRII) via tumor-derived extracellular vesicles (TEV) and thereby stimulate TGF-β signaling in recipient cells. Up-take of extracellular vesicle-TβRII (EV-TβRII) in low-grade tumor cells initiates epithelial-to-mesenchymal transition (EMT), thus reinforcing cancer stemness and increasing metastasis in intracardial xenograft and orthotopic transplantation models. EV-TβRII delivered as cargo to CD8 T cells induces the activation of SMAD3 which we demonstrated to associate and cooperate with TCF1 transcription factor to impose CD8 T cell exhaustion, resulting in failure of immunotherapy. The levels of TβRII circulating extracellular vesicles (crEV) appears to correlate with tumor burden, metastasis and patient survival, thereby serve as a non-invasive screening tool to detect malignant breast tumor stages. Thus, our findings not only identify a possible mechanism by which breast cancer cells can promote T cell exhaustion and dampen host anti-tumor immunity, but may also identify a target for immune therapy against the most devastating breast tumors.
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http://dx.doi.org/10.1038/s41467-022-31250-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343611PMC
August 2022

IGF-1 Promotes Epithelial-Mesenchymal Transition of Lens Epithelial Cells That Is Conferred by miR-3666 Loss.

Contrast Media Mol Imaging 2022 14;2022:5383146. Epub 2022 Jul 14.

The First Affiliated Hospital of Harbin Medical University, Ophthalmology Department, Harbin, China.

The abnormal proliferation, migration, and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs) are the main reasons for vision loss caused by posterior capsular opacification (PCO) after cataract surgery. Insulin-like growth factor-1 (IGF-1) was found to be associated with the pathogenesis of cataracts, but its biological role in PCO is poorly understood. In the present study, IGF-1 overexpression facilitated the proliferation, migration, and EMT, whereas knockdown of IGF-1 markedly suppressed the proliferation, migration, and TGF-2-induced EMT of LECs. Additionally, to evaluate valuable microRNAs (miRNAs) which target IGF-1 to modulate LEC-EMT, we predicted miR-3666 might regulate IGF-1 by binding its 3'UTR according to the bioinformatics database. Furthermore, we verified that miR-3666 directly targeted IGF-1 by luciferase reporter assay. By using miR-3666 mimics, cell proliferation, migration, and invasion were suppressed, while being enhanced by the reduction of miR-3666. Knockout of IGF1 reverses the effect of the miR-3666 inhibitor on the malignant behavior of LECs. These results indicate the role of miR-3666/IGF-1 in LEC-EMT that offers new strategies for the therapy and prevention of PCO.
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http://dx.doi.org/10.1155/2022/5383146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303496PMC
August 2022

Cerebral venous sinus thrombosis in a patient with Klebsiella pneumoniae primary liver abscess: a case report.

BMC Neurol 2022 Jul 30;22(1):286. Epub 2022 Jul 30.

Emergency Department and Department of Infectious Diseases, Huashan Hospital of Fudan University, Shanghai, 200040, China.

Background: Liver abscess is a common emergency in the emergency department. However, cerebral venous sinus thrombosis (CVST) is a rare and serious cerebrovascular disease. Cases of CVST in patients with Klebsiella pneumoniae primary liver abscess (KLA) have not been described in the literature. We report a case of CVST in patients with KLA.

Case Presentation: A 54-year-old male patient came to our department with a fever for 2 days and altered mental status for 1 day. Abdominal computed tomography (CT) and liver magnetic resonance imaging (MRI) revealed multiple liver abscesses. The blood culture was identified as Klebsiella pneumoniae sepsis. Head contrast-enhanced MRI and magnetic resonance venography (MRV) imaging showed multiple thrombus formation in the right transverse sinus and sigmoid sinus. The patient's infection and thrombosis were controlled within one week of multidisciplinary comprehensive treatment such as antibiotic and antithrombotic therapy, and a good clinical recovery during the 1-month follow-up.

Conclusion: CVST after liver abscess is rare, clinicians should be aware of this complication and vigilant for the possibility of bacterial meningitis. The underlying mechanisms need to be further studied.
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http://dx.doi.org/10.1186/s12883-022-02806-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338478PMC
July 2022

High-throughput computational screening of hypothetical metal-organic frameworks with open copper sites for CO/H separation.

Phys Chem Chem Phys 2022 Aug 10;24(31):18764-18776. Epub 2022 Aug 10.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, P. R. China.

It is challenging to identify the optimal metal-organic framework (MOF) adsorbents for gas adsorption and membrane-based separation from the large-scale material databases. The high-throughput computational screening (HTCS) method was adopted to discover the optimal materials for CO/H separation from thousands of MOFs. First, a hierarchical strategy was used to select 1092 MOFs from 13 512 MOFs, and their adsorption capacity towards the equimolar CO/H mixture at 298 K and 10 bar was further calculated using the grand canonical Monte Carlo (GCMC) simulations. The results show that those MOFs with  topology and organic linker 1,2,4,5-tetrazine are conducive to exhibiting high performance CO/H adsorption separation among top-100 MOFs with high performance. The MOFs with pore limited diameter (PLD), largest cavity diameter (LCD), gravimetrical surface area (GSA), and void fraction in the range of 4-12 Å, 5-12 Å, 5500-6500 m g and 0.80-0.85, respectively, have high adsorption capacity towards CO. Second, the dynamic adsorption properties of the top-4 MOFs were simulated by the breakthrough curves of the binary (CO/H) and quinary (CO/H/CH/CO/N) mixtures in the fixed adsorption bed. MOF-4641 exhibits a high breakthrough time of 130 for the quinary mixture. Finally, the adsorption mechanism of CO in the top-4 MOFs was investigated by the radial distribution function (RDF), the mass center probability density distribution, The atomic insights from HTCS and breakthrough curve predictions in this work will be helpful in developing novel porous materials and obtaining superior CO separation performance.
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http://dx.doi.org/10.1039/d2cp01139eDOI Listing
August 2022

Multi-Phenotypic Exosome Secretion Profiling Microfluidic Platform for Exploring Single-Cell Heterogeneity.

Small Methods 2022 Jul 28:e2200717. Epub 2022 Jul 28.

Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, 266000, China.

Cellular phenotypic and functional heterogeneities have advanced cancer evolution and treatment resistance. Although exosome-bound proteins reflect cellular functions, single-cell exosomes are rarely profiled owing to the lack of effective platforms. Herein, the authors developed an integrated microfluidic platform consisting of a single-cell trapping chip and a spatially coded antibody barcode chip for the multiplexed outline of exosome secretion by single cells. Using this platform, five phenotypic exosomes of over 1 000 single cells are simultaneously profiled, in addition to inflammatory factor secretion from the same single cell. Also, a robust analysis workflow for single-cell secretion profiling is proposed to explore the intercellular heterogeneity, which integrated unsupervised clustering and linear clustering. When applied to the tumor cell lines of epithelial-origin and normal epithelial cell lines, the strategy identifies functionally heterogeneous subpopulations with unique secretion patterns. Notably, special functional cell subsets for unique phenotypic exosomes (HSP70 , EPCAM ) are found within ovarian tumor cells. The strategy proposed offers a new analysis approach for cellular differential exosome secretion at single-cell resolution using inflammatory factors, ultimately reinforcing the understanding of cell-to-cell heterogeneity and tumor landscape, and providing a valuable universal platform for single-cell biomarker exploration in biological and clinical research.
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http://dx.doi.org/10.1002/smtd.202200717DOI Listing
July 2022

Fe-Catalyzed Amidation of Allylic Alcohols via Borrowing Hydrogen Catalysis.

Chemistry 2022 Jul 28. Epub 2022 Jul 28.

Shaanxi Normal University, School of Chemistry and Chemical Engineering, South Chang'an Road, 710062, Xi'an, CHINA.

Allylic amines are useful building blocks in organic synthesis. The development of green and efficient methods for the preparation of allylic amines are of great importance. An Fe-catalyzed amidation of allylic alcohols with chiral tert -butysulfinamide via a borrowing hydrogen pathway has been developed. With water as the only by-product, a range of synthetically useful chiral sulfinamide olefin derivatives (30 examples) were obtained under mild reaction conditions. The reaction can be performed at gram-scale and the products could serve as chiral ligands for asymmetric catalysis. Mechanistic studies suggest that the reaction proceeds via an Fe-catalyzed borrowing hydrogen process, which is different from most of the reported allylic amination reactions.
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http://dx.doi.org/10.1002/chem.202201829DOI Listing
July 2022

Influence of zeolite confinement effects on cation-π interactions in methanol-to-hydrocarbon conversion.

Chem Commun (Camb) 2022 Jul 28. Epub 2022 Jul 28.

National Center for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.

By using 2D C-C correlation MAS NMR spectroscopy and DFT calculations, the nature of cation-π interactions between cyclopentenyl cations and benzene was clarified over H-ZSM-5, H-β and H-SSZ-13 zeolites. The cation-π interactions are favored over H-β and H-SSZ-13 with large channels or cages. The zeolite structure is identified to affect the arrangements of cyclopentenyl cations and benzene in the confined environment, leading to different extents of overlapping of positive-negative charge centers and cation-π interaction strength. The stronger cation-π interactions facilitate the bimolecular reactions between cyclopentenyl cations and benzene and the formation of coke species.
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http://dx.doi.org/10.1039/d2cc02216hDOI Listing
July 2022

An Underwater Acoustic Network Positioning Method Based on Spatial-Temporal Self-Calibration.

Sensors (Basel) 2022 Jul 26;22(15). Epub 2022 Jul 26.

National Key Laboratory of Science and Technology on Sonar, Hangzhou Applied Acoustics Research Institute, Hangzhou 310000, China.

The emergence of underwater acoustic networks has greatly improved the potential capabilities of marine environment detection. In underwater acoustic network applications, node location is a basic and important task, and node location information is the guarantee for the completion of various underwater tasks. Most of the current underwater positioning models do not consider the influence of the uneven underwater medium or the uncertainty of the position of the network beacon modem, which will reduce the accuracy of the positioning results. This paper proposes an underwater acoustic network positioning method based on spatial-temporal self-calibration. This method can automatically calibrate the space position of the beacon modem using only the GPS position and depth sensor information obtained in real-time. Under the asynchronous system, the influence of the inhomogeneity of the underwater medium is analyzed, and the unscented Kalman algorithm is used to estimate the position of underwater mobile nodes. Finally, the effectiveness of this method is verified by simulation and sea trials.
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http://dx.doi.org/10.3390/s22155571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329755PMC
July 2022

A high mobility air-stable n-type organic small molecule semiconductor with high UV-visible-to-NIR photoresponse.

Light Sci Appl 2022 Jul 27;11(1):236. Epub 2022 Jul 27.

Institute of Advanced Materials and School of Chemistry and Chemical Engineering, Southeast University, 211189, Nanjing, China.

An organic semiconductor with high carrier mobility and efficient light absorption over a wide spectral range is of the most important yet challenging material for constructing a broadband responsive organic photodetector. However, the development of such organic semiconductors, especially for air-stable n-type organic small molecule semiconductors, is still at an early stage. Here we report the fabrication of high-performance n-type semiconducting crystalline nanosheets and the development of air-stable field-effect transistors, phototransistors, with high response over a broad spectrum. The n-type small molecule semiconductor is assembled into a crystalline nanosheet based on the solvent-phase interfacial self-assembly method. N-type field-effect transistors with high electron mobility are fabricated and their electrical performances exhibit excellent air stability. Impressively, the demonstrated phototransistors exhibit an ultrahigh responsivity over a wide spectral range from 365 to 940 nm, with a maximum photoresponsivity of 9.2 × 10 A W and specific detectivity of 5.26 × 10 Jones, which is the best performance among the reported n-type organic small molecule-based phototransistors.
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http://dx.doi.org/10.1038/s41377-022-00936-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329299PMC
July 2022

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS.

Metabolites 2022 Jul 26;12(8). Epub 2022 Jul 26.

College of Tropical Crops, Hainan University, Haikou 570228, China.

Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles and non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS and UHPLC-MS/MS in two coconut varieties (Hainan Tall, HT and Green Dwarf, GD). Four metabolite classes of volatiles were associated with good aroma including hydrocarbons, benzenoids, alcohols and esters, and these volatiles were generally higher in GD, especially at 7 and 9 months of coconut growth. Pathway-based metabolomics revealed that flavonols and their derivatives were significantly enriched in HT, and some of these metabolites were key determinants of HT flesh bitterness, including kaempferol 7--glucoside, a known bitter metabolite. Despite the overall accumulation of amino acids, including L-alanine, L-serine and L-methionine in GD, comparative metabolomics revealed that HT flesh provides a higher content of vitamins than GD. This study sheds light on the metabolic pathways and key metabolites differentiating the flesh flavor quality and nutritional value among coconut varieties, and reveals the possible mechanisms of flavor formation and regulation in coconut fruits.
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http://dx.doi.org/10.3390/metabo12080691DOI Listing
July 2022

Effects of Applying Different Doses of Selenite to Soil and Foliar at Different Growth Stage on Selenium Content and Yield of Different Oat Varieties.

Plants (Basel) 2022 Jul 8;11(14). Epub 2022 Jul 8.

College of Agricultural, Shanxi Agricultural University, No. 1, Mingxian South Road, Taigu District, Jinzhong 030810, China.

(1) Background: With the increase in people's consumption of processed oat products, the production of selenium (Se)-enriched oat has become a possibility to supplement the human body with Se. Therefore, the effects of various factors on the Se-enriched ability and yield of different oat varieties were comprehensively studied. (2) Methods: cv."Pinyan 5" and cv."Bayou 18" were applied at the stem-elongation stage and heading stage in the Jinzhong (JZ), and cv."Bayou 1" and cv."Jinyan 18" were applied at the heading stage and flowering stage in the northwestern Shanxi (JXB) with different doses of NaSeO (0, 5.48, 10.96, 21.92, 43.84, 65.76, 98.64, 0, 5.48, 10.96, 21.92, 43.84, 65.76, 98.64, 147.96 g hm) by soil application and foliar spraying. (3) Results: The grain Se content and yield of oat were affected by the variety, Se application dose, stage and method of Se supplementation. Additionally, the Se content in oat grain was positively correlated with the Se application dose while the yield of oat first increased and then decreased with the Se application dose. (4) Conclusions: In the JZ and JXB, 21.92 g hm and 43.84 g hm Se was sprayed on the leaves of cv."Bayou 18" and cv."Bayou 1" at the heading stage, respectively, was the most effective Se biofortification program.
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http://dx.doi.org/10.3390/plants11141810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324387PMC
July 2022

Noble Metal-Based Catalysts with Core-Shell Structure for Oxygen Reduction Reaction: Progress and Prospective.

Nanomaterials (Basel) 2022 Jul 19;12(14). Epub 2022 Jul 19.

State Key Laboratory of Reliability and Intelligence Electrical Equipment, Hebei University of Technology, Tianjin 300130, China.

With the deterioration of the ecological environment and the depletion of fossil energy, fuel cells, representing a new generation of clean energy, have received widespread attention. This review summarized recent progress in noble metal-based core-shell catalysts for oxygen reduction reactions (ORRs) in proton exchange membrane fuel cells (PEMFCs). The novel testing methods, performance evaluation parameters and research methods of ORR were briefly introduced. The effects of the preparation method, temperature, kinds of doping elements and the number of shell layers on the ORR performances of noble metal-based core-shell catalysts were highlighted. The difficulties of mass production and the high cost of noble metal-based core-shell nanostructured ORR catalysts were also summarized. Thus, in order to promote the commercialization of noble metal-based core-shell catalysts, research directions and prospects on the further development of high performance ORR catalysts with simple synthesis and low cost are presented.
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http://dx.doi.org/10.3390/nano12142480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316484PMC
July 2022

Effect of Annealing Temperature on Electrical Properties of ZTO Thin-Film Transistors.

Nanomaterials (Basel) 2022 Jul 13;12(14). Epub 2022 Jul 13.

Key Laboratory for Comprehensive Energy Saving of Cold Regions Architecture of Ministry of Education, Jilin Jianzhu University, Changchun 130118, China.

A high-performance ZnSnO (ZTO) thin-film transistor (TFT) was fabricated, with ZTO deposited by rf magnetron sputtering. XPS was used to analyze and study the effects of different annealing temperatures on the element composition and valence state of ZTO films. Then, the influence mechanism of annealing treatment on the electrical properties of ZTO thin films was analyzed. The results show that, with an increase in annealing temperature, the amount of metal bonding with oxygen increases first and then decreases, while the oxygen vacancy decreases first and then increases. Further analysis on the ratio of Sn is presented. Electrical results show that the TFT annealed at 600 °C exhibits the best performance. It exhibits high saturation mobilities (μ) up to 12.64 cmVs, a threshold voltage (V) of -6.61 V, a large on/off current ratio (I/I) of 1.87 × 10, and an excellent subthreshold swing (SS) of 0.79 V/Decade.
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http://dx.doi.org/10.3390/nano12142397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325120PMC
July 2022

Growth, Yield and Photosynthetic Performance of Winter Wheat as Affected by Co-Application of Nitrogen Fertilizer and Organic Manures.

Life (Basel) 2022 Jul 6;12(7). Epub 2022 Jul 6.

Agricultural Institute, Centre for Agricultural Research, ELKH, 2462 Martonvásár, Hungary.

The application of organic manures was found to be beneficial, however, the integrated use of organic manures with chemical nitrogen fertilizers has proven more sustainable in increasing the photosynthetic attributes and grain yield of the winter-wheat crop. A multi-factor split-plot design was adopted, nitrogen and manure fertilizer treatments were set in the sub-plots, including nitrogen-gradient treatment of T1:0 kg N ha, T2:100 kg N ha, T3:200 kg N ha, and T4:300 kg N ha (pure nitrogen -fertilizer application) The 25% reduction in nitrogen combined with the manure-fertilizer application includes T5:75 kg N ha nitrogen and 25 kg N ha manure, T6:150 kg N ha nitrogen and 50 kg N ha manure, and T7:225 kg N ha nitrogen and 75 kg N ha manure. The maximum results of the total chlorophyll content and photosynthetic rate were 5.73 mg/g FW and 68.13 m mol m s, observed under T4 in Zhongmai 175, as compared to Jindong 22 at the heading stage. However, the maximum results of intercellular CO concentration were 1998.47 μmol mol, observed under T3 in Jindong 22, as compared to Zhongmai 175 at the tillering stage. The maximum results of LAI were 5.35 (cm), observed under T7 in Jindong 22, as compared to Zhongmai 175 at the booting stage. However, the maximum results of Tr and Gs were 6.31 mmol HO m s and 0.90 HO mol m s, respectively, observed under T7 in Zhongmai 175 as compared to Jindong 22 at the flowering stage. The results revealed that grain yield 8696.93 kg ha, grains spike 51.33 (g), and 1000-grain weight 39.27 (g) were significantly higher, under T3 in Zhongmai 175, as compared to Jindong 22. Moreover, the spike number plot of 656.67 m was significantly higher in Jindong 22, as compared to Zhongmai 175. It was concluded from the study that the combined application of nitrogen and manure fertilizers in winter wheat is significant for enhancing seed at the jointing and flowering stages. For increased grain yield and higher economic return, Zhongmai 175 outperformed the other cultivars examined. This research brings awareness toward the nitrogen-fertilizer-management approach established for farmers' practice, which might be observed as an instruction to increase agricultural management for the winter-wheat-growth season.
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http://dx.doi.org/10.3390/life12071000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319553PMC
July 2022

Privacy-Preserving Design of Scalar LQG Control.

Entropy (Basel) 2022 Jun 22;24(7). Epub 2022 Jun 22.

School of Electronics and Information Engineering, Tongji University, Shanghai 201804, China.

This paper studies the agent identity privacy problem in the scalar linear quadratic Gaussian (LQG) control system. The agent identity is a binary hypothesis: Agent A or Agent B. An eavesdropper is assumed to make a hypothesis testing the agent identity based on the intercepted environment state sequence. The privacy risk is measured by the Kullback-Leibler divergence between the probability distributions of state sequences under two hypotheses. By taking into account both the accumulative control reward and privacy risk, an optimization problem of the policy of Agent B is formulated. This paper shows that the optimal deterministic privacy-preserving LQG policy of Agent B is a linear mapping. A sufficient condition is given to guarantee that the optimal deterministic privacy-preserving policy is time-invariant in the asymptotic regime. It is also shown that adding an independent Gaussian random process noise to the linear mapping of the optimal deterministic privacy-preserving policy cannot improve the performance of Agent B. The numerical experiments justify the theoretic results and illustrate the reward-privacy trade-off.
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http://dx.doi.org/10.3390/e24070856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323139PMC
June 2022
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