Publications by authors named "Chao Shi"

319 Publications

α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback.

Front Pharmacol 2021 7;12:692806. Epub 2021 Jul 7.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, China.

A previously identified anti-rheumatic compound -mangostin (MAN) possesses notable metabolism regulatory properties. In this study, we investigated the immune implication of MAN-altered fat metabolism on adjuvant-induced arthritis (AIA) in rats. Seven days after AIA induction, the rats received oral treatment of MAN at 50 mg/kg/day for 30 days. Metabolic indicators and basic clinical parameters were evaluated using samples collected on day 20 and 38 since immunization. Expression of nicotinamide phosphoribosyltransferase (NAMPT), sirtuin 1 (SIRT1), peroxisome proliferator activated receptor gamma (PPAR-), stearoyl-coa desaturase 1 (SCD-1), toll like receptor 4 (TLR4), prostaglandin-endoperoxide synthase 2 (COX-2), ()-JNK, ()-p65 and IL-1β were investigated by either RT-qPCR or immunobloting methods. In experiments, we treated (pre)-adipocytes with monocytes/macrophages and MAN, and investigated the changes of macrophages brought by pre-adipocytes co-culture. Generally, MAN restored the impaired fat anabolism in AIA rats, indicated by increased fat reservoir, leptin and adiponectin secretion, and PPAR- and SCD-1 expression. Meanwhile, it decreased circulating IL-1β and IL-6 levels, restored serological lipid profile changes, and relieved oxidative stresses, demonstrating potent therapeutic effects on AIA. AIA rats-derived monocytes inhibited mRNA PPAR- and SCD-1 expression in pre-adipocytes. Contrarily, MAN facilitated adipocyte differentiation , and increased free fatty acids production. It also significantly increased PPAR- and SCD-1 expression, which can be abrogated by PPAR- inhibitor T0070907. Similarly, lipopolysaccharide-primed macrophages inhibited PPAR- expression in the co-cultured pre-adipocytes, which was reversed by MAN. In the same co-culture system, lipopolysaccharide-induced inflammation was amplified by the co-existence of pre-adipocytes. More secretion of IL-1β and IL-6 and higher levels expression of COX-2, p-JNK, p-p65 and TLR4 were observed in lipopolysaccharide-treated macrophages when co-cultured by pre-adipocytes. The intensified inflammatory situation was eased by MAN. The treatment with pre-adipocytes culture medium achieved similar effects. Medium from lipopolysaccharide-treated adipocytes promoted IL-1β, IL-6 and MCP-1 production in separately cultured macrophages, and COX-2, p-JNK, p-p65 and TLR4 expression were increased at the meantime. MAN treatment on pre-adipocytes impaired these changes. It suggests that fat anabolism in AIA rats was deficient due to increased energy expenditure caused by inflammatory conditions. MAN restored fat metabolism homeostasis by up-regulating PPAR-, and reshaped secretion profile of adipocytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.692806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293671PMC
July 2021

Characterization of auxin transporter , and gene families in pineapple and evaluation of expression profiles during reproductive development and under abiotic stresses.

PeerJ 2021 22;9:e11410. Epub 2021 Jun 22.

Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.

Polar auxin transport in plant is mediated by influx and efflux transporters, which are encoded by , and genes, respectively. The auxin transporter gene families have been characterized in several species from monocots and eudicots. However, a genome-wide overview of auxin transporter gene families in pineapple is not yet available. In this study, we identified a total of three genes, 12 genes, and seven genes in the pineapple genome, which were variably located on 15 chromosomes. The exon-intron structure of these genes and properties of deduced proteins were relatively conserved within the same family. Most protein motifs were widespread in the AUX, PIN or PILS proteins, whereas a few motifs were absent in only one or two proteins. Analysis of the expression profiles of these genes elucidated that several genes exhibited either preferential or tissue-specific expression patterns in vegetative and/or reproductive tissues. was specifically expressed in the early developmental ovules, while and were strongly expressed in stamens and ovules. , , , and were abundantly expressed in stamens. Furthermore, qRT-PCR results showed that several genes in these families were responsive to various abiotic stresses. Comparative analysis indicated that the genes with close evolutionary relationships among pineapple, rice and exhibited similar expression patterns. Overexpression of the in rescued the phenotype in , and resulted in increased lateral roots in WT. These results will provide new insights into auxin transporter genes of pineapple and facilitate our understanding of their roles in pineapple growth and development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.11410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231336PMC
June 2021

Dense GAN and multi-layer attention based lesion segmentation method for COVID-19 CT images.

Biomed Signal Process Control 2021 Aug 23;69:102901. Epub 2021 Jun 23.

Department of Medical Imaging, Zhejiang Hospital, Hangzhou 310013, China.

As the COVID-19 virus spreads around the world, testing and screening of patients have become a headache for governments. With the accumulation of clinical diagnostic data, the imaging big data features of COVID-19 are gradually clear, and CT imaging diagnosis results become more important. To obtain clear lesion information from the CT images of patients' lungs is helpful for doctors to adopt effective medical methods, and at the same time, is helpful to screen the patients with real infection. Deep learning image segmentation is widely used in the field of medical image segmentation. However, there are some challenges in using deep learning to segment the lung lesions of COVID-19 patients. Since image segmentation requires the labeling of lesion information on a pixel by pixel basis, most professional radiologists need to screen and diagnose patients on the front line, and they do not have enough energy to label a large amount of image data. In this paper, an improved Dense GAN to expand data set is developed, and a multi-layer attention mechanism method, combined with U-Net's COVID-19 pulmonary CT image segmentation, is proposed. The experimental results showed that the segmentation method proposed in this paper improved the segmentation accuracy of COVID-19 pulmonary medical CT image by comparing with other image segmentation methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bspc.2021.102901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220920PMC
August 2021

Coronary Artery-Left Ventricular Fistula with Giant Right Coronary Aneurysm: A Case Report and Literature Review.

Heart Surg Forum 2021 May 11;24(3):E433-E436. Epub 2021 May 11.

Department of Cardiac Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, P.R. China.

Right coronary artery-left ventricular (RCA-LV) fistula with associated giant right coronary artery aneurysm (CAA) is an extremely rare cardiac condition. This case study presents a patient with a large left ventricle (LV) and a giant right CAA with a maximal inner diameter of approximately 56.6 mm and an inner diameter of approximately 22 mm at its communication with the left ventricle. The patient underwent surgical management, involving suturing of the proximal end of the CAA and coronary artery bypass grafting (CABG). RCA-LV fistula with a giant right CAA may involve serious complications, such as thrombosis, rupture, and heart failure. Therefore, it is necessary to establish effective management strategies for this condition. Although this case is not unique, it serves as an illustrative example of the implementation of a classic surgical treatment method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1532/hsf.3777DOI Listing
May 2021

The complete chloroplast genome sequence of (Benth.) S. Moore. (Asteraceae).

Mitochondrial DNA B Resour 2021 Jun 3;6(7):1834-1836. Epub 2021 Jun 3.

Kunming Medical University Haiyuan College, Kunming, China.

The complete chloroplast (cp) genome of was sequenced and assembled for the first time. In this study, the total genome size is 150,596 bp in length and demonstrates a typical quadripartite structure containing a large single copy (LSC, 82,575 bp) and a small single copy (SSC, 18,293 bp), separated by a pair of inverted repeats (IRa, IRb) of 24,864 bp. The G + C content of this cp genome was 37.21%. Gene annotation analysis identified 130 genes including 85 protein-coding genes, 37 transfer RNA, and 8 ribosomal RNA genes. The maximum-likelihood phylogenetic analysis result showed that was closely related to in the phylogenetic relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1934165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183548PMC
June 2021

The complete chloroplast genome sequence of (Verbenaceae).

Mitochondrial DNA B Resour 2021 Jun 3;6(7):1832-1833. Epub 2021 Jun 3.

College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, China.

In this study, the complete chloroplast (cp) genome of was assembled using Illumina sequencing data. The complete cp genome is 149,869 bp in length, including a pair of invert repeats (IRA and IRB) regions of 22,839 bp, large single-copy (LSC) region of 86,201 bp, and small single-copy (SSC) region of 17,990 bp. The G + C content of this cp genome was 38.26%. A total of 128 genes were predicted in the genome, including 83 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis confirmed the phylogenetic relationship between and other representative species of Verbenaceae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1934164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183526PMC
June 2021

The complete chloroplast genome sequence of Lam. (Poaceae).

Mitochondrial DNA B Resour 2021 May 24;6(6):1747-1748. Epub 2021 May 24.

College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, China.

The complete chloroplast (cp) genome of was sequenced and assembled for the first time. In this study, The total genome size is 134,511 bp in length and demonstrates a typical quadripartite structure containing a large single copy (LSC, 95,227 bp) and a small single copy (SSC, 12,306 bp), separated by a pair of inverted repeats (IRa, IRb) of 13,489 bp. The G + C content of this chloroplast genome was 38.76%. Gene annotation analysis identified 130 genes including 84 protein-coding genes, 38 transfer RNA, and 8 ribosomal RNA genes. The maximum-likelihood phylogenetic analysis result showed that was closely related to in the phylogenetic relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1931503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158213PMC
May 2021

Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q against Typhimurium.

Foods 2021 May 27;10(6). Epub 2021 May 27.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.

Coenzyme Q (CoQ) has anti-inflammatory and anti-tumor effects; however, the antimicrobial and antibiofilm activities of CoQ against serovar Typhimurium are unknown. Thus, we investigated the bacteriostatic and antibiofilm activities, along with the underlying mechanism, of CoQ against . Typhimurium. The minimum inhibitory concentration (MIC) of CoQ against serovars Typhimurium was 0.1-0.2 mg/mL (549-1098 µM), and CoQ at MIC and 2MIC decreased viable . Typhimurium counts below detectable limits within 6 and 4 h, respectively. CoQ at 20MIC (4 mg/mL) reduced . Typhimurium on raw chicken by 1.5 log CFU/cm within 6 h. CoQ effectively disrupted cell membrane integrity and induced morphological changes in the cell, resulting in hyperpolarization, decreased intracellular ATP concentrations, and cellular constituents leakage. Biofilm-associated . Typhimurium cells were killed by CoQ treatment. These findings suggest that CoQ could be applied as a natural antibacterial substance for use against . Typhimurium by the food industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods10061211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230291PMC
May 2021

Fermented Deer Blood Ameliorates Intense Exercise-Induced Fatigue via Modulating Small Intestine Microbiota and Metabolites in Mice.

Nutrients 2021 May 3;13(5). Epub 2021 May 3.

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.

Intense and excessive exercise-induced fatigue has become an important health issue and can damage intestinal health. Deer blood, as a food byproduct with nutritional value, has been found to restore physical strength. However, little is known about the antifatigue effect of fermented deer blood (FDB) on intense exercise mice. The purpose of the present study is to investigate the antifatigue effect of FDB, and whether this effect is correlated with the altered small intestinal microbiota and metabolites in exercise mice. In this study, 5-week-old male C57BL/6J mice are given treadmill exercise with or without FDB supplementation (30 and 150 mg/kg/d) for 3 weeks. FDB significantly reduces metabolic byproduct accumulation, liver and intestinal damage, and enhances glycogen storage and antioxidant capacity in intense exercise mice. Moreover, FDB restructures the small intestinal microbiota by increasing the abundance of probiotics and butyric acid producing bacteria and decreasing the abundance of pathogenic bacteria. FDB also regulates the levels of metabolites involved in TCA cycle and amino acid metabolism in urine and small intestine content. Correlation analysis shows that FDB-modulated microbiota is highly associated with its antifatigue effect. FDB may ameliorate fatigue and intestinal injury through targeting small intestinal microbiota.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu13051543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147844PMC
May 2021

Effect of 405-nm light-emitting diode on environmental tolerance of Cronobacter sakazakii in powdered infant formula.

Food Res Int 2021 06 24;144:110343. Epub 2021 Mar 24.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Cronobacter sakazakii is an opportunistic pathogen that can survive extreme desiccation, heat, acid, and osmotic stress. This can increase the risk of infection, resulting in severe diseases, mainly in neonates. The inactivation effect of 405 ± 5-nm light-emitting diode (LED) illumination on C. sakazakii with different initial concentrations and C. sakazakii strains isolated from powdered infant formula (PIF) and baby rice cereal (BRC) were firstly evaluated. Then, the effect of 405 ± 5-nm LED on the tolerance of diverse environmental conditions of C. sakazakii in PIF was investigated. Conditions involving desiccation [PIF, Water activity (a): 0.2-0.5], heat (45, 50, and 55 °C), acid (simulated gastric fluid: SGF, pH 4.75 ± 0.25), and bile salt (0.2%, bile salt solution) were used to study the effects of 405-nm LED on C. sakazakii resistance. The transcription levels of ten tolerance-associated genes and changes in bacterial cell membrane were examined to understand the response of C. sakazakii to LED illumination. The results showed that 405-nm LED effectively inactivated C. sakazakii ATCC 29544 with initial concentration from 8 to 1 log CFU/g in PIF and strains isolated from PIF and BRC. Moreover, 405-nm LED could decrease the tolerance of C. sakazakii in PIF to desiccation, heat treatment at 50 and 55 °C, SGF, and bile salt to different degrees, but the resistance to the heat treatment at 45 °C was not influenced by LED illumination. In addition, the transcription levels of the ten tolerance-associated genes measured in the LED-illuminated C. sakazakii cells were significantly downregulated compared with those in unilluminated controls. The damage on cell membrane was confirmed for LED-treated cells by LIVE/DEAD® assay. These results indicate that 405-nm LED illumination may be effective at reducing the environmental resistance of C. sakazakii in PIF. Furthermore, this study suggests the potential for applying 405-nm LED technology in the prevention and control of pathogens in food processing, production, and storage environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2021.110343DOI Listing
June 2021

NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1.

Am J Transl Res 2021 15;13(4):2111-2126. Epub 2021 Apr 15.

Department of Cardiovascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei Province, China.

Purpose: To clarify the regulatory effect of Nuclear-enriched abundant transcript 1 (NEAT1) on abdominal aortic aneurysm (AAA) model rats and isolated endothelial progenitor cells (EPCs).

Methods: The AAA rat model was established by CaCl stimulation, and overexpressed NEAT1 was injected into rats through tail vein. Abdominal aorta lesions and numbers of EPCs in tissues and peripheral blood were examined by hematoxylin-eosin, immunofluorescence and flow cytometry. The extracted EPCs were identified by microscopy, DiI-ac-LDL staining and flow cytometry. Effect of overexpressed/silencing NEAT1 on the viability, migration, tube formation and VEGF content of EPCs was investigated by MTT-, wound-healing, tube formation assays and ELISA, respectively. The expressions of NEAT1, miR-204-5p, Angiopoietin-1 (Ang-1)/ERK pathway were determined by qRT-PCR and Western blot as needed. The targeting relationships between NEAT1 and miR-204-5p, and miR-204-5p and Ang-1 were predicted on starBase, TargetScan and confirmed by dual-luciferase experiments. The mutual regulation effect was studied through rescue experiments.

Results: Overexpressed NEAT1 not only reduced inflammatory infiltration and increased the number of EPCs in abdominal aorta and peripheral blood, but also promoted the viability, migration, tube formation of EPCs, increased VEGF content and upregulated the expression of the Ang-1/ERK pathway in EPCs. However, silencing NEAT1 produced opposite results. NEAT1 targeting miR-204-5p inhibited the functional effects of miR-204-5p on of EPCs. Overexpressed/silencing Ang-1 partially reversed the effects of NEAT1 or miR-204-5p on the characteristics of EPCs.

Conclusion: NEAT1 competitively binds with miR-204-5p and up-regulates Ang-1 expression in EPCs to effectively improve the proliferation, migration and angiogenesis of EPCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129351PMC
April 2021

Detection of Epstein-Barr virus by a rapid and simple accelerated denaturation bubble-mediated strand exchange amplification method.

Anal Methods 2021 06 19;13(22):2519-2526. Epub 2021 May 19.

Department of Pathogenic Biology, School of Basic Medicine, College of Life Sciences, Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, the Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, P. R. China.

Epstein-Barr Virus (EBV) is a tumor-associated virus naturally transmitted through saliva. This virus is the pathogen of infectious mononucleosis, which is closely related to the occurrence of nasopharyngeal carcinoma (NPC) and childhood lymphoma. Although a majority of EBV infected individuals exhibited good tolerance after primary infection, those who carry a viral load greater than the clinical cutoff value (COV), the upper level in healthy carriers, still suffer a high risk of cancer. Herein, a simple, rapid, and effective method, accelerated strand exchange amplification (ASEA), was developed for EBV detection, which could offer a strategy for non-invasive testing of EBV in saliva samples instead of blood samples as in traditional serology based methods and avoid bleeding during diagnosis. This approach could distinguish the genomic DNA of EBV and other species in saliva, and its limit of detection was as low as 1000 copies per mL, which was lower than the COV of EBV. Moreover, DNA extracted from saliva samples (n = 50) was employed as a template for EBV detection via qPCR and ASEA, the result of which showed that ASEA exhibited comparable sensitivity and specificity for actual sample diagnosis. Additionally, similar to conventional PCR, this method requires only one pair of primers and could be performed using a conventional fluorescence instrument, which makes this method easy to accomplish. Therefore, this rapid and effective method has the potential to provide rapid screening platforms for individuals with a high EBV load.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ay00347jDOI Listing
June 2021

Persistence of Antibody and Cellular Immune Responses in COVID-19 patients over Nine Months after Infection.

J Infect Dis 2021 May 12. Epub 2021 May 12.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

Background: The duration of humoral and T and cell response after the infection of SARS-CoV-2 remains unclear.

Methods: We performed a cross-sectional study to assess the virus-specific antibody and memory T and B cell responses in COVID-19 patients up to 343 days after infection. Neutralizing antibodies and antibodies against the receptor-binding domain, spike, and nucleoprotein of SARS-CoV-2 were measured. Virus-specific memory T and B cell responses were analyzed.

Results: We enrolled 59 COVID-19 patients, including 38 moderate, 16 mild, and five asymptomatic patients; 31 (52.5%) were men, and 28 (47.5%) were women. The median age was 41 (interquartile range [IQR]: 30-55). The median day from symptom onset to enrollment was 317 days (range 257 to 343 days). We found that approximately 90% of patients still have detectable IgG antibodies against spike and nucleocapsid proteins and neutralizing antibodies against pseudovirus, whereas ~60% of patients had detectable IgG antibodies against receptor binding domain and surrogate virus-neutralizing antibodies. SARS-CoV-2-specific IgG + memory B cell and IFN-γ secreting T cell responses were detectable in over 70% of patients.

Conclusions: SARS-CoV-2-specific immune memory response persists in most patients nearly one year after infection, which provides a promising sign for prevention from reinfection and vaccination strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/infdis/jiab255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243600PMC
May 2021

Internal Migration and Leprosy in Shanghai from 2000 to 2019: an Epidemiological Study of New Cases.

Acta Derm Venereol 2021 May 19;101(5):adv00459. Epub 2021 May 19.

Department of infectious Diseases, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China.

Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Massive internal migration from rural to urban areas poses new challenges for leprosy control in Shanghai, China. This retrospective epidemiological study examined new cases of leprosy diagnosed in Shanghai from 2000 to 2019, with emphasis on internal migration cases. There were 145 cases of leprosy in the study period; the majority of cases (89.0%) were internal migrants. Migrant cases had a mean of 25.4 months lag time from onset of symptoms to diagnosis, which was significantly longer than that of resident cases (mean 10.8 months, p < 0.001). Greater lag time from the first visit to diagnosis was observed in migrant cases (mean 23.2 months) compared with resident cases (mean 9.4 months, p < 0.001). A large majority of cases (91.0%) had been misdiagnosed. Internal migrant cases were responsible for most incidences of leprosy in Shanghai. They often did not receive timely diagnosis and treatment, which may have an adverse impact on the prevention of epidemic leprosy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2340/00015555-3821DOI Listing
May 2021

Development of Boron-Doped Mesoporous Carbon Materials for Use in CO Capture and Electrochemical Generation of HO.

ACS Omega 2021 Mar 22;6(12):8438-8446. Epub 2021 Mar 22.

Inner Mongolia Key Laboratory of Green Catalysis, College of Chemistry and Environmental Science, Inner Mongolia Normal University, Hohhot 010022, China.

Mesoporous carbon materials have been increasingly studied due to their large specific surface area and good chemical stability. Optimizing their functionality through a doping modification can broaden their application in many fields. Herein, a series of B-doped mesoporous carbon materials are prepared by a convenient hydrothermal synthesis using F127 as the template and boric acid as the boron source. The whole material preparation process meets the requirements of green chemistry. Notably, the prepared carbon materials not only exhibit good electrocatalytic oxygen reduction to hydrogen peroxide in alkaline media but also have an excellent CO adsorption capacity (up to 121.34 mg/g) at 303 K and atmospheric pressure. These results show that the prepared samples can be utilized as multifunctional materials for handling a variety of environmental issues.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c00197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015076PMC
March 2021

The complete chloroplast genome sequence of .

Mitochondrial DNA B Resour 2021 Mar 16;6(3):927-928. Epub 2021 Mar 16.

College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, China.

In this study, the complete chloroplast genome of was assembled using Illumina sequencing data. The complete chloroplast (cp) genome is 134,556 bp in length, including a pair of invert repeats (IRA and IRB) regions of 20,797 bp, large single-copy (LSC) region of 80,615 bp, and small single-copy (SSC) region of 12,347 bp. A total of 129 genes were predicted in the genome, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis confirmed the phylogenetic relationship between and other representative species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1888331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971287PMC
March 2021

Assembly and comparative analysis of the complete mitochondrial genome of Suaeda glauca.

BMC Genomics 2021 Mar 9;22(1):167. Epub 2021 Mar 9.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: Suaeda glauca (S. glauca) is a halophyte widely distributed in saline and sandy beaches, with strong saline-alkali tolerance. It is also admired as a landscape plant with high development prospects and scientific research value. The S. glauca chloroplast (cp) genome has recently been reported; however, the mitochondria (mt) genome is still unexplored.

Results: The mt genome of S. glauca were assembled based on the reads from Pacbio and Illumina sequencing platforms. The circular mt genome of S. glauca has a length of 474,330 bp. The base composition of the S. glauca mt genome showed A (28.00%), T (27.93%), C (21.62%), and G (22.45%). S. glauca mt genome contains 61 genes, including 27 protein-coding genes, 29 tRNA genes, and 5 rRNA genes. The sequence repeats, RNA editing, and gene migration from cp to mt were observed in S. glauca mt genome. Phylogenetic analysis based on the mt genomes of S. glauca and other 28 taxa reflects an exact evolutionary and taxonomic status of S. glauca. Furthermore, the investigation on mt genome characteristics, including genome size, GC contents, genome organization, and gene repeats of S. gulaca genome, was investigated compared to other land plants, indicating the variation of the mt genome in plants. However, the subsequently Ka/Ks analysis revealed that most of the protein-coding genes in mt genome had undergone negative selections, reflecting the importance of those genes in the mt genomes.

Conclusions: In this study, we reported the mt genome assembly and annotation of a halophytic model plant S. glauca. The subsequent analysis provided us a comprehensive understanding of the S. glauca mt genome, which might facilitate the research on the salt-tolerant plant species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-021-07490-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941912PMC
March 2021

Antibacterial Activity and Mechanism of Coenzyme Q Against .

Foodborne Pathog Dis 2021 Jun 11;18(6):398-404. Epub 2021 Mar 11.

College of Food Science and Engineering, Northwest A&F University, Yangling, China.

Coenzyme Q (CoQ) is a natural compound found in , which has a variety of biological activities. Here, the antibacterial activity and possible antibacterial mechanism of CoQ against were investigated. The antibacterial effect was evaluated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, and by assessing bacterial survival and the effect on the growth of after CoQ treatment in Luria-Bertani (LB) broth. To reveal the antibacterial mechanism of CoQ, changes in intracellular adenosine triphosphate (ATP) concentration, membrane potential, and bacterial protein content, as well as effects on cell morphology and membrane integrity, were investigated. Both the MICs and MBCs of CoQ against were 0.1 mg/mL. After treatment of (6.5 log colony-forming units/mL) with 0.1 mg/mL of CoQ in LB broth for 3 h, the number of viable cells dropped below the detection limit. In addition, CoQ treatment resulted in the reduction in intracellular ATP concentration, cell membrane hyperpolarization, decreased bacterial protein concentrations, and damage to cell membrane integrity and cellular morphology. These results indicated that CoQ has effective antibacterial activity against , suggesting potential applications in food industry safety.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/fpd.2020.2884DOI Listing
June 2021

Effect of slightly acidic electrolyzed water on natural Enterobacteriaceae reduction and seed germination in the production of alfalfa sprouts.

Food Microbiol 2021 Aug 9;97:103414. Epub 2020 Jan 9.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China; School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, 1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, China. Electronic address:

Microbial contamination of sprouts commonly occurs because of the pathogens present on and in the seeds and the optimal conditions for bacteria growth provided during the germination and sprouting processes. This study examined the decontamination effect of slightly acidic electrolyzed water (SAEW), a 'generally recognized as safe' (GRAS) disinfectant, in the production process of alfalfa sprouts. SAEW with various available chlorine concentrations (ACC, 25, 35, 45 mg/L) and different pH levels (5.0, 5.7 and 6.4) was used to soak seeds for different length of time (0.5 and 6 h), after which the variations in natural Enterobacteriaceae, water absorption and seed germination (germination rate, weight and length of sprouts) were determined. The results showed that when the seeds were soaked with SAEW, albeit with different ACC (25, 35 and 45 mg/L) and pH levels (5.0, 5.7 and 6.4), a significant reduction of Enterobacteriaceae and no negative effect on sprout quality was observed. The water absorption and germination rates were also not significantly adversely affected by SAEW soaking. These findings suggest that SAEW could be used to decontaminate natural Enterobacteriaceae in the production of alfalfa sprouts, with no negative side effects on the alfalfa seeds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fm.2020.103414DOI Listing
August 2021

Positive feedback of SuFu negating protein 1 on Hedgehog signaling promotes colorectal tumor growth.

Cell Death Dis 2021 02 19;12(2):199. Epub 2021 Feb 19.

Center for Experimental Medicine, The First Affiliated Hospital of Nanchang University, 330006, Nanchang, Jiangxi, China.

Hedgehog (Hh) signaling plays a critical role in embryogenesis and tissue homeostasis, and its deregulation has been associated with tumor growth. The tumor suppressor SuFu inhibits Hh signaling by preventing the nuclear translocation of Gli and suppressing cell proliferation. Regulation of SuFu activity and stability is key to controlling Hh signaling. Here, we unveil SuFu Negating Protein 1 (SNEP1) as a novel Hh target, that enhances the ubiquitination and proteasomal degradation of SuFu and thus promotes Hh signaling. We further show that the E3 ubiquitin ligase LNX1 plays a critical role in the SNEP1-mediated degradation of SuFu. Accordingly, SNEP1 promotes colorectal cancer (CRC) cell proliferation and tumor growth. High levels of SNEP1 are detected in CRC tissues and are well correlated with poor prognosis in CRC patients. Moreover, SNEP1 overexpression reduces sensitivity to anti-Hh inhibitor in CRC cells. Altogether, our findings demonstrate that SNEP1 acts as a novel feedback regulator of Hh signaling by destabilizing SuFu and promoting tumor growth and anti-Hh resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03487-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896051PMC
February 2021

Resonant-type piezoelectric inertial drive mechanism with asymmetric inertial masses.

Rev Sci Instrum 2021 Jan;92(1):015003

School of Instrument Science and Opto-Electronics Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.

Resonant-type piezoelectric impact motors can improve the output capability effectively. A new construction of asymmetric inertial masses is proposed to synthesize approximate saw-tooth wave resonant vibration with matched anti-phase and in-phase modes. A prototype is designed, manufactured, and tested. With 40 V, 400 Hz exciting voltage for the anti-phase mode and 16 V, 800 Hz exciting voltage for the in-phase mode, the prototype provides a maximum no-load velocity of 17.2 mm/s and a maximum output power of 0.72 mW with 100 mN load. The velocity and output power of the prototype in the proposed resonant state are near three orders of magnitude higher than those in the traditional quasi-static state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1063/5.0030775DOI Listing
January 2021

Reusable Hyperbranched Polyethylenimine-Functionalized Ethyl Cellulose Film for the Removal of Phosphate with Easy Separation.

ACS Omega 2021 Jan 24;6(1):505-515. Epub 2020 Dec 24.

School of Engineering, Zhejiang A & F University, 666 Wusu Street, Hangzhou 311300, PR China.

The design of a reusable film adsorbent with easy solid-liquid separation for the removal of phosphate is necessary and significant but remains hugely challenging. Herein, the hyperbranched polyethylenimine-functionalized ethyl cellulose (HPEI-EC) film was successfully synthesized by a one-step solution-casting method. The structure and elemental composition of the HPEI-EC film were characterized by Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. The phosphate adsorption capacity of the HPEI-EC film was 15.53 mg g, which is 12 times higher than that of EC. Significantly, the elongation at break of the HPEI-EC film was 13.43%, which is higher than that of the EC film (8.9%), and the HPEI-EC film had a considerable tensile strength of 13.21 MPa. Such good mechanical properties of the HPEI-EC film bring about the advantage of the saturated HPEI-EC film, allowing it to be easily taken out using a pair of tweezers, which significantly reduces the operation time and saves the cost in the application process. Furthermore, the HPEI-EC film possessed good reusability, and 71.6% of the original adsorption capacity of phosphate was retained even after five cycles. Moreover, the electrostatic interaction between protonated the amine group (-NH ) and the phosphate ion (PO ) is mainly responsible for the adsorption process. This study presents a low-cost and reusable film adsorbent for the effective removal of phosphate from water and provides an easy solid-liquid separation method for use in the adsorption field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c04955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807744PMC
January 2021

Identification of key pathways and biomarkers in anaplastic thyroid cancer using an integrated analysis.

J BUON 2020 Nov-Dec;25(6):2690-2699

Department of Clinical Medicine, The Second Clinical Medical College, Nanchang University, Nanchang, Jiangxi, China.

Purpose: Thyroid carcinoma (THCA) is one of the most common endocrine tumours with high morbidity worldwide. Anaplastic thyroid cancer (ATC) is the most fatal and has the poorest prognosis of the four THCA types, as it lacks effective treatments. Early screening of ATC is problematic and so identifying ATC biomarkers is increasingly crucial.

Methods: We performed a systematic search of the thyroid transcriptome in the Gene Expression Omnibus (GEO) database and an integrative analysis of gene expression profiles. Moreover, we conducted a pathway enrichment analysis in ATC using the WEB-based GEne SeT AnaLysis Toolkit. We identified the intersections of all the differentially expressed genes (DEGs) between ATC and normal samples and DEGs between ATC and non-ATC samples in the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). Finally, we used Cytoscape software to visualize the protein-protein interaction (PPI) network.

Results: Six gene expression datasets containing 131 thyroid cancer samples and 98 normal control samples were collected to identify the significant DEGs. A total of 1489 DEGs were identified between ATC and normal samples, and 522 DEGs between ATC and non-ATC samples. ATC showed a greater association with the cell cycle. The Principal component analysis (PCA) results revealed 222 genes with substantial contributions to the identification of ATC.

Conclusion: Cell cycle plays a decisive role in the high mortality rate of ATC. TOP2A, NUSAP1, PBK, KIF15, CENPF, CEP55, CDK1, CCNB2, CDCA8 and CDC20 were identified as hub genes.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2021

B- and N-Embedded π-Conjugation Units Tuning Intermolecular Interactions and Optical Properties of Platinum(II) Complexes.

Inorg Chem 2021 Jan 30;60(2):525-534. Epub 2020 Dec 30.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, People's Republic of China.

A new series of neutral and cationic platinum(II) complexes containing a B- or N-embedded π-conjugation unit has been prepared. Notably, significantly different intermolecular interactions (Pt-Pt, π-π, head to tail, and head to head) and interesting optical properties exist in these complexes, which can be attributed to the difference in spatial structures and π-electron properties between B- and N-embedded π-conjugation units. Unexpectedly, under a hypoxic atmosphere, N-embedded neutral complex PtNacac can display a distinct dual-emission with both fluorescence and phosphorescence, whereas only a single fluorescence emission was observed in the air, which is different from the B-embedded neutral complex PtBacac with only a single phosphorescence emission at any atmosphere, as well confirmed by lifetime measurement and oxygen sensing experiments. DFT calculations reveal that unusual ligand-to-metal charge transfer (LMCT) excited state character and low spin orbit coupling (SOC) elements can be found in N-embedded complexes due to the strong electron-donating ability of the N-embedded unit. Based on this, as a novel ratiometric oxygen probe with a simple structure, PtNacac can be successfully used to examine intracellular oxygen levels by monitoring both fluorescence and phosphorescence signals via ratiometric photoluminescence imaging and time-resolved luminescence imaging (TRLI) technology. This work provides a completely new idea for designing fluorescence/phosphorescence dual-emissive complexes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.0c03078DOI Listing
January 2021

Inactivation Efficacy of 405 nm LED Against Biofilm.

Front Microbiol 2020 27;11:610077. Epub 2020 Nov 27.

College of Food Science and Engineering, Northwest A&F University, Yangling, China.

The objectives of this study were to evaluate the inactivation efficacy of a 405-nm light-emitting diode (LED) against biofilm formed on stainless steel and to determine the sensitivity change of illuminated biofilm to food industrial disinfectants. The results showed that LED illumination significantly reduced the population of viable biofilm cells, showing reduction of 2.0 log (25°C), 2.5 log (10°C), and 2.0 log (4°C) between the non-illuminated and LED-illuminated groups at 4 h. Images of confocal laser scanning microscopy and scanning electron microscopy revealed the architectural damage to the biofilm caused by LED illumination, which involved destruction of the stereoscopic conformation of the biofilm. Moreover, the loss of biofilm components (mainly polysaccharide and protein) was revealed by attenuated total reflection Fourier-transformed infrared spectroscopy, and the downregulation of genes involved in biofilm formation was confirmed by real time quantitative PCR analysis, with greatest difference observed in . In addition, the sensitivity of illuminated-biofilm cells to disinfectant treatment was found to significantly increased, showing the greatest sensitivity change with 1.5 log reduction between non-LED and LED treatment biofilms in the CHX-treated group. These results indicated that 405 nm LED illumination was effective at inactivating biofilm adhering to stainless steel. Therefore, the present study suggests the potential of 405 nm LED technology in controlling biofilms in food processing and storage, minimizing the risk of contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.610077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728857PMC
November 2020

Development and Validation of a Novel Nomogram for Predicting Tumor-Distant-Metastasis in Patients with Early T1-2 Stage Lung Adenocarcinoma.

Ther Clin Risk Manag 2020 10;16:1213-1225. Epub 2020 Dec 10.

Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China.

Background: Distant metastasis in early T1-2 (diameter≤5 cm) stage lung adenocarcinoma (ET-LUAD) patients largely affect treatment strategies in clinical practice. However, the associated mechanism remains unclear and related studies is less. This study aimed to establish and validate a novel nomogram to predict the risk of distant metastasis in ET-LUAD.

Methods: A total of 258 patients diagnosed with ET-LUAD and not receiving any treatment were recruited into this study. The patients were randomly divided into a training cohort and validation cohort in a ratio of 1:2. Univariate and multivariate logistic regression analysis was used to select the most significant predictive risk factors associated with distant metastasis in the training cohort. The established nomogram was validated by the consistency index (C-index), calibration curve, and decision curve analysis (DCA).

Results: There were 124 patients with confirmed distant metastasis and 134 patients with non-distant metastases ET-LUAD were enrolled in the study. Multivariate logistic hazards regression analysis identified independent risk factors associated with distant metastasis to include platelet-to-lymphocyte ratios (PLR), lactate dehydrogenase (LDH), neural-specific enolase (NSE), carcinoembryonic antigen (CEA) and cytokeratin 19 fragments (Cyfra211), which were included in the establishment of the nomogram. The nomogram achieved a high consistency (C-index=0.792), good calibration, and high clinical application value in the validation cohort.

Conclusion: The established nomogram can be used to predict distant metastasis in high-risk ET-LUAD nonmetastasis patients and can also be used by doctors to guide preventive and individualized treatment for ET-LUAD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/TCRM.S272748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735943PMC
December 2020

The Effect of Ambient Temperature on Infectious Diarrhea and Diarrhea-like Illness in Wuxi, China.

Disaster Med Public Health Prep 2020 Nov 20:1-7. Epub 2020 Nov 20.

Department of Disease Control, Wuxi Center for Disease Control and Prevention, Wuxi, Jiangsu Province, China.

Background: The disease burden of infectious diarrhea cannot be underestimated. Its seasonal patterns indicate that weather patterns may play an important role and have an important effect on it. The objective of this study was to clarify the relationship between temperature and infectious diarrhea, and diarrhea-like illness.

Methods: Distributed lag non-linear model, which was based on the definition of a cross-basis, was used to examine the effect.

Results: Viral diarrhea usually had high incidence in autumn-winter and spring with a peak at -6°C; Norovirus circulated throughout the year with an insignificant peak at 8°C, while related bacteria usually tested positive in summer and peaked at 22°C. The lag-response curve of the proportion of diarrhea-like cases in outpatient and emergency cases revealed that at -6°C, with the lag days increasing, the proportion increased. Similar phenomena were observed at the beginning of the curves of virus and bacterial positive rate, showing that the risk increased as the lag days increased, peaking on days 16 and 9, respectively. The shape of lag-response curve of norovirus positive rate was different from others, presenting m-type, with 2 peaks on day 3 and day 18.

Conclusion: Weather patterns should be taken into account when developing surveillance programs and formulating relevant public health intervention strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/dmp.2020.340DOI Listing
November 2020

Above room-temperature dielectric switching and semiconducting properties of a layered organic-inorganic hybrid compound: (CHN)Pb(NO).

Dalton Trans 2020 Dec;49(46):16860-16865

Chaotic Matter Science Research Center, Jiangxi University of Science and Technology, Ganzhou 330000, Jiangxi, China.

The well-studied star compound, CH3NH3PbI3, has attracted plenty of attention because of its remarkable optical and electrical properties. Consequently, new switching multifunctional hybrid compounds can be widely used in many fields such as solar cells, light-emitting diodes, optical data storage and so on. Therefore, switching multifunctional hybrid compounds with dielectric and semiconducting properties simultaneously will also find roles in the next generation of optoelectronic coupling materials. In fact, discovering an effective method to synthesize (multi)functional hybrid materials remains a pressing challenge. Thanks to the "quasi-spherical theory" proposed by Xiong et al., we used 7-azabicyclo[2.2.1]heptane as the quasi-spherical cation to construct molecule-based crystalline materials that exhibit responsive properties. Then, we tried to exploit the knowledge of crystal engineering and coordination chemistry to explain (multi)functional molecular materials. A layered organic-inorganic hybrid compound, (C6H12N)2Pb(NO3)4 (1), was grown and its dielectric switching property and semiconducting behaviour were investigated. Insights from differential scanning calorimetry measurements, variable-temperature X-ray structural studies, and dielectric spectroscopy revealed the origin of the phase transition, which is related to the motion of the organic ammonium and inorganic framework in solid-state crystals. Furthermore, 1 is also a wide bandgap semiconductor with an optical bandgap of 3.53 eV. The realization of switching and semiconducting properties simultaneously in layered Pb-based perovskites has a great significance toward research into hybrid compounds and the development of dielectric-optoelectronic integrated materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0dt03206aDOI Listing
December 2020
-->