Publications by authors named "Chao Li"

3,471 Publications

  • Page 1 of 1

Global malaria infection risk from climate change.

Environ Res 2022 Aug 5:114028. Epub 2022 Aug 5.

Urban Institute, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan. Electronic address:

As a long-standing public health issue, malaria still severely affects many parts of the world, especially Africa. With greenhouse gas emissions, temperatures continue to rise. Based on diverse shared socioeconomic pathways (SSPs), future temperatures can be estimated. However, the impacts of climate change on malaria infection rates in all epidemic regions are unknown. Here, we estimate the differences in global malaria infection rates predicted under different SSPs during several periods as well as malaria infection case changes (MICCs) resulting from those differences. Our results indicate that the global MICCs resulting from the conversion from SSP1-2.6 to SSP2-4.5, to SSP3-7.0, and to SSP5-8.5 are 6.506 (with a 95% uncertainty interval [UI] of 6.150-6.861) million, 3.655 (3.416-3.894) million, and 2.823 (2.635-3.012) million, respectively, from 2021 to 2040; these values represent increases of 2.699%, 1.517%, and 1.171%, respectively, compared to the 241 million infection cases reported in 2020. Temperatures increases will adversely affect malaria the most in Africa during the 2021-2040 period. From 2081 to 2100, the MICCs obtained for the three scenario shifts listed above are -79.109 (-83.626 to -74.591) million, -238.337 (-251.920 to -0.141) million, and -162.692 (-174.628 to -150.757) million, corresponding to increases of -32.825%, -98.895%, and -67.507%, respectively. Climate change will increase the danger and risks associated with malaria in the most vulnerable regions in the near term, thus aggravating the difficulty of eliminating malaria. Reducing GHG emissions is a potential pathway to protecting people from malaria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2022.114028DOI Listing
August 2022

Site-selective sulfation of N-glycans by human GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis of sulfated antibody glycoforms.

Bioorg Chem 2022 Aug 1;128:106070. Epub 2022 Aug 1.

Department of Chemistry and Biochemistry, University of Maryland, 8051 Regents Drive, College Park, MD 20742, United States. Electronic address:

Sulfation is a common modification of glycans and glycoproteins. Sulfated N-glycans have been identified in various glycoproteins and implicated for biological functions, but in vitro synthesis of structurally well-defined full length sulfated N-glycans remains to be described. We report here the first in vitro enzymatic sulfation of biantennary complex type N-glycans by recombinant human CHST2 (GlcNAc-6-O-sulfotransferase 1, GlcNAc6ST-1). We found that the sulfotransferase showed high antennary preference and could selectively sulfate the GlcNAc moiety located on the Manα1,3Man arm of the biantennary N-glycan. The glycan chain was further elongated by bacterial β1,4 galactosyltransferase from Neiserria meningitidis and human β1,4 galactosyltransferase IV(B4GALT4), which led to the formation of different sulfated N-glycans. Using rituximab as a model IgG antibody, we further demonstrated that the sulfated N-glycans could be efficiently transferred to an intact antibody by using a chemoenzymatic Fc glycan remodeling method, providing homogeneous sulfated glycoforms of antibodies. Preliminary binding analysis indicated that sulfation did not affect the apparent affinity of the antibody for FcγIIIa receptor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2022.106070DOI Listing
August 2022

Focus on the tumor microenvironment: A seedbed for neuroendocrine prostate .

Front Cell Dev Biol 2022 22;10:955669. Epub 2022 Jul 22.

Department of Urology, Xiangya Hospital, Central South University, Changsha, China.

The tumor microenvironment (TME) is a microecology consisting of tumor and mesenchymal cells and extracellular matrices. The TME plays important regulatory roles in tumor proliferation, invasion, metastasis, and differentiation. Neuroendocrine differentiation (NED) is a mechanism by which castration resistance develops in advanced prostate cancer (PCa). NED is induced after androgen deprivation therapy and neuroendocrine prostate cancer (NEPC) is established finally. NEPC has poor prognosis and short overall survival and is a major cause of death in patients with PCa. Both the cellular and non-cellular components of the TME regulate and induce NEPC formation through various pathways. Insights into the roles of the TME in NEPC evolution, growth, and progression have increased over the past few years. These novel insights will help refine the NEPC formation model and lay the foundation for the discovery of new NEPC therapies targeting the TME.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2022.955669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355504PMC
July 2022

A preliminary study on preparation, characterization, and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava.

J Food Biochem 2022 Aug 7:e14371. Epub 2022 Aug 7.

Department of Biological and Food Engineering, School of Chemical Engineering, Xiangtan University, Xiangtan, China.

Polysaccharides derived from edible mushrooms were sources of new prebiotic compounds. Limited studies of their prebiotic effects, as well as the presence of residual dark colors, impede their use as prebiotics in the food industry. To boost the prebiotic value of polysaccharides from the edible mushroom Ramaria flava, a decolorization method, and the physicochemical characterization and prebiotic potential of the decolorized polysaccharide (DRFP) were investigated in this study. The reversed micelle system consisting of n-hexanol/isooctane (3:7, v/v) and 200 mM surfactant (CTAB) was an appropriate decolorized method for R. flava crude polysaccharide. That decolorized polysaccharide was 101.68 kDa and contained glucose, galactose, mannose, fucose, xylose, rhamnose, arabinose, and glucuronic acid in a ratio of 40.61:26.97:17.72:7.78:6.31:0.11:0.06:0.44. Furthermore, DRFP exhibited typical shear-thinning behavior and possessed good thermal stability. And 98.76% of DRFP was allowed to transit through the oral cavity and gastrointestinal tract nearly indigestibly in vitro fermentation. It also revealed a prebiotic availability through stimulating Lactobacillus rhamnosus proliferation and inducing enterocoel acidification. When utilized as a carbon source, DRFP significantly improves the production of short-chain fatty acids by L. rhamnosus, particularly acetic, propionic, isobutyric, and hexanoic acids. Therefore, this work suggests the further application of R. flava polysaccharides as emerging prebiotics, which may be used as an ingredient in functional food and nutraceutical products. PRACTICAL APPLICATIONS: Prebiotics could regulate gut microbial community and are closely associated with host health. This work reported that a decolorized polysaccharide (DRFP) prepared from the edible mushroom Ramaria flava was indigestible and could improve Lactobacillus rhamnosus proliferation and short-chain fatty acid production, which could provide useful information for the application of DRFP as a prebiotic additive in the food industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.14371DOI Listing
August 2022

Two-dimensional defective black phosphorus/BiVO nanoheterojunctions for molecular nitrogen activation.

J Colloid Interface Sci 2022 Jul 20;628(Pt A):378-388. Epub 2022 Jul 20.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China. Electronic address:

Solar-driven transfer of nitrogen into ammonia is still challenging as a result of the strong dipole moment of the NN triple bond. Molecular nitrogen activation to construct the reactive active nitrogen species (RNS) is the key aspect for the highly efficient solar N conversion. Herein, we selected black phosphorus-BiVO (BP-BiVO) as a model system to clarify the significance of the inherent defective state to the molecular nitrogen activation over the formed heterojunctions. Oxygen vacancies (OVs) induced BP-BiVO heterojunctions (OV-BP-BiVO) were controllably fabricated through quasi-reverse-emulsion approach. OVs in the lattice of the heterojunctions were introduced by the pulsed laser irradiation, which to a great extent determined the kinetics, energetics, and mechanism of the photocatalytic N reduction. Systematic experiments revealed that the optimized heterojunction interfaces and rich OVs states led to superior ability in the activation of active molecular nitrogen (N → RNS → NH). This work brightens defective and interfacial perspectives for the construction of efficient heterojunctions to produce reactive nitrogen species with high photocatalytic activity, and extend their potentials to a wide variety of other inorganic/organic transformations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2022.07.092DOI Listing
July 2022

Preparation, characterization and bioavailability studies of Tegillarca granosa hemoglobin and its glycosylated products.

Int J Biol Macromol 2022 Aug 2;219:11-20. Epub 2022 Aug 2.

College of Food and Pharmaceutical Sciences, Ningbo University, 315211, China; Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China. Electronic address:

Iron deficiency anemia (IDA) is a common micronutrient deficiency. Tegillarca granosa (T. granosa) is a good source of iron due to its high content of hemoglobin. The present study aimed to determine the effects of glycosylation on structure, physicochemical characteristics and iron bioavailability of hemoglobin. Using Box-Behnken design and response surface methodology, the optimal conditions for hemoglobin-chitosan glycosylation were obtained: 61.8 °C, pH 6.3, hemoglobin/chitosan mass ratio of 4.3 and reaction time of 15 min. The formation of hemoglobin-chitosan conjugates was verified by SDS-PAGE and fluorescence spectroscopy. The surface hydrophobicity of hemoglobin was reduced by 20.90-65.05 % after glycosylation, along with the observations of elevated water-holding capacity, likely owing to the introduction of hydrophilic groups. Antioxidant capacity of glycosylated products (0.41-0.66 μM Trolox/mg protein) was markedly greater than that of original protein (0.06 μM Trolox/mg protein) due to the formation of brown polymers with antioxidant activity. In addition, glycosylation improved in vitro digestibility of hemoglobin by 41.15-69.09 %, which could be attributed to less β-sheet in secondary structures. Moreover, hemoglobin (324.38 ng/mg) exhibited better iron absorption than FeSO (121.63 ng/mg), with the value being further enhanced by glycosylation (442.73 ng/mg), which may be due to the improved protein digestibility and iron-chelating capacity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2022.07.234DOI Listing
August 2022

Direct Observation of Li-Ion Transport Heterogeneity Induced by Nanoscale Phase Separation in Li-rich Cathodes of Solid-State Batteries.

Angew Chem Int Ed Engl 2022 Aug 5. Epub 2022 Aug 5.

Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences, Department of Energy Science and Energy Technology, Songling Road, 189, 266101, Qingdao City, CHINA.

Li-rich layered oxide (LLO) cathode materials with high specific capacities could significantly enhance the energy density of all-solid-state lithium batteries (ASSLBs). However, the specific practical capacities of LLO materials in ASSLBs are extremely low due to poor initial activation. Here, scanning transmission electron microscopy with in-situ differential phase contrast imaging was first used to study the initial activation mechanism of Li1.2Ni0.13Co0.13Mn0.54O2. Li-ion transport heterogeneity was observed in LLO grains and across the LLO/Li6PS5Cl interface, due to the coexistence of the nanoscale Li2MnO3 and LiNi1/3Co1/3Mn1/3O2 phases. Consequently, the severely constrained activation of Li2MnO3 during the first charging could be attributed to a nanoscale phase separation in LLO, hindering Li-ion transport through its particles, and causing high impedance in the Li2MnO3 domain/Li6PS5Cl interface. This study could facilitate interface design of high-performance LLO-based ASSLBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202209626DOI Listing
August 2022

A Nomogram Based on Hematological Parameters and Clinicopathological Characteristics for Predicting Local-Regional Recurrence After Breast-Conserving Therapy.

Front Oncol 2022 19;12:861210. Epub 2022 Jul 19.

Breast Cancer Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Objectives: The aim of this study was to identify the factors for local-regional recurrence (LRR) after breast-conserving therapy (BCT). We established a practical nomogram to predict the likelihood of LRR after BCT based on hematological parameters and clinicopathological features.

Methods: A retrospective analysis was performed on 2,085 consecutive breast cancer patients who received BCT in Shandong Cancer Hospital from 2006 to 2016, including 1,460 patients in the training cohort and 625 patients in the validation cohort. Univariate and multivariate analyses were performed based on hematological parameters (fibrinogen, platelets, mean platelet volume, neutrophils, monocytes, and lymphocytes) and clinicopathological characteristics to identify the independent factors for LRR. Subsequently, a nomogram for predicting LRR was established by logistic regression analysis. The nomogram was validated in 625 patients in the validation cohort.

Results: During the median follow-up period of 66 months, 44 (3.01%) patients in the training cohort and 19 (3.04%) patients in the validation cohort suffered from LRR. Multivariate analysis showed six independent factors related to LRR, including molecular subtype, pathological N stage, re-resection, radiotherapy or not, platelet count*MPV*fibrinogen (PMF), and neutrophil count/lymphocyte count ratio (NLR). Six variables were entered into logistic regression to establish the nomogram for predicting LRR. The nomogram of LRR showed excellent discrimination and prediction accuracy. The area under the receiver operating characteristic curve (AUC) was 0.89 ( < 0.001, 95% CI = 0.83, 0.95) in the training cohort and 0.88 ( < 0.001, 95% CI = 0.8, 0.96) in the validation cohort. Calibration curves for the prediction model in the training and validation cohorts both demonstrated satisfactory consistency between the nomogram-predicted and actual LRR.

Conclusion: The combination of hematological parameters and clinicopathological characteristics can predict LRR after BCT. The predictive nomogram based on preoperative and postoperative indicators of BCT might serve as a practical tool for individualized prognostication. More prospective studies should be performed to verify the model.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.861210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344968PMC
July 2022

Fumigant toxicity and behavioral inhibition of garlic against red imported fire ants (Solenopsis invicta).

Environ Sci Pollut Res Int 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou, 510642, China.

In order to control the Solenopsis invicta (S. invicta) in a safe and ecofriendly manner, this study evaluated the fumigating activity of garlic and its effect on the behavior of S. invicta. The volatile compounds and chemical constituents from garlic were collected by solid phase micro-extraction and identified by gas chromatography-mass spectrometry (GC-MS). The results showed that garlic contains 21 compounds, and that diallyl disulfide (46.51%) and (E)-1-Allyl-2-(prop-1-en-1-yl) disulfane (34.68%) were the main constituents in garlic. In addition, garlic showed strong fumigant activity and behavioral inhibition against workers. The LC values of diallyl disulfide and methyl allyl disulfide against S. invicta at 12 h were 0.05 and 0.07 μg/L, respectively. The grasping and walking abilities of ants were significantly suppressed. Moreover, minor workers were more susceptible than major workers. Thus, S. invicta can be controlled with garlic and some of its compounds as an eco-friendly approach. Results from the study could provide valuable advances for future fumigant development and possible utility in fumigant use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-022-22091-zDOI Listing
August 2022

Phytochemical profile, bioactivity and prebiotic potential of bound polyphenols released from fruit pomace dietary fiber during digestion and fermentation.

Food Funct 2022 Aug 4. Epub 2022 Aug 4.

School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.

The aim of this study was to elucidate the liberation and phytochemical profile of bound polyphenols present in dietary fiber (RPDF) isolated from fruit pomace during -simulated digestion and colonic fermentation. The variation in the biological activity and prebiotic potential of the released polyphenols from RPDF was investigated. The results showed that the bound polyphenols were released from RPDF at a higher ratio during colonic fermentation than gastrointestinal digestion. The released polyphenols showed antioxidant activity and α-glucosidase inhibitory activity. The colonic fermentation assay showed that compared with dephenolized RPDF (DP-RPDF), RPDF exhibited a significantly stronger prebiotic effect by lowering the to (/) ratio at the phylum level, increasing the relative abundances of beneficial bacteria at the genus level, and improving the production of short chain fatty acids (SCFAs). These findings indicate that the bound polyphenols may act as important functional food ingredients that can contribute to the biological properties of RPDF. In addition, this study provides new insights into the high-value utilization of fruit pomace, which will reduce the wastage of resources and greatly contribute to environmental protection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2fo00823hDOI Listing
August 2022

Combination effect of intraoperative and postoperative intravenous tranexamic acid in hip hemiarthroplasty. A propensity score matched analysis.

Injury 2022 Jul 20. Epub 2022 Jul 20.

Department of Orthopaedic Surgery, Zhongda Hospital, Southeast University, Nanjing, PR China. Electronic address:

Background: Tranexamic acid (TXA) has been proven to reduce perioperative blood loss and the incidence of allogeneic blood transfusion in total joint arthroplasty (TJA). However, there is limited literature on the use of TXA in patients undergoing hip hemiarthroplasty. Furthermore, combination effect of intraoperative and postoperative intravenous TXA has not been investigated extensively. The purpose of this study was to evaluate this combination effect on hip hemiarthroplasty by a propensity score matched analysis (PSMA).

Methods: This is a retrospective cohort study involving hip hemiarthroplasty for femoral neck fracture. All patients received 1g intraoperative intravenous TXA. One group also received 1g intravenous TXA 6h after surgery, while the other group did not. Perioperative blood loss, the incidence of allogeneic blood transfusion and venous thromboembolism (VTE) were collected and compared.

Results: 209 patients who underwent unilateral hip hemiarthroplasty were included. PSMA resulted in 66 matched pairs in each group. There were no significant differences in perioperative blood loss on postoperative day (POD) # 1, 3, 5 (637.2 ± 285.9 versus 653.6 ± 305.5, 726.6 ± 274.2 versus 676.3 ± 313.2, 795.5 ± 291.2 versus 759.9 ± 328.4), the incidence of allogeneic blood transfusion (9.1% versus 12.1%) and VTE (1.5% versus 1.5%) between the two groups (P value> 05) for up to one year.

Conclusion: Intraoperative and postoperative intravenous TXA have no combination effect on reducing perioperative blood loss, the incidence of allogeneic blood transfusion and VTE in patients undergoing hip hemiarthroplasty.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.injury.2022.07.033DOI Listing
July 2022

Human-specific gene CT47 blocks PRMT5 degradation to lead to meiosis arrest.

Cell Death Discov 2022 Aug 2;8(1):345. Epub 2022 Aug 2.

Cambridge-Su Genomic Resource Center, Jiangsu Key Laboratory of Neuropsychiatric Diseases, Medical School of Soochow University, Suzhou, Jiangsu, 215123, China.

Exploring the functions of human-specific genes (HSGs) is challenging due to the lack of a tractable genetic model system. Testosterone is essential for maintaining human spermatogenesis and fertility, but the underlying mechanism is unclear. Here, we identified Cancer/Testis Antigen gene family 47 (CT47) as an essential regulator of human-specific spermatogenesis by stabilizing arginine methyltransferase 5 (PRMT5). A humanized mouse model revealed that CT47 functions to arrest spermatogenesis by interacting with and regulating CT47/PRMT5 accumulation in the nucleus during the leptotene/zygotene-to-pachytene transition of meiosis. We demonstrate that testosterone induces nuclear depletion of CT47/PRMT5 and rescues leptotene-arrested spermatocyte progression in humanized testes. Loss of CT47 in human embryonic stem cells (hESCs) by CRISPR/Cas9 led to an increase in haploid cells but blocked the testosterone-induced increase in haploid cells when hESCs were differentiated into haploid spermatogenic cells. Moreover, CT47 levels were decreased in nonobstructive azoospermia. Together, these results established CT47 as a crucial regulator of human spermatogenesis by preventing meiosis initiation before the testosterone surge.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41420-022-01139-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345867PMC
August 2022

Gemcitabine Plus Anlotinib Is Effective and Safe Compared to Gemcitabine Plus Docetaxel in Advanced Soft Tissue Sarcoma.

Front Oncol 2022 13;12:922127. Epub 2022 Jul 13.

Department of Orthopedics, The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, China.

Objective: The aim of this study is to compare gemcitabine (G) plus docetaxel (D) versus G plus anlotinib (A) for advanced soft tissue sarcoma (STS).

Methods: We retrospectively investigated 122 patients with locally advanced or metastatic STS who were treated with either G+D or G+A between July 2016 and October 2021 and compared the efficacy and toxicity of G+D and G+A. The primary endpoints were median progression-free survival (PFS) and the proportion of patients with grade ≥3 adverse events. We also analyzed differences in the clinical efficacy of G+D and G+A in leiomyosarcoma, and the differences in the clinical efficacy of G+D and G+A as first-line therapy.

Results: Overall, 122 patients were included (81 patients receiving G+D and 41 patients receiving G+A) with a median age of 55 years. The main histological types are leiomyosarcoma, undifferentiated pleomorphic sarcoma, and liposarcoma. After a median follow-up of 25 months, PFS did not differ between patients treated with G+D and those treated with G+A (median PFS: 5.8 months and 6.8 months, = 0.39), and overall survival (OS) was similar (median OS: 14.7 vs. 13.3 months, = 0.75) with a similar objective response rate (18.5% vs. 14.6%, = 0.17), whereas the proportion of patients with grade ≥3 adverse events treated with G+D was significantly higher than those treated with G+A (68% vs. 44%, < 0.05). Subgroup analysis of leiomyosarcoma patients (47.5% of the patients) and first-line treatment patients (46.7% of the patients) shows that PFS was not significantly different between the two groups (LMS: median PFS: 6.5 months vs. 7.5 months, = 0.08; first-line treatment: median PFS: 6.2 months vs. 7.1 months, = 0.51).

Conclusion: Compared with gemcitabine plus docetaxel for advanced STS, gemcitabine plus anlotinib achieved a similar response rate on median PFS and OS, but lower toxicity. These results suggest that gemcitabine plus anlotinib may be an effective and safe strategy for advanced STS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.922127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326030PMC
July 2022

Cerebral endothelial cell derived small extracellular vesicles improve cognitive function in aged diabetic rats.

Front Aging Neurosci 2022 14;14:926485. Epub 2022 Jul 14.

Department of Neurology, Henry Ford Hospital, Detroit, MI, United States.

Small extracellular vesicles (sEVs) mediate cell-cell communication by transferring their cargo biological materials into recipient cells. Diabetes mellitus (DM) induces cerebral vascular dysfunction and neurogenesis impairment, which are associated with cognitive decline and an increased risk of developing dementia. Whether the sEVs are involved in DM-induced cerebral vascular disease, is unknown. Therefore, we studied sEVs derived from cerebral endothelial cells (CEC-sEVs) of aged DM rats (DM-CEC-sEVs) and found that DM-CEC-sEVs robustly inhibited neural stem cell (NSC) generation of new neuroblasts and damaged cerebral endothelial function. Treatment of aged DM-rats with CEC-sEVs derived from adult healthy normal rats (N-CEC-sEVs) ameliorated cognitive deficits and improved cerebral vascular function and enhanced neurogenesis. Intravenously administered N-CEC-sEVs crossed the blood brain barrier and were internalized by neural stem cells in the neurogenic region, which were associated with augmentation of miR-1 and -146a and reduction of myeloid differentiation primary response gene 88 and thrombospondin 1 proteins. In addition, uptake of N-CEC-sEVs by the recipient cells was mediated by clathrin and caveolin dependent endocytosis signaling pathways. The present study provides and evidence that DM-CEC-sEVs induce cerebral vascular dysfunction and neurogenesis impairment and that N-CEC-sEVs have a therapeutic effect on improvement of cognitive function by ameliorating dysfunction of cerebral vessels and increasing neurogenesis in aged DM rats, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2022.926485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330338PMC
July 2022

MLP-Based Regression Prediction Model For Compound Bioactivity.

Front Bioeng Biotechnol 2022 13;10:946329. Epub 2022 Jul 13.

School of Statistics and Mathematics, Zhejiang Gongshang University, Hangzhou, China.

The development of breast cancer is closely linked to the estrogen receptor ERα, which is also considered to be an important target for the treatment of breast cancer. Therefore, compounds that can antagonize ERα activity may be drug candidates for the treatment of breast cancer. In drug development, to save manpower and resources, potential active compounds are often screened by establishing compound activity prediction model. For the 1974 compounds collected, the top 20 molecular descriptors that significantly affected the biological activity were screened using LASSO regression models combined with 10-fold cross-validation method. Further, a regression prediction model based on the MLP fully connected neural network was constructed to predict the bioactivity values of 50 new compounds. To measure the validity of the model, the model loss term was specified as the mean squared error (MSE). The results showed that the MLP-based regression prediction model had a loss value of 0.0146 on the validation set. This model is therefore well trained and the prediction strategy used is valid. The methods developed by this paper may provide a reference for the development of anti-breast cancer drugs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2022.946329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326362PMC
July 2022

Assessment of the Potential of Nakai. in Treating Ethanol-Induced Gastric Ulcer in Rats Based on Metabolomics and Network Analysis.

Front Pharmacol 2022 12;13:810344. Epub 2022 Jul 12.

School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, China.

Gastric ulcer (GU) is one of the most commonly diagnosed diseases worldwide, threatening human health and seriously affecting quality of life. Reports have shown that the Chinese herbal medicine (Thunb.) Nakai (SGN) can treat GU. However, its pharmacological effects deserve further validation; in addition, its mechanism of action is unclear. An acute gastric ulcer (AGU) rat model induced by alcohol was used to evaluate the gastroprotective effect of SGN by analysis of the histopathological changes in stomach tissue and related cytokine levels; the potential mechanisms of action of SGN were investigated via serum metabolomics and network pharmacology. Differential metabolites of rat serum were identified by metabolomics and the metabolic pathways of the identified metabolites were enriched via MetaboAnalyst. Furthermore, the critical ingredients and candidate targets of SGN anti-AGU were elucidated. A compound-reaction-enzyme-gene network was established using Cytoscape version 3.8.2 based on integrated analysis of metabolomics and network pharmacology. Finally, molecular docking was applied to verify the acquired key targets. The results showed that SGN exerted a certain gastroprotective effect via multiple pathways and targets. The effects of SGN were mainly caused by the key active ingredients isofraxidin, rosmarinic, and caffeic acid, which regulate hub targets, such as PTGS2, MAPK1, and KDR, which maintain the homeostasis of related metabolites. Signal pathways involved energy metabolism as well as immune and amino acid metabolism. Overall, the multi-omics techniques were proven to be promising tools in illuminating the mechanism of action of SGN in protecting against diseases. This integrated strategy provides a basis for further research and clinical application of SGN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.810344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315220PMC
July 2022

Two Microsatellite Types Within NAD6 Gene Help to Distinguish Populations and Infer the Migratory Route of the Invasive Fall Armyworm, Spodoptera frugiperda, (Lepidoptera, Noctuidae) in China.

J Econ Entomol 2022 Jul 28. Epub 2022 Jul 28.

Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Shandong, Jinan, China.

Spodoptera frugiperda is a major agricultural pest that has invaded China since January 2019. Given that most of the individuals present in China carried the diagnostic rice-strain mtDNA (COI-RS), there was no efficient method to distinguish populations of S. frugiperda. In this study, we identified and characterized two variant microsatellite alleles in the mitochondrial NAD6 gene of S. frugiperda retrieved from the National Center for Biotechnology Center GenBank. We then sequenced partial NAD6 genes containing the microsatellite region and the diagnostic COI barcoding gene (used to distinguish the corn-strain and the rice-strain) of 429 invasive S. frugiperda individuals that were collected from the main infested regions in China during 2019-2020. Our data indicates that two kinds of interrupted repeat sequences, (ATA)4T(ATA)3 and (ATA)5T(ATA)3, exist in the microsatellite region which we defined as the deletion type (NAD6-D), and the insertion type (NAD6-I) based on the repeat units' differentiation, respectively. The presence of these two microsatellite types in the mtDNA genome of S. frugiperda was further confirmed with the sequencing results in 429 samples. Moreover, NAD6-I and NAD6-D types were both present in individuals with COI-RS, while only NAD6-D type was detected in the COI-CS individuals. Interestingly, the two microsatellite types suggested a possible geographic distribution: the western migratory route (Yunan and Chongqing) was comprised exclusively of NAD6-I type, while both NAD6-I and NAD6-D types were identified in the predicted eastern migration trajectories (Hainan, Guangxi, Shandong, etc.). These results suggested that NAD6-D and NAD6-I types may be useful in distinguishing between populations, analyzing the evolutionary mechanism of mtDNA microsatellite polymorphism, inferring the migratory route of S. frugiperda in China, and developing precise and integrated control strategies for S. frugiperda.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jee/toac114DOI Listing
July 2022

The Impact of Catalpol on Proliferation, Apoptosis, Migration, and Oxidative Stress of Lung Cancer Cells Based on Nrf2/ARE Signaling.

Biomed Res Int 2022 18;2022:5621341. Epub 2022 Jul 18.

Division of Thoracic and Endocrine Surgery, unige.it, 1211 Geneva 4, Switzerland.

The effects of catalpol on lung cancer cell proliferation, apoptosis, migration, and oxidative stress via the Nrf2/ARE signaling pathway are investigated in this work. Catalpol-12 g/mL group, catalpol-24 g/mL group, catalpol-48 g/mL group, catalpol - 48 g/mL + vector group, catalpol - 48 g/mL + Nrf2 group, si-NC group, and si-Nrf2 group were used to split lung cancer cells A549 into control groups. Proliferation was detected using the CCK-8 assay; apoptosis was detected using flow cytometry; migration was detected using the transwell chamber; ROS was distinguished using the DCFHDA method; MDA, SOD, and GSH were detected using the microvolume method; and Cleaved Caspase-3, Cleaved Caspase-9, Nrf2, HO-1, MMP-9, and MMP-2 were detected using the Western blot method. Catalpol 12 g/mL and 24 g/mL-48 g/mL treatment decreased the proliferation activity, migration number, and Nrf2, HO-1, MMP-9, and MMP-2 protein levels of lung cancer cells when compared to the control group. SOD and GSH levels of lung cancer cells were decreased, and MDA and ROS levels were increased. Cleaved caspase-3, cleaved caspase-9 protein expression levels, and apoptosis were boosted ( < 0.05). The proliferation activity, migration number, and protein levels of Nrf2, HO-1, MMP-9, and MMP-2 in the catalpol - 48 g/mL + Nrf2 group were raised compared to the catalpol - 48 g/mL + vector group, whereas there was an apparent drop in the Cleaved Caspase-3, Cleaved Caspase-9, and apoptosis rate. Similarly, SOD and GSH contents increased, whereas MDA and ROS decreased ( < 0.05). The proliferation activity, migration number, and Nrf2, HO-1, MMP-9, and MMP-2 protein levels of lung cancer cells in the si-Nrf2 group were all decreased when compared to the si-NC and control groups. Cleaved Caspase-3 and Cleaved Caspase-9 protein expression, on the other hand, increased as MDA and ROS levels were raised while SOD and GSH levels dropped ( < 0.05). It reveals that catalpol inhibits the Nrf2/ARE signaling pathway, which causes antiproliferation, migration, apoptosis, and oxidative stress in cancer cells of lungs. The rate of apoptosis was also lowered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5621341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313965PMC
July 2022

An Attention-Based CoT-ResNet With Channel Shuffle Mechanism for Classification of Alzheimer's Disease Levels.

Front Aging Neurosci 2022 11;14:930584. Epub 2022 Jul 11.

Key Laboratory of Spectral Imaging Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.

Detection of early morphological changes in the brain and early diagnosis are important for Alzheimer's disease (AD), and high-resolution magnetic resonance imaging (MRI) can be used to help diagnose and predict the disease. In this paper, we proposed two improved ResNet algorithms that introduced the Contextual Transformer (CoT) module, group convolution, and Channel Shuffle mechanism into the traditional ResNet residual blocks. The CoT module is used to replace the 3 × 3 convolution in the residual block to enhance the feature extraction capability of the residual block, while the Channel Shuffle mechanism is used to reorganize the feature maps of different groups in the input layer to improve the communication between the feature maps from different groups. Images of 503 subjects, including 116 healthy controls (HC), 187 subjects with mild cognitive impairment (MCI), and 200 subjects with AD, were selected and collated from the ADNI database, and then, the data were pre-processed and sliced. After that, 10,060 slices were obtained and the three groups of AD, MCI and HC were classified using the improved algorithms. The experiments showed that the refined ResNet-18-based algorithm improved the top-1 accuracy by 2.06%, 0.33%, 1.82%, and 1.52% over the traditional ResNet-18 algorithm for four medical image classification tasks, namely AD: MCI, AD: HC, MCI: HC, and AD: MCI: HC, respectively. The enhanced ResNet-50-based algorithm improved the top-1 accuracy by 1.02%, 2.92%, 3.30%, and 1.31%, respectively, over the traditional ResNet-50 algorithm in four medical image classification tasks, demonstrating the effectiveness of the CoT module replacement and the inclusion of the channel shuffling mechanism, as well as the competitiveness of the improved algorithms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2022.930584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309569PMC
July 2022

Methylation of a MITE insertion in the MdRFNR1-1 promoter is positively associated with its allelic expression in apple in response to drought stress.

Plant Cell 2022 Jul 28. Epub 2022 Jul 28.

State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, China.

Miniature inverted-repeat transposable elements (MITEs) are widely distributed in the plant genome and can be methylated. However, whether DNA methylation of MITEs is associated with induced allelic expression and drought tolerance is unclear. Here, we identified the drought-inducible MdRFNR1 (root-type ferredoxin-NADP+ oxidoreductase) gene in apple (Malus domestica). MdRFNR1 plays a positive role in drought tolerance by regulating the redox system, including increasing NADP+ accumulation and catalase and peroxidase activities and decreasing NADPH levels. Sequence analysis identified a MITE insertion (MITE-MdRF1) in the promoter of MdRFNR1-1 but not the MdRFNR1-2 allele. MdRFNR1-1 but not MdRFNR1-2 expression was significantly induced by drought stress, which was positively associated with the MITE-MdRF1 insertion and its DNA methylation. The methylated MITE-MdRF1 is recognized by the transcriptional anti-silencing factors MdSUVH1 and MdSUVH3, which recruit the DNAJ domain-containing proteins MdDNAJ1, MdDNAJ2, and MdDNAJ5, thereby activating MdRFNR1-1 expression under drought stress. Finally, we showed that MdSUVH1 and MdDNAJ1 are positive regulators of drought tolerance. These findings illustrate the molecular roles of methylated MITE-MdRF1 (which is recognized by the MdSUVH-MdDNAJ complex) in induced MdRFNR1-1 expression as well as the drought response of apple and shed light on the molecular mechanisms of natural variation in perennial trees.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/plcell/koac220DOI Listing
July 2022

Dietary vitamins, carotenoids, and their sources in relation to age-related macular degeneration risk in China: A population-based case-control study.

Br J Nutr 2022 Jul 27:1-22. Epub 2022 Jul 27.

School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Mechanistic studies have suggested that antioxidants have beneficial effects on age-related macular degeneration (AMD). This study aimed to investigate the association between the types and sources of dietary vitamin and carotenoid intakes and AMD risk in China. A matched case-control study of 260 patients who were clinically diagnosed with AMD and 260 matched controls was performed. The participants were interviewed for dietary information and potential confounders, and comprehensive ophthalmic examinations were performed. Conditional logistic models were used to estimate the odds ratio (OR) and 95% confidence interval (CI) across the categories of specific vitamins and carotenoids and their main sources. When comparing the extreme quartiles, the ORs(95% CI) were 0.30 (0.10, 0.88) for lutein and 0.28 (0.11, 0.74) for β-cryptoxanthin. The associations between AMD and other dietary vitamin and carotenoid intakes were generally weaker and non-significant. Higher intakes of spinach and egg, which are important sources of lutein, were associated with a reduced odds of AMD, with an OR comparing the highest and lowest categories of spinach being 0.42 (95%CI: 0.20, 0.88) and that comparing the highest and lowest categories of the egg being 0.52 (95% CI: 0.27, 0.98). Participants who were in the highest category of both egg intake and spinach intake had a much greater reduced odds of having AMD (OR: 0.23; 95%CI: 0.08, 0.71) than those in the lowest category of egg intake and spinach intake. In conclusion, a higher intake of lutein and lutein-rich foods was associated with a significantly decreased odds of AMD. These findings provide further evidence of the benefits of lutein and lutein-rich foods in the prevention of AMD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S0007114522002161DOI Listing
July 2022

Stimuli-Responsive Gold Nanocages for Cancer Diagnosis and Treatment.

Pharmaceutics 2022 Jun 22;14(7). Epub 2022 Jun 22.

School of Pharmaceutical Sciences, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou 450001, China.

With advances in nanotechnology, various new drug delivery systems (DDSs) have emerged and played a key role in the diagnosis and treatment of cancers. Over the last two decades, gold nanocages (AuNCs) have been attracting considerable attention because of their outstanding properties. This review summarizes current advancements in endogenous, exogenous, and dual/multi-stimuli responsive AuNCs in drug delivery. This review focuses on the properties, clinical translation potential, and limitations of stimuli-responsive AuNCs for cancer diagnosis and treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics14071321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318695PMC
June 2022

Discrimination and Characterization of the Volatile Organic Compounds in from Six Regions of China Using HS-GC-IMS and HS-SPME-GC-MS.

Molecules 2022 Jul 8;27(14). Epub 2022 Jul 8.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Volatile organic compounds (VOCs) are the main chemical components of (SS), which have positive effects on the quality evaluation of SS. In this study, HS-SPME-GC-MS (headspace solid-phase microextraction-gas chromatography-mass spectrometry) and HS-GC-IMS (headspace-gas chromatography-ion mobility spectrometry) were performed to characterize the VOCs of SS from six different regions. A total of 82 VOCs were identified. In addition, this work compared the suitability of two instruments to distinguish SS from different habitats. The regional classification using orthogonal partial least squares discriminant analysis (OPLS-DA) shows that the HS-GC-IMS method can classify samples better than the HS-SPME-GC-MS. This study provided a reference method for identification of the SS from different origins.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules27144393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319859PMC
July 2022

A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum.

Int J Mol Sci 2022 Jul 8;23(14). Epub 2022 Jul 8.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.

Avian coronavirus-infectious bronchitis virus (AvCoV-IBV) is the causative agent of infectious bronchitis (IB) that has brought great threat and economic losses to the global poultry industry. Rapid and accurate diagnostic methods are very necessary for effective disease monitoring. At the present study, we screened a novel nanobody against IBV-N protein for development of a rapid, simple, sensitive, and specific competitive ELISA for IBV antibody detection in order to enable the assessment of inoculation effect and early warning of disease infection. Using the phage display technology and bio-panning, we obtained 7 specific nanobodies fused with horseradish peroxidase (HRP) which were expressed in culture supernatant of HEK293T cells. Out of which, the nanobody of IBV-N-Nb66-vHRP has highly binding with IBV-N protein and was easily blocked by the IBV positive serums, which was finally employed as an immunoprobe for development of the competitive ELISA (cELISA). In the newly developed cELISA, we reduce the use of enzyme-conjugated secondary antibody, and the time of whole operation process is approximately 1 h. Moreover, the IBV positive serums diluted at 1:1000 can still be detected by the developed cELISA, and it has no cross reactivity with others chicken disease serums including Newcastle disease virus, Fowl adenovirus, Avian Influenza Virus, Infectious bursal disease virus and Hepatitis E virus. The cut-off value of the established cELISA was 36%, and the coefficient of variation of intra- and inter-assay were 0.55-1.65% and 2.58-6.03%, respectively. Compared with the commercial ELISA (IDEXX kit), the agreement rate of two methods was defined as 98% and the kappa value was 0.96, indicating the developed cELISA has high consistency with the commercial ELISA. Taken together, the novel cELISA for IBV antibody detection is a simple, rapid, sensitive, and specific immunoassay, which has the potential to rapidly test IBV antibody contributing to the surveillance and control of the disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms23147589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321063PMC
July 2022

In Silico Screening and Development of Microsatellite Markers for Genetic Analysis in .

Animals (Basel) 2022 Jul 15;12(14). Epub 2022 Jul 15.

National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China.

is an economically important species of freshwater fish. To understand the genetic structure of in China, 268 samples were collected from Wulungu Lake (WL), Jili Lake (JL), the Wulungu River (WR), and the Kalaeerqisi River (KR). These samples were then analyzed using microsatellite markers. A total of 98,425 microsatellite markers were developed based on the genomic data, and 29 polymorphic microsatellite markers were selected to analyze genetic diversity in this study. The number of alleles () and observed heterozygosity () per population ranged from 4.621 (KR) to 11.172 (WL) and from 0.510 (KR) to 0.716 (JL), respectively. The results of the polymorphic information content () showed that the WL, JL, and WR populations were highly polymorphic ( 0.5) and that the KR population was moderately polymorphic (0.25 ≤ < 0.5). The genetic differentiation coefficient () among the four populations was 0.074, indicating moderate genetic differentiation among the populations in Xinjiang. The reason for the significant difference between the rivers and lakes could be the presence of a dam blocking the flow of . The development of microsatellite markers provides support for population genetics in the future. The evaluation of the genetic structure of in Xinjiang provides a reference for the reproduction and conservation of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani12141809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312242PMC
July 2022

The performance and mechanism of iron-modified aluminum sludge substrate tidal flow constructed wetlands for simultaneous nitrogen and phosphorus removal in the effluent of wastewater treatment plants.

Sci Total Environ 2022 Jul 23;847:157569. Epub 2022 Jul 23.

College of Environment, Nanjing University, Nanjing 210093, China.

Aiming at the poor N and P removal performance in the effluent of wastewater treatment plants by constructed wetlands (CWs), aluminum sludge (AS) from water supply plants was used to prepare iron-modified aluminum sludge (IAS), and tidal flow constructed wetlands (TFCWs) using IAS as substrates were constructed. By means of high-throughput sequencing, X-ray diffractometer (XRD), etc., the removal mechanism of N and P in the system and fate analysis of key elements were also interpreted. Results showed that an interlayer structure beneficial to adsorbing pollutants was formed in the IAS, due to the iron scraps entering into the molecular layers of AS. The removal rates of TP and TN by IAS-TFCWs reached 95 % and 47 %, respectively, when the flooding/resting time (F/R) and C/N were 6 h/2 h and 6. During the three-year operation of the IAS-TFCWs, the effluent concentrations of COD, NH-N, and TP could comply with Class IV Standard of "Environmental Quality Standards for Surface Water" (GB3838-2002). The mechanism analysis showed that the N removal was effectuated through Fe as the electron donor of Fe(II)-driven the autotrophic denitrifying bacteria to reduce nitrate, while the P removal mainly depended on the adsorption reaction between FeOOH in IAS and phosphate. In conclusion, the stable Fe-N cycle in the IAS-TFCWs achieved simultaneous and efficient N and P removal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.157569DOI Listing
July 2022

Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling.

Bioengineering (Basel) 2022 Jun 23;9(7). Epub 2022 Jun 23.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China.

Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) hold great potential to generate novel and curative cell therapy products. However, the current large-scale cultivation of hUCMSCs is based on empirical geometry-dependent methods, limiting the generation of high-quantity and high-quality hUCMSCs for clinical therapy. Herein, we develop a novel scale-up strategy based on computational fluid dynamics (CFD) to effectively expand the hUCMSCs in a 3D tank bioreactor. Using a standardized hUCMSCs line on microcarriers, we successfully translated and expanded the hUCMSCs from a 200 mL spinner flask to a 1.5 L computer-controlled bioreactor by matching the shear environment and suspending the microcarrier. Experimental results revealed that the batch-cultured hUCMSCs in bioreactors with an agitation speed of 40 rpm shared a more favorable growth and physiological state, similar to that run at 45 rpm in a 200 mL spinner flask, showing comparability in both culture systems. Notably, the maximum cell density reached up to 27.3 × 10 cells/mL in fed-batch culture, 2.9 folds of that of batch culture and 20.2 times of seeding cells. As such, efficient process optimization and scale-up expansion of hUCMSCs were achieved in the microcarrier-based bioreactor system by the developed CFD simulation strategy, which provided an alternative toolbox to generate massive and standardized curative cell therapy products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/bioengineering9070274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312327PMC
June 2022

Afternoon Napping Durations in Chinese Population Over 60 Years Old: Longitudinal Associations With Cognitive Performance.

Authors:
Chao Li Yan Yan

Front Public Health 2022 7;10:911498. Epub 2022 Jul 7.

Department of Epidemiology and Medical Statistics, Xiangya School of Public Health, Central South University, Changsha, China.

Introduction: Previous studies indicated inconsistent associations between daytime napping and cognitive decline. This study aimed to examine the associations between self-reported changes in napping and longitudinal cognitive performance.

Methods: A national representative sample of 4,024 participants over 60 was obtained from the China Health and Retirement Longitudinal Study from 2011 to 2018. Afternoon napping and potential factors were collected by a questionnaire. Cognitive performance was assessed on three aspects. The generalized additive models and generalized estimating equations were used to examine relationships between daytime napping and longitudinal global cognition, and generalized linear models were used to examine the longitudinal associations between change in napping at four waves and cognition in wave 4.

Results: After controlling the potential confounders, participants with afternoon napping were significantly related to better global cognition than no nappers at baseline. A change from short napping to no/long napping was associated with worse cognitive performance (β = -2.593, < 0.001). A move from no napping to short/long napping was also associated with lower cognition scores (β = -0.694, < 0.001). For participants with moderate napping, a >30 min increase (β = -1.558, < 0.001) in afternoon napping was associated with worse cognitive function in wave 4.

Conclusion: We observed that adults over 60 years old with napping <30 min per day may be at lower risk of cognitive decline. Change in napping, especially a move to extreme napping would be a risk marker underlying health conditions that impact cognition or go along with cognitive decline.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.911498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302202PMC
July 2022

Genome-Wide Analysis of and Gene Families in and Their Roles in Salt Stress Response.

Front Plant Sci 2022 7;13:918594. Epub 2022 Jul 7.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

is a halophyte with exceptional nutritional qualities, and therefore it is potentially an ideal crop to grow in saline soils, not only addressing the problem of land salinization, but also providing nutrient food for the health of humans. Currently, the molecular mechanisms underlying salt tolerance in quinoa are still largely unknown. In , receptor-like kinase (RLK1Ls) FERONIA (FER) and its ligands rapid alkalinization factors (RALFs) have been reported that participate in the regulation of salt tolerance. Here, we performed a genome-wide analysis and identified 26 and 18 family genes in quinoa genome. Transcriptomic profiling of the leaf, root, stamen, and pistil tissues of quinoa reveals that different and genes exhibit tissue-specific expression patterns, which is consistent with that observed in other plant species. RNA-seq data show that three genes are highly up-regulated after salt treatment, suggesting that some family genes are transcriptionally responsive to salt stress in quinoa. Biochemical study indicates that CqRALF15, a paralog of Arabidopsis RALF22, is physically associated with RLK1L proteins CqFER and AtFER. CqRALF15 and AtRALF22 are functionally conserved in inducing the internalization of AtFER and in triggering root growth inhibition in both quinoa and Arabidopsis. Moreover, overexpression of in Arabidopsis results in enhanced leaf bleaching under salt stress, indicating that is involved in salt stress response. Together, our study characterizes and family genes in quinoa at genomic, transcriptional, and protein levels, and provides evidence to support their roles in salt stress response.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2022.918594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302450PMC
July 2022

Introducing melatonin to the horticultural industry: physiological roles, potential applications, and challenges.

Hortic Res 2022 22;9:uhac094. Epub 2022 Apr 22.

State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.

Melatonin (-acetyl-5-methoxytryptamine) is an emerging biomolecule that influences horticultural crop growth, flowering, fruit ripening, postharvest preservation, and stress protection. It functions as a plant growth regulator, preservative and antimicrobial agent to promote seed germination, regulate root system architecture, influence flowering and pollen germination, promote fruit production, ensure postharvest preservation, and increase resistance to abiotic and biotic stresses. Here, we highlight the potential applications of melatonin in multiple aspects of horticulture, including molecular breeding, vegetative reproduction, production of virus-free plants, food safety, and horticultural crop processing. We also discuss its effects on parthenocarpy, autophagy, and arbuscular mycorrhizal symbiosis. Together, these many features contribute to the promise of melatonin for improving horticultural crop production and food safety. Effective translation of melatonin to the horticultural industry requires an understanding of the challenges associated with its uses, including the development of economically viable sources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/hr/uhac094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297156PMC
April 2022
-->