Publications by authors named "Chao Kang"

89 Publications

Randomised clinical trial: dose optimising strategy by NUDT15 genotyping reduces leucopenia during thiopurine treatment of Crohn's disease.

Aliment Pharmacol Ther 2021 Sep 25. Epub 2021 Sep 25.

Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Introduction: Thiopurine S-methyltransferase (TPTM) is a well known biomarker for thiopurine-induced leucopenia, which has limited value in Asia. Instead, NUDT15 C415T is a promising predictor in Asia.

Aims: To explore whether an optimised strategy based on NUDT15 C415T genotypes affects thiopurine-induced leucopenia, as well as efficacy in Chinese patients with Crohn's disease.

Methods: Patients with Crohn's disease and indications for thiopurines were included from two hospitals in China. They were randomly assigned to either the intervention or the control group. In the intervention group, those with genotype CC received a standard dose (control group), those with CT genotype received 50% of the standard dose, those with TT genotype received alternative drugs. The primary endpoint was thiopurine-induced leucopenia (<3.5 × 10 /L). Secondary outcomes were the incidence of other adverse events and the efficacy for maintaining steroid-free remission at week 36.

Results: The rate of thiopurine-induced leucopenia was lower in the intervention group (n = 52) than in the control group (n = 66) (23.7% vs 32.4%, P = 0.049, RR = 0.73, 95% CI 0.53-1.00). In CT subgroup, the incidence of leucopenia in the intervention group (n = 10) was significantly lower than in the control group (n = 28) (31.3% vs 65.1%, RR = 0.48, 95% CI 0.28-0.84). Neither other adverse events nor treatment efficacy was significantly different between the two groups during follow-up.

Conclusions: Among Chinese patients with Crohn's disease, dose optimisation by NUDT15 C415T reduced the rate of thiopurine-induced leucopenia, without significant influence on efficacy. Using 50% dose reduction for heterozygotes, and alternative drugs for homozygotes, are practicable strategies. Clinical trial number: NCT02929706.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/apt.16600DOI Listing
September 2021

Effects of Mobile Phone-based Telemedicine Management in Patients with Type 2 Diabetes Mellitus: A Randomized Clinical Trial.

Am J Med Sci 2021 Sep 14. Epub 2021 Sep 14.

Departments of Nutriology. Electronic address:

Background: This study aims to explore the effect of mobile phone-based telemedicine management of glycemic control of type 2 diabetes mellitus (T2DM).

Methods: Patients with T2DM were followed up in Chongqing Jiulongpo District Yuzhoulu Community Health Center, and randomly divided into the telemedicine group (n=47) and the control group (n=50). The control group received regularly routine intervention. The telemedicine management group used the mobile phone to manage their health condition remotely.

Results: Both groups had similar baseline characteristics. After a follow-up period of 12 months intervention, the weight, body mass index, waist circumference, systolic blood pressure, body fat percentage, body fat mass, body water and muscle mass, fasting blood glucose, glycosylated hemoglobin, total costs of diabetes treatment for 1 month and the quality-of-life score were significantly improved in the telemedicine group (P<0.05). And compared with the control group, body fat composition, fasting blood glucose, glycosylated hemoglobin and the cost of change shows a significant improvement (P<0.05). Positive correlation was detected between fasting blood glucose and body composition parameters, such as body fat percentage, lean body mass and body fat mass in the telemedicine group (r=0.56, P<0.05; r=0.37, P<0.05; r=0.56, P<0.05).

Conclusions: Compared with conventional intervention, the mobile phone-based telemedicine management can help patients with diabetes to improve glycemic level and quality of life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.amjms.2021.09.001DOI Listing
September 2021

Thiopurines are superior to mesalamine for preventing postoperative recurrence in patients with Crohn's disease and two or more risk factors.

J Dig Dis 2021 Aug 27. Epub 2021 Aug 27.

Department of Gastroenterology, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Objective: To confirm the hypothesis that thiopurines are better than mesalamine for preventing postoperative recurrence of Crohn's disease (CD) in patients with more than two risk factors.

Methods: In total 87 consecutive CD patients who underwent curative ileocolonic resection and ileocolic anastomosis were retrospectively recruited, including 43 prescribed with thiopurines and 44 with mesalamine after surgery. Four risk factors were predefined for subgroup analyses: smoking, penetrating disease, perianal disease and previous resection. End-points included clinical (Crohn's disease activity index >200) and endoscopic recurrence (Rutgeerts score ≥i2) within 52 weeks.

Results: There were no significant differences in clinical (37.2% vs 54.5%, P = 0.105) and endoscopic recurrence (55.8% vs 75.0%, P = 0.060) between the thiopurines and mesalamine groups by week 52. In the subgroup analysis of patients with two or more risk factors, clinical (35.7% vs 81.8%, P = 0.042) and endoscopic recurrence (64.3% vs 100%, P= 0.046) were less frequent in the thiopurine group than the mesalamine group. With one additional risk factor, the risk of endoscopic recurrence in the thiopurines group increased by 2.201-fold (95% confidence interval [CI] 1.178-4.115), adjusted for treatment intervention. While the risk of clinical and endoscopic recurrence in patients treated with mesalamine increased by 3.383-fold and 5.884-fold (95% CI 1.260-9.081 and 1.598-21.662). Three patients treated with thiopurines withdrew for adverse events.

Conclusions: Thiopurines may be superior to mesalamine for preventing postoperative recurrence of CD in patients with two or more risk factors. Caution is needed in light of the adverse events caused by thiopurines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1751-2980.13047DOI Listing
August 2021

A combined generalized Warblet transform and second order synchroextracting transform for analyzing nonstationary signals of rotating machinery.

Sci Rep 2021 Aug 20;11(1):17000. Epub 2021 Aug 20.

School of Mechanical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212013, China.

In recent years, considerable attention has been paid in time-frequency analysis (TFA) methods, which is an effective technology in processing the vibration signal of rotating machinery. However, TFA techniques are not sufficient to handle signals having a strong non-stationary characteristic. To overcome this drawback, taking short-time Fourier transform as a link, a TFA methods that using the generalized Warblet transform (GWT) in combination with the second order synchroextracting transform (SSET) is proposed in this study. Firstly, based on the GWT and SSET theories, this paper proposes a method combining the two TFA methods to improve the TFA concentration, named GWT-SSET. Secondly, the method is verified numerically with single-component and multi-component signals, respectively. Quantized indicators, Rényi entropy and mean relative error (MRE) are used to analyze the concentration of TFA and accuracy of instantly frequency (IF) estimation, respectively. Finally, the proposed method is applied to analyze nonstationary signals in variable speed. The numerical and experimental results illustrate the effectiveness of the GWT-SSET method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-96343-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379194PMC
August 2021

Modeling and prediction for diesel performance based on deep neural network combined with virtual sample.

Sci Rep 2021 Aug 18;11(1):16709. Epub 2021 Aug 18.

School of Mechanical Engineering, Jiangsu University of Science and Technology, Zhenjiang, China.

The performance models are the critical step for condition monitoring and fault diagnosis of diesel engines, and are an important bridge to describe the link between input parameters and targets. Large-scale experimental methods with higher economic costs are often adopted to construct accurate performance models. To ensure the accuracy of the model and reduce the cost of the test, a novel method for modeling the performances of marine diesel engine is proposed based on deep neural network method coupled with virtual sample generation technology. Firstly, according to the practical experience, the four parameters including speed, power, lubricating oil temperature and pressure are selected as the input factors for establishing the performance models. Besides, brake specific fuel consumption, vibration and noise are adopted to assess the status of marine diesel engine. Secondly, small sample experiments for diesel engine are performed under multiple working conditions. Moreover, the experimental sample data are diffused for obtaining valid extended data based on virtual sample generation technology. Then, the performance models are established using the deep neural network method, in which the diffusion data set is adopted to reduce the cost of testing. Finally, the accuracy of the developed model is verified through experiment, and the parametric effects on performances are discussed. The results indicate that the overall prediction accuracy is more than 93%. Moreover, power is the key factor affecting brake specific fuel consumption with a weighting of 30% of the four input factors. While speed is the key factor affecting vibration and noise with a weighting of 30% and 30.5%, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-96259-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373936PMC
August 2021

Adsorption of Hg(II) in solution by mercaptofunctionalized palygorskite.

Environ Sci Pollut Res Int 2021 Jul 31. Epub 2021 Jul 31.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, No. 9 Wenyuan Road, Yadong New District, Nanjing, 210023, China.

In the past 10 years, the treatment and restoration of soil and water bodies contaminated by mercury and other heavy metals have received unprecedented attention and support from China's environmental protection authorities. The search for low-cost and high-efficiency adsorbents has become one of the research hotspots in this field. In this paper, a simple and environment-friendly method was used to graft 3-mercaptopropyl trimethoxysilane on the surface of palygorskite. The synthesized mercaptofunctionalized palygorskite (M-PAL) was characterized by XRD, FT-IR, BET and SEM-EDS, respectively, and its adsorption conditions, adsorption models and thermodynamic parameters for Hg were systematically investigated. The experimental results indicated that the saturated adsorption capacity of Hg on the M-PAL could reach 203.4 mg·g, within 120 min at pH 4 and 298 K. By analyzing the experimental data of adsorption kinetics and thermodynamics, it was found that the adsorption process of Hg conformed to the pseudo-second-order kinetic model, which belonged to chemical adsorption of the rate-controlled step; the Langmuir model better described the adsorption isotherm. The thermodynamic parameters obtained (ΔH=29.95 kJ·mol, ΔS=103.09 J·mol·K and ΔG<0) show that the whole process is a spontaneous endothermic process. When the concentration of Na, K, Ca, Mg, Cl, NO, HCO and CHO was 200 times that of Hg, although these organic acids had a slightly greater effect on the adsorption of Hg on mercaptofunctionalized palygorskite than inorganic ions, the adsorption capacity remained above 185 mg·g. The adsorption products could be still stable in simulated acid rain with pH 3, 4, 5, 6, 7 and oxalic acid solution with concentration of 1, 2, 3, 4 and 5 mmol·L, and the desorption rates were about 3%. Through XPS analysis, the specific coordination of Hg with the S atom on the surface of M-PAL was confirmed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15637-0DOI Listing
July 2021

Unexpected Liver Lesions in Crohn's Disease.

Inflamm Bowel Dis 2021 Jul 28. Epub 2021 Jul 28.

Department of Gastroenterology, the Sixth Affiliated Hospital, Sun Yat-sen University, People's Republic of China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/ibd/izab186DOI Listing
July 2021

Binary Surfactant-Mediated Tunable Nanotip Growth on Gold Nanoparticles and Applications in Photothermal Catalysis.

Front Chem 2021 7;9:699548. Epub 2021 Jul 7.

School of Physics and Information Technology, Shaanxi Normal University, Xi'an, China.

Plasmonic nanostructures with sharp tips are widely used for optical signal enhancement because of their strong light-confining abilities. These structures have a wide range of potential applications, for example, in sensing, bioimaging, and surface-enhanced Raman scattering. Au nanoparticles, which are important plasmonic materials with high photothermal conversion efficiencies in the visible to near-infrared region, have contributed greatly to the development of photothermal catalysis. However, the existing methods for synthesizing nanostructures with tips need the assistance of poly(vinylpyrrolidone), thiols, or biomolecules. This greatly hinders signal detection because of stubborn residues. Here, we propose an efficient binary surfactant-mediated method for controlling nanotip growth on Au nanoparticle surfaces. This avoids the effects of surfactants and can be used with other Au nanostructures. The Au architecture tip growth process can be controlled well by adjusting the ratio of hexadecyltrimethylammonium bromide to hexadecyltrimethylammonium chloride. This is due to the different levels of attraction between Br/Cl and Au ions. The surface-enhanced Raman scattering and catalytic abilities of the synthesized nanoparticles with tips were evaluated by electromagnetic simulation and photothermal catalysis experiments (with 4-nitrothiophenol). The results show good potential for use in surface-enhanced Raman scattering applications. This method provides a new strategy for designing plasmonic photothermal nanostructures for chemical and biological applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.699548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294035PMC
July 2021

Twelve-week peptide-based formula therapy may be effective in inducing remission of active Crohn disease among women who are pregnant or preparing for pregnancy.

Nutr Clin Pract 2021 Jul 10. Epub 2021 Jul 10.

Department of Gastroenterology, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.

Background: Conventional treatment for Crohn disease (CD) in pregnancy includes mesalamine, thiopurine, and anti-tumor necrosis factor (TNF)-α agents. However, women may abstain because of complications, nonresponse, or potential adverse outcomes. Peptide-based formula therapy, through oral or nasogastric feeding without other food intake, is an effective and safe therapy for active CD. Herein, We confirmed the effectiveness and safety of peptide-based formula therapy for active CD in pregnant women or those preparing for pregnancy.

Method: Outcomes of peptide-based formula therapy to induce CD remission during pregnancy preparation and the conception period were evaluated retrospectively among 14 women. Efficacy was evaluated as the change in serum indices and inflammatory markers after 12-week treatment. Pregnancy outcomes were compared between 14 women treated with nutrition therapy and eight women using conventional CD drugs.

Results: After 12 weeks, 85.7% (12 of 14) of patients treated with peptide-based formula achieved remission with a significant decrease in the CD activity index (P < .001) and high-sensitivity C-reactive protein level (P = .004). There were no effects of peptide-based formula therapy on pregnancy outcomes compared with conventional CD treatment (P > .05). Among the 12 patients who achieved CD remission with exclusive peptide-based formula therapy, 10 selected to continue total or partial peptide-based formula treatment to maintain CD remission throughout pregnancy.

Conclusion: Peptide-based formula therapy, without other food intake, may provide a safe and effective alternative to conventional CD drugs to induce disease remission among women during conception and pregnancy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ncp.10733DOI Listing
July 2021

Synthesis of OmpA Oral Nanoparticles and Evaluation of Immune Functions against the Major Etiologic Agent of Cow Mastitis.

Vaccines (Basel) 2021 Mar 23;9(3). Epub 2021 Mar 23.

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

is a major etiologic agent of cow mastitis, a condition that results in huge economic losses. There is a lack of an oral vaccine for cow mastitis. Previous studies have confirmed that the outer membrane protein A (OmpA) of is immunogenic and can be used for vaccine design. In the present study, OmpA was encapsulated into nanoparticles (NP-OmpA) for an oral vaccine for cow mastitis. OmpA was purified with Ni-NTA flow resin and encapsulated with chitosan (CS) to prepare NP-OmpA nanoparticles. The gastrointestinal tract was simulated in vitro (PBS, pH 1.2) to measure the protein release rate. The optimal preparation conditions for NP-OmpA were determined by analyzing the concentrations of OmpA and CS, magnetic mixing speed, mixing time, and the ratio of tripolyphosphate (TPP)/CS (). NP-OmpA safety was assessed by function factors and histopathological examination of livers and kidneys. The immune activity of NP-OmpA was determined using qRT-PCR to assess immune-related gene expression, leukocyte phagocytosis of , ELISA to evaluate antiserum titer and immune recognition of , and the organ index. The immune protection function of NP-OmpA was assessed by the protection rate of NP-OmpA to in mice, qRT-PCR for inflammation-related gene expression, assay kits for antioxidant factors, and visceral injury in the histopathological sections. NP-OmpA nanoparticles had a diameter of about 700 nm, loading efficiency (LE) of 79.27%, and loading capacity (LC) of 20.31%. The release rate of NP-OmpA (0~96 h) was less than 50% in vitro. The optimal preparation conditions for NP-OmpAs were OmpA protein concentration of 2 mg/mL, CS concentration of 5 mg/mL, TPP/CS () of 1:1, magnetic mixing speed of 150 r/min, and mixing time of 15 min. Histopathological sections and clinical analytes of uric acid (UA), creatinine (Cr), alanine aminotransferase (ALT), aspartate transaminase (AST), catalase (CAT), glutathione (GSH), and malondialdehyde (MDA) showed NP-OmpA did not damage mice livers or kidneys. NP-OmpA could enhance the immune-related gene expression of IFN-γ and HSP70 in the spleen, liver, and kidney and the leukocyte phagocytosis of . The antiserum titer (1:3200) was obtained from mice immunized with NP-OmpA, which had an immune recognition effect to . The immune protection rate of NP-OmpA was 71.43% ( < 0.05) to . NP-OmpA could down-regulate the inflammation-related gene expression of TNF-a, IL-6, and IL-10 in the spleen, liver, and kidney, and the antioxidant factors MDA and SOD in the liver, and reduce injury in the liver and kidney of mice induced by . A novel NP-OmpA nanoparticle was encapsulated, and the optimal preparation conditions were determined. The NP-OmpA was safe and had good immune functions. They are expected to induce a response that resists infection with the major etiologic agent () of cow mastitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/vaccines9030304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005184PMC
March 2021

Platelet-to-lymphocyte percentage ratio index: a simple non-invasive index to monitor the endoscopic activity in Crohn's disease.

Therap Adv Gastroenterol 2020 21;13:1756284820979442. Epub 2020 Dec 21.

Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan Road 2, Guangzhou 510080, P.R. China.

Background: Recent evidence has shown that the complete blood count (CBC) is abnormal in patients with Crohn's disease (CD). We aimed to investigate an effective CBC parameter and explore its impact on disease activity in a large CD cohort.

Methods: We performed a retrospective analysis of patients with established CD who underwent clinically indicated endoscopy at four tertiary centres in China between 2016 and 2020. Individual variables of the Simple Endoscopic Score for CD, CBC parameters, C-reactive protein (CRP) levels, erythrocyte sedimentation rate, and faecal calprotectin (FC) were independently reviewed by different investigators. The hold-out method was used to verify the predictive power of the established model.

Results: Data from a total of 1388 endoscopic procedures performed for 882 eligible CD patients were available with routine blood parameters and related indicators. The model using platelet-to-lymphocyte percentage ratio (PLpR) had high accuracy for identifying patients in endoscopic remission (ER), with an area under the curve (AUC) of 0.785 [95% confidence interval (CI): 0.784-0.787], which was comparable with that for CRP (AUC: 0.775, 95% CI: 0.774-0.777). Notably, the AUC of PLpR was significantly higher than that of CRP in patients with colonic disease and with a history of surgery. Moreover, after combining the FC with PLpR, the AUC value of FC + PLpR increased up to 0.892 (95% CI: 0.890-0.894) for identifying ER.

Conclusions: We explored an index (PLpR) to identify CD patients in ER based on platelet and lymphocyte percentage from the CBC. PLpR helped evaluate the degree of disease activity and monitor the therapeutic response.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1756284820979442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758565PMC
December 2020

A Review of Carbon Dots Produced from Biomass Wastes.

Nanomaterials (Basel) 2020 Nov 23;10(11). Epub 2020 Nov 23.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

The fluorescent carbon dot is a novel type of carbon nanomaterial. In comparison with semiconductor quantum dots and fluorescence organic agents, it possesses significant advantages such as excellent photostability and biocompatibility, low cytotoxicity and easy surface functionalization, which endow it a wide application prospect in fields of bioimaging, chemical sensing, environmental monitoring, disease diagnosis and photocatalysis as well. Biomass waste is a good choice for the production of carbon dots owing to its abundance, wide availability, eco-friendly nature and a source of low cost renewable raw materials such as cellulose, hemicellulose, lignin, carbohydrates and proteins, etc. This paper reviews the main sources of biomass waste, the feasibility and superiority of adopting biomass waste as a carbon source for the synthesis of carbon dots, the synthetic approaches of carbon dots from biomass waste and their applications. The advantages and deficiencies of carbon dots from biomass waste and the major influencing factors on their photoluminescence characteristics are summarized and discussed. The challenges and perspectives in the synthesis of carbon dots from biomass wastes are also briefly outlined.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano10112316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700468PMC
November 2020

Deep Learning Correction Algorithm for The Active Optics System.

Sensors (Basel) 2020 Nov 9;20(21). Epub 2020 Nov 9.

CAS Nanjing Astronomical Instruments Co., Ltd., Nanjing 210042, China.

The correction of wavefront aberration plays a vital role in active optics. The traditional correction algorithms based on the deformation of the mirror cannot effectively deal with disturbances in the real system. In this study, a new algorithm called deep learning correction algorithm (DLCA) is proposed to compensate for wavefront aberrations and improve the correction capability. The DLCA consists of an actor network and a strategy unit. The actor network is utilized to establish the mapping of active optics systems with disturbances and provide a search basis for the strategy unit, which can increase the search speed; The strategy unit is used to optimize the correction force, which can improve the accuracy of the DLCA. Notably, a heuristic search algorithm is applied to reduce the search time in the strategy unit. The simulation results show that the DLCA can effectively improve correction capability and has good adaptability. Compared with the least square algorithm (LSA), the algorithm we proposed has better performance, indicating that the DLCA is more accurate and can be used in active optics. Moreover, the proposed approach can provide a new idea for further research of active optics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20216403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665141PMC
November 2020

Early serum infliximab trough level and mucosal healing could be predictors for one-year outcome after initiating therapy in Crohn's disease.

Scand J Gastroenterol 2020 Sep 20;55(9):1035-1040. Epub 2020 Aug 20.

Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.

Background And Aims: Serum infliximab trough level(S-IFX) and antibody were documented to correlate with clinical response. The aim of this study was to identify the relationship between early S-IFX, early mucosal healing (MH) and one-year outcome in a cohort on maintenance IFX therapy in Crohn's disease (CD).

Methods: The study group comprised of retrospectively enrolled patients diagnosed for Crohn's disease ( = 108). Patients received scheduled maintenance therapy after response to IFX induction, and had undergone the early S-IFX test and endoscopic examination at week 14. Clinical outcomes were evaluated during maintenance therapy until week 52.

Results: Early S-IFX was 4.78 ± 6.16 ug/ml in all the patients and 19% (21/108) of them developed antibodies, and 52 patients reached early MH. During 52 weeks' follow-up. Twenty-eight percent (30/108) of patients showed loss of response to IFX. Patients who lost response had lower early S-IFX than those who had sustained response (3.01 ± 3.66  5.47 ± 6.79 ug/ml,  = .02; 48 23%,  =  .02). At week 52, 73 patients had repeated endoscopy and 42% of them reached MH. Early S-IFX had a predictive value on MH at week 52. When early S-IFX > 2.5 ug/ml, the sensitivity for predicting MH at week 52 was 87%, and the specificity were 61% (AUC = 0.73,  < .01). The combined predictive value of early S-IFX and early MH became stronger. Only 6% (1/18) of those patients who had low early S-IFX and had not reached early MH could reach MH at week 52.

Conclusions: Early S-IFX and early MH could predict one-year response after initiating IFX therapy in Crohn's disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00365521.2020.1800077DOI Listing
September 2020

Enhancing the selectivity of liquid chromatography-mass spectrometry by using trilinear decomposition on LC-MS data: An application to three-way calibration of coeluting analytes in human plasma.

J Sep Sci 2020 Jul 23;43(13):2718-2727. Epub 2020 Apr 23.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, P. R. China.

The high selectivities of liquid chromatography and mass spectrometry make liquid chromatography-mass spectrometry one of the most popular tools for quantitative analysis in complex chemical, biological, and environmental systems, while the potential mathematical selectivity of liquid chromatography-mass spectrometry is rarely investigated. This work discussed the mathematical selectivity of liquid chromatography-mass spectrometry by three-way calibration based on the trilinear model, with an application to quantitative analysis of coeluting aromatic amino acids in human plasma. By the trilinear decomposition of the constructed liquid chromatography-mass spectrometry-sample trilinear model and individual regression of the decomposed relative intensity versus concentration, the proposed three-way calibration method successfully achieved quantitative analysis of coeluting aromatic amino acids in human plasma, even in the presence of uncalibrated interferent(s) and a varying background. This analytical method can ease the requirements for sample preparation and complete chromatographic separation of components, reduce the use of organic solvents, decrease the time of chromatographic separation, and increase the peak capacity of liquid chromatography-mass spectrometry. As a "green analytical method", the liquid chromatography-mass spectrometry three-way calibration method can provide a promising tool for direct and fast quantitative analysis in complex systems containing uncalibrated spectral interferents, especially for the situation where the coelution problem is difficult to overcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.202000151DOI Listing
July 2020

Evaluation of Immunogenicity, Protective Immunity on Aquaculture Pathogenic and Fermentation of Flagellin FlaC Protein.

Iran J Biotechnol 2019 Sep 1;17(3):e2628. Epub 2019 Sep 1.

Chinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.

Background: are the main pathogenic bacteria in aquaculture. The flagellin protein C (FlaC) of has good immunogenicity and the prospect of potential application in a vaccine.

Objectives: We aimed to evaluate the immunogenicity, protective immunity, and prokaryotic expression fermentation of FlaC protein for the vaccine in aquaculture.

Material And Methods: A molecular cloning method was used to construct the expression strain of FlaC protein, and the protein was purified with Ni-affinity chromatography. Polyclonal antiserum was prepared via mice immunized with the FlaC protein. The Western blot and enzyme-linked immunosorbent assay (ELISA) were used to check the specificity and titre of the antiserum. ELISA and pull-down assay detected the interaction between FlaC protein antiserum and . The immune protection function of FlaC protein was detected with mice actively immunized with FlaC protein and challenged by and . The optimal expression conditions for FlaC protein were detected using an L(3) orthogonal design model.

Results: The expression strain of FlaC protein was obtained successfully, and purified FlaC protein was prepared using a mice polyclonal antibody. The FlaC protein antiserum held a high specificity, and the titre was 13200. The antiserum directly interacted with and , and the FlaC protein demonstrated a significant immune protection function (50%) against infection and some immune protection function (41.66%) against . The optimal expression conditions for FlaC protein included a strain OD value of 0.8, final isopropyl-β-d-thiogalactoside (IPTG) concentration of 0.1 mmol/L, an inducing time of 8 hours, and an inducing temperature of 28°C.

Conclusions: This study showed that the FlaC protein possesses a significant immunogenicity and immune protection effect and obtained the optimal fermentation conditions. It is expected to be a potential vaccine against and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.29252/ijb.2628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080974PMC
September 2019

UPLC-QTOF-MS-Based Plasma Lipidomic Profiling Reveals Biomarkers for Inflammatory Bowel Disease Diagnosis.

J Proteome Res 2020 02 26;19(2):600-609. Epub 2019 Dec 26.

Institute of Clinical Pharmacology, School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou 510006 , P. R. China.

Identification of new biomarkers may help in the early diagnosis of inflammatory bowel disease (IBD). In this study, ultrahigh-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used to analyze the untargeted lipidomics and compare plasma lipid profiles between IBD patients and control subjects. The principal component analysis and partial least-squares-discriminant analysis were carried out to distinguish IBD patients from control subjects. Using univariate and multivariate analysis, 55 significantly different metabolites from five lipid classes, fatty acyls ( = 19), glycerophospholipids ( = 5), prenol lipids ( = 10), sphingolipids ( = 2), and sterol lipids ( = 19) were identified. Forty-four of the 55 metabolites were analyzed by receiver operating characteristic (ROC) curve and area under curve (AUC) of >0.80. After validation in an independent cohort, IBD patients were differentiated from the control subjects by significantly altered plasma level of palmitic acid, 7alpha, 25-dihydroxycholesterol, 20-hydroxyeicosatetraenoic (HETE)-d6, (+/-)5,6-epoxy-eicosatrienoic acid (EpETrE), docosahexaenoic acid (DHA), 9-heptadecylenic acid, lactucaxanthin, α-carotene, traumatic acid, and neoquassin with both sensitivity and specificity above 80%. Pathway analysis suggested that IBD dysregulation was related to the biosynthesis of primary bile acid, the metabolism of arachidonic acid, the metabolism of sphingolipid, fatty acid elongation, and glycerophospholipid metabolism. Our results suggest that the lipidomic profiling of patients plasma could be a potential method for IBD diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jproteome.9b00440DOI Listing
February 2020

High-risk human papilloma virus infection and cervical neoplasm in female inflammatory bowel disease patients: a cross-sectional study.

Gastroenterol Rep (Oxf) 2019 Oct 15;7(5):338-344. Epub 2019 Feb 15.

Department of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Background And Aim: This cross-sectional study investigated the prevalence and risk factors of high-risk human papilloma virus (HPV) infection, especially types 16 and 18, and cervical neoplasia in female Inflammatory bowel disease (IBD) patients.

Methods: From July 2014 to January 2017, sexually active, female, Chinese IBD patients (21-60 years) and age-matched controls underwent cervical ThinPrep cytology testing (TCT) and high-risk HPV-DNA detection, and completed questionnaires about awareness of cervical cancer and HPV. Cervical dysplasia was categorized as cervical intraepithelial neoplasia (CIN) 1, 2 and 3.

Results: Of 124 IBD patients (30 ulcerative colitis and 94 Crohn's disease), 17 (13.7%) had high-risk HPV among whom 9 (7.3%) had HPV 16/18 infection and 4 (3.2%) had cervical CIN (3 CIN 3, 1 CIN 1) by pathology. Among 372 controls, 33 (8.9%) had high-risk HPV and only 1 (0.3%) had HPV 16 infection. Cervical TCT detected atypical squamous cells of unknown significance in one control; no control had CIN. The HPV 16/18 infection rate and CIN prevalence were significantly higher in IBD patients than controls (both  < 0.001). The HPV-infection rate was higher in patients administered methotrexate [ = 0.005, odds ratio (95% confidence interval) 4.76 (1.471-15.402)] or more than two immunosuppressants [ = 0.013, odds ratio (95% confidence interval) 3.64 (1.255-10.562)]. Thiopurine, steroid, infliximab and disease behavior/location were not associated with HPV infection. Only 29.3% of patients had undergone cervical-cancer screening. Awareness of HPV infection and HPV-related cervical cancer was poor (28.2%).

Conclusions: Female IBD patients are at increased risk of high-risk HPV infection and cervical neoplasia, which may be associated with immunosuppressants. Education and routine follow-up with HPV-DNA testing and TCT are recommended, especially in female Chinese IBD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/gastro/goy053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821219PMC
October 2019

Nucleoside diphosphate-linked moiety X-type motif 15 R139C genotypes impact 6-thioguanine nucleotide cut-off levels to predict thiopurine-induced leukopenia in Crohn's disease patients.

World J Gastroenterol 2019 Oct;25(38):5850-5861

Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China.

Background: Thiopurine-induced leukopenia (TIL) is a life-threatening toxicity and occurs with a high frequency in the Asian population. Although nucleoside diphosphate-linked moiety X-type motif 15 () variants significantly improve the predictive sensitivity of TIL, more than 50% of cases of this toxicity cannot be predicted by this mutation. The potential use of the 6-thioguanine nucleotide (6TGN) level to predict TIL has been explored, but no decisive conclusion has been reached. Can we increase the predictive sensitivity based on 6TGN by subgrouping patients according to their R139C genotypes?

Aim: To determine the 6TGN cut-off levels after dividing patients into subgroups according to their R139C genotypes.

Methods: Patients' clinical and epidemiological characteristics were collected from medical records from July 2014 to February 2017. R139C, thiopurine S-methyltransferase, and 6TGN concentrations were measured.

Results: A total of 411 Crohn's disease patients were included. TIL was observed in 72 individuals with a median 6TGN level of 323.4 pmol/8 × 10 red blood cells (RBC), which was not different from that of patients without TIL ( 0.071). Then, we compared the 6TGN levels based on R139C. For CC ( = 342) and CT ( = 65) genotypes, the median 6TGN level in patients with TIL was significantly higher than that in patients without (474.8 306.0 pmol/8 × 10 RBC, = 9.4 × 10; 291.7 217.6 pmol/8 × 10 RBC, = 0.039, respectively). The four TT carriers developed TIL, with a median 6TGN concentration of 135.8 pmol/8 × 10 RBC. The 6TGN cut-off levels were 411.5 and 319.2 pmol/8 × 10 RBC for the CC and CT groups, respectively.

Conclusion: The predictive sensitivity of TIL based on 6TGN is dramatically increased after subgrouping according to R139C genotypes. Applying 6TGN cut-off levels to adjust thiopurine therapies based on is strongly recommended.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3748/wjg.v25.i38.5850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801191PMC
October 2019

Removal of Fluorine from Wet-Process Phosphoric Acid Using a Solvent Extraction Technique with Tributyl Phosphate and Silicon Oil.

ACS Omega 2019 Jul 3;4(7):11593-11601. Epub 2019 Jul 3.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

The deep removal of fluorine from wet-process phosphoric acid is currently a very serious issue. In this paper, an efficient liquid-liquid separation method based on a bubble membrane was developed to solve this problem. Tributyl phosphate (TBP) and silicon oil (SIO) were used as the organic phase. The effects of the component proportion in the organic phase (TBP/SIO v/v), organic to aqueous phase ratio (O/A), pH, temperature, and reaction time on the extraction ratio were investigated. The extraction ratio of fluorine was 98.4% when using only one stage with the following conditions: 90 °C, pH -0.46, volume ratio (TBP/SIO v/v) of 7:3, phase ratio (O/A) of 1:5, stirring speed of 200 rpm, and reaction time of 50 min. Fourier-transform infrared spectroscopy and inverted fluorescence microscopy were used to investigate the reaction mechanism and reaction kinetics. In addition, the scrubbing and stripping process was investigated. When a 2 mol/L sodium hydroxide solution ([NaOH]) was used as the stripping agent with a phase ratio (O/A) of 1:10, a stirring speed of 200 rpm, and a reaction time of 30 min, a maximum stripping ratio of 90.1% was obtained.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b01383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682134PMC
July 2019

Radioiodine hot feet sign on post-ablation high dose of I of whole-body scan.

Hell J Nucl Med 2019 Jan-Apr;22(1):77

Department of Nuclear Medicine, Chung-Kang branch, Cheng-Ching General Hospital, Taichung, Taiwan.

View Article and Find Full Text PDF

Download full-text PDF

Source
August 2019

Synergy between dihydromyricetin intervention and irinotecan chemotherapy delays the progression of colon cancer in mouse models.

Food Funct 2019 Apr;10(4):2040-2049

Research Center for Nutrition and Food Safety, Institute of Military Preventive Medicine, Army Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Medical Nutrition Research Center, Chongqing, P.R. China.

Colorectal cancer (CRC) is the third highest cause of cancer-related death and the main option for prolonged survival is chemotherapeutic intervention. There is increasing interest in dietary intervention using natural agents to enhance the sensitivity of such invasive chemical treatment. In this study, the chemotherapeutic efficacy of dihydromyricetin (DMY) intervention on treatments involving irinotecan (CPT-11) or gemcitabine (GM) was evaluated in an AOM/DSS-induced colitis-associated colon cancer model and a Min (Apc Min/+) mice model. Our data showed that DMY could promote the CPT-11 effect both in the mouse model of AOM/DSS and Apc Min/+ cancer and had no influence on the GM effect. In AOM/DSS cancer, tumors were sensitive to 100 mg kg-1 DMY chemotherapy under 100 mg kg-1 or 200 mg kg-1 CPT-11. DMY-driven CPT-11 chemotherapy induced enhanced IgG levels and the reduction of Fusobacterium abundance in the gut. In the Min model, CPT-11 with 20 mg kg-1 DMY prevented tumor formation but not with 100 mg kg-1 DMY. Mechanically, chloride ion-dependent CFTR, CLCN4, and CLIC4 signaling are not involved in DMY mediated chemotherapeutic colon tumorigenesis. These results suggested that a suitable dose of DMY could act as a coadjuvant to CPT-11 chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8fo01756eDOI Listing
April 2019

Relationship of common variants in Interleukin 33 gene with susceptibility and prognosis of osteosarcoma in Han Chinese population.

J Cancer 2019 29;10(5):1138-1144. Epub 2019 Jan 29.

Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.

Osteosarcoma (OS) is one of the most common malignant bone tumors. Many previous studies have indicated that OS is a complex disease and that its development may be affected by multiple genetic factors, which may contribute to its carcinogenesis. The aim of the present study was to evaluate the relationship of with susceptibility and prognosis of OS in Han Chinese individuals. A total of 1,605 study subjects including 507 OS patients and 1,098 controls were recruited. Eighteen SNPs mapped to were selected for genotyping. Genetic associations between selected SNPs and OS disease status were evaluated. Survival analyses, including Kaplan-Meier analysis and Cox model fitting for significant SNPs, were performed. The functional consequences of significant SNPs were analyzed using a publicly available database. SNP rs1048274 was identified to be significantly associated with OS disease status (OR=0.75, =1.53×10). Compared to the GA and GG groups, OS patients with the AA genotype of rs1048274 had better survival rate. The hazard ratio of SNP rs1048274 (AA group compared to GG+GA group) was 0.35 (95% confidence interval of 0.25-0.5) following adjustment for several clinical variables. In conclusion, our results suggested that may play a key role in the etiology of OS, indicating as a potential genetic risk factor of the development and prognosis of OS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.29086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400670PMC
January 2019

Simultaneously quantifying intracellular FAD and FMN using a novel strategy of intrinsic fluorescence four-way calibration.

Talanta 2019 May 26;197:105-112. Epub 2018 Dec 26.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China. Electronic address:

The simultaneous quantitative analysis of intracellular metabolic coenzymes flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) is of interest because they participate in many electron-transfer reactions of metabolism. But, the simultaneous quantitative analysis of FAD and FMN is hard to be achieved by traditional analytical methods. This paper proposes a novel strategy of intrinsic fluorescence coupled with four-way calibration method for simultaneous quantitative analysis of intracellular metabolic coenzymes FAD and FMN. Through mathematical separation, this proposed analytical method efficiently achieved the simultaneous quantitative analysis of metabolic coenzymes FAD and FMN in the cell, despite the fact that uncalibrated spectral interferents coexist in the system. The predicted concentrations of FAD and FMN in the cell are 217.0 ± 6.9 and 155.0 ± 1.7 pmol/10 cells respectively, which were validated by the approved liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. This analytical method with second-order advantage simply requires the cell solution to be diluted by a buffer, it could introduce an interesting analytical strategy for multianalyte direct quantitative analysis in complex biological systems. In addition, we explore the third-order advantage of four-way calibration by a comparative study based on this real fluorescence data. The comparisons indicate that a four-way calibration method can provide higher sensitivity and more resolving power than a three-way calibration method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2018.12.076DOI Listing
May 2019

Circulating microRNA146b-5p is superior to C-reactive protein as a novel biomarker for monitoring inflammatory bowel disease.

Aliment Pharmacol Ther 2019 03 7;49(6):733-743. Epub 2019 Feb 7.

Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. China.

Background: Owing to the importance of early treatment, simple and reliable methods for monitoring inflammatory bowel disease (IBD) are needed.

Aims: To determine whether circulating microRNAs are reliable biomarkers for IBD monitoring.

Methods: Serum levels of 17 candidate microRNAs were measured by quantitative real-time polymerase chain reaction in a discovery cohort (n = 120). Differentially expressed serum microRNAs were further investigated in an independent training cohort (n = 341). Correlations between relative microRNA levels and disease activity were evaluated. A disease control group was included to investigate the specificity of microRNA. Logistical regression was used to construct a microRNA classifier to identify endoscopic activity. Its predictive value was explored in the validation cohort (n = 66) using the area under the receiver operating characteristic curve (AUC).

Results: Serum microRNA146b-5p (miR-146b-5p) expression was 2.87- and 2.72-fold higher in patients with Crohn's disease and ulcerative colitis, respectively, than in healthy controls. Serum miR-146b-5p was significantly correlated with disease activity and was more specific than C-reactive protein (CRP). A classifier was built for Crohn's disease, ie P [Endoscopically active] = , with a greater AUC of 0.869 [0.764-0.940] than that for CRP (0.680 [0.554-0.790]) (P = 0.0043).

Conclusions: MiR-146b-5p may better reflect mucosal inflammation in IBD than CRP. The Crohn's disease classifier developed in this study may be valuable for identifying endoscopic activity in patients with Crohn's disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/apt.15159DOI Listing
March 2019

Correction to: Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells.

Stem Cell Res Ther 2019 Jan 21;10(1):33. Epub 2019 Jan 21.

Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.

The original article [1] contains a duplication error within Figure 3.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-019-1132-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341599PMC
January 2019

Theoretical study on polynitro imidazo [4, 5-e] oxadiazolo [3, 4-b] pyrazine compounds.

J Mol Model 2019 Jan 5;25(1):25. Epub 2019 Jan 5.

State Key Laboratory of Fluorine & Nitrogen Chemicals, Xi'an Modern Chemistry Research Institute, Xi'an, 710065, China.

The B3PW91/6-31G** theoretical method was carried out to optimize the structure of 12 polynitro imidazo [4,5-e] oxadiazolo [3,4-b] pyrazine compounds (two structural type). The influence of nitro groups on the structure, oxygen balance, density, heat of formation, detonation performances, and charge were investigated. The results showed that the oxygen balance, density, heat of formation, detonation velocity, detonation pressure, and detonation heat increased with different relationships when the number of nitro groups increased. The contribution of the dinitroethylene group to energy was greater than that of the nitroimino group. On the whole, the sensitivity of all compounds increased with the number of -NO groups, and the second type of compound is more sensitive because of more nitro groups. The alkaline of the amine will decrease with the increasing number of -NO groups, and nitrification action will become more difficult. Graphical abstract Polynitro imidazo [4, 5-e] oxadiazolo [3, 4-b] pyrazine compoundsᅟ.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00894-018-3903-5DOI Listing
January 2019

Angle Distribution of Loading Subspace (ADLS) for estimating chemical rank in multivariate analysis: Applications in spectroscopy and chromatography.

Talanta 2019 Mar 10;194:90-97. Epub 2018 Oct 10.

LASIR CNRS UMR 8516, Université de Lille, Sciences et Technologies, 59655 Villeneuve d'Ascq Cedex, France. Electronic address:

Multivariate analyses are increasingly popular to explore the underlying structure of multivariate datasets, which are more and more prevalent in analytical chemistry. However, difficulties can be associated with estimating the number of components for the data with considerable coherence and noise. The method of Angle Distribution of Loading Subspace (ADLS) has been proposed to estimate the number of components for Principal Component Analysis (PCA) and PARAllel FACtor analysis (PARAFAC), which showed some advantages, in particular in the case of datasets with high coherence, over the commonly used methods (scree plot and cross-validation in PCA, and core consistency diagnostics (CORCONDIA) in PARAFAC). In this paper, we systematically improved and applied ADLS to estimate the number of components in different multivariate methods including, Multivariate Curve Resolution (MCR), PARAFAC and four-way PARAFAC. Firstly, we showed that ADLS performed better when estimating the chemical rank for MCR analysis, compared with scree plots. As well as this, we improved ADLS in multi-way analysis (three- and four-way PARAFAC) by calculating the loading subspace in advance using the Khatri-Rao product. The improved ADLS in multi-way analysis provided the correct result for the simulated three-way fluorescence datasets with unevenly distributed coherence at different dimensions, while the previous version of ADLS showed biased results and CORCONDIA / split-half analysis provided relatively unstable results. Moreover, ADLS was used to estimate the chemical rank for a four-way real-life fluorescence dataset analyzed by four-way PARAFAC. In this case the result of chemical rank results from ADLS was more precise and informative compared with CORCONDIA /split-half analysis in four-way analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2018.10.008DOI Listing
March 2019

SIRT3 Deficiency Promotes High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease in Correlation with Impaired Intestinal Permeability through Gut Microbial Dysbiosis.

Mol Nutr Food Res 2019 02 27;63(4):e1800612. Epub 2018 Dec 27.

Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, P. R. China.

Scope: Sirtuin 3 (SIRT3) plays a protective role against nonalcoholic fatty liver disease (NAFLD) by improving hepatic mitochondrial dysfunction. Gut microbiota imbalance contributes to the pathogenesis of NAFLD, yet the underlying mechanism linking SIRT3 with gut microbiota in NAFLD progression remains obscure.

Methods And Results: Wild-type 129 mice and SIRT3 knockout (SIRT3KO) mice are placed under a chow diet or high-fat diet (HFD) treatment for 18 weeks. HFD resulted in a significantly increased hepatic steatosis and inflammation, which are exacerbated in SIRT3KO mice. The gut microbiota by 16s rRNA gene sequencing and phylogenetic reconstruction of unobserved states analysis are characterized. Lack of SIRT3 facilitates gut microbial dysbiosis in mice following HFD, with increased Desulfovibrio, Oscillibacter, and decreased Alloprevotella. SIRT3 deficiency resulted in an impaired intestinal permeability and inflammation in HFD-fed mice, which can be attenuated by sodium butyrate (NaB). SIRT3KO HFD-fed mice is followed by an increased lipopolysaccharide into the circulation and dysregulated expressions of cannabinoid receptor 1 and 2 in colon and liver, which are significantly associated with the alterations of intestinal microbiota.

Conclusions: SIRT3 deficiency promotes NAFLD progression in correlation with impaired intestinal permeability through gut microbiota dysbiosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mnfr.201800612DOI Listing
February 2019

Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae.

Vaccine 2018 12 13;36(52):8079-8083. Epub 2018 Nov 13.

Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. Electronic address:

Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Bacterial surface proteins are promising vaccine candidates. We recently identified 3 E. rhusiopathiae surface proteins (GAPDH, HP0728, and HP1472) and characterized their roles as virulence factors. However, their efficacy as protective antigens is still unknown. The N-terminal region of a previously identified surface protein, CbpB (CbpB-N), is speculated to be a protective antigen, but this needs to be verified. The aim of this study was to evaluate the protective efficacy of GAPDH, HP0728, HP1472, and CbpB-N. Immunization with recombinant GAPDH provided complete protection in a mouse model, recombinant CbpB-N provided partial protection, while recombinant HP0728 and HP1472 provided no protection. Recombinant GAPDH also provided good protection in a pig model. GAPDH antiserum exhibited significant blood bactericidal activity against E. rhusiopathiae. In conclusion, GAPDH and CbpB-N were found to be protective antigens of E. rhusiopathiae, and GAPDH is a promising vaccine candidate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vaccine.2018.10.071DOI Listing
December 2018
-->