Publications by authors named "Chao Jiang"

809 Publications

Neutrophil autophagy and NETosis in COVID-19: perspectives.

Autophagy 2022 Aug 11:1-10. Epub 2022 Aug 11.

Department of Pain Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.

The COVID-19 pandemic has caused substantial losses worldwide in people's lives, health, and property. Currently, COVID-19 is still prominent worldwide without any specific drug treatment. The SARS-CoV-2 pathogen is the cause of various systemic diseases, mainly acute pneumonia. Within the pathological process, neutrophils are recruited to infected sites, especially in the lungs, for the first stage of removing invading SARS-CoV-2 through a range of mechanisms. Macroautophagy/autophagy, a conserved autodegradation process in neutrophils, plays a crucial role in the neutrophil phagocytosis of pathogens. NETosis refers to neutrophil cell death, while auto-inflammatory factors and antigens release NETs. This review summarizes the latest research progress and provides an in-depth explanation of the underlying mechanisms of autophagy and NETosis in COVID-19. Furthermore, after exploring the relationship between autophagy and NETosis, we discuss potential targets and treatment options. This review keeps up with the latest research on COVID-19 from neutrophil autophagy and NETosis with a new perspective, which can guide the urgent development of antiviral drugs and provide guidance for the clinical treatment of COVID-19. AKT1: AKT serine/threonine kinase 1; AMPK: AMP-activated protein kinase; AP: autophagosome; ARDS: acute respiratory distress syndrome; ATG: autophagy related; BECN1: beclin 1; cfDNA: cell-free DNA; COVID-19: coronavirus disease 2019; CQ: chloroquine; DMVs: double-membrane vesicles; ELANE/NE: elastase, neutrophil expressed; F3: coagulation factor III, tissue factor; HCQ: hydroxychloroquine; MAP1LC3/LC3: microtubule associated protein 1 light chain of 3; MPO: myeloperoxidase; MTORC1: mechanistic target of rapamycin kinase complex 1; NETs: neutrophil traps; NSP: nonstructural protein; PI3K: class I phosphoinositide 3-kinase; PtdIns3K: class III phosphatidylinositol 3-kinase; PtdIns3P: phosphatidylinositol-3-phosphate; ROS: reactive oxygen species; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; SKP2: S-phase kinase associated protein 2; TCC: terminal complement complex; ULK1: unc-51 like.
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http://dx.doi.org/10.1080/15548627.2022.2099206DOI Listing
August 2022

Accurate identification of taxon-specific molecular markers in plants based on DNA signature sequence.

Mol Ecol Resour 2022 Aug 11. Epub 2022 Aug 11.

National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences (CACMS), Beijing 100700, China.

Accurate identification of plants remains a significant challenge for taxonomists and is the basis for plant diversity conservation. Although DNA barcoding methods are commonly used for plant identification, these are limited by the low amplification success and low discriminative power of selected genomic regions. In this study, we developed a k-mer-based approach, the DNA signature sequence (DSS), to accurately identify plant taxon-specific markers, especially at the species level. DSS is a constant-length nucleotide sequence capable of identifying a taxon and distinguishing it from other taxa. In this study, we performed the first large-scale study of DSS markers in plants. DSS candidates of 3,899 angiosperm plant species were calculated based on a chloroplast dataset with 4,356 assemblies. Using Sanger sequencing of PCR amplicons and high-throughput sequencing, DSSs were validated in four and 165 species, respectively. Based on this, the universality of the DSSs was over 79.38%. Several indicators influencing DSS marker identification and detection have also been evaluated, and common criteria for DSS application in plant identification have been proposed.
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http://dx.doi.org/10.1111/1755-0998.13697DOI Listing
August 2022

Association Between Atrial Fibrillation and Domain-Specific Cognitive Decline - Insights From the Systolic Blood Pressure Intervention Trial.

Circ J 2022 Aug 9. Epub 2022 Aug 9.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases.

Background: There is a dearth of evidence to characterize longitudinal changes in domain-specific cognitive function related to atrial fibrillation (AF).Methods and Results: This study enrolled 2,844 participants from the Systolic Blood Pressure Intervention Trial (SPRINT). Cognitive function was assessed at baseline and biennially during the follow-up period. Declines in global function and 4 major cognitive domains (i.e., memory, processing speed, language, and executive function) were fitted and compared between participants with and without AF using robust linear mixed-effect models. There were 252 participants with prevalent AF (mean [±SD] age 72.0±8.5 years; 30% women) and 2,592 participants without AF (mean age 67.9±8.4 years; 38% women). The annual decline in global function scores was greater among participants with than without AF (-0.016 vs. -0.012 points); however, the difference was not statistically significant (P=0.33). Processing speed declined faster in participants with prevalent AF, with a distinct difference of -0.013 points/year (95% CI -0.024~-0.001 points/year; P=0.02). For the memory, executive function, and language domains, there were no significant differences in the rate of cognitive decline between participants with and without AF.

Conclusions: In this post hoc analysis of the SPRINT trial, processing speed was the most prominent cognitive domain affected by AF, which may be beneficial for the early screening of cognitive dysfunction.
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http://dx.doi.org/10.1253/circj.CJ-22-0224DOI Listing
August 2022

Arbutin-modified microspheres prevent osteoarthritis progression by mobilizing local anti-inflammatory and antioxidant responses.

Mater Today Bio 2022 Dec 19;16:100370. Epub 2022 Jul 19.

Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

Osteoarthritis (OA) is a common degenerative joint disease worldwide and currently there is no effective strategy to stop its progression. It is known that oxidative stress and inflammation can promote the development of OA, and therapeutic strategies against these conditions may alleviate OA. Arbutin (ARB), a major ingredient of the Chinese medicinal herb cowberry leaf, exerts good antioxidant and anti-inflammatory activities yet has not been studied in OA. Here we developed ARB-loaded gelatine methacryloyl-Liposome ([email protected]) microspheres which showed long-term release of ARB and excellent cartilage-targeting effects. The ARB-loaded microspheres effectively reduced the inflammatory response in interleukin (IL)-1β-treated arthritic chondrocytes. Moreover, the synthesized [email protected] microspheres regulated cartilage extracellular matrix (ECM) homeostasis through anti-inflammation effect via inhibiting NF-κB signaling and anti-oxidative stress effect via activating Nrf2 pathway. Intra-articular use of [email protected] can effectively reduce inflammation and oxidative stress in the articular cartilage and thus, attenuating OA progression in a mouse model. The study proposed a novel ARB-laden functional microsphere, [email protected], and demonstrated that this compound may be used as an alternative therapeutics for treating OA.
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http://dx.doi.org/10.1016/j.mtbio.2022.100370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352975PMC
December 2022

Effect of Modified Vertical Rectus Belly Transposition vs Augmented Superior Rectus Transposition Plus Medial Rectus Recession for Chronic Sixth Nerve Palsy: A Randomized Clinical Trial.

JAMA Ophthalmol 2022 Aug 4. Epub 2022 Aug 4.

Department of Ophthalmology and Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China.

Importance: Both vertical rectus belly transposition (VRBT) and superior rectus transposition (SRT) can be performed simultaneously with ipsilateral medial rectus recession (MRc) and have been shown to be effective for chronic sixth nerve palsy. However, it is unclear whether VRBT is superior to SRT in correcting esotropia.

Objective: To compare the effectiveness of modified VRBT plus MRc (mVRBT-MRc) vs augmented SRT plus MRc (aSRT-MRc) in Chinese patients with chronic sixth nerve palsy.

Design, Setting, And Participants: This parallel-design, double-masked, single-center, randomized clinical trial was conducted from January 15, 2018, to May 24, 2021. The follow-up visits were scheduled at 1 month and 6 months. Eligible Chinese participants with unilateral chronic sixth nerve palsy were randomly assigned to receive either mVRBT-MRc (VRBT group) or aSRT-MRc (SRT group).

Interventions: mVRBT-MRc or aSRT-MRc.

Main Outcomes And Measures: Change of horizontal deviation in primary position from baseline to 6 months.

Results: Of the total 25 eligible participants, the mean (SD) age was 45.4 (12.6) years, with 10 male participants (40%) and 15 female participants (60%). Thirteen participants (52%) were randomly assigned to the VRBT group, and 12 (48%) were randomly assigned to the SRT group. At baseline, the mean (SD) horizontal deviation was 65.7 (10.8) prism diopters (Δ) in the VRBT group and 60.5Δ(14.1Δ) in the SRT group. Similar amounts of MRc were performed in both groups. At 6 months, the horizontal deviation changed from baseline by 66.3Δ in the VRBT group and by 51.5Δ in the SRT group. The adjusted group difference was 10.9Δ (95% CI, 5.3Δ-16.6Δ), favoring the VRBT group (P = .001). Four times as many participants corrected more than 60Δ with mVRBT-MRc compared with aSRT-MRc. The group difference of the improvement of abduction limitation was -0.2 (95% CI, -0.8 to 0.5; P = .64). Although there was a higher proportion of undercorrection in the SRT group (difference, 45%; 95% CI, 16%-75%; P = .01), no differences were identified for other suboptimal outcomes between groups.

Conclusions And Relevance: Compared with aSRT-MRc, mVRBT-MRc showed better effect in correcting esotropia with no differences detected for other suboptimal outcomes. mVRBT-MRc may be a promising alternative surgical procedure for chronic sixth nerve palsy, particularly for large esotropia of more than 60Δ, if these results are confirmed in larger, diverse cohorts with longer follow-up.

Trial Registration: ChiCTR Identifier: ChiCTR-INR-17013705.
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http://dx.doi.org/10.1001/jamaophthalmol.2022.2856DOI Listing
August 2022

Genome-wide DNA methylation signature predict clinical benefit of bevacizumab in non-small cell lung cancer.

BMC Cancer 2022 Jul 29;22(1):828. Epub 2022 Jul 29.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, 440 Jiyan Road, Jinan, 250117, Shandong, China.

Background: The efficacy of bevacizumab in non-small cell lung cancer (NSCLC) patients is unsatisfactory, and the selection of suitable patients is still challenging. Given the epigenetic modifications can contribute to an aberrant regulation of angiogenesis and microenvironment, we investigated DNA methylation profiles to determine clinical benefit of bevacizumab in NSCLC patients.

Methods: Genome-wide DNA methylation profiling was performed in NSCLC patients treated with chemotherapy in combination with bevacizumab. Patients were divided into better prognosis group (A group) and inferior prognosis group (B group) based on their survival. The difference of methylation patterns and respective functional enrichment analysis were performed between two groups. Prognostic DNA methylation signature for bevacizumab was established with the least absolute shrinkage and selection operator regression analyses. TISIDB database was further used to infer immunological relationship for prognostic related DNA methylation.

Results: Twenty patients were included in this study, and significantly distinct methylation patterns were observed between patients with different prognosis. Related genes of different methylation regions were significantly enriched in the biological process of cell projection assembly, neutrophil mediated immunity, and pathway of VEGFA-VEGFR2 signaling pathway, neutrophil degranulation. A 10-gene DNA methylation signature for prognosis prediction was established with the C-index of 0.76. And host genes of signature were found to be related to the abundance of ActCD4, Th1, ActCD8, NKT and neutrophil cells.

Conclusion: The 10-gene DNA methylation signature could serve as a novel biomarker to predict the clinical benefit of bevacizumab therapy and improve this anti-tumor approach for NSCLC patients.
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http://dx.doi.org/10.1186/s12885-022-09918-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338664PMC
July 2022

Analysis Model of Image Colour Data Elements Based on Deep Neural Network.

Comput Intell Neurosci 2022 18;2022:7631788. Epub 2022 Jul 18.

School of Arts, Anhui University of Finance and Economics, Bengbu 233030, Anhui, China.

At present, the classification method used in image colour element analysis in China is still based on subjective visual evaluation. Because the evaluation process will inevitably be disturbed by human factors, it will not only have low efficiency but also produce large errors. To solve the above problems, this paper proposes an image colour data element analysis model based on depth neural network. Firstly, intelligent analysis of image colour data elements based on tensorflow is constructed, and the isomerized tensorflow framework is designed with the idea of Docker cluster to improve the efficiency of image element analysis. Secondly, considering the time error and spatial error diffusion model in the process of image analysis, the quantization modified error diffusion model is replaced by the original model for more accurate colour management. Image colour management is an important link in the process of image reproduction; the rotating principal component analysis method is used to correct and analyze the image colour error. Finally, using the properties of transfer learning and convolution neural network, an image colour element analysis model based on depth neural network is established. Large-scale image data is collected, and the effectiveness and reliability of the algorithm are verified from different angles. The results show that the new image colour analysis method can not only reveal the true colour components of the target image; furthermore, the real colour component of the target image also has high spectral data reconstruction accuracy, and the analysis results have strong adaptability.
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http://dx.doi.org/10.1155/2022/7631788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313933PMC
July 2022

Densely Connected Neural Networks for Nonlinear Regression.

Entropy (Basel) 2022 Jun 25;24(7). Epub 2022 Jun 25.

Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China.

Densely connected convolutional networks (DenseNet) behave well in image processing. However, for regression tasks, convolutional DenseNet may lose essential information from independent input features. To tackle this issue, we propose a novel DenseNet regression model where convolution and pooling layers are replaced by fully connected layers and the original concatenation shortcuts are maintained to reuse the feature. To investigate the effects of depth and input dimensions of the proposed model, careful validations are performed by extensive numerical simulation. The results give an optimal depth (19) and recommend a limited input dimension (under 200). Furthermore, compared with the baseline models, including support vector regression, decision tree regression, and residual regression, our proposed model with the optimal depth performs best. Ultimately, DenseNet regression is applied to predict relative humidity, and the outcome shows a high correlation with observations, which indicates that our model could advance environmental data science.
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http://dx.doi.org/10.3390/e24070876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317522PMC
June 2022

The Long-Term Efficacy, Prognostic Factors, Safety, and Hospitalization Costs Following Denervation and Myotomy of the Affected Muscles and Deep Brain Stimulation in 94 Patients with Spasmodic Torticollis.

Brain Sci 2022 Jul 4;12(7). Epub 2022 Jul 4.

Cadre Medical Department, The First Medical Clinical Center, PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.

The surgical methods for treating spasmodic torticollis include the denervation and myotomy (DAM) of the affected muscles and deep brain stimulation (DBS). This study reports on the long-term efficacy, prognostic factors, safety, and hospitalization costs following these two procedures. We collected data from 94 patients with spasmodic torticollis, of whom 41 and 53 were treated with DAM and DBS, respectively, from June 2008 to December 2020 at the Chinese People's Liberation Army General Hospital. We used the Tsui scale and the global outcome score of the Toronto Western Spasmodic Torticollis Rating Scale (TWSTRS) to evaluate the preoperative and postoperative clinical conditions in all patients. We also determined the costs of hospitalization, prognostic factors, and serious adverse events following the two surgical procedures. The mean follow-up time was 68.83 months (range = 13-116). Both resection surgery and DBS showed good results in terms of Tsui (Z = -5.103, = 0.000; Z = -6.210, = 0.000) and TWSTRS scores (t = 8.762, = 0.000; Z = -6.308, = 0.000). Compared with the DAM group, the preoperative (47.71, range 24-67.25) and postoperative (18.57, range 0-53) TWSTRS scores in the DBS group were significantly higher (Z = -3.161, = 0.002). We found no correlation between prognostic factors and patient age, gender, or disease duration for either surgical procedure. However, prognostic factors were related to the length of the postoperative follow-up period in the DBS surgery group (Z = -2.068, = 0.039; Z = -3.287, = 0.001). The mean hospitalization cost in the DBS group was 6.85 times that found in the resection group (Z = -8.284, = 0.000). The total complication rate was 4.26%. We found both resection surgery and DBS showed good results in the patients with spasmodic torticollis. Compared with DAM, DBS had a greater improvement in TWSTRS score; however, it was more expensive. Prognostic factors were related to the length of the postoperative follow-up period in patients who underwent DBS surgery.
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http://dx.doi.org/10.3390/brainsci12070881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313216PMC
July 2022

Hearing loss and depressive symptoms in older Chinese: whether social isolation plays a role.

BMC Geriatr 2022 07 26;22(1):620. Epub 2022 Jul 26.

School of Public Health, Sun Yat-Sen University, No. 74 Zhongshan 2ndRoad, Guangzhou, Guangdong Province, China.

Background: Existing evidence links hearing loss to depressive symptoms, with the extent of association and underlying mechanisms remaining inconclusive. We conducted a cross-sectional study to examine the association of hearing loss with depressive symptoms and explored whether social isolation mediated the association.

Methods: Eight thousand nine hundred sixty-two participants from Guangzhou Biobank Cohort Study were included. Data on self-reported hearing status, the 15-item Geriatric Depression Scale (GDS-15), social isolation and potential confounders were collected by face-to-face interview.

Results: The mean (standard deviation) age of participants was 60.2 (7.8) years. The prevalence of poor and fair hearing was 6.8% and 60.8%, respectively. After adjusting for age, sex, household income, education, occupation, smoking, alcohol use, self-rated health, comorbidities, compared with participants who had normal hearing, those with poor hearing (β = 0.74, 95% confidence interval (CI) 0.54, 0.94) and fair hearing (β = 0.59, 95% CI 0.48, 0.69) had higher scores of GDS-15. After similar adjustment, those with poor hearing (odds ratio (OR) = 2.13, 95% CI 1.65, 2.74) or fair hearing (OR = 1.68, 95% CI 1.43, 1.99) also showed higher odds of depressive symptoms. The association of poor and fair hearing with depressive symptoms attenuated slightly but not substantially after additionally adjusting for social isolation. In the mediation analysis, the adjusted proportion of the association mediated through social isolation was 9% (95% CI: 6%, 22%).

Conclusion: Poor hearing was associated with a higher risk of depressive symptoms, which was only partly mediated by social isolation. Further investigation of the underlying mechanisms is warranted.
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http://dx.doi.org/10.1186/s12877-022-03311-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316428PMC
July 2022

Chinese medical dialogue information extraction via contrastive multi-utterance inference.

Brief Bioinform 2022 07;23(4)

Hunan Provincial Key Laboratory of Intelligent Computing and Language Information Processing, Hunan Normal University, Changsha 410081, China.

Medical Dialogue Information Extraction (MDIE) is a promising task for modern medical care systems, which greatly facilitates the development of many real-world applications such as electronic medical record generation, automatic disease diagnosis, etc. Recent methods have firstly achieved considerable performance in Chinese MDIE but still suffer from some inherent limitations, such as poor exploitation of the inter-dependencies in multiple utterances, weak discrimination of the hard samples. In this paper, we propose a contrastive multi-utterance inference (CMUI) method to address these issues. Specifically, we first use a type-aware encoder to provide an efficient encode mechanism toward different categories. Subsequently, we introduce a selective attention mechanism to explicitly capture the dependencies among utterances, which thus constructs a multi-utterance inference. Finally, a supervised contrastive learning approach is integrated into our framework to improve the recognition ability for the hard samples. Extensive experiments show that our model achieves state-of-the-art performance on a public benchmark Chinese-based dataset and delivers significant performance gain on MDIE as compared with baselines. Specifically, we outperform the state-of-the-art results in F1-score by 2.27%, 0.55% in Recall and 3.61% in Precision (The codes that support the findings of this study are openly available in CMUI at https://github.com/jc4357/CMUI.).
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http://dx.doi.org/10.1093/bib/bbac284DOI Listing
July 2022

Progress and Prospects of Research on Public Services in Sports in China: Analysis in the Context of Bibliometric Ecological Civilization and Social Networks.

J Environ Public Health 2022 7;2022:9309074. Epub 2022 Jul 7.

School of Physical Education, Chaohu University, Hefei 238024, China.

In order to better understand the current situation of Chinese public sports, this paper studies Chinese public sports. According to SATI3.2 statistics, 71 newsletters have published a total of 903 articles, of which 40 newsletters have one article, 19.7% of the newsletters have 2-9 books, and 17 magazines have more than 10 issues. The results show that by measuring data, we can better understand the current state of China's public services. This paper uses the method of bibliometric analysis to sort out and summarize the policies, characteristics, hotspots, and trends of China's public service field under the social network environment. The survey shows that the education sector has paid less for public entertainment in recent years. The research direction is system, equity, government procurement, interest, capital allocation, strategy, and supply chain. This paper highlights that the shortcomings of public sports research in our country: "public sports services" and "public sports services" are not integrated; there are many macrostudies on public service sports but few microstudies; there is no track and field research for special public groups. This paper puts forward research guidance for China's public sports services and, at the same time, strengthens the research on public services of different products of rural sports development, strengthens the research on the intelligence of sports public services, and strengthens the research on the role of public sports. Governments at all levels support and strengthen research on public sports service standards, strengthen research on public sports performance measurement measures, and strengthen public sports balance research.
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http://dx.doi.org/10.1155/2022/9309074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283001PMC
July 2022

Validation of T-MoCA in the Screening of Mild Cognitive Impairment in Chinese Patients With Atrial Fibrillation.

Front Cardiovasc Med 2022 22;9:896846. Epub 2022 Jun 22.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, Beijing, China.

Background: Atrial fibrillation (AF) is associated with a high risk of mild cognitive impairment (MCI) and dementia. However, feasible and simple instruments that facilitate the regular assessment of cognitive status in patients with AF remain underdeveloped.

Methods: Cognitive function was first evaluated using telephone Montreal cognitive assessment (T-MoCA), and then patients were invited for an in-person interview for cognitive assessment using both Clinical Dementia Rating (CDR) and mini-mental status evaluation (MMSE). Using CDR = 0.5 as a reference standard, the ability of T-MoCA and MMSE to discriminate cognitive dysfunction, stratified by education level, was tested by receiver-operating curve (ROC) analysis. The net reclassification index was calculated for comparison between the performance of T-MoCA and MMSE.

Results: One hundred and one patients completed both telephone and in-person interview. Thirty-five MCI patients were identified as MCI using the criteria of CDR = 0.5. The areas under the ROC curve of T-MoCA were 0.80 (0.71-0.89), 0.83 (0.71-0.95), and 0.85 (0.64-0.92) for all patients, patients with high educational level, and patients with low education level, respectively. The optimal threshold was achieved at 16/17 with a sensitivity of 85.7% and a specificity of 69.7% in overall patients, 15/16 with a sensitivity of 88.2% and a specificity of 64.5% in the low educational level patients, and 16/17 with a sensitivity of 77.8% and a specificity of 87.9% in the high educational level patients. Compared to the criterion MMSE ≤ 27 and MMSE norms for the elderly Chinese community, the stratified T-MoCA threshold improves correct classification by 23.7% ( = 0.033) and 30.3% ( = 0.020), respectively.

Conclusion: T-MoCA is a feasible and effective instrument for MCI screening in patients with AF.
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http://dx.doi.org/10.3389/fcvm.2022.896846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257241PMC
June 2022

Pexmetinib suppresses osteoclast formation and breast cancer induced osteolysis via P38/STAT3 signal pathway.

J Bone Oncol 2022 Aug 11;35:100439. Epub 2022 Jun 11.

Department of Orthopaedics, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Breast cancer metastases to the bone can lead to a series of bone-related events that seriously affect the quality of life. Pexmetinib, a novel p38 mitogen-activated protein kinase (p38) inhibitor that has been evaluated in phase I clinical trials for myelodysplastic syndrome, but the effects of Pexmetinib on breast cancer induced osteolysis haven't been explored. Here, we found that Pexmetinib inhibited receptor activator of nuclear factor-κB ligand-induced osteoclast formation and bone resorption . Pexmetinib suppressed p38-mediated signal transducer and activator of transcription 3 (STAT3), which direct regulated transcription of the nuclear factor of activated T cells 1 (NFATc1), leading to reduced osteoclast formation. Moreover, Pexmetinib exerted anti-tumor effects in breast cancer cells via suppressing p38-mediated STAT3 activation and matrix metalloproteinases (MMPs) expression. Furthermore, Pexmetinib suppressed breast cancer-associated osteolysis . These results suggest that Pexmetinib may be a promising drug for the treatment of breast cancer-induced osteolysis.
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http://dx.doi.org/10.1016/j.jbo.2022.100439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253705PMC
August 2022

A network pharmacology approach to explore and validate the potential targets of ginsenoside on osteoporosis.

Int J Immunopathol Pharmacol 2022 Jan-Dec;36:3946320221107239

Department of Otorhinolaryngology Head and Neck Surgery, 34708Shandong Provincial Hospital affiliated to Shandong First Medical University, Jinan, China.

Osteoporosis (OP) is determined as a chronic systemic bone disorder to increase the susceptibility to fracture. Ginsenosides have been found the anti-osteoporotic activity of in vivo and in vitro. However, its mechanism remains unknown. The potential mechanism of ginsenosides in anti-osteoporotic activity was identified by using network phamacology analysis. The active compounds of ginsenosides and their targets associated to OP were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, Drug Bank, Pharmmapper, and Cytoscape. The Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis target genes were performed in String, Phenopedia, DisGeNET database, and Metascape software. The protein to protein interaction were created by String database and Cytoscape software. The molecular docking was used to investigate the interactions between active coumpounds and potential targets by utilizing SwissDock tool, UCSF Chimera, and Pymol software. A total of eight important active ingredients and 17 potential targets related to OP treatment were subjected to analyze. GO analysis showed the anti-osteoporosis targets of ginsenoside mainly play a role in the response to steroid hormone. KEGG enrichment analysis indicated that ginsenoside treats OP by osteoblast differentiation signal pathway. Lastly, the molecular docking outcomes indicated that ginsenoside rh2 had a good binding ability with four target proteins IL1B, TNF, IFNG, and NFKBIA. IL1B, TNF, IFNG, and NFKBIA are the most important targets and osteoblast differentiation is the most valuable signaling pathways in ginsenoside for the treatment of OP, which might be beneficial to elucidate the mechanism concerned to the action of ginsenoside and might supply a better understanding of its anti-OP effects.
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http://dx.doi.org/10.1177/03946320221107239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272184PMC
July 2022

Corrigendum to "Bone marrow mononuclear cells exert long-term neuroprotection in a rat model of ischemic stroke by promoting arteriogenesis and angiogenesis" [Brain Behav. Immun. 34 (2013) 56-66].

Brain Behav Immun 2022 Jun 27;104:213-214. Epub 2022 Jun 27.

Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA. Electronic address:

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http://dx.doi.org/10.1016/j.bbi.2022.04.025DOI Listing
June 2022

Suppression of osteoclast multinucleation via a posttranscriptional regulation-based spatiotemporally selective delivery system.

Sci Adv 2022 07 29;8(26):eabn3333. Epub 2022 Jun 29.

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310016, China.

Redundancy of multinucleated mature osteoclasts, which results from the excessive fusion of mononucleated preosteoclasts (pOCs), leads to osteolytic diseases such as osteoporosis. Unfortunately, the currently available clinical drugs completely inhibit osteoclasts, thus interfering with normal physiological bone turnover. pOC-specific regulation may be more suitable for maintaining bone homeostasis. Here, circBBS9, a previously unidentified circular RNA, was found to exert regulatory effects via the circBBS9/miR-423-3p/Traf6 axis in pOCs. To overcome the long-standing challenge of spatiotemporal RNA delivery to cells, we constructed biomimetic nanoparticles to achieve the pOC-specific targeted delivery of circBBS9. pOC membranes (POCMs) were extracted to camouflage cationic polymer for RNA interference with circBBS9 ([email protected]/shRNA). POCM-NPs endowed the nanocarriers with improved stability, accurate pOC targeting, fusogenic uptake, and reactive oxygen species-responsive release. In summary, our findings may provide an alternative strategy for multinucleated cell-related diseases that involves restriction of mononucleated cell multinucleation through a spatiotemporally selective delivery system.
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http://dx.doi.org/10.1126/sciadv.abn3333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242458PMC
July 2022

Interactions between the Autonomic Nervous System and the Immune System after Stroke.

Compr Physiol 2022 06 29;12(3):3665-3704. Epub 2022 Jun 29.

Department of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.

Acute stroke is one of the leading causes of morbidity and mortality worldwide. Stroke-induced immune-inflammatory response occurs in the perilesion areas and the periphery. Although stroke-induced immunosuppression may alleviate brain injury, it hinders brain repair as the immune-inflammatory response plays a bidirectional role after acute stroke. Furthermore, suppression of the systemic immune-inflammatory response increases the risk of life-threatening systemic bacterial infections after acute stroke. Therefore, it is essential to explore the mechanisms that underlie the stroke-induced immune-inflammatory response. Autonomic nervous system (ANS) activation is critical for regulating the local and systemic immune-inflammatory responses and may influence the prognosis of acute stroke. We review the changes in the sympathetic and parasympathetic nervous systems and their influence on the immune-inflammatory response after stroke. Importantly, this article summarizes the mechanisms on how ANS regulates the immune-inflammatory response through neurotransmitters and their receptors in immunocytes and immune organs after stroke. To facilitate translational research, we also discuss the promising therapeutic approaches modulating the activation of the ANS or the immune-inflammatory response to promote neurologic recovery after stroke. © 2022 American Physiological Society. Compr Physiol 12:3665-3704, 2022.
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http://dx.doi.org/10.1002/cphy.c210047DOI Listing
June 2022

Integrating Multiple Omics Identifies Acting as Marker Fungus to Promote Agarwood Sesquiterpene Accumulation by Inducing Plant Host Phosphorylation.

Microbiol Spectr 2022 Jun 28:e0272221. Epub 2022 Jun 28.

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

The present study aimed to explore the factors that promote persistent agarwood accumulation. To this end, we first investigated the morphological changes and volatile compound distribution in five layers of "Guan Xiang" agarwood. The agarwood-normal transition layer (TL), an essential layer of persistent agarwood accumulation, showed clear metabolic differences by microscopy and GC-MS analysis. Microbiome analysis revealed that was the predominant biomarker fungus in the TL of "Guan Xiang" agarwood samples. Among the seven isolated fungi, exhibited a significantly heightened ability to induce the production in seedlings, especially for sesquiterpene. Tracing the proteome profile changes in -induced calli for 18 ds showed that the fungus-induced sesquiterpene biosynthesis increased mainly through the mevalonate (MVA) pathway. Specifically, the phosphorylation modification level, instead of the protein abundance of transcription factors (TFs), showed corresponding changes during sesquiterpene biosynthesis, thus indicating that induced phosphorylation is the key reason for enhanced sesquiterpene production. Agarwood is an expensive resinous portion derived from plants and has been widely used as medicine, incense, and perfume. The factors involved in steady agarwood accumulation remain elusive. Our current study suggests that as a TL marker fungus, could persistently promote agarwood sesquiterpene accumulation by inducing phosphorylation of the TFs-MVA network in . Moreover, our work provides strategies to improve agarwood industry management and sheds light on the potential molecular mechanisms of plant adaptation to native microbial conditions.
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http://dx.doi.org/10.1128/spectrum.02722-21DOI Listing
June 2022

[Cumulative effects of -function in the research of point patterns].

Ying Yong Sheng Tai Xue Bao 2022 May;33(5):1275-1282

Inner Mongolia Meteorological Institute, Hohhot 010051, China.

The spatial pattern of plant population is one of primary issues in ecological research. Point pattern analy-sis is considered as an important method to study the spatial pattern of plant population. Ripley's function has been commonly used for point pattern analysis. However, the cumulative effect of Ripley's function may lead to specific spatial pattern charcteristics. To explore how the cumulative effect of Ripley's function affects population pattern, the data of clumped distribution, random distribution and regular distribution of were simulated by R software. All data generated by R software were analyzed by Ripley's function and the non-cumulative pairwise correlation function (). The results showed that for clumped distribution (or regular distribution), the cumulative effect of Ripley's function was manifested in two aspects. On the one hand, the scale of clumped distribution (or regular distribution) was increased due to Ripley's function. On the other hand, Ripley's function could detect the difference of the distribution of cluster (or negative interaction range) in the sampling space, exhibiting different pattern characteristics. For random distribution, Ripley's function had no cumulative effect. In conclusion, the combination of Ripley's function and pairwise correlation function by collecting replicate samples could better reveal the essential characteristics of the pattern in the study of population pattern.
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http://dx.doi.org/10.13287/j.1001-9332.202205.005DOI Listing
May 2022

The changes of microplastics' behavior in adsorption and anaerobic digestion of waste activated sludge induced by hydrothermal pretreatment.

Water Res 2022 Aug 14;221:118744. Epub 2022 Jun 14.

Department of Environmental Science and Engineering, Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Fudan University, Shanghai 200438, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai 200438, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Waste activated sludge (WAS) contains high concentrations of microplastics (MPs), which could serve as vectors of various organic pollutants and heavy metals, causing synergistic transportation and pollution. The application of combined hydrothermal pretreatment (HTP) and anaerobic digestion (AD) has raised growing concerns since the low-temperature hydrothermal treatment could enhance the biogas production of WAS. However, the changes in physicochemical properties, adsorption performances, and effects on AD of MPs by HTP have not been studied. The study used three typical MPs in WAS, and it was found that the HTP (170°C & 30min) increased MPs' specific surface area and carbonyl index (CI) while decreasing the relative crystallinity. The adsorption capacity to Cd increased through the carbonylation for polyethylene microplastic (PE-MP) and polystyrene microplastic (PS-MP) while decreasing by the dechlorination for polyvinyl chloride microplastic (PVC-MP). Meanwhile, increased hydrophilicity reduced the adsorption capacities of all three typical MPs for ofloxacin. The above results indicated that the HTP could be worth blocking the adsorption of polar MPs for polar pollutants. For the pristine MPs, only PVC-MP at the highest concentration (0.5 g kg VS) significantly (p < 0.05) reduced methane production by 16.2 ± 3.3% of WAS without the HTP. However, the HTP resulted in significant (p < 0.05) inhibition of methane production of WAS at high concentrations of PE-MP and PVC-MP (e.g., 0.1 and 0.5 g kg VS), which was due to the acceleration of the released toxic plastic additives (dibutyl phthalate, dimethyl phthalate, and bisphenol-A). Microbial analysis showed the abundances of vital anaerobes, such as acid-producing bacteria (Acetoanerrobium and Mesotoga), proteolytic bacteria (Proteiniborus), and methanogens (Methanosaeta) clearly decreased with the PE-MP and PVC-MP after the HTP, which might result in the decreased methane production. The study provided deep-insight of MPs' behaviors during the combined HTP-AD process.
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http://dx.doi.org/10.1016/j.watres.2022.118744DOI Listing
August 2022

Heterogeneous lattice strain strengthening in severely distorted crystalline solids.

Proc Natl Acad Sci U S A 2022 Jun 13;119(25):e2200607119. Epub 2022 Jun 13.

Department of Materials Science and Engineering; Mechanical Behavior Division of Shenyang National Laboratory for Materials Science, College of Engineering, City University of Hong Kong, Kowloon Tong, Kowloon, Hong Kong SAR 999077, China.

Multi-principal element alloys (MPEAs) exhibit outstanding mechanical properties because the core effect of severe atomic lattice distortion is distinctly different from that of traditional alloys. However, at the mesoscopic scale the underlying physics for the abundant dislocation activities responsible for strength-ductility synergy has not been uncovered. While the Eshelby mean-field approaches become insufficient to tackle yielding and plasticity in severely distorted crystalline solids, here we develop a three-dimensional discrete dislocation dynamics simulation approach by taking into account the experimentally measured lattice strain field from a model FeCoCrNiMn MPEA to explore the heterogeneous strain-induced strengthening mechanisms. Our results reveal that the heterogeneous lattice strain causes unusual dislocation behaviors (i.e., multiple kinks/jogs and bidirectional cross slips), resulting in the strengthening mechanisms that underpin the strength-ductility synergy. The outcome of our research sheds important insights into the design of strong yet ductile distorted crystalline solids, such as high-entropy alloys and high-entropy ceramics.
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http://dx.doi.org/10.1073/pnas.2200607119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231497PMC
June 2022

Lambl's excrescence and the safety of radiofrequency ablation for atrial fibrillation.

Pacing Clin Electrophysiol 2022 07 13;45(7):821-825. Epub 2022 Jun 13.

Department of Cardiology, National Clinical Research Center for Cardiovascular Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: Lambl's excrescences (LEs) are excrescences with an extremely low incidence, mainly ultrasound diagnosed. Increasingly, LEs are detected by transesophageal echocardiography before catheter ablation, which raises safety concerns on whether LEs were associated with an embolism event during or after ablation, but clinical data are still lacking.

Methods And Results: We consecutively recruited 8081 patients with atrial fibrillation who underwent radiofrequency catheter ablation in Beijing Anzhen Hospital from Jan 1, 2017 to Dec 31, 2019. Total 21 patients (0.3%) were diagnosed as LEs with an average age of 70.8 ± 8.9 years, and 38.1% were male. Persistent atrial fibrillation (PeAF) and paroxysmal atrial fibrillation (PAF) accounted for 57.1% (12 cases) and 42.9% (nine cases), respectively. LEs were mostly frequently observed on the aortic valve (18 cases, 75%) and mitral valve (six cases, 25%). Precisely, the noncoronary cusp is ranked first in terms of the LEs presence (seven cases, 29.2%), followed by the right coronary cusp (six cases, 25.0%), the left coronary cusp (five cases, 20.8%), the anterior mitral valve (four cases, 16.7%), and the posterior mitral valve (two cases, 8.3%). During the ablation for LEs patients, the average procedure time was 96.0 ± 22.4 min; the average fluoroscopy time was 4.2 ± 0.8 min; the average total ablation time was 20.6 ± 5.6 min; and the mean hospital stay was 3.3 ± 0.6 days. No patients suffered from serious complications during the procedure. Furthermore, no cardiovascular event was observed during a follow-up of 19.1 ± 11.8 months.

Conclusions: There was no clear association between LEs with intraoperative embolism events or cardiovascular events during the follow-up period.
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http://dx.doi.org/10.1111/pace.14518DOI Listing
July 2022

A Chinese multi-modal neuroimaging data release for increasing diversity of human brain mapping.

Sci Data 2022 06 9;9(1):286. Epub 2022 Jun 9.

State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, 100875, China.

The big-data use is becoming a standard practice in the neuroimaging field through data-sharing initiatives. It is important for the community to realize that such open science effort must protect personal, especially facial information when raw neuroimaging data are shared. An ideal tool for the face anonymization should not disturb subsequent brain tissue extraction and further morphological measurements. Using the high-resolution head images from magnetic resonance imaging (MRI) of 215 healthy Chinese, we discovered and validated a template effect on the face anonymization. Improved facial anonymization was achieved when the Chinese head templates but not the Western templates were applied to obscure the faces of Chinese brain images. This finding has critical implications for international brain imaging data-sharing. To facilitate the further investigation of potential culture-related impacts on and increase diversity of data-sharing for the human brain mapping, we released the 215 Chinese multi-modal MRI data into a database for imaging Chinese young brains, namely'I See your Brains (ISYB)', to the public via the Science Data Bank ( https://doi.org/10.11922/sciencedb.00740 ).
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http://dx.doi.org/10.1038/s41597-022-01413-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184635PMC
June 2022

Precision environmental health monitoring by longitudinal exposome and multi-omics profiling.

Genome Res 2022 Jun 6;32(6):1199-1214. Epub 2022 Jun 6.

Department of Genetics, Stanford University School of Medicine, Stanford, California 94304, USA.

Conventional environmental health studies have primarily focused on limited environmental stressors at the population level, which lacks the power to dissect the complexity and heterogeneity of individualized environmental exposures. Here, as a pilot case study, we integrated deep-profiled longitudinal personal exposome and internal multi-omics to systematically investigate how the exposome shapes a single individual's phenome. We annotated thousands of chemical and biological components in the personal exposome cloud and found they were significantly correlated with thousands of internal biomolecules, which was further cross-validated using corresponding clinical data. Our results showed that agrochemicals and fungi predominated in the highly diverse and dynamic personal exposome, and the biomolecules and pathways related to the individual's immune system, kidney, and liver were highly associated with the personal external exposome. Overall, this data-driven longitudinal monitoring study shows the potential dynamic interactions between the personal exposome and internal multi-omics, as well as the impact of the exposome on precision health by producing abundant testable hypotheses.
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http://dx.doi.org/10.1101/gr.276521.121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248886PMC
June 2022

Respiratory exposure to graphene oxide induces pulmonary fibrosis and organ damages in rats involving caspase-1/p38MAPK/TGF-β1 signaling pathways.

Chemosphere 2022 Sep 3;303(Pt 3):135181. Epub 2022 Jun 3.

International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), Heilongjiang Institute of Labor Hygiene and Occupational Diseases/The Second Hospital of Heilongjiang Province, Harbin, 150028, PR China.

Numerous studies have shown that graphene oxide (GO) respiratory exposure led to severe lung injury, but whether pulmonary fibrosis caused by GO respiratory exposure is related to the activation of the caspase-1/p38MAPK/TGF-β1 remains unclear. In this study, rats were administrated GO by intratracheal instillation and fed for three months, and the molecular mechanisms of GO on the pulmonary fibrosis and other organ damage caused by GO respiratory exposure were examined. The results showed that the expression of caspase-1/p38MAPK/TGF-β1 pathway-related factors were significantly elevated with the increase of exposure concentrations of GO. Those data proved that the caspase-1/p38MAPK/TGF-β1 signaling pathway was involved in the pulmonary fibrosis caused by GO respiratory exposure. The trends of related factors also proved that the caspase-1/p38MAPK/TGF-β1 pathway was likely to play a dominant role in the sub-acute and sub-chronic stages. The other organ damage examination found that the liver and spleen were damaged initially by the GO respiratory exposure. Meanwhile for the testicle, although the acute injury was severe, signs of recovery were found during the three-month trial period.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135181DOI Listing
September 2022

Deep Denoising of Raw Biomedical Knowledge Graph From COVID-19 Literature, LitCovid, and Pubtator: Framework Development and Validation.

J Med Internet Res 2022 07 6;24(7):e38584. Epub 2022 Jul 6.

Department of Artificial Intelligence and Informatics Research, Mayo Clinic, Rochester, MN, United States.

Background: Multiple types of biomedical associations of knowledge graphs, including COVID-19-related ones, are constructed based on co-occurring biomedical entities retrieved from recent literature. However, the applications derived from these raw graphs (eg, association predictions among genes, drugs, and diseases) have a high probability of false-positive predictions as co-occurrences in the literature do not always mean there is a true biomedical association between two entities.

Objective: Data quality plays an important role in training deep neural network models; however, most of the current work in this area has been focused on improving a model's performance with the assumption that the preprocessed data are clean. Here, we studied how to remove noise from raw knowledge graphs with limited labeled information.

Methods: The proposed framework used generative-based deep neural networks to generate a graph that can distinguish the unknown associations in the raw training graph. Two generative adversarial network models, NetGAN and Cross-Entropy Low-rank Logits (CELL), were adopted for the edge classification (ie, link prediction), leveraging unlabeled link information based on a real knowledge graph built from LitCovid and Pubtator.

Results: The performance of link prediction, especially in the extreme case of training data versus test data at a ratio of 1:9, demonstrated that the proposed method still achieved favorable results (area under the receiver operating characteristic curve >0.8 for the synthetic data set and 0.7 for the real data set), despite the limited amount of testing data available.

Conclusions: Our preliminary findings showed the proposed framework achieved promising results for removing noise during data preprocessing of the biomedical knowledge graph, potentially improving the performance of downstream applications by providing cleaner data.
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http://dx.doi.org/10.2196/38584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301549PMC
July 2022

Single layer aligned semiconducting single-walled carbon nanotube array with high linear density.

Nanotechnology 2022 Jun 20;33(37). Epub 2022 Jun 20.

CAS Key Laboratory of Standardization and Measurement for Nanotechnology & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China.

Highly ordered semiconducting single-walled carbon nanotubes(sc-SWCNTs) array with high purity, high linear density and controllable manner is strongly desired for carbon-based integrated circuits, yet it remains a big challenge. Herein, close-packed single layered and controllably aligned sc-SWCNTs arrays were obtained through dielectrophoresis using a high purity sc-SWCNT dispersion. Under optimized condition of length and average number of interconnecting junctions across the channel full of aligned sc-SWCNTs, field effect transistors (FETs) with high performance were achieved with both a high on/off current ratio and large carrier mobility. Based on the optimized channel length, by systematically optimizing the dielectrophoresis parameters of the frequency and duration of applied AC voltage (), the highly ordered sc-SWCNTs arrays with an ultra-high linear density of 54 ± 2 tubesmshowed relatively high device performance of FET. The fabrication process optimized in this report can be further extended and applied in large-area, low-cost carbon-based integrated circuits.
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http://dx.doi.org/10.1088/1361-6528/ac7574DOI Listing
June 2022

BETA: a comprehensive benchmark for computational drug-target prediction.

Brief Bioinform 2022 07;23(4)

Department of Artificial Intelligence and Informatics Research, Mayo Clinic, Rochester, MN.

Internal validation is the most popular evaluation strategy used for drug-target predictive models. The simple random shuffling in the cross-validation, however, is not always ideal to handle large, diverse and copious datasets as it could potentially introduce bias. Hence, these predictive models cannot be comprehensively evaluated to provide insight into their general performance on a variety of use-cases (e.g. permutations of different levels of connectiveness and categories in drug and target space, as well as validations based on different data sources). In this work, we introduce a benchmark, BETA, that aims to address this gap by (i) providing an extensive multipartite network consisting of 0.97 million biomedical concepts and 8.5 million associations, in addition to 62 million drug-drug and protein-protein similarities and (ii) presenting evaluation strategies that reflect seven cases (i.e. general, screening with different connectivity, target and drug screening based on categories, searching for specific drugs and targets and drug repurposing for specific diseases), a total of seven Tests (consisting of 344 Tasks in total) across multiple sampling and validation strategies. Six state-of-the-art methods covering two broad input data types (chemical structure- and gene sequence-based and network-based) were tested across all the developed Tasks. The best-worst performing cases have been analyzed to demonstrate the ability of the proposed benchmark to identify limitations of the tested methods for running over the benchmark tasks. The results highlight BETA as a benchmark in the selection of computational strategies for drug repurposing and target discovery.
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http://dx.doi.org/10.1093/bib/bbac199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294420PMC
July 2022

Effect of low-frequency noise on the survival rate and immunity of infected Vibrio parahaemolyticus sea slug (Onchidium reevesii).

Fish Shellfish Immunol 2022 Jul 26;126:227-236. Epub 2022 May 26.

National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals, Shanghai Ocean University, Shanghai, 201306, China. Electronic address:

Anthropogenic noise in the marine environment has become a global environmental pollutant that affects the behavior, physiology and immunity of marine animals. However, the resistance of marine animals to pathogens while under the influence of noise is a topic that has received little attention. To assess the immune defense response of sea slugs against pathogens when exposed to low frequency noise, we performed 120 h exposure experiments on sea slugs after a Vibrio parahaemolyticus application in low frequency noise at 500 Hz and 1000 Hz. We found that after the infection with V. parahaemolyticus, the survival rate of the sea slugs decreased, the apoptosis rate and reactive oxygen species (ROS) production of hemocytes increased significantly (P < 0.05), the proliferation of hemocytes accelerated, the activities of enzymes such as superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (AKP), alanine transaminase (ALT) and lysozyme (LZM) in the hepatopancreas increased significantly, and the expression of TNF signaling pathway-related genes (TNF-α, FADD, Caspase 8, Caspase 3) and Hsp70 genes were generally upregulated. In addition, exposure of sea slug after infected with V. parahaemolyticus to low frequency noise resulted in a significant increase in both antioxidant and immune parameters, which were positively correlated with frequency. The results showed that noise frequency and exposure time had an interactive effect on the above indicators. In summary, low-frequency noise exposure increases the risk of pathogenic infections in sea slugs and exacerbates the negative effects on the antioxidant capacity and immune metabolism of the organism.
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http://dx.doi.org/10.1016/j.fsi.2022.05.045DOI Listing
July 2022
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