Publications by authors named "Chao Jia"

108 Publications

Long non-coding RNA -derived miR-22-3p promotes skeletal muscle differentiation and regeneration by inhibiting HDAC4.

Mol Ther Nucleic Acids 2021 Jun 26;24:200-211. Epub 2021 Feb 26.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Emerging studies have indicated that long non-coding RNAs (lncRNAs) play important roles in skeletal muscle growth and development. Nevertheless, it remains challenging to understand the function and regulatory mechanisms of these lncRNAs in muscle biology and associated diseases. Here, we identify a novel lncRNA, , that is significantly upregulated during myoblast differentiation and is highly expressed in skeletal muscle. We validated that promotes myoblast differentiation . Mechanistically, gives rise to mature microRNA (miR)-22-3p, which inhibits its target gene, histone deacetylase 4 (), thereby increasing the downstream myocyte enhancer factor 2C () and ultimately promoting myoblast differentiation. Furthermore, , we documented that knockdown delays repair and regeneration following skeletal muscle injury and further causes a significant decrease in weight following repair of an injured muscle. Additionally, gives rise to miR-22-3p to restrict expression, thereby promoting the differentiation and regeneration of skeletal muscle. Given the conservation of between mice and humans, might constitute a promising new therapeutic target for skeletal muscle injury, skeletal muscle atrophy, as well as other skeletal muscle diseases.
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http://dx.doi.org/10.1016/j.omtn.2021.02.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957084PMC
June 2021

Novel Gross Deletion Mutations in Gene Associated With Congenital Insensitivity to Pain With Anhidrosis.

Front Pediatr 2021 4;9:638190. Epub 2021 Mar 4.

Department of Neonatology, Hebei Provincial Children's Hospital, Shijiazhuang, China.

Congenital insensitivity to pain with anhidrosis (CIPA) is a rare inherited autosomal recessive disorder characterized by insensitivity to noxious stimuli, anhidrosis, recurrent fever, and intellectual disability. CIPA is mainly caused by mutations in the neurotrophic tyrosine kinase receptor type 1 gene (). This study aims to identify pathogenic mutations underlying CIPA in two unrelated Chinese families. DNA was extracted from blood samples of patients and their available family members and subjected to whole exome sequencing (WES). Real-time PCR (qPCR), Gap-PCR, and Sanger sequencing were applied to verify the identified variants. We found novel compound gross deletion mutations [exon1-6 del (g.1-1258_10169del); exon5-7 del (g.6995_11999del)] of (MIM 191315) gene in family 1 and the compound heterozygous mutations [c.851-33T>A; exon5-7 del (g.6995_11999del)] in family 2. Interestingly, we discovered the intragenic novel gross deletion [exon5-7 del (g.6995_11999del)] mediated by recombination between Alu elements. The present study highlights two rare gross deletion mutations in the gene associated with CIPA in two unrelated Chinese families. The deletion of exon1-6 (g.1-1258_10169del) is thought to be the largest deletion reported to date. Our findings expand the mutation spectrum of mutations in the Chinese and could be useful for prenatal interventions and more precise pharmacological treatments to patients. WES conducted in our study is a convenient and useful tool for clinical diagnosis of CIPA and other associated disorders.
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http://dx.doi.org/10.3389/fped.2021.638190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969531PMC
March 2021

Meta-analysis of procalcitonin as a predictor for acute kidney injury.

Medicine (Baltimore) 2021 Mar;100(10):e24999

Department of Nephrology.

Background: Procalcitonin (PCT) was used for predicting the development of acute kidney injury (AKI) in several studies recently. We aimed to investigate the accuracy of PCT for predicting AKI in this study.

Methods: Studies that assessed the predictive performance of PCT for the development of AKI in adult patients were searched from Medline, Embase, and the Cochrane Library from inception to June 2020. We calculated the pooled sensitivities and specificities and the area under the summary receiver-operating characteristic (SROC) curves. I2 was used to test the heterogeneity and the potential heterogeneity was investigated by meta-regression.

Results: In total, 9 of 119 studies with 4852 patients were included, 1272 were diagnosed with AKI. In the overall analysis, the area under the SROC curve was 0.82 (95% CI, 0.79-0.85) and the pooled sensitivity and specificity were 0.76 (95% confidence interval [CI], 0.64-0.85) and 0.75 (95% CI, 0.61-0.86), respectively. In the subgroup analysis among septic patients, the pooled sensitivity and specificity were 0.59 (95% CI, 0.29-0.84) and 0.53 (95% CI, 0.31-0.74), and the area under the SROC was 0.57 (95% CI, 0.53-0.62).

Conclusion: PCT may be a potential predictor for the development of AKI.
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http://dx.doi.org/10.1097/MD.0000000000024999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969283PMC
March 2021

Ultrasound-Induced Microbubble Cavitation Combined with miR-34a-Loaded Nanoparticles for the Treatment of Castration-Resistant Prostate Cancer.

J Biomed Nanotechnol 2021 Jan;17(1):78-89

Currently chemotherapy drugs are usually used as first-line treatments for castration-resistant prostate cancer (CRPC), but they are ineffective and accompanied by serious side effects. MicroRNA-34a (miR-34a) simultaneously targets multiple genes related to the cell apoptosis in CRPC cells without obvious side effects. It has shown great potential in the treatment of CRPC. Previous studies focused on miR-34a increasing the sensitivity of chemotherapy drugs to chemoresistant prostate cancer cells. There are few researches on miR-34a alone in the treatment of CRPC. But the macromolecular miR-34a is difficult to enter the cell and is easily degraded by nuclease. Therefore, we constructed methoxy polyethylene glycol-polylacticco-glycolic acid-polylysine (mPEG-PLGA-PLL) nanoparticles to encapsulate miR-34a (miR-34a/NP). The results showed that miR-34a/NP protects miR-34a from degradation by nucleases and can be phagocytized by PC-3 CRPC cells. Ultrasound induces microbubble cavitation (UIMC) improves cell membrane permeability and capillary gaps, and further promotes miR-34a/NP to enter cells PC-3 and prostate cancer xenografts. The miR-34a/NP that enters the cell and tumor tissue releases miR-34a, which suppressed CRPC cells PC-3 proliferation, promoted its apoptosis, and inhibited the growth of CRPC xenografts. Our research verified that miR-34a/NP, especially combined with UIMC, has a significant anti-tumor effect on CRPC.
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http://dx.doi.org/10.1166/jbn.2021.3020DOI Listing
January 2021

Differences in the clinical characteristics and outcomes of COVID-19 patients in the epicenter and peripheral areas of the pandemic from China: a retrospective, large-sample, comparative analysis.

BMC Infect Dis 2021 Feb 24;21(1):206. Epub 2021 Feb 24.

Department of Respiratory and Critical Care Medicine, Clinical Research Center for Respiratory Disease, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Background: There is limited information on the difference in epidemiology, clinical characteristics and outcomes of the initial outbreak of the coronavirus disease (COVID-19) in Wuhan (the epicenter) and Sichuan (the peripheral area) in the early phase of the COVID-19 pandemic. This study was conducted to investigate the differences in the epidemiological and clinical characteristics of patients with COVID-19 between the epicenter and peripheral areas of pandemic and thereby generate information that would be potentially helpful in formulating clinical practice recommendations to tackle the COVID-19 pandemic.

Methods: The Sichuan & Wuhan Collaboration Research Group for COVID-19 established two retrospective cohorts that separately reflect the epicenter and peripheral area during the early pandemic. The epidemiology, clinical characteristics and outcomes of patients in the two groups were compared. Multivariate regression analyses were used to estimate the adjusted odds ratios (aOR) with regard to the outcomes.

Results: The Wuhan (epicenter) cohort included 710 randomly selected patients, and the peripheral (Sichuan) cohort included 474 consecutive patients. A higher proportion of patients from the periphery had upper airway symptoms, whereas a lower proportion of patients in the epicenter had lower airway symptoms and comorbidities. Patients in the epicenter had a higher risk of death (aOR=7.64), intensive care unit (ICU) admission (aOR=1.66), delayed time from illness onset to hospital and ICU admission (aOR=6.29 and aOR=8.03, respectively), and prolonged duration of viral shedding (aOR=1.64).

Conclusions: The worse outcomes in the epicenter could be explained by the prolonged time from illness onset to hospital and ICU admission. This could potentially have been associated with elevated systemic inflammation secondary to organ dysfunction and prolonged duration of virus shedding independent of age and comorbidities. Thus, early supportive care could achieve better clinical outcomes.
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http://dx.doi.org/10.1186/s12879-020-05728-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903397PMC
February 2021

The role of contrast-enhanced ultrasound in the diagnosis of malignant non-mass breast lesions and exploration of diagnostic criteria.

Br J Radiol 2021 Apr 9;94(1120):20200880. Epub 2021 Feb 9.

Department of Ultrasound, Shanghai General Hospital of Nanjing Medical University, Shanghai, China.

Objectives: To assess the value of contrast-enhanced ultrasound (CEUS) for diagnosing malignant non-mass breast lesions (NMLs) and to explore the CEUS diagnostic criteria.

Methods: A total of 116 patients with 119 NMLs detected by conventional US were enrolled. Histopathological results were used as the reference standard. The enhancement characteristics of NMLs in CEUS were compared between malignant and benign NMLs. The CEUS diagnostic criteria for malignant NMLs were established using independent diagnostic indicators identified by binary logistic regression analysis. The diagnostic performance of Breast Imaging Reporting and Data System-US (BI-RADS-US), CEUS, and BI-RADS-US combined with CEUS was evaluated and compared.

Results: Histopathological results showed 63 and 56 benign and malignant NMLs. Enhancement degree (OR = 5.75, = 0.003), enhancement area (OR = 4.25, = 0.005), and radial or penetrating vessels (OR = 7.54, = 0.003) were independent diagnostic indicators included to establish the CEUS diagnostic criteria. The sensitivity and specificity of BI-RADS-US, CEUS, and BI-RADS-US combined with CEUS were 100 and 30.2%, 80.4 and 74.6%, and 94.6 and 77.8%, respectively; the corresponding areas under the receiver operating characteristic curve (AUC) were 0.819, 0.775, and 0.885, respectively.

Conclusions: CEUS has a high specificity in malignant NML diagnosis based on the diagnostic criteria including enhancement degree, enhancement area, and radial or penetrating vessels, but with lower sensitivity than BI-RADS-US. The combination of CEUS and BI-RADS-US is an effective diagnostic tool with both high sensitivity and specificity for the diagnosis of malignant NMLs.

Advances In Knowledge: In this study, we assessed the diagnostic value of CEUS for malignant NMLs and constructed a feasible diagnostic criterion. We further revealed that the combination of CEUS and BI-RADS-US has a high diagnostic value for malignant NMLs.
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http://dx.doi.org/10.1259/bjr.20200880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010562PMC
April 2021

Preparation of responsive "dual-lock" nanoparticles and their application in collaborative therapy based on CuS coordination.

J Mater Chem B 2021 01 5;9(4):1049-1058. Epub 2021 Jan 5.

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China.

It is difficult for drug delivery systems to release drugs as expected, often leading to undesired side effects. To solve this problem, a CuS@MSN/DOX@MnO@membrane (CMDMm) was reasonably designed. It was introduced to release the drug by a double response, similar to using two keys to open two locks at the same time for one door. CuS@MSN was used as a photothermal therapy (PTT) material and carrier, and then the surface of CuS@MSN/DOX was sealed by MnO to prevent drug release in advance. MnO could be reduced and degraded in a tumor microenvironment. It was applied in MR imaging due to the T magnetism of Mn following the reduction of MnO. Finally, the 4T1 cell membrane was extracted and coated onto the surface of CuS@MSN/DOX@MnO, which served as a target for 4T1 tumor cells. A noteworthy phenomenon was that the fluorescence of DOX was quenched by the coordination between DOX and CuS, and this greatly improved the interaction between DOX and CuS@MSN. However, the coordination was weakened when DOX was protonated in a tumor microenvironment (∼pH 5.0), leading to the release of DOX and fluorescence recovery. The drug release experiments showed that the release efficiency was higher at pH 5.0 with 10 mmol L GSH. Through in vitro laser confocal imaging, it was successfully observed that DOX was reliably released in specific tumor cells according to the fluorescence recovery, and that there was no leakage in other cells. In short, effective double response drug release was successfully confirmed.
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http://dx.doi.org/10.1039/d0tb02490bDOI Listing
January 2021

Identification of a novel GRIN2D variant in a neonate with intractable epileptic encephalopathy-a case report.

BMC Pediatr 2021 Jan 4;21(1). Epub 2021 Jan 4.

Neonatology Department, Children's Hospital of Hebei Province, 133 Jianhua South Street, 050031, Shijiazhuang, Hebei Province, China.

Background: N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that mediate excitatory synaptic transmission in the central nervous system. The functional NMDA receptors are heterotetramers consisting mainly of two GluN1 and two GluN2 subunits. GluN2 is encoded by the GRIN2D gene. A few case series have shown that GRIN2D variants are linked to developmental and epileptic encephalopathy. In this article, we report a novel GRIN2D variant, namely c.2021C > A (p.T674K) in a neonate with intractable epileptic encephalopathy.

Case Presentation: A 12-day-old boy who had stiffness of the lower and upper extremities since birth was transferred from a local hospital to our department. On admission, the patient presented with head tilting backwards, staring, apnea and hypertonia of limbs. Video electroencephalogram showed continuous, generalized or multi-focal spike-wave and spike-and-slow wave discharges and hypsarrhythmia. A treatment regimen composed of phenobarbital, midazolam, levetiracetam and clonazepam was administered, which however led to only partial control of the seizure. Whole-exome sequencing identified c.2021C > A (p.T674K) in GRIN2D in the patient while such a mutation was not detected in the parents. The patient was hospitalized for 1 month and died of sudden cardio-respiratory arrest 2 weeks after discharge.

Conclusions: A novel variant of GRIN2D was identified in a neonate with epileptic encephalopathy. Epilepsy associated with this GRIN2D mutation is refractory to conventional anti-epileptic medications.
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http://dx.doi.org/10.1186/s12887-020-02462-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780707PMC
January 2021

Urgently reveal longly hidden toxicant in a familiar fabrication process of biomass-derived environment carbon material.

J Environ Sci (China) 2021 Feb 7;100:250-256. Epub 2020 Aug 7.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai 200438, China. Electronic address:

Biomass-derived N-doped carbon (BNC) is an important environmental material and widely used in the fields of water purification and soil remediation. However, the toxicant in the commonly used synthesis process of BNC materials have been largely ignored. Herein, we firstly report the presence of a highly toxic by-product (KCN) in the activation process of BNC materials consequential of the carbothermal reduction reaction. Because this carbothermal reduction reaction also regulates the N-doping and pore development of BNC materials, the KCN content directly relates with the properties of BNC material properties. Accordingly, a high KCN content (∽ 611 mg) can occur in the production process of per g BNC material with high specific surface area (∽ 3600 m/g). Because the application performance of BNC material is determined by the surface area and available N doping, therefore, production of a BNC material with high performance entails high risk. Undoubtedly, this study proves a completely new risk recognition on a familiar synthesis process of biomass-based material. And, strict protective device should be taken in fabrication process of biomass-derived carbon material.
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http://dx.doi.org/10.1016/j.jes.2020.08.001DOI Listing
February 2021

Predictors of Mortality During Initial Liver Transplant Hospitalization and Investigation of Causes of Death.

Ann Transplant 2020 Dec 4;25:e926020. Epub 2020 Dec 4.

Department of Transplant Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China (mainland).

BACKGROUND Liver transplant (LT) remains a life-saving procedure with a high mortality rate. The present study investigated the causes of death and sought to identify predictive factors of mortality during the initial LT hospitalization. MATERIAL AND METHODS We retrieved data on first-time adult recipients who underwent LT between November 2017 and October 2019 receiving grafts from donation after citizen's death. The risk factors for mortality during the initial LT hospitalization were confirmed by univariate analysis. We also analyzed the causes of death. RESULTS We enrolled 103 recipients, including 86 males and 17 females, with a mean age of 47.7 years. Thirty-eight (36.9%) recipients were labeled as non-cholestatic cirrhosis-related indications. Approximately 8% of all recipients had diabetes prior to LT. Induction therapy was used in 11 (10.7%) recipients, along with maintenance therapy. The median model for end-stage liver disease score at LT was 32.4 (21.4-38.4). The in-hospital mortality rate of LT recipients was 6.8% (7/103), and infections were responsible for most of the deaths (6/7). The 1 remaining death resulted from primary graft failure. Univariate analysis showed recipients with postoperative pneumonia (p2 mg/dL, and alanine transaminase on day 1 after LT >1800 µmol/L (all P<0.001) were much more likely to die. CONCLUSIONS In-hospital mortality of LT recipients was high, due in large part to infections. Acute hepatic necrosis, prolonged post-transplant ICU stays, certain types of postoperative infections, and postoperative liver and kidney dysfunction were potential risk factors for in-hospital mortality of LT recipients.
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http://dx.doi.org/10.12659/AOT.926020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722774PMC
December 2020

Transformation between 2D covalent organic frameworks with distinct pore hierarchy via exchange of building blocks with different symmetries.

Chem Commun (Camb) 2020 Dec 24;56(98):15418-15421. Epub 2020 Nov 24.

Advanced Catalytic Engineer Research Center of the Ministry of Education, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

Transformation between 2D covalent organic frameworks (COFs) via exchange of molecular building blocks with different symmetries has been realized, which gives rise to the conversion between 2D COFs with distinct pore hierarchy. This type of monomer replacement has expanded the scope of the building-unit-exchange-based COF-to-COF transformation strategy.
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http://dx.doi.org/10.1039/d0cc06536fDOI Listing
December 2020

Promotes the Malignancy Progression of Pancreatic Ductal Carcinoma by Inhibiting the Expression of p27 and PPP2R2A via G1/S Cell Cycle Pathway.

Onco Targets Ther 2020 29;13:11045-11058. Epub 2020 Oct 29.

Department of General Surgery, Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang 110001, People's Republic of China.

Objective: To investigate the effect of on the malignant biological behavior of pancreatic cancer, and to explore the target genes and pathways directly affected by , to provide new therapeutic ideas and targets for the study of the diagnosis and treatment of pancreatic cancer.

Methods: We used qRT-PCR to measure expression quantities. We used cell cycle, CCK-8, clonal formation to verify the change of proliferation capacity of PC cells. We used transwell assay to detect the migration and invasion of PC cells. We used the bioinformatics tool TargetScan (http://www.targetscan.org) to identify the possible target genes of . Immunohistochemistry revealed the clinicopathological significance of PPP2R2A, p27 and in the expression of pancreatic cancer. Western blot was used to detect the expression changes of PPP2R2A, p27 and G1/S cell cycle pathway-related proteins CDK2, cyclinE2 and p21 after transfection of mimics and inhibitors of

Results: According to our study, expression was significantly higher in PC tissues than that in paired normal pancreas, which was associated with PC tumor size (=0.042) and preoperative CA19-9 level (=0.046) of PC patients. Its overexpression promoted PC cell proliferation, invasion and migration following with the p27 and PPP2R2A protein downregulation in Capan-2, PANC-1 and BxPC-3 cells, and vice versa. Bioinformatics analysis and dual-luciferase reporter assay further confirmed that and were direct target genes of . The negative relationship of with p27 and PPP2R2A was also observed in PC tissues.

Conclusion: promotes the proliferation, migration and invasion of pancreatic cancer cells. directly downregulated and and via the G1/S cell cycle pathway to promote the development of pancreatic cancer.
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http://dx.doi.org/10.2147/OTT.S260499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605676PMC
October 2020

Calreticulin promotes EMT in pancreatic cancer via mediating Ca dependent acute and chronic endoplasmic reticulum stress.

J Exp Clin Cancer Res 2020 Oct 7;39(1):209. Epub 2020 Oct 7.

Department of Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, 110001, Liaoning, China.

Background: Our previous study showed that calreticulin (CRT) promoted EGF-induced epithelial-mesenchymal transition (EMT) in pancreatic cancer (PC) via Integrin/EGFR-ERK/MAPK signaling. We next investigated the novel signal pathway and molecular mechanism involving the oncogenic role of CRT in PC.

Methods: We investigated the potential role and mechanism of CRT in regulating intracellular free Ca dependent acute and chronic endoplasmic reticulum stress (ERS)-induced EMT in PC in vitro and vivo.

Results: Thapsigargin (TG) induced acute ERS via increasing intracellular free Ca in PC cells, which was reversed by CRT silencing. Additionally, CRT silencing inhibited TG-induced EMT in vitro by reversing TG-induced changes of the key proteins in EMT signaling (ZO-1, E-cadherin and Slug) and ERK/MAPK signaling (pERK). TG-promoted cell invasion and migration was also rescued by CRT silencing but enhanced by IRE1α silencing (one of the key stressors in unfolded protein response). Meanwhile, CRT was co-immunoprecipitated and co-localized with IRE1α in vitro and its silencing led to the chronic ERS via upregulating IRE1α independent of IRE1-XBP1 axis. Moreover, CRT silencing inhibited IRE1α silencing-promoted EMT, including inhibiting the activation of EMT and ERK/MAPK signaling and the promotion of cell mobility. In vivo, CRT silencing decreased subcutaneous tumor size and distant liver metastasis following with the increase of IRE1α expression. A negative relationship between CRT and IRE1α was also observed in clinical PC samples, which coordinately promoted the advanced clinical stages and poor prognosis of PC patients.

Conclusions: CRT promotes EMT in PC via mediating intracellular free Ca dependent TG-induced acute ERS and IRE1α-mediated chronic ERS via Slug and ERK/MAPK signaling.
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http://dx.doi.org/10.1186/s13046-020-01702-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542892PMC
October 2020

Effect of acupoint therapy combined with spine pinching in patients with menopausal syndrome: a randomized controlled trial.

J Tradit Chin Med 2020 10;40(5):855-862

Physiotherapy Department, Guangzhou Provincial Hospital of Chinese Medicine, Guangzhou 510120, China.

Objective: To assess the efficacy of acupoint therapy combined with spine pinching in patients with menopausal syndrome.

Methods: This is a parallel, randomized, controlled, investigator-blinded trial. A total of 132 participants were randomly assigned to receive either acupoint therapy combined with spine pinching (intervention group) or tibolone therapy alone (control group). The intervention group received acupoint therapy combined with spine pinching three times per week for 4 weeks. The control group received 2.5 mg of tibolone once daily for 4 weeks. The primary outcome was the improved Kupperman score. The WHO quality of life scale was also used. The secondary aim was to identify those who would benefit from acupoint therapy combined with spine pinching based on the levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH).

Results: In the intervention group, the improved Kupperman score was significantly decreased after treatment compared with before treatment. However, there were no differences between the intervention and control groups for any outcome. Changes in the physiology score presented negative outcomes in patients with a low FSH level with increasing body mass index (BMI) (P = 0.0). In contrast, changes in the physiology score presented positive outcomes in patients with a moderate LH level with increasing BMI (P = 0.0). The mean change in the physiology score of patients with a low FSH level and a BMI of ≥25.7 kg/m2 was -7.17 (range -10.94 to-3.40) after adjustments for age and disease duration.

Conclusion: Acupoint therapy combined with spine pinching is effective in treating menopausal syndrome, especially in women with a moderate LH level. However, patients with a low FSH level had a negative outcome after acupoint therapy combined with spine pinching. In addition, patients with a BMI of > 25.7 kg/m2 had a negative outcome after the intervention, regardless of hormone levels.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2020.05.016DOI Listing
October 2020

Corrigendum: gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea.

Int J Syst Evol Microbiol 2020 09;70(9):5185

Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, PR China.

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http://dx.doi.org/10.1099/ijsem.0.004419DOI Listing
September 2020

Conventional and contrast-enhanced ultrasound features in sclerosing adenosis and correlation with pathology.

Clin Hemorheol Microcirc 2021 ;77(2):173-181

Department of Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: To evaluate the efficacy of conventional ultrasound (US) and contrast-enhanced ultrasound (CEUS) in differential diagnosis of sclerosing adenosis (SA) from malignance and investigate the correlated features with pathology.

Methods: We retrospectively enrolled 103 pathologically confirmed SA. All lesions were evaluated with conventional US while 31 lesions with CEUS. Lesions were divided into SA with or without benign lesions (Group 1, n = 81) and SA with malignancy (Group 2, n = 22). Performance of two methods were analyzed. The ultrasonographic characteristics were compared between two groups with Student's t-test for measurement and chi-squared or Fisher's exact test for count data.

Results: There were 22 lesions complicated with malignancy, and the mean age of Group 2 was higher than Group 1 (55.27 vs. 41.57, p < 0.001). The sensitivity, specificity and accuracy of conventional US and CEUS were 95.45%, 46.91%, 57.28% and 100%, 62.5%, 70.97%. Angularity (p < 0.001), spicules (p = 0.023), calcification (p = 0.026) and enlarged scope (p = 0.012) or crab claw-like enhancement (p = 0.008) in CEUS were more frequent detected in SA with malignancy.

Conclusions: Though CEUS showed an improved accuracy, the performance of ultrasound in the diagnosis of SA was limited. Awareness and careful review of the histopathologically related imaging features can be helpful in the diagnosis of SA.
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http://dx.doi.org/10.3233/CH-200943DOI Listing
April 2021

Correction to: Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling.

J Exp Clin Cancer Res 2020 08 24;39(1):167. Epub 2020 Aug 24.

Department of Gastrointestinal Surgery, the First Hospital, China Medical University, Shenyang, 110001, China.

An amendment to this paper has been published and can be accessed via the original article.
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http://dx.doi.org/10.1186/s13046-020-01663-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446150PMC
August 2020

Musashi2 promotes the progression of pancreatic cancer through a novel ISYNA1-p21/ZEB-1 pathway.

J Cell Mol Med 2020 09 11;24(18):10560-10572. Epub 2020 Aug 11.

Department of Gastrointestinal and Hernia and Abdominal Wall Surgery, First Hospital of China Medical University, Shenyang, China.

Our previous studies found overexpression of Musashi2 (MSI2) conduced to the progression and chemoresistance of pancreatic cancer (PC) by negative regulation of Numb and wild type p53 (wtp53). Now, we further investigated the novel signalling involved with MSI2 in PC. We identified inositol-3-phosphate synthase 1 (ISYNA1) as a novel tumour suppressor regulated by MSI2. High MSI2 and low ISYNA1 expression were prevalently observed in 91 PC tissues. ISYNA1 expression was negatively correlated with MSI2 expression, T stage, vascular permeation and poor prognosis in PC patients. What's more, patients expressed high MSI2 and low ISYNA1 level had a significant worse prognosis. And in wtp53 Capan-2 and SW1990 cells, ISYNA1 was downregulated by p53 silencing. ISYNA1 silencing promoted cell proliferation and cell cycle by inhibiting p21 and enhanced cell migration and invasion by upregulating ZEB-1. However, MSI2 silencing upregulated ISYNA1 and p21 but downregulated ZEB-1, which can be rescued by ISYNA1 silencing. Moreover, reduction of cell migration and invasion resulting from MSI2 silencing was significantly reversed by ISYNA1 silencing. In summary, MSI2 facilitates the development of PC through a novel ISYNA1-p21/ZEB-1 pathway, which provides new gene target therapy for PC.
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http://dx.doi.org/10.1111/jcmm.15676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521282PMC
September 2020

Highly compressible and anisotropic lamellar ceramic sponges with superior thermal insulation and acoustic absorption performances.

Nat Commun 2020 Jul 24;11(1):3732. Epub 2020 Jul 24.

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China.

Advanced ceramic sponge materials with temperature-invariant high compressibility are urgently needed as thermal insulators, energy absorbers, catalyst carriers, and high temperature air filters. However, the application of ceramic sponge materials is severely limited due to their complex preparation process. Here, we present a facile method for large-scale fabrication of highly compressible, temperature resistant SiO-AlO composite ceramic sponges by blow spinning and subsequent calcination. We successfully produce anisotropic lamellar ceramic sponges with numerous stacked microfiber layers and density as low as 10 mg cm. The anisotropic lamellar ceramic sponges exhibit high compression fatigue resistance, strain-independent zero Poisson's ratio, robust fire resistance, temperature-invariant compression resilience from -196 to 1000 °C, and excellent thermal insulation with a thermal conductivity as low as 0.034 W m K. In addition, the lamellar structure also endows the ceramic sponges with excellent sound absorption properties, representing a promising alternative to existing thermal insulation and acoustic absorption materials.
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http://dx.doi.org/10.1038/s41467-020-17533-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382455PMC
July 2020

The Effect of L-Carnitine Additive During Maturation on the Vitrification of Pig Oocytes.

Cell Reprogram 2020 08 14;22(4):198-207. Epub 2020 Jul 14.

College of Animal Science and Technology, and Nanjing Agricultural University, Nanjing, China.

Cryopreservation of oocytes/embryos is an important technique for genetic resources; however, the success of vitrification in pig oocytes remained at a relatively lower level due to the high content of lipid droplets (LDs). Considering the positive effect of L-carnitine on the function of LDs, the present study was designed to investigate the effect of the addition of L-carnitine on the vitrification of porcine cumulus cells of complexes (cumulus/oocyte complexes [COCs]). First, COCs were randomly divided into two groups: one group of COCs were commonly maturation (IVM) for 42-46 hours (nonvitrification [NV]), while another group of COCs were IVM with 10 mM L-carnitine (NVL [nonvitrification with L-carnitine addition in IVM]). In addition, random parts of COCs with L-carnitine addition were vitrified (VL [vitrification with L-carnitine addition in IVM]), while vitrification was performed on COCs without L-carnitine used as control group (V). Results showed that the maturation rate of pig oocytes reduced significantly when the vitrification was performed at 16 hours during IVM (VL vs. NVL, 40.09 ± 2.85 vs. 90.76 ± 1.16; V vs. NV, 34.41 ± 2.55 vs. 89.71 ± 1.33,  < 0.01). With the addition of L-carnitine, intracellular LDs were decreased significantly ( < 0.01). However, no difference was observed on the efficiency of vitrification in pig oocytes (VL vs. V, 40.09 ± 2.85 vs. 34.41 ± 2.55,  > 0.05). In addition, not only the reactive oxygen species (ROS) level in pig oocytes with the L-carnitine addition group reduced significantly ( < 0.01), but also the expression of gene was improved ( < 0.05). In conclusion, results demonstrated that although no difference could be observed on pig COC vitrification, the LDs and ROS level in pig oocytes could be modified by the addition of L-carnitine, which might be helpful for further development.
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http://dx.doi.org/10.1089/cell.2020.0014DOI Listing
August 2020

Reveal a hidden highly toxic substance in biochar to support its effective elimination strategy.

J Hazard Mater 2020 11 28;399:123055. Epub 2020 May 28.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai 200438, China. Electronic address:

With the aim to develop optimized biochar with minimal contaminants, it is important significance to broaden the understanding of biochar. Here, we disclose for the first time, a highly toxic substance (metal cyanide, MCN, such as KCN or NaCN) in biochar. The cyanide ion (CN) content in biochar can be up to 85,870 mg/kg, which is determined by the inherent metal content and type in the biomass with K and Na increasing and Ca, Mg and Fe decreasing its formation. Density functional theory (DFT) analysis shows that unstable alkali oxygen-containing metal salts such as KCO can induce an N rearrangement reaction to produce for example, KOCN. The strong reducing character of the carbon matrix further converts KOCN to KCN, thus resulting biochar with high risk. However, the stable Mg, Ca and Fe salts in biomass cannot induce an N rearrangement reaction due to their high binding energies. We therefore propose that high valent metal chloride salts such as FeCl and MgCl could be used to inhibit the production of cyanide via metal interactive reaction. These findings open a new point of view on the potential risk of biochar and provide a mitigation solution for biochar's sustainable application.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123055DOI Listing
November 2020

A Foldable All-Ceramic Air Filter Paper with High Efficiency and High-Temperature Resistance.

Nano Lett 2020 Jul 4;20(7):4993-5000. Epub 2020 Jun 4.

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Advanced filter materials with high efficiency, low-pressure drop, and high-temperature resistance are urgently needed in the field of high-temperature gas filtration. Here, an AlO-stabilized ZrO (ASZ) submicron fiber air filter paper with excellent flexibility and thermal stability (up to 1100 °C) is developed using a cost-effective, scalable solution blow spinning method and subsequent calcination. The ASZ papers demonstrate excellent flexibility and foldability, which can be attributed to the tetragonal phase and small crystallite size of the ASZ fibers due to the presence of AlO. In addition, the ASZ papers with an areal density of 56 mg cm show a high filtration efficiency (99.56%) and a low-pressure drop (108 Pa) for 15-615 nm NaCl particles at an airflow velocity of 5.4 cm s. We envision that the foldable all-ceramic air filter material will provide a solution for the removal of particulate matter from the high-temperature exhaust gases.
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http://dx.doi.org/10.1021/acs.nanolett.0c01107DOI Listing
July 2020

N-rich hydrochar derived from organic solvent as reaction medium generates toxic N-containing mineral in its pyrochar.

Sci Total Environ 2020 Aug 27;729:138970. Epub 2020 Apr 27.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Low-grade hydrochar is often thermally activated to produce pyrochar. However, the interaction between inherent metals and N element during hydrochar activation process has never been considered. Present study revealed that a highly toxic N-containing mineral (MCN, M: metal) can be formed in N-rich hydrochar-based pyrochar via N rearrangement and subsequent carbothermal reactions. This atypical hydrochar is mainly produced from organic wastes with KCO or NaCO (such as food waste) in an organic solvent medium. The CN concentration of studied pyrochar can reach to 9807 mg/kg, which is mainly determined by content and type of metal in hydrochar. The low N conversion rate (<4.2%) indicates that formation of MCN is independent of N content in hydrochar. Essentially, formation of MCN can be significantly inhibited by FeCl via the anionic interactions between Fe and K salt. This discovery can likely offer a new guide for the application of N-rich hydrochar-based pyrochar.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138970DOI Listing
August 2020

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus.

Ann Clin Microbiol Antimicrob 2020 Mar 26;19(1):10. Epub 2020 Mar 26.

Department of Orthopedics, Southwest Hospital, Army Medical University, Gaotanyan Main Street 30#, District Shapingba, Chongqing, China.

Background: Staphylococcus aureus is a primary pathogen of orthopedic infections. By mediating antimicrobial resistance, S. aureus biofilm plays an important role in the recalcitrance of orthopedic infections, especially for the intractable osteomyelitis (OM). This study investigated the relationship between biofilm production and various genetic or phenotypic characteristics among orthopedic S. aureus strains.

Methods: A total of 137 orthopedic S. aureus isolates were enrolled and divided into OM and non-OM groups. Biofilm production was evaluated using the crystal violet assay. Genetic and phenotypic characteristics including MRSA identification, MLST and spa typing, carriage of virulence genes, drug resistance, and patients' inflammatory responses indicators were characterized. The relationship between biofilm production and above-mentioned features was respectively analyzed among all isolates and compared between OM and non-OM isolates.

Results: Biofilm production presented no significant difference between OM (including 9 MRSA isolates) and non-OM (including 21 MRSA isolates) strains. We found that ST88, t377 and ST630-MSSA-t377 strains produced very strong biofilms, while MLST types of ST15, ST25, ST398, ST5, ST59 and spa types of t002, t2325, t437 tended to produce weaker biofilms. Strains with the following profiles produced stronger biofilms: fib(+)-hlgv(+)-lukED(+)-sei(-)-sem(-)-seo(-) for all isolates, sei(-)-sem(-)-seo(-) for OM isolates, and cna (+)-fib (+)-hlgv (+)-lukED (+)-seb(-)-sed(-) for non-OM isolates. In addition, not any single drug resistance was found to be related to biofilm production. We also observed that, among OM patients, strains with stronger biofilms caused weaker inflammatory responses.

Conclusion: Some genetic or phenotypic characteristics of orthopedic strains were associated with biofilm production, and this association could be different among OM and non-OM strains. The results are of great significance for better understanding, evaluating and managing different kinds of biofilm-associated orthopedic infections, and provide potential targets for biofilm clearance.
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http://dx.doi.org/10.1186/s12941-020-00352-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099788PMC
March 2020

sp. nov., isolated from southern Okinawa Trough sediment and emended description of the family .

Int J Syst Evol Microbiol 2020 Apr 20;70(4):2531-2536. Epub 2020 Feb 20.

Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, PR China.

A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, long-rod-shaped and non-flagellated bacterial strain, designated T3-2 S1-C, was isolated from a sediment sample collected at the Okinawa Trough. Phylogenetic analyses of 16S rRNA gene sequences and the whole genome revealed that strain T3-2 S1-C was a member of the family and exhibited less than 95.1 % sequence similarities to the closely related type strains of the family . Optimal growth occurred at pH 7.0, 28 °C and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain T3-2 S1-C was identified as menaquinone-7 (MK-7) and the predominant fatty acids (>10 %) were iso-C (38.9 %) and anteiso-C (11.6 %). The major polar lipids were one phosphatidylethanolamine, one phosphatidylmonomethylethanolamine, one aminolipids, two unidentified lipids and two unidentified phospholipids. The DNA G+C content of strain T3-2 S1-C was 35.7 mol%. On the basis of the results of polyphasic analyses, strain T3-2 S1-C is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3-2 S1-C (=KCTC 15505=MCCC 1K01617).
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http://dx.doi.org/10.1099/ijsem.0.004066DOI Listing
April 2020

Nanocomposites membranes from cellulose nanofibers, SiO and carboxymethyl cellulose with improved properties.

Carbohydr Polym 2020 Apr 31;233:115818. Epub 2019 Dec 31.

Beijing Engineering Research Centre of Cellulose and Its Derivatives, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address:

The binary nanocomposites blended by carboxymethyl cellulose (CMC) and SiO nanoparticles were constructed to prepare the films with superior thermal stability and flame retardant properties. The incorporation of cellulose nanofibers(CNFs) and SiO nanoparticles were followed to prepare ternary nanocomposite films exhibiting excellent mechanical properties. The mechanism and chemical reaction of the thermal decomposition for the CMC/SiO composite membrane were proposed, which showed that the mass residuals were NaCO, SiO and NaSiO, NaCO when the content of the SiO nanoparticles was lowered and higher than 9.6 %, respectively. Compared with the pure CMC, micro combustion calorimeter (MCC) showed that the total heat release (THR) and the peak heat release rate (PHRR) both decreased from 6.4 kJ/g to 5.8 kJ/g, 134 w/g to 27 w/g, respectively. Moreover, mechanical properties of CMC/CNFs/SiO membrane showed that the toughness and rigidity of the nanocomposites increased by 56.0 % and 63.0 % on the basis of CMC, respectively.
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http://dx.doi.org/10.1016/j.carbpol.2019.115818DOI Listing
April 2020

An antibiotic cement-coated locking plate as a temporary fixation for treatment of infected bone defects: a new method of stabilization.

J Orthop Surg Res 2020 Feb 11;15(1):44. Epub 2020 Feb 11.

Department of Orthopedics, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, People's Republic of China.

Background: The induced membrane technique has achieved good clinical results in the treatment of infected bone defects, and external fixation is the main method, but it causes inconvenience and complications in patients. In this study, our objective was to investigate the outcomes of using an antibiotic cement-coated locking plate as a temporary internal fixation in the first stage of the surgical induced membrane technique for treating extremities with infected bone defects.

Methods: We retrospectively analysed patients with lower extremity infected bone defects in our department between January 2013 and December 2017. All patients were treated with the induced membrane technique. In the first stage, the defects were stabilized with an antibiotic cement-coated locking plate as a temporary fixation after debridement, and polymethyl methacrylate cement was implanted to induce the formation of a membrane. In the second stage, bone grafting rebuilt the bone defects after infection control, and the temporary fixation was changed to a stronger fixation.

Results: A total of 183 patients were enrolled, with an average follow-up duration of 32.0 (12-66) months. There were 154 males and 29 females with an average age of 42.8 (10-68) years. The infection sites included 81 femurs, 100 tibias and 2 fibulas. After the first stage of treatment (infection control), 16 (8.7%) patients had recurrence of infection. In terms of the incidence of complications, 4 patients had poor wound healing, 2 patients had fixation failure and 1 patient had femoral fracture due to a fall. After the second stage of treatment (bone reconstruction), there were 24 (13.1%) recurrences of infection, with a mean time of 9.9 months (range 0.5 to 36). Among them, 18 patients underwent bone grafting after re-debridement, 6 received permanent placement of antibiotic bone cement after debridement and 2 patients refused further treatment and chose amputation. Bone healing was achieved in 175 (95.9%) patients at the last follow-up, and the average time to bone union was 5.4 (4-12) months.

Conclusions: Antibiotic cement-coated locking plates have good clinical effects in the control of bone infection, but attention must be paid to the possible difficulty of skin coverage when applied in calves.
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http://dx.doi.org/10.1186/s13018-020-1574-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7014650PMC
February 2020

gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea.

Int J Syst Evol Microbiol 2020 03 21;70(3):1623-1629. Epub 2020 Jan 21.

Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.

Blue holes are unique geomorphological units characterized by steep redox and biogeochemical gradients. Yongle Blue Hole is located on the largest atoll (Yongle Atoll) of the western Xisha Islands in the South China Sea. A Gram-stain-negative, facultatively anaerobic, non-motile, non-flagellated marine bacterium with creamy white colonies, designated JC036, was isolated from Yongle Blue Hole. Cells were short-rod-shaped and catalase-negative. 16S rRNA gene sequence analysis showed that sequence similarities were lower than 91.6 % against all validly named species in the family ; a reconstructed phylogenetic tree indicated that strain JC036 formed a lineage with strains in the family . Growth occurred at 4-37 °C (optimum, 28 °C), at pH 5.0-9.0 (optimum, 7.0) and in the presence of 2-6 % (w/v) NaCl (optimum, 3 %). The prevalent isoprenoid quinone of strain JC036 was menaquinone-7 (MK-7). Iso-C and iso-C 3-OH were the predominant fatty acids. The major polar lipids included a phospholipid, phosphatidylethanolamine, an aminophospholipid and four unidentified lipids. The genomic DNA G+C content of strain JC036 was 37.8 mol%. Based on physiological and biochemical characteristics and whole genome comparisons, we propose a new genus and species, gen. nov., sp. nov., within the family . The type strain of is JC036 (=JCM 33128=MCCC 1K03579). From this study, a deeper understanding of the community of the microorganism and their roles in biogeochemical cycles, especially anaerobic bacteria, is provided.
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http://dx.doi.org/10.1099/ijsem.0.003948DOI Listing
March 2020

Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling.

J Exp Clin Cancer Res 2020 Jan 17;39(1):16. Epub 2020 Jan 17.

Department of Gastrointestinal Surgery, the First Hospital, China Medical University, Shenyang, 110001, China.

Background: Our previous study showed Musashi2 (MSI2) promoted chemotherapy resistance and pernicious biology of pancreatic cancer (PC) by down-regulating Numb and p53. We further explored the novel molecular mechanism involving its oncogenic role in PC development.

Methods: We investigated the potential role and mechanism of MSI2 in EGF-induced EMT in PC in vitro and vivo.

Results: EGF enhanced EGFR (epidermal growth factor receptor) phosphorylation, induced EMT and activated ZEB1-ERK/MAPK signaling in 2 PC cells. However, MSI2 silencing reversed EGF stimulated function, including inhibiting EGF-promoted EMT-like cell morphology and EGF-enhanced cell invasion and migration. Meanwhile, MSI2 silencing inhibited EGF-enhanced EGFR phosphorylation at tyrosine 1068 and reversed EGF-induced change of the key proteins in EMT and ZEB1-ERK/MAPK signaling (ZEB1, E-cad, ZO-1, β-catenin, pERK and c-Myc). Additionally, MSI2 was co-stained and co-immunoprecipitated with ZEB1, pERK and c-Myc in PC cells by IF and co-IP, implying a close interaction between them. In vivo, MSI2 silencing inhibited pancreatic tumor size in situ and distant liver metastases. A close relationship of MSI2 with EMT and ZEB1-ERK/MAPK signaling were also observed in vivo and human PC samples, which coordinately promoted the poor prognosis of PC patients.

Conclusions: MSI2 promotes EGF-induced EMT in PC via ZEB1-ERK/MAPK signaling.
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http://dx.doi.org/10.1186/s13046-020-1521-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6967093PMC
January 2020