Publications by authors named "Chao Feng"

447 Publications

Porous MoWN/[email protected] Nano-octahedrons synthesized via confined carburization and vapor deposition in MOFs as efficient trifunctional electrocatalysts for oxygen reversible catalysis and hydrogen production in the same electrolyte.

J Colloid Interface Sci 2021 May 26;601:626-639. Epub 2021 May 26.

Key Laboratory of Oil & Gas Fine Chemicals Ministry of Education & Xinjiang Uyghur Autonomous Region, Xinjiang University, Urumqi 830046, Xinjiang, China. Electronic address:

We used a simple MOFs-assisted synthesis strategy based on the encapsulation and in-situ carburizing reaction of Cu-based metallic organic frameworks (NENU-5) to synthesize porous nano-octahedral materials, MoWN/[email protected] (T = 700, 800, and 900). Together with the vapor deposition strategy, the volatile nitrogen species from the pyrolysis of dicyandiamide were captured by the nano-octahedral materials, and formed tungsten-molybdenum-based carbonitride nanocrystals encapsulated in nitrogen-doped carbon. The porous nano-octahedron has a unique heterostructure composed of MoN/MoC/WN/WC. The representative MoWN/[email protected] showed trifunctional electrocatalytic activity in oxygen reduction reaction/oxygen evolution reaction/hydrogen evolution reaction (ORR/OER/HER) in an alkaline medium (0.1 M KOH). The total oxygen electrode activity index ΔE = 0.795 V (vs. RHE) was found in OER/ORR, and the material also exhibits excellent HER performance. The minimum potential of -0.17 V (vs. RHE) was provided at a current density of -10 mA cm. MoWN/[email protected] showed excellent cycle stability and durability in ORR/OER/HER with the same electrolyte (0.1 M KOH). More importantly, MoWN/[email protected] could be used to construct high-performance zinc-air batteries and sued for driving electrocatalytic water splitting in a self-powered manner. The successful preparation of the materials indicate that the synthetic strategy provides new reference ideas for developing functional materials with high catalytic properties for various applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.133DOI Listing
May 2021

A composite sponge based on alkylated chitosan and diatom-biosilica for rapid hemostasis.

Int J Biol Macromol 2021 May 31;182:2097-2107. Epub 2021 May 31.

College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China. Electronic address:

Rapid control of bleeding is of great significance in military trauma and traffic accidents. In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control. Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity). The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration. AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg). All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.123DOI Listing
May 2021

Thrombin immobilized polydopamine-diatom biosilica for effective hemorrhage control.

Biomater Sci 2021 Jun 1. Epub 2021 Jun 1.

College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China.

In this study, an efficient composite hemostatic material (DA-diatom-T) was prepared, using a polydopamine layer as a linker to immobilize thrombin on the surface of diatom biosilica. DA-diatom-T retained the porous structure of the diatom with high water absorption capacity, which can absorb 31 times its own weight of water. The thrombin activity of DA-diatom-T was as high as 5.81 U mg-1 that could be maintained at 67% after 30 days at room temperature. DA-diatom-T exhibited non-toxicity to mouse fibroblast cell lines, favorable hemocompatibility and fast procoagulant ability. DA-diatom-T could promote the initiation of the coagulation process and increase platelet activity and blood clot strength to form a physical barrier at the wound. In an in vivo study, DA-diatom-T could significantly reduce the clotting time and reduce the bleeding volume. The above results showed that DA-diatom-T had potential as a new hemostatic material.
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http://dx.doi.org/10.1039/d0bm02116dDOI Listing
June 2021

CircMYLK promotes the growth, migration, invasion, and survival of bladder cancer cells by upregulating CCND3 level via competitively binding to miR-34a.

Drug Dev Res 2021 May 30. Epub 2021 May 30.

Department of Urology, Xinchang County People's Hospital, Xinchang, China.

Bladder cancer is one of the most common types of urothelial carcinoma with a rising incidence rate worldwide. Circular RNAs (circRNAs) are involved in the development of numerous cancers, including bladder cancer. We aimed to uncover the role and associated mechanism of circMYLK in bladder cancer. The expression levels of circMYLK, miRNA-34a (miR-34a) and Cyclin D3 (CCND3) mRNA were investigated using real-time quantitative polymerase chain reaction. The protein level of CCND3 was investigated using western blot. In functional assays, flow cytometry assays were utilized for cell cycle analysis and cell apoptosis analysis. Transwell assays were used for cell migration and invasion analysis. Caspase-3 activity was examined to monitor cell apoptosis. The putative relationship between miR-34a and circMYLK or CCND3 was validated by dual-luciferase reporter assay and RNA immunoprecipitation assay. CircMYLK was highly expressed in bladder cancer tissues and cells. CircMYLK downregulation inhibited bladder cancer cell migration and invasion, and promoted cancer cell apoptosis and cell cycle arrest. MiR-34a, a target of circMYLK, was downregulated in bladder cancer tissues and cells. MiR-34a inhibition reversed the effects of circMYLK downregulation and then recovered bladder cell malignant behaviors. Further analysis showed that CCND3 was a downstream target of miR-34a, and CCND3 was upregulated in bladder cancer tissues and cells. MiR-34a overexpression blocked bladder cancer cell migration and invasion, and induced cell apoptosis and cycle arrest, while these effects were abolished by CCND3 overexpression. CircMYLK contributed to the malignant development of bladder cancer cells partly through the miR-34a/CCND3 regulatory network, showing the significance of circMYLK in bladder cancer pathogenesis.
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http://dx.doi.org/10.1002/ddr.21835DOI Listing
May 2021

Knl1 participates in spindle assembly checkpoint signaling in maize.

Proc Natl Acad Sci U S A 2021 May;118(20)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, 100101 Beijing, China;

The Knl1-Mis12-Ndc80 (KMN) network is an essential component of the kinetochore-microtubule attachment interface, which is required for genomic stability in eukaryotes. However, little is known about plant Knl1 proteins because of their complex evolutionary history. Here, we cloned the homolog from maize () and confirmed it as a constitutive central kinetochore component. Functional assays demonstrated their conserved role in chromosomal congression and segregation during nuclear division, thus causing defective cell division during kernel development when Knl1 transcript was depleted. A 145 aa region in the middle of maize Knl1, that did not involve the MELT repeats, was associated with the interaction of spindle assembly checkpoint (SAC) components Bub1/Mad3 family proteins 1 and 2 (Bmf1/2) but not with the Bmf3 protein. They may form a helical conformation with a hydrophobic interface with the TPR domain of Bmf1/2, which is similar to that of vertebrates. However, this region detected in monocots shows extensive divergence in eudicots, suggesting that distinct modes of the SAC to kinetochore connection are present within plant lineages. These findings elucidate the conserved role of the KMN network in cell division and a striking dynamic of evolutionary patterns in the SAC signaling and kinetochore network.
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http://dx.doi.org/10.1073/pnas.2022357118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157932PMC
May 2021

A coherent harmonic generation method for producing femtosecond coherent radiation in a laser plasma accelerator based light source.

J Synchrotron Radiat 2021 May 22;28(Pt 3):669-680. Epub 2021 Mar 22.

China Spallation Neutron Source, Institute of High Energy Physics, Chinese Academy of Sciences, Dongguan, Guangdong 523803, People's Republic of China.

The electron beam generated in laser plasma accelerators (LPAs) has two main initial weaknesses - a large beam divergence (up to a few milliradians) and a few percent level energy spread. They reduce the beam brightness and worsen the coherence of the LPA-based light source. To achieve fully coherent radiation, several methods have been proposed for generating strong microbunching on LPA beams. In these methods, a seed laser is used to induce an angular modulation into the electron beam, and the angular modulation is converted into a strong density modulation through a beamline with nonzero longitudinal position and transverse angle coupling. In this paper, an alternative method to generate microbunching into the LPA beam by using a seed laser that induces an energy modulation and transverse-longitudinal coupling beamlines that convert the energy modulation into strong density modulation is proposed. Compared with the angular modulation methods, the proposed method can use more than one order of magnitude lower seed laser power to achieve similar radiation performance. Simulations show that with the proposed method a coherent pulse of a few microjoules pulse energy and femtosecond duration can be generated with a typical LPA beam.
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http://dx.doi.org/10.1107/S1600577521002745DOI Listing
May 2021

Visible-Light-Induced Meerwein Fluoroarylation of Styrenes.

Org Lett 2021 May 5;23(10):4040-4044. Epub 2021 May 5.

Technical Institute of Fluorochemistry (TIF), Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, P. R. China.

An unprecedented approach for assembling a broad range of 1,2-diarylethane derivatives with fluorine-containing fully substituted carbon centers was developed. The protocol features straightforward operation, proceeds under metal-free condition, and accommodates a large variety of synthetically useful functionalities. The critical aspect to the success of this novel transformation lies in using aryldiazonium salts as both aryl radical progenitor and also as single electron acceptor which elegantly enables a radical-polar crossover manifold.
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http://dx.doi.org/10.1021/acs.orglett.1c01249DOI Listing
May 2021

FVE promotes RNA-directed DNA methylation by facilitating the association of RNA polymerase V with chromatin.

Plant J 2021 May 3. Epub 2021 May 3.

National Institute of Biological Sciences, Beijing, 102206, China.

Association of RNA polymerase V (Pol V) with chromatin is a critical step for RNA- directed DNA methylation (RdDM) in plants. Although the methylated DNA-binding proteins SUVH2 and SUVH9 and the chromatin remodeler-containing complex DRD1-DMS3-RDM1 are known to be required for the association of Pol V with chromatin, the molecular mechanisms underlying the association of Pol V with different chromatin environments remain largely unknown. Here we found that SUVH9 interacts with FVE, a homolog of the mammalian retinoblastoma-associated protein, which has been previously identified as a shared subunit of the histone deacetylase complex and the polycomb-type histone H3K27 trimethyltransferase complex. We demonstrated that FVE facilitates the association of Pol V with chromatin and thus contributes to DNA methylation at a substantial subset of RdDM target loci. Compared with FVE-independent RdDM target loci, FVE-dependent RdDM target loci are more abundant in gene-rich chromosome arms than in pericentromeric heterochromatin regions. This study contributes to our understanding of how the association of Pol V with chromatin is regulated in different chromatin environments.
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http://dx.doi.org/10.1111/tpj.15302DOI Listing
May 2021

Tübingen hip flexion splint more successful than Pavlik harness for decentred hips after the age of three months.

Bone Joint J 2021 May;103-B(5):991-998

Department of Pediatric Orthopaedics, Beijing Jishuitan Hospital, Beijing, China.

Aims: The objective of this study was to evaluate the clinical and radiological outcomes of patients younger than six months of age with developmental dysplasia of the hip (DDH) managed by either a Pavlik harness or Tübingen hip flexion splint.

Methods: Records of 251 consecutive infants with a mean age of 89 days (SD 47), diagnosed with DDH between January 2015 and December 2018, were retrospectively reviewed. Inclusion criteria for patients with DDH were: younger than 180 days at the time of diagnosis; ultrasound Graf classification of IIc or greater; treatment by Pavlik harness or Tübingen splint; and no prior treatment history. All patients underwent hip ultrasound every seven days during the first three weeks of treatment and subsequently every three to four weeks until completion of treatment. If no signs of improvement were found after three weeks, the Pavlik harness or Tübingen splint was discontinued. Statistical analysis was performed.

Results: The study included 251 patients with Graf grades IIc to IV in 18 males and 233 females with DDH. Mean follow-up time was 22 months (SD 10). A total of 116 hips were graded as Graf IIc (39.1%), nine as grade D (3.0%), 100 as grade III (33.7%), and 72 as grade IV (24.2%). There were 109 patients (128 hips) in the Pavlik group and 142 patients (169 hips) in the Tübingen group (p = 0.227). The Tübingen group showed a 69.8% success rate in Graf III and Graf IV hips while the success rate was significantly lower in the Pavlik group, 53.9% (p = 0.033). For infants older than three months of age, the Tübingen group showed a 71.4% success rate, and the Pavlik group a 54.4% success rate (p = 0.047).

Conclusion: The Tübingen splint should be the preferred treatment option for children older than three months, and for those with severe forms of DDH such as Graf grade III and IV, who are younger than six months at time of diagnosis. The Tübingen hip flexion splint is a valid alternative to the Pavlik harness for older infants and those with more severe DDH. Cite this article:  2021;103-B(5):991-998.
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http://dx.doi.org/10.1302/0301-620X.103B5.BJJ-2020-1946.R1DOI Listing
May 2021

Increased inequalities of per capita CO emissions in China.

Sci Rep 2021 Apr 30;11(1):9358. Epub 2021 Apr 30.

School of Economics and Business Administration, Chongqing University, Chongqing, 400030, China.

Designing inter-regional and inter-provincial responsibility-sharing mechanisms for climate change mitigation requires the knowledge of carbon distributions. This study is the first to use a two-sector (i.e., productive and household sectors) inequality decomposition approach to examine the regional, provincial, and national inequalities of per capita CO emissions (CPC) in China, as well as their determinants. We show that the CPC inequality index in China increased from 1.1364 in 2000 to 2.3688 in 2017, with the productive sector accounting for 91.42% of this expansion and households responsible for the rest. The production-side per capita output level, energy efficiency, energy structure, and industrial structure explain 69.01%, 12.81%, 5.57%, and 4.03% of these inequalities, respectively. Further, the household per capita energy consumption and energy structure explain only 8.12% and 0.46%, respectively. Therefore, future responsibility-sharing mechanisms for climate mitigation need to be formulated taking mainly the productive sector into account.
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http://dx.doi.org/10.1038/s41598-021-88736-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087767PMC
April 2021

The heterogeneous impact of environmental regulation on urban green scale economy: an empirical analysis based on city-level panel data in China.

Authors:
Ying Tian Chao Feng

Environ Sci Pollut Res Int 2021 Apr 28. Epub 2021 Apr 28.

School of Economics and Business Administration, Chongqing University, Chongqing, 400030, China.

With continued expansions of scale, the Chinese cities are facing unprecedented economic, environmental, and energy sustainability challenges. The aim of this paper is to examine the heterogeneous impact of environmental regulation (ER) on urban green scale economy (GSEC), by combining the global Malmquist index approach, spatial econometric model, panel quantile regression model (QRPD), and the city-level panel data from 2004 to 2018. The estimation results show that (1) from 2004 to 2018, the growth rate of GSEC fluctuates sharply, and the values of GSEC are less than 1 in many years. This indicates that GSEC is far from efficient and needs to be improved in the future; (2) the results of the spatial econometric model show that GSEC has a U-shaped relationship with the level of ER. Furthermore, the estimation results of QRPD verify the above conclusions and show that there is distinct heterogeneity of ER on GSEC at different quantiles and regional cities; (3) the results further indicate that other vital control variables have a significant and heterogeneous effect on GSEC. Therefore, the focus of future work should be adapted to local conditions. Specifically, in the cities with lower-level GSEC, the policymakers should give top priority to break through the U-shaped inflection point as soon as possible and help enterprises to achieve pollution reduction by technical and financial subsidies. While in the cities with higher-level GSEC, the government should further strengthen its ER and encourage competition among enterprises by internal technological innovation. In addition, the government should try to avoid the negative effect of the "resource curse" trap, excessive financial leverage, and "race-to-the-bottom" phenomenon.
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http://dx.doi.org/10.1007/s11356-021-14031-0DOI Listing
April 2021

Synthesis of oxadiazole-2-oxide derivatives as potential drug candidates for schistosomiasis targeting SjTGR.

Parasit Vectors 2021 Apr 26;14(1):225. Epub 2021 Apr 26.

School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.

Background: Schistosomiasis is a chronic parasitic disease that affects millions of people's health worldwide. Because of the increasing drug resistance to praziquantel (PZQ), which is the primary drug for schistosomiasis, developing new drugs to treat schistosomiasis is crucial. Oxadiazole-2-oxides have been identified as potential anti-schistosomiasis reagents targeting thioredoxin glutathione reductase (TGR).

Methods: In this work, one of the oxadiazole-2-oxides derivatives furoxan was used as the lead compound to exploit a series of novel furoxan derivatives for studying inhibitory activity against both recombinant Schistosoma japonicum TGR containing selenium (rSjTGR-Sec) and soluble worm antigen protein (SWAP) containing wild-type Schistosoma japonicum TGR (wtSjTGR), in order to develop a new leading compound for schistosomiasis. Thirty-nine novel derivatives were prepared to test their activity toward both enzymes. The docking method was used to detect the binding site between the active molecule and SjTGR. The structure-activity relationship (SAR) of these novel furoxan derivatives was preliminarily analyzed.

Results: It was found that several new derivatives, including compounds 6a-6d, 9ab, 9bd and 9be, demonstrated greater activity toward rSjTGR-Sec or SWAP containing wtSjTGR than did furoxan. Interestingly, all intermediates bearing hydroxy (6a-6d) showed excellent inhibitory activity against both enzymes. In particular, compound 6d with trifluoromethyl on a pyridine ring was found to have much higher inhibition toward both rSjTGR-Sec (half-maximal inhibitory concentration, IC,7.5nM) and SWAP containing wtSjTGR (IC 55.8nM) than furoxan. Additionally, the docking method identified the possible matching sites between 6d and Schistosoma japonicum TGR (SjTGR), which theoretically lends support to the inhibitory activity of 6d.

Conclusion: The data obtained herein showed that 6d with trifluoromethyl on a pyridine ring could be a valuable leading compound for further study.
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http://dx.doi.org/10.1186/s13071-021-04634-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074465PMC
April 2021

Colossal Anomalous Hall Effect in Ferromagnetic van der Waals CrTe.

ACS Nano 2021 Apr 21. Epub 2021 Apr 21.

Hefei National Research Center for Physical Sciences at the Microscale, International Centre for Quantum Design of Functional Materials, Department of Materials Science & Engineering, CAS Key Lab of Materials for Energy Conversion, University of Science and Technology of China, Hefei, Anhui 230026, China.

van der Waals crystals exhibit excellent material performance when exfoliated to few-atomic-layer thickness. In contrast, the van der Waals thin films more than 10 nm thick are believed to show bulk properties, in which outstanding material performance is rarely found. Here we report the largest anomalous Hall conductivity observed so far in a 170 nm van der Waals ferromagnetic 1-CrTe flake, which reaches 67,000 Ω cm. Such a colossal anomalous Hall conductivity in 1-CrTe is dominated by the extrinsic skew scattering process rather than the intrinsic Berry phase effect, as evidenced by the linear relation between the anomalous Hall conductivity and the longitudinal conductivity. Defying the dilemma of mutually exclusive large anomalous Hall angle and high electric conductivity for most ferromagnets, 1-CrTe achieves both in a thin film sample. Considering the shared physics of the anomalous Hall effect and the spin Hall effect, our finding offers a guideline for searching large spin Hall materials of high conductivity which may overcome the bottleneck of overheating in spintronics devices.
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http://dx.doi.org/10.1021/acsnano.1c00488DOI Listing
April 2021

What drives the decoupling between economic growth and energy-related CO emissions in China's agricultural sector?

Authors:
Ying Liu Chao Feng

Environ Sci Pollut Res Int 2021 Apr 13. Epub 2021 Apr 13.

School of Economics and Business Administration, Chongqing University, Chongqing, 400030, China.

Many studies have shown that the rapid agricultural mechanization development in China led to substantial energy consumption and CO emission growth. To better explain the mechanism behind the decoupling between economic growth and CO emissions, this paper extends the logarithmic mean Divisia index (LMDI) and production-theoretical decomposition (PDA) considering agricultural decoupling from both structural and technical perspectives. The results reveal that (1) China's agricultural decoupling performance was not ideal. Investment and investment efficiency were the most important factors influencing the decoupling status. The main decoupling obstacle was a higher investment in productivity rather than in energy conservation and carbon reduction. (2) The decoupling status and investment orientation of decoupling efforts among regions were different. Strong negative decoupling statuses frequently occurred in the eastern region, whose main disadvantage was high potential energy intensity. The decoupling status of the central region exhibited expansive features. The decoupling key is to invest more in energy-saving technology rather than in production. The western region changed from weak decoupling to expansive negative decoupling. Both output technology and energy-related factors should be the main investment targets. (3) Weak decoupling and expansive negative decoupling were the most common statuses among provinces. The influence mechanism of drivers exhibited a high spatial heterogeneity at the provincial level. Therefore, the study offered a convincing basis for local governments to formulate low-carbon agricultural development policies by identifying the main decoupling drivers.
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http://dx.doi.org/10.1007/s11356-021-13508-2DOI Listing
April 2021

Trans-olecranon fracture-dislocation of the elbow in children.

Int Orthop 2021 Apr 12. Epub 2021 Apr 12.

Department of Pediatric Orthopedics, Beijing Jishuitan Hospital, Beijing, 100035, China.

Objectives: Trans-olecranon fracture-dislocations are rare in children. To our knowledge, only 12 cases have been described in children till now and the treatment strategy for this injury in children remains unclear. To provide a clear clinical description and more accurate treatment options, we retrospectively reviewed cases with this kind of injury in our institution.

Methods: From 2002 to 2019, eleven cases diagnosed with trans-olecranon fracture-dislocation of the elbow were identified, and their medical charts and radiographs were obtained. All patients underwent open reduction and internal fixation through a posterior approach. At the most recent follow-up visit, all patients were evaluated clinically using the Mayo Elbow Performance Score (MEPS).

Results: The mean follow-up was 22 months (range, 6-42 months). All injuries were unilateral, and there were nine males and two females. The mean age at injury was nine years (range, 4-13 years), and the mean time from injury to surgery was 16.6 days (range, 2-60 days). According to Tiemdjo classification, there was one case with type I injury, one case with type II, six cases with type III, and three cases with type IV. According to the MEPS criteria, the outcomes were excellent in five cases, good in two cases, fair in one case, and poor in three cases. Four patients were delayed cases, who underwent surgery two weeks after injury. The average operation time was significantly longer in four children sustaining delayed surgery (140 ± 43 min, vs. 50 ± 12 min, p < 0.001).

Conclusion: To our knowledge, this is the largest sample size reported to date. We recommend open reduction and internal fixation, using either plates or tension-band techniques, depending on the injury pattern. In addition, we emphasize that early operation could achieve good clinical outcomes.
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http://dx.doi.org/10.1007/s00264-021-05010-8DOI Listing
April 2021

Effects of dietary chromium picolinate supplementation on broiler growth performance: A meta-analysis.

PLoS One 2021 6;16(4):e0249527. Epub 2021 Apr 6.

Department of Life Science, Hulunbuir University, Hulunbuir, Inner Mongolia Autonomous Region, China.

Objective: A meta-analysis was conducted to assess the effects of dietary chromium picolinate (CrPic) supplementation on broiler growth performance and to determine whether such effects are regulated by broiler strains, sex, environmental stress, or contextual factors including study area and years.

Methods: Eligible studies were identified by searching the Web of Science, Springer, Elsevier, ScienceDirect, Taylor & Francis Online databases. Weighted average differences with corresponding 95% confidence intervals were computed with a random-effects model. We performed subgroup analysis stratified by study area, published years, broiler strains and sex, and environmental stress. Publication bias was assessed with Egger's test method. A total of 15 studies eligible for inclusion.

Results: The results indicated that CrPic supplementation significantly improved broiler growth performance and subgroup analysis confirmed this conclusion. We also found that Ross 308 or male broilers might be more sensitive to CrPic supplementation and showed better growth performance. A model was used to obtain the amount of chromium addition under the optimal growth performance, which suggested that the maximum value of average daily gain (ADG) was reached when chromium addition was 1810 μg/kg. The results of the sensitivity analysis showed low sensitivity and high stability of the meta-analysis.

Conclusions: CrPic supplementation had a positive effect on the growth performance of broilers, and this meta-analysis provides a more accurate value of chromium addition, which may be beneficial for the practice of the broiler industry.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249527PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023458PMC
April 2021

A combination of ssGSEA and mass cytometry identifies immune microenvironment in muscle-invasive bladder cancer.

J Clin Lab Anal 2021 May 4;35(5):e23754. Epub 2021 Apr 4.

Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, China.

Background: Muscle-invasive bladder cancer (MIBC) is a heterogeneous disease with varying clinical courses and responses to treatment. To improve the prognosis of patients, it is necessary to understand such heterogeneity.

Methods: We used single-sample gene set enrichment analysis to classify 35 MIBC cases into immunity-high and immunity-low groups. Bioinformatics analyses were conducted to compare the differences between these groups. Eventually, single-cell mass cytometry (CyTOF) was used to compare the characteristics of the immune microenvironment between the patients in the two groups.

Results: Compared with patients in the immunity-low group, patients in the immunity-high group had a higher number of tumor-infiltrating immune cells and greater enrichment of gene sets associated with antitumor immune activity. Furthermore, positive immune response-related pathways were more enriched in the immunity-high group. We identified 26 immune cell subsets, including cytotoxic T cells (Tcs), helper T cells (Ths), regulatory T cells (Tregs), B cells, macrophages, natural killer (NK) cells, and dendritic cells (DCs) using CyTOF. Furthermore, there was a higher proportion of CD45+ lymphocytes and enrichment of one Tc subset in the immunity-high group. Additionally, M2 macrophages were highly enriched in the immunity-low group. Finally, there was higher expression of PD-1 and Tim-3 on Tregs as well as a higher proportion of PD-1+ Tregs in the immunity-low group than in the immunity-high group.

Conclusion: In summary, the immune microenvironments of the immunity-high and immunity-low groups of patients with MIBC are heterogeneous. Specifically, immune suppression was observed in the immune microenvironment of the patients in the immunity-low group.
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http://dx.doi.org/10.1002/jcla.23754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128294PMC
May 2021

Effects of dietary inorganic chromium supplementation on broiler growth performance: a meta-analysis.

PeerJ 2021 16;9:e11097. Epub 2021 Mar 16.

Department of Life Sciences, Hulunbuir University, Hulunbuir, Inner Mongolia Autonomous Region, China.

Background: A meta-analysis was conducted to assess dietary inorganic chromium supplementation on broiler growth performance and determine if these effects are regulated by strains, sex, or contextual factors such as study area and time.

Methods: Eligible studies were identified by searching Web of Science, Springer, Elsevier, Science Direct, Taylor & Francis online databases. The weighted average difference with corresponding 95% confidence interval was computed with a random-effects model. We performed subgroup analyses stratified by study locations, published years, broiler sex, and strains. The publication bias was assessed with Egger's test method. A total of nine studies were eligible for inclusion.

Results: The meta-analysis results indicated that inorganic chromium supplementation significantly improved the broiler's growth performance, with a lower feed conversion ratio (FCR) and a higher average daily feed intake (ADFI). Through subgroup analyses, we found that the result of average daily gain (ADG) in Iran or published in the 2010s, the results of ADFI in Egypt, and the results of FCR in China had significant responses to chromium supplementation. We also found that Cobb 500 broilers and male broilers might be more sensitive to the addition of inorganic chromium by subgroup analyses. A model was used to obtain the amount of chromium addition under the optimal growth performance. The results showed that the adjusted ADFI and FCR presented a quadratic relationship with chromium supplementation except for average daily gain (ADG). The growth performance improved when the inorganic chromium addition ranged from 1.6 to 2.3 mg/kg. The result of sensitivity analyses showed low sensitivity and high stability. Also, there was little indication of publication bias for studies.

Conclusions: Our study showed that the males and Cobb 500 broilers might be more sensitive to chromium supplementation and provided more accurate inorganic chromium supplementation for broiler management practice. The fewer included studies may lead to higher heterogeneity, and no subgroup analyses of environmental stress conditions was conducted due to the lack of related information. Therefore, this study still has some limitations, and we look forward to the follow-up researches.
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http://dx.doi.org/10.7717/peerj.11097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977379PMC
March 2021

The noncoding RNAs SNORD50A and SNORD50B-mediated TRIM21-GMPS interaction promotes the growth of p53 wild-type breast cancers by degrading p53.

Cell Death Differ 2021 Mar 19. Epub 2021 Mar 19.

Key Laboratory for Tumor Precision Medicine of Shaanxi Province and Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, PR China.

Small nucleolar RNA SNORD50A and SNORD50B (SNORD50A/B) has been reported to be recurrently deleted and function as a putative tumor suppressor in different types of cancer by binding to and suppressing the activity of the KRAS oncoproteins. Its deletion correlates with poorer patient survival. However, in this study, we surprisingly found that SNORD50A/B loss predicted a better survival in breast cancer patients carrying wild-type p53. Functional studies showed that SNORD50A/B deletion strongly inhibited the proliferation, migration, invasion and tumorigenic potential, and induced cell cycle arrest and apoptosis in p53 wild-type breast cancer cells, while exerted the opposite effects in p53 mutated breast cancer cells. This was also supported by ectopically expressing SNORD50A/B in both p53 wild-type and mutated breast cancer cells. Mechanistically, SNORD50A/B clearly enhances the interaction between E3 ubiquitin ligase TRIM21 and its substrate GMPS by forming a complex among them, thereby promoting GMPS ubiquitination and its subsequent cytoplasmic sequestration. SNORD50A/B deletion in p53 wild-type breast cancer cells will release GMPS and induce the translocation of GMPS into the nucleus, where GMPS can recruit USP7 and form a complex with p53, thereby decreasing p53 ubiquitination, stabilizing p53 proteins, and inhibiting malignant phenotypes of cancer cells. Altogether, the present study first reports that SNORD50A/B plays an oncogenic role in p53 wild-type breast cancers by mediating TRIM21-GMPS interaction.
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http://dx.doi.org/10.1038/s41418-021-00762-7DOI Listing
March 2021

Self-Amplification of Coherent Energy Modulation in Seeded Free-Electron Lasers.

Phys Rev Lett 2021 Feb;126(8):084801

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.

The spectroscopic techniques for time-resolved fine analysis of matter require coherent x-ray radiation with femtosecond duration and high average brightness. Seeded free-electron lasers (FELs), which use the frequency up-conversion of an external seed laser to improve temporal coherence, are ideal for providing fully coherent soft x-ray pulses. However, it is difficult to operate seeded FELs at a high repetition rate due to the limitations of present state-of-the-art laser systems. Here, we report a novel self-modulation method for enhancing laser-induced energy modulation, thereby significantly reducing the requirement of an external laser system. Driven by this scheme, we experimentally realize high harmonic generation in a seeded FEL using an unprecedentedly small external laser-induced energy modulation. An electron beam with a laser-induced energy modulation as small as 1.8 times the slice energy spread is used for lasing at the seventh harmonic of a 266-nm seed laser in a single-stage high-gain harmonic generation (HGHG) setup and the 30th harmonic of the seed laser in a two-stage HGHG setup. The results mark a major step toward a high-repetition-rate, fully coherent x-ray FEL.
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http://dx.doi.org/10.1103/PhysRevLett.126.084801DOI Listing
February 2021

Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway.

Res Vet Sci 2021 May 3;136:220-226. Epub 2021 Mar 3.

College of Veterinary Medicine, Qingdao Agricultural University, Shandong, Qingdao 266109, PR China. Electronic address:

Endometritis is a major disease in productive bovines, and is also caused by conditional pathogens after delivery. The integrity and activity of bovine endometrial epithelial cells (bEECs) determine the development of endometritis. Tanshinone IIA, a compound purified from Salvia miltiorrhiza bunge, has been reported to have anti-inflammatory effects. The aim of this study was to investigate the anti-inflammatory effects of tanshinone IIA in the LPS-induced inflammatory response of bEECs. The results showed that tanshinone IIA inhibited the mRNA expression levels of COX-2 and iNOS, and reduced the expression levels of IL-1β, TNF-α, IL-6 and IL-8 induced by LPS. In addition, we found that tanshinone IIA inhibited the level of MDA, but increased the activities of CAT and SOD. To evaluate the anti-inflammatory mechanism of tanshinone IIA, we examined the activation of Nrf2. The results showed that the Nrf2 signaling pathway was significantly activated by tanshinone IIA. In conclusion, these results showed that tanshinone IIA exhibited anti-inflammatory and antioxidative effects by activating the Nrf2 signaling pathway.
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http://dx.doi.org/10.1016/j.rvsc.2021.03.004DOI Listing
May 2021

The Association between IL2 Genotypes and Risk and Severity of Chronic Periodontitis in a Chinese Han Population: A Case-control Study.

Immunol Invest 2021 Mar 8:1-7. Epub 2021 Mar 8.

Department of Stomatology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China.

Chronic periodontitis (CP) is a kind of multifactorial common oral diseases. Multiple immune molecules including interleukin-2 (IL-2) are involved in the occurrence and development of CP. To investigate the association between the IL2 rs2069762 polymorphism and the risk of CP in Chinese individuals, we recruited 375 CP patients and 443 controls in this case-control study. The PCR-RFLP method was used for genotyping. Data revealed that the GG genotype was related with a decreased risk of CP (GG vs TT: OR, 0.58, 95%CI, 0.37-0.92, -value = 0.020; GG vs TG+TT: OR, 0.61, 95%CI, 0.39-0.94, -value = 0.027). Besides, G allele was shown to decrease the risk of CP. In addition, the IL2 rs2069762 polymorphism was related with the severity of CP. To sum up, the IL2 rs2069762 polymorphism is related with a decreased risk and severity of CP in Chinese individuals.
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http://dx.doi.org/10.1080/08820139.2021.1885438DOI Listing
March 2021

Evaluation of the environmental costs of tourism based on an emergy analysis of tourism waste disposal: a case study of Yarlung Zangbo Grand Canyon National Park in Motuo County, Tibet.

Environ Sci Pollut Res Int 2021 Feb 25. Epub 2021 Feb 25.

Cultural Service Center of Dam Lhoba Ethnic Town, Motuo County, Lin Zhi, 860000, China.

Eco-tourism has become increasingly popular in the postmodern era. However, the management of tourism waste remains a major challenge for tourist destinations worldwide. Here, a non-participatory survey was conducted in five famous scenic spots in Yarlung Zangbo Grand Canyon National Park in Motuo County, Tibet, to characterize the waste composition and the amount of average daily production per capita during sightseeing. In addition, interviews were conducted at 26 restaurants and 32 hotels in Motuo Town (the administrative center of Motuo County), and data on the composition and amount of average daily production per capita of waste generated by tourists during accommodation and meals were obtained. The total amount of tourism waste in Motuo County in 2018 was approximately 172,108.82 kg. Based on the data collected, an emergy analysis was applied to emergy calculations of the pollution and losses generated during two conventional and locally used tourism waste disposal methods. According to China's emergy to money ratio (EMR) of 2018, the emergy was converted into its monetary value. The theoretical ecological compensation standard for Motuo County was 4,293,568.99 CNY (equivalent to 648,830.20 USD), and the average ticket price for a single tourist was 18.87 CNY (equivalent to 2.85 USD) in the absence of government fiscal transfer payments. These findings should be utilized by local national park authorities to establish a market-oriented ecological compensation mechanism that is capable of alleviating environmental pressure.
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http://dx.doi.org/10.1007/s11356-021-12829-6DOI Listing
February 2021

Novel Strategy for Mining and Identification of Acylcarnitines Using Data-Independent-Acquisition-Based Retention Time Prediction Modeling and Pseudo-Characteristic Fragmentation Ion Matching.

J Proteome Res 2021 03 24;20(3):1602-1611. Epub 2021 Feb 24.

School of Public Health/ MOE Key Lab for Public Health, Fudan University, Shanghai 200032, China.

It is a challenging work to screen, identify, and quantify acylcarnitines in complex biological samples. A method, based on the retention time (RT) prediction and data-independent acquisition strategies, was proposed for the large-scale identification of acylcarnitines using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). Relative cumulative eluotropic strength was introduced as a novel descriptor in building a linear prediction model, which not only solves the problem that acylcarnitines with long carbon chains cannot be well predicted in traditional models but also proves its robustness and transferability across instruments in two data sets that were acquired in distinct chromatography conditions. The accessibility of both predictive RT and MS spectra of suspect features effectively reduced about 30% false-positive results, and consequently, 150 and 186 acylcarnitines were identified in the rat liver and human plasma (NIST SRM 1950), respectively. This method provides a new approach in large-scale analysis of acylcarnitine in lipidomic studies and can also be extended to the analysis of other lipids.
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http://dx.doi.org/10.1021/acs.jproteome.0c00810DOI Listing
March 2021

Traditional Chinese Medicine for Patients of Coronary Artery Disease: Savior or Troublemaker? A Cohort Study About the Adherence to Antiplatelet Therapy.

J Gen Intern Med 2021 Feb 23. Epub 2021 Feb 23.

Department of Cardiology, the Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, China.

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http://dx.doi.org/10.1007/s11606-021-06640-9DOI Listing
February 2021

A Biaxial Strain Sensor Using a Single MoS Grating.

Nanoscale Res Lett 2021 Feb 10;16(1):31. Epub 2021 Feb 10.

Hefei National Research Center for Physical Sciences at the Microscale, Department of Materials Science and Engineering, CAS Key Lab of Materials for Energy Conversion, University of Science and Technology of China, Hefei, 230026, Anhui, China.

In this paper, we report a new type of MoS-based grating sensor for in-plane biaxial strain gauges with a precision limit of ~ 1‰. The MoS grating is numerically simulated with different biaxial strains up to 5%. Our first-principles calculations reveal that the strain sensitivity of the MoS reflectance spectrum can be considered an additional strain sensor integrated with the grating structure, enabling the mapping of in-plane biaxial strains. Our experimental studies on a prototype MoS-grating sensor further confirm that a strain component perpendicular to the grating period can cause intensity peak shifts in the grating's first-order diffraction patterns. This work opens a new path towards the sensing of in-plane biaxial strain within a single-grating device. Our new approach is applicable for other materials that have predictable reflectance response under biaxial strains and the capacity to form a two-dimensional single-crystal layer.
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http://dx.doi.org/10.1186/s11671-021-03493-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876191PMC
February 2021

HDAC inhibition potentiates anti-tumor activity of macrophages and enhances anti-PD-L1-mediated tumor suppression.

Oncogene 2021 Mar 9;40(10):1836-1850. Epub 2021 Feb 9.

The First Affiliated Hospital of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Institutes for Translational Medicine, Soochow University Medical College, Suzhou, 215123, Jiangsu, China.

Despite the widespread use of the blockade of immune checkpoints, for a significant number of cancer patients, these therapies have proven ineffective, presumably due to the immunosuppressive nature of the tumor microenvironment (TME). Critical drivers of immune escape in the TME include tumor-associated macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs), which not only mediate immune suppression, but also facilitate metastatic dissemination and impart resistance to immunotherapies. Thus, strategies that convert them into tumor fighters may offer great therapeutic potential. In this study, we evaluated whether pharmacologic modulation of macrophage phenotype by HDAC inhibitors (HDACi) could produce an anti-tumor effect. We demonstrated that low-dose HDACi trichostatin-A (TSA) markedly reshaped the tumor immune microenvironment by modulating the suppressive activity of infiltrating macrophages and inhibiting the recruitment of MDSCs in various tumors. These actions, in turn, augmented anti-tumor immune responses and further enhanced anti-tumor effects of immunotherapies. HDAC inhibition, however, also upregulated PD-L1, thereby limiting the beneficial therapeutic effects. Indeed, combining low-dose TSA with anti-PD-L1 in this model significantly enhanced the durability of tumor reduction and prolonged survival of tumor-bearing mice, compared with the effect of either treatment alone. These data introduce HDAC inhibition as a potential means to harness the anti-tumor potential of macrophages in cancer therapy.
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http://dx.doi.org/10.1038/s41388-020-01636-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946638PMC
March 2021

IL-36γ-armed oncolytic virus exerts superior efficacy through induction of potent adaptive antitumor immunity.

Cancer Immunol Immunother 2021 Feb 4. Epub 2021 Feb 4.

UPMC Hillman Cancer Center, Pittsburgh, PA, USA.

In this study, we aimed to apply the cytokine IL-36γ to cancer immunotherapy by constructing new oncolytic vaccinia viruses (OV) expressing interleukin-36γ (IL-36γ-OVs), leveraging unique synergism between OV and IL-36γ's ability to promote antitumor adaptive immunity and modulate tumor microenvironment (TME). IL-36γ-OV had dramatic therapeutic efficacies in multiple murine tumor models, frequently leading to complete cancer eradication in large fractions of mice. Mechanistically, IL-36-γ-armed OV induced infiltration of lymphocytes and dendritic cells, decreased myeloid-derived suppressor cells and M2-like tumor-associated macrophages, and T cell differentiation into effector cells. Further study showed that IL-36γ-OV increased the number of tumor antigen-specific CD4 and CD8 T cells and the therapeutic efficacy depended on both CD8 and CD4 T cells. These results demonstrate that these IL36γ-armed OVs exert potent therapeutic efficacy mainly though antitumor immunity and they may hold great potential to advance treatment in human cancer patients.
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http://dx.doi.org/10.1007/s00262-021-02860-4DOI Listing
February 2021

Susceptibility Factors of Stomach for SARS-CoV-2 and Treatment Implication of Mucosal Protective Agent in COVID-19.

Front Med (Lausanne) 2020 14;7:597967. Epub 2021 Jan 14.

The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

This work aims to study the gastrointestinal (GI) symptoms in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected patients and the susceptibility factors of the stomach for SARS-CoV-2. We investigated the SARS-CoV-2 susceptibility by analyzing the expression distribution of viral entry-associated genes, and , in single-cell RNA sequencing data derived from 12 gastric mucosa samples. We also analyzed the epidemiological, demographic, clinical, and laboratory data of 420 cases with SARS-CoV-2-caused coronavirus disease 2019 (COVID-19). and are specifically expressed in enterocytes which are mainly from gastric mucosa samples with () infection history and intestinal metaplasia (IM). A total of 420 patients were surveyed, of which 62 were with and 358 were without GI symptoms. There is a significant difference in average hospital stay ( < 0.001) and cost ( < 0.001) between the two groups. Among 23 hospitalized patients including seven with upper GI symptoms and 16 with lower GI symptoms, six (85.7%) and five (31.3%) had infection history, respectively ( = 0.03). Of 18 hospitalized patients with initial upper GI symptoms, none of the eight patients with mucosal protective agent therapy (e.g., sucralfate suspension gel, hydrotalcite tablets) had diarrhea subsequently, whereas six out of 10 patients without mucosal protective agent therapy had diarrhea subsequently ( = 0.01). IM and infection history may be susceptibility factors of SARS-CoV-2, and the mucosal protective agent may be useful for the blockade of SARS-CoV-2 transmission from the stomach to the intestine.
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http://dx.doi.org/10.3389/fmed.2020.597967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840564PMC
January 2021