Publications by authors named "Chao Dong"

236 Publications

Meta-analysis of the effects of 1-methylcyclopropene (1-MCP) treatment on climacteric fruit ripening.

Hortic Res 2020 Dec 3;7(1):208. Epub 2020 Dec 3.

College of Horticulture, Nanjing Agricultural University, Nanjing, China.

1-Methylcyclopropene (1-MCP) is an inhibitor of ethylene perception that is widely used to maintain the quality of several climacteric fruits during storage. A large body of literature now exists on the effects of 1-MCP on climacteric fruit ripening for different species and environmental conditions, presenting an opportunity to use meta-analysis to systematically dissect these effects. We classified 44 ripening indicators of climacteric fruits into five categories: physiology and biochemistry, quality, enzyme activity, color, and volatiles. Meta-analysis showed that 1-MCP treatment reduced 20 of the 44 indicators by a minimum of 22% and increased 6 indicators by at least 20%. These effects were associated with positive effects on delaying ripening and maintaining quality. Of the seven moderating variables, species, 1-MCP concentration, storage temperature and time had substantial impacts on the responses of fruit to 1-MCP treatment. Fruits from different species varied in their responses to 1-MCP, with the most pronounced responses observed in rosaceous fruits, especially apple, European pear fruits, and tropical fruits. The effect of gaseous 1-MCP was optimal at 1 μl/l, with a treatment time of 12-24 h, when the storage temperature was 0 °C for temperate fruits or 20 °C for tropical fruits, and when the shelf temperature was 20 °C, reflecting the majority of experimental approaches. These findings will help improve the efficacy of 1-MCP application during the storage of climacteric fruits, reduce fruit quality losses and increase commercial value.
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http://dx.doi.org/10.1038/s41438-020-00405-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713375PMC
December 2020

Polysaccharides can improve the survival of Lactiplantibacillus plantarum subjected to freeze-drying.

J Dairy Sci 2021 Mar 11;104(3):2606-2614. Epub 2020 Dec 11.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:

Freeze-drying is one of the most commonly used methods of bacteria preservation. During this process, cryoprotectants can greatly reduce cellular damage. Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects. Therefore, explorations of other types of cryoprotectants are needed. This study aimed to explore the possibility of the macromolecular cryoprotectants and combinations of cryoprotectants to maintain bacterial activity. We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant. Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively. These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects. We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity. Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
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http://dx.doi.org/10.3168/jds.2020-19110DOI Listing
March 2021

Interleukin 32: A novel player in perioperative neurocognitive disorders.

Med Hypotheses 2020 Nov 2;144:110158. Epub 2020 Aug 2.

Department of Anesthesiology, The Third Xiangya Hospital of Central South University, Tongzipo Road 138#, Changsha 410013, Hunan, China. Electronic address:

Perioperative neurocognitive disorders (PND) are highly prevalent after surgery, especially in aged patients. PND results in long-term morbidity and mortality with unclear pathophysiologic mechanisms. As a key hallmark of PND, surgery-induced neuroinflammation resulted from the invading of exogenous tracers into the cerebral parenchyma, causing hippocampal neuroinflammation and cognitive impairment. IL-32, with different isoforms, played a significant regulatory role in various inflammatory diseases. Its prevalence in peripheral circulating blood was closely associated with the central nervous system (CNS) diseases. Beyond that, specific subtype of IL-32 was reported to involve in the neuroinflammation regulation in cerebral ischemia impairment, multiple sclerosis, Alzheimer's Disease, and so on. Thus, we speculate that IL-32 may participate in the regulation of the surgery-induced neuroinflammation during the parthenogenesis of PND. The isoforms, spatio-temporal regulation of IL-32 may determine its pro- or anti-inflammation properties in parthenogenesis of PND. Therefore, IL-32 could be a putative therapeutic target for the prevention and reversal of PND in the future.
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http://dx.doi.org/10.1016/j.mehy.2020.110158DOI Listing
November 2020

Corrigendum to "Prophylactic Effects of Sulforaphane on Depression-Like Behavior and Dendritic Changes in Mice After Inflammation" Journal of Nutritional Biochemistry 39 (2017) 134-144.

J Nutr Biochem 2021 Feb 23;88:108550. Epub 2020 Nov 23.

Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba 260-8670, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.jnutbio.2020.108550DOI Listing
February 2021

The intervention of valproic acid on the tumorigenesis induced by an environmental carcinogen of PAHs.

Toxicol Res (Camb) 2020 Sep 31;9(5):609-621. Epub 2020 Aug 31.

Department of Occupational Health and Occupational Medicine, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Shandong, Jinan, 250012, China.

This study investigated whether valproic acid (VPA, a histone deacetylase inhibitor) can interfere with the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs). A typical representative compound of PAHs, 7,12-Dimethylbenz[a]anthracene (DMBA), was used to induce rat breast cancer. The results showed that therapeutic concentration of VPA (50 and 100 mg/kg) delayed the occurrence of tumors, reduced tumor formation rate and attenuated tumors growth, and have a protective effect on normal tissues. The macrophage-mediated inflammatory response was found to be associated with the observed effect of VPA. In addition, we screened and validated a possible gene, , which was involved in DMBA-induced breast cancer development and can be inhibited by VPA.
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http://dx.doi.org/10.1093/toxres/tfaa069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640934PMC
September 2020

Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling.

Toxicol Appl Pharmacol 2020 12 7;409:115333. Epub 2020 Nov 7.

Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Glucocorticoids have been widely used in multiple inflammatory and autoimmune diseases. However, long-term glucocorticoid therapy may result in osteoporosis. The present study aimed to evaluate the potential therapeutic effects and investigate the underlying mechanisms of Daphnetin (Daph) on glucocorticoid-induced osteoporosis (GIOP). In vivo, male Sprague Dawley rats were intramuscularly injected with dexamethasone (DEX) to induce GIOP and Daph was given intraperitoneally. Bone histological changes, mineral content, microstructure parameters and bone turnover markers were detected. Gut microbiota composition and intestinal barrier function were further assessed. In vitro, MC3T3-E1 pre-osteoblasts were treated with DEX and the abilities of Daph on osteoblast proliferation, differentiation and mineralization were assessed. A Wnt signaling inhibitor, XAV939, was added additionally to evaluate the effect of Daph on Wnt signaling. The results showed that in vivo, Daph increased the DEX-induced reduction in body weight gain, bone mineral content and microstructure parameters and restored the levels of bone turnover markers in GIOP rats. In vitro, Daph promoted osteoblast proliferation, differentiation and mineralization in DEX-treated MC3T3-E1 pre-osteoblasts. Moreover, Daph activated the Wnt/GSK-3β/β-catenin signaling pathway. XAV939 successfully abolished the beneficial effects of Daph on GIOP in vitro. Besides, Daph showed improvement on gut microbiota disorder and intestinal barrier dysfunction post GIOP. Collectively, these data demonstrated that Daph effectively ameliorates GIOP and the possible mechanism may be that Daph activated Wnt/GSK-3β/β-catenin signaling.
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http://dx.doi.org/10.1016/j.taap.2020.115333DOI Listing
December 2020

Assessment of the Understanding of Concussion and Care Protocols Amongst Student Athletes and Coaches: A Qualitative Study.

Front Pediatr 2020 24;8:526986. Epub 2020 Sep 24.

Duke-NUS Medical School, Singapore, Singapore.

Pediatric sports-induced concussions have become a topic of interest and concern in the scientific community. Already, the literature is rich with studies that have identified numerous short-term and long-term consequences of childhood sports-induced concussions. However, there are very few studies that have identified how well the students who participate in concussion-prone sports and their coaches understand these consequences and how they can be avoided. This study aimed to explore student athletes' and their coaches' understanding of the concept of concussion and how it is managed both immediately after the injury occurs and during long-term recovery. This study utilized a qualitative design. The study was conducted in local and international schools in Singapore. Participants were recruited through purposive sampling. 42 student athletes aged 13-18 who participated in rugby, softball, football, cricket, volleyball, and/or water polo were recruited. Fourteen coaches who coached these same sports were also recruited. Four focus groups and three semi-structured interviews were conducted. Data collected were then analyzed with thematic analysis. Risk factors were assessed through four domains of focus: understanding of what concussion is; attitudes toward concussion; existing protocols for treating concussion; and return-to-school and return-to-play protocols. As this is a qualitative study, outcome measures were not identified. Analysis of the data revealed four themes for each group. For student-athletes these included: limited understanding of concussion; non-reporting of injuries; variable supervision of athletes; and a lack of established return-to-school and return-to-play guidelines. For coaches these included: variable understanding of concussion; insufficient formal training in concussion management; limited medical support in managing injuries; and lack of understanding and adherence to return-to-school and return-to-play protocols. Of the themes identified, the most pressing was a lack of clearly defined return-to-play guidelines. This is an urgent issue that needs to be jointly addressed by healthcare professionals and schools with evidence-based guidelines.
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http://dx.doi.org/10.3389/fped.2020.526986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542181PMC
September 2020

Prediction of Radiosensitivity in Head and Neck Squamous Cell Carcinoma Based on Multiple Omics Data.

Front Genet 2020 18;11:960. Epub 2020 Aug 18.

Key Laboratory of Intelligent Computing and Signal Processing of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, China.

Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor. Radiotherapy (RT) is an important treatment for HNSCC, but not all patients derive survival benefit from RT due to the individual differences on radiosensitivity. A prediction model of radiosensitivity based on multiple omics data might solve this problem. Compared with single omics data, multiple omics data can illuminate more systematical associations between complex molecular characteristics and cancer phenotypes. In this study, we obtained 122 differential expression genes by analyzing the gene expression data of HNSCC patients with RT ( = 287) and without RT ( = 189) downloaded from The Cancer Genome Atlas. Then, HNSCC patients with RT were randomly divided into a training set ( = 149) and a test set ( = 138). Finally, we combined multiple omics data of 122 differential genes with clinical outcomes on the training set to establish a 12-gene signature by two-stage regularization and multivariable Cox regression models. Using the median score of the 12-gene signature on the training set as the cutoff value, the patients were divided into the high- and low-score groups. The analysis revealed that patients in the low-score group had higher radiosensitivity and would benefit from RT. Furthermore, we developed a nomogram to predict the overall survival of HNSCC patients with RT. We compared the prognostic value of 12-gene signature with those of the gene signatures based on single omics data. It suggested that the 12-gene signature based on multiple omics data achieved the best ability for predicting radiosensitivity. In conclusion, the proposed 12-gene signature is a promising biomarker for estimating the RT options in HNSCC patients.
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http://dx.doi.org/10.3389/fgene.2020.00960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461877PMC
August 2020

Effect of Early Normobaric Hyperoxia on Blast-Induced Traumatic Brain Injury in Rats.

Neurochem Res 2020 Nov 9;45(11):2723-2731. Epub 2020 Sep 9.

Department of Neurosurgery, Sixth Medical Center, Chinese PLA General Hospital, No. 6, Fucheng Road, Haidian District, Beijing, 100048, China.

Blast-induced traumatic brain injury (bTBI) is a leading cause of disability and mortality in soldiers during the conflicts in Iraq and Afghanistan. Although substantial clinical and animal studies have investigated the pathophysiology and treatments of bTBI, few effective therapies have been found, especially for the early rescue in the battlefield. The aim of this study is to evaluate neuroprotective effects of early normobaric hyperoxia (NBO) on bTBI. We established a rat model of bTBI caused by explosion in the cabin. It exhibited typical changes of mild bTBI, like impaired neurological function, brain edema, minor intracranial hemorrhage and neuron necrosis. The rats were divided into 4 groups (n = 12): Sham, Vehicle, hyperbaric oxygen (HBO) and NBO. Neurological function of the rats was assessed by the Neurological Severity Scores (NSS) at 24 h and 72 h after explosion. Serum interleukin-6 (IL-6), neuron specific enolase (NSE) and tau protein were measured at 24 h and 72 h after explosion. Brain water content was measured and Aquaporin-4 (AQP4) immunostaining was performed. Neuronal apoptosis was analyzed by TUNEL staining. NBO demonstrated curative effects on protecting the neurological function. Serum levels of NSE and tau protein were reduced at 24 h and 72 h after explosion. But the levels of IL-6 were not reduced significantly at both time points. Cerebral edema was alleviated. Simultaneously, AQP4 immunostaining of the hippocampus showed remarkably decreased expression after treatment. The number of apoptotic cells in hippocampus was also decreased. Compared with HBO, NBO is simple and convenient, and can be administered in remote areas. It may be a promising therapy for early rescue of bTBI in the battlefield.
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http://dx.doi.org/10.1007/s11064-020-03123-xDOI Listing
November 2020

Discovery of 4-methyl-N-(4-((4-methylpiperazin- 1-yl)methyl)-3-(trifluoromethyl)phenyl)-3-((6-(pyridin-3-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-yl)-oxy)benzamide as a potent inhibitor of RET and its gatekeeper mutant.

Eur J Med Chem 2020 Dec 22;207:112755. Epub 2020 Aug 22.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China; State-province Joint Engineering Laboratory of Targeted Drugs from Natural Products, Xiamen University, Xiamen, Fujian, 361102, China. Electronic address:

The receptor tyrosine kinase rearranged during transfection (RET) plays pivotal roles in several cancers, including thyroid carcinoma and non-small cell lung cancer (NSCLC). Currently, there are several FDA-approved RET inhibitors, but their indication is limited to thyroid cancer, and none can overcome their gatekeeper mutants (V804L and V804M). Here, we report the discovery of 9x representing a new chemotype of potent and selective RET inhibitors, using a rational design strategy of type II kinase inhibitors. 9x exhibited both superior antiproliferative activities against NSCLC-related carcinogenic fusions KIF5B-RET and CCDC6-RET and gatekeeper mutant-transformed Ba/F3 cells, with the lowest GI of 9 nM, and substantial inhibitory activities against wild-type RET and RET mutant proteins, with the best IC of 4 nM. More importantly, 9x also showed nanomole potency against RET-positive NSCLC cells LC-2/ad, but not against a panel of RET-negative cancer cells, such as A549, H3122, A375 or parental Ba/F3 cells, demonstrating its selective 'on-target' effect. In mouse xenograft models, 9x repressed tumor growth driven by both wild type KIF5B-RET-Ba/F3 and gatekeeper mutant KIF5B-RET(V804M)-Ba/F3 cells in a dose-dependent manner. Together, these data establish that 9x provides a good starting point for the development of targeted therapeutics against RET-positive cancers, especially NSCLC.
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http://dx.doi.org/10.1016/j.ejmech.2020.112755DOI Listing
December 2020

The sugar transporter system of strawberry: genome-wide identification and expression correlation with fruit soluble sugar-related traits in a  ×  germplasm collection.

Hortic Res 2020 27;7:132. Epub 2020 Jul 27.

Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, 201403 China.

Sugar from plant photosynthesis is a basic requirement for life activities. Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs. The transporters of the major facilitator superfamily (MFS) targeting carbohydrates represent the largest family of sugar transporters in many plants. Strawberry ( ×  Duchesne) is an important crop appreciated worldwide for its unique fruit flavor. The involvement of MFS sugar transporters (STs) in cultivated strawberry fruit sugar accumulation is largely unknown. In this work, we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 varieties. Then, a total of 67 genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of , the dominant subgenome provider for modern cultivated strawberry. Phylogenetic analysis updated the nomenclature of strawberry ST homoeologs. Both the chromosomal distribution and structural characteristics of the ST family were improved. Semi-RT-PCR analysis in nine tissues from cv. Benihoppe screened 34 highly expressed genes in fruits. In three varieties with dramatically differing fruit sugar levels, qPCR integrated with correlation analysis between transcript abundance and sugar content identified 13 sugar-correlated genes. The correlations were re-evaluated across 19 varieties, including major commercial cultivars grown in China. Finally, a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was proposed. Our work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.
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http://dx.doi.org/10.1038/s41438-020-00359-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385174PMC
July 2020

Dissipation and residue determination of penicillin G and its two metabolites in citrus under field conditions by DSPE/UPLC-MS/MS.

Biomed Chromatogr 2020 Dec 26;34(12):e4962. Epub 2020 Aug 26.

Citrus Research Institute, Southwest University & Chinese Academy of Agricultural Sciences, Chongqing, China.

A rapid determination method of residual penicillin G and its two metabolites in citrus was developed and validated by dispersive solid-phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (DSPE/UPLC-MS/MS). The samples were extracted with 80% acetonitrile and purified with octadecylsilane. High linearity was obtained with correlation coefficients (r ) >0.9981. The limits of quantification were 0.005-0.01 mg/kg. The recoveries of penicillin G and its metabolites spiked in blank citrus were within 76.7-107%, with relative standard deviations of 1.3-9.6%. The dissipation dynamics and distribution of penicillin G in citrus followed first-order kinetics, with half-life of 1.7-2.7 days. Penicillin G degraded easily in citrus and the metabolite was mainly penilloic acid, which can exist stably for long time. The terminal residues of penicillin G in pulp, whole citrus and peels were 0.015-0.701, 0.047-7.653 and 0.162-13.376 mg/kg, respectively. The hazard indexes for risk assessment of citrus were significantly <1, suggesting that the health risks to humans after consumption of citrus were insignificant and negligible. These results could provide necessary data for evaluating the safe and proper use of penicillin G in citrus.
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http://dx.doi.org/10.1002/bmc.4962DOI Listing
December 2020

CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma.

J Exp Clin Cancer Res 2020 Jul 29;39(1):145. Epub 2020 Jul 29.

National Translational Science Center for Molecular Medicine, Department of Cell Biology, Fourth Military Medical University, Xi'an, 710032, P. R. China.

Background: Mounting evidence suggests that solid tumors display the features of collective invasion, however, the molecular mechanisms are far from clear. This study aims to verify the role and the underlying mechanisms of CD147 in collective invasion in hepatocellular carcinoma.

Methods: Immunostaining was used to analyze human hepatocellular carcinoma specimens and three-dimensional cultures. Three-dimensional invasion model was established to mimic in vivo invasion. RNA-sequencing was used to identify downstream effectors.

Results: Human hepatocellular carcinoma underwent collective invasion and CD147 was observed to be upregulated at the invasive front of tumor cell groups. CD147 was demonstrated to promote collective invasion using the modified three-dimensional invasion model, which recapitulated the main features of collective invasion. Through transcriptome analysis and enzyme activity assay, we found that CD147 enhanced cathepsin B expression and activity. Upregulated cathepsin B in hepatocellular carcinoma cells facilitated migration and invasion, which mediated CD147-induced invasive phenotype in hepatocellular carcinoma. In terms of mechanism, we found that CD147 promoted cathepsin B transcription by activating β-catenin signaling as a result of reduced GSK-3β expression. Furthermore, we found that elevated expression of CD147 as well as cathepsin B were correlated with poor prognosis in patients with hepatocellular carcinoma.

Conclusions: CD147 promotes hepatocellular carcinoma cells collective invasion via upregulating cathepsin B expression and targeting CD147 would be valuable for the development of novel therapeutic modalities against invasion and metastasis of cancer.
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http://dx.doi.org/10.1186/s13046-020-01647-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391525PMC
July 2020

Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells.

Development 2020 07 3;147(13). Epub 2020 Jul 3.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

In mammalian growing follicles, oocytes are arrested at the diplotene stage (which resembles the G2/M boundary in mitosis), while the granulosa cells (GCs) continue to proliferate during follicular development, reflecting a cell cycle asynchrony between oocytes and GCs. Hypoxanthine (Hx), a purine present in the follicular fluid, has been shown to induce oocytes meiotic arrest, although its role in GC proliferation remains ill-defined. Here, we demonstrate that Hx indiscriminately prevents G2-to-M phase transition in porcine GCs. However, oocyte-derived paracrine factors (ODPFs), particularly GDF9 and BMP15, maintain the proliferation of GCs, partly by activating the ERK1/2 signaling and enabling the G2/M transition that is suppressed by Hx. Interestingly, GCs with lower expression of GDF9/BMP15 receptors appear to be more sensitive to Hx-induced G2/M arrest and become easily detached from the follicular wall. Importantly, Hx-mediated inhibition of G2/M progression instigates GC apoptosis, which is ameliorated in the presence of GDF9 and/or BMP15. Therefore, our data indicate that the counterbalance of intrafollicular factors, particularly Hx and oocyte-derived GDF9/BMP15, fine-tunes the development of porcine follicles by regulating the cell cycle progression of GCs.
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http://dx.doi.org/10.1242/dev.184838DOI Listing
July 2020

Screen identifies DYRK1B network as mediator of transcription repression on damaged chromatin.

Proc Natl Acad Sci U S A 2020 07 1;117(29):17019-17030. Epub 2020 Jul 1.

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;

DNA double-strand breaks (DSBs) trigger transient pausing of nearby transcription, an emerging ATM-dependent response that suppresses chromosomal instability. We screened a chemical library designed to target the human kinome for new activities that mediate gene silencing on DSB-flanking chromatin, and have uncovered the DYRK1B kinase as an early respondent to DNA damage. We showed that DYRK1B is swiftly and transiently recruited to laser-microirradiated sites, and that genetic inactivation of DYRK1B or its kinase activity attenuated DSB-induced gene silencing and led to compromised DNA repair. Notably, global transcription shutdown alleviated DNA repair defects associated with DYRK1B loss, suggesting that DYRK1B is strictly required for DSB repair on active chromatin. We also found that DYRK1B mediates transcription silencing in part via phosphorylating and enforcing DSB accumulation of the histone methyltransferase EHMT2. Together, our findings unveil the DYRK1B signaling network as a key branch of mammalian DNA damage response circuitries, and establish the DYRK1B-EHMT2 axis as an effector that coordinates DSB repair on transcribed chromatin.
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http://dx.doi.org/10.1073/pnas.2002193117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382216PMC
July 2020

WITHDRAWN: The Role of Intercostal Nerve Block Combined with Puncture Surgery Robot Controlled by Fuzzy Proportion Integral Differential Algorithm under the Guidance of MRI Image in the Treatment of Lung Cancer.

Neurosci Lett 2020 Jun 23:135204. Epub 2020 Jun 23.

Department of Thoracic Surgery, The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou City, 730050, Gansu Province, China.

This article has been withdrawn at the request of the Editor-in-Chief. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
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http://dx.doi.org/10.1016/j.neulet.2020.135204DOI Listing
June 2020

Hollow Carbon Sphere Nanoreactors Loaded with PdCu Nanoparticles: Void-Confinement Effects in Liquid-Phase Hydrogenations.

Angew Chem Int Ed Engl 2020 Oct 18;59(42):18374-18379. Epub 2020 Aug 18.

Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.

Nanoreactors with hollow structures have attracted great interest in catalysis research due to their void-confinement effects. However, the challenge in unambiguously unraveling these confinement effects is to decouple them from other factors affecting catalysis. Here, we synthesize a pair of hollow carbon sphere (HCS) nanoreactors with presynthesized PdCu nanoparticles encapsulated inside of HCS ([email protected]) and supported outside of HCS (PdCu/HCS), respectively, while keeping other structural features the same. Based on the two comparative nanoreactors, void-confinement effects in liquid-phase hydrogenation are investigated in a two-chamber reactor. It is found that hydrogenations over [email protected] are shape-selective catalysis, can be accelerated (accumulation of reactants), decelerated (mass transfer limitation), and even inhibited (molecular-sieving effect); conversion of the intermediate in the void space can be further promoted. Using this principle, a specific imine is selectively produced. This work provides a proof of concept for fundamental catalytic action of the hollow nanoreactors.
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http://dx.doi.org/10.1002/anie.202007297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590117PMC
October 2020

Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway.

Aging (Albany NY) 2020 06 22;12(12):12002-12018. Epub 2020 Jun 22.

Department of Dermatology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, P.R. China.

Extracellular vesicles are involved in skin wound healing and diabetes. After enrichment and identification, plasma endothelial cells-derived-extracellular vesicles were cocultured with skin fibroblasts or HaCaT. The gain-and loss-of functions were performed to measure fibroblast proliferation, senescence, and reactive oxygen species. Levels of senescence-related proteins, senescence-associated secretory phenotypes, vascular markers, YAP and the PI3K/Akt/mTOR pathway-related proteins were determined. Diabetic mice were induced to establish skin wound model. After endothelial cells-derived-extracellular vesicles were injected into skin wound modeling mice, skin wound healing was evaluated. Endothelial cells-derived-extracellular vesicles treatment enhanced fibroblast proliferation, and decreased senescence through the elevation of YAP nuclear translocation and activation the PI3K/Akt/mTOR pathway. YAP inhibition reversed the effect of plasma endothelial cells-derived-extracellular vesicles on fibroblast proliferation. Endothelial cells-derived-extracellular vesicles also promoted wound healing in diabetic mice, increased microvascular density, collagen deposition, macrophage infiltration and positive rates of vascular markers, and inhibited YAP phosphorylation and senescence. Plasma endothelial cells-derived-extracellular vesicles prevent fibroblast senescence and accelerate skin wound healing in diabetic mice by reducing YAP phosphorylation and activating the PI3K/Akt/mTOR pathway. This study may provide novel insights for skin disorders in diabetic mice.
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http://dx.doi.org/10.18632/aging.103366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343472PMC
June 2020

Anagliptin stimulates osteoblastic cell differentiation and mineralization.

Biomed Pharmacother 2020 Sep 16;129:109796. Epub 2020 Jun 16.

Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Osteoporosis is a common debilitating bone disease characterized by loss of bone mass and degradation of the bone architecture, which is primarily driven by dysregulated differentiation of mesenchymal stem cells into bone-producing osteoblasts. Osteoblasts contribute to bone formation by secreting various proteins that guide the deposition of bone extracellular matrix, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). The Wnt/β-catenin pathway is widely recognized as a regulator of bone mass and is required to maintain bone homeostasis. Hormones have long been recognized as playing a key role in bone metabolism, and in recent years, growing evidence has shown that diabetes is a risk factor for osteoporosis. In the present study, we investigated the effects of the antidiabetic drug anagliptin on the differentiation and mineralization of osteoblasts induced by osteogenic medium. Anagliptin promotes insulin production via inhibition of dipeptidyl peptidase IV (DPP-4), an enzyme that targets the incretin hormone glucagon-like peptide 1 (GLP-1) for degradation. Our findings show that anagliptin significantly increases the differentiation of MSCs into osteoblasts via activation of RUNX2. Anagliptin significantly increased matrix deposition and mineralization by osteoblasts, as evidenced by elevated levels of ALP, OCN, OPN, and BMP-2. We further demonstrate that anagliptin activates the canonical and noncannonical Wnt signaling pathways and that silencing of Wnt/β-catenin signaling completely abolished the effects of anagliptin. Thus, anagliptin might be a safe, effective therapy for type II diabetes that might show promise as a therapy against osteoporosis.
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http://dx.doi.org/10.1016/j.biopha.2019.109796DOI Listing
September 2020

Magnetic and magneto-transport studies of two-dimensional ferromagnetic compound FeGeTe.

J Phys Condens Matter 2020 Jun 11;32(40):405805. Epub 2020 Jun 11.

Wuhan National High Magnetic Field Center and School of Physics, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China.

We have systematically reported the magnetic and magneto-transport properties of two-dimensional itinerant ferromagnetic compound FeGeTe at high magnetic fields of 58 T and demonstrated the correlation between its transport and magnetism. Anomalous two-steps magnetic ordering and antiferromagnetic-like transitions in zero field-cooling (ZFC) curves for H ∥ ab-plane are observed. Additionally, we find that intrinsic negative magnetoresistances in bulk FeGeTe single crystal are mainly attributed to the suppression of spin-fluctuations in low magnetic fields. Complex evolutions of temperature dependent high field magnetoresistances are detected under different magnetic field and current configurations, which can be explained as a result of the competition between spin-fluctuations, the magnon-scatterings and classical cyclotronic effects.
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http://dx.doi.org/10.1088/1361-648X/ab9bc9DOI Listing
June 2020

Controlling factors for the distribution of typical organic pollutants in the surface sediment of a macrotidal bay.

Environ Sci Pollut Res Int 2020 Aug 15;27(22):28276-28287. Epub 2020 May 15.

Qingdao Institute of Marine Geology, China Geological Survey, Qingdao, 266071, China.

Marine sediment is the final sink of polycyclic aromatic hydrocarbons (PAHs) from river input and atmospheric deposition. Such kind of pollutant cycles in the marine environment is usually controlled by hydrodynamic conditions. Many previous studies have explored how ocean currents influence pollutant distribution, but very few studies have focused on the relationships between the distribution patterns of pollutant and residual currents or sediment transport. In this study, 16 monomers of PAHs from 135 surface sediment samples collected in the Hangzhou Bay, a typical macrotidal bay, were systematically identified and their sources were analyzed. The sediment characteristics and distribution pattern were also comprehensively analyzed. The results showed the seabed sediments were moderately polluted by PAHs with a level of 38.58-1371.06 ng/g (median 186.70 ng/g). Most of the PAHs are composed of three to five rings, originated from combustion of coal and firewood. The combustion of oil also contributes to PAHs in seabed sediments for some areas. The PAHs are found to be concentrated within the estuary and the offshore areas, as well in coastal ocean. Fine-grained sediment transport is controlled by residual currents, which leads to PAH accumulation in the bay and the offshore areas, forming a high-value distribution pattern. Hence, we conclude that residual current is the main factors that control the long-term distribution of PAHs in the seabed sediments of the macrotidal bay. Temporal and spatial varying models of PAHs were needed in a further study to explore further the mechanisms how PAHs are transported in coastal areas.
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http://dx.doi.org/10.1007/s11356-020-09199-wDOI Listing
August 2020

LncRNA RPSAP52 Upregulates TGF-β1 to Increase Cancer Cell Stemness and Predict Postoperative Survival in Glioblastoma.

Cancer Manag Res 2020 15;12:2541-2547. Epub 2020 Apr 15.

Department of Neurosurgery, Sixth Medical Center, Chinese PLA General Hospital, Beijing 100048, People's Republic of China.

Introduction: Ribosomal protein SA pseudogene 52 (RPSAP52) has been characterized as an oncogenic lncRNA in pituitary tumors. Analysis of TCGA dataset revealed the upregulation of RPSAP52 in glioblastoma (GBM). We, therefore, investigated the roles of RPSAP52 in GBM.

Methods: A total of 50 GBM patients (33 males and 20 females; 54-75 years; mean age: 61.8±5.8 years) were selected from the 89 cases of GBM patients. Under the guidance of MRI, brain biopsy was performed to collect GBM tissues from each patient for the diagnosis of GBM. U-373 MG cells were employed and had transient transfections. qRNA, Western blot, and a series of experiments were performed to characterize their associations.

Results: The results showed that RPSAP52 was upregulated in GBM patients, and its high expression levels predicted poor survival. In GBM tissues, expression levels of RPSAP52 were significantly and positively correlated with that of TGF-β1. In GBM tissues, RPSAP52 positively regulated the expression of TGF-β1. Cell stemness assay showed that, compared to C and NC groups, overexpression of RPSAP52 and TGF-β1 led to increased, while silencing of RPSAP52 led to decreased CD133+ cells. Overexpression of TGF-β1 attenuated the effects of RPSAP52 siRNA silencing.

Conclusion: Therefore, RPSAP52 upregulates TGF-β1 to increase cancer cell stemness and predict postoperative survival in GBM.
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http://dx.doi.org/10.2147/CMAR.S227496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170709PMC
April 2020

Simultaneous Enantioselective Determination of Two New Isopropanol-Triazole Fungicides in Plant-Origin Foods Using Multiwalled Carbon Nanotubes in Reversed-Dispersive Solid-Phase Extraction and Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.

J Agric Food Chem 2020 May 12;68(21):5969-5979. Epub 2020 May 12.

Citrus Research Institute, Southwest University & Chinese Academy of Agricultural Sciences, Chongqing 400712, People's Republic of China.

A simple and sensitive enantiomeric analytical method was established for the determination of two new isopropanol-triazole fungicides mefentrifluconazole and ipfentrifluconazole in plant-origin foods using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The best enantioseparation of the four target stereoisomers was achieved on a Chiral MX(2)-RH column within 7 min by reversed-phase liquid chromatography, which is a significant improvement in the resolution of different chiral compounds under one set of conditions. A simple and effective pretreatment procedure was developed for the extraction and purification of the two target chiral fungicides using reversed-dispersive solid-phase extraction (r-DSPE) with multiwalled carbon nanotubes (MWCNTs). The influence of the type and amount of MWCNTs on the purification efficiencies and recoveries was evaluated. The mean recoveries for all four stereoisomers were in the range of 76.9-91.2%, with relative standard deviation (RSD) values below 7.2%. The limit of quantification (LOQ) of all stereoisomers of mefentrifluconazole and ipfentrifluconazole was 5 μg/kg for all tested matrixes. The results of the method validation and real samples analysis confirm that the established method is efficient and reliable for the enantiomeric determination of mefentrifluconazole and ipfentrifluconazole in plant-origin food samples.
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http://dx.doi.org/10.1021/acs.jafc.0c01385DOI Listing
May 2020

Differential longitudinal changes in structural complexity and volumetric measures in community-dwelling older individuals.

Neurobiol Aging 2020 07 5;91:26-35. Epub 2020 Mar 5.

Centre for Healthy Brain Ageing, School of Psychiatry, University of New South Wales, Sydney, NSW, Australia; Neuropsychiatric Institute, Prince of Wales Hospital, Sydney, NSW, Australia.

Fractal geometry provides a method of analyzing natural and especially biological morphologies. To investigate the relationship between the complexity measure, which is indexed as fractal dimensionality (FD), and the traditional Euclidean metrics, such as the volume and thickness, of the brain in older age, we analyzed 483 MRI scans of 161 community-dwelling, nondemented individuals aged 70-90 years at the baseline and their 2-year and 6-year follow-ups. We quantified changes in neuroimaging metrics in cortical lobes and subcortical structures and investigated the effects of age, sex, hemisphere, and education on FD. We also analyzed the mediating effects of these metrics for further investigation. FD showed significant age-related decline in all structures, and its trajectory was best modeled quadratically in the bilateral frontal, parietal, and occipital lobes, as well as in the bilateral caudate, putamen, hippocampus, amygdala, and accumbens. FD showed specific mediating effects on aging and cognitive decline in some regions. Our findings suggest that FD is reliable yet shows a different pattern of decline compared with volumetric measures.
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http://dx.doi.org/10.1016/j.neurobiolaging.2020.02.023DOI Listing
July 2020

Effects of asymptomatic infection on the dynamical interplay between behavior and disease transmission in multiplex networks.

Physica A 2019 Dec 2;536:121030. Epub 2019 May 2.

School of Management and Economics, Beijing Institute of Technology, Beijing 100045, China.

Multiplex network theory is widely introduced to deepen the understanding of the dynamical interplay between self-protective behavior and epidemic spreading. Most of the existing studies assumed that all infected individuals can transmit disease- related information or can be perceived by their neighbors. However, owing to lack of distinct symptoms for patients in the initial stage of infection, the disease information cannot be transmitted in the population, which may lead to the wrong perception of infection risk and inappropriate behavior response. In this work, we divide infected individuals into Exposed-state (without obvious clinical symptoms) individuals and Infected-state (with evident clinical symptoms) individuals, both of whom can spread disease, but only Infected-state individuals can diffuse disease information. Then, in this work we establish UAU-SEIS (Unaware-Aware-Unaware-Susceptible-Exposed-Infected-Susceptible) model in multiplex networks and analyze the effect of asymptomatic infection and the isolation of Infected-state individuals on the density of infection and the epidemic threshold. Furthermore, we extend the UAU-SEIS model by taking the individual heterogeneity into consideration. Combined Markov chain approach and Monte-Carlo Simulations, we find that asymptomatic infection has an effect on the density of infected individuals and the epidemic threshold, and the extent of the effect is influenced by whether Infected-state individuals are isolated or treated. In addition, results show that the individual heterogeneity can lower the density of infected individuals, but cannot enhance the epidemic threshold.
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http://dx.doi.org/10.1016/j.physa.2019.04.266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125818PMC
December 2019

Longitudinal Changes in Whole-Brain Functional Connectivity Strength Patterns and the Relationship With the Global Cognitive Decline in Older Adults.

Front Aging Neurosci 2020 17;12:71. Epub 2020 Mar 17.

Centre for Healthy Brain Aging, School of Psychiatry, University of New South Wales, Sydney, NSW, Australia.

Aging is associated with changes in brain functional patterns as well as cognition. The present research sought to investigate longitudinal changes in whole brain functional connectivity strength (FCS) and cognitive performance scores in very old cognitively unimpaired individuals. We studied 34 cognitively normal elderly individuals at both baseline and 4-year follow-up (baseline age = 78 ± 3.14 years) with resting-state functional magnetic resonance imaging (r-fMRI), structural MRI scans, and neuropsychological assessments conducted. Voxel-based whole brain FCS was calculated and we found that bilateral superior parietal and medial frontal regions showed decreased FCS, while the supplementary motor area (SMA) and insula showed increased FCS with age, along with a decrease in bilateral prefrontal cortical thickness. The changes of FCS in left precuneus were associated with an aging-related decline in global cognition. Taken together, our results suggest changes in FCS with aging with the precuneus as a hub and this may underlie changes in global cognition that accompany aging. These findings help better understand the normal aging mechanism.
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http://dx.doi.org/10.3389/fnagi.2020.00071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090100PMC
March 2020

Percutaneous cyst aspiration with injection of two different bioresorbable bone cements in treatment of simple bone cyst.

J Child Orthop 2020 Feb;14(1):76-84

Department of Pediatric Orthopedics, University Children's Hospital Basel, Basel, Switzerland.

Introduction: Simple bone cysts (SBCs) are common in children and adolescents. The risk of refracture and the probability of spontaneous healing in SBCs are mainly dependent on the activity of the cyst and can be quantified with the Cyst-Index. Avoiding pathological fractures is the primary goal. Our study presents a comparison of two different bioresorbable bone graft substitutes (BGSs) in the minimally-invasive treatment of SBC in the active stage by percutaneous cyst aspiration and injection.

Methods: Between 2006 and 2017, 38 patients (aged two to 37 years; mean age 12.4 (sd 5.6)) were treated with percutaneous cyst aspiration and refilled with bioresorbable BGSs in three hospitals. The cysts of 21 patients (11 humerus, five femur, four calcaneus, one fibula) were refilled with porous beta-tricalcium phosphate (PB-TP group) (ChronOS Inject) and of 17 patients (nine humerus, six femur, one calcaneus, one fibula) with hydroxyapatite/calcium sulphate (H/CS group) (CERAMENT|BONE VOID FILLER). There were 13 (62%) preceding fractures in the PB-TP group and eight (47%) in the H/CS group. The follow-up of all patients was at least two years. During follow-up, radiological healing (modified Neer classification), activity level, refracture rates, recurrence rates, resorption period and complications were analyzed.

Results: In all, 21 patients treated with PB-TP group experienced 27 operations (one refracture, three recurrences and one persistent cyst). A total of 17 patients treated with H/CS experienced 20 operations (one refracture and one recurrence). After six weeks, 95% in the PB-TP group returned to unrestricted activity with one refracture in the femur due to insufficient biomechanical stability and all returned to unrestricted activity in the H/CS group. Partial or complete radiological response was observed in 81% after 13 months (sd 3.4). Three recurrences (14%) occurred in the PB-TP group and one recurrence (6%) occurred in the H/CS group. The refracture rates were similar in both groups; one (5%) in the PB-TP group and one (6%) in the H/CS group. All H/CS treated cysts showed completed resorption after two years, whilst in PB-TP treated cysts no resorption occurred in five cases (25%) (p = 0.031). Two (10%) wound infections occurred in the PB-TP group and no infections occurred in the H/CS group.

Conclusion: Both PB-TP and H/CS can provide stability and prevent refracture in patients with single bone cysts at the upper extremity or the foot. For the proximal femur, additional stabilization is necessary, due to the weight-bearing and associated high refracture rate. The H/CS bone graft substitute has a better resorption rate than the PB-TP graft.

Level Of Evidence: III.
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http://dx.doi.org/10.1302/1863-2548.14.190155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7043122PMC
February 2020

Nanocomplexes loaded with miR-128-3p for enhancing chemotherapy effect of colorectal cancer through dual-targeting silence the activity of PI3K/AKT and MEK/ERK pathway.

Drug Deliv 2020 Dec;27(1):323-333

Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.

Although microRNAs (miRNAs)-based cancer therapy strategies have been proved to be efficient and superior to chemotherapeutic agents in certain extent, the unstable properties of miRNAs significantly impaired the wide application. Therefore, how to safely deliver the miRNAs to the targeted site of action is the most pivotal step to achieve the ideal treatment effect. In the present work, the miR-128-3p, which is able of inducing chromosomal instability, was loaded into the nanocomplexes developed by the PEG-PDMAEMA (PDMAEMA-NP). By this way, the miR-128-3p was shielded from exposure to various degrading enzymes in bloodstream. Additionally, the PEGylation endowed the PDMAEMA-NP with long time of circulation as demonstrated by pharmacokinetics investigation. To target and deliver the miR-128-3p to the site of action, a tumor-homing peptide CPKSNNGVC, which specifically targets the monocarboxylate transporter 1 (MCT1), was decorated on the surface of PDMAEMA-NP. Both and experiments demonstrated that more efficient delivery of miR-128-3p to cells or tumor tissues was obtained by the PDMAEMA-NP than plasmid. Additionally, modification of C peptides further enhanced the tumor accumulation of miR-128-3p, and in turn contributed to the stronger tumor growth inhibition effect. Underlying mechanisms study revealed that the miR-128-3p inhibited the growth, migration, and invasion of colorectal cancer (CRC) cells and progress of CRC tissues through silence of the activity of PI3K/AKT and MEK/ERK pathway. By this way, the chemotherapy effect of 5-Fluorouracil (5-Fu) was dramatically improved after co-treating the cells with miR-128-3p formulations.
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http://dx.doi.org/10.1080/10717544.2020.1716882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054961PMC
December 2020

Econazole nitrate reversed the resistance of breast cancer cells to Adriamycin through inhibiting the PI3K/AKT signaling pathway.

Am J Cancer Res 2020 1;10(1):263-274. Epub 2020 Jan 1.

Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences Kunming, Yunnan, China.

Activation of the phosphoinositide 3 kinase (PI3K)/AKT pathway is frequently implicated in resistance to anticancer therapies. PI3K inhibitors can restore sensitivity to standard breast cancer therapies, including endocrine therapy, HER2-targeted agents, and chemotherapy. Our previous research showed that econazole, a novel PI3Ka inhibitor, inhibits the PI3K/AKT pathway and induces apoptosis in lung cancer cells. In this study, econazole showed significant cytotoxic activity against Adriamycin-resistant breast cancer cells and . Additionally, econazole significantly sensitized MDA-MB-231 and MCF-7 cells to Adriamycin via inhibiting the PI3K/AKT pathway. Overexpression of constitutively active AKT1 abolished the function of econazole. The combination of econazole and Adriamycin exerted synergistic inhibitory effects in breast cancer cells and . Taken together, the PI3K inhibitor econazole could effectively overcome Adriamycin resistance and showed synergistic effects with chemotherapy on breast cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017736PMC
January 2020

Area extraction and spatiotemporal characteristics of winter wheat-summer maize in Shandong Province using NDVI time series.

PLoS One 2019 12;14(12):e0226508. Epub 2019 Dec 12.

College of Information Science and Engineering, Shandong Agricultural University, Tai'an, Shan Dong, China.

The use of remote sensing to rapidly and accurately obtain information on the spatiotemporal distribution of large-scale wheat and maize acreage is of great significance for improving the level of food production management and ensuring food security. We constructed a MODIS-NDVI time series dataset, combined linear interpolation and the Harmonic Analysis of Time Series algorithm to smooth the time series data curve, and classified the data with random forest algorithms. The results show that winter wheat-summer maize planting areas were mainly distributed in the western plains, southern region, and north-eastern part of the middle mountainous regions while the eastern hilly regions were less distributed and scattered. The winter wheat-summer maize planting areas in the study area continued to grow from 2004-2016, with the most significant growth in the northern part of the western plains and Yellow River Delta. The spatial planting probability reflected the planting core area and showed an intensive planting pattern. During the study period, the peak value and time for the NDVI of the winter wheat were significantly different and showed an increasing trend, while these parameters for the summer maize were relatively stable with little change. Therefore, we mapped a spatial distribution of the winter wheat and summer maize, using the time series data pre-processing synthesis and phenology curve random forest classification methods. Through precision analysis, we obtained satisfactory results, which provided a straightforward and efficient method to monitor the winter wheat and summer maize.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0226508PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907822PMC
March 2020
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