Publications by authors named "Changzheng Tian"

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Propofol represses cell growth and metastasis by modulating the circNCAPG/miR-200a-3p/RAB5A axis in glioma.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

Department of Anesthesiology, The First Hospital of Hebei Medicine University, Shijiazhuang, Hebei,050011, P.R. China. Electronic address:

Background: Glioma is a common primary intracranial tumor, with high infiltration and aggression. Propofol (Pro) is associated with growth and metastasis in glioma. Meanwhile, circular RNA non-SMC condensin I complex subunit G (circNCAPG, hsa_circ_0007244) has been reported to be upregulated in glioma. This study is designed to explore the role and mechanism of circNCAPG in Pro-glioma progression.

Methods: Cell viability was determined by Cell Counting Kit-8 (CCK-8) assay. CircNCAPG, microRNA-200a-3p (miR-200a-3p), and member RAS oncogene family (RAB5A) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Colony number, apoptosis, migration, and invasion were analyzed by colony formation, flow cytometry, wound healing, and transwell assays. Matrix metallopeptidase 2 (MMP2), MMP9, and RAB5A protein levels were detected by western blot assay. The binding relationship between miR-200a-3p and circNCAPG or RAB5A was predicted by starbase 2.0, and then verified by a dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. The biological roles of circNCAPG and Pro on glioma tumor growth were examined by the xenograft tumor model in vivo.

Results: CircNCAPG and RAB5A expressed were upregulated, and miR-200a-3p was decreased in glioma tissues and cells, while their expression presented an opposite trend in Pro-treated glioma cells. Moreover, circNCAPG overexpression could abolish Pro-mediated proliferation, apoptosis, migration, and invasion in glioma cells in vitro. Mechanically, circNCAPG could regulate RAB5A expression by sponging miR-200a-3p. Pro blocked glioma tumor growth in vivo by modulating circNCAPG.

Conclusion: Pro could inhibit glioma cell growth and metastasis through the circNCAPG/miR-200a-3p/RAB5A axis, providing a promising therapeutic strategy for glioma treatment.
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http://dx.doi.org/10.1016/j.wneu.2021.06.036DOI Listing
June 2021
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