Publications by authors named "Changyu Liu"

56 Publications

Novel insights into the anaerobic digestion of propionate via Syntrophobacter fumaroxidans and Geobacter sulfurreducens: Process and mechanism.

Water Res 2021 Jul 19;200:117270. Epub 2021 May 19.

Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.

The accumulation of volatile fatty acids, particularly propionic acid, significantly inhibits the efficiency of the anaerobic digestion system. In propionate degradation metabolism, the unfavorable thermodynamics of syntrophic reactions, strict ecological niche of syntrophic priopionate oxidizing bacteria, and slow metabolic rate of methanogens are regarded as major limitations. In this study, Geobacter sulfurreducens was co-cultured with Syntrophobacter fumaroxidans in bioelelectrochemical cells to analyze the propionate degradation process, impact factor, mechanism metabolic pathways, and electron transfer comprehensively. The results revealed that the syntroph S. fumaroxidans and syntrophic partner G. sulfurreducens achieved more efficient propionate degradation than the control group, comprising S. fumaroxidans and methanogens. Moreover, the carbon resource concentration and pH were both significantly correlated with propionate degradation (P < 0.01). The results further confirmed that G. sulfurreducen strengthened the consumption of H and acetate via direct interspecific electron transfer in propionate degradation. These findings indicate that G. sulfurreducens plays an unidentified functional role in propionate degradation.
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http://dx.doi.org/10.1016/j.watres.2021.117270DOI Listing
July 2021

Influence of particle scattering on photo biochemical transformation process of direct absorption methane digester.

Bioresour Technol 2021 Feb 5;321:124460. Epub 2020 Dec 5.

College of Engineering, Northeast Agricultural University, Harbin 150030, PR China; Key Laboratory of Agricultural Renewable Resources Utilization Technology and Equipment in Cold Areas of Heilongjiang Province, Harbin 150030, PR China.

An outdoor anaerobic fermentation reactor loses a significant amount of energy due to heat dissipation to the surrounding environment. The digester of direct absorption biogas can effectively utilize solar energy and scattering of the medium to enhance reaction temperature, which can promote anaerobic fermentation of microorganisms. A numerical model for the direct absorption methane digester was established to investigate the mechanism of photo biochemical transformation. The average relative values of simulated results were 4.1% and 9.6%, indicating that the model can effectively simulate the heat transfer process of biogas slurry under solar irradiation. Decreasing the albedo and increasing the effect of forward scattering of small particles can improve the regenerative performance and biogas production of digester. Increasing the backward scattering effect of small particles limited biogas fermentation. Scattering distribution had bigger effects on the rates of biogas and propionic acid production than those of albedo.
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http://dx.doi.org/10.1016/j.biortech.2020.124460DOI Listing
February 2021

Actinomycin D causes oocyte maturation failure by inhibiting chromosome separation and spindle assembly†.

Biol Reprod 2021 01;104(1):94-105

Department of Obstetrics and Gynecology, Beijing Key laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China.

Actinomycin D (ActD) has been considered as one of the most effective and safe chemotherapeutic medications for treating a number of cancers. Although ActD has been used in the treatment of gynecological tumors and pediatric tumors for more than 50 years, the toxic effects of ActD on mammalian oocytes remain unknown. In this study, the influence of ActD on mouse and human oocyte maturation and the possible mechanisms were investigated. Notably, ActD inhibited oocyte maturation and arrested oocytes at the metaphase I (MI) stage in a dose-dependent manner. In addition, ActD arrested oocyte maturation when the oocytes were treated at different successive stages, including the germinal vesicle (GV), germinal vesicle breakdown, and MI stages. In ActD-treated oocytes, disordered chromosome condensation and irregular spindle assembly occurred, resulting in incomplete chromosome segregation and oocytes arresting at the MI phase; these results possibly occurred because ActD triggered the formation of reactive oxygen species, resulting in DNA damage and decreased ATP in mouse GV oocytes. Besides, in vivo treatment with ActD also inhibited mouse oocyte maturation. Similar effects were seen in human oocytes. Collectively, our results indicated that ActD exposure disrupted oocyte maturation by increasing DNA damage, which is a finding that might help with optimizing future methods for female fertility preservation before undergoing chemotherapy.
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http://dx.doi.org/10.1093/biolre/ioaa170DOI Listing
January 2021

Pulmonary contusion during the COVID-19 pandemic: challenges in diagnosis and treatment.

Surg Today 2020 Sep 22;50(9):1113-1116. Epub 2020 Jul 22.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan City, Hubei Province, China.

Managing patients with pulmonary contusion safely and effectively during the coronavirus disease 2019 (COVID-19) pandemic is challenging. This retrospective study analyzes the clinical data of 29 consecutive patients with pulmonary contusion, including two with COVID-19, at Tongji Hospital, Wuhan, China, in January and February, 2020. We analyzed the clinical manifestations, laboratory test results, computed tomography (CT) images, treatment, and clinical outcomes. The two patients with pulmonary contusion and COVID-19 had increased leukocyte and neutrophil counts, similar to the patients with pulmonary contusion alone. Interestingly, both these patients had subpleural ground glass opacity on CT images as a typical manifestation of COVID-19. All 29 patients were treated conservatively, including with closed thoracic drainage, instead of with thoracotomy. Six patients died of ARDS or craniocerebral injury, but the others stabilized. During the COVID-19 pandemic, patients with pulmonary contusion should be tested for SARS-CoV-2 and unless critical, thoracotomy should be avoided.
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http://dx.doi.org/10.1007/s00595-020-02081-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374948PMC
September 2020

[Comparison of Recovery Phase CT Features between Mild/moderate and Severe/critical Coronavirus Disease 2019 Patients].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2020 Jun;42(3):370-375

Department of Thoracic Surgery.

To investigate the computed tomographc(CT)features of mild/moderate and severe/critical cases of coronavirus disease 2019(COVID-19)in the recovery phase. Totally 63 discharged patients in Wuhan,China,who underwent both chest CT and reverse transcription-polymerase chain reaction(RT-PCR)from February 1 to February 29,2020,were included.With RT-PCR as a gold standard,the performance of chest CT in diagnosing COVID-19 was assessed.Patients were divided into mild/moderate and severe/critical groups according to the disease conditions,and clinical features such as sex,age,symptoms,hospital stay,comorbidities,and oxygen therapy were collected.CT images in the recovery phase were reviewed in terms of time from onset,CT features,location of lesions,lobe score,and total CT score. There were 37 patients in the mild/moderate group and 26 in the severe/critical group. Compared with the mild/moderate patients,the severe/critical patients had older age [(43±16) years (52±16) years; =2.10, =0.040], longer hospital stay [(15±6)d (19±7)d; =2.70, =0.009], higher dyspnea ratio (5.41% 53.85%; =18.90, <0.001), lower nasal oxygen therapy ratio (81.08% 19.23%;=23.66, <0.001), and higher bi-level positive airway pressure ventilation ratio (0 57.69%; =25.62, <0.001). Time from onset was (23±6) days in severe/critical group, significantly longer than that in mild/moderate group [(18±7) days] (=3.40, <0.001). Severe/critical patients had significantly higher crazy-paving pattern ratio (46.15% 10.81%;=4.24, =0.039) and lower ground-glass opacities ratio (15.38% 67.57%; =16.74, <0.001) than the mild/moderate patients. The proportion of lesions in peripheral lung was significantly higher in mild/moderate group than in severe/critical group (78.38% 34.61%; =13.43, <0.001), and the proportion of diffusely distributed lesions was significantly higher in severe/critical group than in mild/moderate group (65.38% 10.81%; =20.47, <0.001). Total CT score in severe/critical group was also significantly higher in severe/critical group than in mild/moderate group [11 (8,17) points 7 (4,9) points; =3.81, <0.001]. The CT features in the recovery stage differ between mild/moderate and severe/critical COVID-19 patients.The lung infiltration is remarkably more severe in the latter.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12691DOI Listing
June 2020

Estrogen promotes the metastasis of non‑small cell lung cancer via estrogen receptor β by upregulation of Toll‑like receptor 4 and activation of the myd88/NF‑κB/MMP2 pathway.

Oncol Rep 2020 Apr 2. Epub 2020 Apr 2.

Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Estrogen promotes non‑small cell lung cancer (NSCLC) metastasis via estrogen receptor β (ERβ)‑mediated invasiveness‑associated matrix metalloprotease 2 (MMP2) upregulation. However, how ERβ increases the aggressiveness of NSCLC cells remains unclear. Recently, MMP2 was found to be upregulated by Toll‑like receptor 4 (TLR4) signaling activation and to promote NSCLC metastasis. Our present study aimed to examine the role of ERβ in the activation of TLR4 signaling and in tumor progression and metastasis, and to explore the synergistic metastatic effect of a combination of ERβ and TLR4 activation on human NSCLC cells in vitro and in vivo. Here, we found that ERβ is associated with TLR4 in metastatic lymph nodes. Western blot analysis and immunofluorescence revealed that ERβ overexpression upregulated TLR4 protein expression and activated downstream targets, myeloid differentiation primary response 88 (myd88)/nuclear factor (NF)‑κB/MMP2, enhancing NSCLC cell migration and invasion in vitro. A novel ERβ‑TLR4 interaction in cell plasma was identified by co‑immunoprecipitation and confocal immunofluorescence. The combination of estradiol and specific TLR4 agonist lipopolysaccharide (LPS) synergistically promoted metastatic behaviors in NSCLC cells. In cell culture and murine lung metastasis models, exposure to estradiol and LPS induced increased matrix degradation and accelerated invadopodia and metastasis formation in NSCLC cells compared with that in cells treated with estradiol or LPS alone. Together, we showed that estrogen promoted NSCLC metastasis via ERβ by upregulating TLR4 and activating its downstream signaling axis myd88/NF‑κB/MMP2. The combined targeting of ERβ and TLR4 may be a novel therapeutic strategy against advanced metastatic lung cancer.
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http://dx.doi.org/10.3892/or.2020.7574DOI Listing
April 2020

Does the Nitrification-Suppressed BOD Test Make Sense?

Environ Sci Technol 2020 05 24;54(9):5323-5324. Epub 2020 Apr 24.

School of Environment and Science, Gold Coast Campus, Griffith University, Southport, Queensland 4222, Australia.

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http://dx.doi.org/10.1021/acs.est.0c00997DOI Listing
May 2020

Preparation, characterization and application of activated carbon from corn cob by KOH activation for removal of Hg(II) from aqueous solution.

Bioresour Technol 2020 Mar 6;306:123154. Epub 2020 Mar 6.

College of Engineering, Northeast Agriculture University, Harbin 150030, PR China; CAS Key Laboratory of Renewable Energy, Guangzhou Insitute of Energy Conversion, Guangzhou 510640, PR China; Key Laboratory of Agricultural Renewable Resources Utilization Technology and Equipment in Cold Areas of Heilongjiang Province, Harbin 150030, PR China. Electronic address:

In the present study, activated carbon was prepared from corn cob. Corn cob by potassium hydroxide activation. SEM, BET, Raman, FTIR and XPS analysis methods were used to characterize the physical and chemical properties of activated carbon. The effects of adsorbent dosage, adsorption time, pH and initial Hg(II) concentration on mercury ion removal rate were studied. The specific surface area of this material is 1054.2 m g. The Langmuir and Freundlich adsorption models were used to verify the adsorption isotherms. The adsorption isotherms were simulated well by the Langmuir model, which implied that it is a monolayer adsorption process. The kinetic data conformed to the pseudo-second-order model, which implied that the predominant process is chemisorption.
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http://dx.doi.org/10.1016/j.biortech.2020.123154DOI Listing
March 2020

NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis.

Cell Death Dis 2020 02 18;11(2):129. Epub 2020 Feb 18.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial hyperplasia, pannus formation, and cartilage and bone destruction. Nuclear receptor subfamily 1 group D member 1 (NR1D1) functions as a transcriptional repressor and plays a vital role in inflammatory reactions. However, whether NR1D1 is involved in synovial inflammation and joint destruction during the pathogenesis of RA is unknown. In this study, we found that NR1D1 expression was increased in synovial tissues from patients with RA and decreased in RA Fibroblast-like synoviocytes (FLSs) stimulated with IL-1β in vitro. We showed that NR1D1 activation decreased the expression of proinflammatory cytokines and matrix metalloproteinases (MMPs), while NR1D1 silencing exerted the opposite effect. Furthermore, NR1D1 activation reduced reactive oxygen species (ROS) generation and increased the production of nuclear transcription factor E2-related factor 2 (Nrf2)-associated enzymes. Mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) pathways were blocked by the NR1D1 agonist SR9009 but activated by NR1D1 silencing. NR1D1 activation also inhibited M1 macrophage polarization and suppressed osteoclastogenesis and osteoclast-related genes expression. Treatment with NR1D1 agonist SR9009 in collagen-induced arthritis (CIA) mouse significantly suppressed the hyperplasia of synovial, infiltration of inflammatory cell and destruction of cartilage and bone. Our findings demonstrate an important role for NR1D1 in RA and suggest its therapeutic potential.
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http://dx.doi.org/10.1038/s41419-020-2314-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028921PMC
February 2020

Graphitic Carbon Nitride (g-CN)-Derived Bamboo-Like Carbon Nanotubes/Co Nanoparticles Hybrids for Highly Efficient Electrocatalytic Oxygen Reduction.

ACS Appl Mater Interfaces 2020 Jan 16;12(4):4463-4472. Epub 2020 Jan 16.

State Key Laboratory of Electroanalytical Chemistry , Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun , Jilin 130022 , China.

The oxygen reduction reaction (ORR) is an extremely important reaction in many renewable energy-related devices. The sluggish kinetics of the ORR limits the development of many fuel cells. Design and synthesis of highly efficient nonprecious electrocatalysts are of vital importance for electrochemical reduction of oxygen. Herein, we develop a graphitic carbon nitride (g-CN)-derived bamboo-like carbon nanotubes/carbon-wrapped Co nanoparticles (BCNT/Co) electrocatalyst by a simple high-temperature pyrolysis and acid-leaching method. The catalytic performance of the as-designed electrocatalyst toward ORR outperforms the commercial Pt/C catalyst in alkaline solution. The onset potential of nonprecious BCNT/Co-800 catalyst was 1.12 V. The half-wave potential was 0.881 V. The result was superior to that of commercial Pt/C (0.827 V vs RHE). The Co nanoparticles, bamboo-like carbon nanotubes, defects, and Co-N active sites all result in the remarkable ORR activity, stability, and great methanol tolerance.
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http://dx.doi.org/10.1021/acsami.9b18454DOI Listing
January 2020

How to Identify the "LIVE/DEAD" States of Microbes Related to Biosensing.

ACS Sens 2020 01 8;5(1):258-264. Epub 2020 Jan 8.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun 130022 , PR China.

In this work, we fabricated a microbial biosensor with long-term stability, which relied on microbial activity. Activity of the microbe was commonly estimated by LIVE/DEAD assay and the propidium iodide (PI)-stained one was judged as dead. Herein, we proposed the utilization of a physiological state of microbes, which was neither live nor dead but between them. In this state, microbes represented a high PI-stained ratio but still had catalytic ability. This microbial state was obtained by forming the biofilm under the conditions of poor nutrition and low temperature. Thus, the dividing and proliferating ability of the microbes in the biofilm was weak, which was beneficial for long-term stability. This mechanism was further confirmed by the biosensors made from multifarious substrate materials, including graphene-based gel, biomass-based gel, graphite felt, and poly(vinyl chloride). This biosensor was applied to water pollution monitoring in the laboratory for 2 years and then was integrated into a multiparameter water quality monitoring station on a local lake for 2.5 years.
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http://dx.doi.org/10.1021/acssensors.9b02138DOI Listing
January 2020

Fe/N-doped hollow porous carbon spheres for oxygen reduction reaction.

Nanotechnology 2020 Mar 25;31(12):125404. Epub 2019 Nov 25.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China. University of Science and Technology of China, Hefei 230026, People's Republic of China.

Herein, we design a dual-template-assisted pyrolysis method to prepare ultra-small FeO nanoparticles anchored on Fe/N-doped hollow porous carbon spheres (0.010-Fe/NHPCS-800) for oxygen reduction reaction (ORR). The synthesized SiO nanospheres, which are selected as the hard template, contribute to forming macroporous structure. Pluronic ® F127 is employed to fabricate mesopores through high-temperature pyrolysis as a soft template. In this way, the 0.010-Fe/NHPCS-800 architecture represents an ordered hierarchically porous property with a large BET surface area (1812 m g), which can facilitate the mass transport of reactants and increase the electrochemically active area. The FeO nanoparticles wrapped by graphitic carbon layers provide more active sites, and the synergistic interaction between FeO nanoparticles and doping N has a positive effect on ORR performance. The 0.010-Fe/NHPCS-800 catalyst outperforms the most effective ORR activities among a series of Fe/NHPCS samples with onset potential of 0.95 V (versus reversible hydrogen potential) and half-wave potential of 0.81 V, which is almost the same as the commercial Pt/C (0.96 and 0.81 V, correspondingly) in 0.10 M KOH. However, both the stability and durability of 0.010-Fe/NHPCS-800 surpass those of commercial Pt/C. Given all these advantages, 0.010-Fe/NHPCS-800 is a promising candidate to take the place of Pt-based electrocatalysts for ORR in the future.
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http://dx.doi.org/10.1088/1361-6528/ab5b56DOI Listing
March 2020

Rac1 S71 Mediates the Interaction between Rac1 and 14-3-3 Proteins.

Cells 2019 08 30;8(9). Epub 2019 Aug 30.

Department of Medical Genetics, and Signal Transduction Research Group, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.

Both 14-3-3 proteins (14-3-3s) and Rho proteins regulate cytoskeleton remodeling and cell migration, which suggests a possible interaction between the signaling pathways regulated by these two groups of proteins. Indeed, more and more emerging evidence indicates the mutual regulation of these two signaling pathways. However, all of the data regarding the interaction between Rac1 signaling pathways and 14-3-3 signaling pathways are through either the upstream regulators or downstream substrates. It is not clear if Rac1 could interact with 14-3-3s directly. It is interesting to notice that the Rac1 sequence RPLSYP is likely a 14-3-3 protein binding motif following the phosphorylation of S71 by Akt. Thus, we hypothesize that Rac1 directly interacts with 14-3-3s. We tested this hypothesis in this research. By using mutagenesis, co-immunoprecipitation (co-IP), Rac1 activity assay, immunoblotting, and indirect immunofluorescence, we demonstrate that 14-3-3s interact with Rac1. This interaction is mediated by Rac1 S71 in both phosphorylation-dependent and -independent manners, but the phosphorylation-dependent interaction is much stronger. Epidermal growth factor (EGF) strongly stimulates the phosphorylation of Rac1 S71 and the interaction between 14-3-3s and Rac1. Mutating S71 to A completely abolishes both phosphorylation-dependent and -independent interactions between 14-3-3s and Rac1. The interaction between 14-3-3s and Rac1 mostly serve to regulate the activity and subcellular localization of Rac1. Among the seven 14-3-3 isoforms, 14-3-3η, -σ, and -θ showed interactions with Rac1 in both Cos-7 and HEK 293 cells. 14-3-3γ also binds to Rac1 in HEK 293 cells, but not in Cos-7 cells. We conclude that 14-3-3s interact with Rac1. This interaction is mediated by Rac1 S71 in both phosphorylation-dependent and -independent manners. The interaction between 14-3-3 and Rac1 mostly serves to regulate the activity and subcellular localization of Rac1. Among the seven 14-3-3 isoforms, 14-3-3η, -γ, -σ, and -θ interact with Rac1.
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http://dx.doi.org/10.3390/cells8091006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770128PMC
August 2019

Evaluation of methane production and energy conversion from corn stalk using furfural wastewater pretreatment for whole slurry anaerobic co-digestion.

Bioresour Technol 2019 Dec 19;293:121962. Epub 2019 Aug 19.

College of Engineering, Northeast Agriculture University, Harbin 150030, PR China; Key Laboratory of Agricultural Renewable Resources Utilization Technology and Equipment in Cold Areas of Heilongjiang Province, Harbin 150030, PR China. Electronic address:

In this study, corn stalk (CS) was pretreated with furfural wastewater (FWW) for whole slurry anaerobic digestion (AD), which increased the degradability of CS components, changed the parameters in pretreatment slurry and improved the biochemical methane potential (BMP). The ultimate goal was to optimize the time and temperature for FWW pretreatment and evaluate whether FWW pretreatment is feasible from BMP and energy conversion. The results of path analysis suggested that lignocellulosic degradability (LD) was the main factor affecting methane production with the comprehensive decision of 0.7006. The highest BMP (166.34 mL/g VS) was achieved by the pretreatment at 35 °C for 6 days, which was 70.36% higher than that of control check (CK) (97.64 mL/g VS) and the optimal pretreatment condition was predicted at 40.69 °C for 6.49 days by response surface methodology (RSM). The net residual value (NRV) for the pretreatment of 35 °C and 6 days was the highest (0.6201), which was the most appropriate condition for AD in real application.
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http://dx.doi.org/10.1016/j.biortech.2019.121962DOI Listing
December 2019

Impact of total carbon/sulfate on methane production and sulfate removal from co-digestion of sulfate-containing wastewater and corn stalk.

J Environ Manage 2019 Aug 16;243:411-418. Epub 2019 May 16.

Department of Agriculture Biological Environment and Energy Engineering, Northeast Agriculture University, No. 600 Changjiang Road, Xiangfang District, Harbin, 150030, China.

During the process of preparing furfural by straw depolymerization with dilute sulfuric acid, large amounts of high temperature sulfate-rich organic wastewater were produced. It cannot be treated directly by anaerobic digestion and converted to bioenergy due to high concentrations of sulfate. In this study, anaerobic co-digestion of sulfate containing wastewater and corn stalk was performed at thermophilic conditions to investigate the influences of total carbon (TC)/sulfate (6, 16, 35 and 110) on methane production and sulfate removal. The results showed that the highest methane production of 260.14 mL g volatile solid (VS) was achieved at TC/sulfate of 35, which was significantly higher than 12.53 mL g VS obtained at TC/sulfate of 6. Moreover, the results of sulfate balance analysis showed a maximum sulfate removal of 93.43% was achieved at TC/sulfate of 16, and sulfate concentration in biogas slurry was less than 0.1 g/L regardless of TC/sulfate after 28 days of co-digestion. The microbial community was analyzed using 16S rDNA sequencing technology, the results showed that methane was mainly produced by Methanoculleus and Methanosarcina, and sulfate was removed via Desulfotomaculum, and the relative abundance of methanogenic archaea (MA) and sulfate reducing bacteria (SRB) were significantly correlated with methane production and sulfate removal. It can concluded that higher methane production and sulfate removal can be obtained by anaerobic co-digestion of sulfate containing wastewater and corn stalk at properly TC/sulfate.
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http://dx.doi.org/10.1016/j.jenvman.2019.04.129DOI Listing
August 2019

Improved energy utilization efficiency via adding solar radiant heating mode for traditional bioreactor to dispose straw: Experimental and numerical evaluation.

Waste Manag 2019 Apr 15;89:303-312. Epub 2019 Apr 15.

College of Engineering, Northeast Agricultural University, Harbin 150030, China.

Energy utilization efficiency of heating for the operation process of biogas reactor is an important factor limiting its development and popularization. A novel mode of solar radiant heating combined with the conventional heating mode was proposed to reduce the power loss and improve the utilization cycle of heat exchanger. In present work, experimental and numerical researches about the anaerobic fermentation process under two heating modes were made to investigate the effect of temperature fluctuation on non-isothermal fermentation process under solar radiant heating. The results show that the methane production capacity of non-isothermal process under solar radiant heating reduces by up to 14% compared with the constant temperature condition in three seasons; increasing the total solid concentration of bioreactor is helpful for improving the effect of solar radiant heating; the effects of temperature fluctuation coefficient on acid and methane productions are bigger than the one on pH of slurry.
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http://dx.doi.org/10.1016/j.wasman.2019.04.027DOI Listing
April 2019

Tantalum nanoparticles reinforced polyetheretherketone shows enhanced bone formation.

Mater Sci Eng C Mater Biol Appl 2019 Aug 27;101:232-242. Epub 2019 Mar 27.

Department of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China. Electronic address:

Polyetheretherketone (PEEK) has been used in orthopedic surgery for several decades. Numerous methods were invented to alter the properties of PEEK. By adding nanoparticles, fibers, etc., elastic modulus and strength of PEEK can be changed to meet certain demand. In this study, tantalum (Ta), a promising metal, was introduced to modify the properties of PEEK, in which PEEK was reinforced with different contents of tantalum nanoparticles (from 1 wt% to 9 wt%). Mechanical properties and biological functions (both in vitro and in vivo) were then investigated. The highest elastic modulus and compressive strength were observed in 3%Ta-PEEK. Cell experiments as cell adhesion, collagen secretion, biomineralization and osteogenesis related gene expression showed preferable results in 3%Ta-PEEK and 5%Ta-PEEK. Improved bone integration was shown in 3%Ta-PEEK and 5%Ta-PEEK in vivo. Above all, enhanced mechanical properties and promoted bone formation were proved for 3%Ta-PEEK and 5%Ta-PEEK compared to others groups both in vitro and in vivo, suggesting that the addition of tantalum nanoparticles modified the osseointegration ability of PEEK. This composite of tantalum and PEEK could have a clinical potential for orthopedic implants.
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http://dx.doi.org/10.1016/j.msec.2019.03.091DOI Listing
August 2019

Polyarylene Ether Nitrile and Barium Titanate Nanocomposite Plasticized by Carboxylated Zinc Phthalocyanine Buffer.

Polymers (Basel) 2019 Mar 4;11(3). Epub 2019 Mar 4.

Research Branch of Advanced Functional Materials, School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, China.

Barium titanate (BT) and polyarylene ether nitrile (PEN) nanocomposites with enhanced dielectric properties were obtained by using carboxylatedzinc phthalocyanine (ZnPc-COOH) buffer as the plasticizer. Carboxylated zinc phthalocyanine, prepared through hydrolyzing ZnPc in NaOH solution, reacted with the hydroxyl groups on the peripheral of hydrogen peroxide treated BT (BT-OH) yielding core-shell structured [email protected] Thermogravimetric analysis (TGA), transmission electron microscopy (TEM), TEM energy dispersive spectrometer mapping, scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) demonstrated successful preparation of [email protected] The fabricated [email protected] was incorporated into the PEN matrix through the solution casting method. Rheological measurements demonstrated that the ZnPc-COOH buffer can improve the compatibility between BT and PEN effectively. With the existence of the ZnPc-COOH buffer, the prepared [email protected]/PEN nanocomposites exhibit a high dielectric constant of 5.94 and low dielectric loss (0.016 at 1000 Hz). [email protected]/PEN dielectric composite films can be easily prepared, presenting great application prospects in the field of organic film capacitors.
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http://dx.doi.org/10.3390/polym11030418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473433PMC
March 2019

Hesperetin suppresses RANKL-induced osteoclastogenesis and ameliorates lipopolysaccharide-induced bone loss.

J Cell Physiol 2019 07 11;234(7):11009-11022. Epub 2018 Dec 11.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Destructive bone diseases caused by osteolysis are increasing in incidence. They are characterized by an excessive imbalance of osteoclast formation and activation. During osteolysis, the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways are triggered by receptor activator of NF-κB ligand (RANKL), inflammatory factors, and oxidative stress. Previous studies have indicated that the common flavanone glycoside compound hesperetin exhibits anti-inflammatory and antioxidant activity by inhibition of NF-κB and MAPK signaling pathways. However, the direct relationship between hesperetin and osteolysis remain unclear. In the present study, we investigated the effects of hesperetin on lipopolysaccharide (LPS)-induced osteoporosis and elucidated the related mechanisms. Hesperetin effectively suppressed RANKL-induced osteoclastogenesis, osteoclastic bone resorption, and F-actin ring formation in a dose-dependent manner. It also significantly suppressed the expression of osteoclast-specific markers including tartrate-resistant acid phosphatase, matrix metalloproteinase-9, cathepsin K, c-Fos, and nuclear factor of activated T-cells cytoplasmic 1. Furthermore, it inhibited osteoclastogenesis by inhibiting activation of NF-κB and MAPK signaling, scavenging reactive oxygen species, and activating the nuclear factor E2 p45-related factor 2/heme oxygenase 1 (Nrf2/HO-1) signaling pathway. Consistent with in vitro results, hesperetin effectively ameliorated LPS-induced bone loss, reduced osteoclast numbers, and decreased the RANKL/OPG ratio in vivo. As such, our results suggest that hesperetin may be a great candidate for developing a novel drug for destructive bone diseases such as periodontal disease, tumor bone metastasis, rheumatoid arthritis, and osteoporosis.
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http://dx.doi.org/10.1002/jcp.27924DOI Listing
July 2019

Effects of Taxifolin on Osteoclastogenesis and .

Front Pharmacol 2018 12;9:1286. Epub 2018 Nov 12.

Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Osteoporosis is a highly prevalent disease which has been a major public health problem and considered to be associated with chronic low-grade systemic inflammation and oxidative damage. Taxifolin is a natural flavonoid and possesses many pharmacological activities including antioxidant and anti-inflammatory. Because flavonoids have been confirmed to fight osteoporosis and promote bone health, the aim of this study was to investigate the effects of taxifolin on the formation and function of osteoclast. In this study, we examined the effects of taxifolin on osteoclast using both and studies. Taxifolin suppressed the activation of nuclear factor-κB, C-Fos and mitogen-activated protein kinase, and also decreased osteoclast-specific genes expression, including , and . Taxifolin also prevented reactive oxygen species (ROS) production following RANKL stimulation. In addition, taxifolin alleviated ovariectomized-induced bone loss by repressing osteoclast activity and decreasing serum levels of tumor necrosis factor-α, interleukin-1β, interleukin-6 and receptor activator of nuclear factor-κB ligand (RANKL) . Our results indicated that taxifolin inhibits osteoclastogenesis via regulation of modulation of several RANKL signaling pathways. Therefore, taxifolin may be considered as a potential alternative therapeutic agent for treating osteoclast-related diseases.
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http://dx.doi.org/10.3389/fphar.2018.01286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240596PMC
November 2018

Preparation, performance, and application of a stable, sensitive and cost-effective microelectrode array.

Talanta 2018 Oct 25;188:245-250. Epub 2018 May 25.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China. Electronic address:

The development of rapid toxicity detection technology has higher requirements for electrode. In this work, a stable, sensitive and cost-effective microelectrode array (MEA) was successfully prepared manually. The advantage of the as-prepared MEA was discussed by means of detecting toxicity of 3,5-Dichlorophenol (3,5-DCP) by contrasting with bulk electrode and single microelectrode, in which mixed microorganisms were selected as biocatalyst, and K[Fe(CN)] was adopted as electron mediator. The current reached a stable state in 10 s under the constant potential of 450 mV. The feasibility of rapid detection of toxicity of formaldehyde with the MEA was further verified. The current responses were analyzed over formaldehyde of the final concentrations varied from 0.0036% to 1.0%, and the traditional parameter of 50% inhibitory concentration (IC) of 0.11% was obtained within 1 h. In brief, the as-prepared MEA has high sensitivity, good stability, strong anti-interference, and well corrosion resistance. It shows a very large application prospects in toxicity detection in water.
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http://dx.doi.org/10.1016/j.talanta.2018.05.081DOI Listing
October 2018

17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression.

J Exp Clin Cancer Res 2018 Jul 3;37(1):133. Epub 2018 Jul 3.

Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: In non-small cell lung cancer (NSCLC), estrogen (E2) significantly promotes NSCLC cell growth via estrogen receptor beta (ERβ). Discovery and elucidation of the mechanism underlying estrogen-promoted NSCLC progression is critical for effective preventive interventions. IL6 has been demonstrated to be involved in the development, progression and metastasis in several cancers and IL6 overexpression is associated with poor prognosis in NSCLC. However, the exact role played by IL6 in estrogen-promoted NSCLC progress remain unknown. Here, we evaluated the expression and biological effects of IL6 in NSCLC cells when treated with E2 and explored the underlying mechanism of IL6 in E2-promoted NSCLC progression.

Methods: Expression of ERβ/IL6 in 289 lung cancer samples was assessed by immunohistochemistry. Matched samples of metastatic lymph node and primary tumor tissues were used to quantify the expression of ERβ/IL6 by western blot. Expression levels of IL6 in NSCLC cells were quantified by western blotting, ELISA, and immunofluorescence staining. The effects of IL6 stimulated by E2 on cell malignancy were evaluated using CCK8, colony formation, wound healing and transwell. Furthermore, overexpression and knockdown ERβ constructs were constructed to measure the expression of IL6. The effects of IL6 stimulated by E2 on tumor growth were evaluated using a urethane-induced adenocarcinoma model. In addition, a xenograft mouse model was used to observe differences in ERβ subtype tumor growth with respect to IL6 expression.

Results: IL6/ERβ expression were significantly increased in lung cancer. Higher IL6/ERβ expression was associated with decreased differentiation or increased metastasis. IL6 was an independent prognostic factor for overall survival (OS), higher IL6 expression was associated with decreased OS. Furthermore, ERβ regulates IL6 expression via MAPK/ERK and PI3K/AKT pathways when stimulated by E2 and promotes cell malignancy in vitro and induced tumor growth in vivo. Finally we confirm that ERβ isolation 1/5 is essential for E2 promotion of IL6 expression, while ERβ2 not.

Conclusions: Our findings demonstrate that E2 stimulates IL6 expression to promote lung adenocarcinoma progression through the ERβ pathway. We also clarify the difference in each ERβ subtype for E2 promoting IL6 expression, suggesting that ERβ/IL6 might be potential targets for prognostic assessment and therapeutic intervention in lung cancer.
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http://dx.doi.org/10.1186/s13046-018-0804-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029357PMC
July 2018

Human nail stem cells are retained but hypofunctional during aging.

J Mol Histol 2018 Jun 9;49(3):303-316. Epub 2018 Apr 9.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Qiaokou District, Wuhan, 430030, China.

The nail is a continuous skin appendage. Cells located around the nails, which display coordinated homeostatic dynamics and release a flow of stem cells in response to regeneration, have been identified in mice. However, very few studies regarding human nail stem cells exist in the literature. Using specimens isolated from humans, we detected an unreported population of cells within the basal layer of postnatal human nail proximal folds (NPFs) and the nail matrix around the nail root. These cells were multi-expressing and expressed stem cell markers, such as keratin 15 (K15), keratin 14 (K14), keratin 19 (K19), CD29, CD34, and leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6). These cells were very similar to mouse nail stem cells in terms of cell marker expression and their location within the nail. We also found that the putative nail stem cells maintained their abundance with advancing age, but cell proliferation and nail growth rate were decreased on comparison of young and aged specimens. To summarize, we found a putative population of stem cells in postnatal human nails located at NPFs and the nail matrix. These cells may have potential for cell differentiation and be capable of responding to injury, and were retained, but may be hypofunctional during aging.
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http://dx.doi.org/10.1007/s10735-018-9769-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942356PMC
June 2018

Association between the Glutathione-S-transferase T1 null genotype and esophageal cancer susceptibility: a meta-analysis involving 11,163 subjects.

Oncotarget 2018 Mar 20;9(19):15111-15121. Epub 2018 Feb 20.

Department of Thoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.

Background: Glutathione-S-Transferase T1 (GSTT1) null genotype has been shown to be associated with the risk of esophageal cancer. However, the results remain inconsistent. Thus a comprehensive meta-analysis was conducted to assess the strength of association between GSTT1 null genotype and the risk of esophageal cancer.

Materials And Methods: A literature search of PubMed, Embase, China National Knowledge Infrastructure (CNKI) and Wanfang databases up to March 31, 2017 was conducted and 30 eligible articles with 4482 cases and 6681 controls were finally recruited. The strength of correlation between GSTT1 polymorphism and the susceptibility of esophageal cancer was assessed by the crude odds ratios (ORs) with 95% confidence intervals (CIs). Subgroup analyses and sensitivity analyses were performed to further identify the association.

Results: GSTT1 null genotype significantly increased the risk of esophageal cancer (OR = 1.20; 95% CI 1.04-1.40; < 0.05). In a subgroup analysis by ethnicity, GSTT1 null genotype was correlated with a significantly increased risk of esophageal cancer among Asians (OR = 1.33; 95% CI 1.12-1.58; < 0.05), instead of Caucasians or Africans (OR = 0.91; 95% CI 0.65-1.26; > 0.05 for Caucasians and OR = 1.32; 95% CI 0.98-1.77; > 0.05 for Africans). In the analysis by histological type, GSTT1 null genotype was correlated with a significantly increased risk of esophageal squamous cell carcinoma (OR = 1.34; 95% CI 1.12-1.61; < 0.05), particularly among Asians (OR = 1.54; 95% CI 1.30-1.82; < 0.05), but not among Caucasians or Africans (OR = 0.87; 95% CI 0.48-1.57; > 0.05 for Caucasians and OR = 1.32; 95% CI 0.98-1.77; > 0.05 for Africans). In addition, there is no significant correlation between GSTT1 null genotype and the risk of esophageal adenocarcinoma (OR = 0.98; 95% CI 0.71-1.35; > 0.05).

Conclusions: Our findings demonstrate that GSTT1 null genotype significantly increases esophageal cancer risk, particularly in Asians.
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http://dx.doi.org/10.18632/oncotarget.24534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871102PMC
March 2018

Single-Cell Transcriptomics of Human Oocytes: Environment-Driven Metabolic Competition and Compensatory Mechanisms During Oocyte Maturation.

Antioxid Redox Signal 2019 02 13;30(4):542-559. Epub 2018 Apr 13.

1 Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital , Beijing, China .

Aims: The mechanisms coordinating maturation with an environment-driven metabolic shift, a critical step in determining the developmental potential of human in vitro maturation (IVM) oocytes, remain to be elucidated. Here we explored the key genes regulating human oocyte maturation using single-cell RNA sequencing and illuminated the compensatory mechanism from a metabolic perspective by analyzing gene expression.

Results: Three key genes that encode CoA-related enzymes were screened from the RNA sequencing data. Two of them, ACAT1 and HADHA, were closely related to the regulation of substrate production in the Krebs cycle. Dysfunction of the Krebs cycle was induced by decreases in the activity of specific enzymes. Furthermore, the activator of these enzymes, the calcium concentration, was also decreased because of the failure of influx of exogenous calcium. Although release of endogenous calcium from the endoplasmic reticulum and mitochondria met the requirement for maturation, excessive release resulted in aneuploidy and developmental incompetence. High nicotinamide nucleotide transhydrogenase expression induced NADPH dehydrogenation to compensate for the NADH shortage resulting from the dysfunction of the Krebs cycle. Importantly, high NADP levels activated DPYD to enhance the repair of DNA double-strand breaks to maintain euploidy.

Innovation: The present study shows for the first time that exposure to the in vitro environment can lead to the decline of energy metabolism in human oocytes during maturation but that a compensatory action maintains their developmental competence.

Conclusion: In vitro maturation of human oocytes is mediated through a cascade of competing and compensatory actions driven by genes encoding enzymes.
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http://dx.doi.org/10.1089/ars.2017.7151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338670PMC
February 2019

YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.

Bone 2018 05 10;110:177-186. Epub 2018 Feb 10.

Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.. Electronic address:

Yes-associated protein 1 (YAP1), the core effector of the Hippo signaling pathway, has been identified as a key regulator of tissue homeostasis and organ development by controlling cell proliferation and differentiation. Previous studies have shown that YAP1 regulates multiple steps during skeletal development and bone remodeling, including the self-renewal and differentiation of mesenchymal stem cells (MSCs). However, its role in osteoclastogenesis remains largely unknown. Here, we report that YAP1 is an essential regulator for osteoclast differentiation and activity. Both mRNA and protein levels of YAP1 were downregulated during RANKL-induced osteoclastogenesis. Short hairpin RNA-mediated knockdown of YAP1 in bone marrow-derived macrophages (BMM) prevented the formation and function of multinucleated osteoclasts, and markedly abrogated the expression of osteoclast marker genes. Furthermore, the suppression of osteoclastogenesis and bone resorption activity were also observed in the BMM treated with verteporfin, a small molecule that inhibits the association of YAP1 with the transcriptional enhancer-associated domain (TEAD) family of transcription factors, the major partner of YAP1. Mechanistically, the interaction of YAP1/TEADs with AP-1 and cooperation on downstream gene transcription were confirmed, and RANKL-induced NF-κB signaling was also impaired in the YAP1-inhibited condition. Our results revealed the essential role of YAP1 and the YAP1-TEADs complex in regulating osteoclastogenesis and related gene expression.
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http://dx.doi.org/10.1016/j.bone.2018.01.035DOI Listing
May 2018

Carbon nanotube-modified oxidized regenerated cellulose gauzes for hemostatic applications.

Carbohydr Polym 2018 Mar 16;183:246-253. Epub 2017 Dec 16.

MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China.

Functionalized carbon nanotubes have recently received interest because of their unique properties, especially in the biomedical field. In this research, unmodified multiwalled carbon nanotubes (MWCNTs), and functionalized carbon nanotubes with amino groups (MWCNTs-NH) and carboxyl groups (MWCNTs-COOH) were grafted to oxidized regenerated cellulose (ORC) gauze to fabricate novel functionalized ORC, and the performance of the functionalized gauze was investigated. The functionalized ORC was characterized by FT-IR, XPS and SEM, which showed the different kinds of CNTs grafted on its surface. The XPS results demonstrated the successful incorporation of functionalized MWCNTs in the active layer of modified ORC gauze. Meanwhile, the specific surface area of the CNTs modified functionalized ORC gauze was improved in varying degrees, whereas the porosity was slightly decreased. Furthermore, hydrophilicity experiment results presented a significant increment in water uptake of the functionalized CNTs grafted to the surface of the ORC gauze. Results of the hemostatic performance test on rabbit ear artery and liver showed that compared with the original ORC gauze, the bleeding time was significantly reduced when using the functionalized CNTs modified ORC hemostatic gauze. Moreover, the results also showed that the MWCNTs-COOH/ORC functionalized gauze had outstanding hemostatic efficiency.
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http://dx.doi.org/10.1016/j.carbpol.2017.12.035DOI Listing
March 2018

Estrogen receptor β1 activation accelerates resistance to epidermal growth factor receptor-tyrosine kinase inhibitors in non-small cell lung cancer.

Oncol Rep 2018 Mar 4;39(3):1313-1321. Epub 2018 Jan 4.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related deaths worldwide. Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR‑TKIs) have revolutionized the treatment of patients with advanced EGFR-mutant NSCLC. However, drug resistance eventually develops in the majority of patients despite an excellent initial response. The present study aimed to investigate the mechanism of acquired resistance to EGFR-TKIs and to explore strategies to overcome the resistance to EGFR-TKIs from a gender perspective. PC9 and Hcc827 cell lines, sensitized to EGFR-TKI, and secondary TKI-resistant PC9-ER (erlotinib resistant) and Hcc827-ER cell lines were evaluated for the expression of ERβ1. The proliferative ability of both cell lines was analyzed after transfection of siRNA-ERβ1 using Cell Counting Kit-8 and colony formation assays. Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and Akt activation were detected. The co-inhibition efficiency of erlotinib and fulvestrant was analyzed in PC9-ER xenografts. The expression of ERβ1 was investigated in tumor tissues of EGFR-TKI-treated patients, and its correlation with clinicopathological factors and progression-free survival (PFS) was assessed. The expression of ERβ1 was upregulated secondary to EGFR-TKIs in PC9 and Hcc827 cell lines, with β-estradiol dependence. Both PC9-ER and Hcc827-ER cell lines were re-sensitized to erlotinib after downregulation of the expression of ERβ1. ERK1/2 and Akt pathways were activated following the silencing of the expression of ERβ1 in PC9-ER and Hcc827 cell lines. The co-treatment of erlotinib and fulvestrant exhibited better growth inhibitory efficiency compared with the treatment of each agent alone in PC9-ER-derived xenografts. Primary NSCLC samples of 53 patients treated with EGFR-TKIs were analyzed. ERβ1 was highly expressed, and the strong expression of cytoplasmic ERβ1 was related to a shorter PFS. In conclusion, ERβ1 was activated in EGFR-TKI secondary resistance. The downregulation of ERβ1 sensitized the cells to EGFR-TKIs. ERβ1 may be a key molecule in EGFR-TKI therapy. In addition, anti-ERβ1 treatment may reverse TKI resistance.
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http://dx.doi.org/10.3892/or.2018.6186DOI Listing
March 2018

Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Activity.

Front Pharmacol 2017 19;8:928. Epub 2017 Dec 19.

Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Dihydromyricetin (DMY), the main flavonoid component of , possesses pharmacological activities useful for treatment of diseases associated with inflammation and oxidative damage. Because osteoclasts are often involved in chronic low-grade systemic inflammation and oxidative damage, we hypothesized that DMY may be an effective treatment for osteoclast-related diseases. The effects of DMY on osteoclast formation and activity were examined . Female C57BL/6 mice were ovariectomized to mimic menopause-induced bone loss and treated with DMY, and femur samples were subjected to bone structure and histological analysis, serum biochemical indicators were also measured. DMY suppressed the activation of nuclear factor-κB, c-Fos and mitogen-activated protein kinase, and prevented production of reactive oxygen species. DMY decreased expression of osteoclast-specific genes, including , and . In addition, DMY prevented bone loss and decreased serum levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6, and with a decrease in the ratio between receptor activator of nuclear factor-κB (RANK) ligand (RANKL) and osteoprotegerin (OPG) . These findings demonstrate that DMY attenuates bone loss and inhibits osteoclast formation and activity through modulation of multiple pathways both upstream and downstream of RANKL signaling. DMY may thus be a useful option for treatment of osteoclast-related diseases such as rheumatoid arthritis and osteoporosis.
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http://dx.doi.org/10.3389/fphar.2017.00928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742133PMC
December 2017
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