Publications by authors named "Changning Li"

25 Publications

  • Page 1 of 1

Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions.

Sci Rep 2021 Mar 9;11(1):5525. Epub 2021 Mar 9.

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning, 530007, China.

As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum. Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.
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http://dx.doi.org/10.1038/s41598-021-85072-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943799PMC
March 2021

Effects of swine manure composting by microbial inoculation: Heavy metal fractions, humic substances, and bacterial community metabolism.

J Hazard Mater 2021 Mar 3;415:125559. Epub 2021 Mar 3.

Engineering Research Center for the Resource Utilization of Livestock and Poultry Wastes in Gansu Province, Lanzhou 730070, Gansu, China; College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, Gansu, China.

This study aimed to investigate the effect of swine manure composting with microbial inoculation (MI) and without MI (CK) on heavy metal (Cr, Cd, and Pb) fractions, humic substance (HS), and metabolism pathway. The results showed that MI could passivate the heavy metal Cr and reduce the proportion of exchangeable (EXC) fraction of Cd, but it does not affect the EXC fraction of Pb. Compared to CK, HS, humic acid (HA), and fulvic acid (FA) were significantly increased with MI at the maturity stage. The propagation of Proteobacteria (day 4) and Firmicutes (days 12 and 24) was strengthened with MI. Canonical correlation analysis found that HA and Firmicutes were positively correlated with heavy metal (Cr, Cd, and Pb) residual (RES) fraction, and FA was positively correlated with Proteobacteria. Moreover, MI can significantly increase amino acid metabolism and carbohydrate metabolism by day 4, enhance the metabolism of enzyme families and glycan biosynthesis by day 12, and improve membrane transport. Overall, MI could facilitates the increase in HA and FA content and transfer of heavy metal (Cr, Cd, and Pb) fractions, it particularly helps increase the RES fraction.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125559DOI Listing
March 2021

A cannabidiol-loaded Mg-gallate metal-organic framework-based potential therapeutic for glioblastomas.

J Mater Chem B 2021 03;9(10):2505-2514

Department of Chemical and Biological Engineering, University at Buffalo, USA. and RENEW Institute, University at Buffalo, USA.

Cannabidiol (CBD) has been shown to slow cancer cell growth and is toxic to human glioblastoma cell lines. Thus, CBD could be an effective therapeutic for glioblastoma. In the present study, we explored the anticancer effect of cannabidiol loaded magnesium-gallate (CBD/Mg-GA) metal-organic framework (MOF) using the rat glioma brain cancer (C6) cell line. Bioactive and microporous magnesium gallate MOF was employed for simultaneous delivery of two potential anticancer agents (gallic acid and CBD) to the cancer cells. Gallic acid (GA), a polyphenolic compound, is part of the MOF framework, while CBD is loaded within the framework. Slow degradation of CBD/Mg-GA MOF in physiological fluids leads to sustained release of GA and CBD. CBD's anti-cancer actions target mitochondria, inducing their dysfunction and generation of harmful reactive oxygen species (ROS). Anticancer effects of CBD/Mg-GA include a significant increase in ROS production and a reduction in anti-inflammatory responses as reflected by a significant decrease in TNF-α expression levels. Molecular mechanisms that underlie these effects include the modulation of NF-κB expression, triggering the apoptotic cascades of glioma cells. CBD/Mg-GA MOF has potential anti-cancer, anti-inflammatory and anti-oxidant properties. Thus, the present study demonstrates that CBD/Mg-GA MOF may be a promising therapeutic for glioblastoma.
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http://dx.doi.org/10.1039/d0tb02780dDOI Listing
March 2021

Copper@ZIF-8 Core-Shell Nanowires for Reusable Antimicrobial Face Masks.

Adv Funct Mater 2020 Dec 13:2008054. Epub 2020 Dec 13.

Department of Chemical and Biological Engineering University at Buffalo (SUNY) Buffalo New York 14260 USA.

SARS-CoV-2 and other respiratory viruses spread via aerosols generated by infected people. Face masks can limit transmission. However, widespread use of disposable masks consumes tremendous resources and generates waste. Here, a novel material for treating blown polypropylene filtration media used in medical-grade masks to impart antimicrobial activity is reported. To produce thin copper@ZIF-8 core-shell nanowires (Cu@ZIF-8 NWs), Cu NWs are stabilized using a pluronic F-127 block copolymer, followed by growth of ZIF-8 to obtain uniform core-shell structures. The Cu@ZIF-8 NWs are applied to filtration media by dip coating. Aerosol filtration efficiency decreases upon exposure to ethanol (solvent for dip-coating), but increases with addition of Cu@ZIF-8 NWs. Cu@ZIF-8 NWs shows enhanced antibacterial activity, compared to Cu NWs or ZIF-8 alone, against and . Antiviral activity against SARS-CoV-2 is assayed using virus-infected Vero E6 cells, demonstrating 55% inhibition of virus replication after 48 h by 1 µg of Cu@ZIF-8 NWs per well. Cu@ZIF-8 NWs' cytotoxicity is tested against four cell lines, and their effect on inflammatory response in A549 cells is examined, demonstrating good biocompatibility. This low-cost, scalable synthesis and straightforward deposition of Cu@ZIF-8 NWs onto filter media has great potential to reduce disease transmission, resource consumption, and environmental impact of waste.
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http://dx.doi.org/10.1002/adfm.202008054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883136PMC
December 2020

The Association Between Health Literacy and Self-rated Health Among Residents of China Aged 15-69 Years.

Am J Prev Med 2021 04 12;60(4):569-578. Epub 2021 Feb 12.

Division of Surveillance and Evaluation, Chinese Center for Health Education, Beijing, China.

Introduction: Health literacy is a critical determinant of health. However, the association between health literacy and outcomes among Chinese residents has not been studied using nationally representative data. This study examines the association between health literacy and self-rated health among Chinese residents based on the 2017 China Health Literacy Survey.

Methods: The 2017 China Health Literacy Survey was conducted among non-institutionalized residents aged 15-69 years from 31 provinces in China. Self-rated health was measured using a single question with a 1-5 scale. Health literacy was assessed using the Chinese Health Literacy Scale. Multilevel linear regression models examined the association between health literacy and self-rated health. Data were collected in 2017 and analyzed between 2018 and 2019.

Results: The final sample size was 85,384. The overall weighted mean of the self-rated health score was 4.02 (95% CI=4.00, 4.03). After adjusting for individual-, county-, and province-level covariates, Chinese residents with higher levels of health literacy were more likely to have better self-rated health (β=0.0007, SE=0.0002, p<0.001). Of 6 dimensions of health literacy, 4 (i.e., infectious diseases literacy, chronic diseases literacy, medical care literacy, and health information literacy) were associated with self-rated health. Additionally, self-rated health was associated with gender, age, education, occupation, annual household income, and chronic conditions.

Conclusions: Using nationally representative data collected in 2017, this study found that self-rated health is associated with health literacy among Chinese residents aged 15-69 years. The promotion of health literacy should be an important component of health education, patient management, and health promotion.
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http://dx.doi.org/10.1016/j.amepre.2020.05.032DOI Listing
April 2021

A 3D-printed molecular ferroelectric metamaterial.

Proc Natl Acad Sci U S A 2020 Nov 19;117(44):27204-27210. Epub 2020 Oct 19.

Department of Mechanical and Aerospace Engineering, The State University of New York at Buffalo, Buffalo, NY 14260;

Molecular ferroelectrics combine electromechanical coupling and electric polarizabilities, offering immense promise in stimuli-dependent metamaterials. Despite such promise, current physical realizations of mechanical metamaterials remain hindered by the lack of rapid-prototyping ferroelectric metamaterial structures. Here, we present a continuous rapid printing strategy for the volumetric deposition of water-soluble molecular ferroelectric metamaterials with precise spatial control in virtually any three-dimensional (3D) geometry by means of an electric-field-assisted additive manufacturing. We demonstrate a scaffold-supported ferroelectric crystalline lattice that enables self-healing and a reprogrammable stiffness for dynamic tuning of mechanical metamaterials with a long lifetime and sustainability. A molecular ferroelectric architecture with resonant inclusions then exhibits adaptive mitigation of incident vibroacoustic dynamic loads via an electrically tunable subwavelength-frequency band gap. The findings shown here pave the way for the versatile additive manufacturing of molecular ferroelectric metamaterials.
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http://dx.doi.org/10.1073/pnas.2013934117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959491PMC
November 2020

Reducing Salt Intake in China with "Action on Salt China" (ASC): Protocol for Campaigns and Randomized Controlled Trials.

JMIR Res Protoc 2020 Apr 9;9(4):e15933. Epub 2020 Apr 9.

Chinese Center for Disease Control and Prevention, Beijing, China.

Background: Salt intake in China is over twice the maximum recommendation of the World Health Organization. Unlike most developed countries where salt intake is mainly derived from prepackaged foods, around 80% of the salt consumed in China is added during cooking.

Objective: Action on Salt China (ASC), initiated in 2017, aims to develop, implement, and evaluate a comprehensive and tailored salt reduction program for national scaling-up.

Methods: ASC consists of six programs working in synergy to increase salt awareness and to reduce the amount of salt used during cooking at home and in restaurants, as well as in processed foods. Since September 2018, two health campaigns on health education and processed foods have respectively started, in parallel with four open-label cluster randomized controlled trials (RCTs) in six provinces across China: (1) app-based intervention study (AIS), in which a mobile app is used to achieve and sustain salt reduction in school children and their families; (2) home cook-based intervention study (HIS), in which family cooks receive support in using less salt; (3) restaurant-based intervention study (RIS) targeting restaurant consumers, cooks, and managers; and (4) comprehensive intervention study (CIS), which is a real-world implementation and evaluation of all available interventions in the three other RCTs. To explore the barriers, facilitators, and effectiveness of delivering a comprehensive salt reduction intervention, these RCTs will last for 1 year (stage 1), followed by nationwide implementation (stage 2). In AIS, HIS, and CIS, the primary outcome of salt reduction will be evaluated by 24-hour urinary sodium excretion in 6030 participants, including 5436 adults and 594 school children around 8-9 years old. In RIS, the salt content of meals will be measured by laboratory food analysis of the 5 best-selling dishes from 192 restaurants. Secondary outcomes will include process evaluation; changes in knowledge, attitude, and practice on salt intake; and economic evaluation.

Results: All RCTs have been approved by Queen Mary Research Ethics Committee and the Institutional Review Boards of leading institutes in China. The research started in June 2017 and is expected to be completed around March 2021. The baseline investigations of the four RCTs were completed in May 2019.

Conclusions: The ASC project is progressing smoothly. The intervention packages and tailored components will be promoted for salt reduction in China, and could be adopted by other countries.

Trial Registration: Chinese Clinical Trial Registry. AIS: ChiCTR1800017553; https://tinyurl.com/vdr8rpr. HIS: ChiCTR1800016804; https://tinyurl.com/w8c7x3w. RIS: ChiCTR1800019694; https://tinyurl.com/uqkjgfw. CIS: ChiCTR1800018119; https://tinyurl.com/s3ajldw.

International Registered Report Identifier (irrid): DERR1-10.2196/15933.
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http://dx.doi.org/10.2196/15933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180507PMC
April 2020

An All-Ceramic, Anisotropic, and Flexible Aerogel Insulation Material.

Nano Lett 2020 May 10;20(5):3828-3835. Epub 2020 Apr 10.

Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, New York 14260, United States.

To exploit the high-temperature superinsulation potential of anisotropic thermal management materials, the incorporation of ceramic aerogel into the aligned structural networks is indispensable. However, the long-standing obstacle to exploring ultralight superinsulation ceramic aerogels is the inaccessibility of its mechanical elasticity, stability, and anisotropic thermal insulation. In this study, we report a recoverable, flexible ceramic fiber-aerogel composite with anisotropic lamellar structure, where the interfacial cross-linking between ceramic fiber and aerogel is important in its superinsulation performance. The resulting ultralight aerogel composite exhibits a density of 0.05 g/cm, large strain recovery (over 50%), and low thermal conductivity (0.0224 W m K), while its hydrophobicity is achieved by in situ trichlorosilane coating with the water contact angle of 135°. The hygroscopic tests of such aerogel composites demonstrate a reversible thermal insulation. The mechanical elasticity and stability of the anisotropic composites, with its soundproof performance, shed light on the low-cost superelastic aerogel manufacturing with scalability for energy saving building applications.
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http://dx.doi.org/10.1021/acs.nanolett.0c00917DOI Listing
May 2020

Effects of microbial inoculation on enzyme activity, available nitrogen content, and bacterial succession during pig manure composting.

Bioresour Technol 2020 Mar 9;306:123167. Epub 2020 Mar 9.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.

This study evaluated enzyme activity, available nitrogen, and bacterial succession during pig manure composting with and without microbial inoculation (ABB and CK, respectively). ABB reached the thermophilic stage 2 days than CK. Cellulose, urease, phosphatase, and sucrase activities were higher in ABB than in CK on days 12-24 of composting, but catalase activity was lesser in ABB than in CK throughout composting. NH-N and NO-N were significantly increased in ABB at the maturity stage. 16S rRNA sequencing revealed Nocardiopsaceae, Bacillaceae, Streptosporangiaceaec, Flavobacteriaceae, and Caldicoprobacteraceae as the dominant bacteria at the family level. Metabolism function analysis revealed that human diseases were reduced and carbohydrate metabolism was increased in ABB. Correlation analysis revealed that urease, sucrose, and phosphatase were significantly correlated with bacteria at the species level, whereas NH-N and NO-N were not significantly correlated. These results indicated that microbial inoculation accelerated the composting process and significantly regulated microbial functions.
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http://dx.doi.org/10.1016/j.biortech.2020.123167DOI Listing
March 2020

Genome-Wide Analysis of the DREB Subfamily in Reveals Their Functional Divergence During Cold and Drought Stresses.

Front Genet 2019 5;10:1326. Epub 2020 Feb 5.

Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Nanning, China.

Drought and cold stresses are the main environmental factors that affect the yield of sugarcane, and genes play very important roles in tolerance to drought, cold, and other environmental stresses. In this study, bioinformatics analysis was performed to characterize genes. RNA sequencing (RNA-seq) was used to detect the expression profiles of induced by cold and drought stresses. According to our results, there are 110 SsDREB subfamily proteins in , which can be classified into six groups; 106 of these genes are distributed among 29 chromosomes. Inter- and intraspecies synteny analyses suggested that all DREB groups have undergone gene duplication, highlighting the polyploid events that played an important role in the expansion of the DREB subfamily. Furthermore, RNA-seq results showed that 45 were up- or downregulated under cold stress; 35 of them were found to be involved in responding to drought stress. According to protein-protein interaction analysis, SsDREB100, SsDREB102, and SsDREB105 play key roles during the response to cold stress. These results reveal that functional divergence exists between collinear homologous genes or among common origin genes in the DREB subfamily of . This study presents a comprehensive analysis and systematic understanding of the precise mechanism of in response to abiotic stress and will lead to improvements in sugarcane.
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http://dx.doi.org/10.3389/fgene.2019.01326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013043PMC
February 2020

A Hierarchical Mesoporous Insulation Ceramic.

Nano Lett 2020 Feb 3;20(2):1110-1116. Epub 2020 Jan 3.

Department of Mechanical and Aerospace Engineering , University at Buffalo, The State University of New York , Buffalo , New York 14260 , United States.

Light-weight ceramic aerogels hold promise for superinsulation. However, its mechanical instability and complex manufacturing hampered its technical applications. In this study, we demonstrate lightweight pore-gradient ceramic aerogel-like foam monoliths (PGAFoams) through one-pot and in situ bubble supported pore gradient formation. The mechanically strong PGAFoams exhibit a low thermal conductivity of 0.036 W m K and a compressive strength of 89.85 MPa. The pore gradient and integral ceramic monolith nature provides such hydrophobic PGAFoams with thermal management, robust soundproof, and fire-resistance performance. Highly machinable PGAFoams can be adapted into a variety of shapes and dimensions to accommodate complex geometry applications. The scalable manufacturing of lightweight PGAFoams opens up building insulation with remarkable thermal management, high mechanical strength, low mass density, superior soundproofing, and fire-retardant performances.
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http://dx.doi.org/10.1021/acs.nanolett.9b04411DOI Listing
February 2020

Evaluation of seven chemical pesticides by mixed microbial culture (PCS-1): Degradation ability, microbial community, and Medicago sativa phytotoxicity.

J Hazard Mater 2020 05 5;389:121834. Epub 2019 Dec 5.

College of Grassland Science, Gansu Agricultural University, Lanzhou, China; Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Ministry of Education, Lanzhou, China.

Environmental problems caused by the large-scale use of chemical pesticides are becoming more and more serious, and the removal of chemical pesticides from the ecological environment by microbial degradation has attracted wide attention. In this study, using enrichment screening with seven chemical pesticides as the sole carbon source, a mixed microbial culture (PCS-1) was obtained from the continuous cropping of strawberry fields. The microbial community composition, degradation ability, and detoxification effect of PCS-1 was determined for the seven pesticides. Inoculation with PCS-1 showed significant degradation of and tolerance to the seven pesticides. Microbial community composition analysis indicated that Pseudomonas, Enterobacter, Aspergillus, and Rhodotorula were the dominant genera for the degradation of the seven pesticides by PCS-1. The concentration of the seven pesticides was 10 mg L in hydroponic and soil culture experiments. The fresh weight, plant height, and root length of PCS-1-inoculated alfalfa (Medicago sativa) significantly increased compared with those of non-PCS-1-inoculated M. sativa. PCS-1 not only effectively degraded the residual content of the seven pesticides in water and soil but also reduced the pesticide residues in the roots, stems, and leaves of M. sativa. This study shows that PCS-1 may be important in environmental remediation involving the seven pesticides.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121834DOI Listing
May 2020

Magnetically active transition metal cation-substituted alumina.

Nanotechnology 2020 Mar 21;31(10):105703. Epub 2019 Nov 21.

Department of Mechanical and Aerospace Engineering, Department of Chemistry, Research and Education in Energy Environment and Water (RENEW) Institute, University at Buffalo, The State University of New York, Buffalo, NY 14260, United States of America.

Alumina (AlO) is one of the most widely used ceramic materials for innumerable applications, due to its unique combination of attractive physical and mechanical properties. These intrinsic properties are dictated by the numerous phases that AlO forms and its related phase transformations. Transition metal (TM) cation dopants (iron (Fe), cobalt (Co), nickel (Ni) and manganese (Mn)), even in sparse amounts, have been shown to significantly affect the phase transformation and microstructural evolution of AlO. Small concentrations of TM cation dopants have successfully been incorporated to synthesize magnetically active AlO, while reducing the θ to α phase transformation temperature by 150 °C, and maintaining the outstanding mechanical properties. In addition, first-principle calculations based on density-functional theory with hybrid functional (HSE06) and the PBE+U methods have provided a mechanistic understanding of the formation energy and magnetism of the TM-doped α and θ phases of AlO. The results reveal a potential route for phase transition regulation and external magnetic field-induced texturing of AlO ceramics.
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http://dx.doi.org/10.1088/1361-6528/ab59faDOI Listing
March 2020

Correlation at two-dimensional charge-transfer FeSe interface.

Chem Commun (Camb) 2019 Oct;55(84):12643-12646

Department of Mechanical and Aerospace Engineering, Research and Education in Energy Environment & Water (RENEW) Institute, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.

The charge transfer and spin coupling effects are explored at the interface of two-dimensional (2D) superconducting FeSe nanosheets and molecular photochromic potassium-7,7,8,8-tetracyanoquinodimethane (KTCNQ). Light-induced conductivity in 2D FeSe nanosheets is enhanced by the electron doping from KTCNQ by the destabilized spin-Peierls phase through their interface. Furthermore, the spin coupling at the interface of FeSe and KTCNQ shifts the dimerization transition temperature of KTCNQ. Our results suggest 2D exfoliated FeSe nanosheets as a versatile strongly correlated platform for the study of interfacial electron doping and spin coupling.
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http://dx.doi.org/10.1039/c9cc06163kDOI Listing
October 2019

Surfactant-Stripped Micelles for NIR-II Photoacoustic Imaging through 12 cm of Breast Tissue and Whole Human Breasts.

Adv Mater 2019 Oct 15;31(40):e1902279. Epub 2019 Aug 15.

Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.

Surfactant-stripped micelles are formed from a commercially available cyanine fluoroalkylphosphate (CyFaP) salt dye and used for high contrast photoacoustic imaging (PAI) in the second near-infrared window (NIR-II). The co-loading of Coenzyme Q10 into surfactant-stripped CyFaP (ss-CyFaP) micelles improves yield, storage stability, and results in a peak absorption wavelength in the NIR-II window close to the 1064 nm output of Nd-YAG lasers used for PAI. Aqueous ss-CyFaP dispersions exhibit intense NIR-II optical absorption, calculated to be greater than 500 at 1064 nm. ss-CyFaP is detected through 12 cm of chicken breast tissue with PAI. In preclinical animal models, ss-CyFaP is visualized in draining lymph nodes of rats through 3.1 cm of overlaid chicken breast tissue. Following intravenous administration, ss-CyFaP accumulates in neoplastic tissues of mice and rats bearing orthotopic mammary tumors without observation of acute toxic side effects. ss-CyFaP is imaged through whole compressed human breasts in three female volunteers at depths of 2.6-5.1 cm. Taken together, these data show that ss-CyFaP is an accessible contrast agent for deep tissue PAI in the NIR-II window.
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http://dx.doi.org/10.1002/adma.201902279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773519PMC
October 2019

Microbial inoculation influences bacterial community succession and physicochemical characteristics during pig manure composting with corn straw.

Bioresour Technol 2019 Oct 13;289:121653. Epub 2019 Jun 13.

College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, Gansu, China; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.

This study determined the physicochemical changes and bacterial community succession in the pig manure composting process with microbial inoculant. Microbial inoculant could prolong the thermophilic stage by 2 days and increased the germination index (GI). Analysis with 16S rDNA showed that the Chao1 and Shannon indices increased at the thermophilic stage in the treatment (T), while those of the control (C) decreased. Microbial inoculant increased the relative abundance of Flavobacterium and Solibacillus in 4-12 and 12-24 days, respectively. Acinetobacter was reduced at 4-12 days. The key physicochemical factors affecting microbial successions were revealed by canonical correspondence analysis (CCA) and correlation analysis. Linear discriminant analysis (LDA) effect size (LEfse) analysis showed that there were 78 biomarkers, while in piles T and C, there were 35 and 43 biomarkers, respectively. These results indicated the addition of microbial inoculant improved the maturity and fertility, as well as significantly regulating the microbial community structure.
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http://dx.doi.org/10.1016/j.biortech.2019.121653DOI Listing
October 2019

Comparative transcriptome profiling of resistant and susceptible sugarcane genotypes in response to the airborne pathogen Fusarium verticillioides.

Mol Biol Rep 2019 Aug 20;46(4):3777-3789. Epub 2019 Apr 20.

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Science, Nanning, 530007, China.

Fusarium verticillioides is the pathogen associated with pokkah boeng disease (PBD), the most significant airborne disease of sugarcane. The molecular mechanisms that regulate the defense responses of sugarcane towards this fungus are not yet fully known. Samples of 'YT 94/128' (resistant, R) and 'GT 37' (susceptible, S) inoculated with F. verticillioides on the 14 days post-inoculation were used to analyze the transcriptome to screen R genes. In total, 80.93 Gb of data and 76,175 Unigenes were obtained after assembling the sequencing data, and comparisons of Unigenes with NR, Swiss-prot, KOG, and KEGG databases confirmed 42,451 Unigenes. The analysis of differentially expression genes (DEGs) in each sample revealed 9092 DEGs in 'YT 94/128,' including 8131 up-regulated DEGs and 961 down-regulated DEGs; there were 9829 DEGs in 'GT 37,' including 7552 up-regulated DEGs and 2277 down-regulated DEGs. The identified DEGs were mainly involved in catalytic enzyme activity, cell protease, hydrolytic enzymes, peptide enzyme, protein metabolism process of negative regulation, phenylpropanoid metabolism, extracellular region, aldehyde dehydrogenase, endopeptidase, REDOX enzyme, protein kinases, and phosphoric acid transferase categories. KEGG pathway clustering analysis showed that the DEGs involved in resistance were significantly related to metabolic pathways of phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, nitrogenous metabolism, biosynthesis of secondary metabolites, and plant-pathogen interactions. This application of transcriptomic data clarifies the mechanism of interactions between sugarcane and F. verticillioides, which can help to reveal disease-related metabolic pathways, molecular regulatory networks, and key genes involved in sugarcane responses to F. verticillioides.
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http://dx.doi.org/10.1007/s11033-019-04820-9DOI Listing
August 2019

Exciton-dipole coupling in two-dimensional rubrene assembly sensors.

Nanoscale 2019 Mar;11(12):5640-5645

Department of Mechanical and Aerospace Engineering, University at buffalo, The State University of New York, Buffalo, NY 14260, USA.

The selective detection of molecules with less energy consumption depends critically on novel sensing concepts and the emergence of new sensor materials. Excitons and dipole moments are two strongly correlated states that have shown coupled electronic interactions on the nanoscale, and they are highly sensitive to changes in their surroundings. Here, we present exciton-dipole coupling in the two-dimensional (2D) assembly of molecular rubrene excitonic crystals to selectively detect molecules. The presence of molecules with a dipole moment transforms excitons into charge transfer, resulting in a pronounced conductivity change of freestanding rubrene nanosheets. The exciton-dipole coupling exhibits highly efficient molecular selectivity, as it offers an unambiguous electronic fingerprint for the detection of molecules - in contrast to common sensing schemes relying on the quantification of intensity changes and optical peak shifts.
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http://dx.doi.org/10.1039/c8nr10373aDOI Listing
March 2019

Strongly Correlated Aromatic Molecular Conductor.

Small 2019 Apr 20;15(14):e1900299. Epub 2019 Feb 20.

Department of Mechanical and Aerospace Engineering, Research and Education in Energy, Environment & Water (RENEW) Institute, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.

Strongly correlated electronic molecules open the way for strong coupling between charge, spin, and lattice degrees of freedom to enable interdisciplinary fields, such as molecular electronic switches and plasmonics, spintronics, information storage, and superconducting circuits. However, despite exciting computational predictions and promising advantages to prepare flexible geometries, the electron correlation effect in molecules has been elusive. Here, the electron correlation effects of molecular plasmonic films are reported to uncover their coupling of charge, spin, lattice, and orbital for the switchable metal-to-insulator transition under external stimuli, at which the simultaneous transition occurs from the paramagnetic, electrical, and thermal conducting state to the diamagnetic, electrical, and thermal insulating state. In addition, density functional theory calculation and spectroscopic studies are combined to provide the mechanistic understanding of electronic transitions and molecular plasmon resonance observed in molecular conducting films. The self-assembled molecular correlated conductor paves the way for the next generation integrated micro/nanosystems.
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http://dx.doi.org/10.1002/smll.201900299DOI Listing
April 2019

Eutectic melt crystallization of L1-FePt.

Chem Commun (Camb) 2019 Jan;55(5):656-658

aDepartment of Mechanical and Aerospace Engineering, and Research and Education in Energy, Environment & Water (RENEW) Institute, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.

Herein, we report the eutectic growth control of ordered L10-FePt, which directs the nucleation, growth and crystallization of FePt sheets in a single-step reaction. The nature of eutectic crystallization at the eutectic point suggests its role as a high-temperature solvent, exhibiting an advantage in the scale-up production of metastable alloys.
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http://dx.doi.org/10.1039/c8cc08199aDOI Listing
January 2019

Magnetoelectric Radical Hydrocarbons.

Adv Mater 2019 Jan 20;31(3):e1806263. Epub 2018 Nov 20.

Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.

The molecular radicals, systems with unpaired electrons of open-shell electronic structures, set the stage for a multidisciplinary science frontier relevant to the cooperative magnetic exchange interaction and magnetoelectric effect. Here ferroelectricity together with magnetic spin exchange coupling in molecular radical hydrocarbon solids is reported, representing a new class of magnetoelectrics. Electronic correlation through radical-radical interactions plays a decisive role in the coupling between magnetic and charge orders. A substantial photoconductance and visible-light photovoltaic effect are found in radical hydrocarbons. The ability to simultaneously control and retrieve the changes in magnetic and electrical responses opens up a new breadth of applications, such as radical magnetoelectrics, magnets, and optoelectronics.
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http://dx.doi.org/10.1002/adma.201806263DOI Listing
January 2019

Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.).

Sci Rep 2017 12 15;7(1):17659. Epub 2017 Dec 15.

College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi, 530004, China.

In plants, both abscisic acid (ABA) dependent and independent pathways form the basis for the response to environmental stresses. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) plays a central role in plant stress signal transduction. However, complete annotation and specific expression patterns of SnRK2s in sugarcane remain unclear. For the present study, we performed a full-length cDNA library survey of sugarcane, thus identifying ten SoSnRK2 genes via phylogenetic, local BLAST methods, and various bioinformatics analyses. Phylogenetic analysis indicated division of SoSnRK2 genes into three subgroups, similar to other plant species. Gene structure comparison with Arabidopsis suggested a unique evolutionary imprint of the SnRK2 gene family in sugarcane. Both sequence alignment and structural annotation provided an overview of the conserved N-terminal and variations of the C-terminal, suggesting functional divergence. Transcript and transient expression assays revealed SoSnRK2s to be involved in the responses to diverse stress signals, and strong ABA induction of SoSnRK2s in subgroup III. Co-expression network analyses indicated the existence of both conserved and variable biological functions among different SoSnRK2s members. In summary, this comprehensive analysis will facilitate further studies of the SoSnRK2 family and provide useful information for the functional validation of SoSnRK2s.
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http://dx.doi.org/10.1038/s41598-017-16152-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732291PMC
December 2017

Multifunctional Liposomes for Image-Guided Intratumoral Chemo-Phototherapy.

Adv Healthc Mater 2017 Aug 15;6(16). Epub 2017 May 15.

Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA.

Intratumoral (IT) drug injections reduce systemic toxicity, but delivered volumes and distribution can be inconsistent. To improve IT delivery paradigms, porphyrin-phospholipid (PoP) liposomes are passively loaded with three hydrophilic cargos: sulforhodamine B, a fluorophore; gadolinium-gadopentetic acid, a magnetic resonance (MR) agent; and oxaliplatin, a colorectal cancer chemotherapeutic. Liposome composition is optimized so that cargo is retained in serum and storage, but is released in less than 1 min with exposure to near infrared light. Light-triggered release occurs with PoP-induced photooxidation of unsaturated lipids and all cargos release concurrently. In subcutaneous murine colorectal tumors, drainage of released cargo is delayed when laser treatment occurs 24 h after IT injection, at doses orders of magnitude lower than systemic ones. Delayed light-triggering results in substantial tumor shrinkage relative to controls a week following treatment, although regrowth occurs subsequently. MR imaging reveals that over this time frame, pools of liposomes within the tumor migrate to adjacent regions, possibly leading to altered spatial distribution during triggered drug release. Although further characterization of cargo loading and release is required, this proof-of-principle study suggests that multimodal theranostic IT delivery approaches hold potential to both guide injections and interpret outcomes, in particular when combined with chemo-phototherapy.
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http://dx.doi.org/10.1002/adhm.201700253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568974PMC
August 2017

Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes.

Sci Rep 2016 08 11;6:31304. Epub 2016 Aug 11.

State University of New York at Buffalo, Department of Biomedical Engineering, Buffalo, New York, 14260, USA.

Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment.
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http://dx.doi.org/10.1038/srep31304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980669PMC
August 2016

Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress.

Sci Rep 2016 05 12;6:25698. Epub 2016 May 12.

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.

Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate, and severe) during the elongation stage and gene expression was analyzed using microarray technology. Physiological parameters of sugarcane showed significant alterations in response to drought stress. Based on the expression profile of 15,593 sugarcane genes, 1,501 (9.6%) genes were differentially expressed under different water-level treatments; 821 genes were upregulated and 680 genes were downregulated. A gene similarity analysis showed that approximately 62.6% of the differentially expressed genes shared homology with functional proteins. In a Gene Ontology (GO) analysis, 901 differentially expressed genes were assigned to 36 GO categories. Moreover, 325 differentially expressed genes were classified into 101 pathway categories involved in various processes, such as the biosynthesis of secondary metabolites, ribosomes, carbon metabolism, etc. In addition, some unannotated genes were detected; these may provide a basis for studies of water-deficit tolerance. The reliability of the observed expression patterns was confirmed by RT-PCR. The results of this study may help identify useful genes for improving drought tolerance in sugarcane.
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http://dx.doi.org/10.1038/srep25698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864372PMC
May 2016