Changhao Bi

Changhao Bi

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Changhao Bi

Changhao Bi

Publications by authors named "Changhao Bi"

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26Publications

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CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli.

Biotechnol J 2018 Sep 29;13(9):e1700604. Epub 2018 Jun 29.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

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September 2018

Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate.

Microb Cell Fact 2018 Jun 5;17(1):85. Epub 2018 Jun 5.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

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June 2018

Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in cell factories.

3 Biotech 2018 Jun 25;8(6):269. Epub 2018 May 25.

2Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 People's Republic of China.

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June 2018

[Improving β-carotene production in Escherichia coli by modularized regulation of the membrane synthetic pathway and morphology engineering].

Sheng Wu Gong Cheng Xue Bao 2018 May;34(5):703-711

Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

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May 2018

Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli.

Metab Eng 2017 11 31;44:13-21. Epub 2017 Aug 31.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address:

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November 2017

CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency.

Sci Rep 2017 Nov 30;7(1):16624. Epub 2017 Nov 30.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

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November 2017

Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli.

Metab Eng 2017 09 6;43(Pt A):85-91. Epub 2017 Jul 6.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China. Electronic address:

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September 2017

Improving Succinate Productivity by Engineering a Cyanobacterial CO Concentrating System (CCM) in Escherichia coli.

Biotechnol J 2017 Sep 10;12(9). Epub 2017 Aug 10.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.

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September 2017

The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway.

Metab Eng 2017 09 9;43(Pt A):37-45. Epub 2017 Aug 9.

Tianjin Institute of Industrial Biotechnology, Chinese of Academy of Sciences, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Tianjin 300308, China. Electronic address:

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September 2017

Construction of a novel anaerobic pathway in Escherichia coli for propionate production.

BMC Biotechnol 2017 04 14;17(1):38. Epub 2017 Apr 14.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

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April 2017

Type IIs restriction based combinatory modulation technique for metabolic pathway optimization.

Microb Cell Fact 2017 Mar 16;16(1):47. Epub 2017 Mar 16.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

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March 2017

A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli.

BMC Biotechnol 2017 Feb 13;17(1):10. Epub 2017 Feb 13.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.

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February 2017

Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli.

Microb Cell Fact 2016 Dec 1;15(1):202. Epub 2016 Dec 1.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

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December 2016

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9.

Microb Cell Fact 2016 Dec 1;15(1):205. Epub 2016 Dec 1.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

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December 2016

Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in .

Biotechnol Biofuels 2016 28;9:232. Epub 2016 Oct 28.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Park, Tianjin, 300308 China.

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October 2016

Engineering Corynebacterium glutamicum for violacein hyper production.

Microb Cell Fact 2016 Aug 24;15(1):148. Epub 2016 Aug 24.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

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August 2016

End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis.

J Biol Eng 2016 2;10. Epub 2016 Feb 2.

Fuels Synthesis and Technologies Divisions, Joint BioEnergy Institute, Emeryville, CA 94608 USA ; Biological Systems and Engineering Division, Lawrence Berkeley National Lab, Berkeley, CA 94720 USA ; DNA Synthesis Science Program, DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.

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February 2016

Overexpression of the Lactobacillus plantarum peptidoglycan biosynthesis murA2 gene increases the tolerance of Escherichia coli to alcohols and enhances ethanol production.

Appl Microbiol Biotechnol 2014 Oct 31;98(19):8399-411. Epub 2014 Aug 31.

Department of Chemical Engineering & Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE, 19711, USA.

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October 2014

PR-PR: cross-platform laboratory automation system.

ACS Synth Biol 2014 Aug 14;3(8):515-24. Epub 2014 Jan 14.

Fuels Synthesis Division, Joint BioEnergy Institute , Emeryville, California 94608, United States.

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August 2014

Development of a broad-host synthetic biology toolbox for Ralstonia eutropha and its application to engineering hydrocarbon biofuel production.

Microb Cell Fact 2013 Nov 13;12:107. Epub 2013 Nov 13.

Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

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November 2013

Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones.

Appl Environ Microbiol 2013 Jul 17;79(14):4433-9. Epub 2013 May 17.

Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

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July 2013

PaR-PaR laboratory automation platform.

ACS Synth Biol 2013 May 9;2(5):216-22. Epub 2012 Oct 9.

Fuels Synthesis Division, Joint BioEnergy Institute , Emeryville, California 94608, United States.

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May 2013

Functionalizing bacterial cell surfaces with a phage protein.

Chem Commun (Camb) 2013 Jan;49(9):910-2

Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

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January 2013

SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824.

J Bacteriol 2011 Oct 22;193(19):5130-7. Epub 2011 Jul 22.

Molecular Biotechnology Laboratory, Department of Chemical Engineering, Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA.

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October 2011

Genetic engineering of Enterobacter asburiae strain JDR-1 for efficient production of ethanol from hemicellulose hydrolysates.

Appl Environ Microbiol 2009 Sep 17;75(18):5743-9. Epub 2009 Jul 17.

Department of Microbiology and Cell Science, University of Florida, Bldg. 981, Museum Rd., Gainesville, FL 32611-0700, USA.

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September 2009

Complete fermentation of xylose and methylglucuronoxylose derived from methylglucuronoxylan by Enterobacter asburiae strain JDR-1.

Appl Environ Microbiol 2009 Jan 14;75(2):395-404. Epub 2008 Nov 14.

University of Florida, Department of Microbiology and Cell Science, Gainesville, FL 32611-0700, USA.

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January 2009