Publications by authors named "Chang-Lin Zhao"

15 Publications

  • Page 1 of 1

The Phylogenetic Relationship Revealed Three New Wood-Inhabiting Fungal Species From Genus .

Front Microbiol 2021 17;12:650195. Epub 2021 Mar 17.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China.

Wood-inhabiting fungi play a significant role in wood degradation and the cycle of matter in the ecological system. In the present study, three new wood-inhabiting fungal species, , , and spp. nov., are nested in , which are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer (ITS) and large subunit (nLSU) regions of the studied samples were generated, and the phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. The phylogenetic analyses inferred from ITS showed that was sister to , grouped with , and grouped with . The phylogenetic tree based on ITS + nLSU sequences demonstrated that formed a single lineage and then grouped with and . was sister to . grouped with .
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http://dx.doi.org/10.3389/fmicb.2021.650195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009992PMC
March 2021

[Moxibustion inhibits growth of tumor by down-regulating expression of FGFR1 and VEGFR2 in mice with sarcoma].

Zhen Ci Yan Jiu 2021 Feb;46(2):95-9

College of Health Science, Guangdong Pharmaceutical University, Guangzhou 510006.

Objective: To observe the effect of moxibustion on the growth of tumor and expression of fibroblast growth factor receptor 1 (FGFR1) and vascular endothelial cell growth factor receptor 2 (VEGFR2) in mice with sarcoma, so as to explore its mechanisms underlying inhibiting sarcoma growth.

Methods: C57BL/6J mice (half male and half female) were inoculated with S180 sarcoma cells to form transplanted tumors, and divided into model control, medication and moxibustion groups, with 10 mice in each group. Moxibustion was applied to the transplanted tumor directly for 10 min, once a day for 14 days. After the treatment, Luminex liquid suspension chip was used to detect the contents of serum vascular endothelial growth factor (VEGF), FGFR1 and VEGFR2. The weight of the transplanted tumor was measured, and the expression of VEGF in the transplanted tumor was detected by immunohistochemistry, and the expression of FGFR1 and VEGFR2 mRNAs in the transplanted tumor was detected by fluorescence in situ hybridization.

Results: The tumor weight, VEGF immunoactivity, serum VEGF, VEGFR2 and FGFR1 contents, and expression levels of VEGFR2 and FGFR1 mRNAs in the transplanted tumor were significantly lower in the moxibustion group than in the model group (P<0.001, P<0.01, P<0.05). Compared with the model group, the tumor weight was remarkably lower in the medication group (P<0.001). Compared with the medication group, th VEGF immunoactivity and the contents of serum VEGF, VEGFR2 and FGFR1 were significantly lower in the moxibustion group (P<0.01, P<0.05). H.E. staining showed a large number of red blood cells were observed in the microenvironment of the transplanted tumor in the moxibustion group rather than in the medication group.

Conclusion: Moxibustion can inhibit the growth of tumor in mice with sarcoma, which may be related to its function in reducing the expression of FGFR1 and VEGFR2 to inhibit angiogenesis.
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http://dx.doi.org/10.13702/j.1000-0607.200346DOI Listing
February 2021

Effects of moxibustion on Treg cells in sarcoma microenvironment.

J Integr Med 2021 Feb 13. Epub 2021 Feb 13.

Health Science College, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China. Electronic address:

Objective: To investigate the therapeutic effect of moxibustion on sarcomas from mesenchymal tissues, which have a low response rate to chemotherapy and radiotherapy.

Methods: S180 sarcoma cell line was inoculated in C57BL/6 mice to form transplanted tumor. Moxibustion therapy was directly applied at the transplanted tumor sites, at a distance of 3.0 cm, 10 min per session, till skin temperature reached 45 °C, once a day, for 14 consecutive days of intervention. After the mice were killed, serum was collected and used to detect concentrations of interleukin-10 (IL-10), transforming growth factor-β1 (TGF-β1), IL-4 and interferon-γ (IFN-γ) by Luminex liquid suspension chip. The numbers of Treg T cells and CD4CD25Forkhead Box P3 (Foxp3) T cells were detected by flow cytometry. Fluorescence in situ hybridization was used to analyze the changes of CD4, CD8, Foxp3 and TGF-β1 in the tumor microenvironment (TME).

Results: Weight of S180 transplanted tumor in the control group was (2.03 ± 0.54) g, and that in the moxibustion group was (1.27 ± 0.29) g, which was statistically different (P = 0.023). The mean value of Foxp3 T cells in the normal group was 2.01%, which increased to 3.63% after the formation of transplanted tumor, and decreased to 1.48% after moxibustion treatment. The moxibustion group also had reduced numbers of CD4CD25Foxp3 T cells in the spleen of mice with transplanted tumor. The concentrations of IL-10, TGF-β1 and IL-4 decreased in the serum of mice with transplanted tumor, while the concentration of IFN-γ increased. Moxibustion was associated with downregulation in expression of Foxp3, IL-10 and TGF-β1 genes in the transplanted tumor, and increases in the gene expression of CD4 T cells and CD8 T cells in the TME.

Conclusion: Moxibustion may have therapeutic effects on sarcomas by reducing the number of Treg cells in the blood and controlling the infiltration of Treg cells in the TME.
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http://dx.doi.org/10.1016/j.joim.2021.02.001DOI Listing
February 2021

Steccherinum tenuissimum and S. xanthum spp. nov. (Polyporales, Basidiomycota): New species from China.

PLoS One 2021 13;16(1):e0244520. Epub 2021 Jan 13.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, P. R. China.

Two new wood-inhabiting fungal species, Steccherinum tenuissimum and S. xanthum spp. nov. are described based on a combination of morphological features and molecular evidence. Steccherinum tenuissimum is characterized by an annual growth habit, resupinate basidiomata with an odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae, strongly encrusted cystidia and basidiospores measuring 3-5 × 2-3.5 μm. Steccherinum xanthum is characterized by odontioid basidiomata and a monomitic hyphal system with generative hyphae bearing clamp connections and covering by crystals, colourless, thin-walled, smooth, IKI-, CB-and has basidiospores measuring 2.7-5.5 × 1.8-4.0 μm. Sequences of the ITS and nLSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS + nLSU sequences showed that two new Steccherinum species felled into the residual polyporoid clade. Further investigation was obtained for more representative taxa in Steccherinum based on ITS + nLSU sequences, which demonstrated that S. tenuissimum and S. xanthum were sister to S. robustius with high support (100% BP, 100% BS and 1.00 BPP).
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244520PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806176PMC
January 2021

Morphological and molecular identification of four new resupinate species of (Hymenochaetales) from southern China.

MycoKeys 2020 26;65:101-118. Epub 2020 Mar 26.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China.

Four new wood-inhabiting fungal species, , , and , are proposed based on a combination of morphological and molecular evidence. is characterized by resupinate basidiomata with colliculose to tuberculate hymenial surface and broadly ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. is characterised by resupinate basidiomata with smooth, cream hymenial surface and ellipsoid, hyaline, thin-walled to slightly thick-walled basidiospores. is characterized by pruinose basidiomata with reticulate hymenial surface, presence of three kinds of cystidia and larger basidiospores (6.7-8.9 × 4.4-5.4 µm). is characterized by coriaceous basidiomata and ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. The phylogenetic analyses based on molecular data of the internal transcribed spacer (ITS) region sequences revealed that the four new species belonged to . grouped with . clade was sister to a clade comprised of . was sister to . was closely related to .
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http://dx.doi.org/10.3897/mycokeys.65.48660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125236PMC
March 2020

gen. et sp. nov. in Polyporales (Basidiomycota).

MycoKeys 2018 30(29):81-95. Epub 2018 Jan 30.

Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, P.R. China.

A new poroid wood-inhabiting fungal genus, , typified by , is proposed based on a combination of morphological features and molecular evidence. The genus is characterised by an annual growth habit, resupinate basidiocarps, becoming rigid and light-weight up on drying, a monomitic hyphal system with thick-walled generative hyphae bearing both clamp connections and simple septa, slightly amyloid, CB+ and ellipsoid, hyaline, thin-walled, smooth and IKI-, CB- basidiospores. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that belonged to the residual polyporoid clade and was closely related to . Further investigation was obtained for more representative taxa in the Meruliaceae based on ITS+nLSU sequences, in which the result demonstrated that the genus formed a monophyletic lineage with a strong support (100 % BS, 100 % BP, 1.00 BPP) and then grouped with and .
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http://dx.doi.org/10.3897/mycokeys.29.22086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804141PMC
January 2018

Dysbiosis of gut microbiota induced the disorder of helper T cells in influenza virus-infected mice.

Hum Vaccin Immunother 2015 ;11(5):1140-6

a Clinical College; Jining Medical University ; Jining , China.

It is widely understood that commensal microbiota contributes to the maintenance of intestinal homeostasis through dynamic interactions with a body's immunity. And the immune regulation is important for the influenza vaccine's effectiveness after body injection, however, the mechanism between commensal microbiota and vaccine's effectiveness remains unknown. The impact that individual bacteria species have on the balance of the systemic immune system beyond the local intestinal mucosal tissues also remains less clear, and the related mechanism is still unknown. In this study, through the administration of various antibiotics, we examined the balance of helper T cell subsets in mice after inoculating them with the influenza virus and then, attempted to imitate the clinical practice in which patients are always prescribed with an antibiotic treatment in flu season. The data indicates that the mice in each group present differential immune responses in terms of the makeup of helper T cell subsets, although the Th17 cell activity seems to not be involved in the systemic immune modulation in the mice that are susceptible to the intervention of antibiotic. Th1, Th2, and anti-inflammatory regulatory T cells have been implicated in the contribution to the systemic immune response influenced by the antibiotic-induced dysbiosis. Thus we believe that the normal intestinal flora could maintain the immune balance and inhibit the inflammatory responses, which may be useful for clinical application to take intestinal flora into consideration when influenza vaccination was used.
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http://dx.doi.org/10.1080/21645515.2015.1009805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514154PMC
February 2016

Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway.

Chin J Integr Med 2014 Jul 28;20(7):540-5. Epub 2014 Jun 28.

Department of Traditional Chinese Medicine, School of Medicine, Jinan University, Guangzhou, 510632, China.

Objective: To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1.

Methods: Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation, and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE). The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium (MTT) assay. After the virus infected cell model was established, AD was added into the medium as a treatment intervention. After 24-h co-culture, cell supernatant was collected for interferon gamma (IFN-γ) and interleukin-4 (IL-4) enzyme-linked immunosorbent assay (ELISA) detection while immunological cells for real-time polymerase chain reaction (RT-PCR).

Results: The optimal concentration of AD for anti-virus effect was 250 μg/mL. IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus, RIG-I, IFN-β promoter stimulator-1 (IPS-1), interferon regulatory factor (IRF)-7, IRF-3 and nuclear transcription factor κB (NF-κB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium, the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased, RIG-I, IPS-1, IRF-7, IRF-3 and NF-κB decreased significantly in each group with significant statistic differences (P<0.05).

Conclusions: The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality. And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.
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http://dx.doi.org/10.1007/s11655-014-1860-0DOI Listing
July 2014

Retrograde analysis of clinical characteristics of bone metastasis in 1,031 cases of preliminarily diagnosed nasopharyngeal carcinoma.

Asian Pac J Cancer Prev 2014 ;15(8):3785-8

Department of Traditional Chinese Medicine, School of Medicine, Jinan University, Guangzhou, China E-mail :

Purpose: To explore the clinical characteristics of bone metastasis (BM) in a large sample of preliminarily diagnosed nasopharyngeal carcinomas (NPCs).

Methods: The sample consisted of 1,031 patients diagnosed with NPC at first visitg clinics between October 1989 and June 2012. Several parameters including metastasis locus, T/N staging, diagnosis, therapy and prognosis of BM were analyzed retrospectively.

Results: In 70 patients who had been preliminarily diagnosed with BM, the incidence of BM in N0, N1, N2 and N3 stage was 5.7%, 17.2%, 50.2%, and 25.7%, respectively, while the incidence in T0, T1, T2 and T3 stage was 0%, 23.8%, 47.6% and 28.6% respectively. BM occurred in most common in vertebral column, rib, sternum, ilium and femur. Positive rate of Epstein-Barr virus antibody was 77.6%. The median survival time was 12 months.

Conclusion: The incidence of BM in NPC preliminarily diagnosed is about 7% and it is related to N classification but not T classification.
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http://dx.doi.org/10.7314/apjcp.2014.15.8.3785DOI Listing
January 2015

Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway.

J Asian Nat Prod Res 2012 28;14(9):877-85. Epub 2012 Aug 28.

Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China.

The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1. After immunological cells and virus-infected cells were co-cultured, quercetin and oseltamivir were also added into the medium as a treatment intervention. Then the immunological cells were collected for Real Time PCR (RT-PCR) and Western blot to determine the expression levels of genes related to TLR7 pathway. Viral infection led to cell death and increased the gene expression levels of TLR7 signal pathway. Quercetin and oseltamivir increased cell viability and reduced the expression levels of TLR7 signal pathway.
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http://dx.doi.org/10.1080/10286020.2012.702108DOI Listing
December 2012

[Effect of acupuncture on the activity of the peripheral blood T lymphocyte subsets and NK cells in patients with colorectal cancer liver metastasis].

Zhongguo Zhen Jiu 2010 Jan;30(1):10-2

Department of Oncology, Qifu Affiliated Hospital of Guangzhou TCM University, Guangzhou 511495, China.

Objective: To study the effect of acupuncture on the immune function of patients with colorectal cancer liver metastasis.

Methods: Sixty cases with colorectal cancer liver metastasis confirmed by pathology and mageology diagnosis were treated with acupuncture. Zusanli (ST 36), Sanyinjiao (SP 6), Neiguan (PC 6), Shangjuxu (ST 37), Hegu (LI 4), Taixi (KI 3), Taichong (LR 3) ,Yinlingquan (SP 9), Yanglingquan (GB 34), etc. were selected for acupuncture, and Shenque (CV 8), Guanyuan (CV 4), Qihai (CV 6), Zusanli (ST 36) were selected for moxibustion. The changes of CD(3) , CD(4) , CD(8) T cells and NK cells in value were examined with flow cytometry before and after treatment.

Results: The value of T lymphocyte subsets such as CD(3) , CD(4), and CD(8) , as well as NK cells were obviously increased after treatment, and there were significant differences between them before and after treatment.

Conclusion: Acupuncture can improve the immune function of patients with colorectal cancer liver metastasis.
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January 2010

[Association between hemoglobin scavenger receptor CD163 expression and coronary atherosclerotic severity in patients with coronary heart disease].

Zhonghua Xin Xue Guan Bing Za Zhi 2009 Jul;37(7):605-9

Department of Cardiology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.

Objective: To investigate the association between hemoglobin scavenger receptor (CD163) expression levels on monocytic surfaces and coronary atherosclerotic severity in patients with coronary heart disease (CHD) as well as the roles of CD163 in inflammation and lipidperoxidation.

Methods: Eighty-four patients were diagnosed as CHD according to the results of coronary angiography and ACC/AHA diagnostic criteria. The patients were divided into 3 groups: acute myocardial infarction (AMI) group (n = 30), unstable angina (UA) group (n = 30), stable angina (SA) group (n = 24). Another 20 patients with normal coronary artery served as control. Expression levels of CD163 on monocytes were detected by means of flow cytometry, and the results were shown as mean fluorescence intensity (mfi). All patients underwent coronary angiography and the results were further evaluated by Jenkins score. Serum CRP and LDL-C were also measured.

Results: The expression levels of CD163 on monocytes in peripheral blood were significantly higher in CHD patients compared to controls (P < 0.01) in the order of AMI group [(84.4 +/- 6.9) mfi] > UA group [(64.1 +/- 5.5) mfi, P < 0.01 vs. AMI] > SA group [(46.7 +/- 6.5) mfi, P < 0.01 vs. AMI and UA] > control group [(22.0 +/- 6.1) mfi, P < 0.01 vs. AMI, UA and SA]. The expression levels of CD163 on monocytes in patients with CHD were positively correlated with Jenkins score (r = 0.9107, P < 0.01), CRP (r = 0.766, P < 0.01) and LDL-C (r = 0.749, P < 0.01).

Conclusions: Expression levels of CD163 was significantly increased in patients with CHD and positively correlated with coronary heart disease severity and serum CRP and LDL-C.
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July 2009

[Studies on autoantigenic epitopes involving in the development of uveitis].

Zhonghua Yan Ke Za Zhi 2008 Apr;44(4):377-80

International Uveitis Study Laboratory of Guangdong Province, Uveitis Study Center of Sun Yat-sen University, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

It has been proposed that a few kinds of autoantigens imitate the development of autoimmune uveitis while the immunodominant epitopes of these antigens have not been identified. Researches on retinal S-antigen and interphotoreceptor retinoid-binding protein as well as tyrosinase-related protein epitopes mapping have shown that each autoantigen contains several immunopathogenic epitopes and immunogenic epitopes and that the immunopathogenic sites are not coincident with the immunogenic epitopes. The reactivity of peripheral blood mononuclear cells from uveitis patients against each autoantigenic epitopes displays high heterogeneity. Epitopes spreading phenomenon has been disclosed in human uveitis study and reinforced in animal experiments. Study on this epitope spreading may contribute to our understanding of immune tolerance induced by different epitopes in the treatment of autoimmune disease including uveitis.
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April 2008

Fourteen cases of neonatal ultrahyperpyrexia syndrome induced by improper care.

Adv Ther 2007 Jan-Feb;24(1):101-5

Medical Scientific College, Jinan University, Guangdong Province, China.

This report describes the clinical characteristics, treatment, and pathologic mechanisms of neonatal ultrahyperpyrexia syndrome induced by improper care. Clinical data on 14 cases of neonatal ultrahyperpyrexia syndrome induced by improper care were retrospectively analyzed. All 14 neonates had become comatose with ultrahyperpyrexia or hyperpyrexia; 8 of these patients twitched, 12 had high muscular tension, 2 exhibited low muscle tone, and 9 had respiratory dysfunction. Computed tomography revealed a patched, low dense shadow in the frontal and parietal lobes of the cerebral cortex in 9 patients; local cerebral hemorrhage occurred in 3 cases, and subarachnoid hemorrhage was reported in 2. After systematic treatment was provided, 13 neonates survived, but 1 died. Dysfunction of the neural, respiratory, and circulatory systems was the main pathologic mechanism noted in these cases of neonatal ultrahyperpyrexia syndrome induced by improper care. Early treatment, acute hypothermia, and supplemental fluids are the keys to successful therapy.
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http://dx.doi.org/10.1007/BF02849997DOI Listing
June 2007

Changes of pregnancy-associated plasma protein-A in patients with acute coronary syndrome.

Chin Med J (Engl) 2005 Nov;118(21):1827-9

Department of Cardiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

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November 2005