Publications by authors named "Chang Zhang"

257 Publications

Guided Internal Fixation Technique for Transconjunctival Orbital Fat Transposition.

J Craniofac Surg 2021 Sep 14. Epub 2021 Sep 14.

Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing, China.

Abstract: The predominant method for transconjunctival orbital fat transposition is by conducting 1 or several temporary externalized sutures. A new method that could internally and more securely fixate the fat to the periosteum remains desirable. This manuscript introduces a technique that utilizes a titanium ring to guide transconjunctival orbital fat fixation for lower blepharoplasty. With 8 to 10 mm transconjunctival incisions, the orbicularis retaining ligaments were released. An intraoperatively made ring guided the sutures that was made transcutaneously and anchored on the periosteum to exit the preseptal space through the transconjunctival incision. The orbital fat graft was then fixated internally by this suture. From October 2017 to November 2019, 51 patients were included in this study. The mean patient age was 35.7 years (ranged 28-42 years). The mean operative time for the surgical procedure was 52 minutes. No major complications were noticed in any patients. There was 1 case in which the eye bag relapses at fifth week postoperatively, but was corrected by the same approach. At the average follow-up period of 12.1 months, all patients presented significant improvement of the lower eyelid cosmesis. Forty-four patients rated the aesthetic outcomes as "Very Satisfied," the other 7 rated "Satisfied" in a self-assessment questionnaire.Global Aesthetic Improvement Scale echoed similar outcomes. This guided internal fixation technique is technically practical when properly executed. It offers a new method for facilitating orbital fat transposition in lower blepharoplasty.
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http://dx.doi.org/10.1097/SCS.0000000000008140DOI Listing
September 2021

Use of an Orbital Septum Flap for Correcting Severe Blepharoptosis.

Aesthetic Plast Surg 2021 Sep 13. Epub 2021 Sep 13.

Head and Neck Plastic and Cosmetic Surgery Center, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.

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http://dx.doi.org/10.1007/s00266-021-02570-3DOI Listing
September 2021

Microplastics influence on Hg methylation in diverse paddy soils.

J Hazard Mater 2021 Aug 12;423(Pt A):126895. Epub 2021 Aug 12.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address:

Microplastics are widespread in estuarine, coastal, and deep sea sediments. The influence of microplastics on mercury (Hg) methylation in paddy soils with different characteristics, however, has not been well reported. In this research, we conducted a microcosmic experiment using red soil and alkaline soil with 2%, 7% and 10% polyvinyl chloride microplastics (PVC-MPs). Diffusive gradients in thin film (DGT) were used to test bioavailable Hg and bioavailable methylmercury (MeHg) in soils. Results showed that PVC-MPs could decrease bioavailable MeHg concentrations both in red soil and alkaline soil. We demonstrated that these decreases could be due to three possible mechanisms: (1) PVC-MPs affected DOM composition, which resulted in a difference in combining capacity for bioavailable Hg; (2) PVC-MPs decreased MeHg via changing soil properties (including sulfate and dissolved Fe); (3) PVC-MPs affected the abundance of Proteobacteria, Firmicutes, and hgcA gene in soils. Our results emphasized the significance of investigating effects of microplastics on specific contaminants to implement effective environmental remediation strategies in polluted paddy soils.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126895DOI Listing
August 2021

MEN1 silencing aggravates tumorigenic potential of AR-independent prostate cancer cells through nuclear translocation and activation of JunD and β-catenin.

J Exp Clin Cancer Res 2021 Aug 26;40(1):270. Epub 2021 Aug 26.

Université Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon, 69008, Lyon, France.

Background: Recent studies highlighted the increased frequency of AR-low or -negative prostate cancers (PCas) and the importance of AR-independent mechanisms in driving metastatic castration-resistant PCa (mCRPC) development and progression. Several previous studies have highlighted the involvement of the MEN1 gene in PCa. In the current study, we focused on its role specifically in AR-independent PCa cells.

Methods: Cell tumorigenic features were evaluated by proliferation assay, foci formation, colony formation in soft agar, wound healing assay and xenograft experiments in mice. Quantitative RT-PCR, Western blot and immunostaining were performed to determine the expression of different factors in human PCa lines. Different ChIP-qPCR-based assays were carried out to dissect the action of JunD and β-catenin.

Results: We found that MEN1 silencing in AR-independent cell lines, DU145 and PC3, resulted in an increase in anchorage independence and cell migration, accompanied by sustained MYC expression. By searching for factors known to positively regulate MYC expression and play a relevant role in PCa development and progression, we uncovered that MEN1-KD triggered the nuclear translocation of JunD and β-catenin. ChIP and 3C analyses further demonstrated that MEN1-KD led to, on the one hand, augmented binding of JunD to the MYC 5' enhancer and increased formation of loop structure, and on the other hand, increased binding of β-catenin to the MYC promoter. Moreover, the expression of several molecular markers of EMT, including E-cadherin, BMI1, Twist1 and HIF-1α, was altered in MEN1-KD DU145 and PC3 cells. In addition, analyses using cultured cells and PC3-GFP xenografts in mice demonstrated that JunD and β-catenin are necessary for the altered tumorigenic potential triggered by MEN1 inactivation in AR-independent PCa cells. Finally, we observed a significant negative clinical correlation between MEN1 and CTNNB1 mRNA expression in primary PCa and mCRPC datasets.

Conclusions: Our current work highlights an unrecognized oncosuppressive role for menin specifically in AR-independent PCa cells, through the activation of JunD and β-catenin pathways.
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http://dx.doi.org/10.1186/s13046-021-02058-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393735PMC
August 2021

Effects of tree canopy on psychological distress: A repeated cross-sectional study before and during the COVID-19 epidemic.

Environ Res 2021 Jul 31;203:111795. Epub 2021 Jul 31.

Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; Key Laboratory of Tree Breeding and Cultivation and Urban Forest Research Centre, National Forestry and Grassland Administration, Beijing, 10091, China. Electronic address:

Background: During the COVID-19 epidemic period, people showed a stronger connection to the environment within their communities. Although tree canopy in residential areas has been shown to positively affect psychological distress, it is not clear whether the COVID-19 epidemic played a role in this process. Elucidation of the relationship between tree canopy and the impact on psychological distress during the COVID-19 epidemic could provide valuable information as to the best methods to help individuals cope with urban mental stress events.

Methods: A total of 15 randomly selected residential areas of Beijing were enrolled in this repeated cross-sectional study. A total of 900 residents were included in the two-waves of the investigation (450 residents per wave) before and during the COVID-19 epidemic (i.e., May 2019 and May 2020). Psychological distress was estimated using the 12-question General Health Questionnaire (GHQ-12). Tree canopy coverage (TCC) was measured through visual interpretation based on the 2013 data sources (World View 2 satellite imagery of Beijing urban areas with a resolution of 0.5 m). The demographic characteristics, distance to the nearest surrounding green or blue space, residential area house price, household density, and construction year were also collected in this study. A multivariate logistic regression, relative risk due to interaction (RERI), and synergy index (SI) were used to explore the relationships among tree canopy, COVID-19, and psychological distress.

Results: The negative impact of the COVID-19 epidemic on mental health was significant, with the prevalence of psychological distress increased 7.84 times (aOR = 7.84, 95% CI = 4.67-13.95) during the COVID-19 epidemic period. Tree canopy coverage in the group without psychological distress was significantly higher than that of the psychologically distressed group (31.07 ± 11.38% vs. 27.87 ± 12.97%, P = 0.005). An increase in 1% of TCC, was related to a 5% decrease in the prevalence of psychological distress (aOR = 0.95, 95% CI = 0.93-0.98). An antagonism joint action between tree canopy and the COVID-19 epidemic existed (RERI = 1.09, 95% CI = 0.72-1.47; SI = 0.16, 95% CI = 0.05-0.52), and persisted enhancing only in medium (26.45%-33.21%) and above TCC level. Correlation of GHQ items and TCC significantly differed between the COVID-19 non-epidemic and epidemic periods, with the effects of tree canopy on GHQ-12 items covering topics, such as social function and depression, presumably absent because of epidemic limitations.

Conclusions: This study indicates that the COVID-19 epidemic harmed mental health and verified the positive effects of residential tree canopy on psychological distress in Beijing. We suggest paying more attention to residents in areas of low TCC and dealing with psychological distress caused by public health stress events based on tree canopy strategies.
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http://dx.doi.org/10.1016/j.envres.2021.111795DOI Listing
July 2021

Does Psychological Capital Mediate Occupational Stress and Coping Among Nurses in ICU.

West J Nurs Res 2021 Aug 1:1939459211014426. Epub 2021 Aug 1.

School of Nursing, Peking Union Medical College, Beijing, China.

As an exploitable positive psychological state that individual performs during growth and development processes, psychological capital (PsyCap) was deemed as an essential factor in occupational health. This study aims to explore mediating effects of PsyCap on the relationship between job stress and coping style among ICU nurses. A cross-sectional sample of 288 nurses was recruited. A high-level of occupational stress (2.32 ± 0.69) and PsyCap (4.62 ± 0.93) were found among participants who mainly used a positive coping style (2.04 ± 0.59). PsyCap was significantly negatively correlated with job stress ( = -0.250;  < .01) and positively with coping style ( = 0.416;  < .01). Structural equation model analysis revealed that job stress negatively influenced PsyCap, while PsyCap positively influenced coping style. Results verified the mediating role of PsyCap and suggested that an increase in PsyCap contributes to better control of job stress and the improvement of one's coping style.
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http://dx.doi.org/10.1177/01939459211014426DOI Listing
August 2021

Recent Progress in Superhydrophilic Carbon-Based Composite Membranes for Oil/Water Emulsion Separation.

ACS Appl Mater Interfaces 2021 Aug 2;13(31):36679-36696. Epub 2021 Aug 2.

Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China.

The purification of stabilized oil/water emulsions is essential to meet the ever increasing demand for monitoring water in the environment, which has been addressed with superwetting carbon-based separation membranes. These include superhydrophilic carbon-based membranes whose progress in recent years and perspectives are reviewed in this paper. The membrane construction strategy is organized into four parts, vacuum-assisted self-assembly, sol-gel process, electrospinning, and vacuum-assisted filtration. In each section, the design strategies and their responding disadvantages have been comprehensively discussed. The challenges and prospects concerning the superhydrophilic carbon-based separation membranes for oily wastewater purification are also summarized to arouse researchers to carry out more studies.
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http://dx.doi.org/10.1021/acsami.1c07737DOI Listing
August 2021

Length of Cervical Stenosis, Admission ASIA Motor Scores, and BASIC Scores are Predictors of Recovery Rate Following Central Cord Syndrome.

Spine (Phila Pa 1976) 2021 Jul 23. Epub 2021 Jul 23.

Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong and Department of Radiology, Queen Mary Hospital, Hong Kong.

Study Design: A retrospective cohort study.

Objective: This study aims to determine whether quantitative MRI parameters and radiological scoring systems could be used as a reliable assessment tool for predicting neurological recovery trajectory following acute traumatic central cord injury syndrome (CCS).

Summary Of Background Data: Controversy remains in whether CCS should be managed conservatively or by early surgical decompression. It is essential to understand how clinical and radiological parameters correlate with neurological deficits and how they predict recovery trajectories.

Methods: We identified patients with CCS admitted between 2011 - 2018 with a minimum of 1-year follow-up. Cervical MRIs were analysed for cord / canal dimensions, BASIC scores and sagittal grading as ordinal scales of intraparenchymal cord injury. JOA recovery rates (≥50% as good, < 50% as poor) were analysed against these variables by logistic regression and receiver operator characteristic (ROC) curves. Additionally, we evaluated ASIA motor scale (AMS) scores / recovery rates.

Results: 60 patients were included, of which 30 were managed conservatively and 30 via surgical decompression. The average follow-up duration for the entire cohort was (51.1 ± 25.7) months. Upon admission, sagittal grading correlated with AMS and JOA scores (p < 0.01, β = 0.48). Volume of the C2 - C7 canal and axial cord area over the site of maximal compression correlated with AMS and JOA scores respectively (p = 0.04, β = 0.26; p = 0.01, β = 0.28). We determined admission AMS > 61 to be a clinical cutoff for good recovery (AUC = 0.74, 95% CI: 0.61-0.85, sensitivity 80.9%, specificity 69.2%, p < 0.01). Radiological cutoffs to identify patients with poor recovery rates were length of cervical spinal stenosis > 3.9 cm (AUC = 0.76, 95%CI: 0.63-0.87, specificity 91.7%, sensitivity 52.2%, p < 0.01), and BASIC score > 1 (AUC = 0.69, 95%CI: 0.56-0.81, specificity 80.5%, sensitivity 51.1%, p = 0.02). Surgical decompression performed as a salvage procedure upon plateau of recovery did not improve neurological outcomes.

Conclusion: Clinical and radiological parameters upon presentation were prognosticative of neurological recovery rates in CCS. Surgery performed beyond the acute post-injury period failed to improve outcomes.Level of Evidence: 3.
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http://dx.doi.org/10.1097/BRS.0000000000004178DOI Listing
July 2021

Do perfluoroalkyl substances aggravate the occurrence of obesity-associated glucolipid metabolic disease?

Environ Res 2021 Jul 19;202:111724. Epub 2021 Jul 19.

College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, China.

Background: Since 2016, more and more studies have been conducted to explore the combination of obesity and perfluoroalkyl substances (PFASs) exposure, and the results indicate that PFASs may be connected with the occurrence of obesity-associated glucolipid metabolic disease (GLMD).

Objectives: This article summarizes the epidemiological studies on PFASs and obesity-related GLMD, as well as relevant experimental evidence.

Results: (i) Both obesity and PFASs exposure can cause disorder of glucose and lipid metabolism (GLM). (ii) Obesity is a pivotal factor in the high incidence of GLMD induce by PFASs. (iii) PFASs are aggravating the occurrence of obesity-associated GLMD [e.g., diabetes, cardiovascular disease (CVD), and liver disease].

Conclusion: The paper fills the gaps among environmental chemistry/epidemiology/toxicology area research. More importantly, PFASs should be taken into account to explain the high-prevalence of obesity-related GLMD.

Future Direction: Three research programs are proposed to explore the synergistic mechanism of PFASs and obesity. In addition, three suggestions are recommended to solve the harm of PFASs pollutants to human beings.
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http://dx.doi.org/10.1016/j.envres.2021.111724DOI Listing
July 2021

Chromium-Catalyzed Allylic Defluorinative Ketyl Olefin Coupling.

J Am Chem Soc 2021 Aug 22;143(30):11602-11610. Epub 2021 Jul 22.

Hefei National Laboratory for Physical Science at the Microscale and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

Herein we report a chromium-catalyzed allylic defluorinative ketyl olefin coupling between aldehydes and α-trifluoromethyl alkenes, which provides novel and efficient access to diverse -difluorohomoallylic alcohols. Remarkably, the high chemoselectivity of this reaction enables the conversion of the formyl moiety in the presence of various easily reducible functionalities including ketone, organohalides, aziridine, sulfone, alkyne, and unactivated alkene. The utility of this method is demonstrated by various simple derivatizations of the attached hydroxyl group of the coupling products. The preliminary mechanistic investigations suggest a reaction pathway with a rate-limiting C-C forming step followed by facile β-fluoro elimination.
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http://dx.doi.org/10.1021/jacs.1c04531DOI Listing
August 2021

The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress.

BMC Genomics 2021 Jul 18;22(1):549. Epub 2021 Jul 18.

Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China.

Background: In plants, Calcium (Ca) acts as a universal messenger in various signal transduction pathways, including responses to biotic and abiotic stresses and regulation of cellular and developmental processes. The Ca/cation antiporter (CaCA) superfamily proteins play vital roles in the transport of Ca and/or other cations. However, the characteristics of these superfamily members in Saccharum and their evolutionary and functional implications have remained unclear.

Results: A total of 34 CaCA genes in Saccharum spontaneum, 5 CaCA genes in Saccharum spp. R570, and 14 CaCA genes in Sorghum bicolor were identified and characterized. These genes consisted of the H/cation exchanger (CAX), cation/Ca exchanger (CCX), EF-hand / CAX (EFCAX), and Mg/H exchanger (MHX) families, among which the CCX and EFCAX could be classified into three groups while the CAX could be divided into two groups. The exon/intron structures and motif compositions suggested that the members in the same group were highly conserved. Synteny analysis of CaCAs established their orthologous and paralogous relationships among the superfamily in S. spontaneum, R570, and S. bicolor. The results of protein-protein interactions indicated that these CaCA proteins had direct or indirect interactions. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis demonstrated that most members of Saccharum CaCA genes exhibited a similar expression pattern in response to hormonal (abscisic acid, ABA) treatment but played various roles in response to biotic (Sporisorium scitamineum) and abiotic (cold) stresses. Furthermore, ScCAX4, a gene encoding a cytoplasm, plasma membrane and nucleus positioning protein, was isolated from sugarcane. This gene was constitutively expressed in different sugarcane tissues and its expression was only induced at 3 and 6 h time points after ABA treatment, however was inhibited and indued in the whole process under cold and S. scitamineum stresses, respectively.

Conclusions: This study systematically conducted comparative analyses of CaCA superfamily genes among S. spontaneum, R570, and S. bicolor, delineating their sequence and structure characteristics, classification, evolutionary history, and putative functions. These results not only provided rich gene resources for exploring the molecular mechanism of the CaCA superfamily genes but also offered guidance and reference for research on other gene families in Saccharum.
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http://dx.doi.org/10.1186/s12864-021-07828-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286586PMC
July 2021

Multi-omics landscape of circadian rhythm pathway alterations in Glioma.

Bioengineered 2021 Dec;12(1):3294-3308

Department of Internal Medicine, Guang Dong Second Hospital of Traditional Chinese Medicine, Guangzhou, China.

Circadian rhythm pathway was demonstrated pathological functions in glioma on single-gene level. We aim to depict the multi-omics landscape of circadian rhythm pathway alteration in glioma using bioinformatic analyses. Multi-omics data were obtained from "cBioPortal" database. Comparisons were done regarding clinical parameters, differential-expressed genes and functional annotations. A pathway index was generated using the expression data from TCGA and GTEx to quantify the general alteration level of the pathway with clinical association of circadian rhythm pathway index explored. A total of 30 genes were mapped on the circadian rhythm pathway. Genomic profile ofcircadian rhythm pathway genes exhibited distinct characteristics on multiple levels between lower grade glioma (LGG) and glioblastoma multiforme (GBM) patients. LGG patients presented significantly higher frequencies of multi-omics mutations, as well as significant clinical relevance, on single-gene level. Differential-expressed genes between LGG and GBM patients revealed different functions between subtypes that related to the alteration of circadian rhythm pathway. LGG have significantly higher pathway index than normal brain tissue, while GBM significantly lower than normal tissue (P < 0.01), indicating distinctly altered circadian pathway in LGG. Circadian rhythm pathway index correlated with the prognosis of LGG, but not GBM, patients, with higher score indicating better survival outcome (LGG: HR = 0.39, 95% CI: 0.26 - 0.59, P < 0.001). In conclusion, LGG have more multi-omics alterations of circadian rhythm pathway than GBM. Quantification of circadian rhythm pathway using pathway index demonstrated hyperactivated pathway status in LGG and correlated with the prognosis of LGG patients.
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http://dx.doi.org/10.1080/21655979.2021.1947075DOI Listing
December 2021

First-line Daratumumab in Addition to Chemotherapy for Newly Diagnosed Multiple Myeloma Patients Who are Transplant Ineligible: A Cost-Effectiveness Analysis.

Clin Ther 2021 Jun 27. Epub 2021 Jun 27.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Purpose: Daratumumab is a standard-of-care treatment for newly diagnosed multiple myeloma (NDMM). According to the ALCYONE trial, the addition of daratumumab to bortezomib, melphalan, and prednisone (D-VMP) provides significantly longer overall survival and progression-free survival than bortezomib, melphalan, and prednisone (VMP) in patients with NDMM. However, considering the high price of daratumumab, it is necessary to conduct further research on its efficacy and cost. This study evaluated the cost-effectiveness, from the US payer perspective, of D-VMP vs VMP in the first-line setting for patients with NDMM who are not eligible for autologous stem cell transplantation.

Methods: A Markov model was developed to estimate the lifetime cost and effectiveness of VMP with or without daratumumab as the first-line therapy for patients with NDMM. Univariable sensitivity analysis and probabilistic sensitivity analysis were performed to address the model robustness and uncertainty. Expected value of perfect information analysis was conducted to explore the uncertainty of decision-making and future costs.

Findings: D-VMP provides an additional 2.417 quality-adjusted life years (QALYs), at a cost of $30,893 per QALY. Sensitivity analysis revealed that the transition probability of progression-free survival in D-VMP strategy, the price of daratumumab, and body weight of the patient influenced the model results most strongly. Probabilistic sensitivity analysis showed that D-VMP versus VMP has a 90.8% probability of being cost-effective at the $150,000/QALY willingness-to-pay (WTP) threshold. The population expected value of perfect information was $2150 million at a WTP threshold of $50,000/QALY and $1481 million at $100,000/QALY.

Implications: In this study, D-VMP was estimated to be cost-effective compared with VMP for patients with NDMM at a WTP threshold of $150,000/QALY. (Clin Ther. 2021;43:XXX-XXX) © 2021 Elsevier HS Journals, Inc.
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http://dx.doi.org/10.1016/j.clinthera.2021.05.015DOI Listing
June 2021

C/[email protected]@C with Triple Balances for Superior Microwave Absorption Performance.

ACS Appl Mater Interfaces 2021 Jul 29;13(27):32037-32045. Epub 2021 Jun 29.

Laboratory of Advanced Materials, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, P. R. China.

It is very promising and challenging to construct a yolk-shell structure with highly efficient microwave absorption (MA) performance through a simple fabrication process. Here, a novel C/[email protected]@C (MCC) yolk-shell structure has been successfully synthesized by one-step calcination without additional processing. The as-obtained MCC composites with tunable crystallinity degrees and hollowness can be obtained by treatment at various temperatures. The MCC composites treated at 700 °C (MCC-700) show an impressive MA performance, and the optimal reflection loss of -53.2 dB and an effective absorption bandwidth of 5.4 GHz can be obtained. This excellent performance results from multiple balance mechanisms. First, the regulated permittivity of MCC-700 due to proper crystallinity and hollowness is beneficial for the balance between dielectric loss (tan δ) and impedance match (). Second, the optimal balance between the increasing polarization range and decreasing polarization intensity can be achieved, which is favorable for the improvement of the MA performance. Third, the multicore yolk-shell structure of MCC-700 is conducive to multiple scattering and continuous energy dissipation. Thus, our new findings provide a rational way for the utilization of yolk-shell structural manganese-based materials.
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http://dx.doi.org/10.1021/acsami.1c08555DOI Listing
July 2021

Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.

PLoS One 2021 25;16(6):e0253188. Epub 2021 Jun 25.

College of Bioengineering, Henan University of Technology, Zhengzhou High-technology Zero, Henan Province, 450001, China.

ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medicinal plant. Here, we scanned R. glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R. glutinosa.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253188PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232422PMC
June 2021

Self-assembled micro-flowers of ultrathin Au/BiOCOOH nanosheets photocatalytic degradation of tetracycline hydrochloride and reduction of CO.

Chemosphere 2021 Nov 15;283:131228. Epub 2021 Jun 15.

College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, China. Electronic address:

The low separation efficiency of carriers and weak light response of photocatalysts severely limit the application of photocatalysis technology. Herein, we prepared a visible light responsive self-assembled micro-flowers of ultrathin bismuth oxide formate nanosheets supported by gold nanoparticles (Au/BiOCOOH) composite photocatalyst via hydrothermal method. The physicochemical and photoelectric properties of obtained-photocatalysts were completely analyzed via a range of characterization means. Compared with bare BiOCOOH, the photocatalytic activity of Au/BiOCOOH was significantly improved. 2.0%Au/BiOCOOH possessed the highest rate constant of 0.0054 min for degradation of tetracycline hydrochloride (TC-HCl), which was nearly 13.5 times higher than that of BiOCOOH. The intermediate products were analyzed by 3D EEM and HPLC/MS, and the antibacterial ability of intermediate products with 2.0%Au/BiOCOOH significantly descended. In order to explore the potential of practical applications, photocatalytic experiments were also implemented through different water sources and solar light irradiation. Furthermore, the photocatalytic activity was also investigated by photocatalytic reduction of carbon dioxide (CO). The excellent photocatalytic activity owed to the enhanced separation of charge carriers and light absorption ability by the surface plasmon resonance (SPR) effect of Au nanoparticles. The work may provide a feasible strategy to obtain efficient BiOCOOH-based photocatalyst.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131228DOI Listing
November 2021

LncRNA DRAIC promotes apoptosis and inhibits proliferation of colorectal cancer via regulating MiR-223.

Minerva Med 2021 Jun 18. Epub 2021 Jun 18.

Department of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China -

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http://dx.doi.org/10.23736/S0026-4806.21.07605-9DOI Listing
June 2021

JianpiQinghua granule reduced PPI dosage in patients with nonerosive reflux disease: A multicenter, randomized, double-blind, double-dummy, noninferiority study.

Phytomedicine 2021 Jul 3;88:153584. Epub 2021 May 3.

China Academy of Chinese Medical Sciences, Beijing, China. Electronic address:

Background: Proton pump inhibitors (PPIs) play an important role in the treatment of nonerosive reflux disease (NERD), but their long-term and excessive uses have been associated with safety concerns. Chinese herbal medicine (CHM) has become a popular alternative treatment for this condition.

Methods: A total of 204 patients were randomly assigned to the combination group or PPI group (1:1 ratio). They were given JianpiQinghua (JQ) granules (34.8 g) plus omeprazole (10 mg) plus dummy omeprazole (10 mg) or dummy JQ granules (34.8 g) plus omeprazole (20 mg) daily for 4 weeks. The primary endpoints were the rate of sufficient relief and complete resolution of GERD Q at week 4. Metabonomics and the gut microbiota were also assessed.

Results: Complete resolution was observed in 40.8% of patients in the combination group and 26.8% of patients in the PPI group after 4 weeks (FAS analysis, OR, 1.88; 95% CI, 1.03-3.44; p = 0.039). Sufficient relief was observed in 50% of patients in the combination group and 43.30% of patients in the PPI group after 4 weeks (FAS analysis, OR, 1.31; 95% CI, 0.74-2.30; p = 0.35). Three patients had liver dysfunction, one of whom had a mild case and 2 of whom had moderate-to-severe cases in the combination group. Patients in the combination group showed a significant increase in richness and diversity of their gut microbiota compared with those in the PPI group. Metabonomics showed that the combination therapy could correct the glutamate metabolism pathway.

Conclusion: Our findings demonstrate the superior efficacy of JQ granules combined with omeprazole (10 mg) vs. omeprazole (20 mg) in terms of symptom relief in patients with NERD.

Trial Registration: ClinicalTrials.gov number NCT02892357. Registered on 14 February 2019.
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http://dx.doi.org/10.1016/j.phymed.2021.153584DOI Listing
July 2021

Development and implementation of a standardized training program for newly graduated mental health nurses: Process and preliminary outcomes.

Nurse Educ Today 2021 Sep 12;104:104953. Epub 2021 May 12.

School of Nursing, Peking Union Medical College, No. 33, Badachu Road, Shijingshan District, Beijing 100144, China.

Background: Training programs are crucial for newly graduated nurses transitioning from nursing school to independent clinical nursing careers. However, few studies have focused on the training of newly graduated mental health nurses.

Objectives: The aim of this study was to develop, implement, and preliminarily evaluate a standardized training program for newly graduated nurses entering psychiatric nursing.

Design: The Delphi technique was adopted for program development, and a quasi-experimental design was employed for program implementation.

Settings: Six mental health institutions were involved in the practice training, and among these, three university-affiliated psychiatric hospitals were the study sites for theoretical training.

Participants: A total of 180 newly graduated nurses hired by the six hospitals were involved and 154 finished the training program.

Methods: The program was developed using the Delphi method based on the results of a literature review and semi-structured interview with clinical practitioners. Change in mental health nurses' core competencies before and after the program was compared during program implementation. Focus group interviews were employed to collect the opinions and influence of the standardized training program after the intervention, and content analysis was used to analyze it.

Results: A two-round Delphi expert consultation was done, and 7 core competency elements and 37 training topics were designed for the standardized training program. The six-month training program consisted of two months for course training and four months of practical training, which were guided by a manual. Total CIRN scale score improved (142.29 ± 34.87 vs. 160.35 ± 32.46, P < 0.01) after receiving the program as did the scores for each dimension (P < 0.01). Participants gave positive feedback and suggested optimal training length and certification for the program.

Conclusions: The standardized training program focused on mental health nursing proved to be a viable method of novice nurse training and resulted in positive outcomes. The program length and its long-term effects deserve further exploration to determine the best practical training program for sustaining optimal development of mental health nursing.
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http://dx.doi.org/10.1016/j.nedt.2021.104953DOI Listing
September 2021

A Polarization Boosted Strategy for the Modification of Transition Metal Dichalcogenides as Electrocatalysts for Water-Splitting.

Small 2021 Jul 3;17(26):e2100510. Epub 2021 Jun 3.

Laboratory of Advanced Materials, Department of Materials Science and Collaborative Innovation Center of Chemistry for Energy Materials (iChem), Fudan University, Shanghai, 200438, P. R. China.

The design and fabrication of transition metal dichalcogenides (TMDs) are of paramount significance for water-splitting process. However, the limited active sites and restricted conductivity prevent their further application. Herein, a polarization boosted strategy is put forward for the modification of TMDs to promote the absorption of the intermediates, leading to the improved catalytic performance. By the forced assembly of TMDs (WS as the example) and carbon nanotubes (CNTs) via spray-drying method, such frameworks can remarkably achieve low overpotentials and superior durability in alkaline media, which is superior to most of the TMDs-based catalysts. The two-electrode cell for water-splitting also exhibits perfect activity and stability. The enhanced catalytic performance of WS /CNTs composite is mainly owing to the strong polarized coupling between CNTs and WS nanosheets, which significantly promotes the charge redistribution on the interface of CNTs and WS . Density functional theory (DFT) calculations show that the CNTs enrich the electron content of WS , which favors electron transportation and accelerates the catalysis. Moreover, the size of WS is restricted caused by the confinement of CNTs, leading to the increased numbers of active sites, further improving the catalysis. This work opens a feasible route to achieve the optimized assembling of TMDs and CNTs for efficient water-splitting process.
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http://dx.doi.org/10.1002/smll.202100510DOI Listing
July 2021

Effects of Vertical Lifting Distance on Upper-Body Muscle Fatigue.

Int J Environ Res Public Health 2021 05 20;18(10). Epub 2021 May 20.

Key Laboratory of Advanced Manufacturing Technology of the Ministry of Education, Guizhou University, Guiyang 550025, China.

Manual material handling (MMH) is commonly demanded in the manufacturing industry. Occupational muscle fatigue of the arm, shoulder, and back, which arise from MMH tasks, can cause work absences and low efficiency. The available literature presents the lack of the fatigue comparison between targeted muscles, on the same part or on different parts. The main aim of the present study was to evaluate and compare the fatigue of upper-body muscles during repetitive bending tasks, an experiment involving 12 male subjects has been conducted to simulate material handling during furniture board drilling. The vertical lifting distance was chosen to be the single independent variable, and the three levels were 0, 250, and 500 mm. Surface electromyography (sEMG) was used to measure the muscle fatigue of the biceps brachii, upper trapezius, and multifidus, while the sEMG parameters, including the normalized electromyographic amplitude (Normalized EA) and mean power frequency (MPF), of the target muscles were analyzed. The experimental results reveal that during the manual handling tasks, the biceps brachii was the most relaxed muscle, contributing the least muscle tension, while the multifidus was the most easily fatigued muscle. Furthermore, the EMG MPF fatigue threshold (MPFFT) of multifidus muscle tension was tested to estimate its maximum workload in the long-term muscle contraction. In conclusion, bending angle should be maintained to a small range or bending should even be avoided during material-handling tasks.
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http://dx.doi.org/10.3390/ijerph18105468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160884PMC
May 2021

Guided suturing technique for midface lift through minimal temporal incision.

J Plast Reconstr Aesthet Surg 2021 Apr 8. Epub 2021 Apr 8.

Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Science, 33 Badachu Rd., Shijingshan District, Beijing 100144, China. Electronic address:

Background: The midface has been a difficult zone to manage in rejuvenating surgeries. The major challenge for midface lift is to achieve extensive dissection and elevate the composite tissues en bloc through minimal incisions.

Methods: In a total of 22 composite midface lift cases, a titanium cable wire was used. The wire was made into an elastic loop that guided three SMAS-suspending sutures out of the subcutaneous plane through a short incision at the temporal region. Then, each suture was fixated to the deep temporal fascia. Postoperative complications and the subjective assessment of the patients were evaluated.

Results: Surgeries were successfully commenced in all cases. Midface sagging, eye bags, and nasolabial folds all exhibited considerable improvements. Major complications such as nerve injury and alopecia were not observed. The patients were followed up for an average of 16.9 months. In general, 90% of the patients were satisfied with their outcome.

Conclusion: This guided suspension suturing technique effectuates a more convenient and efficient way to conduct multi-points anchorage of the composite malar flap. When properly executed, this technique allows the anchorage sutures to bite into the deeper layer of the SMAS that ensures firm suspensions in midface rejuvenation surgery.
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http://dx.doi.org/10.1016/j.bjps.2021.03.061DOI Listing
April 2021

A cross-tissue transcriptome-wide association study identifies novel susceptibility genes for lung cancer in Chinese populations.

Hum Mol Genet 2021 Aug;30(17):1666-1676

Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China.

Although dozens of susceptibility loci have been identified for lung cancer in genome-wide association studies (GWASs), the susceptibility genes and underlying mechanisms remain unclear. In this study, we conducted a cross-tissue transcriptome-wide association study (TWAS) with UTMOST based on summary statistics from 13 327 lung cancer cases and 13 328 controls and the genetic-expression matrix over 44 human tissues in the Genotype-Tissue Expression (GTEx) project. After further evaluating the associations in each tissue, we revealed 6 susceptibility genes in known loci and identified 12 novel ones. Among those, five novel genes, including DCAF16 (Pcross-tissue = 2.57 × 10-5, PLung = 2.89 × 10-5), CBL (Pcross-tissue = 5.08 × 10-7, PLung = 1.82 × 10-4), ATR (Pcross-tissue = 1.45 × 10-5, PLung = 9.68 × 10-5), GYPE (Pcross-tissue = 1.45 × 10-5, PLung = 2.17 × 10-3) and PARD3 (Pcross-tissue = 5.79 × 10-6, PLung = 4.05 × 10-3), were significantly associated with the risk of lung cancer in both cross-tissue and lung tissue models. Further colocalization analysis indicated that rs7667864 (C > A) and rs2298650 (G > T) drove the GWAS association signals at 4p15.31-32 (OR = 1.09, 95%CI: 1.04-1.12, PGWAS = 5.54 × 10-5) and 11q23.3 (OR = 1.08, 95%CI: 1.04-1.13, PGWAS = 5.55 × 10-5), as well as the expression of DCAF16 (βGTEx = 0.24, PGTEx = 9.81 × 10-15; βNJLCC = 0.29, PNJLCC = 3.84 × 10-8) and CBL (βGTEx = -0.17, PGTEx = 2.82 × 10-8; βNJLCC = -0.32, PNJLCC = 2.61 × 10-7) in lung tissue. Functional annotations and phenotype assays supported the carcinogenic effect of these novel susceptibility genes in lung carcinogenesis.
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http://dx.doi.org/10.1093/hmg/ddab119DOI Listing
August 2021

Insight the roles of loosely-bound and tightly-bound extracellular polymeric substances on Cu, Zn and Pb biosorption process with Desulfovibrio vulgaris.

J Colloid Interface Sci 2021 Aug 29;596:408-419. Epub 2021 Mar 29.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.

The aim of this study is to explore the fate and mechanism of metal cations of biosorption in the Desulfovibrio vulgaris system (including bacterial cells and secreted loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound extracellular polymeric substances (TB-EPS)). The relative contribution of EPS (TB-EPS and LB-EPS) to the adsorption of three metal cations is much greater than that of bacterial cells, and the adsorption capacity of Pb on EPS (TB-EPS and LB-EPS) is much greater than that of Cu and Zn (Pb > Cu > Zn). The order of absorption capacity was as follows: LB-EPS > TB-EPS > bacterial cells, the adsorption contribution of EPS (including TB-EPS and LB-EPS) to Cu, Zn and Pb accounted for total adsorption capacity was 82%, 83% and 86%, respectively. It was suggested that LB-EPS was the first reaction barrier of immobilization metal cations before metal cations was able to pass through EPS and react with cells. The adsorption process was dominated by complexation and electrostatic interaction. The three-dimensional excitation-emission matrix (3D-EEM) identified two main fluorescence peaks of the aromatic and tryptophan protein-like substances in EPS. According to the synchronous fluorescence spectra, the tryptophan protein-like substances were gradually quenched with increased metal cations concentrations, which the quencher mechanism is dynamic quenching. The findings of this work are significant to reveal the fate of Cu, Zn and Pb during its sorption process onto Desulfovibrio vulgaris, and provide useful information of the interaction between Desulfovibrio vulgaris and its secreted EPS with metal cations.
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http://dx.doi.org/10.1016/j.jcis.2021.03.152DOI Listing
August 2021

Silencing XIST mitigated lipopolysaccharide (LPS)-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and TLR4/NF-κB signaling pathway.

Open Life Sci 2021 6;16(1):108-127. Epub 2021 Feb 6.

Department of Pediatrics, Guangdong Second Hospital of Traditional Chinese Medicine, No. 60 Hengfu Road, 510000, Guangzhou, Guangdong, China.

Background: Emerging evidence shows that long noncoding RNA (lncRNA) has been a novel insight in various diseases, including pneumonia. Even though lncRNA X-inactive-specific transcript (XIST) is well studied, its role in pneumonia remains to be largely unrevealed.

Methods: Expression of XIST, miRNA-30b-5p (miR-30b-5p), and CC chemokine ligand 16 (CCL16) was detected using reverse transcriptase quantitative polymerase chain reaction and western blotting; their interaction was confirmed by dual-luciferase reporter assay. Apoptosis, inflammation, and toll-like receptor 4 (TLR4)/NF-κB signaling pathway were measured using methyl thiazolyl tetrazolium assay, flow cytometry, western blotting, and enzyme-linked immunosorbent assay.

Results: Lipopolysaccharide (LPS) stimulation decreased cell viability and B cell lymphoma (Bcl)-2 expression, and increased cell apoptosis rate and expression of Bcl-2-associated X protein (Bax), cleaved-caspase-3, interleukin (IL)-6, IL-1β, and tumor necrosis factor α (TNF-α) in WI-38 cells. Expression of XIST and CCL16 was upregulated in the serum of patients with pneumonia and LPS-induced WI-38 cells, respectively; silencing XIST and CCL16 could suppress LPS-induced apoptosis and inflammation in WI-38 cells, and this protection was abolished by miR-30b-5p downregulation. Moreover, XIST and CCL16 could physically bind to miR-30b-5p, and XIST regulated CCL16 expression via sponging miR-30b-5p. TLR4 and phosphorylated P65 (p-P65) and p-IκB-α were highly induced by LPS treatment, and this upregulation was diminished by blocking XIST, accompanied with CCL16 downregulation and miR-30b-5p upregulation.

Conclusions: Silencing XIST could alleviate LPS-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and inhibiting TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1515/biol-2021-0005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988359PMC
February 2021

Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells.

Mol Ther Methods Clin Dev 2021 Jun 3;21:107-120. Epub 2021 Mar 3.

Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.

Non-viral platforms can be applied rapidly and cost-effectively for chimeric antigen receptor (CAR)-T cell manufacturing. In the present paper, we describe in detail a clinically relevant manufacturing process for NKG2D CAR-T cells through electroporation of CAR-encoding piggyBac transposon plasmids and expansion with K562 artificial antigen-presenting cells. With an optimized protocol, we generated the final cell therapy products with 89.2% ± 10.2% NKG2D CAR-positive cells and achieved the corresponding antigen-dependent expansion between 50,000 and 60,000 folds within 4 weeks. To facilitate repeated CAR-T cell infusions, we evaluated the practicality of cryopreservation followed by post-thaw expansion and an extended manufacturing process for up to 9 rounds of weekly K562 cell stimulation. We found that neither compromised the anti-tumor activity of NKG2D CAR-T cells. Interestingly, the expression of T cell exhaustion markers TIGIT, TIM3, and LAG3 was reduced with extended manufacturing. To enhance the safety profile of the NKG2D CAR-T cells, we incorporated a full-length CD20 transgene in tandem with the CAR construct and demonstrated that autologous NK cells could mediate efficient antibody-dependent cell-mediated cytotoxicity to remove these CAR-T cells. Collectively, our study illustrates a protocol that generates large numbers of efficacious NKG2D CAR-T cells suitable for multiple rounds of infusions.
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http://dx.doi.org/10.1016/j.omtm.2021.02.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005737PMC
June 2021

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders.

Mol Med Rep 2021 05 24;23(5). Epub 2021 Mar 24.

Department of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, P.R. China.

Intestinal inflammation frequently occurs alongside dysmotility, which is characterized by altered myosin light chain phosphorylation levels. Curcumin, an active component from the ginger family, is reported to confer anti‑inflammatory effects. However, the effects of curcumin on both diarrhea and constipation associated inflammation remains to be elucidated. The present study was designed to investigate the effects of curcumin on diarrhea and constipation and to determine the related mechanisms. Sprague‑Dawley rats were used to establish diarrhea and constipation models via intracolonic acetic acid (4%) instillation or cold water gavage for 2 weeks, respectively. Blood samples were collected to measure the serum levels of the cytokines TNF‑α and IL‑1β using ELISA kits. Western blotting was performed to measure NF‑κB, RhoA, Rho‑related kinase 2, phosphorylated MLC, phosphorylated myosin phosphorylated target subunit 1, 130k Da‑MLC kinase (MLCK), c‑kit tyrosine kinase protein expression, and reverse transcription‑quantitative PCR was conducted to measure MLCK expression levels. The results indicated that curcumin reversed the elevations in the pro‑inflammatory cytokines IL‑1β and TNF‑α by inhibiting the NF‑κB pathway in rats with diarrhea and constipation. The results also indicated that myosin light chain (MLC) phosphorylation in intestinal smooth muscle was positively and negatively associated with the motility of inflammation‑related diarrhea and constipation in rats, respectively. Curcumin significantly reversed the increased MLC phosphorylation in the jejunum of the rats with diarrhea, significantly enhanced the reductions in inflammatory mediators, including TNF‑α and IL‑1β, of rats with constipation and significantly ameliorated the related hyper‑motility and hypo‑motility in rats with both diarrhea and constipation. In conclusion, the potential roles of the MLC kinase, c‑kit tyrosine and Rho A/Rho‑associated kinase 2 pathways, which are involved in curcumin‑induced amelioration of inflammation‑related diarrhea and constipation, were explored in the present study. Results from the present study suggested that curcumin has potential therapeutic value for treating intestinal inflammation and inflammation‑related motility disorders.
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http://dx.doi.org/10.3892/mmr.2021.12030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008224PMC
May 2021

Wide field of view handheld smart fundus camera for telemedicine applications.

J Med Imaging (Bellingham) 2021 Mar 16;8(2):026001. Epub 2021 Mar 16.

University of Miami, Department of Electrical and Computer Engineering, Coral Gables, Florida, United States.

We report that a large field-of-view (FOV) retinal image can be acquired by a smart fundus camera. This handheld system consists of a Raspberry Pi board, a touch screen display, a customized optical lens group, a ring light-emitting diode, and a Li-battery. Wide FOV of is observed with proper lens configuration and can be expanded using image stitching algorithms. This customized handheld fundus camera provides better image quality than cellphone-based fundus imaging solutions and offers more operational features than traditional portable fundus cameras. It may benefit field-portable ophthalmic diagnostic applications.
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http://dx.doi.org/10.1117/1.JMI.8.2.026001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961555PMC
March 2021

The long noncoding RNA MALAT1 modulates adipose loss in cancer-associated cachexia by suppressing adipogenesis through PPAR-γ.

Nutr Metab (Lond) 2021 Mar 10;18(1):27. Epub 2021 Mar 10.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Background: Cancer-associated cachexia is a multifactorial syndrome defined by progressive weight loss with ongoing loss of adipose tissue and skeletal muscle. Adipose loss occurs in the early stage of cachexia and is associated with reduced quality of life and survival time. Although numerous lncRNAs are regarded as novel regulators in adipose metabolism, the role of lncRNAs that selectively modulate the development of adipose loss in cachexia remains limited.

Methods: In this study, we analyzed microarray data of lncRNAs in adipose loss and further explored the function and mechanism of MALAT1 in adipose loss. First, we explored the expression and function of MALAT1 in adipose cell by quantitative PCR and RNA knockdown. Subsequently, the mechanism of MALAT1 involvement in adipose loss was analyzed via RNA-seq, bioinformatics analysis and reporter gene assay. Finally, we explored the clinical significance of MALAT1 through correlation analysis.

Results: Cellular experiments revealed that knocking down MALAT1 significantly inhibited the process of adipogenesis. RNA-seq data showed that numerous adipogenic genes were downregulated upon MALAT1 knockdown. A protein-protein interaction network analysis identified PPAR-γ as the central node transcription factor, the inhibition of which explains the downregulation of numerous adipogenic genes. A reporter gene assay suggested that MALAT1 can regulate the gene expression of PPAR-γ at the transcriptional level. Moreover, MALAT1 was weakly expressed in the subcutaneous white adipose tissue of cancer-associated cachexia patients and was related to low fat mass index and poor prognosis in cancer patients.

Conclusions: This study indicated that MALAT1 is associated with adipose loss in cancer-associated cachexia by regulating adipogenesis through PPAR-γ, which may potentially be a novel target for the diagnosis and treatment of cancer-associated cachexia in the clinic.
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http://dx.doi.org/10.1186/s12986-021-00557-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944636PMC
March 2021

Genome-wide identification, characterization and expression analysis of the carotenoid cleavage oxygenase (CCO) gene family in Saccharum.

Plant Physiol Biochem 2021 May 3;162:196-210. Epub 2021 Mar 3.

Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China; Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. Electronic address:

Carotenoid cleavage oxygenases (CCOs) play crucial roles in plant growth and development, as well as in the response to phytohormonal, biotic and abiotic stresses. However, comprehensive and systematic research on the CCO gene family has not yet been conducted in Saccharum. In this study, 47 SsCCO and 14 ShCCO genes were identified and characterized in Saccharum spontaneum and Saccharum spp. R570 cultivar, respectively. The SsCCOs consisted of 38 SsCCDs and 9 SsNCEDs, while ShCCOs contained 11 ShCCDs and 3 ShNCEDs. The SsCCO family could be divided into 7 groups, while ShCCO family into 5 groups. The genes/proteins contained similar compositions within the same group, and the evolutionary mechanisms differed between S. spontaneum and R570. Gene Ontology annotation implied that CCOs were involved in many physiological and biochemical processes. Additionally, 41 SsCCOs were regulated by 19 miRNA families, and 8 ShCCOs by 9 miRNA families. Cis-regulatory elements analysis suggested that CCO genes functioned in the process of growth and development or under the phytohormonal, biotic and abiotic stresses. qRT-PCR analysis indicated that nine CCO genes from different groups exhibited similar expression patterns under abscisic acid treatment, while more divergent profiles were observed in response to Sporisorium scitamineum and cold stresses. Herein, comparative genomics analysis of the CCO gene family between S. spontaneum and R570 was conducted to investigate its evolution and functions. This is the first report on the CCO gene family in S. spontaneum and R570, thus providing valuable information and facilitating further investigation into its function in the future.
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http://dx.doi.org/10.1016/j.plaphy.2021.02.041DOI Listing
May 2021
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