Publications by authors named "Chang Wang"

539 Publications

Src homolog and collagen homolog1 isoforms in acute and chronic liver injuries.

Life Sci 2021 Mar 1;273:119302. Epub 2021 Mar 1.

Department of Hepatology, The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address:

Src homolog and collagen homolog (SHC) proteins are adaptor proteins bound to cell surface receptors that play an important role in signal transduction and related diseases. As an important member of the SHC protein family, SHC1 regulates cell proliferation and apoptosis, reactive oxygen species (ROS) production, and oxidative stress. Three isomeric proteins namely, p46shc, p52shc, and p66shc, are produced from the same SHC1 gene locus. All the three proteins are found in the liver, and are widely expressed in various hepatic cells. SHC1 has been proven to be associated with acute and chronic liver injuries of different etiologies, and plays important roles in liver fibrosis and hepatocellular carcinoma (HCC). Therefore, this review summarizes recent studies that discuss and explore the role of SHC1 in the occurrence and progression of liver diseases. We also provide a theoretical basis for future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119302DOI Listing
March 2021

Force Decoding of Caudal Forelimb Area and Rostral Forelimb Area in Chronic Stroke Rats.

IEEE Trans Biomed Eng 2021 Mar 4;PP. Epub 2021 Mar 4.

Brain machine interfaces (BMIs) used for movement restoration primarily rely on studies of motor decoding. It has been proved that local field potentials (LFPs) from primary motor cortex and premotor cortex of normal rodents could be used for decoding motor signals. However, few studies have explored the decoding performance of these brain areas under motor cortex damage. In this work, we focus on force decoding performance of LFPs spectrum from both ipsilesional caudal forelimb area (CFA) and rostral forelimb area (RFA) of rodents with ischemia over CFA. After three months of ischemia induced by photothrombosis over CFA, the power of high-frequency (>120Hz) bands from both of CFA and RFA can decode force signals by Kalman filters. The fair performance of CFA indicates motor reorganization over penumbra. Further exploration of RFA decoding ability proves that at least four electrodes of RFA should be used on decoding and those electrodes far from CFA of stroke rats could achieve almost as good results as close to CFA of normal rats, which indicates the motor remapping. Experimental results show the long-term PM LFPs decoding performance of stroke rats as trained Kalman model could be used to accurately decode force some days later which provides a possibility for online decoding system. In conclusion, our work shows that even under CFA ischemia, power from high-frequency LFPs of RFA is still able to accurately decode force signals and has long stability, which provides the possibility of BMIs for motor function reconstruction of chronic stroke patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TBME.2021.3063903DOI Listing
March 2021

Microbial signatures in the lower airways of mechanically ventilated COVID19 patients associated with poor clinical outcome.

medRxiv 2021 Feb 26. Epub 2021 Feb 26.

Mortality among patients with COVID-19 and respiratory failure is high and there are no known lower airway biomarkers that predict clinical outcome. We investigated whether bacterial respiratory infections and viral load were associated with poor clinical outcome and host immune tone. We obtained bacterial and fungal culture data from 589 critically ill subjects with COVID-19 requiring mechanical ventilation. On a subset of the subjects that underwent bronchoscopy, we also quantified SARS-CoV-2 viral load, analyzed the microbiome of the lower airways by metagenome and metatranscriptome analyses and profiled the host immune response. We found that isolation of a hospital-acquired respiratory pathogen was not associated with fatal outcome. However, poor clinical outcome was associated with enrichment of the lower airway microbiota with an oral commensal ( ), while high SARS-CoV-2 viral burden, poor anti-SARS-CoV-2 antibody response, together with a unique host transcriptome profile of the lower airways were most predictive of mortality. Collectively, these data support the hypothesis that 1) the extent of viral infectivity drives mortality in severe COVID-19, and therefore 2) clinical management strategies targeting viral replication and host responses to SARS-CoV-2 should be prioritized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1101/2021.02.23.21252221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924286PMC
February 2021

The NCI Genomic Data Commons.

Nat Genet 2021 Feb 22. Epub 2021 Feb 22.

University of Chicago, Center for Translational Data Science, Chicago, IL, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41588-021-00791-5DOI Listing
February 2021

Androgen Plays a Carcinogenic Role in EOC via the PI3K/AKT Signaling Pathway in an AR-Dependent Manner.

J Cancer 2021 21;12(6):1815-1825. Epub 2021 Jan 21.

Department of Anatomy, Hebei Medical University, Shijiazhuang, China.

Epithelial ovarian cancer (EOC) is one of the most common gynecological cancers with the highest mortality rate. Studies indicate that androgens contribute to initiation or progression of EOC through poorly understood mechanisms, however, in the phase II clinical studies of antiandrogen therapy for EOC, neither flutamide nor bicalutamide showed good antitumor effects. Based on the contradictions, the purpose of this study was to explore the role of androgen receptor (AR) in the androgen pathogenesis of EOC and the possible mechanism, and further to find an indicator to screen the anti-androgen therapy sensitive cases. In this study, 70 EOC biopsies and 17 para-cancerous tissues with complete medical information were collected and analyzed. The expression of the androgen receptor (AR) was detected by immunohistochemistry. In addition, ovarian cancer cell lines were used for studies to further explore the role of androgen in cell proliferation and the possible mechanisms. The results showed that the expression of AR in ovarian cancer tissues was significantly elevated compared to the para-cancerous tissues, particularly in low-grade EOC, and the presence of high AR expression often suggested a worse prognosis. The study indicated that testosterone promoted the proliferation of the AR-positive SKOV3 cell line, which could be blocked by flutamide, but not in the AR-negative A2780 cell line. Next, we showed that testosterone-promoted proliferation in SKOV3 cells was abolished after we knocked out the AR. The mechanism studies revealed that the p-AKT expression in the ovarian cancer tissue was increased compared to the para-cancerous tissues, following a pattern similar to the increase of AR expression. Furthermore, the deletion and overexpression of SKOV3 cells' ARs lead to corresponding changes in the p-AKT levels. In addition, the BEZ235, an inhibitor of the PI3K/AKT signaling pathway blocked the proliferative effect of testosterone in SKOV3 cells. We showed that testosterone was able to promote the proliferation of ovarian cancer cells through activating the PI3K/AKT signaling pathway in an AR dependent manner and AR may be a screening indicator for anti-androgen therapy sensitive cases of EOC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.51099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890324PMC
January 2021

Reliability of EEG microstate analysis at different electrode densities during propofol-induced transitions of brain states.

Neuroimage 2021 Feb 13;231:117861. Epub 2021 Feb 13.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; National Engineering Research Center for Healthcare Devices, Guangzhou 510500, China; The Key Laboratory of Neuro-informatics and Rehabilitation Engineering of Ministry of Civil Affairs, Xi'an 710049, China. Electronic address:

Electroencephalogram (EEG) microstate analysis is a promising and effective spatio-temporal method that can segment signals into several quasi-stable classes, providing a great opportunity to investigate short-range and long-range neural dynamics. However, there are still many controversies in terms of reproducibility and reliability when selecting different parameters or datatypes. In this study, five electrode configurations (91, 64, 32, 19, and 8 channels) were used to measure the reliability of microstate analysis at different electrode densities during propofol-induced sedation. First, the microstate topography and parameters at five different electrode densities were compared in the baseline (BS) condition and the moderate sedation (MD) condition, respectively. The intraclass correlation coefficient (ICC) and coefficient of variation (CV) were introduced to quantify the consistency of the microstate parameters. Second, statistical analysis and classification between BS and MD were performed to determine whether the microstate differences between different conditions remained stable at different electrode densities, and ICC was also calculated between the different conditions to measure the consistency of the results in a single condition. The results showed that in both the BS or MD condition, respectively, there were few significant differences in the microstate parameters among the 91-, 64-, and 32-channel configurations, with most of the differences observed between the 19- or 8-channel configurations and the other configurations. The ICC and CV data also showed that the consistency among the 91-, 64-, and 32-channel configurations was better than that among all five electrode configurations after including the 19- and 8-channel configurations. Furthermore, the significant differences between the conditions in the 91-channel configuration remained stable at the 64- and 32-channel resolutions, but disappeared at the 19- and 8-channel resolutions. In addition, the classification and ICC results showed that the microstate analysis became unreliable with fewer than 20 electrodes. The findings of this study support the hypothesis that microstate analysis of different brain states is more reliable with higher electrode densities; the use of a small number of channels is not recommended.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroimage.2021.117861DOI Listing
February 2021

Strong Terahertz Absorption of Monolayer Graphene Embedded into a Microcavity.

Nanomaterials (Basel) 2021 Feb 7;11(2). Epub 2021 Feb 7.

School of Physics, University of Wollongong, Wollongong, NSW 2522, Australia.

Terahertz reflection behaviors of metallic-grating-dielectric-metal (MGDM) microcavity with a monolayer graphene embedded into the dielectric layer are theoretically investigated. A tunable wideband reflection dip at about the Fabry-Pérot resonant frequency of the structure is found. The reflectance at the dip frequency can be electrically tuned in the range of 96.5% and 8.8%. Because of the subwavelength distance between the metallic grating and the monolayer graphene, both of the evanescent grating slit waveguide modes and the evanescent Rayleigh modes play key roles in the strong absorption by the graphene layer. The dependence of reflection behaviors on the carrier scattering rate of graphene is analyzed. A prototype MGDM-graphene structure is fabricated to verify the theoretical analysis. Our investigations are helpful for the developments of electrically controlled terahertz modulators, switches, and reconfigurable antennas based on the MGDM-graphene structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11020421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915544PMC
February 2021

Recent advances in the Rh-catalyzed cascade arene C-H bond activation/annulation toward diverse heterocyclic compounds.

Org Biomol Chem 2021 Mar;19(8):1705-1721

Institute of New Materials & Industry Technology, College of Chemistry & Materials Engineering, Wenzhou University, Wenzhou 325035, P. R. China.

The Rh-catalyzed C-H bond activation/annulation provides a new strategy for the synthesis of new frameworks. In this review, we summarize the recent research on the Rh-catalyzed cascade arene C-H bond activation/annulation toward diverse heterocyclic compounds. The application, scope, limitations and mechanism of these transformations are also discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0ob02377aDOI Listing
March 2021

Contamination, distribution, and risk assessment of antibiotics in the urban surface water of the Pearl River in Guangzhou, South China.

Environ Monit Assess 2021 Jan 28;193(2):98. Epub 2021 Jan 28.

Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Provincial Engineering Research Center for Online Monitoring of Water Pollution, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, China.

To assess the impact of antibiotic pollution to the ecosystem in urban water, the occurrence, seasonal, and spatial distributions, potential sources, and ecological risks of 18 targeted antibiotics in urban river, Pearl River located in Guangzhou city, were investigated. Surface water samples were sampled from 24 sites in Guangzhou center of Pearl River during dry and wet seasons. The results indicated that the concentrations of antibiotic residues were at the nanogram per liter level, except sulfamethazine (SMD) (μg/L). Sulfonamides (SAs) were the dominant antibiotics, contributing 60.4-65.0% to the total antibiotics. The concentrations of SAs, fluoroquinolones (QUs), macrolides (MLs), tetracyclines (TCs), and lincosamides (LCs) were higher in dry season than those in wet season at most sampling sites, which possibly resulted from the dilution effect of heavy rainfall. The concentrations of the antibiotic residues in Guangzhou were comparable or higher than other urban rivers. The calculation on risk quotients indicated that erythromycin-HO (ETM-HO) and tetracycline (TC) were of high risks. The source identification by the Pearson correlation analysis and principal component analysis-multiple linear regression (PCA-MLR) method suggested that municipal wastewater treatment plants were primary sources of antibiotics. These results would provide important information for the environmental protect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10661-021-08887-5DOI Listing
January 2021

Inhibition of ROS/NLRP3/Caspase-1 mediated pyroptosis attenuates cadmium-induced apoptosis in duck renal tubular epithelial cells.

Environ Pollut 2020 Oct 23;273:115919. Epub 2020 Oct 23.

Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, PR China. Electronic address:

Cadmium (Cd) is an occupational and environmental pollutant, which mainly causes nephrotoxicity by damaging renal proximal tubular cells. To evaluate the effects of Cd on pyroptosis and the relationship between pyroptosis and apoptosis in duck renal tubular epithelial cells, the cells were cultured with 3CdSO·8HO (0, 2.5, 5.0, or 10.0 μM Cd), N-acetyl-L-cysteine (NAC) (100.0 μM), Z-YVAD-FMK (10.0 μM) or the combination of Cd and NAC or Z-YVAD-FMK for 12 h, and then cytotoxicity was assessed. The results evidenced that Cd significantly increased the releases of interleukin-18 (IL-18) and interleukin-1β (IL-1β), lactate dehydrogenase (LDH) and nitric oxide (NO), relative conductivity and cellular reactive oxygen species (ROS) level. Simultaneously, Cd also markedly upregulated NLRP3, Caspase-1, ASC, NEK7, IL-1β and IL-18 mRNA levels and NLRP3, Caspase-1 p20, GSDMD and ASC protein levels. Additionally, NAC notably improved the changes of above indicators induced by Cd. Combined treatment with Cd and Z-YVAD-FMK remarkably elevated Bcl-2 mRNA and protein levels, inhibited p53, Bax, Bak-1, Cyt C, Caspase-9 and Caspase-3 mRNA levels and p53, Bax, Bak-1, Caspase-9/cleaved Caspase-9 and Caspase-3/cleaved Caspase-3 protein levels, increased mitochondrial membrane potential (MMP), decreased apoptosis ratio and cell damage compared to treatment with Cd alone. Taken together, Cd exposure induces duck renal tubular epithelial cell pyroptosis through ROS/NLRP3/Caspase-1 signaling pathway, and inhibiting Caspase-1 dependent pyroptosis attenuates Cd-induced apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.115919DOI Listing
October 2020

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.

Aging (Albany NY) 2021 Jan 20;13(3):4370-4387. Epub 2021 Jan 20.

Cell Therapy Center, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250013, China.

Transplantation of mesenchymal stromal cells (MSCs) improves functional recovery in experimental models of spinal cord injury (SCI), but the mechanism is not fully understood. Activation of procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), a collagen-modifying enzyme, reportedly follows MSC transplantation in an SCI animal model. We investigated the regulation of PLOD2 expression and its potential contribution to the neuroprotective effects of adipose-derived stromal cells (ADSCs) following mechanical injury to neurons and SCI . ADSCs enhanced wound healing and promoted functional recovery after their implantation near injury sites in a rat SCI model. These effects correlated with upregulation of PLOD2, MAP2, NSE and GAP43, and downregulation of GFAP, which is indicative of improved neuronal survival and axonal regeneration as well as reduced glial scar formation. The neurorestorative effect of ADSCs was weakened after inhibition of PLOD2 expression. ADSCs appeared to induce PLOD2 upregulation via TGF-β1 secretion, as ADSC-mediated PLOD2 expression, neuronal survival, and functional recovery after SCI were largely prevented by SB431542, a TGF-(1 receptor inhibitor. These findings indicate that ADSCs reduce lesion size and promote functional recovery after SCI mainly through activation of a TGF-β1/P-Samd3/PLOD2 pathway in spinal cord neurons.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906172PMC
January 2021

Using the Atherogenic Index of Plasma to Estimate the Prevalence of Ischemic Stroke within a General Population in a Rural Area of China.

Biomed Res Int 2020 14;2020:7197054. Epub 2020 Dec 14.

Department of Cardiovascular Medicine, The First Hospital of China Medical University, Shenyang, Liaoning 110001, China.

Objective: To investigate the relationship between the atherogenic index of plasma (AIP) and ischemic stroke.

Design: We collected a range of data from 11,495 residents (aged ≥35 years; 54.28% female) residing in rural areas of northeast China between January and August 2013, including fasting lipid profile and anthropometric parameters. Logistic regression models were used to evaluate the correlation between AIP and ischemic stroke. Category-free analysis was used to determine whether AIP enhanced our capacity to estimate ischemic stroke.

Results: Irrespective of gender, AIP was independently associated with the occurrence of ischemic stroke. The prevalence of ischemic stroke increased significantly from the lowest quartile to the highest quartile (females: 10.5%-48.7%, < 0.001; males: 22.0%-36.5%, = 0.08). After adjusting for age, gender, income, education, smoking, drinking, exercise, hypertension, body mass index (BMI), diabetes, atrial fibrillation, and a family history of stroke, we found that for every 1 standard deviation (SD) increase in AIP, there was a 34.8% and 20.9% increase in the prevalence of stroke for females and males, respectively. Curve fitting was also used to evaluate the linear relationship between AIP and the occurrence of ischemic stroke. Category-free analysis indicated that AIP significantly enhanced our ability to estimate ischemic stroke in both females (NRI (95% confidence interval (CI)): 0.188 (0.105-0.270)) and males (NRI (95% CI): 0.175 (0.017-0.333)).

Conclusion: Analyses detected a significant and positive linear relationship between AIP and the prevalence of ischemic stroke. This relationship was independent of a range of conventional risk factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/7197054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787721PMC
December 2020

Is There any Additional Benefit of Multiple Doses of Tocilizumab in COVID-19 Patients?

Cureus 2020 Dec 22;12(12):e12214. Epub 2020 Dec 22.

Internal Medicine, Monmouth Medical Center, Long Branch, USA.

Many patients with coronavirus disease 2019 (COVID-19) have a hyperactive immune response (cytokine storm) which has been incriminated in multiorgan dysfunction (MOD). Interleukin-6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are the key cytokines involved in mediating systemic inflammation and triggering endothelial dysfunction. To limit these effects, IL-6 receptor inhibitors (IL6ri) have been used in COVID-19 patients. The best approach regarding the total number of doses in COVID-19 patients is still unclear. In this single-center retrospective study, we investigated if multiple doses of tocilizumab (TCZ) prevented deterioration of COVID-19 patients. Patients were divided into two cohorts based on the number of TCZ doses; cohort 1 (received one dose) and cohort 2 (received ≥ two doses). In both cohorts, all-cause-mortality was the primary outcome. Of 270 hospitalized patients with COVID-19, 81 patients received TCZ. Fifty patients received one dose of TCZ and 31 received ≥ two doses. All-cause-mortality in cohort 2 remained higher (41.9%) suggesting that there was no additional benefit of multiple doses of TCZ to prevent the primary outcome. In addition, multiple doses of TCZ did not change any other secondary outcome [(ICU admission, acute kidney injury (AKI), acute respiratory distress syndrome (ARDS), acute cardiac injury (ACI), thrombotic events, septic shock, and total hospital stay].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7759/cureus.12214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815303PMC
December 2020

MDCT-Based Radiomics Features for the Differentiation of Serous Borderline Ovarian Tumors and Serous Malignant Ovarian Tumors.

Cancer Manag Res 2021 12;13:329-336. Epub 2021 Jan 12.

Department of Radiology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, People's Republic of China.

Objective: To investigate whether multidetector computed tomography (MDCT)-based radiomics features can discriminate between serous borderline ovarian tumors (SBOTs) and serous malignant ovarian tumors (SMOTs).

Patients And Methods: Eighty patients with SBOTs and 102 patients with SMOTs, confirmed by pathology (training set: n = 127; validation set: n = 55) from December 2017 to June 2020, were enrolled in this study. The interclass correlation coefficient (ICC) and least absolute shrinkage and selection operator (LASSO) regression were applied to select radiomics parameters derived from MDCT images on the arterial phase (AP), venous phase (VP), and equilibrium phase (EP). Receiver operating characteristic (ROC) analysis of each selected parameter was carried out. Heat maps were created to illustrate the distribution of the radiomics parameters. Three models incorporating selected radiomics parameters generated by support vector machine (SVM) classifiers in each phase were analyzed by ROC and compared using the DeLong test.

Results: The most predictive features selected by ICC and LASSO regression between SBOTs and SMOTs included 9 radiomics parameters on AP, VP, and EP each. Three models on AP, VP, and EP incorporating the selected features generated by SVM classifiers produced AUCs of 0.80 (accuracy, 0.75; sensitivity, 0.74; specificity, 0.75), 0.86 (accuracy, 0.78; sensitivity, 0.80; specificity, 0.75), and 0.73 (accuracy, 0.69; sensitivity, 0.71; specificity, 0.67), respectively. There were no significant differences in the AUCs among the three models (AP vs. VP, P = 0.199; AP vs. EP, P = 0.260; VP vs. EP, P = 0.793).

Conclusion: MDCT-based radiomics features could be used as biomarkers for the differentiation of SBOTs and SMOTs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S284220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814232PMC
January 2021

METTL3 restrains papillary thyroid cancer progression via mA/c-Rel/IL-8-mediated neutrophil infiltration.

Mol Ther 2021 Jan 21. Epub 2021 Jan 21.

Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Growing evidence indicates that N6-methyladenosine (mA) is the most pervasive RNA modification in eukaryotic cells. However, the specific role of METTL3 in papillary thyroid carcinoma (PTC) initiation and development remains elusive. Here we found that downregulation of METTL3 was correlated with malignant progression and poor prognosis in PTC. A variety of gain- and loss-of-function studies clarified the effect of METTL3 on regulation of growth and metastasis of PTC cells in vitro and in vivo. By combining RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation sequencing (meRIP-seq), our mechanistic studies pinpointed c-Rel and RelA as downstream mA targets of METTL3. Disruption of METTL3 elicited secretion of interleukin-8 (IL-8), and elevated concentrations of IL-8 promoted recruitment of tumor-associated neutrophils (TANs) in chemotaxis assays and mouse models. Administration of the IL-8 antagonist SB225002 substantially retarded tumor growth and abolished TAN accumulation in immunodeficient mice. Our findings revealed that METTL3 played a pivotal tumor-suppressor role in PTC carcinogenesis through c-Rel and RelA inactivation of the nuclear factor κB (NF-κB) pathway by cooperating with YTHDF2 and altered TAN infiltration to regulate tumor growth, which extends our understanding of the relationship between mA modification and plasticity of the tumor microenvironment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymthe.2021.01.019DOI Listing
January 2021

Silver-Catalyzed Radical Cascade Sulfonation/Cycloaddition for the Construction of Multifunctional Succimides Containing Separable /-Isomers.

J Org Chem 2021 Feb 21;86(3):2173-2183. Epub 2021 Jan 21.

Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People's Republic of China.

A silver-catalyzed cascade cycloaddition of -1,6-enynes, affording multifunctional succimide frameworks initiated by the arylsulfonyl radical addition, has been developed. This process shows mild reaction conditions, excellent structural selectivity, and broad functional group tolerance. In addition, the /-isomers can be easily separated, which provides an efficient method for obtaining pure /-configuration products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.0c02275DOI Listing
February 2021

Efficacy of Trimetazidine in Limiting Periprocedural Myocardial Injury in Patients Undergoing Percutaneous Coronary Intervention: A Systematic Review and Meta-Analysis.

Angiology 2021 Jan 21:3319720987745. Epub 2021 Jan 21.

Department of Cardiology, 74569China-Japan Union Hospital of Jilin University, Changchun, Jilin, P. R. China.

We systematically searched the literature to assess the efficacy of trimetazidine in reducing periprocedural myocardial injury and improving postoperative left ventricular ejection fraction (LVEF) in patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI). An electronic search was conducted based on the PubMed, Ovid, Scopus, Springer, CENTRAL, and Google Scholar databases; 14 randomized controlled trials (RCTs) were included. Our meta-analysis showed a significant reduction in cardiac troponin I (cTnI) levels with trimetazidine compared with controls ( < .00001) but not in serum creatine kinase-myocardial band levels ( = .49). There were significantly reduced odds of ischemic ST-T segment changes with trimetazidine ( = .0.03) but lack of significant difference in the incidence of anginal attacks between the 2 groups ( = .10). Results also suggest significantly higher LVEF with trimetazidine compared with controls ( < .00001). Meta-regression analysis indicated no influence of duration of trimetazidine therapy on cTnI levels. The administration of preprocedure trimetazidine may have a role in reducing periprocedural myocardial injury in patients with CAD undergoing PCI. Evidence also suggests that postoperative trimetazidine may improve LVEF in the short term. Lack of high-quality trials and the heterogeneity of studies limit the ability of our analysis to draw strong conclusions. Further well-designed RCTs are required to supplement current evidence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0003319720987745DOI Listing
January 2021

Aberrant SLC6A14 Expression Promotes Proliferation and Metastasis of Colorectal Cancer via Enhancing the JAK2/STAT3 Pathway.

Onco Targets Ther 2021 13;14:379-392. Epub 2021 Jan 13.

Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Background: Solute carrier family 6 member 14 (SLC6A14) is a high-capacity amino acid transporter in mammalian cells. It has gained increasing attention for its potential involvement in the progression and metabolic reprogramming of various malignant tumors. However, the role of SLC6A14 in colorectal cancer (CRC) remains unclear.

Methods: Real-time polymerase chain reaction (qRT-PCR), immunoblotting and immunohistochemistry were carried out to detect the expression level of SLC6A14 in human CRC tissues and CRC-derived cell lines. HCT-116 and Caco-2 cell lines were selected to conduct in vitro functional studies. Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, cell migration and invasion assays were performed to investigate the role of SLC6A14 in CRC cells. Besides, azoxymethane/dextran sulfate sodium salt (AOM/DSS)-induced CRC and tumor xenograft models were constructed to explore the effects of SLC6A14 blockade or overexpression during tumor progression in vivo.

Results: SLC6A14 was substantially increased in human CRC samples and higher levels of SLC6A14 was correlated with advanced tumor stage, lymph node metastasis and dismal survival of CRC patients. SLC6A14 markedly promoted cell growth, inhibited cell apoptosis, and exacerbated migration and invasion of CRC cells in vitro. Mechanistically, SLC6A14 aggravated these malignant phenotypes through activating JAK2/STAT3 signaling pathway, and inhibiting JAK2/STAT3 signaling with specific inhibitors could reverse SLC6A14-mediated tumorigenic effects. Besides, two different animal studies verified the tumor-promoting effect of SLC6A14 in CRC.

Conclusion: Our data illustrated the crucial function of SLC6A14 during CRC progression, suggesting SLC6A14/JAK2/STAT3 axis may serve as novel therapeutic targets for patients with CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S288709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812055PMC
January 2021

Nutritional features-based clustering analysis as a feasible approach for early identification of malnutrition in patients with cancer.

Eur J Clin Nutr 2021 Jan 18. Epub 2021 Jan 18.

Department of Clinical Nutrition, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China.

Background: Malnutrition is prevalent that can impair multiple clinical outcomes in oncology populations. This study aimed to develop and utilize a tool to optimize the early identification of malnutrition in patients with cancer.

Methods: We performed an observational cohort study including 3998 patients with cancer at two teaching hospitals in China. Hierarchical clustering was performed to classify the patients into well-nourished or malnourished clusters based on 17 features reflecting the phenotypic and etiologic dimensions of malnutrition. Associations between the identified clusters and patient characteristics were analyzed. A nomogram for predicting the malnutrition probability was constructed and independent validation was performed to explore its clinical significance.

Results: The cluster analysis identified a well-nourished cluster (n = 2736, 68.4%) and a malnourished cluster (n = 1262, 31.6%) in the study population, which showed significant agreement with the Patient-Generated Subjective Global Assessment and the Global Leadership Initiative on Malnutrition criteria (both P < 0.001). The malnourished cluster was negatively associated with the nutritional status, physical status, quality of life, short-term outcomes and was an independent risk factor for survival (HR = 1.38, 95%CI = 1.22-1.55, P < 0.001). Sex, gastrointestinal symptoms, weight loss percentages (within and beyond 6 months), calf circumference, and body mass index were incorporated to develop the nomogram, which showed high performance to predict malnutrition (AUC = 0.972, 95%CI = 0.960-0.983). The decision curve analysis and independent external validation further demonstrated the effectiveness and clinical usefulness of the tool.

Conclusions: Nutritional features-based clustering analysis is a feasible approach to define malnutrition. The derived nomogram shows effectiveness for the early identification of malnutrition in patients with cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41430-020-00844-8DOI Listing
January 2021

Development and validation of a rapid-decision pathway to diagnose malnutrition in patients with lung cancer.

Nutrition 2020 Dec 5;84:111102. Epub 2020 Dec 5.

Department of Clinical Nutrition, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, China. Electronic address:

Objectives: Malnutrition is frequently developed and outcome-related in patients with lung cancer (LC). Making a rapid and accurate diagnosis of malnutrition is the major concern for dietitians and clinicians.

Methods: We performed a multicenter, observational cohort study including 1219 patients with LC. Malnutrition was diagnosed using the Global Leadership Initiative on Malnutrition criteria, and the study population was randomly divided into a training group (n = 914) and a validation group (n = 305). A nomogram (to diagnose malnutrition) and two decision trees (to diagnose and grade malnutrition, respectively) were independently developed and tested. A random forest algorithm was used to calculate relative variable importance.

Results: The Global Leadership Initiative on Malnutrition criteria identified 292 patients with malnutrition (24%). Sex, body mass index, weight loss within 6 mo, weight loss beyond 6 mo, calf circumference, and handgrip strength to weight ratio were screened for model development. The nomogram showed good discrimination with an area under the curve (AUC) of 0.982 (95% confidence interval, 0.969-0.995) and good calibration in the validation group. A decision curve analysis demonstrated that the nomogram was clinically useful. The diagnostic tree showed an accuracy of 0.98 (Kappa = 0.942; AUC = 0.978; 95% confidence interval, 0.964-0.992), and the classification tree showed an accuracy of 0.98 (Kappa = 0.955; AUC = 0.987) in the validation group. Weight loss within 6 mo contributed the largest importance to both trees.

Conclusions: This study presents a rapid-decision pathway, including a set of tools that can be conveniently used to facilitate the diagnosis and severity grading of malnutrition in patients with LC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nut.2020.111102DOI Listing
December 2020

Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis.

Cell Death Dis 2021 Jan 15;12(1):86. Epub 2021 Jan 15.

Department of Dermatology, the Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, Changsha, 410005, Hunan, China.

Psoriasis is a chronic inflammatory disease of the skin with highly complex pathogenesis. In this study, we identified lncRNA SPRR2C (small proline-rich protein 2C) as a hub gene with a critical effect on the pathogenesis of psoriasis and response to treatment using both weighted gene coexpression network analysis (WGCNA) and differential expression analysis. SPRR2C expression was significantly upregulated in both psoriatic lesion samples and HaCaT cell lines in response to IL-22 treatment. After SPRR2C knockdown, IL-22-induced suppression of HaCaT proliferation, changes in the KRT5/14/1/10 protein levels, and suppression of the IL-1β, IL-6, and TNF-α mRNA levels were dramatically reversed. In the coexpression network with SPRR2C based on GSE114286, miR-330 was significantly negatively correlated with SPRR2C, while STAT1 and S100A7 were positively correlated with SPRR2C. By binding to miR-330, SPRR2C competed with STAT1 and S100A7 to counteract miR-330-mediated suppression of STAT1 and S100A7. MiR-330 overexpression also reversed the IL-22-induced changes in HaCaT cell lines; in response to IL-22 treatment, miR-330 inhibition significantly attenuated the effects of SPRR2C knockdown. STAT1 and S100A7 expression was significantly upregulated in psoriatic lesion samples. The expression of miR-330 had a negative correlation with the expression of SPRR2C, while the expression of SPRR2C had a positive correlation with the expression of STAT1 and S100A7. Thus, SPRR2C modulates the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. WGCNA might uncover additional biological pathways that are crucial in the pathogenesis and response to the treatment of psoriasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-020-03305-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810847PMC
January 2021

LncRNA FAM83A-AS1 promotes ESCC progression by regulating miR-214/CDC25B axis.

J Cancer 2021 1;12(4):1200-1211. Epub 2021 Jan 1.

Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.

Recent researches have pinpointed that long non-coding RNA (lncRNA) was tightly related to the carcinogenesis. However, the function of lncRNA in esophageal cell squamous carcinoma (ESCC) remains to be explored. In the current study, we assessed the expression pattern and the biological function of FAM83A-AS1 in ESCC. qRT-PCR was used to detect the expression of FAM83A-AS1, miR-214, and CDC25B expression in ESCC tissues and cell lines. CCK-8, transwell, apoptosis and cell cycle assays were performed to define the function of FAM83A-AS1 in ESCC cells. Furthermore, the regulation of miR-214 by FAM83A-AS1 was defined by qRT- PCR and rescue assays. In addition, the association between CDC25B, miR-214, CDC25B was confirmed by qRT-PCR. Here, we discovered that FAM83A-AS1 was strongly expressed in ESCC tissues. FAM83A-AS1 abundance was associated with TNM stages and the differentiation grade of ESCC patients. The receiver operating characteristic curve (ROC) analysis indicated the high accuracy of FAM83A-AS1 in ESCC diagnosis. Functionally, inhibiting FAM83A-AS1 repressed cell proliferation, migration, and invasion in ESCC. In addition, we found that FAM83A-AS1 accelerated the cell cycle while inhibited cell apoptosis. Mechanistically, we found that FAM83A-AS1 regulated miR-214 expression, and there was a negative correlation between miR-214 and FAM83A-AS1 in ESCC. Rescue assay indicated that miR-214 could impair the suppressing effect of cell migration induced by FAM83A-AS1 depletion. Furthermore, CDC25B was a direct target of miR-214, and FAM83A-AS1 enhanced CDC25B expression while miR-214 positively CDC25B expression in ESCC. Collectively, we concluded that FAM83A-AS1 facilitated ESCC progression by regulating the miR-214/CDC25B axis. Our study showed FAM83A-AS1 may act as a promising target for ESCC diagnosis and therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.54007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797654PMC
January 2021

Adjuvant albumin-bound paclitaxel combined with S-1 vs. oxaliplatin combined with capecitabine after D2 gastrectomy in patients with stage III gastric adenocarcinoma: a phase III multicenter, open-label, randomized controlled clinical trial protocol.

BMC Cancer 2021 Jan 12;21(1):56. Epub 2021 Jan 12.

Department of Abdominal Surgery, Zhejiang Cancer Hospital (University of Chinese Academy of Sciences Cancer Hospital), Zhejiang, China.

Background: Surgery is the only treatment option for operable gastric cancer. The CLASSIC and ACTS-GC studies showed that the 5-year overall survival (OS) of patients with stage III gastric cancer undergoing D2 gastrectomy is still very low. Whether adjuvant nanoparticle albumin-bound paclitaxel (nab-paclitaxel) combined chemotherapy is more effective than the XELOX standard adjuvant chemotherapy in patients with stage III gastric cancer has not been confirmed.

Methods: This is a multicenter, open-label, phase III clinical study. In this trial, 616 patients with locally advanced stage III gastric cancer that underwent curative D2 radical surgery and achieved R0 are planned to be included. Patients will be randomized 1:1 to nab-paclitaxel combined with S-1 (AS) vs. oxaliplatin combined with capecitabine (XELOX). XELOX group: Patients assigned to the XELOX group received eight 3-week cycles of oral capecitabine (1000 mg/m) twice daily on days 1-14 of each cycle plus intravenous oxaliplatin 130 mg/m on day 1 of each cycle. AS group: AS group received eight 3-week cycles of oral S-1 (80-120 mg) (< 1.25 m, 40 mg; 1.25 to < 1.5 m, 50 mg; and > 1.5 m, 60 mg) twice daily on days 1-14 plus intravenous nab-paclitaxel 120 mg/m on days 1 and 8 of each cycle. The primary endpoint was the 3-year disease-free survival (3-year-DFS) defined as the time from randomisation to the time of recurrence of the original gastric cancer, development of a new gastric cancer, or death from any cause. The secondary endpoints were the overall survival, (defined as the time from the date of randomisation to date of death from any cause) and safety (any adverse event).

Discussion: Compared with previous studies, this study includes nab-paclitaxel based on S-1 adjuvant chemotherapy, which is expected to achieve better efficacy and lower toxicity than the standard treatment. This study is the first clinical study to evaluate the safety and efficacy of nab-paclitaxel combined with S-1 in patients with stage III gastric cancer after D2 radical resection.

Trial Registration: This clinical trial has been registered with ClinicalTrials.gov, registration number: NCT04135781 , on October 20th, 2019.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-020-07772-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802165PMC
January 2021

Cadmium and molybdenum co-induce pyroptosis via ROS/PTEN/PI3K/AKT axis in duck renal tubular epithelial cells.

Environ Pollut 2021 Mar 30;272:116403. Epub 2020 Dec 30.

Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, PR China. Electronic address:

Cadmium (Cd) and excess molybdenum (Mo) are harmful to animals, but the combined nephrotoxic mechanism of Cd and Mo in duck remains poorly elucidated. To assess joint effects of Cd and Mo on pyroptosis via ROS/PTEN/PI3K/AKT axis in duck renal tubular epithelial cells, cells were cultured with 3CdSO·8HO (4.0 μM), (NH)MoO·4HO (500.0 μM), MCC950 (10.0 μM), BHA (100.0 μM) and combination of Cd and Mo or Cd, Mo and MCC950 or Cd, Mo and BHA for 12 h, and the joint cytotoxicity was explored. The results manifested that toxicity of non-equitoxic binary mixtures of Mo and Cd exhibited synergic interaction. Mo or/and Cd elevated ROS level, PTEN mRNA and protein levels, and decreased PI3K, AKT and p-AKT expression levels. Simultaneously, Mo or/and Cd upregulated ASC, NLRP3, NEK7, Caspase-1, GSDMA, GSDME, IL-18 and IL-1β mRNA levels and Caspase-1 p20, NLRP3, ASC, GSDMD protein levels, increased the percentage of pyroptotic cells, LDH, NO, IL-18 and IL-1β releases as well as relative conductivity. Moreover, NLRP3 inhibitor MCC950 and ROS scavenger BHA could ameliorate the above changed factors induced by Mo and Cd co-exposure. Collectively, our results reveal that combination of Mo and Cd synergistically cause oxidative stress and trigger pyroptosis via ROS/PTEN/PI3K/AKT axis in duck tubular epithelial cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.116403DOI Listing
March 2021

Study of the photodegradation of 2-chlorobenzoic acid by TiO and the effects of eutrophicated water on the reaction.

Saudi J Biol Sci 2021 Jan 21;28(1):163-169. Epub 2020 Sep 21.

College of Marine and Environment Science, Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin Marine Environmental Protection and Restoration Technology Engineering Center, Tianjin University of Science and Technology, Tianjin 300457, China.

The photodegradation of 2-chlorobenzoic acid (2-CBA) in suspensions of TiO was examined under different operational parameters. The optimal condition could be obtained through the experiment, i.e. that the concentration of 2-CBA was 30 mg/L and the dosing quantity of TiO was 0.01 g under UV light in the case of pH 3.5. Above reaction process was in accordance with first order kinetics model. The influence on photocatalytic degradation caused by typical anions in eutrophicated water body such as NO and HPO was explored in this work, which revealed that both two anions had inhibitory effect on the degradation process. In addition, alcohol was introduced into the process to identify the degradation mechanism of 2-CBA with TiO, and the reaction route of 2-CBA could be predicted through the analysis on the intermediate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sjbs.2020.09.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783630PMC
January 2021

Clinical and Pathologic Feature of Patients With Early Versus Late Active Antibody-Mediated Rejection After Kidney Transplantation: A Single-Center Experience.

Transplant Proc 2021 Jan 5. Epub 2021 Jan 5.

Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), Guangzhou, China. Electronic address:

Objective: Active antibody-mediated rejection (aABMR), particularly late aABMR, remains a major challenge for long-term renal allograft survival. This single-center retrospective study aimed to compare clinical features between early vs late aABMR and to identify risk factors for allograft failure among patients with aABMR.

Method: Forty-one patients diagnosed with aABMR at our hospital were included and were divided into 2 groups: early aABMR (≤6 months; n = 10) vs late aABMR (>6 months; n = 31) based on the time from transplant to diagnosis. Their clinical and pathologic data were compared. This study was performed in compliance with the Helsinki Congress and the Declaration of Istanbul.

Results: Of 10 patients with early aABMR, none had allograft failure, whereas 8 of 31 patients with late aABMR had developed allograft failure at the time of follow-up (25.8%). At the time of biopsy, patients with early aABMR had higher positive grade in urine occult blood test than patients with late aABMR (P = .01); however, the late aABMR group displayed more intensive interstitial fibrosis and tubular atrophy (P = .03) and more frequent HLA-DQ-type donor-specific antibodies. Interestingly, donor-specific antibody conversion from positive to negative was not associated with C4d grade but was correlated with time from transplant to biopsy. Multivariate Cox regression analysis indicated that high levels of serum creatinine or proteinuria and concomitant T-cell-mediated rejection were independent risk factors for allograft failure in patients with aABMR.

Conclusion: These data not only confirm that early aABMR has better clinical outcomes than late aABMR but highlight the importance of early diagnostic biopsy and early therapeutic interventions in ABMR, particularly in patients with high levels of serum creatinine or proteinuria in the early posttransplant phase.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.transproceed.2020.11.004DOI Listing
January 2021

Classification Tree-Based Machine Learning to Visualize and Validate a Decision Tool for Identifying Malnutrition in Cancer Patients.

JPEN J Parenter Enteral Nutr 2021 Jan 8. Epub 2021 Jan 8.

Department of Clinical Nutrition, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Background: The newly-proposed Global Leadership Initiative on Malnutrition (GLIM) framework is promising to gain global acceptance for diagnosing malnutrition. However, the role of machine learning in facilitating its application in clinical practice remains largely unknown.

Methods: We performed a multicenter, observational cohort study including 3998 patients with cancer. Baseline malnutrition was defined using the GLIM criteria, and the study population was randomly divided into a derivation group (n = 2998) and a validation group (n = 1000). A Classification and Regression Trees (CART) algorithm was used to develop a decision tree for classifying the severity of malnutrition in the derivation group. The model performance was evaluated in the validation group.

Results: The GLIM criteria diagnosed 588 patients (14.7%) with moderate malnutrition and 532 patients (13.3%) with severe malnutrition among the study population. The CART cross-validation identified five key predictors for the decision tree construction, including age, weight loss within six months, body mass index, calf circumference and the nutritional risk screening 2002 (NRS2002) score. The decision tree showed high performance, with an area under curve (AUC) of 0.964 (Kappa = 0.898, P<0.001, Accuracy = 0.955) in the validation group. Subgroup analysis showed that the model had apparently good performance in different cancers. Among the five predictors constituting the tree, age contributed the least to the classification power.

Conclusion: Using the machine learning, we visualized and validated a decision tool based on the GLIM criteria that can be conveniently used to accelerate the pre-treatment identification of malnutrition in patients with cancer. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jpen.2070DOI Listing
January 2021

Maize transcription factor ZmEREB20 enhanced salt tolerance in transgenic Arabidopsis.

Plant Physiol Biochem 2021 Feb 28;159:257-267. Epub 2020 Dec 28.

Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 611130, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China. Electronic address:

Soil salinity severely limits agricultural crop production worldwide. As one of the biggest plant specific transcription factor families, AP2/ERF members have been extensively studied to regulate plant growth, development and stress responses. However, the role of AP2/ERF family in maize salt tolerance remains largely unknown. In this study, we identified a maize AP2-ERF family member ZmEREB20 as a positive salinity responsive gene. Overexpression of ZmEREB20in Arabidopsis enhanced ABA sensitivity and resulted in delayed seed germination under salt stress through regulating ABA and GA related genes. ZmEREB20 overexpression lines also showed higher survival rates with elevated ROS scavenging toward high salinity. Furthermore, root hair growth inhibition by salt stress was markedly rescued in ZmEREB20 overexpression lines. Auxin transport inhibitor TIBA drastically enhanced root hair growth in ZmEREB20 overexpression Arabidopsis under salt stress, together with the increased expression of auxin-related genes, ion transporter genes and root hair growth genes by RNA-seq analysis. ZmEREB20 positively regulated salt tolerance through the molecular mechanism associated with hormone signaling, ROS scavenging and root hair plasticity, proving the potential target for crop breeding to improve salt resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2020.12.027DOI Listing
February 2021

Anti-inflammatory effect of IL-1ra-loaded dextran/PLGA microspheres on Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages in vitro and in vivo in a rat model of periodontitis.

Biomed Pharmacother 2021 Feb 28;134:111171. Epub 2020 Dec 28.

Department of Periodontology, Hospital of Stomatology, Jilin University, Changchun, 130021, People's Republic of China. Electronic address:

Periodontitis is a multifactorial chronic infectious disease leading to a host immune response involving inflammatory cytokines, especially IL-1β, which is the main reason for further developing this disease. IL-1 receptor antagonist (IL-1ra) binds IL-1 receptor, inhibiting IL-1β signaling and reducing the levels of other cytokines closely related to periodontitis, such as IL-6 and TNF-α. Therefore, the use of IL-1ra to inhibit periodontitis development in a system, ensuring its sustained release, might be an effective way to combat this disease. Hence, in this study, a novel IL-1ra-loaded dextran/PLGA microsphere was developed to allow the sustained release of IL-1ra and enhance the anti-inflammatory properties. Therefore, this study's purposes were to develop a novel periodontal treatment for inhibition and treatment of periodontitis and evaluate the sustained-release effect and anti-inflammatory properties of IL-1ra-loaded dextran/PLGA microspheres in vitro by cell experiments and in vivo by animal experiments. The results showed that IL-1ra-loaded dextran/PLGA microspheres were non-toxic both in vitro and in vivo and could be used as a safe and effective treatment. In addition, these microspheres could significantly prolong the half-life of IL-1ra drug, exerting a useful anti-inflammatory effect in macrophages stimulated with P. gingivalis lipopolysaccharide and in rats with periodontitis. In conclusion, IL-1ra-loaded dextran/PLGA microsphere might be a useful tool to combat periodontal disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.111171DOI Listing
February 2021

LncRNA MIR205HG Drives Esophageal Squamous Cell Carcinoma Progression by Regulating miR-214/SOX4 Axis.

Onco Targets Ther 2020 22;13:13097-13109. Epub 2020 Dec 22.

Department of Medical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Background: Esophageal squamous cell carcinoma (ESCC) is a common and fatal malignancy, which has posed a great challenge to public health, especially in China. Dysregulation of long non-coding RNAs is involved in the occurrence, development, invasion, and metastasis of multiple cancers including ESCC. However, little is known about the function of MIR205HG in ESCC.

Methods: We used qRT-PCR to detect the expression level of MIR205HG, miR-214, and SOX4 in human ESCC tissues and cell lines. Loss-of-functional assays were performed to test the impact of MIR205HG on cell proliferation, metastasis, and apoptosis process via CCK-8, transwell, and flow cell cytometry assays. Additionally, the downstream molecular mechanism of MIR205HG in ESCC was explored.

Results: Here, we found MIR205HG was substantially up-regulated in ESCC, and there was a positive correlation between MIR205HG expression and tumor size and lymphatic metastasis of ESCC patients. Inhibition of MIR205HG attenuated cell proliferation, migration, and invasion. Silencing MIR205HG increased G1 phase cell counts and decreased S phase cell counts, along with increased apoptotic cell populations. Notably, the rescue assays indicated that miR-214 could partly reverse the influence of MIR205HG on ESCC cell migration. We also found that SOX4 was a direct target mRNA of miR-214, and MIR205HG could act as a molecular sponge to regulate SOX4 expression in ESCC.

Conclusion: Taken together, our findings demonstrate that MIR205HG promotes ESCC progression by regulating the miR-214/SOX4 axis. MIR205HG may be a novel candidate target for ESCC diagnosis and therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S286627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764791PMC
December 2020