Publications by authors named "Chandra Kishore"

4 Publications

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Epigenetic regulation and promising therapies in colorectal cancer.

Authors:
Chandra Kishore

Curr Mol Pharmacol 2021 Jan 25. Epub 2021 Jan 25.

Department of Biotechnology, Indian Institute of Technology Madras, Life Science Building, Fatki Kutti, Madhepur, Madhubani, Patna-847408, Bihar. India.

The recent developments in epigenetics have shown a very important role of epigenetic changes in cancer initiation, development, and progression. Some of the important histone modifications shown to occur are methylation, acetylation, phosphorylation, citrullination, sumoylation, ADP ribosylation, deamination, ubiquitination, formylation, O-GlcNAcylation, propionylation, butyrylation, proline isomerization, and crotonylation but most of the studies in past had limited their studies mainly on histone methylation, acetylation, and phosphorylation. Modification of DNA strand by hypermethylation and hypomethylation regulates genomic instability and promotes cancer. Colorectal cancer involves multiple changes in epigenetic marks present on histone residues and DNA, which in collaboration with genetic changes, drives cancer progression. In this review paper, basic concepts of epigenetics relevant to cancer development are discussed followed by its significance in understanding the mechanism of colon carcinogenesis. Some of the epigenetic target based drugs are also discussed in the relevant sections to give an idea of the potential promises of epigenetics for colorectal cancer treatment.
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http://dx.doi.org/10.2174/1874467214666210126105345DOI Listing
January 2021

Current advancements and future perspectives of immunotherapy in colorectal cancer research.

Eur J Pharmacol 2021 Feb 29;893:173819. Epub 2020 Dec 29.

Boral Tripursundari Road, Kolkata, 700154, West Bengal, India.

5-Fluorouracil (5-FU) is the first-line chemotherapy drug for colorectal cancer but most of the patients get resistant to the drug on a longer course of treatment. After the successful use of immunotherapy in melanoma treatment, it was explored with enthusiasm in different types of solid cancers including colorectal cancer. Nivolumab and pembrolizumab (Programmed cell death-1 blocking antibodies) have shown efficacy in the mismatch repair deficient high microsatellite instability (dMMR-MSI-H) subtype of metastatic colorectal cancer (CRC) patients. Immunotherapy has shown long time remission in a subset of metastatic CRC patients. The molecular mechanism and emerging roles of immunotherapy in colorectal cancer are explored in this review article and future directions for the proper utilization of the development in immunobiology are suggested.
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http://dx.doi.org/10.1016/j.ejphar.2020.173819DOI Listing
February 2021

Vitamin K3 (menadione) suppresses epithelial-mesenchymal-transition and Wnt signaling pathway in human colorectal cancer cells.

Chem Biol Interact 2019 Aug 22;309:108725. Epub 2019 Jun 22.

,Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, India. Electronic address:

Tumor recurrence and metastasis decrease the survival rate of colorectal cancer (CRC) patients. Menadione reduces the numbers and incidences of 1,2-dimethylhydrazine induced colon tumors in mouse but the mechanism of anticancer activity of menadione in colorectal cancer is not very clear. Since Wnt signaling is constitutively active in CRC and it aggravates the epithelial mesenchymal transition (EMT), the regulation of EMT and Wnt signaling by menadione (vitamin K3) was investigated in CRC cells. Menadione showed cytotoxicity against human CRC cells (SW480 and SW620) and human primary colon cancer cells but was relatively ineffective against the cells from human normal colon (CRL-1790) and human primary colon epithelial cells. Menadione suppressed invasion, migration and epithelial-mesenchymal transition in human CRC cells by upregulating the expression of E-cadherin (CDH1), ZO-1 and downregulating that of N-cadherin (CDH2), Vimentin (VIM), ZEB1, MMP2 and MMP9. Menadione decreased TOPFlash/FOPFlash luciferase activity and expression of several downstream targets of Wnt signaling and coactivators such as β-catenin (CTNNB1), TCF7L2, Bcl9l, p300 (EP300) and cyclin D1 (CCND1) was suppressed. Menadione induced differentiation and increased apoptotic cell population in SubG0 phase of cell cycle in SW480 and SW620 cells. The ability of menadione to suppress EMT, migration, invasion, Wnt signaling, cell proliferation and induce Sub G0 arrest, highlights its potential to be considered for intensive preclinical and clinical investigation in CRC.
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http://dx.doi.org/10.1016/j.cbi.2019.108725DOI Listing
August 2019

Fabrication of solution processed carbon nanotube embedded polyvinyl alcohol composite film for non-volatile memory device.

J Nanosci Nanotechnol 2014 Mar;14(3):2381-7

Carbon nanotubes (CNTs) were synthesized by chemical vapor deposition using nickel coated stainless steel prepared by electrophoretic deposition. CNTs were embedded in polyvinyl alcohol (PVA) which acts as an organic insulator to fabricate Si/PVA/CNT/PVA/Al Metal-Insulator-Semiconductor type memory devices. The effect of CNT content in the charge storage capacity of PVA-CNT composite film was investigated. The hysteresis obtained from the capacitance-voltage (CV) measurement resulted in a memory window of 1.9 V with 3% CNT loading with the gate voltage sweep of +/- 6 V at 1 MHz under room temperature. The memory window of the devices was due to electron injection into the CNT charge storage elements from the top electrode through PVA. The extensive pi-conjugation along the CNT axis traps the electrons in the CNT network. The ON/OFF state current ratio of Si/Al/PVA-CNT/AI device with 3% CNT in PVA demonstrated significantly a lower turn-on voltage of -1 V and a higher ON/OFF state current ratio of 10(7). The non-volatile and reprogrammable switching behavior of the device demonstrated the characteristic of a rewritable memory.
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http://dx.doi.org/10.1166/jnn.2014.8489DOI Listing
March 2014