Publications by authors named "Chan Li"

282 Publications

Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1.

Evid Based Complement Alternat Med 2021 26;2021:2136076. Epub 2021 Aug 26.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.

Methamphetamine (Meth) is a highly addictive substance and the largest drug threat across the globe. There is evidence to indicate that Meth use has serious damage on central nervous system (CNS) and heart in several animal and human studies. However, the connection in the process of Meth addiction between these two systems has not been determined. Emerging data suggest that extracellular vesicles (EVs) carrying behavior-altering microRNA (miRNAs) play a crucial role in cell communication between CNS and peripheral system. Rhynchophylline (Rhy), an antiaddictive alkaloid, was used to protect the brain and heart from Meth-induced damage, which has caught our attention. Here, we used Meth-dependent conditioned place preference (CPP) animal model and cell model to verify the protective effect of Rhy-treated EVs. Further, small RNA sequencing analysis, qPCR, dual-luciferase reporter assay, and transfection test were used to identify the key EVs-encapsulated miRNAs, isolated from cultured H9c2 cells with different treatments, involved in the therapeutic effect and the underlying mechanisms of Rhy. The results demonstrate that Rhy-treated EVs exert protective effects against Meth dependence through the pathway of miR-183-5p-neuregulin-1 (NRG1). Our collective findings provide novel insights into the roles of EVs miRNAs in Meth addiction and support their potential application in the development of novel therapeutic approaches.
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http://dx.doi.org/10.1155/2021/2136076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416368PMC
August 2021

Bioinformatic Analysis for Potential Biomarkers and Therapeutic Targets of T2DM-related MI.

Authors:
Chan Li Zhaoya Liu

Int J Gen Med 2021 10;14:4337-4347. Epub 2021 Aug 10.

Department of Geriatrics, Third Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Background: Type 2 diabetes mellitus (T2DM), a major risk factor of coronary heart disease, is associated with an approximately twofold increase in the risk of myocardial infarction (MI). We studied co-expressed genes to demonstrate relationships between DM and MI and revealed the potential biomarkers and therapeutic targets of T2DM-related MI.

Methods: DM and MI-related differentially expressed genes (DEGs) were identified by bioinformatic analysis, Gene Expression Omnibus (GEO) datasets GSE42148 and GSE61144 of MI patients, and the normal control and GSE26168 and GSE15932 of DM patients and normal controls, respectively. Further target prediction and network analysis method were used to detect protein-protein interaction (PPI) networks, gene ontology (GO) terms, and pathway enrichment of DEGs. Co-expressed DEGs of T2DM-related MI were analyzed as well.

Results: We identified 210 upregulated and 127 downregulated DEGs in T2DM, as well as 264 upregulated and 242 downregulated DEGs in MI. Eighteen upregulated and four downregulated DEGs were identified as co-DEGs of T2DM and MI. Functional analysis revealed that T2DM-related DEGs were mostly enriched in the viral process and ubiquitin-mediated proteolysis, while MI-related DEGs were mostly enriched in protein phosphorylation and TNF signaling pathway. , , , , , , , and were recognized as the hub genes of the co-DEGs with acceptable diagnostic values in T2DM and MI datasets. Adenosine receptor agonist IB-MECA was predicted to be a potential drug for T2DM-related MI with the highest CMap connectivity score.

Conclusion: Our study identified that the co-DEGs of , , , , , , , and are significantly associated with novel biomarkers involved in T2DM-related MI. However, more experimental research and clinical trials are demanded to verify our results.
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http://dx.doi.org/10.2147/IJGM.S325980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370113PMC
August 2021

Association Between the Admission Serum Bicarbonate and Short-Term and Long-Term Mortality in Acute Aortic Dissection Patients Admitted to the Intensive Care Unit.

Int J Gen Med 2021 5;14:4183-4195. Epub 2021 Aug 5.

Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Objective: Serum bicarbonate (HCO3) level is strongly related to multiple cardiovascular complications. Currently, there is no study evaluating the prognostic ability of serum HCO3 level in intensive care unit (ICU) patients with acute aortic dissection (AAD). Hence, this study was to assess the relationship between admission serum HCO3 level and clinical outcomes in patients with AAD.

Design Settings And Participants: Clinical data were extracted from the MIMIC-III database. Cox proportional hazards models and Kaplan-Meier (KM) survival curve were used to evaluate the association between serum HCO3 levels and short- and long-term mortality in ICU patients with AAD. The subgroup analysis and the receiver operating characteristic (ROC) curve analysis and further KM survival curve based on best cut-off value were applied to assessment of the performance of HCO3 in predicting the mortality in each period (30 days, 90 days, 1 year and 5 years).

Main Results: Firstly, 336 eligible patients were trisected to low-HCO3 level group (<22 mmol/L), mid-HCO3 level group (22-24 mmol/L) and high-HCO3 level group (>24 mmol/L). Then, in multivariate analysis, the serum HCO3 of low levels (<22 mmol/L) was a significant risk predictor of all-cause mortality in 30 days, 90 days, 1 year and 5 years. Subgroup analyses indicated that there is no interaction in most strata. Finally, areas under ROC curve ranged from 0.60 to 0.69.

Conclusion: The low HCO3 serum level measured at ICU admission significantly predicts short-term and long-term mortality in AAD patients.
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http://dx.doi.org/10.2147/IJGM.S321581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352635PMC
August 2021

Biomagnification and trophic transfer of total mercury and methylmercury in a sub-tropical montane forest food web, southwest China.

Chemosphere 2021 Aug 24;277:130371. Epub 2021 Mar 24.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China. Electronic address:

Little is known about the bioaccumulation and trophic transfer of total mercury (THg) and methylmercury (MeHg) via food webs in terrestrial ecosystems, especially in subtropical forest ecosystems. In the present study, THg and MeHg were determined as well as the carbon (δC) and nitrogen (δN) isotope composition in samples of soils, plants, invertebrates, and songbird feathers to construct food webs in a remote subtropical montane forest in Mt. Ailao, southwest China and assess the bioaccumulation, biomagnification, and trophic transfer of Hg. Results showed that the trophic levels (TLs) of all consumers ranged from 0.8 to 3.3 and followed the order of songbirds > spiders > omnivorous insects > herbivorous insects > plants, and THg and MeHg exhibited a clear biomagnification up the food chain from plants-herbivorous/omnivorous insects-spiders-songbirds. The lowest MeHg concentration was observed in pine needles ranged from 0.104 to 0.949 ng g with only a 1.6% ratio of MeHg to THg (MeHg%), while the highest MeHg concentrations ranged from 425 to 5272 ng g in songbirds with MeHg% values of up to 96%. High values of trophic magnification slope (TMS) for THg (0.22) and MeHg (0.38) were observed in plant-invertebrate-songbird food chain, verifying the significant bioaccumulation of Hg, particularly MeHg, in the remote subtropical forest ecosystem. This study confirmed the production and efficient biomagnification of MeHg in remote subtropical montane forest and the significant bioaccumulation of MeHg in terrestrial top predators.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130371DOI Listing
August 2021

A Bibliometric Analysis of 14,822 Researches on Myocardial Reperfusion Injury by Machine Learning.

Int J Environ Res Public Health 2021 08 3;18(15). Epub 2021 Aug 3.

Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

Myocardial ischemia is the major cause of death worldwide, and reperfusion is the standard intervention for myocardial ischemia. However, reperfusion may cause additional damage, known as myocardial reperfusion injury, for which there is still no effective therapy. This study aims to analyze the landscape of researches concerning myocardial reperfusion injury over the past three decades by machine learning. PubMed was searched for publications from 1990 to 2020 indexed under the Medical Subject Headings (MeSH) term "myocardial reperfusion injury" on 13 April 2021. MeSH analysis and Latent Dirichlet allocation (LDA) analyses were applied to reveal research hotspots. In total, 14,822 publications were collected and analyzed in this study. MeSH analyses revealed that time factors and apoptosis were the leading terms of the pathogenesis and treatment of myocardial reperfusion injury, respectively. In LDA analyses, research topics were classified into three clusters. Complex correlations were observed between topics of different clusters, and the prognosis is the most concerned field of the researchers. In conclusion, the number of publications on myocardial reperfusion injury increases during the past three decades, which mainly focused on prognosis, mechanism, and treatment. Prognosis is the most concerned field, whereas studies on mechanism and treatment are relatively lacking.
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http://dx.doi.org/10.3390/ijerph18158231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345983PMC
August 2021

A specialized myodural bridge named occipital-dural muscle in the narrow-ridged finless porpoise (Neophocaena asiaeorientalis).

Sci Rep 2021 Jul 29;11(1):15485. Epub 2021 Jul 29.

Department of Anatomy, College of Basic Medicine, Dalian Medical University, Dalian, China.

A dense bridge-like tissue named the myodural bridge (MDB) connecting the suboccipital muscles to the spinal dura mater was originally discovered in humans. However, recent animal studies have revealed that the MDB appears to be an evolutionarily conserved anatomic structure which may have significant physiological functions. Our previous investigations have confirmed the existence of the MDB in finless porpoises. The present authors conducted research to expound on the specificity of the MDB in the porpoise Neophocana asiaeorientalis (N.asiaeorientalis). Five carcasses of N.asiaeorientalis, with formalin fixation, were used for the present study. Two of the carcasses were used for head and neck CT scanning, three-dimensional reconstructions, and gross dissection of the suboccipital region. Another carcass was used for a P45 plastination study. Also, a carcass was used for a histological analysis of the suboccipital region and also one was used for a Scanning Electron Microscopy study. The results revealed that the MDB of the N.asiaeorientalis is actually an independent muscle originating from the caudal border of the occiput, passing through the posterior atlanto-occipital interspace, and then attaches to the cervical spinal dura mater. Thus the so called MDB of the N.asiaeorientalis is actually an independent and uniquely specialized muscle. Based on the origin and insertion of this muscle, the present authors name it the 'Occipital-Dural Muscle'. It appears that the direct pull of this muscle on the cervical spinal dura mater may affect the circulation of the cerebrospinal fluid by altering the volume of the subarachnoid space via a pumping action.
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http://dx.doi.org/10.1038/s41598-021-95070-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322066PMC
July 2021

Serum exosomes from young rats improve the reduced osteogenic differentiation of BMSCs in aged rats with osteoporosis after fatigue loading in vivo.

Stem Cell Res Ther 2021 07 27;12(1):424. Epub 2021 Jul 27.

National Clinical Research Center for Metabolic Diseases, Institute of Metabolism and Endocrinology, Central South University, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.

Background: Osteoporosis is a major public health concern for the elderly population and is characterized by fatigue load resulting in bone microdamage. The ability of bone mesenchymal stem cells (BMSCs) to repair bone microdamage diminishes with age, and the accumulation of bone microdamage increases the risk of osteoporotic fracture. There is a lack of effective means to promote the repair of bone microdamage in aged patients with osteoporosis. Exosomes have been shown to be related to the osteogenic differentiation of BMSCs. Here, we aimed to evaluate the changes in the osteogenic differentiation capacity of BMSCs in aged osteoporotic rats after fatigue loading and the treatment potential of serum exosomes from young rats.

Methods: The tibias of six aged osteoporotic rats were subjected to fatigue loading in vivo for 2 weeks, and the bone microdamage, microstructures, and mechanical properties were assessed. Subsequently, BMSCs were extracted to evaluate their proliferation and osteogenic differentiation abilities. In addition, the BMSCs of aged osteoporotic rats after fatigue loading were treated with serum exosomes from young rats under osteogenic induction conditions, and the expression of osteogenic-related miRNAs was quantified. The osteogenetic effects of miRNA-19b-3p in exosomes and the possible target protein PTEN was detected.

Results: Obvious bone microdamage at the fatigue load stress point, the bone microstructure and biomechanical properties were not obviously changed. A decreased osteogenic differentiation ability of BMSCs was observed after fatigue loading, while serum exosomes from young rats highly expressing miRNA-19b-3p improved the decreased osteogenic differentiation ability of BMSCs. Transfection with miRNA-19b-3p mimic could promote osteoblastic differentiation of BMSCs and decreased the expression of PTEN. After transfection of miRNA-19b-3p inhibitor, the promotional effect of exosomes on bone differentiation was weakened. Treatment with transfected exosomes increased the expression of PTEN.

Conclusion: Serum exosomes derived from young rats can improve the decreased osteogenic differentiation ability of BMSCs in aged rats with osteoporosis after fatigue loading and can provide a new treatment strategy for the repair of bone microdamage and prevention of fractures.
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http://dx.doi.org/10.1186/s13287-021-02449-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314589PMC
July 2021

Recent advance of ACE2 and microbiota dysfunction in COVID-19 pathogenesis.

Heliyon 2021 Jul 10;7(7):e07548. Epub 2021 Jul 10.

Department of Gastroenterology, Henan Key Laboratory of Children's Genetics and Metabolic Diseases, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, China.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the coronavirus disease 2019 (COVID-19) and has become the world's most pressing public health threat. Although not as common as respiratory symptoms, a substantial proportion of patients with COVID-19 presented the gastrointestinal symptoms. ACE2, as the receptor of SARS-CoV and SARS-CoV-2, is highly expressed in the epithelia of the epithelium cells in lung and intestine. In addition, ACE2 is essential for the innate immunity, amino acid transportation and the homeostasis of intestinal microecology. The composition of gut microbiota in COVID-19 patients was altered and concordant with inflammatory, which may explain the gastrointestinal symptoms in patients. Here we reviewed and discussed the evolving role for ACE2 and gut microbiota in SARS-CoV-2 infection which might provide innovative approaches to targeting ACE2 and gut microbiota for the COVID-19 therapy.
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http://dx.doi.org/10.1016/j.heliyon.2021.e07548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270732PMC
July 2021

mTORC1 Signaling Regulates Proinflammatory Macrophage Function and Metabolism.

J Immunol 2021 08 21;207(3):913-922. Epub 2021 Jul 21.

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD;

Metabolic programming is integrally linked to immune cell function. Nowhere is this clearer than in the differentiation of macrophages. Proinflammatory M1 macrophages primarily use glycolysis as a rapid energy source but also to generate antimicrobial compounds, whereas alternatively activated M2 macrophages primarily rely on oxidative phosphorylation for the longevity required for proper wound healing. mTOR signaling has been demonstrated to be a key regulator of immune cell metabolism and function. mTORC2 signaling is required for the generation of M2 macrophages, whereas the role of mTORC1 signaling, a key regulator of glycolysis, has been controversial. By using genetic deletion of mTORC1 signaling in C57BL/6 mouse macrophages, we observed enhanced M1 macrophage function in vitro and in vivo. Surprisingly, this enhancement occurred despite a significant defect in M1 macrophage glycolytic metabolism. Mechanistically, enhanced M1 function occurred because of inhibition of the class III histone deacetylases the sirtuins, resulting in enhanced histone acetylation. Our findings provide a counterpoint to the paradigm that enhanced immune cell function must occur in the presence of increased cellular metabolism and identifies a potential, pharmacologic target for the regulation of inflammatory responses.
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http://dx.doi.org/10.4049/jimmunol.2100230DOI Listing
August 2021

Genetic Analysis of Pediatric Primary Adrenal Insufficiency of Unknown Etiology: 25 Years' Experience in the UK.

J Endocr Soc 2021 Aug 11;5(8):bvab086. Epub 2021 May 11.

Genetics and Genomic Medicine Research and Teaching Department, UCL Great Ormond Street Institute of Child Health, University College London, London, UK.

Context: Although primary adrenal insufficiency (PAI) in children and young people is often due to congenital adrenal hyperplasia (CAH) or autoimmunity, other genetic causes occur. The relative prevalence of these conditions is poorly understood.

Objective: We investigated genetic causes of PAI in children and young people over a 25 year period.

Design Setting And Participants: Unpublished and published data were reviewed for 155 young people in the United Kingdom who underwent genetic analysis for PAI of unknown etiology in three major research centers between 1993 and 2018. We pre-excluded those with CAH, autoimmune, or metabolic causes. We obtained additional data from NR0B1 (DAX-1) clinical testing centers.

Intervention And Outcome Measurements: Genetic analysis involved a candidate gene approach (1993 onward) or next generation sequencing (NGS; targeted panels, exomes) (2013-2018).

Results: A genetic diagnosis was reached in 103/155 (66.5%) individuals. In 5 children the adrenal insufficiency resolved and no genetic cause was found. Pathogenic variants occurred in 11 genes: (adrenocorticotropin receptor; 30/155, 19.4%), (DAX-1; 7.7%), (7.7%), (7.1%), (6.5%), (4.5%), (4.5%), (3.9%), (3.2%), (1.3%), and /steroidogenic factor-1 (SF-1; 0.6%). Additionally, 51 boys had variants identified through clinical testing. Although age at presentation, treatment, ancestral background, and birthweight can provide diagnostic clues, genetic testing was often needed to define the cause.

Conclusions: PAI in children and young people often has a genetic basis. Establishing the specific etiology can influence management of this lifelong condition. NGS approaches improve the diagnostic yield when many potential candidate genes are involved.
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http://dx.doi.org/10.1210/jendso/bvab086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266051PMC
August 2021

Phylogenetic position of Bupleurum sikangense inferred from the complete chloroplast genome sequence.

Gene 2021 Sep 25;798:145801. Epub 2021 Jun 25.

Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou 510515, China. Electronic address:

Bupleurum sikangense is an endemic species to China distributed in Xizang (Tibet), which has high saikosaponin content and potential medicinal value. Morphologically, it extremely resembles B. commelynoideum. In order to get a better understanding of the relationship between B. sikangense and B. commelynoideum, and on the phylogenetic status of the two species in the genus, the complete chloroplast (cp) genomes of them were sequenced. The genome organization, repeat sequences, codon usage, RNA-editing sites, and variation of their cp genomes revealed high similarity between the species. Some highly variable regions like trnK-UUU_rps16, rps16_trnQ-UUG, ndhC_trnV-UAC, petA_psbJ, accD_psaI, and petL_psbE were identified, providing potential molecular markers for differentiating the two species. Phylogenetic analysis indicated that B. commelynoideum has a closer relationship to B. chinese than that to B. sikangense. Overall, this study will not only improve our knowledge about cp genomes of these two species, and but also provide data for further research on species identification, safe medical application, conservation genetics, etc., of Bupleurum plants.
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http://dx.doi.org/10.1016/j.gene.2021.145801DOI Listing
September 2021

The Ameliorative Effects of Isorhynchophylline on Morphine Dependence Are Mediated Through the Microbiota-Gut-Brain Axis.

Front Pharmacol 2021 8;12:526923. Epub 2021 Jun 8.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.

Morphine abuse is a global public health problem. Increasing evidence has shown that gut microbiota dysbiosis plays an important role in several central nervous system diseases. However, whether there is an association between gut microbiota and morphine dependence remains unclear. In this study, the effects of isorhynchophylline on morphine dependence were evaluated based on the microbiota-gut-brain axis (MGBA). The results showed that isorhynchophylline could reverse the changes in alpha and beta diversity, composition, and richness of the intestinal flora occurring in morphine-dependent zebrafish, as well as the morphine-induced changes in the expression of MGBA-related genes in BV2 cells and the brain and intestine of zebrafish. Based on the results, we then used antibiotics to evaluate whether disrupting the gut microbiota would affect morphine addiction in zebrafish. The results showed that the antibiotic-induced intestinal floral imbalance changed the behavior of morphine-dependent zebrafish, the characteristics of the zebrafish intestinal flora, and the expression of MGBA-related genes in the zebrafish brain and intestine. Importantly, we also show that, following antibiotic administration, the ameliorative effects of isorhynchophylline on morphine addiction were lost. Together, our results indicate that the gut microbiota interacts with the brain, and dysbiosis of the intestinal flora may affect the efficacy of isorhynchophylline in the body. Our findings provide a novel framework for understanding the mechanisms of morphine addiction through the MGBA and may provide new therapeutic strategies for the use of Chinese medicines in the prevention of drug addiction.
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http://dx.doi.org/10.3389/fphar.2021.526923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218633PMC
June 2021

Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway.

Redox Biol 2021 09 6;45:102031. Epub 2021 Jun 6.

Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, 41008, Changsha, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. Electronic address:

Reactive oxygen species (ROS) derived from NADPH oxidases (NOX) plays an essential role in advanced glycation end products (AGEs)-induced diabetic vascular endothelial dysfunction. Peroxidasin (PXDN, VPO1) is one member of peroxidases family that catalyzes hydrogen peroxide (HO) to hypochlorous acid (HOCl). This present study aimed to elucidate the role of PXDN in promoting vascular endothelial dysfunction induced by AGEs in diabetes mellitus. We found that, compared to non-diabetic (db/m) mice, PXDN expression was notably increased in db/db mice with impaired endothelium-dependent relaxation. Knockdown of PXDN in vivo through tail vein injection of siRNA restored the impaired endothelium-dependent relaxation function of db/db mice which is accompanied with up-regulation of eNOS Ser1177 phosphorylation and NO production. AGEs significantly elevated expression of PXDN and 3-Cl-Tyr, but decreased phosphorylation of Akt and eNOS and NO release in HUVECs. All these effects induced by AGEs were remarkable alleviated by silencing PXDN with small interfering RNAs. In addition, HOCl treatment alone as well as HOCl added with Akt inhibitor MK2206 inhibited phosphorylation of Akt and eNOS, reducing NO production. More importantly,AGEs-induced up-regulation of PXDN and 3-Cl-Tyr with endothelial dysfunction were transformed by NOX2 silencing and HO scavengers. Thus, these results support the conclusion that PXDN promotes AGEs-induced diabetic vascular endothelial dysfunction by attenuating eNOS phosphorylation at Ser1177 via NOX2/HOCl/Akt pathway.
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http://dx.doi.org/10.1016/j.redox.2021.102031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192873PMC
September 2021

Effects of propofol on the proliferation and migration of liver cancer cells.

Exp Ther Med 2021 Jul 9;22(1):733. Epub 2021 May 9.

Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.

Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (CoCl)-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 µM propofol group; iii) 25 µM propofol group; iv) 50 µM propofol group; v) 100 µM propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under CoCl-induced hypoxia, the protein levels of hypoxia inducible factor-1α (HIF-1α) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1α were significantly upregulated following the treatment with 200 µM CoCl for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of CoCl. In conclusion, the findings of the present study revealed that propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions.
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http://dx.doi.org/10.3892/etm.2021.10165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138278PMC
July 2021

Anatomical basis of the support of fibula to tibial plateau and its clinical significance.

J Orthop Surg Res 2021 May 29;16(1):346. Epub 2021 May 29.

Department of Anatomy, College of Basic Medicine, Dalian Medical University, Dalian, 116044, China.

Background: The fibula is only indirectly involved in the composition of the human knee joint and has therefore been neglected in the research on knee osteoarthritis. Nonuniform settlement of the proximal tibia plateau is clinically defined as when the height of the medial tibial plateau is lower than that of the lateral side in medial compartment knee osteoarthritis (KOA). The non-uniform settlement of the proximal tibia plateau may be caused by fibular support on the lateral side. Orthopedic surgeons practice partial fibulectomy based on the clinical manifestation of nonuniform settlement, and this technique has been shown to reduce pain and improve function in patients with medial compartment KOA. However, this hypothesis of the mechanism of nonuniform settlement lacks an anatomical basis.

Methods: The P45 polyester plastination technique was used to prepare sections of the proximal tibiofibular joint to investigate the distribution of the bone trabeculae in the region of the lateral tibial plateau.

Results: There was uneven distribution of trabeculae in the lateral condyle of the tibia and the head and neck of the fibula. The fibula and the posterolateral cortex of the shaft of the tibia united to form an arch beam via the tibiofibular joint. Many thick, dense trabeculae were present in a longitudinal direction above the tibiofibular arch.

Conclusions: The fibula supports the lateral tibial plateau, and the trabeculae were concentrated above the tibiofibular arch.
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http://dx.doi.org/10.1186/s13018-021-02500-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164332PMC
May 2021

Gut Akkermansia muciniphila ameliorates metabolic dysfunction-associated fatty liver disease by regulating the metabolism of L-aspartate via gut-liver axis.

Gut Microbes 2021 Jan-Dec;13(1):1-19

Run Ze Laboratory for Gastrointestinal Microbiome Study, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.

The gut bacterium has been increasingly recognized for its therapeutic potential in treating metabolic disorders, including obesity, diabetes, and metabolicdysfunction-associated fatty liver disease (MAFLD). However, its underlying mechanism involved in its well-known metabolic actions needs further evaluation. The present study explored the therapeutic effect and mechanism of in intervening MAFLD by using a high-fat and high-cholesterol (HFC) diet induced obese mice model. Mice treated with efficiently reversed MAFLD in the liver, such as hepatic steatosis, inflammatory, and liver injury. These therapeutic effects persisted after long-term drug withdrawal and were slightly weakened in the antibiotics-treated obese mice. treatment efficiently increased mitochondrial oxidation and bile acid metabolism in the gut-liver axis, ameliorated oxidative stress-induced cell apoptosis in gut, leading to the reshaping of the gut microbiota composition. These metabolic improvements occurred with increased L-aspartate levels in the liver that transported from the gut. The administration of L-aspartate or in mice displayed the similar beneficial metabolic effects mentioned above and efficiently ameliorated MAFLD. Together, these data indicate that the anti-MAFLD activity of correlated with lipid oxidation and improved gut-liver interactions through regulating the metabolism of L-aspartate. could be a potential agent for clinical intervention in MAFLD.
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http://dx.doi.org/10.1080/19490976.2021.1927633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158032PMC
May 2021

Distributions of Total Mercury and Methylmercury in Dragonflies from a Large, Abandoned Mercury Mining Region in China.

Arch Environ Contam Toxicol 2021 Jul 23;81(1):25-35. Epub 2021 May 23.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China.

Dragonflies (Order Odonata) often are considered to be biosentinels of environmental contamination, e.g., heavy metals and/or persistent organic pollutants (POPs). Dragonflies (n = 439) belonging to 15 species of 8 genera were collected from an abandoned mercury (Hg) mining region in China to investigate the bioaccumulation of total Hg (THg) and methylmercury (MeHg). THg and MeHg concentrations in dragonflies varied widely within ranges of 0.06-19 mg/kg (average: 1.5 ± 2.2 mg/kg) and 0.02-5.7 mg/kg (average: 0.75 ± 0.65 mg/kg), respectively. THg and MeHg were positively correlated with bodyweight (THg: r = 0.10, P = 0.000; MeHg: r = 0.09, P = 0.000). Significant variations were observed among species, with the highest MeHg value (in Orthetrum triangulare) was fivefold higher than the lowest (in Pantala flavescens). These variations were consistent with those of nitrogen isotope (δN) values, indicating that increased δN, i.e., trophic levels, may reflect increased exposure and uptake of biomagnifying MeHg in dragonflies. A toxicological risk assessment found hazard quotients for specialist dragonfly-consuming birds of up to 7.2, which is 2.4 times greater than the permissible limit of 3, suggesting a potential toxicological risk of exposure.
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http://dx.doi.org/10.1007/s00244-021-00854-yDOI Listing
July 2021

PXDN reduces autophagic flux in insulin-resistant cardiomyocytes via modulating FoxO1.

Cell Death Dis 2021 04 26;12(5):418. Epub 2021 Apr 26.

Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Autophagy, a well-observed intracellular lysosomal degradation process, is particularly important to the cell viability in diabetic cardiomyopathy (DCM). Peroxidasin (PXDN) is a heme-containing peroxidase that augments oxidative stress and plays an essential role in cardiovascular diseases, while whether PXDN contributes to the pathogenesis of DCM remains unknown. Here we reported the suppression of cell viability and autophagic flux, as shown by autophagosomes accumulation and increased expression level of LC3-II and p62 in cultured H9C2 and human AC16 cells that treated with 400 μM palmitate acid (PA) for 24 h. Simultaneously, PXDN protein level increased. Moreover, cell death, autophagosomes accumulation as well as increased p62 expression were suppressed by PXDN silence. In addition, knockdown of PXDN reversed PA-induced downregulated forkhead box-1 (FoxO1) and reduced FoxO1 phosphorylation, whereas did not affect AKT phosphorylation. Not consistent with the effects of si-PXDN, double-silence of PXDN and FoxO1 significantly increased cell death, suppressed autophagic flux and declined the level of FoxO1 and PXDN, while the expression of LC3-II was unchanged under PA stimulation. Furthermore, inhibition of FoxO1 in PA-untreated cells induced cell death, inhibited autophagic flux, and inhibited FoxO1 and PXDN expression. Thus, we come to conclusion that PXDN plays a key role in PA-induced cell death by impairing autophagic flux through inhibiting FoxO1, and FoxO1 may also affect the expression of PXDN. These findings may develop better understanding of potential mechanisms regarding autophagy in insulin-resistant cardiomyocytes.
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http://dx.doi.org/10.1038/s41419-021-03699-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076187PMC
April 2021

Structure reveals the activation mechanism of the MC4 receptor to initiate satiation signaling.

Science 2021 05 15;372(6544):808-814. Epub 2021 Apr 15.

Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.

Obesity is a global epidemic that causes morbidity and impaired quality of life. The melanocortin receptor 4 (MC4R) is at the crux of appetite, energy homeostasis, and body-weight control in the central nervous system and is a prime target for anti-obesity drugs. Here, we present the cryo-electron microscopy (cryo-EM) structure of the human MC4R-G signaling complex bound to the agonist setmelanotide, a cyclic peptide recently approved for the treatment of obesity. The work reveals the mechanism of MC4R activation, highlighting a molecular switch that initiates satiation signaling. In addition, our findings indicate that calcium (Ca) is required for agonist, but not antagonist, efficacy. These results fill a gap in the understanding of MC4R activation and could guide the design of future weight-management drugs.
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http://dx.doi.org/10.1126/science.abf7958DOI Listing
May 2021

Bisphenol A Exposure Disrupts Organelle Distribution and Functions During Mouse Oocyte Maturation.

Front Cell Dev Biol 2021 23;9:661155. Epub 2021 Mar 23.

Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Bisphenol A (BPA) is one of the ubiquitous environmental endocrine disruptors (EEDs). Previous studies have shown that the reproduction toxicity of BPA could cause severe effects on the mammal oocytes and disturb the quality of mature oocytes. However, the toxic effects of BPA on the organelles of mouse oocytes have not been reported. In this study, to investigate whether BPA can be toxic to the organelles, we used different concentrations of BPA (50, 100, and 200 μM) to culture mouse oocytes . The results showed that 100 μM BPA exposure could significantly decrease the developmental capacity of oocytes. Then, we used the immunofluorescence staining, confocal microscopy, and western blotting to investigate the toxic effects of BPA on the organelles. The results revealed that mitochondrial dysfunction is manifested by abnormal distribution and decreased mitochondrial membrane potential. Moreover, the endoplasmic reticulum (ER) is abnormally distributed which is accompanied by ER stress showing increased expression of GRP78. For the Golgi apparatus, BPA-exposed dose not disorder the Golgi apparatus distribution but caused abnormal structure of Golgi apparatus, which is manifested by the decrease of GM130 protein expression. Moreover, we also found that BPA-exposed led to the damage of lysosome, which were shown by the increase of LAMP2 protein expression. Collectively, our findings demonstrated that the exposure of BPA could damage the normal function of the organelles, which may explain the reduced maturation quality of oocytes.
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http://dx.doi.org/10.3389/fcell.2021.661155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021768PMC
March 2021

PLCG2 as a potential indicator of tumor microenvironment remodeling in soft tissue sarcoma.

Medicine (Baltimore) 2021 Mar;100(11):e25008

Department of Anesthesiology, The second Hospital of Anhui Medical University, Hefei, China.

Abstract: The tumor microenvironment (TME) plays an important role in the occurrence and development of soft tissue sarcoma (STS). A number of studies have shown that to inhibit tumor growth, the TME can be remodeled into an environment unsuitable for tumor proliferation. However, a lack of understanding exists regarding the dynamic regulation of TME.In this study, we used CIBERSORT and ESTIMATE calculation methods from the Cancer Genome Atlas (TCGA) database to calculate the proportion of tumor infiltrating immune cells (TICs) and the number of immune and stromal components in 263 STS samples. Differential expression genes (DEGs) shared by Immune Score and Stromal Score were obtained via difference analysis. Univariate Cox regression analysis and construction of protein-protein interaction (PPI) networks were applied to the DEGs.Through intersection analysis of univariate COX and PPI, PLCG2 was determined as the indicator. Further analysis showed that PLCG2 expression was positively correlated with the survival of STS patients. Gene set enrichment analysis (GSEA) showed that genes in the highly expressed PLCG2 group were enriched in immune-related activities. In the low-expression PLCG2 group, genes were enriched in the E2F, G2M, and MYC pathways. Difference analysis and correlation analysis showed that CD8+ T cells, gamma delta T cells, monocytes, and M1 macrophages were positively correlated with PLCG2 expression, indicating that PLCG2 may represent the immune status of TME.Therefore, the level of PLCG2 may aid in determining the prognosis of STS patients, especially the status of TME. These data provide additional insights into the remodeling of TME.
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http://dx.doi.org/10.1097/MD.0000000000025008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982206PMC
March 2021

High-Normal Serum Magnesium and Hypermagnesemia Are Associated With Increased 30-Day In-Hospital Mortality: A Retrospective Cohort Study.

Front Cardiovasc Med 2021 10;8:625133. Epub 2021 Feb 10.

Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, China.

Magnesium, the fourth most abundant mineral nutrient in our body, plays a critical role in regulating ion channels and energy generation, intracardiac conduction, and myocardial contraction. In this study, we assessed the association of admission serum magnesium level with all-cause in-hospital mortality in critically ill patients with acute myocardial infarction (AMI). Clinical data were extracted from the eICU Collaborative Research Database (eICU-CRD). Only the data for the first intensive care unit (ICU) admission of each patient were used, and baseline data were extracted within 24 h after ICU admission. Logistic regression, Cox regression, and subgroup analyses were conducted to determine the relationship between admission serum magnesium level and 30-day in-hospital mortality in ICU patients with AMI. A total of 9,005 eligible patients were included. In the logistic regression analysis, serum magnesium at 2.2 to ≤2.4 and >2.4 mg/dl levels were both significant predictors of all-cause in-hospital mortality in AMI patients. Moreover, serum magnesium of 2.2 to ≤2.4 mg/dl showed higher risk of in-hospital mortality than magnesium of >2.4 mg/dl (adjusted odds ratio, 1.63 vs. 1.39). The Cox regression analysis yielded similar results (adjusted hazard ratio, 1.36 vs. 1.25). High-normal serum magnesium and hypermagnesemia may be useful and easier predictors for 30-day in-hospital mortality in critically ill patients with AMI.
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http://dx.doi.org/10.3389/fcvm.2021.625133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902876PMC
February 2021

Systematic Prioritization of Candidate Genes in Disease Loci Identifies as a Master Regulator of IFNγ Signaling in Celiac Disease.

Front Genet 2020 25;11:562434. Epub 2021 Jan 25.

Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.

Celiac disease (CeD) is a complex T cell-mediated enteropathy induced by gluten. Although genome-wide association studies have identified numerous genomic regions associated with CeD, it is difficult to accurately pinpoint which genes in these loci are most likely to cause CeD. We used four different approaches-Mendelian randomization inverse variance weighting, COLOC, LD overlap, and DEPICT-to integrate information gathered from a large transcriptomics dataset. This identified 118 prioritized genes across 50 CeD-associated regions. Co-expression and pathway analysis of these genes indicated an association with adaptive and innate cytokine signaling and T cell activation pathways. Fifty-one of these genes are targets of known drug compounds or likely druggable genes, suggesting that our methods can be used to pinpoint potential therapeutic targets. In addition, we detected 172 gene combinations that were affected by our CeD-prioritized genes in . Notably, 41 of these -mediated genes appear to be under control of one master regulator, (), and were found to be involved in interferon (IFN)γ signaling and MHC I antigen processing/presentation. Finally, we performed experiments in a human monocytic cell line that validated the role of as an immune regulator acting in . Our strategy confirmed the role of adaptive immunity in CeD and revealed a genetic link between CeD and IFNγ signaling as well as with MHC I antigen processing, both major players of immune activation and CeD pathogenesis.
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http://dx.doi.org/10.3389/fgene.2020.562434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868554PMC
January 2021

Use of a proforma to improve documentation of the post-take ward round and encourage initiation of the comprehensive geriatric assessment in the care of the older people's service.

Br J Hosp Med (Lond) 2021 Jan 28;82(1):1-6. Epub 2021 Jan 28.

Care of the Older People's Service, Royal London Hospital, Bart's Health NHS Trust, London, UK.

Aims/background: The post-take ward round is often the first time that a senior clinician reviews a patient on the acute medical take. Despite this, there is no official guidance regarding structure or documentation of the post-take ward round. The aim of this quality improve project was to develop a ward round proforma specifically tailored to the care of the older people's service to improve quality of documentation and to encourage initiation of the comprehensive geriatric assessment.

Methods: An initial audit was carried out assessing the documentation of key information and the initiation of the comprehensive geriatric assessment during the post-take ward round. A proforma was subsequently designed and implemented with the aims of improving the quality of documentation and increasing the number of patients for whom the comprehensive geriatric assessment was started. A repeat audit was conducted to assess the effectiveness of the proforma.

Results: The results demonstrated an improvement in documentation of all key information criteria and an increase in the initiation of the comprehensive geriatric assessment.

Conclusions: Use of a specifically tailored post-take ward round proforma improves the quality and consistency of documentation and encourages the initiation of the comprehensive geriatric assessment.
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http://dx.doi.org/10.12968/hmed.2020.0604DOI Listing
January 2021

Chinese Herbal Medicine for the Treatment of Depression: Effects on the Neuroendocrine-Immune Network.

Pharmaceuticals (Basel) 2021 Jan 14;14(1). Epub 2021 Jan 14.

School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

The neuroimmune and neuroendocrine systems are two critical biological systems in the pathogenesis of depression. Clinical and preclinical studies have demonstrated that the activation of the neuroinflammatory response of the immune system and hyperactivity of the hypothalamus-pituitary-adrenal (HPA) axis of the neuroendocrine system commonly coexist in patients with depression and that these two systems bidirectionally regulate one another through neural, immunological, and humoral intersystem interactions. The neuroendocrine-immune network poses difficulties associated with the development of antidepressant agents directed toward these biological systems for the effective treatment of depression. On the other hand, multidrug and multitarget Chinese Herbal Medicine (CHM) has great potential to assist in the development of novel medications for the systematic pharmacotherapy of depression. In this narrative essay, we conclusively analyze the mechanisms of action of CHM antidepressant constituents and formulas, specifically through the modulation of the neuroendocrine-immune network, by reviewing recent preclinical studies conducted using depressive animal models. Some CHM herbal constituents and formulas are highlighted as examples, and their mechanisms of action at both the molecular and systems levels are discussed. Furthermore, we discuss the crosstalk of these two biological systems and the systems pharmacology approach for understanding the system-wide mechanism of action of CHM on the neuroendocrine-immune network in depression treatment. The holistic, multidrug, and multitarget nature of CHM represents an excellent example of systems medicine in the effective treatment of depression.
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http://dx.doi.org/10.3390/ph14010065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830381PMC
January 2021

The complete chloroplast genome sequence of (Linnaeus) Urban.

Mitochondrial DNA B Resour 2020 May 22;5(3):2149-2150. Epub 2020 May 22.

Faculty of Medicinal Plants and Pharmacognosy, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, People's Republic of China.

The apiaceous species (Linnaeus) Urban is attractive not only to pharmaceutical researchers for its versatile medicinal uses, but also to botanists for its phylogenetic significance. We acquired its whole chloroplast genome (CP) through genome skimming. The CP genome of was 154,771 bp in length, including a large single-copy (LSC) region with 86176 bp, a small single copy (SSC) region with 18107 bp, and a pair of inverted repeats (IR) regions with 25,343 bp. The whole AT content of the CP genome was 62.3%. Phylogenomic analysis revealed that formed a separate clade sister to Saniculoideae and Apioideae species in the family Apiaceae. The work provides beneficial data for following researches on the genetic variation, species identification, phylogeny, and classification of
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http://dx.doi.org/10.1080/23802359.2020.1768922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781932PMC
May 2020

Connective Tissue Growth Factor in Digestive System Cancers: A Review and Meta-Analysis.

Biomed Res Int 2020 27;2020:8489093. Epub 2020 Dec 27.

Oncology Clinical Department, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 611731, China.

Aim: A meta-analysis was conducted to estimate the impact of connective tissue growth factor (CTGF) on outcomes in patients with digestive system cancers.

Methods: A systemic literature survey was performed by searching the Cochrane Library and PubMed databases for articles that evaluated the impact of CTGF on outcomes in patients with digestive system cancers. Hazard ratios and 95% confidence intervals were calculated for prognostic factors, overall and recurrence-free survival using RevMan 5.3 software.

Results: This meta-analysis was conducted to evaluate a total of 11 studies that included 1730 patients. The results showed that elevated CTGF expression was significantly correlated with advanced age, larger tumor size, multiple tumors, and vascular invasion. Subgroup analysis by cancer type revealed increased risk for lymph node metastasis and advanced tumor node metastasis (TNM) stage in gastric cancer, compared with colorectal cancer. An unfavorable effect of elevated CTGF levels on overall survival was found in patients with hepatocellular carcinoma and patients with gastric cancer, while survival was improved in colorectal cancer patients with high CTGF expression, compared to those with normal levels of CTGF.

Conclusions: Elevated CTGF expression may be a novel biomarker for disease status and predicted survival outcomes in patients with specific digestive system cancers.
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http://dx.doi.org/10.1155/2020/8489093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781715PMC
May 2021

Cadmium exposure as a key risk factor for residents in a world large-scale barite mining district, southwestern China.

Chemosphere 2021 Apr 21;269:129387. Epub 2020 Dec 21.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China. Electronic address:

Cadmium (Cd) contamination is easily generated during the mining and manufacturing of barium (Ba). In this study, concentrations of both Ba and Cd in rice, vegetables, pork, fish, drinking water, and soil samples from an active barite mining district were determined. Daily intakes of Ba and Cd, as well as corresponding health risks, were evaluated. The average total daily exposure doses of Cd were 0.0035 and 0.0012 mg/kg BW/day (geometric mean) in the mining zone (MZ) and the chemical plant zone (PZ), respectively. These values significantly exceed the provisional tolerable monthly intake (25 μg/kg BW/month, equal to 0.00083 mg/kg BW/day). Based on the daily exposure doses, vegetable consumption was the most significant Ba exposure route for residents, contributing around 66.1% of the total exposure. In contrast, rice consumption was the major Cd exposure pathway, accounting for about 85.6% of the total exposure. Although the geometric mean (0.17) and 95th percentile (P95, 0.75) of the total hazard quotient (HQ) for Ba were below the acceptable level (1), suggesting that there were no significant health effects caused by Ba exposure, Cd exposure was associated with significant health risks, with the geometric mean of the HQ (1.7) and the P95 (21) well above the acceptable limit (1), indicating the unacceptable non-carcinogenic risk of Cd exposure. In summary, high Cd exposure risk, rather than Ba, was observed for populations living in a large-scale active Ba mining area.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129387DOI Listing
April 2021

The complete chloroplast genome sequence of (Moraceae).

Mitochondrial DNA B Resour 2019 Nov 13;4(2):4041-4042. Epub 2019 Nov 13.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.

The dry root (Radix Fici Hirtae) of has been used as a traditional herbal medicine in Ling nan regions of China for a long time. As its large market demand, the wild resources of have sharply reduced. It is necessary to conduct the study of conservation genetics. However, there is still lack of complete genome information for the research on evolutionary biology, population genetics and phylogeography of this species. Here, we sequenced the complete chloroplast (CP) genome of using Next Generation Sequencing technology (NGS). The CP genome of is 160,374 bp in length, which contains a large single-copy (LSC) region of 88,446 bp, a small sing-copy (SSC) region of 18,134 bp, and two inverted repeat (IRa and IRb) regions of 26,897 bp. A total of 130 genes were successfully annotated containing 85 protein-coding genes, 37 tRNA genes and 8 rRNA genes. Phylogenetic analysis support genus Ficus is monophyletic and is closely related to within this genus.
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http://dx.doi.org/10.1080/23802359.2019.1689867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707796PMC
November 2019

Descriptive assessment on diabetic retinopathy screening in an awareness programme in Malaysia.

Int J Ophthalmol 2020 18;13(11):1808-1813. Epub 2020 Nov 18.

Institute for Public Health, National Institutes of Health, Ministry of Health, Selangor 40170, Malaysia.

Aim: To determine the prevalence of diabetic retinopathy (DR) among diabetic patients at the primary health clinics in Selangor, Malaysia.

Methods: All diabetic patients were screened in Retinal Disease Awareness Programme (RDAP) and those who had significant DR changes were referred to the hospital for further management. Descriptive analyses were done to determine the prevalence of DR and sociodemographic characteristics among patients with diabetic. Univariate and multivariable analysis using Logistic regression were performed to find association and predictor factors in this screening.

Results: A total of 3305 patients aged 40y and above were screened for DR. Of the patients screened, 9% patients were found to have DR and other visual complication such as maculopathy (0.9%), cataract (4.8%) and glaucoma (0.4%). The mean age of patients without retinopathy was 57.82±8.470y and the mean age of patients with DR was 63.93±9.857y. About 61.5% of the patients screened were aged below 60y and 38.5% were aged 60y and above. Majority of the patients screened were women 58.5% and Malay in the age group of 50-59y, while 27% were aged 60-69y. Significant association were found between age, sex, race, visual loss and DR.

Conclusion: Although the prevalence of DR among patients is not alarming, effective interventions need to be implemented soon to avert a large burden of visual loss from DR.
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http://dx.doi.org/10.18240/ijo.2020.11.19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590874PMC
November 2020
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