Publications by authors named "Ce Wang"

258 Publications

Interface Engineering of Heterogeneous CeO-CoO Nanofibers with Rich Oxygen Vacancies for Enhanced Electrocatalytic Oxygen Evolution Performance.

ACS Appl Mater Interfaces 2021 Sep 22. Epub 2021 Sep 22.

Engineering Research Center of Industrial Biocatalysis, Fujian Province University, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China.

The development of highly efficient and cheap electrocatalysts for the oxygen evolution reaction (OER) is highly desirable in typical water-splitting electrolyzers to achieve renewable energy production, yet it still remains a huge challenge. Herein, we have presented a simple procedure to construct a new nanofibrous hybrid structure with the interface connecting the surface of CeO and CoO as a high-performance electrocatalyst toward the OER through an electrospinning-calcination-reduction process. The resultant CeO-CoO nanofibers exhibit excellent electrocatalytic properties with a small overpotential of 296 mV at 10 mA cm for the OER, which is superior to many previously reported nonprecious metal-based and commercial RuO catalysts. Furthermore, the prepared CeO-CoO nanofibers display remarkable long-term stability, which can be maintained for 130 h with nearly no attenuation of OER activity in an alkaline electrolyte. A combined experimental and theoretical investigation reveals that the excellent OER properties of CeO-CoO nanofibers are due to the unique interfacial architecture between CeO and CoO, where abundant oxygen vacancies can be generated due to the incomplete matching of atomic positions of two parts, leading to the formation of many low-coordinated Co sites with high OER catalytic activity. This research provides a practical and promising opportunity for the application of heterostructured nonprecious metal oxide catalysts for high-efficiency electrochemical water oxidation.
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http://dx.doi.org/10.1021/acsami.1c11101DOI Listing
September 2021

The combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb elicited antitumor immunity by T-cell adoptive immunotherapy in lung cancer.

Int J Med Sci 2021 31;18(15):3380-3388. Epub 2021 Jul 31.

Shenzhen key laboratory of stem cell research and clinical transformation, Guangdong Engineering Technology Research Center of Stem cell and Cell therapy, Translational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, China.

Lung cancer remains a global challenge due to high morbidity and mortality rates and poor response to treatment, and there are still no effective strategies to solve it. The bispecific antibody (BsAb) is a novel antibody, which can target two different antigens and mediate specific killing effects by selectively redirecting effector cells to the target cells. In this study, we combined two BsAbs to achieve a dual-target therapy strategy of EpCAM and MUC-1 with high affinity and specificity. The results showed that the combination of two BsAbs against EpCAM and MUC-1 could inhibit the growth of lung cancer more effectively in cell lines and primary tumors. The superior antitumor effect of two BsAbs could be attributable to enhanced CTL and increased production of type I IFNs. At the same time, the combination of EpCAM/CD3 BsAb and MUC-1/CD3 BsAb significantly regulated T population in the TDLNs. Therefore, we have found a potential immunotherapeutic strategy, which was the combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb for the treatment of non-small cell lung cancer.
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http://dx.doi.org/10.7150/ijms.61681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436090PMC
July 2021

Intraoperative halo-femoral traction during posterior spinal arthrodesis for adolescent idiopathic scoliosis curves between 70° and 100°: a randomized controlled trial.

J Neurosurg Spine 2021 Sep 3:1-8. Epub 2021 Sep 3.

1Department of Orthopedics, and.

Objective: Surgical management of scoliosis curves between 70° and 100° remains controversial. The authors designed this randomized controlled trial to validate the efficacy of intraoperative halo-femoral traction (IOHFT) in patients with adolescent idiopathic scoliosis (AIS), Cobb angles between 70° and 100°, and flexibility < 35%.

Methods: The authors prospectively recruited and randomized 29 patients with severe AIS scheduled for posterior surgery into a traction group or control (nontraction) group. The primary outcome measures were operative time, blood loss, and length of hospital stay. Secondary outcomes included degree of spine deformity correction, traction-related complications, and health-related quality of life.

Results: In the traction group, the average preoperative Cobb angle was 83.2°, with an average 20.6% flexibility. The average postoperative Cobb angle was 16.1° and the major curve was 18.3° at the final follow-up. In the control group, the average preoperative major curve was 80.3° with 22.8% flexibility. The average postoperative Cobb angle was 16.1° and the major curve was 18.1° at the final follow-up. The operative duration was 325.7 minutes for the traction group and 385.4 minutes for the control group (p = 0.018). Compared with the control group, the traction group had a 29.5% reduction in intraoperative blood loss and a significantly lower rate of blood transfusion (13.3% vs 50.0%, p = 0.033). There were no neurological complications in either group. One patient in the traction group had a superficial infection at the traction site.

Conclusions: Use of IOHFT contributed to significant reductions in operative time and blood transfusion requirements, with no added morbidity. It is an effective and safe method to assist correction of AIS curves between 70° and 100° and flexibility < 35%.
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http://dx.doi.org/10.3171/2021.2.SPINE21184DOI Listing
September 2021

Electrospun One-Dimensional Electrocatalysts for Oxygen Reduction Reaction: Insights into Structure-Activity Relationship.

ACS Appl Mater Interfaces 2021 Aug 9;13(32):37961-37978. Epub 2021 Aug 9.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, People's Republic of China.

Oxygen reduction reaction (ORR) is an efficiency-determining process at the cathode in several energy storage and conversion devices, typically such as metal-air batteries and fuel cells. To date, a considerable amount of ORR electrocatalysts have been purposely exploited to address the key issues of high overpotentials and sluggish electrochemical kinetics. Electrospinning is a popular additive manufacturing technology, enabling the production of one-dimensional (1D) electrocatalysts with outstanding chemical stability and structural diversity. However, compared with the well-studied composite/structural design as well as performance advancement, insights into structure-activity relationship are yet to be settled. To clarify this key issue, herein, a dedicated review on the structure-activity relationship between the 1D architectures of electrospun electrocatalysts and their catalytic ORR property is presented. First, the development and principles of electrospinning technique, the composition regulation- and structure design-oriented fundamentals are summarized by imputing the perspectives of mechanistic understanding. Then, the typical examples of nanofiber-shaped and nanofiber-supported electrocatalysts with different compositions and structures for ORR are implemented to establish different structure-activity relationship by comparative studies. Finally, we also identify some ongoing challenges and present future perspectives to direct the precise manipulation of structure-activity relationship for further activation and optimization of electrospun 1D electrocatalysts.
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http://dx.doi.org/10.1021/acsami.1c08798DOI Listing
August 2021

Explainable deep learning predictions for illness risk of mental disorders in Nanjing, China.

Environ Res 2021 Jul 28;202:111740. Epub 2021 Jul 28.

School of Architecture and Urban Planning, Nanjing University, No. 22 Hankoulu Road, Nanjing, 210093, PR China. Electronic address:

Epidemiological studies have revealed the associations of air pollutants and meteorological factors with a range of mental health conditions. However, little is known about local explanations and global understanding on the importance and effect of input features in the complex system of environmental stressors - mental disorders (MDs), especially for exposure to air pollution mixture. In this study, we combined deep learning neural networks (DLNNs) with SHapley Additive exPlanation (SHAP) to predict the illness risk of MDs on the population level, and then provided explanations for risk factors. The modeling system, which was trained on day-by-day hospital outpatient visits of two major hospitals in Nanjing, China from 2013/07/01 through 2019/02/28, visualized the time-varying prediction, contributing factors, and interaction effects of informative features. Our results suggested that NO, SO, and CO made outstanding contributions in magnitude of feature attributions under circumstances of mixed air pollutants. In particular, NO at high concentration level was associated with an increase in illness risk of MDs, and the maximum and mean absolute SHAP value were approximated to 10 and 2 as a local and global measure of feature importance, respectively. It presented a marginally antagonistic effect for two pairs of gaseous pollutants, i.e., NO vs. SO and CO vs. NO. In contrast, CO and SO displayed the opposite direction of feature effects to the rise of observed concentrations, but an apparent synergistic effect was obviously captured. The primary risk factors driving a sharp increase in acute attack or exacerbation of MDs were also identified by depicting prediction paths of time-series samples. We believe that the significance of coupling accurate predictions from DLNNs with interpretable explanations of why a prediction is completed has broad applicability throughout the field of environmental health.
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http://dx.doi.org/10.1016/j.envres.2021.111740DOI Listing
July 2021

Construction of a pH-sensitive self-assembly in aqueous solutions based on a dansyl-modified β-cyclodextrin.

Soft Matter 2021 Aug 28;17(32):7516-7523. Epub 2021 Jul 28.

School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.

Here we present a pH-responsive self-assembly based on a β-cyclodextrin (β-CD) derivative bearing a dansyl terminus (βCD-C-Dns). Vesicular structures were formed over the entire studied pH range (8.5-0.7); however, the molecular configuration and packing within the vesicles were different at different pH values. Intramolecular host-guest complexation occurred mainly between the dansyl group and β-CD at pH values where the dansyl group was not protonated. The alkyl chain also acted as a competitive guest to form host-guest inclusions as confirmed by 2D H NMR measurements. The pH-responsive βCD-C-Dns vesicles have potential application prospects in pH-controlled drug release based on the low cytotoxicity of βCD-C-Dns.
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http://dx.doi.org/10.1039/d1sm00751cDOI Listing
August 2021

Disentangled Feature Learning Network and a Comprehensive Benchmark for Vehicle Re-Identification.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 26;PP. Epub 2021 Jul 26.

Large and comprehensive datasets are crucial for the development of vehicle ReID. In this paper, we propose a large vehicle ReID dataset, called VERI-Wild 2.0, containing 825,042 images. It is captured using a city-scale surveillance camera system, which consists of 274 cameras covering 200 km. Specifically, the samples in our dataset present rich diversities thanks to the long time span collecting settings, unconstrained capturing viewpoints, various illumination conditions, and diversified background environments. Furthermore, we define a challenging test set containing about 400K vehicle images that do not have any camera overlap with the training set. Besides, we also design a new method. We observe that the orientation is a crucial factor for vehicle ReID. To match vehicle pairs captured from similar orientations, the learned features are expected to capture specific detailed differential information, while features are desired to capture the orientation invariant common information when matching samples captured from different orientations. Thus a novel disentangled feature learning network(DFNet) is proposed. It explicitly considers the orientation information for vehicle ReID, and concurrently learns the orientation specific and common features that thus can be adaptively exploited via a hybrid ranking strategy when dealing with different matching pairs. The comprehensive experimental results show the effectiveness of our proposed method.
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http://dx.doi.org/10.1109/TPAMI.2021.3099253DOI Listing
July 2021

Single-cell Long Non-coding RNA Landscape of T Cells in Human Cancer Immunity.

Genomics Proteomics Bioinformatics 2021 Jul 17. Epub 2021 Jul 17.

Translational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, China; Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation, Shenzhen 518020, China. Electronic address:

The development of new biomarkers or therapeutic targets for cancer immunotherapies requires deep understanding of T cells. To date, the complete landscape and systematic characterization of long noncoding RNAs (lncRNAs) in T cells in cancer immunity are lacking. Here, by systematically analyzing full-length single-cell RNA sequencing (scRNA-seq) data of more than 20,000 libraries of T cells across three cancer types, we provide the first comprehensive catalog and the functional repertoires of lncRNAs in human T cells. Specifically, we developed a custom pipeline for de novo transcriptome assembly and obtained a novel lncRNA catalog containing 9433 genes. This increased the number of current human lncRNA catalog by 16% and nearly doubled the number of lncRNAs expressed in T cells. We found that a portion of expressed genes in single T cells were lncRNAs which had been overlooked by the majority of previous studies. Based on metacell maps constructed by the MetaCell algorithm that partitions scRNA-seq datasets into disjointed and homogenous groups of cells (metacells), 154 signature lncRNAs were identified. They associated with effector, exhausted, and regulatory T cell states. 84 of them were functionally annotated based on the co-expression network, indicating that lncRNAs might broadly participate in the regulation of T cell functions. Our findings provide a new point of view and resource for investigating the mechanisms of T cell regulation in cancer immunity as well as for novel cancer-immune biomarker development and cancer immunotherapies.
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http://dx.doi.org/10.1016/j.gpb.2021.02.006DOI Listing
July 2021

The Complementary Relationship Between Echocardiography and Multi-Slice Spiral CT Coronary Angiography in the Diagnosis of Coronary Artery Thrombosis in Children With Kawasaki Disease.

Front Pediatr 2021 30;9:670887. Epub 2021 Jun 30.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.

To compare the diagnostic values by using transthoracic echocardiography (ECHO) and multi-slice spiral CT coronary angiography (CTCA) for identifying coronary artery thrombosis in children with Kawasaki disease (KD). Total 97 KD children with coronary artery dilation complications in our hospital from June 2012 to December 2020 were included in the study. CTCA and ECHO were performed after over 1 month of illness. Coronary artery thrombosis was found in 14 out of 97 patients. Among them, 10 were identified as positive by CTCA, 9 were identified as positive by ECHO, and 5 were identified as positive by both CTCA and ECHO. Both CTCA and ECHO can be used to diagnose coronary artery thrombosis. ECHO has advantage in identifying low-density thrombus, and CTCA is better for the clot in distal coronary artery. They can complement each other.
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http://dx.doi.org/10.3389/fped.2021.670887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278001PMC
June 2021

Effect of Bile Salts on the Interfacial Dilational Rheology of Lecithin in the Lipid Digestion Process.

J Oleo Sci 2021 Aug 9;70(8):1069-1080. Epub 2021 Jul 9.

School of Light Industry, Beijing Technology and Business University.

The effects of bile salts on the emulsifier adsorption layer play a crucial role in lipid digestion. The current study selected sodium cholate (NaCh) and lecithin as model compounds for bile salts and food emulsifiers, respectively. The interface dilational rheological and emulsification properties of NaCh and lecithin were carried out. The results showed that the NaCh molecules could quickly diffuse from the bulk to interface, which broke the tightly-arranged interfacial layer of lecithin and enhanced the viscoelasticity of interfacial film. As a result, the interfacial adsorption layer, which was originally dominated by the slow relaxation processes within the interface, was transformed into one controlled by the fast molecular diffusion exchange. This accelerated the exchange of materials between the bulk and interface, thereby creating suitable conditions for the interfacial adsorption of lipases, which promoted the digestion process. These results provided a mechanism for the promotion of lipid digestion by bile salts from the perspective of interfacial viscoelasticity and relaxation processes. A deeper understanding of the interfacial behavior of bile salts with emulsifiers would provide a basis for the rational design of interfacial layer for modulating lipid digestion.
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http://dx.doi.org/10.5650/jos.ess21081DOI Listing
August 2021

Hierarchical Connectivity-Centered Clustering for Unsupervised Domain Adaptation on Person Re-Identification.

IEEE Trans Image Process 2021 26;30:6715-6729. Epub 2021 Jul 26.

Unsupervised domain adaptation (UDA) on person Re-Identification (ReID) aims to transfer the knowledge from a labeled source domain to an unlabeled target domain. Recent works mainly optimize the ReID models with pseudo labels generated by unsupervised clustering on the target domain. However, the pseudo labels generated by the unsupervised clustering methods are often unreliable, due to the severe intra-person variations and complicated cluster structures in the practical application scenarios. In this work, to handle the complicated cluster structures, we propose a novel learnable Hierarchical Connectivity-Centered (HCC) clustering scheme by Graph Convolutional Networks (GCNs) to generate more reliable pseudo labels. Our HCC scheme learns the complicated cluster structure by hierarchically estimating the connectivity among samples from the vertex level to cluster level in a graph representation, and thereby progressively refines the pseudo labels. Additionally, to handle the intra-person variations in clustering, we propose a novel relation feature for HCC clustering, which exploits the identities from the source domain as references to represent target domain samples. Experiments demonstrate that our method is able to achieve state-of-the art performance on three challenging benchmarks.
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http://dx.doi.org/10.1109/TIP.2021.3094140DOI Listing
July 2021

General fabrication of metal-organic frameworks on electrospun modified carbon nanofibers for high-performance asymmetric supercapacitors.

J Colloid Interface Sci 2021 Dec 26;603:199-209. Epub 2021 May 26.

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, NO. 2699 Qianjin Street, Changchun 130012, PR China. Electronic address:

Metal-organic framework (MOF)-based electrode materials have become a hot subject for supercapaitors. Herein, Ni-MOFs grown on Co nanoparticles modified carbon nanofibers (CNFs) ([email protected]) are prepared via a facile process. Interestingly, the presence of Co nanoparticles in CNFs not only boosts the hybridization of CNF and MOFs, but also releases Co ions to participate in the growth of MOF, leading to a favorable electrochemical behavior. In detail, the specific capacitance of [email protected] reaches 1201.6 F g that exceeds those of [email protected] (M = Ni, V, Mo, Mn, Fe, Cu and Zn) and [email protected] More importantly, an asymmetric solid-state supercapacitor is assembled using [email protected] and nitrogen-doped carbon nanotubes derived from polyaniline as positive and negative electrode materials, respectively, representing a high energy density of 37.0 Wh kg and outstanding durability. This work highlights the superiority of electrospun CNFs modified by metal nanoparticles for the growth of MOF, showing great potential for electrochemical energy storage and conversion applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.138DOI Listing
December 2021

pH-Dependent complexation between β-lactoglobulin and lycopene: Multi-spectroscopy, molecular docking and dynamic simulation study.

Food Chem 2021 Nov 28;362:130230. Epub 2021 May 28.

Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun 130062, China. Electronic address:

This study aims to investigate the effect of pH levels (pH 7.0 and pH 8.1) on binding ability of β-lactoglobulin (β-LG) with lycopene (LYC) and elucidate interaction mechanisms using multi-spectroscopy and molecular docking study. β-LG at pH 8.1 showed a stronger binding affinity to lycopene than that at pH 7.0 according to binding constant, binding number, energy transfer efficiency, and surface hydrophobicity. Lycopene bound to protein mainly by van der Waals force in the form of static quenching mode and preferred to interact with β-LG at the top of barrel for both pH levels. Molecular dynamic simulation revealed that β-LG/LYC complex at pH 8.1 was more stable than at pH 7.0. β-LG/LYC complexes formed at pH 8.1 showed significantly higher ABTS radical scavenging activity than samples at pH 7.0 (p < 0.05). Data obtained may contribute valuable information for preparing a whey protein-based delivery system for lycopene.
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http://dx.doi.org/10.1016/j.foodchem.2021.130230DOI Listing
November 2021

Liposomal honokiol promotes hair growth via activating Wnt3a/β-catenin signaling pathway and down regulating TGF-β1 in C57BL/6N mice.

Biomed Pharmacother 2021 Sep 5;141:111793. Epub 2021 Jun 5.

Department of Neuro-oncology, Cancer Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, China. Electronic address:

Liposomal honokiol isolated from the genus Magnolia has been found to have antiangiogenic, anti-inflammatory and antitumor properties. However, there has no report on its role in hair growth. Hair follicles are life-long cycled organelles that go through from anagen, catagen and telogen stages and are regulated by diverse signaling pathways, including Wnt/β-catenin, Notch, Epidermal growth factor (EGF) and Sonic hegehog (SHH). Wnt signals are essential for the initiation of hair follicle placode development and a new potential target of hair loss treatment. This study was designed to investigate the effect of liposomal honokiol (Lip-honokiol) on inducing hair anagen. We identified the hair grew out in advance in the shaving area of C57BL/6N mice after the treatment of liposomal honokiol (Lip-honokiol) by daily abdominal injection. We first demonstrated that Lip-Honokiol activated the Wnt3a/β-catenin pathway and downregulated the transforming growth factor-β1 (TGF-β1) to promote hair growth in mice via immunohistochemistry and immunofluorescence staining. These findings suggest that Lip-honokiol activated the Wnt/β-catenin pathway and accelerated the transfer from the telogen to anagen stage and finally promoted the hair growth.
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http://dx.doi.org/10.1016/j.biopha.2021.111793DOI Listing
September 2021

Palladium cobalt alloy encapsulated in carbon nanofibers as bifunctional electrocatalyst for high-efficiency overall hydrazine splitting.

J Colloid Interface Sci 2021 Nov 25;601:495-504. Epub 2021 May 25.

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address:

Electrolytic water splitting is a promising strategy to generate clean hydrogen energy but still restricted by the sluggish kinetics during the anodic oxygen evolution reaction (OER). A highly efficient route to significantlyreduce the cell voltage of electrolytic water splitting is to replace OER with hydrazine oxidation reaction (HzOR) so as to assist hydrogen generation effectively. Here, we report the fabrication of carbon nanofibers (CNFs) embedded with palladium cobalt (PdCo) alloy nanoparticles, via an electrospinning followed by a carbonization treatment. The as-synthesized PdCo-CNFs catalyst displays a superior electrocatalytic activity toward HzOR with a working potential of 258 mV (vs. RHE) to drive a current density of 50 mA cm in an alkaline solution with 0.2 M hydrazine. Furthermore, the favorable hydrogen evolution reaction (HER) activity of this catalyst enables it highly efficient electrolytic hydrogen production, and the two-electrode system using PdCo-CNFs as both the cathode and anode for overall hydrazine splitting is capable of delivering a cell voltage of 0.440 V to attain 10 mA cm, which is 1.496 V less than that for pure water splitting using the same electrodes and even 0.459 V less than the overall hydrazine splitting device using Pt/C//RuO as electrocatalysts. This work provides a reliable way for the fabrication of promising bifunctional electrocatalysts to promote energy-saving hydrogen production for practical applications.
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http://dx.doi.org/10.1016/j.jcis.2021.05.119DOI Listing
November 2021

A dansyl-based fluorescent probe for sensing Cu in aqueous solution.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 26;261:120009. Epub 2021 May 26.

School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.

A fluorescent probe based on a glycyl-L-tyrosine-modified dansyl derivative (D-GT) is designed and synthesized. D-GT demonstrated great detection performance toward Cu in an aqueous solution. Fluorescence quenching occurred due to the coordination of Cu with D-GT. The sensitive detection of D-GT to Cu was applied in aqueous solution within a wide pH span (6-12). A 1:1 coordinate stoichiometric way and an association constant of 6.47 × 10 M between D-GT and Cu were determined. The measured detection limit for Cu in HEPES buffer solution (10 mM, pH 7.4) was 0.69 μM. The probe displayed an appropriate sensitivity toward Cu in real drinking water samples and living cells, which reveals the potential applications of D-GT in complicated environments.
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http://dx.doi.org/10.1016/j.saa.2021.120009DOI Listing
November 2021

Transition metal sulfides meet electrospinning: versatile synthesis, distinct properties and prospective applications.

Nanoscale 2021 May;13(20):9112-9146

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.

One-dimensional (1D) electrospun nanomaterials have attracted significant attention due to their unique structures and outstanding chemical and physical properties such as large specific surface area, distinct electronic and mass transport, and mechanical flexibility. Over the past years, the integration of metal sulfides with electrospun nanomaterials has emerged as an exciting research topic owing to the synergistic effects between the two components, leading to novel and interesting properties in energy, optics and catalysis research fields for example. In this review, we focus on the recent development of the preparation of electrospun nanomaterials integrated with functional metal sulfides with distinct nanostructures. These functional materials have been prepared via two efficient strategies, namely direct electrospinning and post-synthesis modification of electrospun nanomaterials. In this review, we systematically present the chemical and physical properties of the electrospun nanomaterials integrated with metal sulfides and their application in electronic and optoelectronic devices, sensing, catalysis, energy conversion and storage, thermal shielding, adsorption and separation, and biomedical technology. Additionally, challenges and further research opportunities in the preparation and application of these novel functional materials are also discussed.
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http://dx.doi.org/10.1039/d1nr01070kDOI Listing
May 2021

Molecular characterization of four novel serpins in Psoroptes ovis var. cuniculi and their implications in the host-parasite interaction.

Int J Biol Macromol 2021 Jul 14;182:1399-1408. Epub 2021 May 14.

Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, People's Republic of China.

Psoroptes ovis var. cuniculi infestation rapidly causes skin lesion, cutaneous inflammatory and subsequent adaptive immune response in rabbits. To success feeding and survive on the host skin, this mite should product bioactive molecules to confront host tissue repair and immune defense, but these molecules of this mite remains mostly unknown. Serpins have been proved to involve in diverse biological functions including parasite reproduction, survival and modulating host defense. Limited information is currently available on serpins from Psoroptes mites. Herein, we identified four novel serpins (PsoSP3-PsoSP6) in P. ovis var. cuniculi using bioinformatics and molecular biology techniques. Sequence analysis revealed that PsoSP3-PsoSP6 comprised the common features of typical serpins superfamily including serpin domains, signature or the reactive centre loop (RCL) domain. The recombinant PsoSP4-PsoSP6 (rPsoSP4-rPsoSP6) revealed variable potency inhibition on trypsin, chymotrypsin and elastase except for rPsoSP3 in inhibitory activity assays. By quantitative RT-PCR, the expressions of PsoSP3 and PsoSP4 were higher in juvenile mites (larva and nymph) than in adult mites, however, PsoSP5 and PsoSP6 appeared near-exclusive expression in adult female mites. Immunolocalization showed that native PsoSP4 protein was localized in uterus, whilst native PsoSP3, PsoSP5 and PsoSP6 were specifically localized in the ovarian nutritive cell (ONC) in ovary. Our findings indicated that PsoSP3-PsoSP6 might play critical roles in development and reproduction physiologies. rPsoSP4-rPsoSP6 might participate in modulating host inflammation, immune response and tissue repair.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.090DOI Listing
July 2021

A validated nomogram integrating hematological indicators to predict response to neoadjuvant therapy in esophageal squamous cell carcinoma patients.

Ann Transl Med 2021 Apr;9(8):703

Department of Thoracic Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: The prognoses for advanced Esophageal squamous cell cancer (ESCC) was very poor. Neoadjuvant therapy was shown to improve overall survival of ESCC patients. However, there is still no effective indicator to predict the efficacy of neoadjuvant therapy. The present study intended to investigate the correlation between hematological parameters and the efficacy of neoadjuvant therapy so as to provide a reference for the prediction of cancer response to neoadjuvant therapy.

Methods: This study included 197 ESCC patients in our center from January 2010 to December 2018. Response evaluation criteria in solid tumors (RECIST) criteria were used for the treatment evaluation. The results of univariate and multivariate logistic regression analysis were used to select independent factors for construction of the prediction model. The concordance index (C-index), receiver operating characteristic (ROC) curve, and calibration curve were used to evaluate the robustness of the model, while the bootstrap method was used for internal validation.

Results: Among the 197 included ESCC patients, 94 patients achieved partial remission, 80 patients were in stable condition, and 23 patients had disease progression, 123 of whom underwent surgery. The comparisons of the dynamic hematological test results before and after treatment show that pre-PLT, pre-MONO%, post-Hb, △WBC, and the option of undergoing neoadjuvant chemoradiation were the potential predictors for the effectiveness of neoadjuvant therapy. The model in which the C-index was 0.803 (95% confidence interval: 0.742-0.864) showed good prediction performance, and still reach a C-index of 0.764 when internally validated.

Conclusions: For the neoadjuvant treatment of ESCC, hematological indexes are closely related to the efficacy of neoadjuvant therapy. The nomogram can be used to easily predict the efficacy of neoadjuvant therapy in patients.
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http://dx.doi.org/10.21037/atm-21-1628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106100PMC
April 2021

Demonstration of optical gain at 1550 nm in an Er-Yb co-doped phosphate planar waveguide under commercial and convenient LED pumping.

Opt Express 2021 Apr;29(8):11372-11385

A 980 nm semiconductor laser is always selected as the pump source for erbium-ytterbium co-doped optical waveguide amplifiers. In this work, two low-cost blue-violet LEDs, rather than an expensive 980 nm laser, were used to pump an Er-Yb co-doped phosphate planar waveguide. When the signal power was 0.4 mW at a 1550 nm wavelength, internal optical gains of about 4.1 and 4.5 dB/cm were respectively obtained under the excitations of a 32 mW/cm, 275 nm LED and a 914 mW/cm, 405 nm LED. It was found that 51.17% of the total Er ions in the H state contributed to the luminescence at 1550 nm, and a theoretical model of gain simulation was established under the excitation of a 405 nm LED. The calculated gain of about 4.1 dB/cm was found to be in accordance with the experimental optical gain results.
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http://dx.doi.org/10.1364/OE.414847DOI Listing
April 2021

Sheathless microflow cytometer utilizing two bulk standing acoustic waves.

Cytometry A 2021 May 6. Epub 2021 May 6.

School of Biomedical Engineering(Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Suzhou, Jiangsu, China.

In recent years, microflow cytometry has become a popular research field because of its potential to provide low-cost and disposable chips for complex cell analyses. Herein, we demonstrate a sheathless microflow cytometer which integrates a bulk standing acoustic wave based microchip capable of three dimensional cell focusing. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of 2.16% with standard calibration beads. The sensitivities calibrated by rainbow beads are 518 MEFL in fluorescein Isothiocyanate (FITC) channel and 264 MEPE in P-phycoerythrin (PE) channels, respectively. The linearities are more than 99% in both channels. The capability of the proposed microflow cytometer is further demonstrated by immunologically labeled leukocytes differentiation in blood. This acoustic-based microflow cytometer did not require any sheath flows or complex structures and can be mass produced. Because of the simple fluid channel, the chip can be easily made pipeless, disposable for applications requiring no cross contamination. Moreover, with the gentle and bio-compatible acoustic waves used, this technique is expected to maintain the viability of cells and other bioparticles.
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http://dx.doi.org/10.1002/cyto.a.24362DOI Listing
May 2021

Photoperiod-responsive changes in chromatin accessibility in phloem companion and epidermis cells of Arabidopsis leaves.

Plant Cell 2021 05;33(3):475-491

Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, and College of Life Sciences, Capital Normal University, Beijing 100048, China.

Photoperiod plays a key role in controlling the phase transition from vegetative to reproductive growth in flowering plants. Leaves are the major organs perceiving day-length signals, but how specific leaf cell types respond to photoperiod remains unknown. We integrated photoperiod-responsive chromatin accessibility and transcriptome data in leaf epidermis and vascular companion cells of Arabidopsis thaliana by combining isolation of nuclei tagged in specific cell/tissue types with assay for transposase-accessible chromatin using sequencing and RNA-sequencing. Despite a large overlap, vasculature and epidermis cells responded differently. Long-day predominantly induced accessible chromatin regions (ACRs); in the vasculature, more ACRs were induced and these were located at more distal gene regions, compared with the epidermis. Vascular ACRs induced by long days were highly enriched in binding sites for flowering-related transcription factors. Among the highly ranked genes (based on chromatin and expression signatures in the vasculature), we identified TREHALOSE-PHOSPHATASE/SYNTHASE 9 (TPS9) as a flowering activator, as shown by the late flowering phenotypes of T-DNA insertion mutants and transgenic lines with phloem-specific knockdown of TPS9. Our cell-type-specific analysis sheds light on how the long-day photoperiod stimulus impacts chromatin accessibility in a tissue-specific manner to regulate plant development.
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http://dx.doi.org/10.1093/plcell/koaa043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136901PMC
May 2021

Reliability and Validity of the Adapted Chinese Version of the Satisfaction of Adolescents with Postoperative Pain Management - Idiopathic Scoliosis (SAP-S) Scale.

J Pain Res 2021 12;14:953-960. Epub 2021 Apr 12.

Department of Orthopedics, Changzheng Hospital, Naval Medical University, Shanghai, 200003, People's Republic of China.

Study Design: A prospective study.

Objective: The aim of this study was to evaluate the internal reliability and structure validity of an adapted simplified Chinese version of the Satisfaction of Adolescents with Postoperative pain management - idiopathic Scoliosis (SAP-S) scale in mainland China.

Summary Of Background Data: Pain management is a major issue for adolescent idiopathic scoliosis (AIS) patients undergoing posterior spinal fusions. There is a lack of valid scales for evaluating patients' satisfaction with postoperative pain management. The SAP-S was proven to be a valid and reliable measure in English and French.

Methods: The SAP-S was translated into Chinese according to the internationally recognized guidelines. A total of 95 AIS patients undergoing posterior fusion surgery completed the CSAP-S, along with other self-reported questionnaires, including the 36-Item Short Form Health Survey (SF-36) and Scoliosis Research Society-22 (SRS-22) questionnaires. The internal consistency, test-retest reliability, and construct validity of the CSAP-S were determined.

Results: The SAP-S was successfully translated into Chinese. All patients completed the CSAP-S twice and the other instruments. The CSAP-S had good internal consistency and test-retest reliability with Cronbach's alpha coefficient measuring 0.895 and intraclass correlation coefficient (ICC) measuring 0.97. Elimination of any one item did not result in a value of Cronbach's alpha of <0.80. A good construct validity was shown by good correlation with bodily pain (r=0.883, p=0.004) and social functioning (r=0.786, p=0.002) domains of SF-36 and pain (r=0.752, p=0.001) and satisfaction with management (r=0.746, p=0.005) domains of SRS-22.

Conclusion: The CSAP-S demonstrated good internal consistency, reliability, and construct validity, and may be used for the evaluation of AIS patients' satisfaction with postoperative pain management in mainland China.
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http://dx.doi.org/10.2147/JPR.S301205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052124PMC
April 2021

Targeted exome sequencing identifies mutational landscape in a cohort of 1500 Chinese patients with non-small cell lung carcinoma (NSCLC).

Hum Genomics 2021 Apr 12;15(1):21. Epub 2021 Apr 12.

Department of Radiation Oncology, The First Affiliated Hospital of Anhui Medical University, No.218, Jixi Road, Hefei, 230022, Anhui, China.

Background: Non-small cell lung carcinoma (NSCLC) is one of the most common human cancers, comprising approximately 80-85% of all lung carcinomas. An estimated incidence of NSCLC is approximately 2 million new cases per year worldwide.

Results: In recent decade, the treatment of NSCLC has made breakthrough progress owing to a large number of targeted therapies which were approved for clinical use. Epidemiology, genetic susceptibility, and molecular profiles in patients are likely to play an important factor in response rates and survival benefits to these targeted treatments and thus warrant further investigation on ethnic differences in NSCLC. In this study, a total number of 1500 Chinese patient samples,1000 formalin fixed paraffin-embedded (FFPE) and 500 blood samples, from patients with NSCLC were analyzed by targeted sequencing to explore mutational landscape in ethnic groups associated with China.

Conclusions: Overall, the data presented here provide a comprehensive analysis of NSCLC mutational landscape in Chinese patients and findings are discussed in the context of similar studies on different ethnic groups.
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http://dx.doi.org/10.1186/s40246-021-00320-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042687PMC
April 2021

Optimal design of a driver of interdigital transducers used to generate standing surface acoustic waves for cell sorting.

Rev Sci Instrum 2021 Mar;92(3):034705

University of Sciences and Technology of China, Hefei, Anhui 230026, China.

A compact driver based on current feedback amplifiers is designed to drive interdigital transducers (IDTs) that generate standing surface acoustic waves for cell sorting. Compared with commercial RF amplifiers, this driver can be used to drive a wider range of loads without impedance matching. Furthermore, the driver works in a switch mode triggered by target cells, which significantly reduces power consumption in the system. A Butterworth-Van Dyke equivalent circuit was fabricated to study the electrical characteristics of the IDTs, and the driver was designed and optimized by circuit simulations. A cell sorter was constructed and tested experimentally to demonstrate that the driver meets sorting requirements. The driver allows the cell sorter to extract rare cells while otherwise consuming low power.
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http://dx.doi.org/10.1063/5.0036856DOI Listing
March 2021

Genomic insights into evolution of pathogenicity and resistance of multidrug-resistant Raoultella ornithinolytica WM1.

Ann N Y Acad Sci 2021 Aug 30;1497(1):74-90. Epub 2021 Mar 30.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.

Raoultella ornithinolytica is a poorly understood opportunistic pathogen, and the underlying mechanisms of its multidrug resistance and pathogenicity have not yet been comprehensively investigated. The multidrug-resistant (MDR) strain WM1 was isolated from the blood of a male patient in Tianjin, China, in 2018. Here, we describe the complete genome and provide a genomic analysis of R. ornithinolytica WM1. The isolate was resistant to all tested antimicrobials except amikacin, tobramycin, and tigecycline. Two plasmids, pWM1-1 (IncHI5) and pWM1-2 (IncR), carried multidrug-resistance regions. A large antimicrobial resistance island region resided on pWM1-1 and exhibited mosaic structures resulting from the acquisition of complex integrations of variable regions, including genes conferring resistance to multiple classes of antimicrobials. Moreover, WM1 possessed virulence-related elements that encode several virulence factors, including type I fimbriae, Escherichia coli common pilus, type II and VI secretion systems, yersiniabactin, enterobactin, and surface polysaccharide, indicating pathogenic potential. Furthermore, the core genome phylogeny and pan-genome analyses revealed extensive genetic diversity. Our analysis indicates the need for stringent infection control, antimicrobial stewardship, periodic resistance monitoring, and rational medication to address potential threats posed by MDR R. ornithinolytica strains.
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http://dx.doi.org/10.1111/nyas.14595DOI Listing
August 2021

FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.

Metabolism 2021 06 20;119:154767. Epub 2021 Mar 20.

Department of Orthopedics, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, People's Republic of China. Electronic address:

Background: Obesity and osteoporosis frequently coexist, and might have a causal relationship. Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss. The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.

Methods: Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks. Fructooligosaccharides (FOS) and/or galactooligosaccharides (GOS) were daily gavaged to mice. Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice. The composition of gut flora was evaluated by 16s rDNA sequencing. Expression of intestinal junction proteins was assessed by qPCR and immunohistochemistry. Cytokine levels were measured by qPCR.

Results: Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis. FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts. In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.

Conclusion: Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation. The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.
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http://dx.doi.org/10.1016/j.metabol.2021.154767DOI Listing
June 2021

Impact of polymerized whey protein/pectin thickening (PP) system on physical properties and volatile compounds of goat milk kefir mild and kefir.

J Food Sci 2021 Mar 2;86(3):1014-1021. Epub 2021 Feb 2.

Department of Nutrition and Food Sciences, College of Agriculture and Life Sciences, University of Vermont, Burlington, Vermont, 05405, USA.

Polymerized whey protein and low-methoxyl pectin were used as thickening agents in fermented dairy food formulations. Effects of polymerized whey protein and pectin (PP) system on particle size distributions, rheological properties, molecular weight distributions of polysaccharides, microstructures, and volatile compounds of goat milk kefir mild and kefir were investigated. Clustering analysis indicated that the PP system addition could increase the particle size distributions and rheological properties of both kefir products. Molecular weight distributions of polysaccharides shown that an intermolecular aggregation occurred between pectin and exopolysaccharides (produced by microorganisms from starter cultures). Micrographs suggested that polymerized whey protein interacted with casein network to form a dense structure. Meanwhile, PP system did not change the characteristics flavor of goat milk kefir mild and kefir. Results indicated that the PP system may be a good combination to improve the physical properties of kefir products. PRACTICAL APPLICATION: Goat milk kefir has gained more and more attention due to its specific flavor and functional properties. However, poor texture of milk curd limits the consumption of fermented goat milk. As a new combination of thickening agents, PP system could specifically improve the particle size distributions and rheological properties of fermented goat milk. And the characteristic flavor of goat milk kefir was not changed. Results indicated that PP system can be used as thickening agents for formulations of different fermented goat milk products.
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http://dx.doi.org/10.1111/1750-3841.15611DOI Listing
March 2021

Two-dimensional poly(3,4-ethylenedioxythiophene) nanosheets for highly electrochemical detection of iodide ions.

Anal Chim Acta 2021 Feb 8;1144:122-129. Epub 2020 Dec 8.

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun, 130012, China. Electronic address:

Two-dimensional (2D) nanomaterials-modified electrodes are good candidates for electrochemical sensing because of their unique ultrathin sheet-like structure and distinctive electrical property. In this work, we have developed a facile sacrificial template-directed mild polymerization process to prepare 2D poly(3,4-ethylenedioxythiophene) (PEDOT) nanosheets. During the polymerization process, VO·nHO nanosheets are used as both sacrificial templates and oxidants, which can not only guide the production of PEDOT nanosheets, but also spontaneously be removed after the reaction. We have demonstrated the usage of the 2D PEDOT nanosheets for electrochemical sensing of iodide ions. The proposed sensor delivers a low detection limit of 0.313 μM (S/N = 3) with a linear range of 1.0-20 μM. Furthermore, the 2D PEDOT-based sensor shows an exciting reproducibility, stability and selectivity for the detection of iodide ions, which can be feasibly applied for the detection in real samples. This study provides a facile route to fabricate 2D conducting polymer-based nanomaterials for efficient electrochemical sensing application.
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http://dx.doi.org/10.1016/j.aca.2020.12.010DOI Listing
February 2021

Constructing Mesoporous Adsorption Channels and MOF-Polymer Interfaces in Electrospun Composite Fibers for Effective Removal of Emerging Organic Contaminants.

ACS Appl Mater Interfaces 2021 Jan 29;13(1):755-764. Epub 2020 Dec 29.

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Recently, metal-organic framework (MOF)-based electrospun fibers have attracted considerable attention as adsorbents for organic contaminant removal from water. To prepare these fibers, two common strategies including blending electrospinning and surface coating are employed. However, fibers obtained from the two strategies still have some disadvantages, such as adsorption site blockage and unstable loading. Here, we constructed interconnected mesopores in the electrospun zeolitic imidazolate framework-8 (ZIF-8)/polyacrylonitrile (PAN) fibers with the assistance of poly(vinylpyrrolidone) to expose more adsorption sites of ZIF-8 and make ZIF-8 more stable. Moreover, the mesopores could also enhance the diffusion of contaminant molecules and create MOF-polymer interfaces in the fiber, which improve the adsorption rate and adsorption capacity, respectively. The obtained fibers were used to adsorb antibiotic tetracycline from water. Benefiting from the mesoporous adsorption channels and the MOF-polymer interface, porous ZIF-8/PAN fibers showed faster adsorption kinetics than ZIF-8/PAN blending fibers and larger adsorption capacity than ZIF-8-coated PAN fibers and ZIF-8/PAN blending fibers. The maximum adsorption capacity of porous ZIF-8/PAN fibers was 885.24 mg/g, which is close to that of pure ZIF-8. After 10 adsorption-desorption cycles, the removal efficiency was still above 97%. In addition, porous ZIF-8/PAN fibers could act as the membrane adsorbents to dynamically separate tetracycline with a treated capacity of 9.93 × 10 bed volumes. These results demonstrate that our prepared porous ZIF-8/PAN fibers have great potential in antibiotic drug removal.
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http://dx.doi.org/10.1021/acsami.0c20404DOI Listing
January 2021
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